ChemiDoc XRS+ With Image Lab

8/14/2019 ChemiDoc XRS+ With Image Lab

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Imaging

Effortless and Accurate Imaging

and Analysis for Gels and Blots

Molecular ImagerChemiDocXRS+Systems


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Solve Complex Biological Questionswith Simple and Reliable Imaging

For more than two decades, Molecular Imager systems

rom Bio-Rad have been widely recognized and trusted

high-quality imaging instruments. Whether your research

includes routine imaging o chemiluminescent western

blots rom cell lysates or puriied proteins or SDS-PAGE

or PCR products or diagnostics or therapeutic

development the ChemiDoc XRS+ imaging system will

meet your needs. Designed or ease o use and ability to

support a wide array o applications, it is a perect it or

individual laboratories or multiuser core acilities.

Quality standards o engineering and manuacturing

make the system adaptable to academic or

biopharmaceutical laboratories. Thousands oresearchers over the years have published images

acquired with the ChemiDoc XRS system in journal

articles and grant proposals and established a record o

reliability and excellence or the system.

The Molecular Imager ChemiDoc XRS+ system enables

direct digital visualization o chemiluminescent western

blots or accurate images o accumulated signal rom

the chemiluminescent reaction. It provides reliable

quantitative data or characterizing your samples

reproducibly.

The ChemiDoc XRS+ system is lexible and easy to

use and supports other detection methods includingluorescence and colorimetry. It is the ideal complement

to your electrophoresis, puriication, and PCR systems,

enabling image analysis and documentation o western

blots, protein and DNA gels, and other sample types.

What the ChemiDoc XRS+ System

Can Do for You:

Automated workflow from image to results

n Makes your blot and gel imaging and analysis quick

and eortless

n Eliminates the need or training

n Allows any user to repeat the same worklow in

exactly the same way

Automated image capture

n Produces beautiul pictures o your blots and gels

n Delivers quantitative analysis o your protein and

DNA samplesAutomated image optimization

n Delivers image data that is always optimized

and reproducible without imaging artiacts

n Eliminates the need to use costly and

undependable X-ray ilm techniques

Automated image analysis

n Produces reports with data organized

the way you want

n Minimizes time rom lab work

to presentations


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Immun-StarWestern

chemiluminescent dete

SYBR Safe stain

Coomassie Brilliant BR-250 stain

Oriole stain

Qdot blot

ChemiDoc XRS+ System Applications

Nucleic Acid Protein Gel

Electrophoresis Electrophoresis Blotting

Ethidium bromide Coomassie Blue Chemiluminescent

SYBR Green Copper stain Colorimetric

SYBR Sae Zinc stain Qdots 525

SYBR Gold Flamingo uorescent gel stain Qdots 565

GelGreen Oriole stain Qdots 625

GelRed Silver stain CY2

Fast Blast DNA stain Coomassie Fluor Orange Alexa Fluor 488

SYPRO Ruby DyLight 488

Krypton Fluorescein


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Fast Results from a Completely

Automated Workflow

The ChemiDoc XRS+ system is controlled by Image Lab

sotware to automatically and reproducibly generate blot

and gel images. Image Lab sotware is ast, taking you

rom blot to printed results in seconds. Your results are

visible with a single click o the computer mouse.

Image Lab sotware eliminates the guesswork in imaging.

You wont have to perorm tedious repetitive steps to ind

the right ocus setting. You wont have to guess which

exposure times will best visualize the bands o interest.

Image calculations and corrections are done automatically

or your application. Whether you are working with protein

gels, nucleic acid gels, blots, or your own customized

imaging application, the ChemiDoc XRS+ system will select

the proper settings or optimum detection conditions o the

stain, label, or light-emitting substrate in use.

You wont need to set aside time or training new users;

the system and the sotware are easy to use and work

together rom setup to inish. Imaging and image analysis

can become the simplest work in the laboratory.

ChemiDoc XRS+ System Is Poweredwith Image LabSotware

Simple and Reproducible Image Capture

Place your gel or blot on the ChemiDoc imagers sample tray

and run your protocol: Your work is done in just one step.

Design and save protocols or the imaging steps in your

experiments, and Image Lab sotware will run the protocols

exactly the same way every time. The protocol eature negatvariability in results due to dierent people operating the

imaging system. When a new project is related to an existing

one, you can reuse the previously created protocol by revisin

its parameters and renaming it. Image Lab sotware lets you

devote your valuable time to research and discovery instead

to wondering i you used your imaging system properly.

Lane proles depict band intensity and represent quantities o sample

components separated in a gel.

Automated workfow or any application.


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Results and ReportsIn addition to printing a picture o your gel or blot or your

records, Image Lab sotware creates and prints reports o

your experimental data. Any part o the report can be copied

into popular document processing applications such as

Adobe Acrobat and Microsot Word, PowerPoint, or Excel

iles. To include a 3-D view o your gel or blot, copy it using

the Snapshot tool, and paste it into your presentation slide.

High quality, good-looking reports are easy to produce with

the combined power o the ChemiDoc XRS+ system and

Image Lab sotware.

