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Transcript of Chapter 7 Components of Optical Instruments. 8888 Components of optical instruments 1. Absorption 3....
![Page 1: Chapter 7 Components of Optical Instruments. 8888 Components of optical instruments 1. Absorption 3. Emission and chemiluminescence2. Fluorescence and.](https://reader033.fdocuments.us/reader033/viewer/2022061423/56649de45503460f94adba90/html5/thumbnails/1.jpg)
Chapter 7
Components of Optical Instruments
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8888
Components of optical instruments
1. Absorption3. Emission and chemiluminescence2. Fluorescence and phosphorescence
Source Wavelength Selector Sample Detector Readout
Rgb back 146,184. 148
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Source of Radiation
A source should be generate a beam of radiation with sufficient power
Its out put power should be stable for reasonable period
Continuum Sources (D2 lamp, Ar lamp, Xe lamp, Tungsten lamp)
Line Sources (Hollow cathode lamp, Hg vapor, Na vapor,
Electrodeless discharge lamp)
Laser Sources
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Wavelength 100 180 380 850 2000 18000 40000
Region VAC UV Visible Near IR IR Far IR
Sources
Continous
Line
Ar lamp
Xe lamp
H2 or D2 lamp
Tungtstan lamp
Nernst glower ZnO2+Y2O3
Nichrome wire
Glowbar SiC
Hollow cathode lamp
Lasers
Sources for Spectroscopic Instruments
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Light Amplification by Stimulated Emission of Radiation
LASER
• Characteristics of a laser:– Spatially narrow and intense– Highly monochromatic– Coherence
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Power supply
Pumping source
Radiation
Partially transmitting mirror
MirrorLaser radiation
Nonparallel radiation
Active lasing medium
Schematic of a Laser Source
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Ey’’’
Ey’’
Ey’
Ey
Ex
Metastable Excited state
1- Pumping
Excitation by electrical, radiant or chemical energy
1- Pumping
2- Spontaneous emission
1- Pumping
2- Spontaneous emission
3- Stimulated emission
1- Pumping
2- Spontaneous emission
3- Stimulated emission
4- Absorption
Processes in Laser Action
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Light attenuation by absorption
Noninverted population
Inverted population
Light amplification by stimulated emission
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Three level system Four level system
E0 E0
E1
Ey Ey
Ex
E1
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Wavelength Selectors
• Filters– Interference (UV-VIS)
– Absorption (VIS)
– Cut-off
• Monochromators – Gratings
– Prisms
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3000 lines/mm Grating 50 lines/mm
Wavelength selectors
Continous
Dis-continous
Fluorite prism
Fused silica or quartz prism
Glass prism
NaCl prism
Interference wedge
Interference filter
KBr prism
Glass filter
Region VAC UV Visible Near IR IR Far IR
Wavelength 100 180 380 850 2000 18000 40000
Wavelength Selectors for Spectroscopic Instruments
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Metal filmDielectric layer
Glass plate
Glass plate
Metal film
Interference Filters
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tDielectric layer
Metal film
Metal film
Condition for reinforcement n’ =2t/cos
If < 10o n’ =2t
= ’
tn
Interference Filters
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5090 5110 6215 6225 6940 6960
Effective bandwidth= 10
Effective bandwidth= 15 A
Effective bandwidth= 15 A
Effective bandwidth
1/2Peak
height
Wavelength
Per
cen
t T
ran
smit
tan
ce
100
80
60
40
20
0
Transmission Characteristic of Interference Filters
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Interference Filter
Effective bandwidth=10nm
Absorption Filter
Effective bandwidth= 50nm
40 450 500 550
100
60
40
20
0
80
Per
cen
t T
ran
smit
tan
ce
Effective Bandwidth of Filters
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400 500 600 700
100
50
0
Green filter
Orange cut-off filter
Combinationof two filters
Per
cen
t T
ran
smit
tan
ce
Wavelength nm
Coupling of Filters
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Monochromators
For many spectroscopic methods its necessary to be able to vary the wavelengthOf radiation that this process called scanning a spectrum. Monochromators were designed for spectral scanning.
