CHAPTER 5 The Working Cell Overview: Energy Def Laws Chemical Reactions ATP Enzymes Def Activity...
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Transcript of CHAPTER 5 The Working Cell Overview: Energy Def Laws Chemical Reactions ATP Enzymes Def Activity...
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CHAPTER 5The Working Cell
Overview: Energy
DefLawsChemical ReactionsATP
EnzymesDefActivity
MembraneStructureFunctionTransport (passive, active, Osmosis, exo- and endocytosis)
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– What is energy?
• Energy is defined as the capacity to do work
• All organisms require energy to stay alive
• Energy makes change possible
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• Kinetic energy is the energy of motion
• Potential energy is stored energy
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• First law of thermodynamics:
Energy can be changed from one form to another– However, energy cannot be created or destroyed
Two laws govern energy conversion
• Second law of thermodynamics:
Energy changes are not 100% efficient– Energy conversions increase disorder, or entropy – Some energy is always lost as heat
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• There are two types of chemical reactions:
– Endergonic reactions absorb energy and yield products rich in potential energy
– Exergonic reactions release energy and yield products that contain less potential energy than their reactants
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• Cellular respiration
– Is the energy-releasing chemical breakdown of fuel molecules
– Provides energy for the cell to do work
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• Cells carry out thousands of chemical reactions – The sum of these reactions constitutes cellular
metabolism
Chemical reactions either store or release energy
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• In cellular respiration, some energy is stored in ATP molecules
• ATP powers nearly all forms of cellular work
• ATP molecules are the key to energy coupling
ATP shuttles chemical energy within the cell
• The chemical energy of organic molecules is released in cellular respiration to make ATP in the mitochondria
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• ATP (adenosine triphosphate)
The Structure of ATP
– Consists of adenosine plus a tail of three phosphate groups
– Is broken down to ADP, accompanied by the release of energy
• When the bond joining a phosphate group to the rest of an ATP molecule is broken by hydrolysis, the reaction supplies energy for cellular work
• ATP can energize other molecules by transferring phosphate groups
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• For a chemical reaction to begin, reactants must absorb some energy– This energy is called the energy of activation (EA) – This represents the energy barrier that prevents
molecules from breaking down spontaneously
Enzymes speed up the cell’s chemical reactions by lowering energy barriers
ENZYMES
• Activation energy
– Is the energy that activates the reactants– Triggers a chemical reaction
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• Enzymes– Lower the activation energy for chemical reactions
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• Enzymes are selective– This selectivity determines which chemical reactions
occur in a cell
A specific enzyme catalyzes each cellular reaction
• Each enzyme recognizes a specific substrate
– The active site fits to the substrate, and the enzyme changes shape slightly
– This interaction is called induced fit
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• Enzyme activity is influenced by – temperature – concentration – pH
• Some enzymes require nonprotein cofactors– Some cofactors are organic molecules called
coenzymes
The cellular environment affects enzyme activity
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• Enzyme inhibitors
Enzyme Inhibitors
– Can inhibit a metabolic reaction– Bind to the active site, as substrate impostors
• Other inhibitors
– Bind at a remote site, changing the enzyme’s shape– In some cases, this is called feedback regulation
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• Membranes organize the chemical reactions making up metabolism
Membranes organize the chemical activities of cells
MEMBRANE STRUCTURE AND FUNCTION
Cytoplasm
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• Membranes are selectively permeable
– They control the flow of substances into and out of a cell
• Membranes can hold teams of enzymes that function in metabolism
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• Phospholipids are the main structural components of membranes
• They each have a hydrophilic head and two hydrophobic tails
Membrane phospholipids form a bilayer
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• In water, phospholipids form a stable bilayer
– The heads face outward and the tails face inward
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• Phospholipid molecules form a flexible bilayer– Cholesterol and protein molecules are embedded in it – Carbohydrates act as cell identification tags
The membrane is a fluid mosaic of phospholipids and proteins
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• Some membrane proteins form cell junctions
• Others transport substances across the membrane
Proteins make the membrane a mosaic of function
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• Many membrane proteins are enzymes
• Some proteins function as receptors for chemical messages from other cells
– The binding of a messenger to a receptor may trigger signal transduction
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• In passive transport, substances diffuse through membranes without work by the cell– They spread from
areas of high concentration to areas of lower concentration
Passive transport is diffusion across a membrane
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• Diffusion is one result of the movement of molecules
– Molecules tend to spread into the available space– Diffusion is passive transport; no energy is needed
• Another type of passive transport is facilitated diffusion, the transport of some substances by specific transport proteins that act as selective corridors
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• In osmosis, water travels from an area of lower solute concentration to an area of higher solute concentration
Osmosis is the passive transport of water
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• Osmosis causes cells to shrink in a hypertonic (has a higher concentration of solute) solution and swell in a hypotonic (has a lower concentration of solute) solution The control of water balance (osmoregulation) is essential for organisms
Water balance between cells and their surroundings is crucial to organisms
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• Small nonpolar molecules diffuse freely through the phospholipid bilayer
• Many other kinds of molecules pass through selective protein pores by facilitated diffusion
Transport proteins facilitate diffusion across membranes
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• Active transport requires energy to move molecules across a membrane against a concentration gradient
Active Transport: the Pumping of Molecules Across Membranes
• Active transport in two solutes across a membrane
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• To move large molecules or particles through a membrane– a vesicle may fuse with the membrane and expel its
contents (exocytosis: Secretes substances outside of the cell)
Exocytosis and endocytosis transport large molecules
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– or the membrane may fold inward, trapping material from the outside (endocytosis: takes material into the cell)
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• Three kinds of endocytosis
• Receptor-mediated endocytosis
– Is triggered by the binding of external molecules to membrane proteins
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• In phagocytosis (“cellular eating”) a cell engulfs a particle and packages it within a food vacuole
• In pinocytosis (“cellular drinking”) a cell “gulps” droplets of fluid by forming tiny vesicles
Food being ingested
Pseudopodof amoeba
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• Enzymes and membranes are central to the processes that make energy available to the cell
• Chloroplasts carry out photosynthesis, using solar energy to produce glucose and oxygen from carbon dioxide and water
• Mitochondria consume oxygen in cellular respiration, using the energy stored in glucose to make ATP
Chloroplasts and mitochondria make energy available for
cellular work
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• Nearly all the chemical energy that organisms use comes ultimately from sunlight
• Chemicals recycle among living organisms and their environment