Chapter 7 · What is DNA? Section 7.1 DNA stored information for protein production. DNA (genotype)...
Transcript of Chapter 7 · What is DNA? Section 7.1 DNA stored information for protein production. DNA (genotype)...
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Chapter 7 DNA Structure and Gene
Function
DNA bursting from bacterial cell © Dr. Gopal Murti/Science Source
Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education.
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What is DNA?
Section 7.1
DNA stored information for protein production.
DNA (genotype)
RNA
Protein (phenotype)
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Rosalind Franklin
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Watson & Crick
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Figure 17.2
Double-stranded DNA
Histones
Chromatinmaterial:not visibleduringInterphase
One chromatid
Its sisterchromatid
Centromere
Chromosome: visible during mitosis
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DNA Review
Base pair
Phosphate
Sugar
Nucleotide
Figure 2.24
• DNA Nucleotide
• Deoxyribose sugar
• Phosphate group
• Nitrogenous base
• A, G, C, T
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DNA Structure
Sugars &
phosphates
Nitrogenous Bases
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DNA Base Pair Rules(Chargaff’s Rules)
DNA Base Pairing
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Section 7.1 Figure 7.5
Hydrogen bonds connect complementary DNA strands.
Hydrogen bonds
DNA Is a Double Helix
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RNA Review
• RNA Nucleotide
• Ribose sugar
• Phosphate group
• Nitrogenous base
• A, G, C, U
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Review: DNA & RNA Comparison
Section 7.3 Figure 7.9
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Nitrogenous Bases
1. Which nitrogenous base is only found in RNA?
uracil
2. Which nitrogenous base is only found in DNA?
thymine
3. Which nitrogenous bases are found in both DNA & RNA?
adenine/guanine/cytosine
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What is the main function of DNA?
A. encode proteins B. produce ATP C. speed up cell reactionsD. provide structural support to the cellE. All of the choices are correct.
Flower: © Doug Sherman/Geofile/RF
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• Prokaryotic cells• Transcription
DNA RNA
• Translation
RNA proteins
Protein Synthesis
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Protein Synthesis
• Eukaryotic cells• Transcription
DNA RNA
• RNA Processing
Modify pre-mRNA
• Translation
RNA proteins
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Genetic Code
• Codon• mRNA
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Fig. 17-6
(a) Tobacco plant expressing
a firefly gene
(b) Pig expressing a
jellyfish gene
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Protein Production Starts with DNA
Section 7.3 Figure 7.8
Transcription: DNA RNA• 3 types of RNA
Translation: RNA Protein
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Fig. 17-1
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Protein Production Starts with DNA
Section 7.3 Figure 7.8
Protein analogy Cooking
Modification to ingredients?
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Transcription Uses DNA to Create RNA
Section 7.4 Figures 7.8, 7.9
How does DNA pair with RNA?
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Fig. 17-7
Promoter Transcription unit
Start pointDNA
RNA polymerase
5533
Initiation1
2
3
5533
Unwound
DNA
RNAtranscript
Template strand
of DNA
Elongation
Rewound
DNA
5
55
5
5
33
3
3
RNA
transcriptTermination
5533
35Completed RNA transcript
Newly made
RNA
Template
strand of DNA
Direction oftranscription(“downstream”)
3 end
RNA
polymerase
RNA nucleotides
Nontemplate
strand of DNAElongation
Transcription Overview
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Transcription Uses DNA to Create RNA
RNA polymerase binds to the promoter region
DNA
Initiation
RNA polymerase enzyme
Promoter DNA template strand
Initiation
Figure 7.10Section 7.4
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Transcription Uses DNA to Create RNA
RNA complementary to DNAElongation
RNA polymerase
RNA
DNA
Elongation
Section 7.4
3’
5’
5’
5’3’
3’
Figure 7.10
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Transcription Uses DNA to Create RNA
Termination
RNA polymerase
DNA
TerminatorRNA
Termination
Figure 7.10Section 7.4
RNA, DNA, and RNA polymerase separate.
DNA becomes a double helix again.
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Transcription Uses DNA to Create RNA
The cell produced an RNA copy of a gene!
Termination
RNA polymerase
DNA
TerminatorRNA
Termination
Figure 7.10Section 7.4
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Fig. 17-7
Promoter Transcription unit
Start pointDNA
RNA polymerase
5533
Initiation1
2
3
5533
Unwound
DNA
RNAtranscript
Template strand
of DNA
Elongation
Rewound
DNA
5
55
5
5
33
3
3
RNA
transcriptTermination
5533
35Completed RNA transcript
Newly made
RNA
Template
strand of DNA
Direction oftranscription(“downstream”)
3 end
RNA
polymerase
RNA nucleotides
Nontemplate
strand of DNAElongation
Transcription Overview
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If DNA reads 5' - TACTTCAAAATC - 3‘
• What are the transcribed RNA bases?
