Cellwasher 2 Cell Washing System

Service Manual

SORVALC Cellwasher 2

CELL WASHING

SYSTEM

SORVALL® Products

Newtown, Connecticut

U.S.A.

SORVALL Centrifuges

PN 04624-5

Issued February 1994

User

Parts list Updated Apr '05

Cellwasher 2 Service SORVALL®lnstrumentS

This manual is a guide for service of the

SORVALL® Cellwasher 2 Cell Washing System

Data herein has been verified and validated and is believed adequate for the intended use

of the instrument. If the instrument or procedures are used for purposes over and above

the capabilities specified herein, confirmation of their validity and suitability should be

obtained, otherwise, DuPont does not guarantee results and assumes no obligation or

liability. This publication is not a license to operate under, nor a recommendation to

Infringe upon, any process patents.

This service manual is intended as a service aid. While the manual is kept current and

includes information regarding significant design changes, specific designs may still vary

from instrument to instrument.

This service manual is intended for use only by service personnel who have been trained

by DuPont. Due to the high electrical potential in this centrifuge, untrained individuals

must not attempt any of the procedures in this service manual.

NOTES, CAUTIONS, and WARNINGS within the text of this manual are used to emphasize

important and critical instructions:

WARNING A Warning informs the operator of a hazard or an unsafe practice that could

result in personal injury, affect the operator's health, or contaminate the

environment.

CAUTION A Caution informs the operator of an unsafe practice that could result in

damage of equipment.

NOTE A Note highlights essential information.

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characteristics of the material and the hazards associated with it. In the .

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rotor can protect you from the particles dispersed into the air. To protect

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ii

Sorvall® Instruments Cellwashcr 2 Service

Table of Contents

Paragraph Page

1-1.

1-2.

1-3.

1-4.

2-1.

2-2.

2-3.

2-4.

2-5.

2-6.

3-1.

3-2.

3-3.

4-1.

4-2.

4-3.

4-4.

4-5.

4-6.

4-7.

4-8.

4-9.

4-10.

Section I. INTRODUCTION

Intended Users .................................... 1-1

Warnings and Cautions ..............................1-1

Service Decontamination Policy. ....................... 1-1

Warranty Responsibility ............................. 1-2

Section 2.

DESCRIPTION, INSTALLATION and OPERATION

Description of the Cellwasher 2 ........................ 2-1

Cellwasher 2 Specifications ........................... 2-1

Installation Information. ............................. 2-2

Cellwasher 2 Operation .............................. 2-10

Emergency Sample Recovery .......................... 2-17

Condensed Operating Instructions ..................... 2-18

APPENDED: Condensed Operating Instructions ........... 2-19s

Section 3. MAINTENANCE

Inspection and Cleaning ............................. 3-1

Tubing Replacement ................................ 3-5

Cellwasher 2 Preventive Maintenance Procedure ........... 3-5

APPENDED: Preventive Maintenance Checklist ........... 3-9

Section 4. MECHANICAL THEORY

Controls ......................................... 4-1

Flexible Drive Mounting ............................. 4-1

Cabinet ......................................... 4-1

Air Flow ......................................... 4-1

Motor ........................................... 4-1

Safety Latch ...................................... 4-1

Saline Pump System ................................ 4-2

Flow Detector ..................................... 4-2

Flow Control Clamp ................................ 4-2

Motor Antirotation Clutch ............................ 4-2

in

Rev. 11/90

Cellwashcr 2 Service Sorvall® Instruments

Table of Contents (continued)

Paragraph Page

5-1.

5-2.

5-3.

5-4.

5-5.

6-1.

6-2.

6-3.

6-4.

8-1.

8-2.

8-3.

8-4.

8-5.

8-6.

8-7.

8-8.

8-9.

8-10.

8-11.

Section 5. ELECTRICAL THEORY

System Description ................................. 5-1

Touch Switch Panel. ................................ 5-31

Interface PC Board ................................. 5-31

Microcomputer PC Board ............................ 5-37

Electronics Module ................................. 5-50

Section 6. APPLICATIONS

Cellwasher 2 Application ............................. 6-1

Compatibility Testing ............................... 6-1

Cellwasher 2 Washing Cycle .......................... 6-2

Glossary of Blood Banking Terms ...................... 6-6

Section 7. TROUBLESHOOTING

Section 8. REPAIR and REPLACEMENT PROCEDURES

Base Plate Removal................................. 8-1

Cabinet Removal................................... 8-1

Front Panel Removal ................................ 8-2

Power Switch Replacement ........................... 8-3

Pump Replacement................................. 8-3

Pump Motor Replacement ............................ 8-3

Flow Detector Replacement........................... 8-4

Motor Replacement................................. 8-4

Interface Printed Circuit

Board Replacement................................ 8-4

Microcomputer Printed Circuit

Board Replacement................................ 8-5

Fuse Replacement.................................. 8-5

IV

Rev. 11/90

Son/all® Instruments Cellwasher 2 Service

Table of Contents (continued)

Paragraph Page

Section 9. KIT INSTRUCTIONS

9-1.

9-2.

9-3.

9-4.

9-5.

9-6.

9-7.

Figure

2-1.

2-2.

2-3.

2-4.

2-5.

3-1.

3-2.

5-1.

5-2.

5-3.

5-4.

5-5.

5-6.

Tubing Replacement Kit (PN 04632) .................... 9-2

Collector Seal Replacement Kit (PN 04353) ............... 9-9

Retainer Ring Replacement Kit (PN 12850) ............... 9-12

Lid Latch Assembly Replacement

Kit (PN 12792).................................... 9-14

Saline Shield Kit (PN 12811) .......................... 9-19

Triac-Interface Printed Circuit Board

Replacement Kit (PN 12816) ......................... 9-20

Adapter Clip Installation Kit (PN 04330) ................. 9-23

Section 10. ILLUSTRATED PARTS LIST

List of Illustrations

Page

Cellwasher 2 Tubing Diagram ......................... 2-4

Stainless Steel Rotating Bowl Installation ................ 2-2

Distributor Installation .............................. 2-9

Cellwasher 2 Controls and Indicators ................... 2-10

Location of Mechanical Override ....................... 2-18

Exploded View, Collecting Ring Assembly

(SN 8702302 and higher) ........................... 3-3

Exploded View, Collecting Ring Assembly

(SN 8702301 and lower) ............................ 3-5

System Schematic, Cellwasher 2 ....................... 5-3

Wiring Diagram, Cellwasher 2 ......................... 5-5

Microcomputer PC Board ............................ 5-7

Schematic, Microcomputer PC Board ................... 5-11

Triac-Interface PC Board (PN 04834 Revision 0,

SN 8503865 and higher) ............................ 5-16

Schematic, Triac-Interface PC Board (PN 04537

Revision 2, SN 8503865 and higher) ................... 5-17

v

Rev. 11/90

Cellwasher 2 Service SORVALL®lnstrumentS

List of Illustrations (continued)

