Center for Homogeneous DNA Analysis - Software April 21, 2005.
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Transcript of Center for Homogeneous DNA Analysis - Software April 21, 2005.
Second Year Advances
• Background removal
• Melting curve calculators
• Automatic clustering and classification
Background removal
• Superior to baseline method• Enables correct classification of unlabeled
probe melting curves• Makes HLA matching, repeat typing and
sequencing more accurate• Improves determination of thermodynamic
parameters, and therefore all PCR design and analysis tools
• In use by ARUP Labs
Melting curve calculators
• More informative than just Tm calculator
• Enhances design of full genotyping studies
• Improves determination of thermodynamic parameters
Automatic clustering and classification algorithms
• Used in HLA identity testing, mutation scanning, and genotyping
WWW availability
• Center for Homogeneous DNA analysis• Homepage: www.dna.utah.edu• DNAWizards beta site by remote panels• URL: DNAWizards.path.utah.edu• Small amplicon primer design: SNPWizard• Melting curve calculators: Melt1.0, Het1.0
The coming year
• Determine a complete set of Tm Parameters • superior background removal• realistic experimental conditions (dyes, salt)• mismatched duplexes (synthetic)
• Genome wide primer promiscuity prevention• Further develop user-friendly suite of programs• Commercial launch of DNAWizards.com