• How many codons?
• How many amino acids will be present
after translation?
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RNA Is Processed in the Nucleus
Figure 7.11
Poly A tail and mRNA cap are added to the RNA.
Section 7.4
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RNA Is Processed in the Nucleus
Introns are removed from the RNA molecule.
Introns
Exons
Figure 7.11Section 7.4
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RNA Is Processed in the Nucleus
The RNA then leaves the nucleus. Onward to
translation!
Figure 7.11Section 7.4
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Fig. 18-11
or
RNA splicing
mRNA
PrimaryRNAtranscript
Troponin T gene
Exons
DNA
Alternative Splicing
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If the DNA template strand has the following sequence, what would be the nucleotide sequence of the complementary RNA molecule produced in transcription?
Template strand: AGTCTT
A. AGTCTTB. AGUCUUC. TCAGAAD. TCUGUUE. UCAGAA
Flower: © Doug Sherman/Geofile/RF
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7.4 Mastering Concepts
How is mRNA modified before it leaves the nucleus of a eukaryotic cell?
DNA bursting from bacterial cell © Dr. Gopal Murti/Science Source
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Translation Builds the Protein
Section 7.5 Figure 7.8
Now let’s look at how a ribosome uses RNA to produce a protein.
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Translation Builds the Protein
Figure 7.12
A
A A
A AG
G G
G UU C
CTT T C
C
DNA template strandDNA
TRANSCRIPTION
mRNA
TRANSLATION
Protein
CodonCodonCodon
Lysine ValineSerine
Polypeptide (amino acid sequence)
A codon is a three-nucleotide sequence that encodes one amino acid.
Section 7.5
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Translation Builds the Protein
U
C
A
G
U C A G
Firs
t le
tter
of
cod
on
U
C
A
G
U
C
A
G
U
C
A
G
U
C
A
G
Third
letter of co
do
nA A AG G UU CC
mRNA
TRANSLATION
Protein
CodonCodonCodon
Lysine ValineSerine
Polypeptide (amino acid sequence)
The Genetic Code
Second letter of codon
UUU
UUC
UUA
UUG
CUU
CUC
CUA
CUG
AUU
AUC
AUA
AUG
GUU
GUC
GUA
GUG
UAU
UAC
CCA
CCG
UAA
UCU
UCC
UCA
UCG
CCU
CCC
UAG
UGU
UGC
UGA
UGG
CAU
CAC
CAA
CAG
CGU
CGC
CGA
CGG
ACU
ACC
ACA
ACG
AAU
AAC
AAA
AAG
AGU
AGC
AGA
AGG
GCU
GCC
GCA
GCG
GAU
GAC
GAA
GAG
GGU
GGC
GGA
GGG
Leucine (Leu; L)
Phenylalanine (Phe; F)
Leucine (Leu; L)
Isoleucine (Ile; I)
Start Methionine (Met; M)
Valine (Val; V)
Serine (Ser; S)
Proline (Pro; P)
Proline (Pro; P)
Proline (Pro; P)
Tyrosine (Tyr; Y)
Histidine (His; H)
Glutamine (Gln; Q)
Asparagine (Asn; N)
Lysine (Lys; K)
Aspartic acid (Asp; D)
Glutamic acid (Glu; E)
Cysteine (Cys; C)
Tryptophan (Trp; W)
Stop
Arginine (Arg; R)
Serine (Ser; S)
Arginine (Arg; R)
Glysine (Gly; G)
Stop
Stop
The genetic code shows which mRNA codons correspond to which amino acids.
Section 7.5 Figure 7.12
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Translation
Cast members of Protein Synthesis
• mRNA
Translation Builds the Protein
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Transfer RNA (tRNA) translate the genetic code.
tRNA: © Tom Pantages/PhototakeSection 7.5 Figure 7.13
Translation
Cast members of Protein Synthesis
Translation Builds the Protein
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• Ribosome• Large subunit
P site
A site
• Small subunit
Translation
Cast members of Protein Synthesis
Translation Builds the Protein
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Translation
Protein Synthesis – Initiation
1. Binding of mRNA, 1st tRNA w/ aa and small ribosome
2. Binding of the large subunit
Translation Builds the Protein
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Translation
Protein Synthesis
Elongation• Codon recognition
Type of bond?
• Peptide bond formation
• tRNA in P-site leaves
• tRNA in A-site has
protein
• translocation
Translation Builds the Protein
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Translation
Protein Synthesis – Termination
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Polyribosomes
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Translation Builds the Protein
PolypeptidemRNA Ribosome
SEM (false color) 50 nm
Ribosomes: © Kiseleva and Donald Fawcett/Visuals Unlimited
Translation is efficient when multiple ribosomes attach to an mRNA molecule simultaneously.