Figure Page

5-10. Schematic, Onterface PC Board (PN 04537

Revision 1, SN 8503864 and below) ........................ .5-25

5-11. Cellwasher 2 Automatic Wash Sequence ...................... .5-30

5-12. Indicator Current Path ................................... .5-31

5-13. Schematic, Touch Switch Panel ............................ .5-33

5-14. Interface PC Board, Test Set-Up #1 .......................... .5-35

5-15. Interface PC Board, Test Set-Up #2 .......................... .5r36

5-16. Microcomputer Board Input/Output Configuration .............. .5-38

5-17. Microcomputer Scan Routine .............................. .5-39

5-18. Creating New Input/Output Formats ........................ .5-41

5-19. Digital Time Display Segments ............................. .5-42

5-20. Starting in Manual (High or Low) Mode, Logic Flow Chart ......... .5-47

5-21. Starting from Check Mode, Logic Flow Chart ................... .5-48

5-22. Starting in Auto Mode, Logic Flow Chart ...................... .5-49

7-1. Location of Decant Coil and Drive Ring ....................... .7-7

7-2. Location of Lift Plate Ring ................................. .7-7

9-1. Tubing Diagram: Parts Location ............................ .9-2

9-2. Adjustable Tubing Clamp Location .......................... .9-6

9-3. Adjustable Tubing Clamp ................................. .9-8

9-4. CW-1 Collecting Ring Assembly Exploded View ................. .9-10

9-5. Cellwasher 2 Collecting Ring Assembly Exploded View ........... .9-11

9-6. Cabinet Machine Drawing ................................. .9-17

9-7. Adhesive Location ....................................... .9-20

9-8. Installing an Adapter Clip ................................. .9-24

10-1. Cellwasher 2 Assembly .................................... 10-5

10-2. Chassis and Component Assembly ........................... 10-11

10-3. Drive Motor Assembly ..................................... 10-15

List of Tables

Table Page

2-1. Parts Location: Tubing Diagram ............................ .2-4

2-2. Cellwasher 2 Controls and Indicators ........................ .2-9

5-1. Component Identification, Microcomputer PC Board .............. 5-8

5-2. Component Identification, Triac-Interface PC Board

(PN 04834, Revision 0, SN 8503865 and higher) ............... .5-15

5-3. Triac PC Board Component Identification ..................... .5-19

5-4. Component Identification, Interface PC Board

(PN 04536, Revision 2, SN 8503864 and below) ............... .5-23

5-5. Automatic Wash Sequence ................................ .5-30

5-6. Microcomputer Scan Map ................................. .5-40

5-7. Electronics Control System ................................ .5-43

vi Rev. 4/96

SORVALL® Instruments Cellwashcr 2 Service

List of Tables (continued)

Table Page

6-1. Applications Troubleshooting Chart: False Negative Results ........ 6-3

6-2. Applications Troubleshooting Chart: False Positive Results ........ .6-4

6-3. Applications Troubleshooting Chart: Inconsistent Reaction

Strength ............................................. .6-5

7-1. Troubleshooting Chart: Drive Motor ......................... .7-2

7-2. Troubleshooting Chart: Power .............................. .7-3

7-3. Troubleshooting Chart: Saline Fill ........................... .7-4

7-4. Troubleshooting Chart: Decant .............................. 7-5

7-5. Troubleshooting Chart: Lid Latch ........................... .7-6

10-1. Suggested Spare Parts, CeUwasher 2 ......................... 10-2

10-2. CeUwasher 2 Assembly .................................... 10-3

10-3. Chassis and Component Assembly ........................... 10-7

10-4. Drive Motor Assembly ..................................... 10-13

Rev. n/90 vii/viii

SORVALL® Instruments Cellwasher 2 Service

Section 1. INTRODUCTION

This manual is a service guide for the Sorvall Cellwasher 2 Cellwashing

System. It contains descriptive information; preventive maintenance

procedures; mechanical and electrical theories of operation; applications

information; a troubleshooting guide; field procedures for repairs and

replacements; and an illustrated parts list for ordering replacement

parts.

1-1. Intended Users

This manual has been written for qualified service personnel who are

familiar with factory methods for performing repairs, adjustments and

calibrations. While the descriptive information and operating

procedures contained in this manual are useful to the cellwasher

operator, the replacement and calibration procedures (especially those

involving electrical circuitry) should be performed only by qualified

service personnel.

1-2. Warnings and Cautions

Warnings and cautions appear throughout the manual. Service personnel

are expected to be familiar with their meaning (see page ii) and to

read them before servicing an instrument.

1-3. Service Decontamination Policy

———————————————————————— WARNING ————————————————————————

Either biological or radioactive contamination of the

instrument and/or rotor can occur because of the samples

likely to be processed. Always be aware of this

possibility and take normal precautions. Use appropriate

decontamination procedures should exposure occur.

If an instrument or rotor that has been used with biohazardous material

requires servicing by DuPont personnel, either at the customer's

laboratory or at a DuPont facility, comply with the following procedure

to ensure the safety of DuPont personnel:

• Clean the instrument and/or rotor to be serviced of all

encrusted material, and decontaminate it prior to servicing by

the representative. There must be no radioactivity detectable

by survey equipment.

• Attach a completed Decontamination Information Certificate

(SORVALL Instruments Form No. IPDP-59) to the instrument or

rotor.

Rev. 11/90 1-1

Cellwasher 2 Service SORVALL® Instruments

If the instrument and/or rotor to be serviced does not have a

Decontamination Information Certificate attached, and in DuPont's

opinion presents a potential biohazard, the DuPont representative will

not service the equipment until proper decontamination and certification

is complete. If DuPont receives an instrument or rotor at its Service

facilities which, in DuPont's opinion is a biohazard/ the sender will

be contacted for instructions as to the disposition of the equipment.

Disposition costs will be borne by the sender.

Decontamination Information Certificates are included with these

instructions. Additional certificates are available from the local

Technical or Service Representative. In the event these certificates

are not available, a written statement certifying that the instrument

and/or rotor has been properly decontaminated and outlining the

procedures used will be acceptable.

NOTE

The Service Representative will note on the Customer

Service Repair Report if decontamination was required, and

if so, what the contaminant was and what procedure was

used. If no decontamination was required, it will be so

stated.

1-4. Warranty Responsibility

Whenever service of the instrument is attempted by anyone other than

an employee of DuPont or an authorized DuPont representative, the

individual performing the service is assuming the risk of voiding the

instrument warranty, which is as follows:

E. I. Du Pont de Nemours and Company makes no warranty of any

kind, expressed or implied, except as stated in this warranty

policy.

The Sorvall® Cellwasher 2 Cell Washing instrument and DA-12

Rotor are warranted to be free from defects in materials and

workmanship for a period of one (1) year from the date of

delivery. DuPont will, at its option, repair or replace and

return free of charge any part which is returned to its factory

within said period, transportation prepaid by user, and which

is found upon inspection to have been defective in materials

or workmanship. This warranty does not include normal wear

from use; it does not apply to any instrument or part which

has been altered by anyone other than an employee of DuPont,

nor to any instrument which has been damaged through accident,

negligence, failure to follow operating instructions, the use

of electric currents or circuits other than those specified

on the plate affixed to the instrument, misuse or abuse.

DuPont reserves the right to change, alter, modify or improve

any of its instruments without any obligation to make

corresponding changes to any instrument previously sold or

shipped.

1.2 Rev. 11/90


THE FOREGOING OBLIGATIONS ARE IN LIEU OF ALL OTHER OBLIGATIONS AND

LIABILITIES INCLUDING NEGLIGENCE AND ALL WARRANTIES/ OF

MERCHANTABILITY OR OTHERWISE, EXPRESSED OR IMPLIED IN FACT OR BY

LAW, AND STATE OUR ENTIRE AND EXCLUSIVE LIABILITY AND BUYER'S

EXCLUSIVE REMEDY FOR ANY CLAIM OR DAMAGES IN CONNECTION WITH THE

SALE OR FURNISHING OF GOODS OR PARTS/ THEIR DESIGN, SUITABILITY

FOR USE, INSTALLATION OR OPERATION. DUPONT WILL IN NO EVENT BE

LIABLE FOR ANY SPECIAL OR CONSEQUENTIAL DAMAGES, AND OUR LIABILITY

UNDER NO CIRCUMSTANCES WILL EXCEED THE CONTRACT PRICE FOR THE GOODS

FOR WHICH LIABILITY IS CLAIMED.

If there are any questions concerning the effect of service on the

warranty, contact the nearest representative of Sorvallo Instruments.

1-3/1-4


Section 2. DESCRIPTION, INSTALLATION and OPERATION

This section describes the Sorvall® Cellwasher 2 and provides speci¬

fications, installation information, and instructions for operating

the instrument.

2-1. Description of the Cellwasher 2

The SORVALL® Cellwasher 2 is designed to perform the washing phase of

the Coomb's Procedure automatically. The centrifuge and saline pump

are contained in one cabinet, with the operating controls on the front

panel.

