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Transcript of Chang Sha, China
MICHAEL COHRSAPPLICATIONS ENGINEER – AQUATICS MARKETS
FLUID IMAGING TECHNOLOGIES
DYNAMIC IMAGING PARTICLE ANALYSER FOR ALGAL RESEARCH,
MONITORING AND ANALYSIS
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F lu id Imag ing Techno log ies Fo u n d e d i n 1 9 9 9 M a i n e , U S A ( B i g e l o w
L a b o r a t o r y ) ( F l o w C y t o m e t e r A n d
M i c r o s c o p e ( F l o w C a m ® ) 5 0 0 + F l o w C a m s S o l d A p p l i e d I n d u s t r i e s
A q u a t i c I n d u s t r i a l s O i l a n d G a s P r o c e s s Wa t e r
Sample
Dynamic Imaging Particle Analysis
(DIPA) Constant Fluidic Motion
Particle Image Isolation
Image Acquisition and Analysis
40+ Size and Shape Measurements
Quantification and Classification Software
Computer
THE FLOWCAM® IMAGING SYSTEMTechnical Specs
• Fully self-contained (computer, monitor, etc. included)
• Four magnifications (2X, 4X, 10X and 20X) available to accommodate broad range of sample particle sizes (2µm to 2mm)
• Automatic Classification from Preset Filters
• Intuitive VisualSpreadsheet® software for data capture and analysis
• Many options available including cross-polarized illumination, 488 or 532nm laser, variable emission filters, color camera and many different flow cell sizes
• Processing Capability –• Flow – .1 ml/min to 12 ml/min• Concentration – 5,000,000 particles/ml
Quick Image Acquisition
http://www.youtube.com/watch?v=STp2r5aotfk&feature=youtu.be
FLUID IMAGING TECHNOLOGIES WATER TREATMENT GRANT PROGRAM
Grant Recipient
NJ American WaterTreatment Facility in Northern New Jersey
Supervising Agent
Scott BrezinskiWater Quality SupervisorPassaic Operating Area
Project Intern
Michael CohrsPhysics Graduate StudentMontclair State University
Evaluate an Optimal Equipment Setup Develop and Publish an Effective SOP Collect, Analyze, and Interpret Effective Data Publish and Present Results
Algae at the Root of Everyday Issues
Taste and OdorShort Filter Runs Manganese Spikes
Can Careful Monitoring of Algal Population Dynamics Lead To Better Environmental Management Solutions?
June July August September October 1 AP 9 AP 242 AP 24 AP 243 NAPS 20 AP 24 AP - 10x 244 AP 24 AP 125 AP 24 AP 24 6 AP 24 AP 24 7 AP 24 8 NAPS 20 AP 24 9 AP 24 AP 24
10 NAPS 20 AP 24 Noon - 10x 6 * Van Dorn Start 7/17 & PPML excel11 AP 24 ** Start of Crustecea 7/3012 NAPS 20 AP 24 AP 24 *** FC 200 Start 8/2113 AP 24 ****AP 24 ****Manganese Start14 AP 24 15 NAPS 20 AP 24 NAPS = NOON/AM/PM/Surface16 AP 24 AP 24 AP = AM/PM17 * AP 24 AP 24 18 AP 24 19 AP 24 AP 24 20 AP 24 AP 24 21 *** AP 24 22 AP 24 23 Bill AP 24 AP 24 24 N-A-P-S 3 AP-10x 24 AP 24 25 AP 24 AP 24 26 NAPS 20 AP 24 AP 24 27 AP 24 28 NAPS 20 AP 24 29 AP 24 AP 24
30 **AP-10x-
4x/100 24 AP 24 31 AP 24 Total
Total Files 43 268 489 432 90 1322Total Days 3 13 21 18 5
Sample Schedule 2013
Traditional Light Microscopy
Time Consuming Procedure Vague Approximations Extreme Human Factor Unassisted Morphology One and Done
FlowCAM® Imaging System
3 Samples Run in Under 10 Min. Internal Cytometer Limiting Human Error 40+ Digital Parameters Assist Morphology Digital Memory Last a Long Time