Section 7.5 Figure 7.16
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Fig. 17-21
Ribosome
mRNA
Signalpeptide
Signal-recognitionparticle (SRP)
CYTOSOLTranslocationcomplex
SRPreceptorprotein
ER LUMEN
Signalpeptideremoved
ERmembrane
Protein
Secretory proteins – endomembrane system
Protein Synthesis
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• Amino acid
• tRNA UAC anticodon
• mRNA codon
• DNA
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Mutations Change DNA
A mutation is a change in a cell’s DNA sequence. Mutations come in several varieties.
Section 7.7 Figure 7.20Wild fly: © Andrew Syred/Science Source; Mutant fly: © Science VU/Dr. F. R. Turner/Visuals Unlimited
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Mutations Change DNA
A point mutation changes one or a few base pairs in a gene.
Table 7.2Section 7.7
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Normal cells: © Micro Discovery/Corbis; Sickled cells: © Dr. Gopal Murti/Science Source
Normal red blood cells
No aggregation
of hemoglobin
molecules
SEM
Pro Glu Glu
6 µm
Sickled red blood cells
Abnormal
aggregation
of hemoglobin
molecules
Pro Val Glu6 µm
G G A C T C C T T
C C U G A G G A A
G G A C A C C T T
C C U G U G G A A
SEM
Point Mutation -Substitution
Figure 7.21
Mutations Change DNA
Section 7.7
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Mutations Change DNA
Wildtype = original nucleotide sequence
Substitution = changed nucleotide(s)
In lab 1 base changeSalt instead of sugar
Silent mutation?
Table 7.2Section 7.7
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Mutations Change DNA
“Frameshift”mutations affect multiple codons.
Insertion of one nucleotide changes every codon after the insertion.
Table 7.2Section 7.7
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Figure 10.16B
Normalgene
Nucleotidesubstitution
Nucleotidedeletion
Nucleotideinsertion
Inserted
Deleted
mRNA
Protein Met
Met
Lys Phe
Lys Phe
Ala
Ala
Gly
Ser
A U G A A G U U U G G C G C A
G C G C AAG U U UA U G A A
Met Lys Ala HisLeu
G U UA U G A A G G C G C A U
U
Met Lys Ala HisLeu
G U UA U G A A G G CU G G C
Frameshift Mutations
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Mutations Change DNA
Figure 7.22Section 7.7
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But mutations are not always harmful!
• Mutations create different versions of genesAlleles alternative versions of the same gene
• Genetic variation is important for evolution.
• What determines if a mutation is advantageous or not?
Mutations Change DNA
Section 7.7 Figure 7.23Grapefruit: © Erich Schlegel/Dallas Morning News/Corbis; rice: © Pallava Bagla/Corbis; cotton: © Scott
Olson/Getty Images
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Chapter 8 DNA Replication, Binary Fission,
and Mitosis
World’s tallest man © Frederic J. Brown/AFP/Getty Images
Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education.
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Two Types of Cell Division Interact in the Sexual Life Cycle
Section 8.1
Sex cells combine at fertilization.
Figure 8.1
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Mitosis Has Many Roles
Section 8.1
1) Grow and develop2) repair tissues3) regenerate lost body parts4) Some organisms reproduce asexually by mitosis
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Mitosis Has Many Roles
Section 8.1 Figure 8.2
Mitosis produces the cells that build the human body.
Day 1 zygote: © Pascal Goetgheluck/Science SourceDay 2, 3 zygote: © Richard G. Rawlins, Ph.D./ Custom Medical Stock PhotoMiddle and bottom row photos: Bradley R. Smith, Ph.D.
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Cell Death Is Part of Life
Section 8.1
Apoptosis, or cell death, carves out distinctive structures.
Figure 8.3Duckling: © GK hart/Vikki Hart/Getty Images RF
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Mitosis and Apoptosis Work Together
Section 8.1
Mitosis adds new cells while apoptosis removes them, allowing tissues to renew themselves.
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Shortly after fertilization, a zygote divides into two identical cells. What type of cell division produces these two cells?
A. mitotic cell divisionB. meiotic cell divisionC. apoptosis
Flower: © Doug Sherman/Geofile/RF
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DNA Replication Precedes Cell Division
Section 8.2
For each of the daughter cells from this division to have identical DNA, the cell must first replicate its genome, all of the cell’s genetic material.
Tumor cells: © Steve Gschmeissner/SPL/Getty Images RF
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Figure 17.1
Mitosis
Nucleus divides
Cytokinesis
Cell divides
Cell prepares for division.Growth continuesslowly.
Primary period
of cell growth.
G2G1
S
DNA is duplicated.Growth continuesslowly.
M
G0
Cell Cycle
• Interphase
• Mitotic phase
(karyokinesis)
– Prophase
– Metaphase
– Anaphase
– Telophase
• Cytokinesis
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DNA Replication
• When does DNA
replication take place?
