ChromatographyPrepared by Eric Ittah

Chromatography is a process that involves the separation of mixtures into their respective components to identify, study or purify the mixture or components.

Definition

Chromatography is derived from the Greek word “Chroma”, meaning colors and “Graphein” which means to write.

Derivation of the word Chromatography

• Pharmaceutical industry• Healthcare services• Research and Analysis• Forensics Science

Applications of Chromatography

• Gas• Paper• Liquid• Affinity• Ion Exchange• Partition• Adsorption

Types of Chromatography

Separation of compounds that can be vaporized without decomposition.It can be used to prepare pure compounds from a mixture.

Gas Chromatography

Paper chromatography is an analytical method used to separate and identify mixtures that can be colored, mainly the pigments.

Paper Chromatography

Liquid chromatography is used to separate ions and molecules that are dissolved in a solvent.

Liquid Chromatography

Affinity chromatography is a method of separating biochemical mixtures based on a highly specific interaction .

Affinity Chromatography

Ion exchange chromatography is a process that allows the separation of ions and polar molecules based on their charge.

Ion Exchange Chromatography

Partition chromatography is a method of separating mixtures according to their different partition coefficients.

Partition Chromatography

Adsorption chromatography is a process of separating mixtures in which solute molecules bond directly to the surface of the stationary phase.

Adsorption Chromatography

Contact Eric Ittah eric@ericittah.ca

Thank You