The Cellwasher 2 is microprocessor-controlled, featuring a front panel

with sensor-touch controls and a digital timer for the wash cycle. Other

features include a saline detect system with an audible low-saline

warning and an agitate cycle that ensures complete resuspension of

cells and eliminates manual agitation.

2-2. Cellwasher 2 Specifications

Dimensions:

Width ............

Depth ............

Height, lid closed

Height, lid open .

Mass (Weight)

Electrical Requirements*:

Motor Type

Speed** for 60 Hz models:

Low

High

Decant

Speed** for 50 Hz models:

Low ...................

High ..................

Decant ................

31.8 cm (12.5 in)

35.6 cm (14.0 in)

36.8 cm (14.5 in)

57.2 cm (22.5 in)

16.4 kg (36.0 Ib)

115 V + 10%, 60 Hz, 5A

or

230 V + 10%, 50 Hz, 2A

3 speed induction

1150 to 1200 rpm

3500 to 3600 rpm

600 rpm

1450 to 1500 rpm

2950 to 3000 rpm

600 rpm

(continued)

2-1


Decibel .................................... 67 Db

Length of Modes:

Automatic ................................ Total cycle time 80

seconds

Manual ................................... High or low, optional,

up to 999 seconds if

timed; indefinite if

in HOLD

Tube Sizes:

Diameter ................................. 12 mm + 0.09 mm

or

10 mm + 0.09 mm*

Length ................................... 75 mm + 2 mm

Tube Material: ............................. High strength glass, such

as Pyrex® or Corex®

WARNING

Use only tubes which are guaranteed to be within the

specifications given above. If tubes outside these

specifications are used, test results will be affected,

the rotor and distributor could be damaged, and personal

injury could result.

2-3. Installation Information

a. Location

Place the Cellwasher 2 on a sturdy/ level bench or table near a sink,

drain, or waste container to receive decanted saline. The following

factors should be considered when selecting a location:

• Allow a 15 cm (6 inch) clearance behind the instrument for the

saline pump and tubing.

Allow a 61 cm (24 inch) clearance above the tabletop or bench

surface for the lid to open.

"Adapter Clips (PN 04330) are required when using 10 mm x 75 mm tubes in the DA-12 Rotor.

2-2 Rev. 11/93

SORVALL® Instruments Ccllwasher 2 Service

Drainage in the Cellwasher 2 is accomplished by gravity, so the

drain tubing must extend downward from the instrument to the

drain, sink, or waste receptacle.

The preferred location for the saline supply is either at or

above instrument level.

To obtain the best results, minimize tubing length from the

saline supply to the cellwasher.

CAUTION

Cooling air is drawn into the Cellwasher 2 from all four

sides of the base. Be sure that the entrances are not

obstructed.

If the saline supply reservoir is moved from its original location,

recheck the saline volume by priming the system as explained in

paragraph 2-4, b.

b. Electrical Requirements

The power cord for the Cellwasher 2 has a universal keyed plug that

inserts into a receptacle at the back of the instrument. The other end

must be connected to the appropriate power supply:

115 V + 10%, 60 Hz, 5A or 230 V + 10%, 50 Hz, 2.5A

as specified on the rating plate on the back of the instrument.

————————————————————— WARNING —————————————————————

The receptacle used should be a 3-wire system. If it is

not, the equipment must be grounded to earth to avoid the

possibility of electrical shock.

c. Tubing Installation

The Cellwasher 2 is shipped with the tubing already connected to the

instrument, but not installed in the peristaltic pump. To install the

tubing in the peristaltic pump, unwrap the bundle of tubing at the back

of the instrument and proceed as follows (refer to figure 2-1):

2-3

Cellwashcr 2 Service SORVALL® Instruments

1. Remove the four knurled thumbscrews from the peristaltic pump

and remove the front half of the pump housing. (Do not remove

the pump roller assembly.) Make sure that the pump rollers are

clean and move freely.

2. Turn the roller assembly so that one of the rollers is in the

11 o'clock position.

3. Without turning the rollers, place the pump tubing around the

rollers (the end with the connector should be on the left side) .

4. Pull up on the two ends of the pump tubing, and fit the tubing

into the pump housing around the rollers.

Figure 2-1. Cellwasher 2 Tubing Diagram

2-4 Rev. 4/96


Table 2-1. Parts Location: Tubing Diagram

(key to figure 2-1)

Item Description

I*

2

3

4

5

6

7

8

9

10

Overflow Tubing 15 cm (6 inches) long

Vent Tubing 15 cm (6 inches) long

Pump Tubing 25 cm (10 inches) long

Flow Tubing (to nozzle) 30 cm (12 inches) long

Discharge Tubing 20 cm (8 inches) long

Supply Tubing (to pump) 60 cm (24 inches) long

Drain Tubing 121 cm (48 inches) long

Pump Connector

Y-Connector

Adjustable Tubing Clamp

NOTE

Do not use a sharp object to fit the pump tubing in place

as this could puncture the tubing.

5. With the pump tubing in place, replace the front half of the

pump housing. Stretch the ends of the tubing while fitting the

pump housing in place to eliminate slack in the tubing.

6. Make sure that the halves of the housing are evenly mated. A

gap indicates that the pump tubing is pinched; if so, remove

the front half of the housing and repeat steps 3 through 6.

7. Secure using the four thumbscrews (removed in step 1) and hand

tighten.

8. Check the pump tubing installation by turning the roller

assembly shaft counterclockwise using pliers (should rotate

smoothly without pinching).

9. Install the flow control valve as follows:

a. Cut a length of tubing 60 cm (24 inches) long to be used as

supply tubing. Attach the supply tubing to the pump connector

on the left side of the pump housing.

* In the event of saline residua] in the rotor chamber (e.g. from overfill of tubes or tube breakage) saline will exit through this tube. Under

normal operating conditions, saline will exit through the drain tubing, not through the overflow tubing.

Rev. 11/93 2-5


b. Place the valve over the supply line at a convenient point

and insert the end of the supply tubing into a saline supply

reservoir.

c. Connect the pump tubing from the right side of the pump

housing to the inlet port of the flow detector.

10.Into the adjustable tubing clamp located to the left of the

peristaltic pump, position the bottom leg of the Y-Connector

(with discharge tubing attached).

11. Holding the Y-Connector in place with one hand, fold the inner

strap over the Y-Connector/discharge tubing. Then, fold the

outer strap over the inner strap and push on the outer strap

to lock in place. You will hear a "click" when the clamp is

secured.

12 .Make sure the drain tubing flows downward to an open sink, drain,

or waste receptacle since drainage in the Cellwasher 2 is

accomplished by gravity. Use the shortest length of drain tubing

possible and make sure the tubing is positioned so there is no

upward travel to result in a "trap" for collected waste fluid.

13.Ideally, the drain tubing should be positioned so that it can

not become immersed in collected waste fluid. However, if there

is a possibility that the drain tubing will become immersed in

waste fluid, cut the drain tubing at a convenient point near

the waste receptacle and insert the Y-Connector. Always insert

a Y-Connector in the drain line when using a waste container

for discharged saline.

NOTE

The Y-Connector prevents pressure from building up in

the line due to submerged tubing or air blockage. Failure

to vent the drain tubing by installing a Y-Connector can

result in improperly decanted samples.

If the saline supply reservoir is moved from its original

location, recheck the saline volume by priming the system

as explained in paragraph 2-4, b.

14.If necessary, use additional tubing and connectors from

the Tubing Kit to supply saline and discharge waste

properly.

2-6 "s"-4/^


WARNING

Saline solution from some manufacturers contains sodium

azide as a preservative. If this solution is discharged

directly down the drain, explosive azide salts may form

as the sodium azide reacts with the plumbing. Check with

your saline supplier before discharging saline from the

Cellwasher 2 into a drain.

CAUTION

All saline solutions have long term corrosive effects.

