Cho series

Kool – Fattet – McCabe series

Thompson series

Northcott series

Remke series

WNT SHHGroup 3 Group 4

Supplementary Figure S1 - NMF cluster analyses

All patients GEP All patients TMA All patients GEP + TMA

All infants GEP All infants TMA All infants GEP + TMA

All children GEP All children TMA All children GEP + TMA

All adults GEP All adults TMA All adults GEP + TMA

WNT SHH GRP3 GRP4

Figure S2a: Subgroup distributions GEP and TMA cohorts

WNT SHH GRP3 GRP40

100

200

WNT SHH GRP3 GRP40

50

100

WNT SHH GRP3 GRP40

100

200

WNT SHH GRP3 GRP40

50

100

WNT SHH GRP3 GRP40

100

200

WNT SHH GRP3 GRP40

50

100

WNT SHH GRP3 GRP40

100

200

WNT SHH GRP3 GRP40

50

100

WNT SHH GRP3 GRP40

200

400

WNT SHH GRP3 GRP40

50

100

WNT SHH GRP3 GRP40

200

400

WNT SHH GRP3 GRP40

100

200

All patients GEP All patients TMA All patients GEP + TMA

All infants GEP All infants TMA All infants GEP + TMA

All children GEP All children TMA All children GEP + TMA

All adults GEP All adults TMA All adults GEP + TMA

Figure S2b: Subgroup distributions GEP and TMA cohorts

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

All patients GEP All patients TMA All patients GEP + TMA

All infants GEP All infants TMA All infants GEP + TMA

All children GEP All children TMA All children GEP + TMA

All adults GEP All adults TMA All adults GEP + TMA

Figure S2c: Male : female distributions GEP and TMA cohorts

Figure S2d: Histology distributions GEP and TMA cohorts

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

All patients GEP All patients TMA All patients GEP + TMA

All infants GEP All infants TMA All infants GEP + TMA

All children GEP All children TMA All children GEP + TMA

All adults GEP All adults TMA All adults GEP + TMA

Figure S2e: Metastasis frequencies GEP and TMA cohorts

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

WNT SHH GRP3 GRP40%

50%

100%

All patients GEP All patients TMA All patients GEP + TMA

All infants GEP All infants TMA All infants GEP + TMA

All children GEP All children TMA All children GEP + TMA

All adults GEP All adults TMA All adults GEP + TMA

All patients Infants Children Adults

WNT SHH GROUP 3 GROUP 4

Figure S3: Survival analyses subgroups TMA

a b c d

e f g h

i j k l

m n o p

q r s t

P < 0.001 P < 0.001 P < 0.001

P = 0.001 NS P < 0.001

P < 0.001 P = 0.001 P < 0.001 P = 0.004

NS NS

P = 0.017

P < 0.001

P = 0.046

P = 0.009 NS

P = 0.004 P < 0.001

WNT WNT

WNT

SHH

SHH

SHH

GROUP 3 GROUP 3GROUP 3

GROUP 4GROUP 4

GROUP 4

SHH

M0M0

M0

M0

M+

M+ M+

M+

DESMOPLASTIC DESMOPLASTICDESMOPLASTIC

DESMOPLASTIC

CLASSIC

CLASSICCLASSIC

CLASSICLCA

LCA

LCALCA

M0M0

M0

M0

M+

M+

M+

M+

DESMOPLASTIC

CLASSIC

CLASSIC

CLASSIC

CLASSIC

LCA

LCA

LCA