Performance Report for Cooperative Agreement No: NA06OAR4810163 for the Period from March 1, 2007 to August 31, 2007

University of Maryland Eastern Shore

Living Marine Resources Cooperative Science Center

Paulinus Chigbu, Ph.D. (Principal Investigator & Director, LMRCSC)

Eric B. May (Co-Principal Investigator & Distinguished Research Scientist) University of Maryland Eastern Shore (Lead Institution)

Dewayne Fox, Ph.D. (Co-Principal Investigator)

Delaware State University

George Burbanck, Ph.D. (Co-Principal Investigator) Hampton University

Dionne Hoskins, Ph.D. (Co-Principal Investigator)

Savannah State University

Rosemary Jagus, Ph.D. (Co-Principal Investigator) Center of Marine Biotechnology, UMBI

David Die, Ph.D. (Co-Principal Investigator)

RSMAS/University of Miami

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Table of Contents

List of Tables 2 Executive Summary 3 Introduction 5 Goals and Objectives 5 Section I: Status of Award Activities 5 Goal 1: Enhance Marine Research and Academic Program Capacities of the Center MSIs 6

Objective 1: Building on Existing Academic and Organizational Infrastructure to Develop Strong Interactive Academic Programs in Marine Sciences 6

• Increased use of Virtual Campus 6 • Academic Courses 7 • Strengthening Administrative and Programmatic Components of the Center 8 A: Administrative 8 B: Programmatic 9

Goal 2: Enhance Research Programs in the Marine Sciences at Participating Institutions Objective 1: Continue Current and Develop New Research Programs Congruent with NOAA Fisheries Research Priorities 12

• TAB approved projects-Year 1 Status 15 • Scholarly Productivity 32 • Presentations 32 • Publications 34 • Grantsmanship 36

Objective 2: Fully Integrate Research Programs with NOAA Fisheries to Ensure Long Term Funding and Programmatic Stability 40

Section II: Education and Outreach Efforts 40 Goal 3: Generate a Pool of Scholars Entering the Field of Marine Sciences and Fisheries 40 Objective 1: Enhance Recruitment of Students from Underrepresented Groups into marine science disciplines 40

Objective 2: Strengthen Outreach & Education Programs to students and the public 44 Objective 3: Develop Programs that Enhance the Practical Education of Undergraduate and Graduate Students in the Marine Sciences 47

Section III: Success Stories 48 Section IV. Amendments to Award 50 Appendices 51

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List of Tables Table 1. LMRCSC Spring 2007 Seminar Series 6 Table 2. Harmful Algal Bloom seminar series at COMB 7 Table 3. Courses Offered in Spring Semester 2007 Using Interactive Video Network (IVN) 7 Table 4. Courses Offered in fall Semester 2007 Using Interactive Video Network (IVN) 7 Table 5: LMRCSC projects approved by Technical Advisory Board for 2006/2007 14 Table 6: Current leveraged funding to LMRCSC institutions 36 Table 7: Grants submitted during the current reporting period 38 Table 8: Students recruited into the LMRCSC program during the current reporting period. 41 Table 9: Students who graduated during this reporting period 42 Table 10: Students who worked in labs of LMRCSC scientists who served as their mentors And have received stipend support during this performance period 42 Table 11: Students who participated in internships at NOAA or other agencies 44 Table 12: Community Service Activities 46 Table 13: Students who participated in research cruises aboard NOAA vessels 48

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Executive Summary The mission of the Living Marine Resources Cooperative Science Center (LMRCSC) is to conduct research congruent with the interests of NOAA Fisheries and to prepare students for careers in research, management, and public policy that support the sustainable harvest and conservation of our nation's living marine resources. Established in October, 2001, the LMRCSC was created as a cooperative agreement between NOAA Educational Partnership Program (NOAA EPP), the University of Maryland Eastern Shore (UMES), Delaware State University (DSU), Hampton University (HU), Savannah State University (SSU), the University of Miami (UM/RSMAS) and the University of Maryland Biotechnology Institute Center of Marine Biotechnology (COMB). UMES is the lead institution. The mission of the LMRCSC is accomplished by addressing the following goals: Goal 1: Enhance Marine Research and Academic Program Capacities of the Center MSIs

Goal 2: Enhance Research Programs in the Marine Sciences at Participating Institutions

Goal 3: Generate a Pool of Scholars Entering the Field of Marine Sciences and Fisheries The Center continues to be guided by two management tracks, A) Administrative and B) Programmatic. The Administrative component includes the Center Director, Program Manager, Executive Committee, Center Core Administration and the Board of Visitors.

To accomplish Goal 1, the Center increased use of the Virtual Campus for curriculum development and meetings. Seminars and several courses offered through the University of Maryland interactive video network (IVN) were made available to Center students during this reporting period.

In support of the programmatic component of the LMRCSC designed to accomplish Goal 2, the Technical Advisory Board (TAB) reviews and provides recommendations on the Center’s science plan and research proposals. Cooperative workshops are conducted annually or biannually to address four research thematic areas of the LMRCSC: Quantitative Fisheries, Essential Fish Habitat, Aquaculture and Fisheries Socioeconomics.

The on-going and future research projects at the Center are intended to meet the mission of NOAA Fisheries: “Stewardship of living marine resources through science-based conservation and management and the promotion of healthy ecosystems”. The TAB and NOAA scientists’ involvement also ensures that the LMRCSC has a strong linkage with the mission of NOAA: “To understand and predict changes in Earth’s environment and conserve and manage coastal and marine resources to meet our Nation’s economic, social and environmental needs”. During this reporting period, no cooperative workshops were organized. However, the LMRCSC Request for Proposals was rewritten with specific guidance designed to foster collaboration amongst Center institutions and with NOAA. This announcement was distributed to Center and selected NOAA scientists on August 8, 2007.

In the current reporting period, LMRCSC students and faculty made 37 presentations at scientific meetings (27 of which were coauthored by students), and published 8 articles in refereed journals. Another 11 articles (2 of which have a student co-author) were accepted for publication or are in press, and 6 manuscripts (2 of which are co-authored by students) were submitted to peer-reviewed journals and are currently being reviewed. Through its research activities in living marine resources the Center is addressing NOAA Fisheries mission goal, to: “protect, restore, and manage the use of coastal and ocean resources through an Ecosystem Approach to Management”.

A total of $7,909,478 was collectively awarded in grants which directly impacted Center activities. These funds enhanced LMRCSC research through support of its faculty and students and by

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development/enhancement of infrastructure. Seventeen (17) grant proposals totaling $13,639,060 were submitted to various funding agencies during the current reporting period. In support of Goal 3, to generate a pool of scholars entering the field of marine science, 94 students (65 BS, 24 MS and 5 Ph.D) were supported and trained in NOAA core sciences during this reporting period, and 21 students graduated (20 BS and 1 MS). Seventy five K-12 students (66 middle school and 9 high school) received support through LMRCSC as part of their involvement in LMRCSC initiatives. To enhance the practical education of students in the marine sciences, faculty conducted research funded by LMRCSC that involved both graduate and undergraduate students as active research participants. During this reporting period, students participated in research projects at their home institutions, NOAA laboratories, and at partner institutions. These efforts provided opportunities for students to network with and gain experience from outstanding fisheries scientists. Students also benefited from their participation in the projects through hands-on research experience, completion of theses in partial fulfillment of their degree requirements, by receiving stipends and through travel awards to attend conferences. The Center also conducted K-12 educational activities including the SSU Marine Science Camp, which reached 86 students, the South Florida Shark Program at RSMAS that involved over 200 students from three local high schools and the Upward Bound Marine and Estuarine Science Program at UMES that reached 25 students. LMRCSC at UMES also hosted 28 Washington, DC area high school students from the NOAA and DC Educators Moving Ocean Science Forward (NEMO) program on June 21 and 22, 2007.

Two graduate students, Daniel Cullen (UMES) and Ebony Henderson (SSU) received training at NOAA Northeast Fisheries Science Center, Woods Hole, MA and the Southwest Fisheries Science Center, Galveston, TX, respectively. Furthermore, the Center provided scientific crew for a research cruise aboard the R/V Albatross IV in August, 2007, designed to assess the fisheries data provided by the new NOAA vessel, the R/V Bigelow. This cruise provided training to ten students from LMRCSC (UMES and HU), ISET (U of Alaska Southeast), the University of Arkansas Pine Bluff, and the University of Delaware, and a unique recruitment opportunity for the Center. Jose Reyes-Tomassini (COMB) has been awarded a NOAA-EPP Graduate Sciences Program fellowship beginning October 1, 2007 and has received a competitive small award from the Marine, Estuarine and Environmental Science program to bridge his funding from LMRCSC-COMB to the NOAA position. He will be continuing his behavioral analysis with Dr. Barry Berejikian at the NOAA Northwest Fisheries Science Center in Seattle, Washington.

The foregoing suggest that the LMRCSC educational, research and outreach activities are addressing three of the five essential activities NOAA has identified as being important for the success of its mission: (i) “developing, valuing, and sustaining a world-class workforce”, (ii) “ensuring sound, state-of-the-art research”, and (iii) “promoting environmental literacy”. Summary of the Performance Metrics for the period of March 1, 2007 to August 31, 2007 Performance Metrics Number a. # Students Trained with NOAA-related Science Curriculum 94 b. # of students who graduated in NOAA core science areas 21 c. # of collaborative research projects with NOAA & other partners 11 d. # of peer reviewed publications in NOAA-related science 8 e. # of peer reviewed publications with students as co-authors 2 f. Amount of external funds awarded to LMRCSC $7,909,478

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INTRODUCTION

Established in October 2001, the Living Marine Resources Cooperative Science Center (LMRCSC) was created as a cooperative agreement between NOAA Educational Partnership Program, the University of Maryland Eastern Shore (UMES), Delaware State University (DSU), Hampton University (HU), Savannah State University (SSU), the University of Miami (UM/RSMAS) and the University of Maryland Biotechnology Institute Center of Marine Biotechnology (COMB). UMES is the lead institution. The mission of the Center is to conduct research congruent with the interests of NOAA Fisheries and to prepare students for careers in research, management, and public policy that support the sustainable harvest and conservation of our nation's living marine resources. After the first five years of funding, the LMRCSC successfully competed for a new center award for the period of 2006 to 2011. The following are the goals and objectives of the LMRCSC: Goal 1: Enhance Marine Research and Academic Program Capacities of Center MSIs Goal 1 is accomplished through the following objective:

Objective 1: Build upon Existing Academic and Organizational Infrastructure to Develop

Strong Interactive Academic Programs in Marine Sciences

Goal 2: Enhance Research Programs in the Marine Sciences at Participating Institutions Goal 2 is accomplished through the following objectives:

Objective 1: Continue Current and Develop New Research Programs Congruent with NOAA

Fisheries Research Priorities Objective 2: Fully Integrate Research Programs with NOAA Fisheries to Ensure Long Term

Funding and Programmatic Stability Goal 3: Generate a Pool of Scholars Entering the Field of Marine Sciences and Fisheries Goal 3 is accomplished by the following objectives:

Objective 1: Enhance Recruitment of Students from Underrepresented Groups into Marine Science Disciplines

Objective 2: Strengthen Outreach & Education Programs to Students and the Public Objective 3: Develop Programs that Enhance the Practical Education of Undergraduate and

Graduate Students in the Marine Sciences SECTION I – STATUS OF AWARD ACTIVITIES (Goals and Objectives) For the period March 1 to August 31, 2007, the following tasks were accomplished in support of the goals and objectives of the LMRCSC:

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Goal 1: Enhance Marine Research and Academic Program Capacities of Center MSIs

Objective 1: Build upon Existing Academic and Organizational Infrastructure to Develop Strong Interactive Academic Programs in Marine Sciences

In the current reporting period, the Center institutions achieved this objective through: i. Increased use of the Virtual Campus for a) curriculum development and b) meetings, ii. Strengthening collaborations among center partners, and iii. Strengthening administrative and programmatic components of the Center i) Increased use of the Virtual Campus Spring Seminar Series The Virtual Campus continues to be an effective tool for conducting seminars, meetings and courses at the Center. The Center’s Spring 2007 Seminar Series (Table 1) coordinated by Dr. Joseph Love (UMES) was a cooperative effort between UMES faculty and faculty at the University of Arkansas Pine Bluff, and featured guest speakers from various universities and agencies. DSU and RSMAS students and faculty used IVN to participate in the seminar series. Table 1. LMRCSC Spring 2007 Seminar Series Date Seminar Name Speaker Affiliate Institution

Feb 13 Production of the saltwater baitfish Fundulus heteroclitus to offset wild harvest Dennis McIntosh Delaware State

University

Feb 20 American shad restoration on the Roanoke River, North Carolina Kevin Dockendorf

North Carolina Wildlife Resources Commission

Mar 6 Sustainable Paddlefish Production Strategies Steven Mims Kentucky State University

Mar 13 Murky Waters: Environmental defense fund revisited. Eric May UMES

Mar 27 Supplemental Stocking of Largemouth Bass in a Kentucky Reservoir Ryan Oster Kentucky State

University Apr 3 Conservation Genetics Matt Powell University of Idaho Apr 10 Columbia River Salmonid Supplementation Christine Moffitt University of Idaho

Apr 17 Aquaculture contribution to public stock enhancement in Virginia Brian Nerrie Virginia State

University

Apr 24 Genetic Resources and Genetic Improvement: is aquaculture solving problems or causing them? Jeff Silverstein USDA West Virginia

May 1 Supplementation of Red Drum in the Gulf of Mexico with hatchery fish David Abrego Texas Parks and

Wildlife Department

COMB has also contributed to the use of the virtual campus through provision and partial funding of a seminar series on Harmful Algal Blooms which were made available to LMRCSC partners.

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Table 2. Harmful Algal Bloom Seminar Series at COMB Date Speaker/Inst Topic

5/16/07 Dr. Andrew E. Allen, J. Craig Venter Institute

The Multi-Lineage Richness of Diatom Genomes and Transcriptional Responses to Nutrient Stress

5/23/07 Dr. Tsvetan R. Bachvaroff UMBI-COMB

Genomic Arrangement of Dinoflagellate Genes: From Stop to Start

5/30/07 Kristy Lidie, Marine Biotoxins Group/NOAA, Hollins Marine Labs

Life Without Nucleosomes: Transcript Processing and Post-transcriptional Gene Regulation in the Dinoflagellate Karenia brevis

6/27/07 Dr. Gustaaf M. Hallegraeff, University of Tasmania, Hobart, Australia

Harmful Algal Blooms: From Molecular Processes to Global Ocean Environment

6/27/07 Dr. Brett Neilan, The University of New South Wales, Sydney Australia

The Evolution and Regulation of Small Molecule Biosynthesis in Toxic Cyanobacteria

ia) Academic Courses: Several University of Maryland system MEES courses offered in Spring 2007 (Table 3) and Fall 2007 (Table 4) were also made available to Center students via IVN. UMES graduate students Dan Cullen and Lonnie Gonsalves enrolled in the Bioenergetics and Population Dynamics course taught at Chesapeake Biological Laboratory at Solomons, MD in Fall 2007. That semester, Dan Cullen also enrolled in Quantitative Methods taught at Chesapeake Biological Laboratory (CBL). SSU, HU, RSMAS and DSU students were not enrolled in MEES courses for credit during the reporting period. The Center is developing an Articulation Agreement among these partner institutions and the MEES system to make it possible for students at these non-University System of Maryland institutions to enroll in the courses via IVN. Table 3. Courses Offered in Spring Semester 2007 Using Interactive Video Network (IVN). MEES 498B Biometry MEES 608D Scientific Writing & Communication MEES 608L Molecular Microbial Ecology MEES 608X Advanced Topics in Aquatic Ecology MEES 608Y Topics in Wildlife & Conservation Ecology MEES 627 Environmental Geochemistry MEES 661 Physics of Marine and Estuarine Environments MEES 698A Aquatic Microbial Ecology Table 4. Courses Offered in Fall Semester 2007 Using Interactive Video Network (IVN). MEES 698F Biology of Fishes MEES 634 Bioenergetics & Population Dynamics MEES 621 Biological Oceanography MEES Sustainable Aquaculture

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MEES 607 Quantitative Methods MEES 698E Zooplankton Ecology

The courses listed in Tables 3 and 4 above address various aspects of the study of living marine resources and their environments, hence, they were offered to students to strengthen their backgrounds in the LMRCSC thematic areas: Quantitative fisheries, Essential Fish Habitat, and Aquaculture. The Scientific Writing and Communication course was designed to prepare students to be able to present their research results to the scientific community and the public effectively. LMRCSC students are not required to enroll in all the courses listed above, but instead they take only the course(s) that they need for graduation which are not offered at their home institutions. In addition to the above courses, the following course is being taught at UMES in Fall 2007 via the Virtual Campus. Ichthyology: Through the use of the Virtual Campus, students at Savannah State University have been able to enroll in Dr. Joseph Love’s (UMES) course in Ichthyology (BIOL 688B) for the Fall semester, 2007. Eight students at UMES and ten from SSU are participating in the class which meets on Tuesdays and Thursdays from 9:30 to 10:50am. MEES 608i Fisheries Policy & Management: An Ecosystems Approach to Fisheries Management: Like the Fisheries Policy and Management course originally taught in Spring, 2005, a team of fisheries management experts from NOAA and other agencies has been assembled by Dr. Eric May at UMES and Kevin Chu of the NOAA Northeast Regional Office to offer this course via the Virtual Campus to students at all Center partners for the Fall semester, 2007. The first class will be held on September 12, 2007. ib) Meetings. Apart from instruction, the Virtual Campus was used for Executive Committee meetings of the LMRCSC on May 21, 2007, July 16, 2007 and August 14, 2007. An additional Executive Committee meeting was held via teleconference call on March 26, 2007. The Virtual Campus was also used on August 13 to include Dr. Andrea Johnson, who was collaborating on a research project at the Northeast Fisheries Science Center, in a discussion of mutual research interests with colleagues from the Chesapeake Biological Laboratory (University of Maryland Center for Environmental Science) and LMRCSC. iii. Strengthening administrative and programmatic components of the Center The Center continued to be guided by two management tracks, A) Administrative and B) Programmatic. A. The Administrative component includes the Center Director, Program Manager, Executive

Committee, Center Core Administration and the Board of Visitors. Center Director and Staff No staffing changes were made during this reporting period. However, during this period, DSU took over the salary obligations for Dr. Gulnihal Ozbay who was supported by the LMRCSC. DSU is currently undergoing the internal hiring process to fill the vacant LMRCSC faculty line which will further strengthen capacity at the institution. Executive Committee (EC): The EC consists of the Distinguished Research Scientist, Project Directors from each Center institution and Center Director; Dr. Chigbu serves as chair of the EC. The EC met via the

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Virtual Campus on May 21, 2007, July 16, 2007 and August 14, 2007. An additional Executive Committee meeting was held via teleconference call on March 26, 2007. Center Core Administration (CCA): The CCA, consisting of UMES’ Vice-President for Academic Affairs, a representative from the office of the President, UMES’ Dean of the School of Agriculture and Natural Sciences, Chair of Department of Natural Sciences, LMRCSC Director, Distinguished Research Scientist, and Program Manager, did not meet during this reporting period. B. Programmatic: Technical Advisory Board (TAB): The LMRCSC Project Directors decided strategically to not have Cooperative Workshops this year. Instead, we used the research concepts in the four LMRCSC research thematic areas (Quantitative Fisheries, Essential Fish Habitat, Aquaculture and Fisheries Socioeconomics) developed during the 2005 collaborative workshops, as the basis for developing the 2007 Request for Proposals (RFP). The Cooperative Workshops held in 2004 and 2005 clearly identified priority areas for research congruent with the mission of NOAA NMFS, however they were somewhat redundant, with little inter-annual change in priorities. In 2006 and continuing in 2007 travel restrictions due to continuing resolutions reduced the availability of NOAA NMFS scientists that could attend conferences and workshops of this nature. It was decided in April of 2007 to use an alternate paradigm aimed at 1) providing to investigators a more detailed RFP such that proposals are more in line with the intent of NOAA NMFS priorities previously identified, 2) avoiding redundancy in results that were seen between 2004 and 2005, 3) fostering stronger collaboration with NOAA NMFS and between partner institutions. To this end, individual conferences were held through teleconferencing that addressed the four thematic areas. In the case of socioeconomics, individual communication with scientists from Silver Springs, MD and Woods Hole, MA was held and a draft RFP was prepared and submitted to NMFS scientists for review and comment prior to publication. For aquaculture, both Sea Grant and NOAA Aquaculture Matrix scientists held a teleconference to discuss priorities and research needs. Following meetings with NOAA NMFS scientists, phone conferences were held with division and section heads at the NMFS Science Centers to refine the RFP and identify NOAA NMFS scientists that would serve as contacts for partner institution scientists to work with in developing proposals for submission. This process required extensive efforts from the Distinguished Research Scientist and was felt to be much more cost effective than annual workshops. Workshops will continue to be an important aspect of developing research priorities, but will be held biannually rather than yearly and used to refine the current RFP which is considered as a living document. The annual LMRCSC Request for Proposals was distributed to Center researchers and their collaborators on August 8, 2007. Proposals for the 2007/2008 year are due to Program Manager, Todd Christenson by 5:00PM on October 12, 2007. The LMRCSC Technical Advisory Board, which will review the proposals, is comprised of the following individuals:

Dr. Bonnie Ponwith (Deputy Director, Office of Science & Technology, NMFS) Dr. Tom Noji (Director, J.J. Howard Marine Science Lab., Sandy Hook, NJ) Dr. Ed Houde (Chesapeake Biological Lab.) Dr. Bob Iwamoto (NOAA NWFSC, Seattle, WA) Dr. Phil Logan (NOAA NEFSC, Woods Hole, MA) Dr. Richard Brill (VIMS) Dr. James Berkson (NOAA SEFSC, Virginia Tech) NOAA EPP Representative (as an observer)

The LMRCSC technical representative serves as the Chair of the Board.