Routine cleaning and maintenance (as described in Section

3) are essential to ensure safe and efficient operation

of the Cellwasher 2.

d. DA-12 Dual Angle Rotor and Distributor Installation

1. Open the lid of the Cellwasher 2.

———————————————————— WARNING ————————————

All Sorvall® Cellwashers manufactured prior to February

1984 were supplied with a plastic rotating bowl assembly

which required annual replacement. All plastic rotating

bowl assemblies are more than one (1) year old and should

be removed from service and discarded immediately. Failure

to do so can result in damage of equipment and/or personal

injury. Replace the plastic rotating bowl assembly with

a stainless steel rotating bowl assembly (PN 12796).

NOTE

There may be one or more holes in the stainless steel

rotating bowl assembly (PN 12796). These holes are for

balancing purposes only and will not effect the perform¬

ance of the stainless steel rotating bowl.

2. Position the stainless steel rotating bowl assembly over the

motor drive shaft. Align the two drive pins in the bowl with

the slot in the drive shaft (see figure 2-2). Slide the bowl

down the drive shaft until the two drive pins in the bowl engage

the rubber drive ring. Press the bowl down firmly.

2-7


MOTOR DRIVE

SHAFT

SLOT

ALIGNMENT

STAIN LESS STEEL ROTATING BOWL ASSEMBLY

DRIVE PINS

Figure 2-2. Stainless Steel Rotating

Bowl Installation

3. Slide the DA-12 Rotor over the drive shaft and lower it gently

into the rotating bowl.

4. Hold the edge of the rotating bowl and turn the rotor slowly

until the drive pins in the rotating bowl engage the rotor and

the rotor drops down onto the rotating bowl.

5. Place the distributor over the rotor knob (see figure 2-3) . Turn

the distributor until its three pins engage the three holes in

the rotor. Press the distributor down until it is completely

seated on the rotor.

CAUTION

If the stainless steel rotating bowl assembly, DA-12

Rotor, and distributor are not installed properly, damage

to the instrument will result. Before operating the

Cellwasher 2:

Make sure the drive pins in the rotating bowl assembly

are engaged in the rubber drive ring and in the rotor,

and that the distributor is firmly seated on the rotor

with all three distributor pins engaged in the rotor (see

figure 2-3).

2-8


DISTRIBUTOR

CANNULA

DISTRIBUTOR PIN

ROTOR KNOB

DUAL ANGLE ROTOR

Figure 2-3. Distributor Installation

e. Rotor Loading and Balancing:

The Cellwasher 2 can process any balanced load up to twelve tubes. When

using less than a full complement of tubes, place the tubes in opposing

compartments to ensure that the rotor is symmetrically balanced. The

volume of saline delivered through the distributor will be the same/¬

at the locations without tubes, saline will be centrifuged into the

basin and drained off.

WARNING

Use only tubes which are guaranteed to be within the

specifications given in paragraph 2-2. If tubes outside

these specifications are used, test results will be affected,

the rotor and distributor could be damaged, and personal

injury could result.

Rev. 11/93 2-9


2-4. Cellwasher 2 Operation

a. Controls and Indicators

All controls and indicators for the Cellwasher 2/ except the power

switch, are located on the front panel keyboard. The sensor-touch

controls register commands visually and/or audibly.

Power to the instrument is controlled by a switch located in the lower

right hand corner of the cellwasher, beneath the keyboard. The symbol

"1" indicates ON while the symbol "0" indicates the OFF position.

Switching the power off erases any manually programmed spin times from

the memory.

Table 2-2 describes the controls and indicators on the front panel.

Figure 2-4 shows their locations on the keyboard.

20 19 18 16 17 15 14 13

Figure 2-4. Cellwasher 2 Controls and Indicators

*LED, or light emitting diode, refers to the small amber light adjacent to the panel button.

2-10


Table 2-2. Cellwasher 2 Controls and Indicators

(key to figure 2-4)

Item Name Acceptance Signal Function

AUTO LED* lights up,

accompanied by one beep.

HIGH LED* lights up,


Selects AUTO run

mode for automatic

wash cycle.

Selects HIGH speed

run for duration

of time selected.

LOW LED* lights up,


Selects LOW speed

run for duration of

time selected.

4 CHECK LED* lights up,

accompanied by one beep,

When in AUTO mode

only, stops at the

end of any step in the

wash cycle to allow

verification of proce¬

dure.

CYCLE

CYCLE

DISPLAY

Panel key.illuminates,

digital cycle display


Selects 1, 2, 3 or 4

wash cycles during

the AUTO mode.

Displays the number

of wash cycles se¬

lected in the AUTO mode.

7 TIME

DISPLAY

Displays the time

selected for AUTO,

HIGH or LOW mode in

seconds.

TIME Digital time display,

accompanied by one

beep.

In HIGH or LOW mode

only/selects duration

of centrifugation time

in seconds.

START LED* lights up,


Starts the mode

selected.

'LED, or light emitting diode, refers to the small amber light adjacent to the panel button.

2-11



(key to figure 2-4, continued)


10 STOP LED* lights up,


Stops the spin in

progress. In AUTO,

HIGH, or LOW mode,

the program will return

to conditions at the

start of the run.

11 PRIME Single beep. Primes the pump for

6 seconds.

12 AG Single beep. Agitates the rotor for

5 seconds to resuspend

cell

button.

13 CLEAR Single beep. In HIGH or LOW mode

only, clears digital

display so that time

can be reset.

14

15

ALARM

OFF

STEP

16 HOLD Single beep.

17 ALARM Single beep, ALARM

ON lights up.

18 SALINE SALINE lights up.

In AUTO mode only,

turns off the alarm.

In AUTO mode only, by¬

passes one step at a

time in the wash cycle

for each push

of the button.

In HIGH or LOW mode,

sets the centrifuge to

spin indefinitely.

In AUTO mode only,

activates an alarm that

sounds at the

end of the cycle.

Lights up when saline

supply is interrupted

during AUTO fill.


2-12



(key to figure 2-4, continued)


19 LID LED* lights up.**

20 ALARM ALARM lights up.

Allows the lid latch

mechanism to be opened

at the end of

a run.

In AUTO mode only/

lights up to indicate

the alarm will sound at

the end of the run.

b. Priming the System

The Cellwasher 2 must be primed using the following procedure at

installation, and whenever the saline supply reservoir or the tubing

is changed.

NOTE

When the saline flow is interrupted, prime the Cellwasher 2 as

described in paragraph 2-4 d, SALINE alarm.

1. Open the lid by pressing the LID button,

2. Select: MODE CYCLE TIME CMD

3. Holding a graduated cylinder under the saline dispenser in the

lid, press:

MODE CYCLE TIME CMD

NOTE

If air bubbles remain in the tubing, repeat the PRIME

command. Air bubbles may cause uneven distribution of

saline in the test tubes.


"The Cellwasher 2 can only be opened when the lid LED is illuminated.

2-13


4 . Once air bubbles have been removed, empty the graduated cylinder

and repeat step 3 to determine saline volume (recommended fill

volumes are 54 ml for 12 mm tubes, 43 ml for 10 mm tubes) . If

the volume is not correct, rotate the flow control valve to

increase or decrease the flow of saline.

5. Repeat steps 3 and 4 until the correct volume is obtained.

NOTE

If the saline supply reservoir is moved or replaced, the

fill volume should be rechecked. To ensure proper cell

washing, check saline volume daily.

c. AUTO Mode

The basic operating sequence in the AUTO mode is as follows:

1. Press the LID button to open the lid.

2. Install rotor, tubes and distributor as explained in paragraph

2-3, d and e.

3. Close the lid.


5. For an audible signal at the end of the run, press ALARM ON.

6. Press START to begin the run.

7. At the end of the run, press ALARM OFF to turn off the audible

signal.

8. Once the lid LED is illuminated, press LID to open the lid.

NOTE

To ensure proper fill, press CHECK after beginning the

first run. At the end of the fill step, the Cellwasher

2 will stop. Examine the level of saline in each tube.