• Why does DNA
replication take place?
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DNA Replication Overview
Centromere
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Figure 17.4
Keys:
= Cytosine
= Adenine
= Guanine
= Thymine
Replication
bubble
Parent DNA
molecule
2 complete
daughter DNA
molecules
Parent
strands
New
complementary
strands
b) The unwinding and the formation of new strands occur
simultaneously at many sites on the DNA molecule.
The sites of replication expand outward until they join. For
simplicity the two strands are shown as parallel in (b),
but in actuality they form a helical shape as shown in (a).
Parent strand New
(daughter)
strands
forming
Parent strand
a) The double-stranded DNA unwinds, and each single
strand serves as a template for a new complementary
strand.
Why many origins of replication?
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DNA Replication Enzymes
1. Helicase
2. Single strand binding protein
3. Primase
4. DNA polymerase
5. DNA ligase
1. Looks for start points
Opens DNA
2. Helps keep DNA opened
3. Primes DNA replication
10 RNA nucleotides
4. a) Copies DNA
b) converts RNA nucleotides to DNA nucleotides
c) proofreads
d) corrects errors
5. Joins DNA sections (fragments)
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DNA Replication Precedes Cell Division
Section 8.2
Semi conservative:½ old
&½ new
Figure 8.6
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DNA Replication Precedes Cell Division
Section 8.2
1) Helicases 2) Binding proteins
Figure 8.5
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DNA Replication Precedes Cell Division
Section 8.2
3) Primase
Figure 8.5
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Priming for DNA Replication
• Primase RNA primer
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DNA Replication Precedes Cell Division
Section 8.2
4) DNA polymerase
New strand 5’ 3’
Figure 8.5
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DNA Elongation
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Replication Fork
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DNA Replication Precedes Cell Division
Section 8.2
Leading Strand: synthesis is continuous.
Figure 8.5
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Leading & Lagging Strands
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DNA Replication Precedes Cell Division
Section 8.2
Lagging Strand: opposite direction from helicase movement. Strand synthesis is discontinuous.
Figure 8.5
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DNA Replication Precedes Cell Division
Section 8.2
4) DNA polymerase RNA primer with DNA
5) Ligases
Figure 8.5
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DNA Replication Precedes Cell Division
Section 8.2
Semi conservative:½ old
&½ new
Figure 8.6
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DNA Replication Enzymes
1. Helicase
2. Single strand binding protein
3. Primase
4. DNA polymerase
5. DNA ligase
1. Looks for start points
Opens DNA
2. Helps keep DNA opened
3. Primes DNA replication
10 RNA nucleotides
4. a) Copies DNA
b) converts RNA nucleotides to DNA nucleotides
c) proofreads
d) corrects errors
5. Joins DNA sections (fragments)
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Figure 10.22A
DNA enters
cell
A fragment ofDNA from anotherbacterial cell
Bacterial chromosome(DNA)
Prokaryotic Genetic Diversity
Horizontal Gene Transfer increases bacterial diversity
1. Transformation
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Figure 10.22B
Phage
A fragmentof DNA fromanotherbacterial cell(former phage host)
Horizontal Gene Transfer
2. Transduction
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Figure 10.22C
Mating bridge
Sex pili
Donor cell Recipient cell
Horizontal Gene Transfer
3. Conjugation
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Figure 10.22D
Donated DNA Crossovers Degraded DNA
Recombinantchromosome
Recipient cell’schromosome
Horizontal Gene Transfer
3. Conjugation
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Figure 10.20A
Envelope
Glycoprotein
Protein coat
RNA(two identicalstrands)
Reversetranscriptase(two copies)
HIV
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HIV & Protein Synthesis
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Figure 10.18_UN
Mumps Virus
•MMR vaccine (measles, mumps & rubella)
Polio
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2
Figure 10.18
Viral RNA (genome)
Glycoprotein spike
Protein coat
Membranousenvelope
Entry CYTOPLASM
Uncoating
Plasmamembraneof host cell
1
3
54
6
Proteinsynthesis
Viral RNA(genome)
RNA synthesisby viral enzyme
mRNA
Newviral proteins
Assembly
New viralgenome
Template
RNA synthesis(other strand)
Exit
7
6
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Figure 10.19
Emerging Viruses
How do they come arise?
•Mutations
•Contact btn species
•Spread from isolated
populations
HIV, West Nile Virus, SARS, H1N1 – swine flu
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Figure 10.UN03
DNA
(b)
is a polymermade from
monomers called
is performedby an
enzyme called(c)
(a)
(d)
(e)
(f)
comesin three
kinds called
use amino-acid-bearingmolecules called
is performedby structures
called (h)
molecules arecomponents of
RNA
Protein
(g)
(i)
one or more polymersmade from
monomers called