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Monitoring and Evaluation LMRCSC Exit Evaluation: An exit evaluation form has been created and is being distributed to graduating students. To date, nine students at UMES, both undergraduate and graduate have completed and submitted this form. The students had many positive things to say about the LMRCSC at UMES which are summarized in Appendix I. Nevertheless, some suggestions were made for improving the program. We have made or initiated changes to improve the quality of the program that we offer since some of the student’s reviews were made. Presented below are the students’ suggestions and an account of how each of the comments has been or is being addressed. Suggestions on improvement of LMRCSC from Student Exit Evaluations

1. More recognition of program from UMES: o Meetings have been held on several occasions between the LMRCSC Director and the

administration at UMES, including President Dr. Thelma B. Thompson, to discuss the LMRCSC program and its importance to the University. President Thompson presided over the LMRCSC Board of Visitors meeting held October 22, 2007.

o The LMRCSC newsletter, The Living Sea, is distributed to the offices of the President, Vice-Presidents for Academic Affairs and Administrative Affairs, and Dean of the School of Agriculture and Natural Sciences.

o LMRCSC Seminars are open to the campus community and are publicized to students and faculty within the Department of Natural Sciences.

o LMRCSC faculty teach many courses at UMES providing them with opportunities to publicize the Center and its activities as well as recruit students into the program.

o UMES publicists are invited to cover LMRCSC sponsored events such as the Upward Bound Marine and Estuarine Science Program for UMES publications such as The Key.

o A student Chapter of the American Fisheries Society has been formed at UMES. o LMRCSC students at UMES will hold their 1st graduate student Symposium on February

15, 2008 which will bring together faculty and students in the School of Agriculture and Natural Sciences.

2. Increase diversity of faculty/staff:

a. LMRCSC adheres to the hiring policies of UMES, which is an Equal Employment Opportunity/Affirmative Action employer. LMRCSC has faculty with diverse backgrounds in the marine sciences and fisheries.

3. Increase mentoring of students:

a. LMRCSC requires its graduate students at UMES to serve at least one semester as teaching assistants for courses offered in the Department of Natural Sciences. Many serve in this capacity for additional semesters.

b. LMRCSC graduate students at UMES are expected to provide tutoring services to students enrolled in undergraduate classes in the Department of Natural Sciences.

4. Increase communication between students from LMRCSC partners:

a. Communication among students from Center partners is facilitated by their interactions at the following events:

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i. Meetings such as the American Fisheries Society, which is well-attended by LMRCSC students.

ii. Symposia such as the Mid-Atlantic HBCU Research Day. This event is held annually at UMES. Students from UMES, DSU and HU have participated each of the last two years.

5. Raise standards for graduate students, especially PhDs. 4 years is too long for an MS

degree: a. LMRCSC takes the academic performance of its students very seriously. At UMES, like all

Center partners, LMRCSC students adhere to the following Special Award Conditions as set by NOAA for Award #NA06OAR4810163:

i. EPP funds may not be used to support students who do not meet the minimum 3.0 Grade Point Average.

ii. EPP funds for direct student support shall be provided for no more than five (5) years for students pursuing doctorate degrees, providing the student maintains the minimum of 3.0 Grade Point Average.

iii. EPP funds for direct student support shall be provided for no more than five (3) years for students pursuing master’s degrees, providing the student maintains the minimum of 3.0 Grade Point Average

b. LMRCSC Graduate students and their academic advisors at UMES are now required to submit to the Center Director milestones for graduate students to follow to ensure timely completion of their degree programs.

6. Advise students early regarding entry requirements for the Marine, Estuarine and

Environmental Science (MEES) program at UMES: a. LMRCSC undergraduate students who intend to continue at UMES under the MEES

Master of Science program are advised early regarding the academic requirements of the program.

b. Students participating in the 5 year BS/MS program at UMES typically complete some of the first year M.S. requirements during their final year in the B.S. program.

c. Because most LMRCSC graduate students enter UMES under the MEES program, prospective LMRCSC recruits are advised extensively by Program Manager Todd Christenson to ensure that the application process proceeds smoothly, that required documents are submitted and deadlines are met.

7. Improve research opportunities at UMES to avoid having to go elsewhere to conduct

research: a. Since the advent of the LMRCSC TAB and the RFP process in 2004, 6-8 collaborative

research projects have been funded by LMRCSC each year in which UMES researchers have served as PI or Co-PI. These projects as well as many others are conducted on UMES campus. Graduate and undergraduate students have played an active role in conducting the research.

b. The following research facilities are now available at UMES which have been utilized by LMRCSC students:

i. Coastal Ecology Teaching and Research Center: This 10,000 square foot facility, located at Assateague Island, houses a microbiology lab, a large general teaching lab, a wet lab, age and growth lab, storage for biological collections, auditorium

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and office space. The facility is equipped with nets, multiparameter water quality meters, microscopes, pH meters, autoclave, spectrophotometer. The laboratory has been used extensively for research projects on larval ingression and recruitment and diets of coastal fishes, and for classes and K-12 activities.

ii. Fish physiology lab: The lab established using leveraged funds from Title III is equipped with centrifuges, a fume hood, freezers, incubators, age and growth facilities, blood analyzer, and a high grade teaching microscope. This lab has been used extensively during Dr. Johnson’s project “Biology of large monkfish” and for her Independent Study and Undergraduate Research classes.

iii. Aquaculture facility: UMES aquaculture building is equipped to conduct medium to large scale studies on captive fish.

iv. ICP/MS: This facility was utilized by LMRCSC graduate Ai’sha Washington in her research on cardiomyopathy in pygmy sperm whales.

8. Create small publication of key things for students to know prior to starting internship:

a. LMRCSC acknowledges the value of this suggestion and is currently preparing a publication which will be available before summer, 2008.

9. More cross training between labs at UMES:

a. The involvement of additional faculty at UMES in LMRCSC sponsored projects (Dr. Ali Ishaque, Dr. Joseph Pitula and Dr. Yan Waguespack) has provided opportunities for students to receive additional training in analytical techniques (e.g. molecular biology, analytical chemistry and environmental toxicology) applicable to fisheries research.

Online Evaluation Forms: Online forms for evaluation of various aspects of the LMRCSC Program are available through SSU’s Survey Research Center (http://www.srcssu.com/noaa/). These forms became functional on May 1, 2005 and are directly linked to the LMRCSC home page. To date, 16 students have provided evaluations of their internship experiences at various NOAA facilities, Gray’s Reef National Marine Sanctuary, SSU and COMB. Similarly, 9 supervisors have provided evaluations of the LMRCSC students who participated in internships in their labs. The data provided will assist the Center to ensure the best possible internship experiences for both students and their supervisors. Goal 2: Enhance Research Programs in the Marine Sciences at Participating Institutions Objective 1: Continue Current and Develop New Research Programs Congruent with NOAA Fisheries Research Priorities The LMRCSC has completed the initial draft of the center-wide research project, “Ecological Studies of Black Sea Bass in the Atlantic and Gulf of Mexico Coastal Waters” which will examine the population dynamics and ecology of black seabass (Centropristis striata) along the Atlantic coast of the United States from Florida to New Jersey. The draft has been submitted to the partner institutions, and is currently under review. A meeting will be held over the next reporting period to flesh out projects which will be integrated into the final version.

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The proposed research on black sea bass is based on the Atlantic coastal distribution of the species, its recreational and commercial importance, position as a keystone species in reef and structured bottom habitats, and priorities set forward by NOAA NMFS for this species. Black sea bass distribution ranges from Massachusetts to Florida and thus represents a species that would be of interest to all partner institutions and their stakeholders involved in management of marine fish species. The proposal has as its goals: 1) To Establish a Quantitative Relationship between Post-Settlement Larval and 1st Year Post-Winter Juvenile Abundance and Adult Atlantic Stock Recruitment; 2) Determine Along a Broad Spatial and Temporal Scale if Key Life History Parameters and Biological Reference Points Vary and 3) Establish On a Broad Spatial Scale Relationships Between Population Structure and Available Habitats. These goals were designed to engage all partners on subprojects that would be directed towards addressing each as well as meet the mission and goals articulated in the NOAA Strategic Plan (2006) which are to:

• Protect, Restore, and Manage the Use of Coastal and Ocean Resources Through an Ecosystem Approach to Management and

• Provide Critical Support for NOAA’s Mission This project supports the mission and goals of NMFS by addressing its Ecosystems Observation components which include: marine resource surveys, habitat assessments, commercial fisheries statistics, and marine recreational fisheries statistics. The current issues related to this species are that there are several data gaps that are needed before a full Ecosystem Based Fisheries Management Plan can be put into place. These include:

1. Examination of hydrographic mechanism and larval behaviors controlling transport from spawning sites to settlement habitats including inter-annual variations,

2. Determination of mechanisms that control migration from offshore areas to estuarine and near-shore nursery grounds,

3. Relational studies on habitat quality of coastal ocean and estuarine nursery grounds, and

4. Studies on all aspects of reproductive biology and behavior The LMRCSC also continues to work with Elizabeth City State University scientists to develop projects looking at the evolution of coupled human and natural systems. Dr. Anne Garland and Dr. Eric May submitted an abstract for a paper to be presented at the SHA 2008 Conference on Historical and Underwater Archaeology Albuquerque, New Mexico January 9-12, 2008 entitled “Public Archaeology in Historical Ecology”. In addition, plans are being made to develop a course at UMES examining the role of the sociological sciences in fisheries and ecosystem management. RSMAS graduate students and LMRCSC associated faculty funded by the LMRCSC continued their research work in the areas of essential fish habitat, aquaculture, fishery socio-economics and quantitative fisheries. Aquaculture research conducted during this period was done on the context of a cobia research project funded by the TAB and explained in detail below. The work in essential fish habitat was conducted by graduate students Xaymara Serrano and Dwight Ebanks. Xaymara is a M.S. student working on the physiological definition of habitat constraints for grey snapper in South Florida. Xaymara collaborates in her project with Dr. Martin Grosell from RSMAS and Dr. Joe Seraffy from NMFS SEFSC. During this period Xaymara completed most of her field data collection and much of the laboratory experiments. Dwight Ebanks is a PhD student who spent this period

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developing his research proposal in collaboration with Dr. Nelson Ehrhardt (RSMAS) and making contacts with the NEFSC lab scientists to seek their collaboration in his proposed research on essential fish habitat of coastal Atlantic rays. Dwight also made a visit to Delaware State University during this period in order to explore the possible collaboration of Dr. Dwayne Fox in his project. Research in Socio-economics was conducted by Ayeisha Brinson on the Atlantic billfish fisheries in collaboration with Dr. David Die (RSMAS) and Dr. Juan Agar (NMFS/SEFSC). During this period Ayeisha completed her data collection with a visit to Senegal where she collected data on the recreational fisheries for sailfish that occur in this country. In Senegal, Ayeisha was hosted by research scientists from the National Fisheries laboratory and by officers of the Senegalese sport fisheries federation. Quantitative fisheries is the main area of research of Dr. David Die (RSMAS) who during this period focused on work to support the assessment of tropical shared resources harvested by artisanal fleets. In June and July 2007, Dr. Die led two workshops, one in Senegal, organized by ICCAT to improve fishery statistics on highly migratory fish and scientific capacity in countries of West Africa and a workshop in St. Vincent, organized by the CRFM that focused on the assessment of coastal pelagics and reef fish. During the rest of the period Dr. Die continued his work directing the Center for Independent Experts that provides NMFS with independent peer review of science products. Additionally, during April 2007, Dr. Die was one of the co-organizers of the SEFSC workshop on CPUE standardization that grouped NOAA and RSMAS Scientists in discussions on this topic. Research at HU is limited primarily to the TAB funded “Water Quality Monitoring of Hampton River” which is used primarily to train undergraduate students. HU currently does not support graduate students in the LMRCSC. TAB Approved Projects for Year 1 (2006/2007) The following 11 projects were approved for funding by the LMRCSC Technical Advisory Board (TAB) on December 18, 2006 (Table 5) and are currently underway. A no-cost extension for these projects was granted by LMRCSC Director Paulinus Chigbu until March 31, 2008 to allow the work to be completed. The delay in completing the projects stemmed in part from the fact that because of the uncertainty of receiving funds from NOAA for a new center in 2006, the LMRCSC RFP process and TAB review of proposals were not completed until December 18, 2006. Hence, a few more months were needed beyond the reporting date of August 31, 2007 for the funded projects to have duration of 12 months. Table 5. LMRCSC Projects Approved by Technical Advisory Board for 2006/2007.

2006/2007 LMRCSC Approved Projects Investigators Title Direct Cost Carla Curran, SSU Paul Pennington, NOAA

Effect of isopod parasite Probopyrus pandalicola on the grass shrimp Palaeomonetes pugio: Impacts on population density, gravidity, and tolerance to contaminants

$31,417.00

Dewayne Fox, DSU Brad Wetherbee, URI Nancy Kohler, NOAA Camilla McCandless, NOAA

Habitat use, depth selection & timing of residency for sand tiger sharks (Carcharias taurus) in DE Bay

$40,384.00

Ali Ishaque, UMES Yan Waguespack, UMES Chris Chambers, NOAA

Evaluation of Interactive effects of contaminants on full life cycle responses for assessment of habitat quality

$45,007.00

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Andrea Johnson, UMES Anne Richards, NOAA

Biology of large monkfish, Lophius americanus

$40,950.00

Joseph Love, UMES Gretchen Martin, NOAA Seasonal differences in larval Ingression through Ocean City Inlet (Maryland)

$21,739.00

Refik, RSMAS Daniel Benetti, RSMAS Rosemary Jagus, COMB

Evaluation of adaptation to stress conditions in cobia larvae and juveniles cultured with and without the use of probiotics $41,667.00

Eric Schott, COMB Rosemary Jagus, COMB Joseph Pitula, UMES Dionne Hoskins, SSU

Development of strain and species-specific probes to investigate reservoirs and genetic diversity of the blue crab parasite Hematodinium spp.

$49,748.00

Yossi Tal, COMB Yonathan Zohar, COMB Daniel Benetti, RSMAS Rosemary Jagus, COMB

Evaluation of cobia (Rachycentron canadum) production in a fully contained, environmentally sustainable recirculating aquaculture system.

$48,728.00

John Trant, COMB Andrea Johnson, UMES Evaluation of chicken Litter as a Feminizing Agent in Striped Bass

$21,466.00

Yan Waguespack, UMES Andrew Draxler, NOAA Ashok Deshpande, NOAA

Experimental assessment of the effects of contaminated sediments from the Lower Hudson-Raritan estuary and Elizabeth River on Winter Flounder

$45,200.00

Eric Wooden, HU Kenneth Moore, NOAA

Water quality monitoring in the Hampton River

$23,075.00

TAB Approved Projects Year 1 Status

The 11 TAB approved projects for year 1 of the new center grant, which are currently underway, are described below. Other projects in support of undergraduate or graduate involvement in research are reported in Appendix II. 1) Project Title: Effect of isopod parasite Probopyrus pandalicola on the grass shrimp Palaeomonetes pugio: Impacts on population density, gravidity, and tolerance to contaminants Project Description: Palaemonetes pugio is a food source for numerous commercially important species. Habitat quality is in part based on food availability and can be determined indirectly by assessing fish growth (Curran and Able 2002; DuBeck and Curran, in review). As grass shrimp are an abundant macrofaunal species, their density can play an important role in determining habitat quality and therefore whether areas could be essential fish habitats. The main objective of this work is to determine the effect that the isopod parasite Probopyrus pandalicola has on grass shrimp density (since they prevent reproduction) and construct a population model to quantify this impact. Results will be disseminated to local schools through the funded participants, including a teacher-intern who will design a K-12 activity. Thematic Area Addressed: Essential Habitat Lead Scientist(s): Dr. Mary Carla Curran (COMB) NOAA Collaborator(s): Dr. Paul Pennington (NOAA) LMRCSC Collaborator(s): Dr. Dionne Hoskins (NOAA Fisheries/Savannah State University) LMRCSC Research Student(s): Michael Partridge, (graduate student, SSU), Chris Williamson, undergraduate student, Amber Stanley (undergraduate student, SSU), Jennifer Kinney (undergraduate student), and Terry Aultman (teacher-intern) External Collaborator(s): Elizabeth King, College of Charleston

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Planned Start Date: Fall 2005 Planned End Date: Sept 2007 Actual Start Date: 1 Oct 2005 Actual End Date: 30 Sept 2007 Planned and actual results of project: One of the objectives was to determine the effect that the bopyrid isopod has on grass shrimp density and construct a population model to quantify this impact. In addition, in year 1 studies were conducted to determine whether multistressors (e.g., parasite infection and contamination load) had an additive effect on shrimp mortality in the laboratory. Due to student interest year 1 efforts focused on the laboratory studies. Michael Partridge (SSU Graduate Student) is conducting thesis research on the effect of the parasite on swimming endurance in the grass shrimp. To date no effect of the parasite on shrimp endurance has been demonstrated, although there seems to be a direct relationship between endurance and shrimp size. Mr. Partridge will continue research on the effect of resmethrin/Scourge on swimming endurance this fall. Mr. Partridge will defend his Master’s thesis in March 2008. Chris Williamson (SSU Undergraduate Student) collaborated with the NOAA PI (Paul Pennington) in the summer of 2007. The work determined that the toxicity of resmethrin/Scourge was not higher in parasitized vs. unparasitized grass shrimp as determined using an LC50. This work was more productive than anticipated and was extended to conduct a toxicity test using another insecticide, bifenthrin. The results for the LC50 ratio test were that the effect of bifenthrin on parasitized when compared to non-parasitized P. pugio was significantly different at 24 hours (P = 0.0065). It is anticipated that these results will be submitted for publication in a peer-reviewed journal this fall. Work has begun on the field assessment/ shrimp population model and, in year 2, efforts will focus on this aspect of the project. To date, shrimp densities were found to not exceed 40.8 shrimp/m3 at Country Club Creek, but values reached 145.6/ m3 at Moon River. The overall percent parasitism rate was low (<1%); however, 56% of the females were gravid. Population information will continue to be conducted to better elucidate the impact of the parasite on the shrimp population, as parasitism prevents reproduction in P. pugio. The results of this project include: one Master’s thesis proposal entitled “The Effect of the Parasite Probopyrus pandalicola on the Swimming Endurance of the Grass Shrimp Palaemonetes pugio,” one manuscript that is nearly ready to submit entitled “The Toxicity of Synthetic Pyrethroid Insecticides on the Grass Shrimp, Palaemonetes pugio Parasitized with the Bopyrid Isopod, Probopyrus pandalicola,” and one K-12 activity submitted for publication entitled “Grass Shrimp: Small Size But Big Role in Food Web.” Two other undergraduate students completed small projects and assisted with field sampling. To date, the research has been presented as 3 posters at 2 different conferences. How will results be incorporated into NOAA Fisheries operations? Results from this research are relevant to the management, conservation, and protection of living marine resources, all of which are part of the NOAA mission. The significance of grass shrimp as an important food resource for many commercial species has been demonstrated and this resource can be affected by insecticides. In some cases, sensitivity to pesticides is greater when the shrimp is parasitized by a bopyrid isopod. In addition, this