Fill is generally considered adequate if:

2-14


• the level of saline in all tubes is visible above the

metal bands holding the tubes,

the difference between maximum fill and minimum fill

is less than 0.7 ml/ or 3/8 inch for 12 mm tubes and

7/16 inch for 10 mm tubes.

Press START to continue the run.

d. SALINE alarm: this alarm will sound during the filling step if the

saline supply is interrupted. To deactivate the alarm and correct

the problem, prime the Cellwasher 2 as described on the next page.

1. Open lid (press LID button).

2. Holding a- graduated cylinder under the saline dispenser in the

lid/ press the CMD (command) PRIME button.

NOTE

The SALINE ALARM will not shut off until the PRIME button

has been pressed.

3. Press START to continue the wash cycle in progress.

NOTE

The SALINE alarm may also indicate broken or obstructed

tubing. If the saline reservoir is adequate, check the

tubing. Clean or replace tubing if necessary (see Section

3, Maintenance).

To ensure adequate washing, perform one additional wash

cycle at the completion of the cycles in progress.

During AUTO operation, the following features may also be used:

• CHECK button - the automatic wash cycle can be checked after

any step of the cycle (i.e., fill, spin, decant) by pressing

the CHECK button during that step. The Cellwasher 2 will

automatically stop at the end of the step in progress and the

lid may then be opened. After checking the tubes, close the

lid and press START to continue the run.

2-15


STEP button - if the AUTO mode is interrupted by a STOP command,

it will restart at the beginning of the programmed run. To move

the sequence ahead (to avoid refilling tubes for instance) press

the STEP button once for each step in the cycle you wish to

bypass.

STOP button - the run in progress can be stopped immediately

by pressing the STOP button. When the LID light goes on/ the

lid may be opened. When the instrument is restarted, it will

begin the programmed cycle over again unless steps are bypassed.

e. HIGH and LOW Speed Mode

1. Press the LID button to open the lid.

2. Install rotor, tubes/ and distributor as explained in paragraph

2-3, d and e.


1.999| STAR-H

SECONDS

NOTE

The spin time programmed into the Cellwasher 2 when

operating in the HIGH or LOW mode remains in the memory

of that mode until a new time is selected by the operator

or until the power is switched off. After pressing HIGH

or LOW, the time selected for the previous run will appear

in the digitial time display.

4. After the start button is pressed/ agitation will occur

automatically for 5 seconds before the run begins. The time

will count down in seconds.

5. At the end of the run/ press LID to open the lid.

NOTE

Depending on run conditions and component variability from

instrument to instrument, the rotor may still be spinning

at low speeds for a few seconds after the lid is opened.

2-16

SORVALL® Instruments CeUwasher 2 Service

6. During HIGH or LOW speed operation, the following features may

also be used:

• AGITATE - pressing the AG button when the rotor is not

spinning/ will provide an additional 5 seconds of agitation.

2 HOLD - after selecting HIGH or LOW mode, press HOLD to provide

an indefinite spin period. The run will continue until the

STOP button is pressed.

2-5. Emergency Sample Recovery

If the main power shuts off because of a power failure or system

malfunction while the rotor is spinning, the Cellwasher 2 lid will not

open. A mechanical override is provided to allow sample recovery in

the case of an emergency. This procedure should never be used routinely

and is intended for emergency sample recovery only.

WARNING

When the main power shuts off, the brake will not operate.

Wait until the rotor stops spinning before using the

mechanical override. Reaching into the rotor chamber

before the rotor has stopped spinning could cause personal

injury.

The mechanical override is located on the left side of the Cellwasher 2 .

Depending on your instrument, the override is located in either the

lid or the side of the cabinet as shown in figure 2-5. If the mechanical

override is located in the lid, insert a small screwdriver or similar

object into the hole and push; if it is the small pinhole located in

the side of the cabinet, insert a straightened paperclip and push. The

lid will open.

2-17

Ccllwasher 2 Service SORVALL® Instruments

HOLE FOR

MECHANICAL OVERRIDE

HOLE FOR-

MECHANICAL OVERRIDE

HOLE IN LID (CURRENT LOCATION) PINHOLE IN SIDE (PREVIOUS LOCATION)

Figure 2-5. Location of Mechanical Override

2-6. Condensed Operating Instructions

The Condensed Operating Instructions appended at the end of this

section are provided as a reference for the operator who is familiar

with the Cellwasher 2. Refer to the complete operating instructions

in the Cellwasher 2 Instruction Manual before using the centrifuge for

the first time.

2-18

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2-19/2-20


Sections. MAINTENANCE

This section describes routine maintenance procedures for the

Cellwasher 2 that should be performed on a regular basis. It is the

customer's responsibility to make sure that these procedures are

followed when necessary. If further maintenance is required, it should

be performed by a representative of DuPont or other qualified service

personnel.

WARNING

Because tampering with high voltage electrical circuits

can cause severe electrical shock/ untrained personnel

must not attempt to test or repair any electrical circuits

in this instrument.

3-1. Inspection and Cleaning

The following preventive maintenance checks and cleaning procedures

should be performed daily. If etiologic or biohazardous materials are

processed in the Cellwasher 2, be sure appropriate decontamination

procedures have been followed before inspecting or cleaning the

instrument.

a. Daily Inspection

Check all sealing surfaces, tubing, liners, and the collecting

ring assembly for cleanliness and good condition. Replace any

worn or defective parts. Replace tubing if it is cracked or

contains accumulated material.

• Inspect the screen on the bottom of the Cellwasher 2 and remove

any obstructions.

• Inspect the stainless steel rotating bowl assembly as follows:

1. Remove the rotor and distributor.

2. Remove the rotating bowl assembly located in the rotor

chamber, directly beneath the rotor.

3 . Inspect the rotating bowl assembly for any sign of corrosion.

Replace if necessary.

NOTE

If inspection reveals that any part of the Cellwasher 2

is not functioning properly, do not use the instrument

until it is repaired.

3-1


WARNING

To ensure safe and efficient operation of the Cellwasher

2, all plastic rotating bowl assemblies must be replaced

with a stainless steel rotating bowl assembly (PN 12796).

All Sorvallo Cellwasher 2/ s manufactured prior to February

1984 were supplied with a plastic rotating bowl assembly

which required annual replacement. All plastic rotating

bowl assemblies are more than one (1) year old and should

be removed from service and discarded immediately.

Failure to do so can result in damage of equipment and/

or personal injury.

b. Cleaning

CAUTION

All saline solutions have long term corrosive effects.

Routine cleaning and maintenance (as described in this

section) are essential to ensure safe and efficient

operation of the Cellwasher 2.

Clean the cabinet daily with a damp cloth and mild detergent.

Wipe up spills from the interior and exterior of the cellwasher

whenever they occur.

Remove the rotor, distributor, and stainless steel rotating

bowl assembly and wash them daily with warm water and mild

detergent.

Remove the collecting ring assembly and tubing for routine

cleaning as follows (refer to figure 3-1 for instruments serial

number 8702302 and higher; or figure 3-2 for instuments serial

number 8702301 and lower):

1. If on the back of your instrument, there are tubing clamps

and a T-Connector or a Y-Connector (refer to figure 2-1):

open the adjustable tubing clamp that secures the T-Connector

or Y-Connector in place and remove the discharge tubing.

2. Remove the four mounting screws and step washers fastening

the collecting ring assembly to the lid.

3 . Pull the collecting ring assembly away from the lid, and pull

the discharge tubing up through the wet guard (the molded

black liner).

4. Disconnect the flow tubing from the spray nozzle in the lid

and pull it through the hole in the retainer ring.

3-2


5. Separate the collecting ring assembly by pulling the

collector ring off.

6. Remove all pieces of tubing, wash with warm water and mild

detergent/ and reinstall tubing.

7 . Wash all components of the collecting ring assembly with warm

water and mild detergent.

TO FLOW

DETECTOR

STEP WASHER

MOUNTING

SCREW

TO WASTE

CONTAINER OR

T- OR Y-CONNECTOR

Figure 3-1. Exploded View, Collecting Ring Assembly

(Serial Number 8702302 and higher)

3-3


NOTE

Make sure the nozzle for the discharge tubing on the

collector ring will align with the groove in the wet guard

when the assembly is reinstalled in the Ceilwasher 2.

