Chang Sha, China

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MICHAEL COHRS APPLICATIONS ENGINEER – AQUATICS MARKETS FLUID IMAGING TECHNOLOGIES DYNAMIC IMAGING PARTICLE ANALYSER FOR ALGAL RESEARCH, MONITORING AND ANALYSIS

Transcript of Chang Sha, China

MICHAEL COHRSAPPLICATIONS ENGINEER – AQUATICS MARKETS

FLUID IMAGING TECHNOLOGIES

DYNAMIC IMAGING PARTICLE ANALYSER FOR ALGAL RESEARCH,

MONITORING AND ANALYSIS

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F lu id Imag ing Techno log ies Fo u n d e d i n 1 9 9 9 M a i n e , U S A ( B i g e l o w

L a b o r a t o r y ) ( F l o w C y t o m e t e r A n d

M i c r o s c o p e ( F l o w C a m ® ) 5 0 0 + F l o w C a m s S o l d A p p l i e d I n d u s t r i e s

A q u a t i c I n d u s t r i a l s O i l a n d G a s P r o c e s s Wa t e r

FLOWCAM® IMAGING SYSTEM

FlowCAM®

FlowCam SV “High Sensitivity FlowCam” (HSFC)

Sample

Dynamic Imaging Particle Analysis

(DIPA) Constant Fluidic Motion

Particle Image Isolation

Image Acquisition and Analysis

40+ Size and Shape Measurements

Quantification and Classification Software

Computer

THE FLOWCAM® IMAGING SYSTEMTechnical Specs

• Fully self-contained (computer, monitor, etc. included)

• Four magnifications (2X, 4X, 10X and 20X) available to accommodate broad range of sample particle sizes (2µm to 2mm)

• Automatic Classification from Preset Filters

• Intuitive VisualSpreadsheet® software for data capture and analysis

• Many options available including cross-polarized illumination, 488 or 532nm laser, variable emission filters, color camera and many different flow cell sizes

• Processing Capability –• Flow – .1 ml/min to 12 ml/min• Concentration – 5,000,000 particles/ml

Quick Image Acquisition

http://www.youtube.com/watch?v=STp2r5aotfk&feature=youtu.be

FLUID IMAGING TECHNOLOGIES WATER TREATMENT GRANT PROGRAM

Grant Recipient

NJ American WaterTreatment Facility in Northern New Jersey

Supervising Agent

Scott BrezinskiWater Quality SupervisorPassaic Operating Area

Project Intern

Michael CohrsPhysics Graduate StudentMontclair State University

Evaluate an Optimal Equipment Setup Develop and Publish an Effective SOP Collect, Analyze, and Interpret Effective Data Publish and Present Results

Algae at the Root of Everyday Issues

Taste and OdorShort Filter Runs Manganese Spikes

Can Careful Monitoring of Algal Population Dynamics Lead To Better Environmental Management Solutions?

June   July   August   September   October  1         AP 9     AP 242         AP 24     AP 243     NAPS 20     AP 24 AP - 10x 244             AP 24 AP 125         AP 24 AP 24    6         AP 24 AP 24    7         AP 24        8     NAPS 20 AP 24        9         AP 24 AP 24    

10     NAPS 20     AP 24 Noon - 10x 6 * Van Dorn Start 7/17 & PPML excel11             AP 24     ** Start of Crustecea 7/3012     NAPS 20 AP 24 AP 24     *** FC 200 Start 8/2113         AP 24 ****AP 24     ****Manganese Start14         AP 24        15     NAPS 20 AP 24         NAPS = NOON/AM/PM/Surface16         AP 24 AP 24     AP = AM/PM17     * AP 24     AP 24    18             AP 24    19         AP 24 AP 24    20         AP 24 AP 24    21         *** AP 24        22         AP 24        23     Bill   AP 24 AP 24    24 N-A-P-S 3 AP-10x 24     AP 24    25     AP 24     AP 24    26 NAPS 20 AP 24 AP 24        27         AP 24        28 NAPS 20     AP 24        29     AP 24 AP 24        

30    **AP-10x-

4x/100 24     AP 24    31     AP 24             Total

Total Files 43 268 489 432 90 1322Total Days 3 13 21 18 5

Sample Schedule 2013

Traditional Light Microscopy

Time Consuming Procedure Vague Approximations Extreme Human Factor Unassisted Morphology One and Done

FlowCAM® Imaging System

3 Samples Run in Under 10 Min. Internal Cytometer Limiting Human Error 40+ Digital Parameters Assist Morphology Digital Memory Last a Long Time

Elevating An Old Technology

ALGAE CLASSIFICATION AND QUANTIFICATION

Auto-Classification

Classification Window for Quick and Automatic Classification Export Count Summaries to Excel Worksheet Build Filters for Specific Parameters of Interest

