Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr....
Transcript of Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr....
![Page 1: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/1.jpg)
2009 Cengage-Wadsworth
Chapter 6
Protein
![Page 2: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/2.jpg)
2009 Cengage-Wadsworth
Functional Categories
• Catalysts - enzymes– Hydrolases - cleave compounds– Isomerases - transfer atoms in a molecule– Ligases (synthases) - join compounds– Oxidoreductases - transfer electrons– Transferases - move functional groups
![Page 3: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/3.jpg)
2009 Cengage-Wadsworth
Functional Categories
• Messengers – Hormones
• Structural elements– Contractile proteins– Fibrous proteins– Globular proteins
• Immunoprotectors– Immunoproteins (antibodies)
![Page 4: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/4.jpg)
2009 Cengage-Wadsworth
Functional Categories
• Transporters– Albumin– Transthyretin (prealbumin)– Transferrin– Ceruloplasmin– Lipoproteins
![Page 5: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/5.jpg)
2009 Cengage-Wadsworth
Functional Categories
• Buffers– Regulation of acid-base balance
• Fluid balancers– Proteins attract water to blood
• Other roles– Adhesion, signaling, receptors, storage– Conjugated proteins
• Glycoproteins• Proteoglycans
![Page 6: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/6.jpg)
2009 Cengage-Wadsworth
Protein Structure & Organization
• Primary structure– Sequence of covalent bonds among amino acids
• Secondary structure– Hydrogen bonding– -helix– -conformation or -pleated sheet– Random coil
![Page 7: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/7.jpg)
Dr. Siham Gritly 7
Protein structure Each protein has a unique shape or conformation. all proteins
are composed exclusively of subunits of amino acids, which join together in long chains called polypeptides that fold or coil into
the unique shape of the functional protein
![Page 8: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/8.jpg)
Dr. Siham Gritly 8
1-Primary structure of proteinsamino acids sequences • The primary structure of a protein simply
consists of its linear sequence of amino acids; for example, "alanine-glycine-tryptophan-serine-glutamate-asparagine-glycine-lysine-…
![Page 9: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/9.jpg)
Dr. Siham Gritly 9
2-Secondary structure
• As peptide bonds are formed, aligning the amino acids, hydrogen bonds form between different amino acids in the chain.
• This bonding coils the polypeptide into the secondary structure of the protein, most commonly the alpha helix,
• The α-helix coils at every 4th amino acid.
![Page 10: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/10.jpg)
Dr. Siham Gritly 10
2-Secondary structure The α-helix coils of protein
![Page 11: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/11.jpg)
Dr. Siham Gritly 11
Pleated Proteinthe polypeptide have portions that lie parallel to each other (held by hydrogen bonds) instead of in the alpha helix, in which
the amino acids’ hydrogen bonds form a pleated structure. Fibrous proteins have significant pleated structures
![Page 12: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/12.jpg)
2009 Cengage-Wadsworth
Protein Structure & Organization• Tertiary structure
– Clustering of hydrophobic AAs toward center– Electrostatic (ionic) attraction– Strong covalent bonding between cysteine residues -
disulfide bridges• Quaternary structure
– Interactions between 2 or more polypeptide chains– Oligomers
![Page 13: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/13.jpg)
Dr. Siham Gritly 13
3-Tertiary Structure of protein• the side chains (the R groups) of amino acids may fold
independently into a functional unit called the domain.
• Domains are connected by the rest of the polypeptide. • The folding of a protein into its domains is related to
the hydrophilic or hydrophobic properties of its amino acids.
• Domain formation is part of the tertiary structure or proteins. globular shape (globulin)
![Page 14: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/14.jpg)
Dr. Siham Gritly 14
Tertiary Structure of protein
![Page 15: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/15.jpg)
Dr. Siham Gritly 15
4-Quaternary Protein Structure the structure formed by several protein molecules (polypeptide chains), usually called protein subunits
• If two or more polypeptide chains join in aggregate, they form a quaternary structure, such as in the protein molecule, hemoglobin.
• Often quaternary proteins are complexed with a different molecule, often a mineral. Hemoglobin contains iron, for example.