8. Reassemble instruments serial number 8702302 and higher as

follows:

a. Place the retainer ring on a table or bench so that it

rests on the smaller opening, and work the 0-ring seal

around the outside of the large opening until it is fully

seated in the groove of the retainer ring. Then, place

the collector ring so that it rests concentrically over

the 0-ring seal and press firmly into place. Then proceed

to step 10.

9. Reassemble instruments serial number 8702301 and lower as

follows:

a. Place the retaining ring on a table or bench so that it

rests on the smaller opening. Lay the vinyl seal

concentrically on the upper edge of the retainer, and

gently place the collector ring on the seal so that it

also rests concentrically.

b. Place the thumb of each hand on top of the entire

assembly, slip your fingers beneath the lip of the

retaining ring, and begin pressing the two rings together

evenly all around the lip. Lift the entire assembly and

continue to squeeze the rings until both edges are evenly

together.

c. Examine the assembly: at no point along the edge should

more than 6_mm (0.25 inch) of the vinyl seal protrude.

If it does, open the entire assembly and realign the seal.

Once the vinyl seal is centered, any excess less than 6_mm

may be trimmed off with a sharp knife or blade. Then

proceed to step 10.

10. Reinstall the collecting ring assembly in the lid of the

Cellwasher 2 by reversing steps 1 through 4 of this

procedure.

NOTE

When replacing the four mounting screws, make sure the flat

side of each step washer faces the screw head.

3-4


Figure 3-2. Exploded View, Collecting Ring Assembly

(Serial Number 8702301 and lower)

3-2. Tubing Replacement

If tubing is damaged and must be replaced, install tubing supplied in

the Tubing Replacement Kit (PN 12977) following the instructions

supplied with the kit. Refer to paragraph 9-1 for a copy of the tubing

installation instructions.

3 - 3. Cellwasher 2 Preventive Maintenance Procedure

NOTE

Before starting the preventive maintenance procedure, be

sure the instrument is functioning properly.

3-5


a.Visually inspect the Cellwasher 2 for cracks/ saline leaks and

corrosion (including the stainless steel rotating bowl, saline

detector, motor, pump and pump motor).

b. General Inspection

Inspect and/or check the following:

• Touch Panel - check all touch switches for proper function.

• Nozzle Assembly - check for cracks, leaks and obstructions.

Drive Ring - check for elongated holes/ softness and loose set

screws; check proper height dimensions.

Drain Tray - should be clean with no obstructions in drain tube

and drain tray gasket.

• Motor Mounts - check for tightness, separation and softness.

Lid - inspect front seal and mating surfaces.

Hinge and Torsion Bar - check for proper function.

Collecting Ring Assembly - check condition of seal.

• Motor and Decant Coil - check for resistance, coil concentricity

and saline deterioration.

• Electrical Connections - check for looseness and worn wires.

• Pump - inspect for rusty or noisy rollers.

• Saline Detector - inspect and check function.

• Tubing - check all tubing for cracks and/or obstructions.

c. Inspect all tubing; replace if necessary.

d. Lid Latch

1. When the lid is closed, the latch engaged, the lid light not

illuminated and the instrument operating, the lid can not be

opened.

2. With the lid open and the lid light illuminated, the instrument

will not run except in the agitate prime mode.

3. Upon completion of a run, the lid can not be opened until the

lid light illuminates.

3-6 Rev. 11/90


4. With the lid closed and latched, turn the power switch to off.

Locate the hole in the lid for the mechanical override. Insert

a small screwdriver or similar object in the opening to open

the lid (refer to figure 2-5).

e. Rotor

Inspect the DA-12 Rotor as follows:

The open fingers should have a minimum spacing of approximately 0. 015

inch. The centerpost should be perpendicular to the baseplate with the

tube holders straight. (The tube holders should swing freely).

f. Distributor

Inspect the distributor for bent or clogged cannulas. Check the nylon

washer and spring clip for proper insertion and function.

g. Stainless Steel Rotating Bowl Assembly

Inspect the stainless steel rotating bowl assembly for corrosion.

Replace if necessary.

WARNING

To ensure safe and efficient operation of the Cellwasher

2, all plastic rotating bowl assemblies should be removed

from service and discarded immediately, and replaced with

a stainless steel rotating bowl assembly (PN 12796).

Failure to replace the plastic rotating bowl assembly can

result in damage of equipment and/or personal injury

should the plastic rotating bowl fail.

h. Time

Check the time at the following functions, using a stopwatch:

1. Select LOW mode; key in 10 seconds; push START. The cellwasher

will begin to agitate. When agitation stops, begin timing the

10 seconds.

2. Select AUTO mode; select 1 cycle; push START. Time the following

portions of the cycle:

• Fill (pump time) ............. 6 seconds

3600 rpm spin ................ 47 seconds

Decant spin .................. 4 seconds

• Agitate ...................... 5 seconds

Rev. 11/90 3-7


i. Speed

Install a rotor and check the speed using a hand tachometer, with a

shaft extension. Check speed at:

• LOW

60 Hz ........................ 1200 +0/-50 rpm

50 Hz ........................ 1500 +0/-50 rpm

• DECANT ......................... 600 ± 25 rpm (50 and 60 Hz)

. FILL AND HIGH

60 Hz ........................ 3600 +0/-100 rpm

50 Hz ........................ 3000 +0/-100 rpm

j. Total Volume

Check volume of saline by holding a graduated cylinder under the saline

dispenser in the lid and pressing PRIME. Recommended total volume is

54 ml for 12 mm tubes/ 43 ml for 10 mm tubes. Adjust flow control valve

until total volume is adequate.

k. Run System

1. Check and run system with fully loaded rotor.

2. Check and run system with six (6) tubes.

3-8 Rev. 11/90

SORVALL® Instruments Ccllwashcr 2 Service

Section 4-1. MECHANICAL THEORY

This section describes the mechanical functions of the Cellwasher 2.

4-1. Controls

The Cellwasher 2 control panel features touch pad speed switches and

function selectors, a digitized electronic timer/ and the choice of

either automatic or manual operation. Refer to Section 5, Electrical

Theory, for detailed operation.

4-2. Flexible Drive Mount-ing

The motor and rotor combination is vibration mounted using a three point

suspension system. This arrangement isolates minor tube imbalances

from the instrument.

4 - 3. Cabinet

The cabinet is cast from structural foam in one continuous integral

piece for strength and a pleasing cosmetic appearance. The lid is opened

by pushing the LID button to release the solenoid at the completion

of a run; a torsion bar built into the structure raises the lid.

4-4. Air Flow

Cooling air is drawn in from underneath the cabinet/ taken up through

the stainless steel screening, and circulated by the motor fan through

and around the motor and components. The source of air supply must never

be obstructed by objects placed in front of or near the base of the

instrument.

4-5. Motor

The motor has been specially wound to operate at either 3600 (60 Hz),

3000 (50 Hz), 1200 (60 Hz), 1500 (50 Hz) or 600 rpm depending on operator

selection or program sequence. Dynamic braking is accomplished by

applying current to the motor to retard its rotation.

4-6. Safety Latch

When the lid is closed on the cabinet, a solenoid plunger automatically

penetrates the lid, thus securing it. To make sure the lid has been

closed and the plunger is in place, two signals are generated indicating

whether or not lid-locking conditions have been met before permitting

the Cellwasher 2 to operate. Two hall effect transistors have been

placed on the lid latch PC board to ascertain that the lid is closed

and that the plunger is extended in position. The lack of either signal

will prevent operation.

4-1


4-7. Saline Pump System

The quick load tubing pump offer the advantages of a unique, easy method

of loading the tubing. The pump uses stainless steel rollers and has

a clear plastic housing, both of which offer excellent protection

against corrosion. The pump is driven by a shaded pole AC drive motor

gear reduction head to minimize pump tubing wear.