When an analysis parameter is changed, the results tables

are updated instantly to relect the new data.

Tutorials

With Image Lab sotware, you dont need previous imaging

experience to produce optimal gel and blot images. Detailed

tutorials are accessible via the toolbar and start-up page to

acquaint you with all o the Image Lab sotware capabilities.

Three Unique Features

The power o Image Lab sotware stems rom

three eatures:

n

Image Lab sotwares proprietary algorithms (patentpending) calibrate the system at setup or automatic

control o ocus at any zoom level and automatic

correction o imaging artiacts

n Image Lab sotware perorms lat ielding corrections

speciically and consistently or each application

n Image Lab sotware saves the entire worklow (image capture,

results, report) in a protocol ile. The protocols can be edited,

resaved, reused, and shared among multiple users

Tutorials or novice users.

Its that easy to use Image Lab to generate the data andreports that you need. Please look at the other tutorials ormore detailed explanations o other Image Lab eatures.

Immunodetection

Chemiluminescent Detection

Use the signal accumulation mode (SAM) o the

ChemiDoc XRS+ system to record the time course o

development o the chemiluminescent reaction.

n Eliminate the guesswork oten involved with single-

event ilm capture o blot signals

n Simpliy chemiluminescent digital imaging

During the live acquisition, the ChemiDoc XRS+

system records progressive image development, allowi

users to:

n Visualize the accumulated chemiluminescent signal at

multiple time points and capture the image

n Customize acquisition time and the number o

images taken

n Save and analyze each image while the next image is

being captured, even beore the series is complete

Automatic digital capture o chemiluminescent blot signal

eicient.

n Eliminate the chemical use and disposal problems

involved with ilm developing

n The sel-contained lighttight enclosure means you dont

need a separate darkroom

For a more detailed comparison o digital detection with t

ChemiDoc XRS+ system and analog detection with ilm,

reer to bulletin 5809.

Comparison o chemiluminescent western blot detection on X-ray lm

and with the ChemiDoc XRS+ system. Blots o twoold serial dilutions

o human serum were probed with rabbit anti-human transerrin polyclonal

antibodies. A 1/1,000 dilution o human serum was used to make the twoo

serial dilutions. A 300 sec exposure on flm does not reach the same limit o

detection that is reached by a 60 sec exposure in the ChemiDoc XRS+ sys

X-ray lm (300 sec exposure)

ChemiDoc XRS+ system (60 sec exposure)

Human serum volume, pL

500 250 125 62.5 31.25 15.62 7.81 3.9 1.95

Chemiluminescence,

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Limit o Detection o Film and ChemiDoc XRS+ System

300,000

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X-ray flm

ChemiDoc XRS+ syst


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Quantitative dynamic range and visualization tools.A, the ChemiDoc XRS+

system detects a wide range o sample concentrations without saturating the

most concentrated band, enabling linear quantitation. A variety o visualization

tools are provided or sample verifcation. B, the 3-D viewer shows the distinct

peaks o the most concentrated band as well as the aintest band. C, the lane

profles provide quick comparison o expression levels rom each lane. D, the

volume regression curve shows a correlation coefcient R2 o 0.986909

confrming linear data over the entire range o sample detection.

A C

B D

Chemiluminescent ELISA arrays.The

Quansys Biosciences Q-Plex array has16 distinct capture antibodies bound to

each well o a 96-well plate. The attributes

o high resolution and sensitivity enable

the ChemiDoc XRS+ system to image the

entire plate (panelA)while resolving each

array spot (panel B) with the dynamic

range (panel C) to provide quantitative

data (panel D).

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C

D

Accurate Quantitation Visualized

The ChemiDoc XRS+ system can perorm accurate

quantitative detection with high resolution and sensitivit y

on a wide variety o samples.

Chemiluminescent sample detection and visible markers.Visible markers

are commonly used to monitor problems with SDS-PAGE and blot transer. To

determine the molecular weights, users trace the pattern o the visible marker

rom the blot to flm. This adds additional steps and can possibly introduce

errors. The ChemiDoc XRS+ system images the chemiluminescent samples

and visible markers to provide a digital record; the images are then merged or

molecular weight estimation.

Chemiluminescence

Chemiluminescence image rom

sample merged with visible marker

Chemiluminescent and Colorimetric Detection

Combined in a Single Blot

Multifuorescent western blot detection o maltose binding protein

(MBP) and Pronity eXact usion-tagged MBP bands using Qdot 625

and Qdot 525 fuorescent conjugated secondary antibodies (Invitrogen

Corp.), respectively, imaged with the ChemiDoc XRS+ system.The

blot was imaged with excitation via UV, and separate images were taken by

switching between 520/30 (Qdot 525) and 630/30 (Qdot 625) flters installedin the ChemiDoc XRS+ imager. Multiuorescent western blotting enables

detection o two antibody-specifc proteins at once, removing the variability

involved with two blots or the loss o protein with stripping. For more

inormation, reer to bulletin 5792.