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isopod prevents the shrimp from reproducing, which in turn can affect shrimp populations and thereby impact habitat quality. How will results be incorporated into LMRCSC research and curriculum? This funded research is an integral part of SSU’s contribution to LMRCSC. Dr. Curran’s teacher intern is incorporating her findings into K-12 curricula, just as past LMRCSC teacher interns have done in her laboratory. Dr. Curran is incorporating her field experience into her classroom teachings, and both she and her students have spoken at local schools. 2. Project Title: Habitat use, depth selection, and the timing of residency for Sand tiger sharks (Carcharias taurus) in Delaware Bay Project Description: Both juvenile and adult sand tiger shark (Carcharias taurus) commonly occur in Delaware Bay. Like many North American shark species, sand tiger shark populations have declined dramatically, likely due to a combination of overharvest and life history traits (e.g. late maturity and low fecundity). As a result, sand tiger shark was among those petitioned and added to the Candidate Species List under the Endangered Species Act in the fall of 1997. Previous work on sand tiger shark has suggested that protection of juveniles is of premium importance for enhanced recovery of stocks along the US Atlantic coast and that a fundamental component of such protection is the identification and protection of essential habitat for sand tiger shark. However, information on the location of discrete nurseries, fine-scale and seasonal movement patterns and other essential habitat for all segments of the population for this species along the US Atlantic coast is limited and fragmentary. Through this proposal researchers utilize both passive and acoustic telemetry to collect information on habitat utilization, depth selection and residency patterns for sand tiger shark in Delaware Bay. The information gathered through this project will provide resource managers much needed information as they begin to formulate a recovery plan for this important resource. Additionally, this project will allow participation of both graduate and undergraduate students in a program of study (essential fisheries habitat) identified as a targeted research area within the LMRCSC. These students will have an opportunity to gain first hand knowledge of some of the methodologies used to assess essential fish habitat and to contribute to the wise utilization of an abundant and ecologically important species within the local marine environment. Thematic Area Addressed: Quantitative Fisheries and Essential Fish Habitat Lead Scientist(s): Dr. Dewayne Fox (DSU), NOAA Collaborator(s): Drs. Nancy Kohler and Cami McCandless (NMFS Narragansett, Rhode Island, Apex Predator Program) LMRCSC Collaborator(s): N/A LMRCSC Research Student(s): Mr. Johnny Moore (formerly undergraduate at Savannah State) who is now taking his final three courses at DSU before applying for acceptance into the DSU MS program (expected Winter 08). Mr. Moore arrived in Delaware and started his field work in June 07. External Collaborator(s): Dr. Brad Wetherbee (University of Rhode Island). Planned Start Date: October 1, 2006 Planned End Date: August 30, 2008

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Actual Start Date: October 1, 2006 Actual End Date: To be determined- a request for year two funding will be submitted for LMRCSC TAB research funding Planned and actual results: Sampling for this project began on June 4th and continued for the duration of this reporting cycle. Information gleaned by the return of two telemetered sand tiger shark which were tagged during July, 2006 indicated a return to Delaware Bay during the 3rd week of June, 2007. This corresponds to the first successful capture of sand tiger shark. Over the course of the 2007 summer field season all transmitters (n=25) were successfully implanted into both juvenile and adult sand tiger sharks. Telemetry results indicate that sand tiger sharks occupy a greater extent of Delaware Bay than previously thought. All telemetered sharks were relocated either through passive detections or manual tracking following release. To date, in excess of 45,000 detections have been recorded in the passive listening array. These data represent a huge increase in the amount of information available for managers as they try to understand this protected resource’s habitat needs. How will results be incorporated into NOAA Fisheries operations? The overall goals of this project are to provide information useful for enhancement of the recovery efforts for a depleted marine resource, provide information useful for development of measures to protect sand tiger sharks and their essential nursery habitat, allow collection of baseline information for understanding impacts of habitat changes on spatial and temporal use of these areas, and improved understanding of the role of this key predator within the bay. How will results be incorporated into LMRCSC research and curriculum? This project will provide a direct benefit to the residents of Delaware and the Mid-Atlantic region through a number of components. These include education via public lectures and classroom visits as well as incorporating the work into existing active outreach and educational programs at the Delaware National Estuarine Research Reserve (DENERR). Specifically, this work will be presented through visual displays and graphic computer models depicting fine scale and long term movement patterns and habitat utilization of sand tiger sharks in DE Bay. The DENERR has an estuarine education component but lacks the essential information on much of the fish component of Delaware Bay. As one of the largest sharks to commonly inhabit Delaware Bay the sand tiger shark will serve as an ideal gateway species to get the K-12 segment of our population interested in the successful stewardship of our marine environment, which is in line with the goals of NOAA Fisheries. Finally, this project will allow participation of both graduate and undergraduate minority students in a program of study (essential fisheries habitat) identified as a targeted research area within the LMRCSC. These students will have an opportunity to gain first hand knowledge of some of the methodologies used to assess essential fish habitat and to contribute to the wise utilization of an abundant and ecologically important species within the local marine environment. 3) Project Title: Evaluation of interactive effects of contaminants on full life cycle responses for

assessing fish habitat quality in estuaries Project Description: Inshore habitats are used by a variety of marine and estuarine organisms as a source of prey, location for reproduction, and shelter during vulnerable life-stages. These habitats are also the principal location for the infusion of contaminants into the marine environment. Understanding the extent and nature of contamination along industrialized watersheds, as well as the complexity of potential toxic responses by impacted organisms, remains a significant challenge to researchers and resource managers. The researchers are conducting an experimental analysis of the toxic response in a common fish of estuarine waters of the northeastern USA. The intent is to make substantial progress on this front by 1)

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examining the interactive and additive effects of a combination of ecologically relevant contaminants on an indicator fish species, 2) broadening the set of response variables collected previously to include characteristics of juveniles and of reproductive performance by adults, and 3) evaluating the relative merit of each response variable in the broadened data set in the context of their utility as a bioindicator. Thematic Area Addressed: Essential Fish Habitat Lead Scientist(s): Dr. Ali Ishaque (UMES); Dr. Yan Waguespack (UMES) NOAA Collaborator(s): Dr. Chris Chambers (James J. Howard Marine Sciences Laboratory, NOAA/NMFS/NEFSC, Highlands, New Jersey) LMRCSC Collaborator(s): None LMRCSC Research Student(s): Interviewed Undergraduate Student, Jeremiah Hicks (UMES) for summer internship and he will be offered an internship for the summer of 2007 to participate in the project. Planned Start Date: 12/01/06 Planned End Date: 11/30/08 Actual Start Date: 12/01/06 Actual End Date: To be determined Planned and actual results of project: An experimental laboratory approach is being used to assess full life cycle responses in Atlantic tomcod (Microgradus tomcod). This is an important member of the Raritan Bay / New York Harbor fish community as well as a proven research model. To date, the investigators on this project have: 1) Developed protocols and exposed embryos and young larvae of two populations of Atlantic tomcod to a

range of doses of PAHs and an Aroclor mixture of PCBs administered through aqueous solution. 2) Quantified the toxic responses expressed in early survival, age, size, shape, and condition at hatching,

and resistance of larvae to starvation. 3) Developed protocols for the use of a food-based vehicle for exposing larvae and juveniles to

subsequent doses of Aroclor PCBs. 4) Developed protocols for marking juvenile tomcod that allows us to pool treatment combinations in order

to rear tomcod to an advanced stage (reproductive maturation) but still allowing the investigators to maintain the treatment history for individual fish.

How will results be incorporated into NOAA Fisheries operations? These studies will provide first-of-their-kind evaluations of the interactive effects of multiple toxins on early life stages of a common estuarine fish of the northeastern USA. Moreover, the quantification and comparisons of multiple lethal and sublethal responses should have utility for identifying appropriate bioindicators in other ecotoxicological studies using fishes. How will results be incorporated into LMRCSC research and curriculum? The protocols that are developed regarding aqueous and trophic exposures for fish, and the quantification of multiple ecologically relevant response variables, will have broad applicability to other ecotoxicological studies on aquatic and marine organisms. It is expected that the skills being developed by interns and students will not only enhance their education, but result in these individuals being well situated for placement in their working careers.

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4) Project Title: The biology of large monkfish, Lophius americanus Project Description: The American monkfish or goosefish (Lophius americanus; Valenciennes) is a fast growing, benthic species belonging to the family Lophiidae (Steimle et al., 1999). It is found in the northwest Atlantic Ocean from the Grand Banks and northern Gulf of St. Lawrence, Canada to the east coast of Florida (DNR, 1999; NEFMC/MAFMC, 1998). Although the species ranges from Canada to Florida, it is most commonly found north of Cape Hatteras, North Carolina (Robbins et al., 1986) in shallow coastal waters to over 800 m (Stevens, 2004). Once considered ‘trash fish’ by the groundfish and scallop fisheries, monkfish has become an important commercial species. The US stocks have been overfished, and few large monkfish are now found in commercial landings or in Northeast Fisheries Science Center (NEFSC) annual bottom trawl surveys (NEFSC 2005). Although life history information is available for smaller monkfish, very little is known about the biology of monkfish greater than 90cm. The purpose of this study, therefore, was to: 1) estimate growth rates of large monkfish; 2) investigate reproductive biology of large monkfish and 3) estimate cannibalism rates of large monkfish. Thematic Area Addressed: Quantitative Fisheries Lead Scientist(s): Dr. Andrea Johnson (UMES) NOAA Collaborator(s): Dr. Anne Richards (Northeast Fisheries Science Center, Woods Hole) LMRCSC Collaborator(s): none LMRCSC Research Student(s): Dan Cullen, (graduate, UMES); Abram Townsend (Undergraduate, UMES), Belita Nguluwe (Undergraduate, UMES) Industry collaborators: John Stolgitis (Point Judith, RI), Chris Hickman (Chincoteague, VA and Cape Hatteras, NC) and Roger Wooleyhan (Ocean City, MD), Ernie Panacek (Barnegat Light, NJ), Chris Walker (Chincoteague, VA), Joe Kelly (Chincoteague, VA) and other gillnetters. Planned Start Date: January 1, 2007 Planned End Date: December 31, 2007 Actual Start Date: January 4, 2007 Actual End Date: To be determined Planned and actual results of project: It was proposed to collect monkfish, 90 cm and larger from January to December, 2007 from commercial gillnetters to: 1) determine the full growth curve and provide critical information on growth, natural mortality, longevity, and potential yield; 2) determine spawning frequency of these large monkfish to determine population productivity and resilience; and 3) estimate the rates of cannibalism by large monkfish to improve population models and fishery management. Since January, 2007, approximately 650 monkfish samples from fishing areas east of Gloucester, MA, to areas off Ocean City, MD and Chincoteague, VA have been collected. Collecting, processing and analyzing data from this study is continuing. To date, over 180 monkfish have been aged using both vertebrae and illicia and 383 gonads have been processed for histology. Of the 598 fish that have been processed, approximately 5% cannibalism has been observed. Fish Collection and Processing: A total of 598 monkfish were collected by commercial gillnetters in waters off Cape Ann, Massachusetts to Chincoteague, Virginia between January 2006 and July 2007.

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These sampling sites encompass the two management areas for monkfish, the Northern Fishery Management Area (NFMA, northern flank of Georges Bank and the Gulf of Maine) and the Southern Fishery Management Area (SFMA, southern flank of Georges Bank and Mid-Atlantic Bight south to Cape Hatteras, NC). Fish were processed in the laboratory. Individuals were weighed (kg) and measured (total length (TL) to the nearest 0.5 cm) and samples collected for studies of growth, food habits and reproductive biology. Age and Growth: Vertebrae were processed following the procedures of Armstrong et al. (1992) and Hartley (1995). Vertebra number 8 was baked for 1-1.5 hr at 230oC to enhance the contrast in presumed annual rings before counts were made using a dissecting microscope at 60x. All vertebrae were read by 2 or 3 independent readers and agreement between two readers was required for acceptance of an age estimate. Vertebrae were classified as “unreadable” if all readings differed. Reproduction: All samples (n = 383 females) were examined histologically by light microscopy and assigned maturity stages following the criteria of Martinez (1999). Female stages [perinucleolar, cortical alveoli, vitellogenic and final stage of oocyte maturation (FOM, hydrated)] were based on the maturity of the most advanced stage oocytes. Gonads and livers were weighed to calculate the gonadosomatic index (GSI) and the hepato-somatic index (HSI): GSI=100WG (W-WG) ¯1 HSI = 100WH (W-WH) ¯1 where WG= gonad weight WH = liver weight W= body weight Food Habits: Stomachs were removed and contents weighed. Prey items were sorted, identified to the lowest taxon possible, enumerated, weighed by prey category, and measured if possible. State of digestion of prey items was classified as fresh, partially digested or well digested. Intact monkfish prey were measured, weighed, and dissected to determine gender. The percent frequency of occurrence was calculated for major taxonomic prey groups and rate of cannibalism was determined for monkfish at each macroscopic gonadal stage (developing, ripe and spent). Statistical Analyses: An analysis of variance (ANOVA) was used to test the influence of month and gonadal stage on GSI and HSI. The Statistical Analysis System (SAS; SAS Institute Inc, Cary, NC) was used for all analyses. A visual assessment of residual plots was used to determine homogeneity of variance. To satisfy homogeneity of variance, HSI and GSI were natural log transformed. Significant ANOVAs (P < 0.05) were followed by Tukey’s multiple comparison tests (Neter et al. 1996). RESULTS All monkfish collected were females ranging in size from 69 to 118 cm, with a modal size of 91 cm. Most fish (>70%) were collected during February and from April to June, with very few fish collected the rest of the year because of the seasonality in fishing effort. Approximately 95% of the monkfish were collected from the southern management region extending from Buzzards Bay, MA to waters off Chincoteague, VA. The majority of fish (>60%) collected during this study came from waters off Pt. Judith, RI and off Ocean City, MD.

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Age and Growth: To date, age has been determined for 180 fish ranging in size from 69 to 118 cm that were collected during April-November 2006. These fish ranged in age from 8 to 13 years. The average annual increment between age classes was 8.8 cm, but these increments were variable. The greatest increase in growth appears to occur during the 2nd and 3rd quarters of the year; almost no increase in size at age was observed for samples taken during the 4th quarter. Reproduction: Hepato-somatic and gonado-somatic indices were determined for 419 monkfish, and gonadal histology for 383 monkfish. Mean HSI was significantly higher for February than all other months (P < 0.005) except January and July. Mean GSI values peaked during February through April with another peak in June. The June peak in GSI represents samples collected in waters off New York, Rhode Island and Massachusetts. The variance in GSI values increased in April because the percent of females in post-spawning condition increased from 16% (March) to 55%. This was also similar for females caught in June, where 78% were post-spawning females with atretic oocytes in their gonads. Females with hydrated oocytes (FOM) were found from January to June and August but most were caught in February, April and June. Comparisons of GSI and HSI to gonadal histology in females showed mean HSI was highest in females with vitellogenic oocytes, while GSI peaked during final oocyte maturation. Mean HSI values were significantly higher in females with vitellogenic and hydrated oocytes than those with cortical alveoli (P < 0.0001) and atretic oocytes (P = 0.0018 and P < 0.0001 respectively) whereas mean GSI were significantly different at each stage of gonadal development (P < 0.001). Food Habits: Of the 598 stomachs examined during the two years of this study, 65.9% were empty, 34.1% contained prey remains (including monkfish), and 5.5% contained remains of monkfish. Skate, monkfish and mackerel were the most frequent identifiable prey items. Evidence of cannibalism ranged from jaw bones to partially digested monkfish. Gender of monkfish prey was identifiable in two prey specimens, one female and the other male. The incidence of prey items was lowest in post-spawning monkfish (spent or resting). Gonadal stage did not influence the frequency of monkfish in the diet. How will results be incorporated into NOAA Fisheries operations? The results may be used to help improve several aspects of the population assessment for monkfish, including: (1) estimates of growth rates for monkfish, which are currently insufficient for determining asymptotic length due to a lack of large aged specimens, (2) cannibalism rates found in this study are much higher than in smaller monkfish, this will be incorporated in modeling studies and may have implications for optimal harvesting strategies, (3) so far, the study on using illicia instead of vertebrae for estimating age of monkfish, needs some work but this work could lead to significant improvements in commercial sampling for monkfish. Samples of vertebrae (the structure currently used for aging) are difficult to obtain because they damage the product, and commercial sampling is weak because of this. How will results be incorporated into LMRCSC research and curriculum? This study has provided training in monkfish biology and biological sampling for several undergraduates and a graduate student (Daniel Cullen) who has spent time working at the NEFSC, Woods Hole, MA studying different aspects of monkfish biology and learning various fisheries techniques such as fish dissection, processing tissue samples for histology, analyzing gut contents, identifying parasites in tissues and organs and aging monkfish using both illicium and vertebra. Dan Cullen will present the results of this study at the American Fisheries Society in San Francisco in September, 2007.

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Funds for the second year of this study have been leveraged from NMFS Research Set-Aside (RSA) Program for 2007 (starting May 1, 2007). This program has provided the investigators with days-at-sea (DAS). The landings from these research DAS have provided funds to purchase additional monkfish for this study and allowed investigators to expand the study to include areas from the Gulf of Maine to Chincoteague, VA. 5) Project Title: Seasonal Differences in Larval Ingress through Ocean City Inlet (Maryland). Project Description: Larval fish ingress into coastal bays of Maryland will be monitored using methods used by others along the Atlantic coast of the U.S. to provide NOAA with details on larval fish recruitment. Larval recruitment into estuaries may prove to be an indicator of spawning stock biomass or large scale climatic patterns. Since December, 2004, students have been trained in field ecology and larval fish identification, and have established a long-term monitoring site. It is proposed to continue weekly sampling of Ocean City Inlet (December–July) to: 1) asses temporal stability of assemblage patterns; 2) construct habitat models for species that are abundant and management targets (e.g., summer flounder); and 3) determine if larval density and body condition are related to the abundance of zooplankton prey. Thematic Area Addressed: Essential Fish Habitat Quantitative Fisheries Lead Scientist(s): Dr. Joseph W. Love (UMES) NOAA Collaborator(s): Dr. Gretchen Bath-Martin (NOAA Beaufort Lab) LMRCSC Collaborator(s): None LMRCSC Research Student(s): Daniel Luers (Graduate, UMES), Greg Oliver (Undergraduate, UMES) Planned Start Date: 01/01/07 Planned End Date: 01/01/08 Actual Start Date: 01/01/07 Actual End Date: To be determined Planned and actual results of project: This research, with funding mainly from the TAB, has been conducted for the past 3 years. To date, the larval ingress patterns of 9 larval fish species entering Chincoteague Bay (the largest of Maryland’s coastal bays) for winter and spring seasons (Fig. A) as been documented. By far, the dominant larval species is American eel, Anguilla rostrata. This research has been presented at the tidewater chapter meeting of the American Fisheries Society (by Branson D. Williams, undergraduate), NOAA Environmental Partnership Program meeting (by Gregory L. Oliver, undergraduate), and a manuscript for peer-reviewed publication is in preparation. Most specimens have been preserved and retained to the LMRCSC Reference Collection at UMES (www.umes.edu/lmrcsc) and UMES Coastal Ecology Teaching and Research Center (CETRC) for teaching, learning, and outreach purposes. Throughout the study, five students (1 graduate, 4 undergraduate) have been trained in grant writing, field sampling, larval fish identification, data entry, and data analysis. Samples have also been used for training students in Ichthyology (BIOL 488F/688B) and for educating the public (via posters and events co-sponsored by LMRCSC, the National Aquarium in Baltimore and Maryland Coastal Bays Program). Investigators have continued their relationship with Drs. Gretchen Bath Martin (NOAA Beaufort Laboratory) and Kenneth Able (Rutgers University Marine Field Station). A partnership with Dr. Timothy Targett (University of Delaware) has been developed and this will assist in future funding from alternative sources (e.g., Maryland Sea Grant, National Science Foundation).