4-8. Flow Detector

This device is placed between the saline pump output and the

distribution nozzle to detect interruptions in saline flow. The

detector is mounted vertically so the saline causes an upward

displacement of a magnet in the path of the saline stream. When saline

flows properly/ a magnetic reed switch is open and no signal is

generated. When saline flow decreases, the magnet displacement is

minimal and the reed switch is in the ON position for signal generation.

This signal warns the operator of insufficient saline supply to the

instrument.

4-9. Flow Control Clamp

The flow control clamp restricts the flow of saline through the tubing.

4-10. Motor Antirotation Clutch

This clutch is mounted on the bottom of the drive motor and allows one

rotation only with no back lash. Roller bearings wedge themselves up

an inclined plane when the rotor is turned in the opposite direction,

preventing further rotation of the motor.

4-2


DUPONT CELLWASHER 2

Preventive Maintenance Checklist

SN

ACCOUNT

DATE

SR

OK ADJ

D D

D D

D D

D D

D D

D D

D D

D D

D D

D D

D D

D D

D D

OK ADJ

D D

D D

D D

D D

D D

General Inspection

Touch Panel

Nozzle Assembly

Drive Ring

Drain Tray

Motor Mounts

Lid

Hinge and Torsion Bar

Collecting Ring Assembly

Motor Decant Coil

Electrical Connections

Pump

Saline Detector

D D Rotor Bowl Assembly

D D Distributor Inspection

D D Inspect Tubing

D D Replace if requested

Lid Latch

Cannot be opened when instrument is

running.

Centrifuge will not start when lid is up.

Cannot be opened until light illuminates.

Mechanical override.

D D Time

Rll (pump time)

3600 rpm

Decant

Agitate

9 sec

47 sec

6 sec

5 sec

a D Speed

Fill and low

1200+0/-50 rpm

Decant 600+/-25 rpm

High3600+0/-100rpm

D [3 Total Volume

12mm

10mm

D D Fill Level

12mm 3/8" or 0.7 ml

10mm 7/16" or 0.7 ml

D D Run System

D D Full Rotor

D D Six Tubes

Rev. 4/96 3-9/3-10


Section 5. ELECTRICAL THEORY

5-1. System Description

a. General

AC power enters the instrument through the voltage selecting the RFI

filtering (CORCOM) connector, which is at the back of the instrument.

The connector contains a fuse.

The electronics system consists of a +5V power supply and 4 printed

circuit (PC) boards, namely: the touch switch panel / the microcomputer,

the triac-interface, and the latching PC boards. The first three boards

reside in the electronics enclosure to the front of the instrument,

while the latching board is part of the latching mechanism screwed to

the instrument cabinet. A 20-pin flex cable soldered to the touch switch

panel PC board mates to a connector on the microcomputer board. A 16-

pin interconnecting ribbon cable mates to sockets on both the

microcomputer and interface PC boards. Power to and from the

electronics enclosure leaves via a 16-pin molex connector soldered on

the interface board and a connector mounted to the kydex cover housing

the electronics. For more detailed information/ refer to figure 5-1,

System Schematic and figure 5-2, Wiring Diagram. The power supply is

composed of a 115V: 10 VAC center tap transformer, a full wave rectifier,

a filter capacitor and a 5V regulator — all mounted on the instrument

chassis. An unregulated 14 VDC ± 10% should flow across the capacitor.

b. Touch Switch Panel

The touch switch panel contains 25 SPST normally open switches. Twenty-

four of the switches are arranged in a matrix of 4 rows by 6 columns.

The 6 columns: MODE, CYCLE/ TIME 1, TIME 2, TIME 3, and COMMAND are

each scanned by the microcomputer. Data from each column is paralleled

and read by the microcomputer through lines A/ B, C, and D. Because

of the sequential nature of scanning, data can only be received from

the particular switch column being scanned. The one remaining switch

(LID) is independent of the matrix arrangement, and is the only switch

with audio feedback. This switch is in parallel with the lid indicator

and in series with the open line which activates or deactivates the

latching mechanism.

The touch switch panel also contains 7 light emitting diodes (LED's),

the state of which are controlled by the microcomputer through lines

labelled START, STOP, AUTO, HIGH, LOW, CHECK/ and LID.

5-1/5-2

~~~~~~~~ Instruments ~~~~~~~~~~ 2 Send~e

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————————————————————~3~———————————— ~~

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System Schematic, Cellwasher 2

5-3/5-4

Rev. 4/96

~~~~~~~~ Instruments ~~~~~~~~~~ 2 Service

POWER ~~RA~S¬ FORMER

~~~~12345

~ ~~ ~ ~~ ~

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Figure 5-2.

Wiring Diagram, Cellwasher 2

5-5/5-6

Rev. 4/96


c. Microcomputer PC Board

The microcomputer PC board contains all of the system's intelligence.

In response to input of commands from the touch switch panel, the

microcomputer makes decisions as to which indicator, seven-segment

display, coil or motor to turn on or off. The components on this board

are located and described in figure 5-3 and table 5-1 and indepth

electrical information is given in figure 5-4, Microcomputer PC Board

Schematic.

The 3870 microcomputer (Z4) is a one chip combination of processor and

memory, with four I/O ports of eight bits each.

^

Figure 5-3. Microcomputer PC Board,

PN 04527, Revision 4

5-7


Table 5-1. Microcomputer PC Board

Component Identification (PN 04527 Revision 4)

(key to figure 5-3)

Component

Designation Description

Manufacturer/s

Order No.

J3 Socket Integrated Circuit

Dip, Solder Tab, 16-pin

Circuit Assembly

Corporation

#CA-16S-10SD

J5

Rl, R2,

R3

R4

R5

R6

R7, R8,

R9, RIO,

Rll, R12,

R13

Connector, Flex-Strip Cable,

20-position

Resistor, Carbon Comp./ 5%,

1/4 W, 1k OHM

Resistor, Carbon Comp./ 5%,

1/4 W, 6.2k OHM

Resistor, Carbon Comp., 5%,

1/4 W, 47 OHM


1/4 W, 4.3k OHM


1/4 W, 100 OHM

T&B Ansley

#742-20

Cl Capacitor, Ceramic Disc,

.1 MFD, 50 WVDC

Centralab

#CK104

C2 Capacitor, Electrolytic,

Aluminum, Radial, 10 MFD,

25 WVDC

Illinois

Capacitors

#106RLR025M

C3

C4

Capacitor, Ceramic Disc,

.01 MFD, 500 WVDC

Capacitor, Electrolytic,

Aluminum, Radial, 47 MFD,

35 WVDC/ -10%, +50%

Sprague

#19C241A2

Sprague

#503D476F035NB

C5

Zl

Capacitor, Metallized

Polyester, 100 VDC, 1.0 mf

Integrated Circuit, Quad

2-Input Positions, Nand Gate

Mepco/Electra

#C280AH/PIM

Texas Instruments

#SN7400N

'Indicates standard component available through most suppliers.

5-8

SORVALL R

Instruments Ccllwasher 2 Service



(key to figure 5-3) continued

Component


Manufacturer/s

Order No.

Z2, Z5

Z3

Z4

Z6

Z7

Z8

Z9

Z10, Z19

Zll

Z12

Z13

Z14

Z15, Z16,

Z17, Z18

Yl

Integrated Circuit/ High

Voltage, Output/ 14-pin/ Dip

Integrated Circuit, Digital

Monostable Multivibrator

Gates

Integrated Circuit, Hex-Non-

Inverting, 3-State Buffer

Integrated Circuit, Darlington

Transistor Array

Integrated Circuit, Cos/Mos

Hex Buffer Converter,

Inverting Type

Resistor, Network, 8-pin Sip

2k OHM, 2.1 Watts

Integrated Circuit, General

Purpose High Current

Transistor Array

Resistor Network, 14-pin Dual

In-Line Package, 390 OHM

Resistor Network, 14-pin Dual

In-Line Package, 47 OHM

Light Emitting Diode, 430 inch,

7 Segments, Red

Crystal, PC Type, 4 MHZ

Texas Instruments

#SN7417N

Texas Instruments

#SN74121N

Microcomputer Programmed Memory

CW-2 04555

CW-2L 04931

Integrated Circuit, BCD to 7 Motorola

Segment Latch/Decoder/Driver #MC14511B

Integrated Circuit, BCD to Motorola

Decimal Decoder/Binary to #MC14028B

Octal Decoder

Motorola

#MC14503B

Sprague

#ULN 2003A

Motorola

#MC14049BCP

Dale Electronics

#MSP08C01

Sprague

#ULN 2082A

Alien-Bradley

#314B391

Alien-Bradley

#314B470

Hewlett Packard

ftHDSP-3733

Crystek

#CY3A

Rev. 4/96 5-9





Component


Manufacturer's

Order No.

BZ1

X24

XZ15, XZ16,

XZ17, XZ18

DS1, DS2

Buzzer, PC Board Mtg,

Socket/ Integrated Circuit,

Low Profile, 40-pin

Socket, Integrated Circuit/

Elevator, Bifurcated

Contacts, 14-pin

Lamp, Indicator, Yellow,

5.0 Volts

Projects

Unlimited

#X30W05

Texas Instruments

#C84-40-02

Aries

#14-8620-10

Penn Keystone

#7561-4-5

TP1, TP2 Terminal, Swage/ 2 Turrett,

Brass, Silver-Plated

H.H. Smith

#2018C

5-10


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————————————~~~ ~~~~~~~ 1——————————————————————————————————————————————~~ ~ ~~~~~

~ ~~ ~ ~ ~~ ~~7

10 1~ ~ ~ ———————————————~~~~2———————————————————————————————————————————————————————~~~ ~