![Page 16: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/16.jpg)
Dr. Siham Gritly 16
4-Quaternary Protein Structure
Haemoglobin structure
![Page 17: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/17.jpg)
2009 Cengage-Wadsworth
Amino Acid Classification
• Structure– Central C– At least 1 amino group (NH2) – At least 1 carboxy (acid) group (COOH)– Side chain (R group)
• Makes AA unique
![Page 18: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/18.jpg)
Dr. Siham Gritly 18
Amino acid the building block
of proteins
![Page 19: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/19.jpg)
2009 Cengage-Wadsworth
Amino Acid Classification
• Net electrical charge– Zwitterions have none– Zwitterions: ion with a positive and a negative
charge• Polarity
– Polar or nonpolar– Determined by R group
• Essentiality– Lysine, threonine & histidine totally indispensable
![Page 20: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/20.jpg)
Dr. Siham Gritly 20
the carboxyl group is stronger acid than the amino group. At physiological pH (around 7.4) the carboxyl group will be unprotonated and the amino group will be protonated.
An amino acid with no ionizable R-group would be electrically neutral at this pH. This species is termed a zwitterion.
An amino acid in its (1) un-ionized and (2) zwitterionic forms
![Page 21: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/21.jpg)
Amino Acid classification based on polarity
27- 21
Non-Polar
Polar
UnchargedCysteineProlineSerine
GlutamineAsparagine
HydrophobicTryptophan
PhenylalanineIsoleucineTyrosineLeucineValine
Methionine
AmbivalentGlycine
ThreonineAlanine
ChargedArginine (+)
Glutamic acid (-)Aspartic Acid (-)
Lysine (+)Histidine (+)
![Page 22: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/22.jpg)
Essential AA Nonessential AAHistidine AlanineIsoleucine Arginine **Leucine AsparagineLysine Aspartic AcidMethionine Cysteine **Phenylalanine Glutamic acidThreonine Glutamine **Tryptophan Glycine **Valine Proline **
SerineTyrosine **
![Page 23: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/23.jpg)
2009 Cengage-Wadsworth
Sources of Protein
• Exogenous sources– Animal products - except fats– Plant products - grains/grain products, legumes,
vegetables• Endogenous proteins
– Desquamated mucoasal cells– Digestive enzymes & glycoproteins
![Page 24: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/24.jpg)
2009 Cengage-Wadsworth
Digestion & Absorption
• Protein digestion– Mouth & esophagus - none– Stomach
• HCl denatures• Pepsin hydrolyzes peptide bonds
![Page 25: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/25.jpg)
2009 Cengage-Wadsworth
Digestion & Absorption– Small intestine
• Pancreatic enzymes– Trypsinogen trypsin– Chymotrypsinogen chymotrypsin– Procarboxypeptidases A & B carboxypeptidases– Proelastase– Collagenase
• Brush border peptidases– Aminopeptidases, dipeptdylaminopeptidases, tripeptidases
• Tripeptides hydrolyzed or absorbed at brush border
![Page 26: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/26.jpg)
2009 Cengage-Wadsworth
Digestion & Absorption
• Intestinal brush border membrane amino acid & peptide absorption– Amino acid transport
• Carriers required - passive & active transporters– Peptide transport
• PEPT1 • Co-movement of protons (H+)
![Page 27: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/27.jpg)
2009 Cengage-Wadsworth
Digestion & Absorption
• Intestinal basolateral membrane transport of amino acids– Diffusion & sodium-independent transport are
main modes• Intestinal cell amino acid use
– Cells use or partially metabolize for release into blood
![Page 28: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/28.jpg)
2009 Cengage-Wadsworth
Digestion & Absorption
– Intestinal glutamine metabolism• Primary energy source for enterocytes
– Intestinal glutamate metabolism– Intestinal aspartame metabolism– Intestinal arginine metabolism– Intestinal methionine & cysteine metabolism
![Page 29: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/29.jpg)