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The research that was funded by the TAB developed into two additional projects. First, Branson D. Williams organized and conducted research that: 1) resulted in an aging technique for larval American eels using pigmentation patterns; and 2) indicated longer migration times of American eel through wetlands. The latter research was presented at the international meeting of the Society of Wetland Scientists in Cairns, Australia (summer, 2006). The second research project was a pilot study of larval fishes entering the bays during summer, 2006. This preliminary research has since resulted in the collection of a diverse and very different assemblage of larval fishes entering the bays of Maryland. During the first year of the study, the objective of conducting a baseline survey of ichthyoplankton and quantifying the level of difference in larval ingress from the southern, Chincoteague Inlet and northern, Ocean City Inlet was met. When including only fish larvae, multivariate and univariate tests indicate little difference between the inlets with respect to the species composition and densities. The second objective was also met; which was to relate species densities and distribution to time, wind speed, wind direction, current speed, current direction and other environmental variables. There were distinct changes in assemblage composition as water temperatures warmed. However, more data are needed to draw reliable inferences. During the second year of research, survey efforts were continued and extended into July, this time sampling weekly and focusing research only at Ocean City Inlet. The analyses suggest that there are strong seasonal ingress patterns, even though sampling was mainly during winter and spring. Estimates of American eel density were refined and a lunar periodicity to ingress documented. Moreover, summer flounder (Paralichthys dentatus) was collected, which were not collected during the first year, indicating a need for multi-year studies. Finally, during the third year of study, Daniel Luers began working on this Master’s thesis research on ingress of American eel at UMES conducting sampling during day and night and weekly to better model ingress patterns of American eel. He is also investigating settlement patterns and growth of American eel larvae. How will results be incorporated into NOAA Fisheries operations? The data will generate an inventory of larval fish recruiting to lagoons in the Mid-Atlantic Region. While larval ingress work has been conducted at southern (North Carolina) and northern (New Jersey) locations, no complimentary work has been conducted for coastal lagoons that occur between these states. How will results be incorporated into LMRCSC research and curriculum? Both specimens and data are used to train students in taxonomy and statistics. Courses in both Ichthyology and Advanced Ecological Statistics have utilized specimens and data from this research project. This program has also helped establish a research network among scientists in the Mid-Atlantic Region and in the training of undergraduate and graduate students. 6) Project Title: Evaluation of adaptation to stress conditions in cobia larvae cultured with and without the use of probiotics Project Description: This project is aimed at developing and evaluating a new tool, based on the phosphorylation state of the eukaryotic initiation factor eIF2 which will assess the response of cobia larvae to eukaryotic pathogens. The project will entail the identification of common eukaryotic pathogens infecting cobia larvae under normal conditions of culture, and through challenge studies determine the response of the eIF2 to varying concentrations of the pathogens in the water.

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Thematic Area Addressed: Aquaculture Lead Scientist(s): Dr. Daniel Benetti (RSMAS) NOAA Collaborator(s): Dr. John Lamkin (NOAA-NMFS Southeast Fisheries Science Center) LMRCSC Collaborator(s): Dr. Rosemary Jagus (COMB) LMRCSC Research Student(s): Donald Bacoat (Graduate student, University of Miami, RSMAS-MAF) External Collaborator(s): Dr. Philippe Douillet (Ecomicrobials, LLC., and Adjunct professor, University of Miami, RSMAS-MAF) Planned Start Date: 01/01/2006 Planned End Date: 12/31/2007 Actual Start Date: 01/01/2006 Actual End Date: To be determined Planned and Actual Results of Project: Mr. Donald Bacoat (graduate student at RSMAS) worked in Dr. Jagus’ laboratory at COMB for a week in October 2006 and was able to demonstrate that eIF2α levels could be successfully monitored in both larvae and organs from Cobia juveniles. The important pathogens affecting hatchery reared cobia larvae and juveniles were identified as: Amyloodinium sp., Broklynella sp. and Cryptocarion sp. and the bacteria Photobacterium dansela - previously known as Pasteurella piscicida). Prophilactic treatments (formalin and hygrogen peroxide) are being used to prevent disease outbreaks and probiotics. This has been successful, having brought up survival rates to 15-35% this year from 1-5% in previous years. How will results be incorporated into NOAA Fisheries operations? The results from this study will shed light on how to reduce the mortality rate of cultured cobia and other cultured fish. This will directly result in an increased efficiency of rearing fish and relieve pressures on wild stocks of fish which are overfished. Furthermore, the results of this study will help increase our understanding of the use of probiotics so that the aquaculture industry can become less dependent on antibiotics to increase its yield of fish. Clearly, these results will aid in the ecosystem based management of fisheries which is a part of NOAA’s goals. How will results be incorporated into LMRCSC research and curriculum? In addition to aiding the ecosystem based management of fisheries, this research provided training to a student (Don Bacoat) in the NOAA-LMRCSC program. Don was taught how to conduct aquaculture research in an environmentally friendly manner and is becoming more and more prepared for a career in the marine sciences as well as a potential employee in NOAA’s developing marine aquaculture management program. Don is making progress towards graduating with an MS degree conducting research on probiotics and prophylactic treatments for cobia (anticipated graduation is spring 2008). 7) Project Title: Development of strain- and species-specific probes to investigate reservoirs and genetic diversity of the blue crab parasite Hematodinium sp. Project Description: Hematodinium sp. is a crustacean parasite associated with fishery losses of the blue crab (Callinectes sapidus) along the Atlantic and Gulf coasts. The commercial and recreational blue crab fishery is of critical economic and societal importance, yet there are insufficient tools to predict the

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occurrence and severity of Hematodinium sp. outbreaks. New and sensitive PCR-based assays will be applied to detect reservoirs of the parasite in crabs, prey species and the environment, thus addressing the question of parasite overwintering. A new parasite DNA sequence will be obtained from the ribosomal RNA genes, which will be used to develop a genetic ‘fingerprint’ for strains of Hematodinium sp. from different geographic areas. Together, these tools will allow tracking of Hematodinium sp. strains through time and space.

The project combines the efforts of scientists at five institutions in a project that will provide students a multidisciplinary experience that ranges from field collection of biological samples and water quality data to bioinformatics analysis of DNA sequence data. Each student participant will work with faculty at two institutions, either COMB/UMES or SSU/COMB. Also participating will be scientists at the Cooperative Oxford Lab (NOAA-NCCOS) and the Smithsonian Environmental Research Center. Thematic Area Addressed: Essential Fish Habitat Lead Scientist(s): Dr. Eric J Schott (COMB) NOAA Collaborator(s): Dr. Gretchen Messick (NCCOS, Cooperative Oxford Lab) LMRCSC Collaborator(s): Dr. Joseph Pitula (UMES), Dr. Dionne Hoskins (SSU), Dr. Rosemary Jagus (COMB) LMRCSC Research Student(s): Kimberly Williams (UMES), Ammar Hanif (Morgan State University) Planned Start Date: June 15, 2007 Planned End Date: June 14, 2008 Actual Start Date: June 15, 2007 Actual End Date: To be determined Planned and actual results of project: Blue crabs were sampled from the Pocomoke River in June and again in September with sediments and prey take. DNA is being extracted from samples, and Q-PCR will be conducted at SSU or COMB, to determine the pathogen load. Samples that test positive for Hematodinium sp. are to be further analyzed at COMB, by amplification of additional DNA sequence from the ribosomal RNA genes. Laboratory space at COMB is being provided by Dr. Rosemary Jagus, who also contributes to mentoring students that work with Dr. Schott. The dataset of rRNA gene sequences from samples originating in Chesapeake Bay, MD coastal bays, and GA coastal bays will be analyzed for polymorphisms that correlate with geographic region.

At SSU, crab samples are already archived from collections in 2005-2006. In June and September 2007, a student under the guidance of Dr. Hoskins is continuing to collect following samples from Wassaw Sound, GA: crabs, potential prey (primarily bivalves), and sediment. The student from SSU traveled to COMB for the summer to extract DNA and conduct Q-PCR to measure prevalence of Hematodinium sp. How will results be incorporated into NOAA Fisheries operations? The goal is to provide general information on how outbreaks of the parasite arise, whether through crab-to-crab transmission, via an intermediate host that is also a crab prey, or via environmental reservoirs- chiefly sediment. The technology used in this study can also be incorporated into ongoing yearly surveys of crab health. Q-PCR assays are already being applied to annual crab health surveys conducted by G. Messick at COL, with the expectation that it will become a standard part of Hematodinium sp. forecasting.

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COMB is a member of the Blue Crab Advanced Research Consortium (BCARC), a six-center, multi-year initiative, funded by NOAA-Fisheries, to explore the feasibility of hatchery-based broodstock enhancement of blue crabs in the Chesapeake Bay. The BCARC hatchery program at COMB needs to have accurate habitat assessments for planning releases of hatchery-reared juveniles. The data obtained by this LMRCSC study will indicate whether pathogens are present in potential habitat, and whether ongoing surveys of sediment will be necessary in the future crab restoration programs.

A related major question is whether strains of Hematodinium from one region are distinguishable from those in another region. Depending on the primary source of year-to-year infection, and whether the strains are genetically-distinguishable, managers may recommend actions to limit the spread of a given Hematodinium strain. How will results be incorporated into LMRCSC research and curriculum? The introduction of the Q-PCR assay to the suite of tools to monitor crabs, their prey, and habitat will broaden students’ perspective and tie together the field-based and lab-based investigations into a holistic approach to understanding disease prevalence. Students who understand the fundamentals of Q-PCR will also be prepared to conduct and interpret other molecular studies. The application of a molecular approach to the full life cycle of a parasite emphasizes the need to take a systems approach to ecological studies. 8) Project Title: Evaluation of cobia (Rachycentron canadum) production in a fully contained, environmentally sustainable recirculating marine aquaculture system. Project Description: Cobia (Rachycentron canadum) is an emerging aquaculture candidate for both offshore and land-based marine recirculating aquaculture system (RAS). The current study evaluates the possibility of culturing this new candidate species in a fully contained marine RAS developed by COMB scientists. The novel marine RAS includes an anaerobic water treatment loop that enables the collection of organic waste from the fish culture tank and uses it to support the microbial process for nitrate reduction. It is believed that such systems will be used both for growing fish to market size, as well as in concert with offshore mariculture through the production of juveniles (hatchery/nursery) or seed fish of a desired optimal size for stocking net-pens. Thematic Area Addressed: Aquaculture Lead Scientist(s): Dr. Yossi Tal (COMB) NOAA Collaborator(s): Dr. Walton Dickhoff (Northwest Fisheries Science Center, Seattle) LMRCSC Collaborator(s): Dr. Yonathan Zohar (COMB), Refik Orhun (RSMAS), Daniel Benetti (RSMAS) LMRCSC Research Student(s): Brandon Flowers, David Shelton (UMES) Planned Start Date: January 1, 2007 Planned End Date: December 31, 2007 Actual Start Date: January 1, 2007 Actual End Date: To be determined Planned and actual results of project: The project work plan is on schedule. COMB recirculating aquaculture system is currently upgraded to meet the requirements of the cobia fingerlings. These upgrades include better oxygen delivery and biofiltration systems. Two minority undergraduate students - Brandon Flowers and David Shelton, both from UMES - worked on this project as summer interns in 2007.

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How will results be incorporated into NOAA Fisheries operations? NOAA’s aquaculture program includes the development of sustainable, environmentally-friendly production methods for new species of commercially valuable marine fish. The current proposal goal is aligned with this mission. Cobia is an emerging aquaculture candidate for both offshore and land-based RAS systems. The COMB model system represents the new generation of marine RAS, which is completely contained with no or near-to-zero effect on the environment. The combination of a prime candidate species and innovative technology will undoubtedly produce a sustainable method for the production of this desirable new marine culture species. How will results be incorporated into LMRCSC research and curriculum? The underlying goal of this work is to provide training for two minority students in the state-of-the-art molecular methods to study microbial communities in marine biofilters, as well as the development of environmentally-responsible recirculating marine aquaculture systems technologies. The students will take an active part in experimental design, data processing and evaluation. Moreover, the students in this project will be required to read and study relevant scientific papers related to the project prior to their arrival at COMB. This training and experience as an active part in a scientific project will serve as an excellent stepping stone for their future successful careers as marine scientists, which is part of the mission of the LMRCSC program. The students will be required to present their findings at the COMB LMRCSC Student Research Day, on August 3 and to prepare a poster which they will be encouraged to present at relevant student research venues. If the students complete these assignments satisfactorily, they will be eligible to receive 3 credits at the discretion of their home institution. 9. Project Title: Evaluation of Chicken Litter as a Feminizing Agent in Striped Bass Project Description: Agricultural runoff is a major source of endocrine disrupting chemicals (EDC). Chicken litter is a potentially rich source of plant-derived estrogenic EDC (phytoestrogens) which have been shown to alter sex development in a variety of fish species. This project will evaluate the potency of a water soluble fraction of chicken litter (CLWSF) to impact sexual development in transgenic zebrafish in which the reporter gene, GFP has been placed behind the promoter for the GnRH1. This transgenic fish provides a tractable model organism for which a multitude of research tools are available, but will allow predictions on how chicken litter run-off might impact the spawning of striped bass in the Chesapeake Bay. Thematic Area Addressed: Essential Fish Habitat Lead Scientist(s): Dr. John Trant: (Note: Because of Dr. Trant's move to Associate VPAA position, Dr. Jagus will coordinate this project with Dr. Eytan Abraham & Dr. Yoni Zohar at COMB and Dr. Johnson at UMES) NOAA Collaborator(s): None LMRCSC Collaborator(s): Dr. Andrea Johnson (UMES) LMRCSC Research Student(s): Belita Nguluwe (Undergraduate, UMES) Planned Start Date: 01/01/07 Planned End Date: 12/31/07 Actual Start Date: 01/01/07 Actual End Date: To be determined Planned and actual results of project: Dr. Johnson provided the chicken litter water soluble fraction, collected from the UMES farm in June 2007. This project provided summer internships to Belita Nguluwe undergraduate from UMES who performed the tests on zebrafish during the summer, 2007. Data from

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these studies have yet to be analyzed and, therefore, no results are yet available. Belita presented her methodology at the COMB Research Day on August 3, 2007. How will results be incorporated into NOAA Fisheries operations? This proposal addresses the RFP’s targeted research area of Essential Fish Habitat and is relevant to NOAA’s mission of conserving and managing the coastal and marine resources to meet the economic needs of the Nation. Since over 70-90% of the striped bass population utilize the Chesapeake Bay and its tributaries for spawning and nursery grounds (Herbst 2002), the data collected in this study will greatly contribute to NOAA’s goals of assessing EFH of striped bass and other managed species. All data analyses will be performed by Drs. Jagus and Johnson and the results will be reported to NMFS, published in a peer-review journal and presented at scientific conferences. Thus, the results of this study will provide information that can be used to better manage the striped bass population and its estuarine spawning and nursery habitat, the Chesapeake Bay. How will results be incorporated into LMRCSC research and curriculum? The underlying educational goal of this work is to provide training for a minority undergraduate student in the state-of-the-art molecular methods to study fish. The student will take an active part in experimental design, data processing and evaluation. Moreover, the students in this project will be required to read and study relevant scientific papers related to the project prior to their arrival to COMB. This training and experience as an active part in a scientific project will serve as an excellent stepping stone for their future successful career as marine scientists that is part of the mission of the LMRCSC program. 10) Project Title: Experimental assessment of the effects of contaminated sediments from Lower Hudson-Raritan estuary and Elizabeth River on winter flounder” Project Description: Winter flounder is unique because it spawns in estuaries and produces adhesive eggs that sink. This creates the potential for embryonic exposure to toxic sediments, which can result in both lethal and sublethal effects. Previous work showed that laboratory-reared winter flounder had higher in situ mortality, and displayed a variety of sublethal (morphological, survival to various early-life stages) effects following an embryonic exposure to local Lower Hudson-Raritan estuary sediments. Groups of juvenile winter flounder were also exposed to varying concentrations of sediments from Lower Hudson-Raritan estuary and mixed in varying proportions (0, 25, 50, 75, 100%) with sediments from York River, Virginia for up to 60 days. Tissues of control and treatment groups of seven days exposure were analyzed for 20 PCB congeners and 16 chlorinated pesticides by GC-ECD. The mean concentrations were higher in the treated fish for more than 70% of these contaminants, suggesting some contaminant uptake. Plans are underway to analyze the winter flounder that were exposed to contaminated sediment over longer periods of time (30, 45, and 60 days). Lipid composition and fish size will be included in statistical analyses in order to evaluate the significance of their contribution to contaminant accumulation. Thematic Area Addressed: Essential Fish Habitat Lead Scientist(s): Dr. Yan Waguespack (UMES); Dr. Andrew Draxler (NOAA-Fisheries); Dr. Ashok Deshpande (NOAA-Fisheries) NOAA Collaborator(s): Dr. Andrew Draxler (NOAA-Fisheries); Dr. Ashok Deshpande (NOAA-Fisheries) LMRCSC Collaborator(s): None LMRCSC Research Student(s): Bashiru Balogun (Graduate student, UMES) Planned Start Date: 12/28/06 Planned End Date: 12/28/07

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Actual Start Date: 12/28/06 Actual End Date: To be determined Planned and actual results of project: For the last two years, this research has shown that laboratory-reared winter flounder had higher in situ mortality, and displayed a variety of sublethal (morphological, survival to various early-life stages) effects following an embryonic exposure to Lower Hudson-Raritan estuary sediments. Gender effects of pollutants have also been investigated. The histological analyses of the gonadal tissues showed that there was no effect on gender as a result of exposure of embryos to Lower Hudson-Raritan estuary sediments. Juvenile winter flounder have been subjected to concentration gradients of the contaminated sediments, and then assessed for lethal and sublethal responses, as well as the tissue burdens due to the exposure regimes. The seven-day exposure data suggested some contaminant uptake. There are plans to expand the analyses to groups of fish with longer exposure times (up to 60 days).

Chemical Analyses for uptake of PCBs and other organic compounds were conducted using gas chromatography with electron capture detection (GC-ECD). The tissues of 27 juvenile winter flounder were evaluated for each of the following four organic analytes that were detected by GC-ECD: mirex, BZ-153, BZ-138, and BZ-187. When exposed to both Lower Hudson-Raritan estuary and York River, Virginia sediments, the mean concentrations of the organics (mirex, BZ-138, BZ-153, and BZ-187) in fish tissue were higher than control. It appears that organic concentrations for 30 days exposure were higher than 60 days sediment exposure. It is possible that the saturation limit was reached after a shorter period of time. More analyses on sediment and fish samples are needed to verify the data.