Components of Monochromators1- Entrance slit2- Collimating lens or mirrors3- Dispersing element (prism or grating)4- Focusing element (lens)5- Exit slit
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Collimating lens
Focusing lensPrism
Exit slit
Entrance slit
Focal plane
Bunsen Prism Monochromator
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Reflection grating
Entrance slit Exit slit
Concave mirrors
Focal plane
Czerny Turner Grating Monochromator
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Gratings
1- Transmission Gratings
2- Reflection Gratings
• Replica Grating
• Echellette Grating
• Concave Grating
• Halographic Grating
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Replica Grating
Replica grating are manufactured from a MASTER grating which consists Of a hard, optically flat, polished surface upon which have been ruled with
A suitable shaped diamond tool a large number of parallel and closely spacedGrooves.
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n=1
Sourc
eDetector
d
d*sin rd*sin i
d* sin i + d sin r =
Monochromatic beams at incident angle i
Diffracted beams at refelected angle r
r
i
Echellette Grating
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d*sin r
d*sin i
Detector
d*sin rd*sin i
d
d* sin i + d *sin r = 1.5 *
Monochromatic beams at incident angle i
Diffracted beams at refelected angle r
r
i
Sourc
e
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Plate
Photoresist
UV lamp
Mask
Developing solution
Etching solution
Photolithography
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i
r
r = i = 63o26’n= 2dsini
Conventional Echelle Focal length 0.5m 0.5m Groove density 1200/mm 79/mm Diffraction angle 10o22’ 63o26’ Order n (at 300 nm) 1 75 Resolution (at 300 nm) 62400 763000 Recirocal linear disperdion 16 A/mm 1.5 A/mm Light gathering power f/9.8 f/8.8
Echelle Monochromator
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Performance Characteristics of Grating Monochromators
Quality of monchromators depend on:1- The purity of its radiant output. Stray Radiation2- The ability to resolve adjacent wavelength R = 3- The light gathering power f = F/d4- The spectral band width Bandwidth is defined as the span of monochromator settings (in units of wavelength) needed to move the image of the entrance slit across the exit slit.
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Detector
Wavelength
Pow
er Exit slit
Illumination of an Exit Slit
Monochromator
2
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Wavelength
Effective bandwidth
Slit width= Slit width= 3(
Pow
er
Slit width= (Slit width=
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Ab
sorb
ance
0.100
0.700
Wavelength, nm275220
(a)
0.5 nm bandwidth
Ab
sorb
ance
Wavelength, nm275220
0.100
0.600
(b)
1.0 nm bandwidth
Ab
sorb
ance
Wavelength, nm275220
0.100
0.600
(c)
2.0 nm bandwidth
Effect of Spectral Bandwidth
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Sample Containers
Cells or cuvettes that hold the samples must be made of materials that is transparent to radiation in the spectral
region of interest.
• Quartz or fused silica : UV region (below 350 nm) and also up to 3000 nm
•Silicate glasses: The region between 350 and 2000 nm•Plastic containers : Visible region
•Crystalline sodium Chloride : IR region
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Wavelength 100 180 380 850 2000 18000 40000
Region VUV UV Visible Near IR IR Far IR
Materials for cells, windows, lenses and prisms
LiF
NaCl
KBr
ZnSe
Fused silica or quartz
Corex glass
Silica glass
TlBr or TlI
Construction Materials for Spectroscopic Instruments
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Radiation Transducers
The detectors for early spectroscopic instruments were the
human eye or a photographic plate or film. These detection
devices have been largely supplanted by transducer that
convert radiant energy into an electrical signal. These
transducer are modern detectors.