2009 Cengage-Wadsworth
Digestion & Absorption
• Amino acid absorption into extraintestinal tissues– AAs enter portal vein to liver– Transport into hepatocytes– Transport into other cells– -glutamyl cycle
![Page 30: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/30.jpg)
2009 Cengage-Wadsworth
Amino Acid Metabolism
• Metabolism of AAs includes:– Protein synthesis– Amino acid catabolism– Hepatic catabolism
• Uses of aromatic amino acids• Uses of sulfur-containing amino acids• Uses of branched-chain amino acids• Uses of other amino acids
– Plasma amino acids & pools
![Page 31: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/31.jpg)
Nitrogen Pool
![Page 32: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/32.jpg)
2009 Cengage-Wadsworth
Synthesis of Plasma Proteins, Nitrogen-Containing Nonprotein Compounds, & Purine & Pyrimidine Bases
• Plasma proteins– Albumin– Transthyretin (prealbumin)– Retinol-binding protein– Blood clotting proteins– Immunoproteins– Transport proteins– Acute phase proteins– Stress (heat) shock proteins (hsp)
![Page 33: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/33.jpg)
2009 Cengage-Wadsworth
Synthesis of Plasma Proteins, Nitrogen-Containing Nonprotein Compounds, & Purine & Pyrimidine Bases
• Nitrogen-containing nonprotein compounds– Glutathione - antioxidant, reacts with H2O2, AA
transport, conversion of prostaglandin H2 to D2 & E2
– Carnitine - FA transport– Creatine - part of phosphocreatine (high-energy
compound)
![Page 34: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/34.jpg)
2009 Cengage-Wadsworth
Synthesis of Plasma Proteins, Nitrogen-Containing Nonprotein Compounds, & Purine & Pyrimidine Bases
– Carnosine - may be antioxidant– Choline - methyl donor, part of acetylcholine &
lecithin & sphingomyelin
![Page 35: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/35.jpg)
2009 Cengage-Wadsworth
Synthesis of Plasma Proteins, Nitrogen-Containing Nonprotein Compounds, & Purine & Pyrimidine Bases
• Purine & pyrimidine bases– Main constituents of DNA & RNA– Pyrimidines
• 6-membered rings containing N in positions 1 & 3• Uracil, cytosine & thymidine
– Purines• 2 fused rings, N in positions 1, 3, 7, 9• Adenine & guanine
![Page 36: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/36.jpg)
2009 Cengage-Wadsworth
Amino Acid Catabolism Overview
• Transamination &/or deamination of amino acids– Deamination = removal of amino group– Transamination = transfer of amino group from
one AA to AA carbon skeleton or -keto acid• Catalyzed by aminotransferases
![Page 37: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/37.jpg)
OVERVIEW OF AMINO ACID METABOLISM
ENVIRONMENT ORGANISM
Ingested protein
Bio- synthesis Protein
AMINO ACIDS
Nitrogen Carbonskeletons
Urea
Degradation (required)
1 2 3
a
b
PurinesPyrimidinesPorphyrins
c c
Used for energy
pyruvateα-ketoglutaratesuccinyl-CoAfumarateoxaloacetate
acetoacetateacetyl CoA
(glucogenic)(ketogenic)
![Page 38: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/38.jpg)
2009 Cengage-Wadsworth
Amino Acid Catabolism Overview• Disposal of ammonia--the urea cycle
– NH3 combines with CO2 or HCO3- to form carbamoyl
phosphate– Carbamoyl phosphate reacts with ornithine
transcarbamoylase (OTC) to form citruline– Aspartate reacts with citruline to form argininosuccinate– Arginosuccinate is cleaved to form fumarate & arginine– Urea is formed and ornithine is re-formed from cleavage of
arginine
![Page 39: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/39.jpg)
Steps of Urea Synthesis (cont...)
• Formation of urea & ornithine
![Page 40: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/40.jpg)
2009 Cengage-Wadsworth
Amino Acid Catabolism Overview• An overview of metabolism of the carbon
skeleton/-keto acid– Energy generation– Glucose & ketone body production– Cholesterol production– Fatty acid production
![Page 41: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/41.jpg)
FATE OF THE CARBON SKELETONS
Carbon skeletons are used for energy.