In September 2007, a poster presentation is planned in Helsinki at the Annual Science Conference of the International Council for the Exploration of the Sea. The poster (Effects of sediment contaminants on winter flounder in estuaries along the east coast of the US by Wieczorek, D., A. F. J. Draxler, A. D. Deshpande, Y. Y. Waguespack, R. C. Chambers, and T. H. Cleary) will detail sub-lethal and lethal effect of anthropogenic compounds (including PCBs, PAHs and pesticides) observed on young-of-the-year winter flounder (Pseudopleuronectes americanus) that settle on contaminated sediments. How will results be incorporated into NOAA Fisheries operations? Winter flounder is a valuable commercial and recreational fish which needs increased protection because of over-harvest and damage to its habitat. To protect winter flounder, efforts need to be made not only on preventing over-harvest, but also on maintaining healthy habitat for the fish making this project consistent with NOAA’s mission. The project specifically recognizes the interrelated nature of coastal habitat quality and marine organism. It will provide NOAA with a better understanding on winter flounder. How will results be incorporated into LMRCSC research and curriculum? The chemical analysis of the juvenile winter flounder samples by GC-ECD will form part of Bashiru Balogun’s dissertation at UMES. The experimental techniques to be used for chemical analysis of the fish will offer a wide range of opportunities for LMRCSC researchers and students. 11) Project Title: Water Quality Monitoring of the Hampton River Project Description: Hampton University has a long-term monitoring program on the Hampton River that has been funded by LMRCSC since 2006. HU established this project to collect basic background information to support future fisheries research on the Hampton River. The river runs along the shore of the University, which makes it a convenient location for research for undergraduate students, who maintain

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the monitoring station. The system collects water and atmospheric data at 15-minute intervals. This monitoring program includes a citizen-monitoring program which yields weekly, one-point collections increasing the spatial data of the river and at the same time increases public awareness. This summer investigators completed a water quality display that featured the water quality project and the associated nekton projects that use the water quality data to support Hampton’s fisheries research. This year more resources are being applied to improve dissolved oxygen and chlorophyll data, as the river is eutrophic with high oxygen demands. Monitoring has allowed researchers to evaluate the health of the natural marine resources and keep informed of current conditions of the river to aid in conservation efforts of the Hampton River. Thematic Area Addressed: Essential Fish Habitat Lead Scientist(s): Eric Wooden (HU), Dr. George Burbanck (HU) NOAA Collaborator(s) (please include affiliation): Dr. Ken Moore (Chesapeake Bay National Estuarine Research Reserve in Virginia), CBNERRVA LMRCSC Research Student(s): Carissa Wilkerson (Hampton University) Robyn Day (Hampton University) Courtney McGeachy (Hampton University) Ashley Nance (Hampton University) Hamani Wilson (Hampton University) Planned Start Date: January 01, 2007 Planned End Date: December 31, 2007 Actual Start Date: January 01, 2007 Actual End Date: To be determined Planned and actual results of project: Hampton University is committed to developing research opportunities for our faculty and students. Developing a monitoring program allows Hampton University to expand its own research and create partnerships with local entities. Hampton University would like to have this water quality database to strengthen our fish habitat/ecology efforts and development of the Hampton River database. This project allows Hampton University and NOAA to develop a product that will directly address the water quality concerns and therefore habitat quality of marine life in the Hampton River, which is important to sustainable fisheries and human health. The station is currently collecting continuous water and atmospheric data. The goal is to maintain this status until December, 2007. Two citizen monitors are still in the program collecting data on a weekly basis. The students are maintaining the site. In January 2008, a report will be submitted on the 2007 data. How will results be incorporated into NOAA Fisheries operations? LMRCSC conducted a workshop in June 2005 to identify project areas of interest. In the essential fish habitat session, an influence of watersheds on marine communities was identified as a project area. Nutrient loadings and eutrophication were two specific issues along with development of appropriate monitoring schemes were suggested as areas needed to be addressed. This project is just one project which starts to address those questions and supports the on going habitat conservation efforts at HU which are also important to NOAA. We have a pilot beach seining survey that collects nekton weekly in the Hampton Roads. This is a nekton community

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study in which all species are counted and measured but the interest in the abundance of summer flounder. NOAA is supporting ecosystem-based fisheries management that factors all relevant environmental variables. The project will contribute to spatial and temporal distribution of nekton, characterization of habitats and distribution, trophic interactions, and habitat utilization. NOAA is interested in applying such information to management decisions. How will results be incorporated into LMRCSC research and curriculum? Hampton University supports LMRCSC’s efforts by conducting research and training students who have an interest in careers in marine science. The research will form the foundation of future collaborative research efforts with NOAA scientists and other LMRCSC partners. This project will stimulate students’ interest in marine science and provide them with skills which will prepare them for graduate school.

SCHOLARLY PRODUCTIVITY In the current reporting period, LMRCSC students and faculty made 37 presentations (oral and poster) at scientific meetings, published 8 articles in refereed journals with another 11 in press. In addition, 6 manuscripts have been submitted for publication. The articles presented, published, submitted and in preparation are listed below. The majority of the presentations and publications came from students and faculty at UMES, DSU, COMB, SSU and RSMAS. Few of the presentations came from HU and none of the published articles was from HU. Oral Presentations (*Students) Abraham, E., Palevich, O., Ijiri, S., Gothilf, Y. & Zohar, Y.: Ontogeny and manipulation of the fish GnRH-1

system using transgenic GnRH-1-GFP zebrafish, 8th International Marine Biotechnology Conference, Eilat Israel, March 2007.

Brady*, M.G., C. M. Cairns, and D. A. Fox. Non-native parasitic nematode infection rates of American eel in Delaware waters: a cause for concern? Honors Day. Delaware State University, Dover, Delaware. May, 2007.

Brady, M.G.*, C. M. Cairns, and D. A. Fox. Non-native parasitic nematode infection rates of American eel in Delaware waters: a cause for concern? International Conference on the Biology and Management of Diadromous Fishes. Halifax, NS. June, 2007.

Cairns, C.M. *, D. A. Fox, L. Bailey, K. Shertzer. Sustainability of American eel in the St. Jones River, DE: a collaborative approach. International Conference on the Biology and Management of Diadromous Fishes. Halifax, NS. June, 2007.

Die D.J. CIMAS, 2007. CUFER and the CIE. NOAA population dynamics fellows workshop. Miami, April 2007.

Die D.J. 2007. Using alternative variance/covariance matrices to account for correlations in time and space and to examine potential changes in fishing power. SEFSC workshop on the standardization of cpue. Miami April 2007.

Die D.J. and WJ. Walter. 2007. Quantifying annual variation in catchability. SEFSC workshop on the standardization of cpue. Miami, April 2007.

DuBeck, G.D. *, Pressler A*, Harris K.*, Curran M.C.. The use of a small tidal creek in Georgia, USA as a settlement or nursery area for flatfishes. July 2007; Larval Fish Conference St. John’s Newfoundland, Canada

Fox, D. A. The king is gone but not forgotten: plight of the Delaware River Atlantic sturgeon. University Seminar. University of Delaware College of Marine Studies. Lewes, Delaware. April 2007.

Joshi, B., Richardson, M. A. and Jagus, R.: Interferon-upregulated eIF2α-kinases in fish, 8th International

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Marine Biotechnology Conference, Eilat Israel, March 2007. Ozbay, G.. Bivalve Restoration, Bio-filtration and Water Quality. U.S. Army Corps of Engineers Meeting

with Delaware State University, Lincoln University, Morgan State University, Cheney University. Delaware State University, Dover, Delaware. June 15, 2007

Montalvo, N. F.* & Hill, R.: Cultured bacterial isolates from Xestospongia spp. sponges, 8th International Marine Biotechnology Conference, Eilat Israel, March 2007.

Reyes-Tomassini, J. J. and Zohar, Y.: Analysis of Dominance Hierarchies in Seabream, Sparus aurata, a Protandrous Hermaphrodite. Animal Behavior Society 44th Annual Meeting, Burlington, VT, July 2007.

Simpson, P.*, and D. A. Fox. Contemporary understanding of the Delaware River Atlantic sturgeon: survival in a highly impacted aquatic ecosystem. International Conference on the Biology and Management of Diadromous Fishes. Halifax, NS. June, 2007.

Tal, Y., Schreier, H. J, & Zohar, Y. A novel approach for the production of marine fish in a fully closed, environmentally sustainable, marine recirculating system, 8th International Marine Biotechnology Conference, Eilat Israel, March 2007.

Wetherbee, B.M.* and D. A. Fox. Site fidelity and patterns of habitat use of sand tiger sharks (Carcharias Taurus) in Delaware Bay. Joint Meeting of the American Society of Ichthyologists and Herpetologists and the American Elasmobranch Society. St. Louis, Missouri. July, 2007.

Willet, W.A. *, D. A. Fox, and B. M. Wetherbee. Monitoring site fidelity and habitat utilization of juvenile sandbar shark (Carcharhinus plumbeus) within essential nursery habitat of Delaware Bay. Joint Meeting of the American Society of Ichthyologists and Herpetologists and the American Elasmobranch Society. St. Louis, Missouri. July, 2007.

Poster presentations (*Students): Babineaux, C., Brubaker, J., and J. Vandever. Comparison of finite and deep-water wave models and York

River, VA. Fifteenth Annual Mid-Atlantic HBCU Science Research Day. University of Maryland Eastern Shore. April 12, 2007.

Chambliss, S.S.*, G. Ozbay, G.H. Wikfors and A. Place. Measuring the Internal pH of Prorocentrum minimum using Fluorescent Probe Carboxy SNARF-1. Fifteenth Annual Mid-Atlantic HBCU Science Research Day. University of Maryland Eastern Shore. April 12, 2007.

Davis, J.*, and J. O’Neil. Nitrogen fixation associated with macrophytes in the Chesapeake Bay region. Fifteenth Annual Mid-Atlantic HBCU Science Research Day. University of Maryland Eastern Shore. April 12, 2007.

Gonsalves, L*. Down by the riverside: Practical mechanisms for introducing students to fisheries science. Fifteenth Annual Mid-Atlantic HBCU Science Research Day. University of Maryland Eastern Shore. April 12, 2007.

Henderson, N.D.*, Cox, L., and R. Hannigan. Distinguishing between anthropogenic and biogenic organics in black shale draining streams. Fifteenth Annual Mid-Atlantic HBCU Science Research Day. University of Maryland Eastern Shore. April 12, 2007.

Hughes, J. L. and Ozbay, G. Comparisons of Prorocentrum minimum and Karlodinium veneficum Growth in Cell Culture Flasks of Varying Surface Areas under the Same Light Regime. Delaware State University, 34rd Annual Honors Day. March 28, 2007

Joshi, B. and Jagus, R.: Comparative analyses of eIF4E family members in fish, 8th International Marine Biotechnology Conference, Eilat Israel, March 2007.

Miller, J., T. West, K. Buzby, K. Semmens, A. Lazur and D. McIntosh. Use of Aquaponics as a secondary crop and effluent treatment in ponds, raceways and recirculating tank systems. American Society for Horticultural Science. Scottsdale, Arizona. July, 2007,

34

Nance, A.*, Hollowman, E., and M. Newman. Mercury exposure through seafood consumption: A probabilistic risk assessment. Fifteenth Annual Mid-Atlantic HBCU Science Research Day. University of Maryland Eastern Shore. April 12, 2007.

Newhard, J.J.*, J. Love, and J. Gill.. Spatial and temporal patterns of fish distribution in Blackwater River drainage. NCBO Fisheries Science Symposium, Laurel, MD. April 2007

Nguluwe, B.S.*, Johnson, A.K., Lang, K., and A. Richards. Comparison of two methods of aging monkfish: Vertebrae vs illicia. Fifteenth Annual Mid-Atlantic HBCU Science Research Day. University of Maryland Eastern Shore. April 12, 2007.

Oliver, G.* Diet and co-occurrence of smooth and spiny dogfish. Fifteenth Annual Mid-Atlantic HBCU Science Research Day. University of Maryland Eastern Shore. April 12, 2007.

Palevich, O., Abraham, E., Gothilf, Y. & Zohar, Y.: Molecular regulation of GnRH neural migration in zebrafish, 8th International Marine Biotechnology Conference, Eilat Israel, March 2007.

Patterson, R.* Plant propagation of submerged aquatic vegetation. Fifteenth Annual Mid-Atlantic HBCU Science Research Day. University of Maryland Eastern Shore. April 12, 2007.

Reyes-Tomassini, J. J.* and Zohar, Y.: Analysis of Dominance Hierarchies in Seabream, Sparus aurata, a Protandrous Hermaphrodite. American Fisheries Society 137th Meeting, San Francisco, CA, September 2007.

Robinson, S.O. *, M.C. Curran, D.L. Hoskins. Above and Belowground Biomass for Spartina alterniflora In Five Georgia Marsh Dieback Sites. Atlantic Estuarine Research Society (AERS) & Southeastern Estuarine Research Society (SEERS) Spring Meeting 15-17Mar07, Pine Knoll Shore, NC.

Schott, E. J., Jennings, K. Murphy, S., Nagle*, Pitula, J.S., L., Place, A. R., Messick, G. and Jagus, R.,: Application of quantitative PCR to monitor dynamics of the crab parasite hematodinium sp. in experimental and field studes of the blue crab, Calinectes sapidus.

Schott, E. J., Messick, G., & Place, A. R., Aspects of the Molecular Biology of the blue crab parasite, Hematodinium sp. 32nd Eastern Fish health Conference, Gettysburg, PA. June 19-21, 2007.

Speaks, C.M.*, Brill, R., and P. Bushnell. The effect of magnets on the feeding behavior of sandbar sharks (Carcharinus plumbeus). Fifteenth Annual Mid-Atlantic HBCU Science Research Day. University of Maryland Eastern Shore. April 12, 2007.

Taylor, D.L.*, Love, J.W., Johnson, A.K., and J. Shoop*. The composition and diversity of phytoplankton communities of the Chesapeake Bay watershed as related to the planktivorous Atlantic menhaden. Fifteenth Annual Mid-Atlantic HBCU Science Research Day. University of Maryland Eastern Shore. April 12, 2007.

Ukaegbu, O., McKenzie, J. F., and Ozbay, G. Water Quality in Relation to Freshwater Mussel Density (Elliptio complanata) and Aquaculture Pond Water Management. University of Delaware Summer Undergraduate Research Symposium, August 8, 2007

Williams, K.D.*, and J.S. Pitula. Cloning and enzymatic activity of an aconitase orthologue from Perkinsus marinus. Fifteenth Annual Mid-Atlantic HBCU Science Research Day. University of Maryland Eastern Shore. April 12, 2007.

Publications Published Chigbu, P. & Sobolev, D. (2007). Bacteriological Analysis of Water. In L.M.L. Nollet (editor). Handbook of

Water Analysis. Second Edition. Mercel Dekker, Inc., New York, Basel. Fagervold, S. K., May, H. D., and Sowers, K. R. (2007) Microbial reductive dechlorination of aroclor 1260

in Baltimore harbor sediment microcosms is catalyzed by three phylotypes within the phylum

35

Chloroflex, Appl. Environ. Microbiol. 73, 3009-18. Kan J, Suzuki MT, Wang K, Evans SE, Chen F. (2007). High Temporal but Low Spatial Heterogeneity of

Bacterioplankton in the Chesapeake Bay, Appl Environ Microbiol. Kleisner, K., J. Sladek Nowlis, D. Olson, and D. J. Die. (2007). An application of spatial modeling to the

classification of pelagic habitat and the subsequent estimation of temporal abundance of dolphinfish, Coryphaena hippurus, in the western Atlantic. Proceedings of the Third International Symposium on GIS/Spatial Analyses in Fishery and Aquatic Sciences (22–26 August 2005, Shanghai Fisheries University, Shanghai, China).

Love, J.W., and E.B. May, (2007) Relationships between fish assemblage structure and selected environmental factors in Maryland’s coastal bays. Northeastern Naturalist. 14: 251-268.

Montgomery, S.S., A. Courtney, C. Blount, D.J. Die, M. Cosgrove and J. Stewart. (2007). Patterns in the distribution and abundance of female eastern king prawns, Melicertus plebejus (Hess, 1865), capable of spawning and egg production in waters off eastern Australia . Fisheries Research. (In Press)

Ozbay, G. (2007). Comparison of Filtration of Various Size Oysters (Crassostrea virginica). Journal of Environmental Monitoring and Restoration. 3:59-73.

Rhoads, R. E., Dinkova, T. D., and Jagus, R. (2007): Approaches for Analyzing the Differential Activities and Functions of eIF4E Family Members, Methods in Enzymology, Mechanistic Studies of Eukaryotic Translation Initiation, Lorsch, J., ed, Academic Press, 429, 261-298.

Wang K, Chen F. (2007): Prevalence of highly host-specific cyanophages in the estuarine environment, Environ Microbiol.

Zmora, N., Trant, J., Chan, S. M., Chung, J. S. (2007). Vitellogenin and its messenger RNA during ovarian development in the female blue crab, Callinectes sapidus: gene expression, synthesis, transport, and cleavage, Biol Reprod. 77, 138-46.

In Press or Accepted Able, K. W., K. M. M. Jones and D. A. Fox. (2007). Large nektonic fishes in nearshore and marsh creek

habitats in the Delaware Bay estuary: distribution and abundance. Northeastern Naturalist. In Press.

Bergan, V., Jagus, R., Stensvik, S., Kileng, Ø., and Børre Robertsen (2007): The Atlantic salmon zDNA binding protein kinase (PKZ) phosphorylates eIF2 alpha and constitutes a unique orthologue to the mammalian dsRNA activated protein kinase R (PKR). FEBS Journal. In Press.

*Chaplin-Ebanks, S. A. and M.C. Curran. 2007. Prevalence of bopyrid isopod Probopyrus pandalicola in the grass shrimp Palaemonetes pugio in four tidal creeks on the South Carolina-Georgia Coast. Journal of Parasitology. In Press.

Chigbu, P., Jearld, A. & Fogarty, M. J. (2007). National Oceanic and Atmospheric Administration and Jackson State University Program in Fish Stock Assessment. Journal of Geoscience Education (In Press).

Jacobs, J. M., M. Rhodes, B. Martin, D. McIntosh, W. F. Van Heukelem and R. M. Harrell (2007). Tissue specific sampling for the estimation of proximate compositional components in Striped Bass (Morone saxatilis). Transactions of the American Fisheries Society. In Press.

Love, J.W. and P.D. Chase. (2007). Marine fish diversity and composition in the Atlantic and South Atlantic Bights. Southeastern Naturalist. In press.

36

Jackson, A.R., Rouse, D.R. and Ozbay, G. (2007). The Effects of Oyster (Crassostrea virginica) Structure and Bioactivity on Water Quality, Sediment Nutrients, Infaunal Communities, and Transient Fish Biomass. Journal of Environmental Monitoring and Restoration. In Press.

Ozbay, G., Jackson, M. and Munyei, N. (2007). Temperature- and Salinity-Dependent Phytoplankton Profiles for the St. Jones River Watershed, Delaware. Journal of Environmental Monitoring and Restoration, In Press.

Pride C. (2007). Journal of Geoscience Education (special issue on diversity), Accepted. Fogleman, T. and M.C. Curran (2007). Making and Measuring a Model of a Salt Marsh. NSTA (National

Science Teachers Association) Science Scope, Accepted. Submitted (*LMRCSC Student): Johnson, A.K., Richards, A., Cullen, D.W.*, Nguluwe, B.*, and K. Lang. The biology of large monkfish,

Lophius americanus. Proceedings of the International Conference for the Exploration of the Sea Annual Science Conference. Helsinki, Finland.