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750 370 200

100 40 30 15 6.0 3.0 1.5

Multifluorescent Western Blot

Visible marker


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Veriication and Quantitative Analysis

Molecular Biology

Gel electrophoresis remains an important tool or

quantitative analysis o complex protein and nucleic acid

samples. Applications include nucleic acid isolation or

ampliication or molecular cloning techniques, nucleic

acid quality evaluation prior to quantitative PCR, gene

silencing, gene modulation, and gene expression.

ChemiDoc XRS+ systems are suitable or laboratories

engaged in RNAi analysis, molecular diagnostics,

epigenetics, pharmacogenomics, and orensic testing o

genomic DNA.

Protein gels stained with Coomassie Blue stain (let) and silver stain

(right). Documentation o protein gels or lab notebooks or sample analysis,

including protein purity and concentration assessment or dierential protein

expression studies or to monitor gene modulation products, is suppor ted by

the array o analysis tools in the ChemiDoc XRS+ system. The gel stained

with silver stain shows salmon muscle, soybean, and rat brain extracts,

protein mixtures, and E. coliextracts compared with Precision Plus Protein

unstained standards.

Protein gels fuorescence-stained with Oriole stain (let) and Flamingo

fuorescent gel stain (right).Achieve higher levels o sensitivity with

uorescent stains to dierentiate proteins with low expression. Resolve

closely spaced spots or bands or protein profling, quantitation, and

characterization.

Protein Analysis

ChemiDoc XRS+ systems are oten incorporated into

sample preparation and sample quantitation worklows.

An alternative to UV illumination to better preserve DNA samples.

Top, serial dilutions o EtBr-stained precision molecular mass ruler

(Bio-Rad Laboratories, Inc.) on agarose gel imaged with UV light; bottom,

serially diluted precision molecular mass ruler stained with SYBR Sae

stain on agarose gel imaged with XcitaBlueconversion screen.There

is no loss in sensitivity when a combination o SYBR Sae nucleic acid

uorescent stain and less harmul blue excitation is used instead o UV-

excitable EtBr. The image o the gel s tained with SYBR Sae stain was

taken using the XcitaBlue conversion screen and a flter or SYBR Sae

stain and GFP. Less harmul detection methods better preserve samples

or downstream uses such as cloning.

Ethidium Bromide and SYBR Safe DNA Stain Detection

Fluorescence,

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Sample load, ng

51.2 25.6 12.8 6.4 3.2 1.62 0.8 0.4 0.2 0.1

SYBR Sae andXcitaBlue conversion

screen

EtBr and UV light


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Life ScienceGroup

09-0690 1009 Sig 0308Bulletin 5837 Rev B US/EG

Bio-RadLaboratories, Inc.

Web site www.bio-rad.com USA 800 4BIORAD Australia 61 02 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil55 21 3237 9400Canada 905 364 3435 China 86 21 6426 0808 Czech Republic420 241 430 532 Denmark44 52 10 00 Finland09 804 22 00 France 01 47 95 69 65Germany089 318 84 0 Greece 30 210 777 4396 Hong Kong 852 2789 3300 Hungary36 1 455 8800 India 91 124 4029300 Israel03 963 6050Italy39 02 216091 Japan 03 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Netherlands 0318 540666 New Zealand0508 805 500Norway23 38 41 30 Poland48 22 331 99 99 Portugal351 21 472 7700 Russia 7 495 721 14 04 Singapore 65 6415 3188 South Africa 27 861 246 723Spain 34 91 590 5200 Sweden 08 555 12700 Switzerland061 717 95 55 Taiwan 886 2 2578 7189 United Kingdom 020 8328 2000

Specifications

Automation Capabilities

Workow automated selection Application driven; user selected or recalled by a protocol

Workow automated execution Controlled by a protocol via application specifc setup or image area, illumination source, flter,

analysis, and reporting

Workow reproducibili ty 100% repeatabil ity via recallable protocols; rom image capture to quantitative analysis and reports

Autoocus (patent pending) Precalibrated ocus or any zoom setting or sample height

Image at felding* Dynamic; precalibrated and optimized or every application

Autoexposure 2 user-defned modes (intense or aint bands)

Hardware Specications

Maximum sample size 28 x 36 cm

Maximum image area 25 x 26 cm

Excitation source Trans-UV and epi-white are standard (302 nm included, with 254 nm and 365 nm available as options);

optional trans white, sel-powered or conversion screen; optional XcitaBlue UV/blue conversion screen

Illumination control 5 modes (trans-UV, epi-white, and no illumination or chemiluminescence are standard); trans white

and XcitaBlue conversion screens are options

Detector Supercooled CCD

CCD resolution (H x V) 1,392 x 1,040 pixels

Pixel size (H x V) 6.45 x 6.45 m

Cooling system Peltier

Camera cooling temperature 30C controlled

Filter selector 3 positions (2 or flters, 1 without flter, or chemiluminescence)

Emission flters 1 included (standard), 3 optional

Dynamic range >4.0 orders o magnitude

Pixel density (gray levels) 65,535Instrument size (L x W x H) 36 x 60 x 96 cm

Instrument weight 32 kg

Operating Ranges

Operating voltage 110/115/230 V AC nominal

Operating temperature 1028C (21C recommended)

Operating humidity

ChemiDoc XRS+ With Image Lab

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