Build Your Own Image Libraries

Isolate Selected Images from Multiple Samples Into One Easy to Access Database Use Libraries to Create Filters for Simple Classification

Easy Sort and Data Recording

Sorting Using Preset Filter Unique to Your Images Sort and Filter Through over 40 Digital Parameters Easy Sorting by Statistical Algorithms

CYANOBACTERIA MONITORING

Accurate Population Dynamics So You Know When to Test and Treat

Save Money on Cyanotoxin Tests Treat Only When Necessary Predict Taste and Odor Issues

Microcystis Aphanizomenon Anabaena

6/28/1

3C7/8

/13C

7/26/1

3C8/5

/13C

8/22/1

3C9/6

/13C

9/25/1

3C

10/3/1

3C0

500

1000

1500

2000

2500

AnabaenaAphanizomenonMicrocystis

August 23rd, 2013

4Ox - Magnification

August 26th, 2013

4Ox - Magnification

August 28th, 2013

4Ox - Magnification

100x - Magnification

100x - Magnification

6/28/13C 7/8/13C 7/26/13C 8/5/13C 8/22/13C 9/6/13C 9/25/13C 10/3/13C0

500

1000

1500

2000

2500

AnabaenaAphanizomenonMicrocystis

Preventative Measures

Reactive Measures

Num

ber o

f Par

ticle

s

Can Careful Monitoring of Algal Population Dynamics Lead To Better Environmental Management Solutions?

• USE OF COPPER SULFATE AND CUTRINE ONLY PARTIALLY EFFECTIVE

• BUDGET ~$60,000/YR FOR THESE CHEMICALS

• ENVIRONMENTAL IMPACTS

• ADDITION OF CHEMICAL CAUSES RELEASE OF TASTE AND ODOR METABOLITES (AND POSSIBLY TOXINS) INTO WATER COLUMN

• CAN LEAD TO DEVELOPMENT OF COPPER-RESISTANT ORGANISMS

Reactive MeasuresKill Bloom with Chemical Treatment

Diatoms GoldenGreen Algae

Cyanobacteria Others Total

5/9/14 391 5 0 0 18 4145/14/14 95 53 0 0 85 2335/16/14 50 74 0 0 70 1955/21/14 18 279 0 1 38 3365/28/14 5 243 0 43 7 2986/4/14 165 89 4 38 29 326

6/11/14 16 0 6 7 195 2256/18/14 2 1 12 6 398 4196/25/14 213 1 53 219 468 9547/2/14 88 0 302 802 578 1,7707/9/14 52 0 76 680 634 1,442

7/16/14 5 0 35 298 562 9007/23/14 1 0 8 264 149 4227/30/14 5 0 10 554 153 7228/6/14 0 0 1 750 52 802

8/13/14 1 0 12 1,069 1,323 2,4058/21/14 1 0 2 3,043 184 3,2308/27/14 5 0 2 8,179 276 8,4639/3/14 2 0 12 23,259 426 23,699

9/10/14 3 0 8 23,761 2,356 26,1289/17/14 0 0 1 43,602 1,291 44,8949/24/14 0 0 3 2,934 662 3,59810/1/14 0 0 0 2,348 737 3,085

6/28/1

3C7/8

/13C

7/26/1

3C8/5

/13C

8/22/1

3C9/6

/13C

9/25/1

3C

10/3/1

3C0

500

1000

1500

2000

2500

Preventative:Sound Wave Frequency Algal Control

Water MonitoringFlowCam®

Applications Aquatic Research for Aquatic Microorganisms

Discreet or Continuous Sampling – In Lab, At Sea

HAB Monitoring Classification and Quantification

Algae Cultures and Algal Research Algae-to-BioFuels, Nutraceuticals, etc.

Water Treatment (Drinking) Taste and Odor Algae and

Cyanobacteria Monitoring; Particle Removal Analysis

Paleolimnology

Waste Water Analysis Monitor Microbial Activity and

Total Suspended Solids Methane Production

Ballast Water Testing and Research Viability Analysis – FDA,

Neutral Red, etc.

Microplastics Presence and Identification

Training and Education

Zooplankton Analysis

Anabaena, Newnan’s Lake, Florida

Karenia BrevisGulf of Mexico, November 11,

2011

Harmful Algal Bloom Monitoring

Algae Technology

Contaminant Monitoring

Growth Rate Monitoring

Lipid Analysis

Invasive SpeciesMonitoring:Veliger Mussels

MicroplasticsPresence and Identification

Aulacoseira and Pollen Fossils

PaleoLimnology

THANK YOUw w w. fl u i d i m a g i n g . c o m