Love, J.W., Chigbu, P., Slacum, W. and E.B. May. Inter-annual climatic variation influences dispersal of transient coastal fish species: consequences to assemblage dynamics and ecosystem management. Transactions of the American Fisheries Society.

Love, J.W., Chigbu, P., and E.B. May. Climatic and environmental variability affect distributions of resident and transient coastal fish species (Maryland, USA). Submitted to North American Journal of Fisheries Management.

McIntosh, D. and J. Ewart. 2007 Aquaculture Situation and Outlook Report: Delaware. Northeast Regional Aquaculture Center.

McIntosh, D. Aquaculture Risk Management. Technical Report. Northeast Regional Aquaculture Center. Nagle, L., Place, A. R., Schott, E.J., Jagus, R., Messick, G. and J.S. Pitula. Real-time Quantitative PCR-

based Assay for Enhanced Detection of Hematodinium sp. in the Blue Crab (Callinectes sapidus). Submitted to Diseases of Aquatic Organisms.

Grantsmanship: A total of $7,909,478 (Tables 6a,b) was collectively awarded to the LMRCSC partner institutions during the current reporting period which directly or indirectly impacted Center activities. Among the stakeholders that provided the awards included the US Department of Agriculture through the Northeast Aquaculture Center and the Cooperative Research Extension and Education Service, US Department of Education, US Department of Commerce through the National Marine Fisheries Service, and the Departments of Natural Resources in each partnering institution’s home state. Of the total amount of funds awarded to LMRCSC, $4,603,424 came from NOAA, whereas $3,306,054 was obtained from other agencies.

The funds provided by these agencies impacted LMRCSC research through support of its faculty and students and by development/enhancement of infrastructure. The Center will continue these ties by supporting travel to these organizations for training and education and bringing in experts in all fields of marine and fisheries research to provide seminars and workshops. Seventeen (17) proposals with a total amount of $13,639,060 (Table 7) were submitted to various funding agencies during this reporting period. Table 6a. Current leveraged funding from NOAA to LMRCSC institutions

Author Funding Agency Title of Project Start/End Date Amount

Eric Schott (COMB) NOAA-COL NOAA-COL partnership on advanced molecular technologies to conduct nucleotide based research on marine pathogens of interest in Chesapeake Bay

06/01/06-05/31/07

$50,000

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A.K. Johnson (UMES) NOAA-Monkfish Set-Aside Program

Biology of large Monkfish 5/1/06 – 4/30/07 $79,357

A.K. Johnson (UMES) NOAA-Monkfish Set-Aside Program Biology of Large Monkfish

5/1/07 – 4/30/08 $101,306

David J. Die (RSMAS) NOAA NMFS Simulation of Management Strategies 7/01-6/09 $429,732

David J. Die (RSMAS) NOAA NMFS Center for independent experts 12/02-12/ 07

$3,311,000

David J. Die (RSMAS) NOAA NMFS Habitat of shallow water groupers 7/01-6/09

$ 135,071 Dionne Hoskins (SSU) Alaska Fisheries Science

Center SSU Coast Camp for Youth June, 2007 $4,000

Malik Watkins (SSU), Dionne Hoskins (SSU)

NOAA EPP Program Habitat Restoration and Land Use Monitoring Using GIS Technology: A Student Training Program

10/1/05-9/30/10 $492,958

TOTAL $4,603,424

Table 6b. Current leveraged funding to LMRCSC institutions from Agencies other than NOAA

Author Funding Agency Title of Project Start/End Date Amount

Eric May (UMES) MD DNR Recreational angler survey in Maryland’s coastal Bays

8/1/07-7/31/08 $34,500

Dewayne Fox (DSU)

Delaware Sea Grant Residency patterns, migratory pathways and habitat use of adult Atlantic horseshoe (Limulus polyphemus) crab in Delaware Bay as determined by passive acoustic telemetry

$16,000

Joseph Okoh, Activity Coordinator (UMES)

U.S. Dept. of Education Strengthening the Marine-Estuarine-Environmental Sciences Doctoral Program

$894,000

Joseph Love (UMES), Arthur Allen (UMES), Eric May (UMES)

USDA Meeting the standards of higher education in fisheries management at the University of Maryland Eastern Shore, an 1890 land-grant institution

8/05 – 8/07

$194,550

A.K. Johnson (UMES) US EPA

ESREP (Eastern Shore Restoration and Education Program)

5/1/07 – 4/30/08 $50,000

David J. Die (RSMAS)

Gulf States Marine Fisheries Commission

An Atlantic-wide study of age and growth of Atlantic marlins

1/05 – 6/07 $128,087

David J Die (RSMAS) STANFORD FNDT

Fisheries and Living Marine Resources of Antigua

11/06-9/07 $ 59,735

David J. Die (RSMAS)

Hoover Foundation, The Explorer Club South Florida Student Shark Program

10/05-12/07 $84,000

David J. Die (RSMAS)

RAS/SFG Characterization of Fisheries and Living Marine Resources of the ABCMEM

11/06-5/07 $ 39,909

Dwight Ebanks* (RSMAS)

Royal Caribbean Fellowship Ecology and Habitat of Rays in the Southern US

8/07 – 7/08 $43,000

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Ayeisha Brinson* (RSMAS)

YCMBT scholarship Management indicators for Atlantic Billfish

8/07 – 7/08 $1,000

Ayeisha Brinson* (RSMAS)

McKnight Fellowship Management indicators for Atlantic Billfish

6/03-5/08 $50,000

Marc Frischer, Dionne Hoskins, Carla Curran (SSU)

GADNR Microbial Indexing of Saltmarsh Health and Recovery: Development of a Health/Productivity Scale

10/1/04-9/30/07 $288,077

May, E.B. (UMES) USDA CSREES Geospatial information technology use in water quality management internships for minorities and other audiences

8/01/06 – 7/31/07 $199,000

May, E.B. (UMES) Maryland Department of Natural Resources

An evaluation of the recreational fisheries in the Maryland Coastal Bays

05/01/04 – 06/30/07 $55,000

DeViney, S.; May, E.B. (UMES)

Maryland Department of Natural Resources

Historical and cultural aspects of minority participation in environmental issues

10/01/06 – 06/30/07 $37,457

Gulnihal Ozbay (DSU)

NSF EPSCOR Seed Grant Use of the eastern freshwater mussel (Elliptio complanata) in pond water management

3/1/06-9/30/07 $25,000

Gulnihal Ozbay (DSU)

NSF EPSCOR Seed Grant Nitrate reductase activity in mixed harmful algal populations from Delaware’s inland bays

9/1/06-8/31/07 $46,767

Gulnihal Ozbay (DSU)

USDA/CSREES Evans Allen funds

Oyster culture for community-based habitat restoration, water preservation and environmental conservation

11/1/06-10/31/09 $298,131

Gulnihal Ozbay (DSU)

NSF EPSCOR Seed Grant Ecological monitoring of bacterial loads in Eastern oysters (Crassostrea virginica) along Indian River Bay.

3/1/07-2/28/08 $34,497

Gulnihal Ozbay (DSU)

USGS Water Research Grant Viability of a freshwater mussel (Elliptio complanata) as a biological filter for aquaculture pond water quality.

6/1/07-2/28/07 $3,500

Gulnihal Ozbay (DSU)

Army Corps of Engineers Delaware Bay oyster restoration project and oyster stock assessment

6/1/07-2/28/08 $111,635

Rosemary Jagus (COMB)

NSF-MCB Characterization of zebrafish e1F4e family members in zebrafish

6/15/07-5/31/10 $612,209

TOTAL $3,306,054

*Students Table 7. Grants submitted by LMRCSC during this reporting period (March 1 - August 31, 2007) PI Funding Agency Title of Project Amount

Chigbu, P. (UMES); Parveen, S.; Hashem, F.; Allen, A.; May, E.

USDA CSRESS Pathogens in poultry litter applied on farmlands: occurrence,fate and transport in soils and subsurface waters in theDelmarva Peninsula.

$319,223 Pending

Chigbu, P. (UMES); May, E.; Jagus, R. (COMB); Love, J.; Johnson, A.; Ishaque, A.; Pitula, J.; Madhumi, M.; Aighewi, T.; Parveen, S. (UMES)

NSF URM Proposal: A program for recruiting, mentoring andpreparing undergraduate students for graduate studies inbiological sciences.

$716,400 Pending

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Curran, C. (SSU) Georgia Department of Natural Resources Coastal Incentive Grant

Designing K-12 Activities on Topics of Coastal Concern

$ 417,764 Pending

Curran, C. (SSU) NSF MultiStress 07: Disease and Parasite Occurrence as Indicators and Consequences of Stressed Coastal Ecosystems

$ 4,994,048 Pending

Dewayne Fox, et al. (DSU)

NSF GK-12: Promoting Research and Inquiry in Science and Mathematics (PRISM)

$2,999,914

Love, J.W. (UMES); Chigbu, P.; May, E.B. (UMES)

NSF Evaluating connectivity between coastal estuaries of Marylandand the Atlantic Ocean

$448,275 Pending

Love, J.W. (UMES) NOAA Importance of transient and diadromous fish species on scale-dependent trophic interactions within the Blackwater Riverdrainage (Maryland, U.S.A.)

$167,709 Pending

Ozbay, G. (DSU); Chigbu, P. (UMES); Ewart, J.; Parveen, S.

USDA Surveys of microbial, chemical and physical parametersaffecting seawater and shellfish at restoration areas inDelaware and Maryland

$499,519 Pending

David J Die NTVI

Integrated Ocean Observing System Products for IntegratedEcosystem Assessments

$ 93,486

Barbara Abraham George Burbanck (HU) NSF

GK – 12 DGE-0742346 $2,500,000

Dewayne Fox (DSU) USDA-NESARE Expansion of the Small Farm Water Quality Initiative

$175,000

Dennis McIntosh (DSU)

USDA- NRAC Innovative Techniques for Freshwater Prawn CultureMachrobrachium rosenbergii Reusing Old Screens from HardClam Mercenaria mercanaria Culture and Regional Feasibilityof Macrobrachium rosenbergii Culture

$72,005 (Not Funded)

Dennis McIntosh (DSU)

USDA-Higher EducationChallenge Grants

Collaborative Development of Model UndergraduateAquaculture Courses - Part 1. Introduction to Aquaculture

$500,000 (Not Funded)

Gulnihal Ozbay (DSU)

National Fish and WildlifeFoundation, DelawareEstuary Watershed GrantsProgram

Land use effects on Delaware’s freshwater mussels:Implications for habitat restoration and conservation

$46,827

Gulnihal Ozbay (DSU)

USDA-CSREES Viability of a freshwater mussel (Elliptio complanata) as abiological filter

$10,000

Gulnihal Ozbay (DSU)

NOAA Oceans andHuman Health Initiative

Controlling uptake and persistence of pathogenic viruses inshellfish.

$175,000

Andrea Johnson (UMES) Anne Richards (NOAA)

NOAA NMFS MonkfishSet Aside Program

Influence of climate on the distribution and catch rates ofmonkfish, Lophius americanus

$75,895

Total $13,539,060

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Objective 2: Fully Integrate Research Programs with NOAA Fisheries to Ensure Long Term Funding and Programmatic Stability The LMRCSC through the Director and DRS has been developing a proposal that will link all of the participating institutions and the NMFS (NEFSC and SEFSC) in a project that would investigate black sea bass (population dynamics and ecology). The project would directly address NMFS priorities for black sea bass research which include determination of stock size, indices for determining recruitment, and key biological reference points for the species (age to maturity, length at age, age and length at sex transition, etc.). The project will determine if latitudinal differences exist with regard to key biological reference points, and compare the differences in habitat requirements and migrations between the Mid- and South Atlantic Bights. LMRCSC has been working closely with NOAA NMFS scientists in developing the concept and a proposal will be submitted through the NOAA “Broad Agency Announcement Request for Extramural Research, Innovative Projects, and Sponsorships”. It is anticipated that through close cooperation with NOAA scientists a highly competitive project will be produced. SECTION II – EDUCATION AND OUTREACH EFFORTS LMRCSC Education and Outreach efforts are addressed through the following goal and objectives: Goal 3: Generate a Pool of Scholars Entering the Field Of Marine Science and Fisheries The objectives which accomplish this goal include: Objective 1: Enhance Recruitment of Students From Underrepresented Groups into Marine Science Disciplines Objective 2: Strengthen Outreach & Education Programs to students and the public Objective 3: Develop Programs that Enhance the Practical Education of Undergraduate and Graduate students in the Marine Sciences Objective 1: Enhance Recruitment of Students From Underrepresented Groups into Marine Science Disciplines Total student support for this 6 month reporting period was $386,379.65 or 17% of $2,299,996 received by LMRCSC during Year 1 of the award period. Sixty five (65) bachelors, 24 masters, and 5 doctoral students were supported during the current reporting period through fellowships, scholarships, stipends and travel awards. Students who received support during the current reporting period are shown in Appendix III.

Recruitment into Marine Science Programs A comprehensive list of each student receiving direct support during this reporting period is shown in Table 9. Recruitment activities by LMRCSC partners are multi-pronged and are increasingly successful, particularly in the caliber and level of motivation of incoming students. Recruitment has been facilitated through increased outreach of LMRCSC using the following modalities: 1) LMRCSC web site, 2) LMRCSC newsletters and brochures, 3) Participation in AFS meetings which provide wide exposure, and 4) Personal contact. The following efforts were made to promote LMRCSC and foster recruitment of the best possible students:

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• HBCU Research Day, held at UMES on April 12, 2007 presented an opportunity to recruit students from the mid-Atlantic region and included students from several LMRCSC partners as well as other institutions. Virginia State University graduate Rosalind Booker was recruited to the LMRCSC Masters program at UMES and Hampton University student Coley Speaks showed strong interest in UMES following this event.

• LMRCSC Director Paulinus Chigbu and Program Manager Todd Christenson visited NOAA EPP on July 10, 2007 to present opportunities in LMRCSC to the NOAA Undergraduate Scholars.

• Program Manager Todd Christenson attended the presentation session held at NOAA EPP on August 3, 2007 to further discuss LMRCSC with the Undergraduate Scholars.

• Faculty from UMES visited the Virginia Institute of Marine Science laboratory at Wachapreague, VA to discuss potential graduate projects with Hampton University student Coley Speaks.

• LMRCSC Director Paulinus Chigbu and Program Manager Todd Christenson visited the Center of Marine Biotechnology on July 24.

• Several students from the University of Arkansas Pine Bluff, University of Alaska Southeast, and the University of Delaware were introduced to LMRCSC and its open ocean research efforts during a cruise aboard the R/V Albatross IV from August 20 – 29, 2007. There are, among these students, several potential recruits for LMRCSC Masters and PhD programs.

• A new Ph.D student, Kate Gillespie has been recruited at COMB. Kate will work with Dr. Kevin Sowers on a project to examine microbially driven detoxification of PCBs in marine sediments.

• During this period no recruitment activities were conducted at RSMAS because recruitment for students that enter the fall graduate program is done mainly between September to February. Students recruited during this reporting period are shown in Table 8 below.

Table 8. Students recruited into the LMRCSC program during the current reporting period.

Last Name First Name Institution Degree Grant Ar'Quette DSU B.S. Hampton Jennifer DSU B.S. Hughes Jenaire DSU B.S. Moore Mirinda HU B.S. Moore Johnny SSU B.S. Bain Sigourney SSU B.S. Benton Azzell SSU B.S. Boyd Shaquita SSU B.S. Brooks Sheree SSU B.S. Crumley Jacelyn SSU B.S. Ezeh Linda SSU B.S. Hogfeldt Amber SSU B.S. Kinney Jennifer SSU B.S. Lawrence Monee SSU B.S. Martin Jessica SSU B.S. Moore Johnny SSU B.S. Wagner Mark SSU B.S. Aultman Terry SSU M.S. Dumont Devin SSU M.S. Kreuger Shelly SSU M.S. Marshall Joe SSU M.S.

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Pressler April SSU M.S. Williamson Christopher SSU M.S. Hicks Jeremiah UMES B.S.

Booker Rosalind UMES M.S. James Jacklyn UMES M.S.

Students supported at the Center during this reporting period are presented by degree in Appendix III, whereas students who graduated during this reporting period are presented in Table 9. Additionally, students who worked in the labs of LMRCSC scientists are presented in Table 10. Table 9. Number of students who graduated during this reporting period (March 1-August 31, 2007)

Last Name First Name Academic Institution Degree

Singleton Megan SSU M.S. Anuszewski Erica UMES B.S. Babineaux Cara HU B.S. Bain Sigourney SSU B.S. Boyd Shaquita SSU B.S. Brackerty Apprielle HU B.S. Carpenter Ashley SSU B.S. Curtis Kamau HU B.S. Davis Vernisha SSU B.S. Folawiyo Oluwasina COMB/UMES B.S. Henderson Ebony SSU B.S. Henderson Nicole HU B.S. Jones Matthew DSU B.S. Kinney Jennifer SSU B.S. Lawrence Monee SSU B.S. Leonard Justin HU B.S. Mace Trey SSU B.S. Manley Justin SSU B.S. Modeste Tracy SSU M.S. Nance Ashley HU B.S. Scoville Nicholas SSU B.S. Seaforth Liane UMES B.S. Stanley Amber SSU B.S. Thomas LaTerica SSU B.S.

Table 10. Students who worked in laboratories of LMRCSC scientists who served as their mentors and have received stipend support during this performance period.

Last Name First Name Academic Institution Degree Year

Heggins Deondra UMES B.S. Junior Hicks Jeremiah UMES B.S. Senior McWilliams Nathan UMES B.S. Junior Miller Thomas UMES B.S. Senior Nguluwe Belita UMES B.S. Sophomore

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Rice Jhamyllia UMES B.S. Senior Bain Sigourney SSU B.S. Senior Benton Azzell SSU B.S. Senior Boyd Shaquita SSU B.S. Graduated Brooks Sheree SSU B.S. Senior Carpenter Ashley SSU B.S. Graduated Crumley Jacelyn SSU B.S. Senior Dixon Tamika SSU B.S. N/A Ezeh Linda SSU B.S. Senior Henderson Ebony SSU B.S. Graduated Hogfeldt Amber SSU B.S. N/A Jackson Qadree SSU B.S. Senior Kinney Jennifer SSU B.S. Graduated Lawrence Monee SSU B.S. Graduated Love Lakeisha SSU B.S. Senior Mace Trey SSU B.S. Graduated Marchant Garth SSU B.S. Freshman Martin Jessica SSU B.S. Junior Miller Katie SSU B.S. N/A Modeste Tracy SSU B.S. Graduated Moore Johnny SSU B.S. Senior Okolo Jahlion SSU B.S. Junior Robinson Sierra SSU B.S. N/A Stanley Amber SSU B.S. Graduated Thomas LaTerica SSU B.S. Graduated Wagner Mark SSU B.S. Junior Washington Danielle Miami B.S. Senior Davis Jeanette HU B.S. Senior Lewis Alexander HU B.S. Junior McGeachy Courtney HU B.S. Sophomore Moore Mirinda HU B.S. Senior Smith Ashley HU B.S. Senior Wilkerson Carissa HU B.S. Sophomore Wilson Hamani HU B.S. Sophomore Brady Marissa DSU B.S. Senior Grant Ar'Quette DSU B.S. Junior Hampton Jennifer DSU B.S. Junior Hughes Jenaire DSU B.S. Junior Jones Matthew DSU B.S. Graduated Flowers Brandon COMB/UMES B.S. Senior Morvan Danielle COMB/UMES B.S. Sophomore Okhuereigbe Agnes COMB/UMES B.S. Junior Shelton David COMB/UMES B.S. Junior Shelton Daniel COMB/UMES B.S. Sophomore Thompson Kedena COMB/UMES B.S. Senior Townsend Abram COMB/UMES B.S. Junior Williams Kimberley COMB/UMES B.S. Senior Stiell Brian COMB/HU B.S. Senior

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Table 11. Students who participated in internships at NOAA or other agencies. Last Name First Name Year Major Institution Internship Site

Brooks Sheree Senior Chemistry SSU COMB Henderson Ebony senior Marine Science SSU Galveston lab

Hanif Ammar Senior Biology Morgan State COMB

Stiell Brian Senior Marine Science HU COMB

Smith Ashley Junior Marine Science HU National Aquarium in Baltimore

Davis Jeanette Senior Marine Science HU COMB Flowers Brandon Senior Biology UMES COMB

Folawiyo Oluwasina Grad Biochemistry Rider University COMB

Nguluwe Belita Junior Marine Science UMES COMB Morvan Danielle Sophomore Agriculture UMES COMB

Okhuereigbe Agnes Biology UMES COMB Shelton Daniel Sophomore Biology UMES COMB Shelton David Junior Biology UMES COMB

Thompson Kedena Junior Agriculture UMES COMB Townsend Abram Junior Marine Science UMES COMB Williams Kimberly Senior Biology UMES COMB

Objective 2: Strengthen Outreach & Education Programs to students and the public Accomplishments – In this reporting period, LMRCSC faculty worked on a wide range of projects, strengthening outreach and education programs to students and the public. This, in turn, resulted in additional grant awards (Table 6), proposals that have been submitted to various agencies for funding (Table 7) and collaborative projects, which are summarized below. • Upward Bound Marine and Estuarine Science Program at UMES: Dr. Andrea Johnson joined

forces in 2006 with Dave Blazer, Director of the Maryland Coastal Bays Program, to create a marine science component to the existing Upward Bound program for high school students at UMES. This program offers lessons in marine science and coastal ecology to twenty – twenty five students in grades 10-12 from Somerset, Worcester and Wicomico Counties, MD. The program draws on the expertise of UMES-LMRCSC faculty and staff as well as MD Coastal Bays Program, National Park Service and MD Department of Natural Resources, and takes place at the UMES Coastal Ecology Teaching and Research Laboratory both during the summer (six sessions) and on selected Saturdays (8 sessions) during the academic year. The program concluded its second year during the summer of 2007.