Elevating An Old Technology
ALGAE CLASSIFICATION AND QUANTIFICATION
Auto-Classification
Classification Window for Quick and Automatic Classification Export Count Summaries to Excel Worksheet Build Filters for Specific Parameters of Interest
Build Your Own Image Libraries
Isolate Selected Images from Multiple Samples Into One Easy to Access Database Use Libraries to Create Filters for Simple Classification
Easy Sort and Data Recording
Sorting Using Preset Filter Unique to Your Images Sort and Filter Through over 40 Digital Parameters Easy Sorting by Statistical Algorithms
CYANOBACTERIA MONITORING
Accurate Population Dynamics So You Know When to Test and Treat
Save Money on Cyanotoxin Tests Treat Only When Necessary Predict Taste and Odor Issues
Microcystis Aphanizomenon Anabaena
6/28/1
3C7/8
/13C
7/26/1
3C8/5
/13C
8/22/1
3C9/6
/13C
9/25/1
3C
10/3/1
3C0
500
1000
1500
2000
2500
AnabaenaAphanizomenonMicrocystis
6/28/13C 7/8/13C 7/26/13C 8/5/13C 8/22/13C 9/6/13C 9/25/13C 10/3/13C0
500
1000
1500
2000
2500
AnabaenaAphanizomenonMicrocystis
Preventative Measures
Reactive Measures
Num
ber o
f Par
ticle
s
Can Careful Monitoring of Algal Population Dynamics Lead To Better Environmental Management Solutions?
• USE OF COPPER SULFATE AND CUTRINE ONLY PARTIALLY EFFECTIVE
• BUDGET ~$60,000/YR FOR THESE CHEMICALS
• ENVIRONMENTAL IMPACTS
• ADDITION OF CHEMICAL CAUSES RELEASE OF TASTE AND ODOR METABOLITES (AND POSSIBLY TOXINS) INTO WATER COLUMN
• CAN LEAD TO DEVELOPMENT OF COPPER-RESISTANT ORGANISMS
Reactive MeasuresKill Bloom with Chemical Treatment
Diatoms GoldenGreen Algae
Cyanobacteria Others Total
5/9/14 391 5 0 0 18 4145/14/14 95 53 0 0 85 2335/16/14 50 74 0 0 70 1955/21/14 18 279 0 1 38 3365/28/14 5 243 0 43 7 2986/4/14 165 89 4 38 29 326
6/11/14 16 0 6 7 195 2256/18/14 2 1 12 6 398 4196/25/14 213 1 53 219 468 9547/2/14 88 0 302 802 578 1,7707/9/14 52 0 76 680 634 1,442
7/16/14 5 0 35 298 562 9007/23/14 1 0 8 264 149 4227/30/14 5 0 10 554 153 7228/6/14 0 0 1 750 52 802
8/13/14 1 0 12 1,069 1,323 2,4058/21/14 1 0 2 3,043 184 3,2308/27/14 5 0 2 8,179 276 8,4639/3/14 2 0 12 23,259 426 23,699
9/10/14 3 0 8 23,761 2,356 26,1289/17/14 0 0 1 43,602 1,291 44,8949/24/14 0 0 3 2,934 662 3,59810/1/14 0 0 0 2,348 737 3,085
6/28/1
3C7/8
/13C
7/26/1
3C8/5
/13C
8/22/1
3C9/6
/13C
9/25/1
3C
10/3/1
3C0
500
1000
1500
2000
2500
Preventative:Sound Wave Frequency Algal Control
Water MonitoringFlowCam®
Applications Aquatic Research for Aquatic Microorganisms
Discreet or Continuous Sampling – In Lab, At Sea
HAB Monitoring Classification and Quantification
Algae Cultures and Algal Research Algae-to-BioFuels, Nutraceuticals, etc.
Water Treatment (Drinking) Taste and Odor Algae and
Cyanobacteria Monitoring; Particle Removal Analysis
Paleolimnology
Waste Water Analysis Monitor Microbial Activity and
Total Suspended Solids Methane Production
Ballast Water Testing and Research Viability Analysis – FDA,
Neutral Red, etc.
Microplastics Presence and Identification
Training and Education
Anabaena, Newnan’s Lake, Florida
Karenia BrevisGulf of Mexico, November 11,
2011
Harmful Algal Bloom Monitoring
Predicting Methane
Production
Bacilli-Shaped Microorganisms