Charlie Drewes Biology Inquiry with Invertebrates Ecology, Evolution & Organismal Biology Iowa State...
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Transcript of Charlie Drewes Biology Inquiry with Invertebrates Ecology, Evolution & Organismal Biology Iowa State...
Charlie Drewes
Biology Inquiry Biology Inquiry with Invertebrateswith Invertebrates
Ecology, Evolution &
Organismal BiologyIowa State University
Ames, IA 50011
►► Guided inquiry “BIOLOGY in ACTION”
►► Open-ended inquiry”Stuck-on Artemia”
►► Independent Inquiry“Biological Smoke Detectors”
‘‘BIOLOGYBIOLOGY inin ACTION’ACTION’
31 Interactive & Computational
Animations
WEB-based and CD (non-proprietary)
INTERDISCIPLINARY AREAS:Biology, Math, Physics
TOPICS & FOCAL POINTS:
Locomotion BiomechanicsCell Biology Animal Behavior
Physiology DevelopmentInvertebrate Biology
adjuncts to hands-on laboratory inquiries with living organisms
adjuncts to lecture / discussion (homework or student presentations)
focal points for problem-based learning (computation & data analysis)
CURRICULAR UTILITY:
RED = interactive animations with computations and control features
BLACK = GIF animations with questions and links
LINK to CONTENTS
►
►
►
Hands-on Lab Investigations with Living Lumbriculus
Circulatory Function (ABT) (blood flow, pulse rate) Locomotion & Movement (ABT)
(swimming, crawling, reversal behavior)
Segment Regeneration (ABLE) (development; asexual reproduction)
Interactive format
Animations of Lumbriculus blood vessel pulsations: Adjunct to lab inquiry of circulation in living worms
0 sec0 sec
2 sec2 sec
3 sec3 sec
4 sec4 sec
5 sec5 sec
1 sec1 sec
CirculatoryComputations
pulse frequency
(pulse waves /sec) pulse velocity (mm /sec)
blood volume (cc3 / time)
TAIL TOUCH
Animation of helical swimming in Lumbriculus:
A follow-up to student lab inquiry of locomotion
in living worms
Helical swimming in Lumbriculus:
A unique form of animal locomotion
elapsed time (f1elapsed time (f1f7) = f7) = 0.2 sec0.2 sec
directionof swimming
direction ofwave & backthrust
forward velocity = ?
wave frequency = ?
wave velocity = ?
Reynolds number = ?
The Formation of The Formation of Vegetable Mould, through Vegetable Mould, through
the Action of Worms,the Action of Worms, with Observations on Their Habitswith Observations on Their Habits
by by
Charles DarwinCharles Darwin
18811881
Animation of peristaltic crawling…
…a follow-up to student lab inquiry about hydrostatic skeletons & locomotion
without appendages in living invertebrates
(e.g., terrestrial and aquatic annelid worms)
ELONGATED SEGMENT:
length = 17.5 mm; diameter = 13 mmvolume = π · r2 · length = 2,321 mm3
SHORTENED SEGMENT:
length = 7.5 mm; diameter = 20 mmvolume = π · r2 · length = 2,355 mm3
I
I
plastic petri dishtransparency strip with grid
10 mm x 10 mm
strip of double-stick tape covers grid
tape holds strip to dish
STUCK-on ARTEMIATechnical Advantages
easy handling & transfer of cysts
uses small, quantifiable number of cysts
follow development of individual cysts
cysts stay in single focal plane
use dissecting or compound microscope
manipulation of many relevant variables
Hatching of encystedArtemia embryos
Encysted late gastrula stage
Cyst hydrates and swells
Cyst wall splits
Membrane-bound“umbrella” stage
Nauplius larva emergence
Experimental variablesPHYSICAL & CHEMICAL ENVIRONMENT
Temperature (heating, cooling, freezing)Sun light (UV)Microwave irradiationSalinity (hypo- & hyperosmotic)Repeated hydrationDissolved oxygenToxicants (pollutants, pharmacological agents)
Measurements & End-pointsHatching % Hatching time-courseDevelopmental anomalies
Larval motility
Molting & growth
1) Invertebrates as toxicity test organisms2) Selection of test chemical(s)3) Exposure / treatment protocols4) End-points (lethal versus sublethal effects)5) Concentration range-finding (preliminary expts)6) Final testing and analysis7) Materials, supplies, resource references8) SAFETY ISSUES & GLOSSARY
CONCEPTS & PRACTICAL IDEAS (non-cookbook)
InvertebratesInvertebrates are more than…
…taxonomic survey material
…anatomy & dissection material
…creepy, crawly novelties
Use of living Use of living invertebrates for invertebrates for
biology lab inquiries…biology lab inquiries…
… makes biology more than a ‘spectator sport’
… engages & enlivens interest
… promotes analytical thinking
Take-Home MaterialsTake-Home Materials
CD:CD: ““BIOLOGY in ACTION”BIOLOGY in ACTION” - - 3131 animationsanimations & “Freshwater Invertebrates” - PowerPt- PowerPt filefile
Copy:Copy: ContentsContents & Animation& Animation Q/A’s for InstructorQ/A’s for Instructor Copy: Copy: Student lab inquiry: “ Stuck-on Artemia” Stuck-on Artemia” Reprint:Reprint: “Biological Smoke Detectors”