Though LMRCSC faculty, staff and students are involved in the planning and execution of all sessions, two exclusively featured LMRCSC research. On April 14, Dr. Joseph Love led a session on fish

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diversity and ecology and, on April 28, Drs. Andrea Johnson and Paulinus Chigbu taught monkfish biology and zooplankton ecology, respectively. Fourteen LMRCSC students volunteered to mentor students and assist with the execution of these sessions.

The six summer sessions (June 20, June 27, July 5, July 11, July 18 and July 25) were designed to introduce students to the often challenging process of fisheries legislation. The group of 25 local high school students from the Lower Eastern Shore explored the environmental and economic implications of a bill originally introduced in the Maryland state senate which proposed extending the zone in which bottom trawls are banned from 1 to 3 miles offshore. Led by LMRCSC faculty and graduate students from UMES, and assisted by professionals from the National Park Service and Maryland Department of Natural Resources, and members of the Ocean City fishing community, students played the roles of scientists and commercial and recreational fishermen while preparing for and carrying out a debate on the issue. Three students were assigned to be members of a Fisheries Management Council, who rendered a decision following the debate. An audience of approximately 100 people, including students from the entire Upward Bound program, parents, local legislators and fishermen attended the final session, giving the students experience at public speaking.

• NOAA and DC Educators Moving Ocean Science Forward (NEMO).

As one component of the NOAA-NEMO program directed by Laura Oremland and Dr. Bonnie Ponwith (NOAA Office of Science and Technology), LMRCSC developed a two day program on June 21 and 22 to introduce 28 DC area students from the NEMO program to the

various fish habitats of the Maryland Coastal Bays. Students worked alongside LMRCSC faculty and graduate students as they conducted sampling for their research. Collected fish specimens were identified and dissected in the lab the following day. Students were also taken offshore aboard a local charter fishing vessel to examine black sea bass habitat. In a follow-up survey, approximately 88% of the DC students said the trip increased their interest in marine science and approximately 44% of the DC students said the trip interested them in seeking out a marine science research experience (e.g., internship) (Oremland, pers. comm.) (Photos courtesy Dr. Bonnie Ponwith).

• Sea Education Association (SEA) President, Dr. John Bullard visited UMES on February 23, 2007 to

discuss the SEA program. As a direct result of this visit, LMRCSC undergraduate Danielle Morvan is participating in the program in Woods Hole in fall, 2007 starting August 28.

• Blackwater National Wildlife Refuge (Cambridge, MD): Dr. Joseph Love (Research Associate –

UMES) and a graduate student (Josh Newhard) are currently conducting research in collaboration with the US Fish and Wildlife Service on fish assemblages and fish passage in the Little Blackwater River. A publication is currently in preparation and will be presented at the American Fisheries Society meeting in San Francisco in September, 2007.

• American Fisheries Society Hutton Program: Dr. Joseph Love hosted a high school student, Steffi

Thomas, for 8 weeks during the summer of 2007 as part of the competitive Hutton Program (www.fisheries.org/afs/hutton.html). Steffi worked with Dr. Love and his students on research projects on the dietary habits and recruitment of coastal fishes.

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• Earth Day Cleanup: UMES Masters student Reginald Black organized a cleanup effort on campus in

honor of Earth Day on April 22, 2007. Five LMRCSC students participated in addition to approximately 15 others.

• SSU Coast Camp: The SSU component of the LMRCSC

conducted a marine science camp based on Ocean Literacy principles for youth aged 7-18 in June, 2007 and enrolled 86 students (93% African American). This camp also provided training and jobs for 6 collegiates, 4 of whom were also African American. Approximately 112 applications were received for the half day camp (7:30am-1pm). Additional funding ($4000) and material support (establishment as a free lunch site) was obtained from the Alaska Fisheries Science Center and the City of Savannah. Materials advertising the LMRCSC Coast Camp were submitted to the Savannah Morning News for inclusion in its annual camp guide and were distributed by the Savannah Chatham County Board of Education.

• South Florida Student Shark Program at RSMAS: In this period the South Florida Student Shark

Program continued to be the main outreach and high school educational activity conducted by the LMRCSC at RSMAS. The LMRCSC supports the activity by partially funding the participation of a MA graduate student in the Marine Affairs and Policy program, Anthony di Silvestro, who acts as coordinator of the field program of the SFSSP. In this period a third school (Palmer Trinity joined the program and successfully participated in the field and laboratory program. During this period an evaluation of the impact of the program on the students participating in it was initiated and data to support the evaluation was collected. Analysis of these data will be reported in the next LMRCSC report, because it was not completed until after September 2007. The South Florida student shark program describe above is the only LMRCSC activity that involves undergraduate students at the University of Miami. These students do not receive support for their participation and do it voluntarily. Twelve undergraduate students from the UM Marine Science undergraduate program participated in the SFSSP.

• Water Quality Monitoring in the Hampton River at HU. Eric Wooden is continuing to engage 11 residents along the Hampton River. Test kits including hydrometer, D.O. kit, secchi disk, thermometer and a questionnaire about other factors (e.g. weather and algal blooms) have been distributed to eleven residents. In this way, a network of sampling can be established and community interactions will be enhanced.

• Community Service: LMRCSC students are expected to be good citizens as well as good students.

To this end, in this reporting period, the following community service activities were performed by LMRCSC students, faculty and staff.

Table 12. Community Service and Outreach Activities at the LMRCSC

Organization/Activity Institution Date Living Shoreline Restoration UMES May, 5, 2007, July 13, 2007 Visit to Student Contest Winner at DeRenne Middle School SSU March 2007 Upward Bound Marine and Estuarine Science Program UMES April – July, 2007

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Earth Day Cleanup UMES April 22, 2007 Coast Camp for Youth SSU June, 2007 NOAA and DC Educators Moving Ocean Science Forward UMES June 21-22, 2007 Citizen’s Water Quality Monitoring Program HU Continuous • Newsletter: The Center currently publishes a newsletter, The Living Sea, to highlight Center research

and educational activities. The newsletter enhances the awareness of the LMRCSC locally and nationally. No new issues were published during this reporting period. All issues of this newsletter are available for download in PDF format from the Center’s web site (http://www.umes.edu/lmrcsc). Media coverage of LMRCSC accomplishments is shown in Appendix IV.

• Website: The LMRCSC web page is maintained by Center Program Manager Todd Christenson. The website incorporates updates on Center activities such as research, course materials, and seminar schedules. Since March 2005, Dr. Joseph Love of UMES has maintained a page devoted to the Center’s fish reference collections, which is available to education and research agencies and is continually updated. A new LMRCSC web page was mostly completed during this reporting period and deployment by the end of the fall semester, 2007 is anticipated.

DSU has established an Aquatic Sciences website (http://cars.desu.edu/aqua-sci) that has information regarding student opportunities and current research projects including LMRCSC supported activities. Additionally, there is an official webpage for the Department of Agriculture and Natural Resources on DSU’s website outlining the programs and course requirements (http://www.desu.edu/colleges/cars/ag_nat_res/index.php). In addition, the site has direct links to the LMRCSC main web page, and partner institution web pages.

Outreach events • Program Manager Todd Christenson staffed the LMRCSC booth at the following public events in order

to promote LMRCSC’s research and education activities: Beyond the Boardwalk, a family oriented ocean awareness event sponsored by the National Aquarium in Baltimore was held on June 29, 2007 in Ocean City, Maryland. Representatives of the National Park Service and Maryland Department of Natural Resources also participated. As a direct result of this event, Ashley Smith (Hampton University) was recruited for the August, 2007 research cruise aboard the Albatross IV. The Delmarva Blue Crab Festival, designed to promote both marine health as well as fisheries products took place in Cape Henlopen Park, Delaware. Other participants included the University of Maryland Center for Environmental Science laboratory at Horn Point, the Chesapeake Bay Foundation and Delaware State University.

The South Florida student shark program described above is the major LMRCSC activity at RSMAS that involves outreach and takes place through the year. Objective 3: Develop Programs that Enhance the Practical Education of Undergraduate and Graduate Students in the Marine Sciences In this reporting period, research associates and faculty conducted research funded by LMRCSC through the Technical Advisory Board which involved both graduate and undergraduate students as active research

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participants (see Goal 2 above). During the academic year, students participate in research projects at their home institutions (Table 10). A centerpiece of LMRCSC’s research and student training activities are the annual research cruises which take place aboard NOAA vessels. In March and August, 2007 (Table 13) students from LMRCSC participated in three separate cruises. These efforts have provided and will continue to provide opportunities for students to network with and gain experience from outstanding fisheries scientists. UMES undergraduates Nathan McWilliams and Claudia Harvey participated in a fisheries survey of the Hudson Canyon from March 6 – 12, 2007 aboard the Delaware II under the direction of Dr. Vince Guida of NOAA’s Sandy Hook lab. UMES Masters student Jacklyn James joined Dr. Guida, who serves on her graduate committee aboard the R/V Ronald H. Brown to study the Hudson Canyon habitat as part of her thesis research from August 8 – 20, 2007. Lastly, LMRCSC conducted a joint cruise with NOAA aboard the Albatross IV in parallel with the new fisheries vessel, the R/V Bigelow. Participants on the cruises are shown in Table 13 below. Table 13. Students who participated in research cruises aboard NOAA vessels (March 1, 2007 to August 31, 2007)

Last Name First Name Institution Vessel Date Harvey Claudia UMES Delaware II Mar 6 - 12, 2007

McWilliams Nathan UMES Delaware II Mar 6 - 12, 2007 James Jacklyn UMES Ronald H. Brown August 8 – 20, 2007 Booker Rosalind UMES Albatross IV August 20 - 29, 2007

Rice Jhamyllia UMES Albatross IV August 20 - 29, 2007 McWilliams Nathan UMES Albatross IV August 20 - 29, 2007

Smith Ashley Hampton Albatross IV August 20 - 29, 2007 Berner Logan UAS Albatross IV August 20 - 29, 2007 Ponce Micaela UAS Albatross IV August 20 - 29, 2007 Cowart Dominique UDelaware Albatross IV August 20 - 29, 2007 Morgan Edward UAPB Albatross IV August 20 - 29, 2007 Mallett Jhirmekyo UAPB Albatross IV August 20 - 29, 2007

Warfield Brandon UAPB Albatross IV August 20 - 29, 2007 SECTION III – SUCCESS STORIES (reporting period September 1, 2006 to March 31, 2007) 1. What specific contributions have the projects made to the Center, NOAA and partners? • The LMRCSC educational, research and outreach activities have resulted in several contributions

during this reporting period. Ninety four (94) students have been supported and trained in NOAA core sciences. Twenty one (21) students graduated, including 1 MS and 20 BS. A total of 11 projects are ongoing at the Center funded through the TAB review process in December, 2006. $7,909,478 in external funding is supporting Center-related activities, and 16 grant proposals with a total amount of $11,139,060 were submitted to various funding agencies. The educational and research infrastructure at the Center have been enhanced.

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• UMES’ Coastal Ecology Teaching and Research Center (CETRC): During the reporting period the lab, located at Assateague Island, has been the base of operations for several LMRCSC research projects 1) Ingression of larval fish to the coastal bays of Maryland, 2) Dietary habits of coastal fish species and 3) Habitat preference of recruiting coastal fishes. It also houses the LMRCSC fish reference collection. The facility was also used during this reporting period as a base of operations for the Upward Bound Marine and Estuarine Science Program, (April 14, April 28, May 12, May 19, June 20, June 27, July 5, July 11, July 18, and July 25) as well as monthly meetings of UBMSP partners). It was also used for the LMRCSC’s portion of the NEMO program for DC high school students held on June 21 and 22.

2. How many students participated in Center projects or activities? About 53 students participated in the Center projects. The names of some of the students, their research projects and presentations they made during this reporting period are presented in the section on “scholarly productivity” on pages 32 through 37. More than 140 K-12 students participated in the center educational and outreach activities. 3. What specific benefits were accrued to students, faculty members and the institution(s) by participating in the program? Students benefited from their participation in the projects through hands-on research experience, completion of theses in partial fulfillment of their degree requirements, stipend, and travel awards to conferences. Besides, some students secured employment after completion of their degree programs, or received scholarships for graduate studies. Some of these student awards and benefits are mentioned below: Students who received Training at NOAA Laboratories or at Center Institutions a) Twelve students (Table 13) were trained in open ocean fisheries research aboard three NOAA research vessels. b) UMES Masters student Daniel Cullen received training in age and growth techniques at the Northeast Fisheries Science Center in Woods Hole in March, 2007 and July, 2007. Dan’s research, the biology of large monkfish, is supervised by Dr. Andrea Johnson (UMES). c) Larry Alade (UMES) is working on yellowtail flounder tagging, growth and mortality at NOAA Woods Hole Laboratory under the supervision of Steve Cadrin. d) Mr. Dwight Ebanks (RSMAS) received a scholarship from Royal Caribbean to support one semester of his PhD at RSMAS. Ms. Ayeisha Brinson (RSMAS) continued to receive support during this period from the McKnight foundation to partially support her research work and degree. 4. To what extent has the projects or activities enhanced and improved outreach, education, training and NOAA related research at the institutions? a) LMRCSC students at all institutions play an active role in community outreach (Table 12), partly due to the higher standard to which the Center holds its students. b) Two UMES graduate students, Lonnie Gonsalves and William Gardner have created the American Fisheries Society student subunit at UMES which boasts more than 20 members. c) The LMRCSC has enhanced ocean science literacy among K-12 students and the public through the Upward Bound Marine and Estuarine Science Program, SSU’s Coast Camp, and other programs.

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5. Did students participate in site visits to NOAA laboratories and/or facilities? a) Fifteen (15) students were trained at NOAA labs or on NOAA sponsored research cruises during this reporting period. (Table 13 and Section III.3 above).

SECTION IV – REVISIONS TO TASKS AS DESCRIBED IN GRANT AWARD AMENDMENTS AND THE IMPACT TO THE AWARD There were no amendments to the award.

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Appendix I. Summary of Responses by LMRCSC Students at UMES to LMRCSC Exit Evaluation Questions Part I. 1. The LMRCSC program basically fulfilled my expectations

Agree Strongly Agree Agree Strongly Agree Undecided Agree Agree Agree Agree

2. After completing the LMRCSC program, I am more likely to consider marine science/fisheries as a possible career choice

Agree Strongly Agree Strongly Agree Strongly Agree Agree Agree Strongly Agree Disagree Undecided

3. I believe that my professional opportunities will be greatly increased by my participation in the LMRCSC programs

Agree Agree Strongly Agree Strongly Agree Agree Strongly Agree Agree Strongly Agree Strongly Agree

4. My participation in the LMRCSC has been a positive experience

Strongly Agree Agree Strongly Agree Strongly Agree Undecided Agree Agree

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Agree Strongly Agree

5. I would recommend the LMRCSC program to other students

Agree Agree Strongly Agree Strongly Agree Agree Strongly Agree Undecided Strongly Agree Strongly Agree

Part II 1. In what ways did your participation in the LMRCSC program assist you with reaching your academic potential?

Equipped me with the necessary tools to achieve excellence in the field of natural resources/marine science

Aided tremendously with various research opportunities that enhanced awareness in the field and academically

Program was a wonderful networking base Provided me with experience and opportunities to adequately compete with my peers for

fellowships, assistantships, and acceptance into graduate programs Provided me with countless research experience opportunities; I was engaged intellectually

through conversations with LMRCSC staff; Hosted an array of seminars and sponsored trips to scientific conferences which were intellectually enriching

Allowed me to attend various conferences, improving my presentation skills, and allowing me to network with scientists from various agencies, organizations and universities; Performed research at a NOAA fisheries lab.; I will now be a contract technician at this lab.

Provided me with the know with which to take care of the environment Afforded me the opportunity to conduct my research at another facility and provided me

financial support. Tutelage of several teachers has proved critical to my academic success Provided great advising and opportunities Learned valuable laboratory skills; gave me the opportunity to meet wise and respectable

scientists who have inspired me. 2. What are your future career plans and do you feel that your participation in the LMRCSC

program helped you attain them? To obtain a Ph.D. or work in the field of related sciences; my participation in the LMRCSC has

helped prepare me for many broad areas in the this field To earn a Ph.D. in fisheries ecology; LMRCSC helped me to focus my interests To obtain a Ph.D. in marine science from College of William and Mary, VA Eventually obtain a Ph.D. in ecology, zoology or marine toxicology; Will work as a research

technician in a fisheries lab for now; thanks to LMRCSC for helping me achieve my goals

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Certain classes prepared me for Environmental Policy issues which I am interested in. May be work for NOAA or some other government agency. Working with plants, including aquatic species M.S. degree in Social Work at the Catholic University of America Ph.D. in microbiology, genetics or cancer field.