Glucogenic: TCA cycle intermediates
or pyruvate (gluconeogensis)
Ketogenic: acetyl CoA, acetoacetyl CoA,
or acetoacetate
![Page 42: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/42.jpg)
![Page 43: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/43.jpg)
Transamination
![Page 44: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/44.jpg)
2009 Cengage-Wadsworth
Hepatic Catabolism & Uses of Aromatic Amino Acids
• Phenylalanine & tyrosine– Phenylalanine converted to tyrosine by phenylalanine
hydroxylase– Tyrosine
• Degradation begins with transamination to p-hydroxyphenylpyruvate
• Tyrosine used in other tissues for synthesis of L-dopa & catecholamines
• Melanin, thyroid hormones– Disorders of phenylalanine & tyrosine metabolism
![Page 45: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/45.jpg)
2009 Cengage-Wadsworth
Hepatic Catabolism & Uses of Aromatic Amino Acids
• Tryptophan– Catabolized to N-formylkynurenine– This is catabolized to formate & kynurenine– Used for:
• Protein synthesis • Energy, glucose, & ketone body production• Synthesis of serotonin & melatonin
– Disorders of tryptophan metabolism.
![Page 46: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/46.jpg)
2009 Cengage-Wadsworth
Hepatic Catabolism & Uses of Sulfur (S)-Containing Amino Acids
• Methionine– Converted to S-adenosyl methionine
• SAM is principal methyl donor• Removal of methyl group yields S-adenosyl homocysteine (SAH)
– SAH converted to homocysteine– Homocysteine reacts with serine to form cystathionine– Cystathionine cleaved to form cysteine & -ketobutyrate– Propionyl CoA (made from -ketobutyrate) converted to D-
methylmalonyl CoA– Disorders of methionine metabolism
![Page 47: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/47.jpg)
2009 Cengage-Wadsworth
Hepatic Catabolism & Uses of Sulfur (S)-Containing Amino Acids
• Cysteine– Used for protein & glutathione synthesis– Converted to cysteine sulfinate, used to produce taurine– Taurine important in retina, functions as bile salt & inhibitory
neurotransmitter– Cysteine degradation yields pyruvate & sulfite
![Page 48: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/48.jpg)
2009 Cengage-Wadsworth
Hepatic Catabolism & Uses of the Branched-Chain Amino Acids
• Isoleucine, leucine, & valine• Taken up & transaminated primarily in
muscles
![Page 49: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/49.jpg)
2009 Cengage-Wadsworth
Hepatic Catabolism & Uses of Other Amino Acids
• Lysine– Ketogenic - catabolism yields acetyl CoA– Disorders of lysine metabolism
• Threonine– 3 pathways– Disorders of threonine metabolism
![Page 50: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/50.jpg)
2009 Cengage-Wadsworth
Hepatic Catabolism & Uses of Other Amino Acids
• Glycine & serine– Produced from one another in reversible reaction
requiring folate– Disorders of glycine metabolism
• Arginine– Kidney - creatine synthesis– Liver - generation of urea & ornithine
• Histidine– Glutamate, carnosine, histamine
![Page 51: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/51.jpg)
2009 Cengage-Wadsworth
Amino Acids Not Taken Up by the Liver: Plasma Amino Acids & Amino Acid Pool(s)
• Plasma concentrations rise after a meal• Pool of about 150 g of endogenous +
exogenous AAs • Re-use thought to be primary source of AAs
for protein synthesis• More nonessential than essential in pool
![Page 52: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/52.jpg)
2009 Cengage-Wadsworth
Interorgan “Flow” of Amino Acids & Organ-Specific Metabolism
• Glutamine & the liver, kidneys, & intestine– Ammonia transport– Hypercatabolic conditions
• Alanine & the liver & muscle– Inter-tissue transfer of amino groups– Liver: converted to glutamate or glucose
![Page 53: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/53.jpg)
2009 Cengage-Wadsworth
Interorgan “Flow” of Amino Acids & Organ-Specific Metabolism
• Skeletal muscle– Isoleucine, leucine, & valine– Nitrogen-containing compounds as indicators of
muscle mass & muscle/ protein catabolism
![Page 54: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/54.jpg)
2009 Cengage-Wadsworth
Interorgan “Flow” of Amino Acids & Organ-Specific Metabolism
