XML Fundamentals Transparency No. 1 XML Fundamentals Cheng-Chia Chen.
Chen Cheng (ppt)
Transcript of Chen Cheng (ppt)
Control
SRYTarget
RNA
RNA Blot
Seed mRNA Flower mRNA
Primers SpecificFor bobg mRNA
Quantitative RT-PCR
Microarrays
Tools For Studying Differential Gene Activity
Eukaryotic RNAs Are Single-Stranded Polynucleotides
NucleotidesJoined By
Phosphodiester Bonds Like All Nucleic Acids
Order 5’ 3’ Leads to Function:
Co-Linear With Gene Sequence
Total RNA Isolation Workflow
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Collecting plant
materials (1-6)
Lysis (7-11)
(12-16)
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Washing (21-32)
Precipitation & Binding
(17-20)
Elute RNA (33-
40)Determining Concentratio
n
1. RNA Isolation
2. Remove Genomic DNA
RNase-Free DNase I
3. ElectrophoresisDetermining Quality
of Isolated RNA
Determining Quality of Isolated Total RNA Using A Capillary Gel Electrophoresis System
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Determining Quality of Isolated Total RNA Using A Capillary Gel Electrophoresis System
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