3. If you plan to go on to graduate school, where do you want to go and what would you want to study? => Ph.D. degree but I’m undecided about what to study => Ph.D. degree at College of William and Mary, VA => VIMS; predator-prey interactions of benthic and epibenthic species => Ph.D. in marine-related field; not sure which university => UMES MEES program; project related to ecosystem management => Ph.D. at UMES => May be Ph.D. in the University System of Maryland => M.S. Social Work, Health Care; Catholic University of America => Stanford University, Johns Hopkins, UMES; microbiology, genetics, biotechnology 4. Describe any perceived external barriers or obstacles that blocked the achievement of your full potential in the LMRCSC program => Lack of communication with targeted advisors for proper training and mastery in a given program => Lack of “set” structure with the universities system => Since fall 2004 LMRCSC has been geared towards helping students gain real world research experiences. => None => I feel UMES would have provided a poor environment for me, had the LMRCSC not been part of my graduate experience; Faculty associated with the LMRCSC have been, as a whole, steps above regular UMES faculty. => Guidance was not as strong as I needed. => The only external barriers are within the university system in which we do not have any control over; A lot of students do not have external family financial support and when paychecks are delayed this puts them in a bad situation. => No access or knowledge of available funds; lengthy delays in renewing contractual help. => Knowledge of the various areas of fisheries => Lack of courses in microbiology and biochemistry at the upper level 5. Describe any perceived internal barriers (i.e., within yourself, or personal) difficulties that blocked the achievement of your full potential in the LMRCSC program => My lack of consistency throughout the duration of my graduate program => None; the staff and faculty provide students with experiences and support that instill confidence

and competence => None => I had a lack of knowledge or was weak in some areas and that prevented me from finishing the

classes I needed to stay in the dual degree program. => I need to be more aggressive. => Apathy and irritation brought about by external factors => None

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=> In the beginning, I lacked confidence 6. After completing the LMRCSC program, what personal qualities or characteristics do you feel are necessary for someone who wants to make a successful career in marine science/fisheries? => Positive/enthusiastic attitude; Ability to do both field and lab. research; Leadership abilities => Passion for chosen field and determination => Intellectually sharp, inquisitive, open; respect for the environment => Motivation, willingness to learn and work, dedication, high standards of expectations, etc. => Patience, perseverance, resilience. => Independence => Ability to lead => Determination, attention to detail, patience => Passion, patience, ability to work independently and as a member of a team 7. How can the LMRCSC program be improved?

Student union (undergraduate & graduate); more outreach to high schools (recruitment) More diverse background among staff/faculty; programs to ensure young scientists stay in the

field; more communication between associated schools A new building ---- and not a trailer for the distinguished individuals who are working hard at

improving the LMRCSC program; more recognition from the campus Raise expectations from students, i.e. 4 years of a masters degree is unacceptable Provide guidance to students as soon as they enroll in the program to avoid mistakes I don’t believe that it should be necessary to go to another place to conduct a worthy research

project. More cross-training Provide overview of the LMRCSC program as a whole for incoming students The program is wonderful.

8. Additional Comments

Overall LMRCSC is a wonderful program; outreach should be focused more on surrounding high schools to promote the program among those unfamiliar with marine science/fisheries

I truly had a great time being a part of the LMRCSC crew. LMRCSC is what kept me at UMES for the duration of my graduate education. This is a great

program with some improvements needed; Allows students to network and present in a public forum is critical to the student experience, and a great quality of the program.

More needs to be done to aid students in the MEES entry process. Program offered me many opportunities and a broader view of marine sciences and NOAA.

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Appendix II: Other Projects in Support of Undergraduate or Graduate Involvement in Research The following are the complete descriptions of projects not vetted through the TAB, but rather conducted at each institution in support of undergraduate or graduate student projects. FISHERIES SOCIOECONOMICS 1. Project Title: A survey of recreational angling in the Maryland Coastal Bays Project Description: The Maryland Coastal Bays represents a diverse system of small bays, rivers and streams separated from the Atlantic Ocean by a barrier island (Assateague). The Maryland Department of Natural Resources (MDNR), in order to better allocate its resources, developed a collaborative study with UMES to determine the level of angling effort, mode of angling, spatial distribution of angling as well as gathering demographic and economic information on individual anglers. The study has now been conducted over the past four summers (2004 through 2007) between the last week of May and the first week of September with anglers interviewed using a survey form developed in cooperation with NOAA Marine Recreational Fisheries Statistical Survey (Maryland Coordinator), MDNR and UMES. The 2006 study was designed to determine the total angling effort in the coastal bays using a combination of boat, aerial and roving surveys coupled with individual angler interviews which was different that the 2004 and 2005 surveys where boat and aerial surveys were not employed. For 2007, the study was expanded to include off-shore recreational fisheries assessments. Thematic Area Addressed: Socioeconomics Lead Scientist(s): Dr. Eric B. May (UMES) NOAA Collaborator(s) (please include affiliation): None LMRCSC Collaborator(s): Dr. Joseph Love (UMES) Collaborator(s): Mr. Martin Gary (Maryland Department of Natural Resources) and Mr. Mike Luisi (Maryland Department of Natural Resources) LMRCSC Research Student(s): Ms. Erica Anuszewski (UMES BS/MS Student) Planned Start Date: May 2004 Planned End Date: September 2008 Planned and actual results of project: Planned – Based on the results of three years of angler interviews the project will be redirected towards an assessment of the general tourist population to determine the significance of “bay based” recreational angling to tourism. This effort will include economic and demographic interview questions. Actual – Four surveys during the summers (last week of May through first week of September) of 2004, 2005, 2006 and 2007 were conducted. The 2004 survey was a preliminary ground-truthing survey to determine spatial and temporal extent of future surveys. Since the 2004 survey was based on the NOAA Marine Recreational Fisheries Statistical Survey (MRFSS) the preliminary survey provided a refined survey instrument for subsequent surveys based on angler responses in 2004. In 2005 the survey was expanded to include 32 shore based sites in the coastal bays encompassing all of the bays and tributaries except the

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St. Martins River and Chincoteague Bay. This expansion was the result of the 2004 spatial survey which identified where approximately 95% of the share based recreational angling was taking place in the coastal bays area. In addition, at the request of MDNR the 2006 survey incorporated questions related to receptiveness towards a marine fishing license. In 2006 in collaboration with MDNR the study was further expanded to include all recreational shore and boat based angling in the coastal bays (including the St. Martins River and Chincoteague Bay) as well as surf fishing on the Atlantic Coast side of Ocean City and Assateague Island. This included boat and aerial surveys of recreational angling from boats. All 2006 data has been entered and data quality assurance checks performed. Preliminary analyses of the data suggest the following: 1) overall angling activity decreased in 2006, 2) boat based angling was more significant that previously thought, 3) surf fishing is a minor component of the recreational angling, 4) surf fishing off Assateague Island is a highly homogeneous economically and demographically, and 5) the overall contribution of recreational angling to the tourism of Ocean City is small. The 2007 survey has just been completed for the year, with only preliminary results available. However, based on initial examination of the data, tourists in general do not come to Ocean City for angling. Approximately 5% indicated that angling was the primary reason for their trip and over 60% would not fish during their stay. How will results be incorporated into NOAA Fisheries operations? The proposed research is consistent with NOAA’s goals and the LMRCSC’s Targeted Research Theme of Socioeconomics. The data will provide to MDNR and the Mid-Atlantic Council accurate information of catch and effort for several key recreationally important fish species, and the significance of bay based recreational angling in Maryland. For Worchester County, the data will provide a better assessment of the importance of bay based recreational angling to the economy of the County. How will results be incorporated into LMRCSC research and curriculum? Under the new cooperative agreement the LMRCSC was charged with emphasizing socioeconomics and an important aspect of its research. In particular, the issue of community vulnerability was considered one of the key issues. This project is examining the importance of recreational angling to the community of Ocean City and the potential impact that would occur if a license was imposed for the area. The 2007 survey provided training for 5 interns and will serve as the basis for a master’s thesis.

2. Project Title: Historical, Cultural and Environmental Assessment of African Americans in the Lower Eastern Shore Maryland Project Description: The Lower Eastern Shore Maryland encompasses the counties of Wicomico, Worcester, and Somerset, where African Americans have a significant connection to the land and aquatic resources as farmers, watermen, seafood processors and consumers. Recent efforts on the part of the Maryland Department of Natural Resources (MDNR) and the Maryland Coastal Bays Program (MCBP) to reach out to this segment of the community have largely met with failure. The identified reasons are: inability to define community leaders, inability to understand communication centers, lack of understanding of community issues related to the environment and use of language that failed to reach the community. To rectify this situation MDNR has engaged UMES through the LMRCSC to conduct a study of the African American communities of the Lower Eastern Shore to determine nodes of communications, issues important to the community, environmental issues important to the community and the mode of communication most suited to reaching the community. As education an outreach programs are developed

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by MDNR and environmental policies (land use, fisheries policies, management plans etc.) are being formulated it is important to engage all segments of the communities affected. This study is the first step taken by MDNR and MCBP in this effort. Thematic Area Addressed: Socioeconomics Lead Scientist(s): Dr. Stanley DeViney (UMES) NOAA Collaborator(s): Collaborator(s): Ms. Sasha Bishton (Maryland Department of Natural Resources), Mr. Vincent Leggett (Maryland Department of Natural Resources), Mr. David Blazer (Maryland Coastal Bays Program), LMRCSC Research Student(s): Ms. Lianne Seaforth (UMES) Planned Start Date: October, 2006 Planned End Date: July, 2007 Planned and actual results of project: Planned – The project is divided into three phases: 1) Development of community structure maps related to demographics, economics, and access to resource; 2) Convening of focus groups representing a segments of the community; and 3) surveys of the communities to identify issues and priorities for the community as it relates to the environment. Actual – At this time all community structure maps have been developed based on US Census and county data bases. Focus groups have met and the results recorded and evaluated. Surveys have been conducted in each of the counties and results entered. A preliminary report is in preparation. How will results be incorporated into NOAA Fisheries operations? This research is consistent with NOAA’s goals and the LMRCSC’s Targeted Research Theme of Socioeconomics. The data will provide to MDNR and MCBP information on African American communities that will better engage them in environmental issues as well a provide direction for education and outreach programs. How will results be incorporated into LMRCSC research and curriculum? Under the new cooperative agreement the LMRCSC was charged with emphasizing socioeconomics and an important aspect of its research. In particular, the issue of community vulnerability was considered one of the key issues. This project examines the importance of environmental issues to one of the most vulnerable segments of the community and will provide the basis for the LMRCSC to collaborate with MDNR and MCBP in outreach and educational programs for K-12 and the community.

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Appendix III. Students who received support during the funding period (March 1-August 31, 2007)

Last Name First Name Academic Institution Degree Amount

Anuszewski Erica UMES B.S. 1,530.00 Bain Sigourney SSU B.S. $80.00 Benton Azzell SSU B.S. $1,760.00 Boyd Shaquita SSU B.S. $1,869.25 Brady Marissa DSU B.S. $500.00 Brooks Sheree SSU B.S. $298.70 Brooks Sheree COMB/SSU B.S. $7,940.67 Carpenter Ashley SSU B.S. $333.00 Crumley Jacelyn SSU B.S. $1,869.25 Davis Jeanette COMB/HU B.S. $7,037.76 Dixon Tamika SSU B.S. $60.00 Dominique Vincent UMES B.S. $2,994.00 Ezeh Linda SSU B.S. $1,869.25 Flowers Brandon COMB/UMES B.S. $6,174.67 Folawiyo Oluwasina COMB/UMES B.S. $6,174.67 Grant Ar'Quette DSU B.S. $3,426.13 Hampton Jennifer DSU B.S. $2,440.88

Hanif Ammar COMB/Morgan

State B.S. $7,330.61 Harvey Claudia UMES B.S. $81.50 Heggins Deondra UMES B.S. $497.25 Henderson Ebony SSU B.S. $60.00 Henderson Ebony SSU B.S. $80.00 Hicks Jeremiah UMES B.S. $3,000.00 Hogfeldt Amber SSU B.S. $2,258.00 Hughes Jenaire DSU B.S. $3,795.91 Jackson Qadree SSU B.S. $945.00 James Jacklyn UMES B.S. $519.08 Jones Matthew DSU B.S. $3,711.73 Kinney Jennifer SSU B.S. $2,000.00 Lawrence Monee SSU B.S. $1,869.25 Lewis Alexander HU B.S. $500.00 Love Lakeisha SSU B.S. $60.00 Mace Trey SSU B.S. $734.00 Marchant Garth SSU B.S. $3,608.74 Martin Jessica SSU B.S. $943.00 McGeachy Courtney HU B.S. $3,000.00 McWilliams Nathan UMES B.S. $82.00 Miller Katie SSU B.S. $1,000.00 Miller Thomas UMES B.S. $1,643.40 Modeste Tracy SSU B.S. $700.00 Moore Mirinda HU B.S. $500.00 Moore Johnny SSU B.S. $5,000.00 Moore Johnny SSU B.S. $5,000.00

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Morvan Danielle COMB/UMES B.S. $7,976.67 Morvan Danielle UMES B.S. $2,994.00 Nguluwe Belita COMB/UMES B.S. $7,976.67 Nguluwe Belita UMES B.S. $3,164.00 Okhuereigbe Agnes COMB/UMES B.S. $7,654.70 Okolo Jahlion SSU B.S. $60.00 Rice Jhamyllia UMES B.S. $82.00 Robinson Sierra SSU B.S. $267.30 Shelton Daniel COMB/UMES B.S. $1,200.00 Shelton David COMB/UMES B.S. $6,174.67 Smith Ashley HU B.S. $1,500.00 Stanley Amber SSU B.S. $2,000.00 Stiell Brian COMB/HU B.S. $7,037.76 Thomas LaTerica SSU B.S. $1,869.25 Thompson Kedena UMES B.S. $6,239.73 Townsend Abram COMB/UMES B.S. $7,428.67 Townsend Abram UMES B.S. $510.00 Wagner Mark SSU B.S. $7,500.00 Wilkerson Carissa HU B.S. $4,600.00 Williams Kimberley UMES B.S. $3,696.00 Wilson Hamani HU B.S. $4,600.00 Currie Alyssa SSU/Riverhill High High School $6,627.64 Best Jr. Alexus SSU High School $327.84 Campbell Shari SSU High School $327.84 Collier-Feggins Jordan SSU High School $327.84 Franklin Michael SSU High School $327.84 Muhammad Mitchell SSU High School $327.84 Olson Katherine SSU High School $50.00 Saunders Robert SSU High School $327.84 Seales Van Ellison SSU High School $327.84 DiSIlvestro Anthony Miami M.A. $7,128.00 Allen LaTrisha SSU M.S. $6,000.00 Anuszewski Erica UMES M.S. $3,994.00 Aultman Terry SSU M.S. $2,000.00 Bacoat Donald Miami M.S. $8,970.00 Black Reginald UMES M.S. $20.00 Booker Rosalind UMES M.S. $82.00 Cairns Colette DSU M.S. $5,125.48 Chambliss Shanna DSU M.S. $2,495.85 Corbin Ryan UMES M.S. $10,059.18 Cullen Daniel UMES M.S. $10,272.20 Dumont Devin SSU M.S. $6,000.00 Gonsalves Lonnie UMES M.S. $10,059.18 Kalinowsky Chris SSU M.S. $756.00 Kreuger Shelly SSU M.S. $6,000.00 Luers Daniel UMES M.S. $10,896.57 Marshall Joe SSU M.S. $80.00 Newhard Josh UMES M.S. $5,339.51

60

Partridge Michael SSU M.S. $6,000.00 Pressler April SSU M.S. $6,000.00 Serrano Xaymara Miami M.S. $11,373.00 Simpson Philip DSU M.S. $6,102.49 Singleton Megan SSU M.S. $84.76 Willet Naeem DSU M.S. $5,125.48 Williamson Christopher SSU M.S. $5,736.00 Anthony Sandra SSU Middle School $50.00

Best Amber- Brianna SSU Middle School $327.84

Blalock Daryl SSU Middle School $327.84 Brand Casey SSU Middle School $100.00 Brazzle Jr. Jonathan SSU Middle School $327.84 Brisard Amber SSU Middle School $327.84 Brown Idellonie SSU Middle School $327.84 Brown Devenna SSU Middle School $327.84 Brown Courtney SSU Middle School $327.84 Brown Rashad SSU Middle School $327.84 Brown III Jonathan SSU Middle School $327.84 Butler Anthony SSU Middle School $327.84 Clark Edmond SSU Middle School $327.84 Cousin Justin SSU Middle School $327.84 Deacon Shaniya SSU Middle School $327.84 Deacon Zakye SSU Middle School $327.84 Dozier-Muhammad Kashta SSU Middle School $327.84 Dozier-Muhammad Askia SSU Middle School $327.84 Elam Lucy SSU Middle School $327.84 Elam Simon SSU Middle School $327.84 Finklea Olesia SSU Middle School $327.84 Forman Alex SSU Middle School $327.84 Frame Stephanie SSU Middle School $50.00 Gardner William SSU Middle School $327.84 Gardner Gabrielle SSU Middle School $327.84 Gibbons Nicholas SSU Middle School $327.84 Hall Morgan SSU Middle School $327.84 Herzog Jessica SSU Middle School $327.84 Hinman Matthew SSU Middle School $6,000.00 Hunter Khalid SSU Middle School $327.84 Johansen Keely SSU Middle School $327.84 Johnson Antwon SSU Middle School $327.84 Jones Kaitlyn SSU Middle School $327.84 Jones David SSU Middle School $327.84 Jones Winter SSU Middle School $327.84 Jones Ben SSU Middle School $327.84 Martin Jaye SSU Middle School $327.84 Maynor Tevin SSU Middle School $327.84 Mayville Julie SSU Middle School $50.00

61

McFarland David SSU Middle School $327.84 McIntosh Paige SSU Middle School $50.00 Moore Sherman SSU Middle School $327.84 Muhammad Simba SSU Middle School $327.84 Muhammad Ezekiel SSU Middle School $327.84 Mulatu Avyssinia SSU Middle School $327.84 Mulatu Samera SSU Middle School $327.84 Neal Naki SSU Middle School $327.84 Noel-Finklea O'Brian SSU Middle School $327.84 O'Donnell Bonny SSU Middle School $327.84 Patterson Jermaya SSU Middle School $327.84 Paul Jr. Ishmael SSU Middle School $327.84 Powell Alexis SSU Middle School $50.00 Rawlerson Kyle SSU Middle School $327.84 Rawlerson Tyler SSU Middle School $327.84 Shepard Cythina SSU Middle School $1,869.25 Smith Carion SSU Middle School $1,869.25 Strombeck Jacob SSU Middle School $327.84 Tanner Irkeam SSU Middle School $327.84 Watkins Asia SSU Middle School $327.84 Watkins Aishah SSU Middle School $327.84 Watkins Malik SSU Middle School $327.84 Williams Raven SSU Middle School $327.84 Williams Ashley SSU Middle School $327.84 Williams Coltrane SSU Middle School $327.84 Williams Anthony SSU Middle School $327.84 Worlds Dontre' SSU Middle School $327.84 Booker Tedra UMES Ph.D. $10,290.54 Brinson Ayeisha Miami Ph.D. $3,958.00 Ebanks Dwight Miami Ph.D. $9,636.00 Gillespie Kathleen UMBI COMB Ph.D. $1,172.47 Richardson Maria UMBI COMB Ph.D. $383.56 Reyes Jose UMBI COMB Ph.D. $7,682.36

TOTAL $386,379.65

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Appendix IV. Press coverage of LMRCSC projects and initiatives Delaware WAVE – Education: “At home with the oysters. College students aim to restore the oyster

population in the inland bays” Wednesday July 25, 2007. Web-site: www.delmarvanow.com Cape GAZETTE – “Delaware Bay Oyster Restoration Project now under way” Friday June 22, 2007. Bridgeton News – “$2 million oyster planting project to begin June 15” Saturday June 16, 2007 Press of Atlantic City – “Fisherman making Delaware Bay hospitable for oysters” Friday June 20, 2007. Daily Times (Growfish) – “At home with the oysters: A few students from Delaware State University

spending their summer with the oysters” Wednesday June 25, 2007. Web-site: www.delmarvanow.com.

DO Delaware Online – “Oysters monitored 90 garden sites” Thursday August 9, 2007. Delaware State News – A Home and Garden Guide: “Cleaning pond water with native plants” Sunday, July

15, 2007. Delaware State University Aquatic Sciences Quarterly Newsletter Spring 2007- “Graduate Student Jon

McKenzie Begins Research on Freshwater Mussels; Graduate Students Mary Lambert and Patrick Erbland Complete Research”.

Edible New Jersey Newsletter: Celebrating local food of the Garden State, season by season: Number 1. “Where the River Meets the Sea. Are restoration efforts finally paying off?” Summer 2007

Star Democrat. Group studies Little Blackwater plants. June 22, 2007.