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Res
pons
e
Power
1. High sensitivity
2. High signal to noise ratioNoise
Signal
Lower S/N
Higher S/N
3. Constant response over considerable range of wavelength
4. Fast response time
5. Zero output signal in the absence of illumination S = kP
S = kP+kd
Res
pons
e
Power6. The electrical signal would be directly proportional to radiant power
PowerR
espo
nse
Res
pons
e
WavelengthRes
pons
e
Time
Ideal Radiation Transducers
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Types of Radiation Transducers
• Photon transducers– UV, Vis, near IR
• Heat transducers– IR, far IR
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200 600 1000 1400 1800 2200 Wavelength nm
109
1013
1012
1011
1010
1014
1015
Photomultiplier tube
CdS photoconductivity cell
GaAs photovoltaic cell
silicon photodiode PbS photoconductivity cell
CdSe photoconductivity cell
Se/SeO photovoltaic cellThermocouple
Golay
Sp
ectr
al r
esp
onse
.
Response of Detectors
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Photon Transducers
• Photovoltaic cells
• Phototube
• Photomultiplier tubes
• Photoconductivity transducers
• Silicon photodiodes
• Charge-coupled device
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Plastic case
Glass Thin layer of silver
Selenium
Iron
+ -
Photovoltaic Cell
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Characteristics of Photovoltaic Cells
• Cell current = 10 – 100 mA
• No external electrical energy required.
• Usually used for low level signals.
• Low internal resistance = amplification not convenient
• Fatigue
• Low cost
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90 Vdc
Wire anodeCathode
Vacuum Photo Tube
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Sen
siti
vity
, mA
/w80
20
40
60
400 600 800 1000200
Wavelength, nm
K/Cs/Sb
Ga/As
Ag/O/Cs
Response of some Photoemissive Surfaces
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Dynode Potential(V) Number of electrons
1 90 1
2 180 10
3 270 100
4 360 103
5 450 104
6 540 105
7 630 106
8 720 107
9 810 108
Anode 900V Gain =108
12
3
4
5
6
8
7
9
Anode
PhotoemissiveCathode
GrillQuartz envelope
+_
900V dc
Anode
PhotoemissiveCathodeDynodes 1-9
To readout
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Features of Photomplier Tubes
• High sensitivity in UV, Vis, and NIR– Limited by dark current – Cooling to -30oC improves response
• Extremely fast time response
• Limited to measuring low-level signals
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p region n region
pn junction
Metal contactLead wire
Silicon Diode
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Forward bias
ee
Silicon Diode under Forward Bias
-+
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Depletion layer
Reverse bias
Silicon diode under Revese Bias
- +
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Photoconductivity Transducers
The most sensitive transducers for monitoring radiation in
the NIR region are semiconductors whose resistance
decrease when they absorb radiation within this range.
Absorption of radiation by these material promotes some
of their bound electrons into an energy state in which they
are free to conduct electricity. The resulting change in
conductivity can then be measured.
Examples:
CdS, CdSe, CdTe, PbS (specially sensitive at room temp.)
PbSe, InS, InSe.
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Thermal Transducers
This kind of transducers generally used in IR regions which photons
lack the energy to cause photoemission of the electrons.
The radiation impinges upon and is absorbed by a small black body,
and the resultant temperature is measured.
The heat capacity of the absorbing elements must be as small as
possible if a detectable temperature change is to be produced.
• Thermocouples
• Bolometers: resistance thermometer, Pt, Ni or semiconductor
(thermistor)
•Pyroelectric transducers
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+15V
-15V
To amplifier
Spectrophotometer slit
Thermocouple junction
Reference junction
+_
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Detectors
Photo- electric
Thermal
Photographic plates
Photomultiplier tube
Photo tubes
Photo cells
Photo diodes
Photo conductors
Golay pneumatic cell
Pyroelectric cell
Charge coupled devices
Wavelength 100 180 380 850 2000 18000 40000
Region VAC UV Visible Near IR IR Far IR
Detectors for Spectroscopic Instruments
Thermocouples or bolometers
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Signal Processors and Readouts
The signal processor is ordinarily an electronic device that amplifies the electronic signal from the transducer. In
addition, it may change the signal from dc to ac (or the reverse), change the signal phase, and filter it to remove
unwanted components.Also they may perform some mathematical operation on the signal such as differentiation or integration or conversion to
logarithm.
Readout devices are found in modern instrument. Some of them include digital meters, potentiometer or cathode array
tube.