• Kidneys– Serine synthesis from glycine– Glycine catabolism to ammonia– Histidine generation from carnosine degradation– Arginine synthesis from citruline– Tyrosine synthesis from phenylalanine– Guanidoacetate formation from arginine & glycine for
creatine synthesis
![Page 55: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/55.jpg)
2009 Cengage-Wadsworth
Brain & Accessory Tissues• Biogenic amines & neurotransmitters/hormones
– Tryptophan - melatonin & serotonin– Tyrosine - dopamine, norepinephrine, epinephrine– Glysine - inhibitory neurotransmitter– Taurine - inhibitory neurotransmitter– Aspartate - excitatory neurotransmitter– Glutamate - excitatory neurotransmitter or converted to -
amino butyric acid (GABA)
![Page 56: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/56.jpg)
2009 Cengage-Wadsworth
Brain & Accessory Tissues
• Neuropeptides– Hormone-releasing factors– Endocrine effects– Modulatory actions on transmitter functions,
mood or behavior– Neurosecretory cells of hypothalamus secrete– Synthesized from AAs via DNA codes
![Page 57: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/57.jpg)
2009 Cengage-Wadsworth
Protein Turnover: Synthesis & Catabolism of Tissue Proteins
• Food intake & nutritional status• Hormonal mediation• AA pools connect 2 cycles of N metabolism:
– Protein turnover– Nitrogen balance
• Protein synthesis & degradation controlled separately
![Page 58: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/58.jpg)
2009 Cengage-Wadsworth
Protein Turnover: Synthesis & Catabolism of Tissue Proteins
• Cellular protein degradation systems– Lysosomal degradation– Proteasomal degradation– Calcium or calcium-activated proteolytic
degradation
![Page 59: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/59.jpg)
2009 Cengage-Wadsworth
Changes in Body Mass with Age• Lean body mass increases throughout childhood
– Changes in total fluid & ECF/ICF• Gender differences develop during adolescence
– Greater increase in males• After 25, weight gain = fat gain• Lean mass decreases with increasing age
– More so in women than men– Body water declines too
![Page 60: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/60.jpg)
2009 Cengage-Wadsworth
Protein Quality & Protein Intake
• Foods can be categorized as:– High-quality or complete proteins– Low-quality or incomplete proteins
• Evaluation of protein quality– Nitrogen balance/nitrogen status– Chemical or amino acid score– Protein digestibility corrected amino acid score
![Page 61: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/61.jpg)
2009 Cengage-Wadsworth
Protein Quality & Protein Intake
– Protein efficiency ratio– Biological value– Net protein utilization– Net dietary protein calories percentage
• Protein information on food labels– % Daily Value
![Page 62: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/62.jpg)
2009 Cengage-Wadsworth
Protein Quality & Protein Intake
• Recommended protein & amino acid intakes– RDA for adults = 0.8 g/kg– AI for birth-6 months– RDA for indispensible AAs – Negative effects of high protein intakes
controversial (no UL)– AMDR = 10%-35% kcal
![Page 63: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/63.jpg)
2009 Cengage-Wadsworth
Protein Quality & Protein Intake
• Protein deficiency/malnutrition– Kwashiorkor
• Adequate energy with insufficient protein• Edema owing to loss of blood proteins
– Marasmus• Wasting, emaciation• Chronic insufficiency of energy & protein
![Page 64: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/64.jpg)
2009 Cengage-Wadsworth
Perspective 6
Protein Turnover:Starvation Compared with Stress
![Page 65: Chapter 6 - kaunsalamoudi.kau.edu.sa/GetFile.aspx?id=196004&Lng=AR… · PPT file · Web viewDr. Siham Gritly 1-Primary structure of proteinsamino acids sequences The primary structure](https://reader034.fdocuments.us/reader034/viewer/2022051601/5ada0a867f8b9a137f8ce744/html5/thumbnails/65.jpg)
2009 Cengage-Wadsworth
Starvation vs. Stress• Starvation
– Protein synthesis decreases– Hormone balance adjusts– Adaptation - muscle catabolism slows
• Stress– Hypermetabolism– Lipolysis doesn’t lead to ketosis– Muscle catabolism undiminished– Protein turnover - immune response & acute phase
response