~~~ 100

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10

———————————————————————————————————————————————————————————~———————————~~~5 ~~~,

(~~~~~4~ ~~ ~ ~—— ~~~~

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w

~~

~~

————————~~ ~~~~~~~~ ———————————————————————————————————————————————————————————————————~ ~~ ~~~~~~ ~~

~———————S~~~~~~~~~———————————————————————————————————————————————————————————————~ LID

+5~~ 1 ~/~ ~~ ~ ~

~ ~~~ ~ ~ ~ ~ - ~~~ - -

~~ 1 ~2 ~~~ 3 ~ ~ ~ ~ ~ ~

~ ~//...~b ~~~~~~~~ 13~~~ ~~~ ~~ ~ Is I~~ ,~ ~~~ ~~ ~~

~~ ~

~ ~

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~~~~ ~ ~~~~~~ ~~ ~~~ ~ ~~ ~~ ~~ a

~~~ 7 ~ ~ ~~~~ a ~~~~ ~ ~~ a ~~~~~~~

~ ~~ 4 ~o ~~~ 5 ~ ~

~ ~~~ ~ ~~ ~~~ 7 37~~ 7 ~~~~ 7 37~~

~~ ~—~~~~~~~~ ~~~~ ~ ~~~ ~~~ ~~ ~~ ~ ~~ - ~~~ ~ 10 1 13 ... ~ 9 ~~ ~~ ~~ ~~ ~~ ~~

~~~ ~

~~ ~~ ~ 3 ~~| ~ ~~~~~ ~3 ~~1 ~~ ~S~~ ~ .1———1 ~~——1 ~———I

~~ ~~

~~~ D~~~ ~~~~~~ ~~~~~~~

~~~ ~ ~~~~~ 1 1 ~ ~ ~

A~~~~~ ~~ ~ ~~J

S~LINE

~~~~~~

—————————————————~———~———————————————————————————————————————————————————~——~~~/~ LID ~~

~~~~~ ~1~~~ ~ (3~9

—————————————————————————————————————————————————————————————————————~6 5~5 C

~~~————————————————————————————————————————————————————————————————————~~~3 ~~~ ~~ ~049

~ ~~ ~~

• ~ ~~T ~~

~~~——~~~ ~5~~

~~~~ ~~~~

~~~~~~ 5-4. Schematic,

Microcomputer PC Board

(~~~£)

5-11/5-12


d. Hardware

Six bits of port 0 are dedicated to the LED indicators of the touch

switch panel. The hex buffer gate (Z5) serves to sink current from the

LED's. The seventh bit is used to turn on the alarm lamp via one of

the transistors of Z9 . The remaining bit is used to enable or disable

Z8.

The common cathode 7-segment displays (Z15-Z18) are multiplexed.

Segment data comes from the upper four bits of port 1, through the BCD

to seven segments latch-decoder/driver (Z6), and the high current

(source) transistor array (Z12) . Data from the lower four bits of port

1 act as the display digit selector; through the BCD to decimal decoder

(Z7) and the high current (sinking) transistor array (Z9).

Port 1 is not entirely dedicated to the displays because of the

multiplexing arrangement. The lower four bits of port 1 (used to select

display digits) are also used to scan, via Z7, the six columns of the

touch switch panel. The data from the touch switch panel A, B, C and

D comes in through the noninverting three-state buffer (Z8) and reads

into the microcomputer through the upper four bits of port 1. Z8 is

enabled only when its data is being read in. The 10K resistor pack (Zll)

act only as pullups. In port 4, bits 0, 1, and 7 are not used. Bit 6

is used to light the saline lamp via one of the remaining transistors

of Z9. Bit 5 is used to control the lid indicator and to activate the

door open circuitry of the interface boards. For more current

capability, this line uses a transistor in Z9 as a buffer. Besides going

to the lid indicator, the transistor output goes to lid switch 1 on

the front panel and returns at lid switch 2 . Lid switch 1 and lid switch

2 are on the two legs of the lid switch which completes connection to

the open line for latch solenoid control when it is pressed.

An audible signal occurs when the sensor-touch control buttons are

being operated, when the ALARM button is activated, the end of a run

has been completed, or if the saline level is inadequate. A monostable

multivibrator (Z3) controls the duration of the audible signal (beep)

through C4 and R6. These components presently select a duration of 141

milliseconds. Z3 is controlled by a negative going voltage transition

from bit 4 of port 4.

The saline flow detector is a normally closed switch and is open only

when saline is flowing. Two wires are connected to the switch contacts ;

one is connected to ground and the other is connected to "flow" on the

microcomputer PC board. A high on this line indicates good saline flow

as read in by bit 2 of port 4.

Only bits 0 through 5 of Port 5 are used. Their function is to enable

or disable the triacs on the interface PC board that in turn switch

power to different components in the system.

5-13


e. Interface PC Board

This board interfaces the low level logic of the microcomputer PC board

with the high voltage, high current used by the instrument's components

(refer to either figures 5-6 or 5-8 Triac-Interface PC Board Schematic,

PN 04537 Revision 2 or Revision 4 if your instrument is SN 8503865 or

higher. If your instrument is SN 8503864 or below, refer to figure 5-

10 for Interface PC Board Schematic, PN 04537 Revision 1). The

components for the Triac-Interface PC board, PN 04834, are located and

described in figure 5-5 and table 5-2. Components for Triac PC board,

PN 04839 Revision 4, are located and described in figure 5-7 and table

5-3. The components for Interface PC board, PN 04536, are located and

described in figure 5-9 and table 5-4. Low level signals from the

microcomputer PC board: pump, 3600 rpm*/ 1200 rpm**, 600 rpm, brake,

decant and open, serve to turn on or off zero crossing triac drivers

Zl through Z7 respectively.

Example: Pump Circuitry

31, pin 9, goes low to provide a path for current to flow from the

+5V through Rl and the light emitting diode inside Zl. This turns

on the transistor inside Zl, thus allowing current flow from 115V

through R8 and the transistor to the gate (pin 3) of triac Ql. Once

the triac is enabled, 115 VAC shows up on pin 1 of the triac. The

115 VAC at pin 15 of J4 is used to complete power to the pump motor.

The other leg of the power connection to the pump motor is already

connected to neutral.

All the other circuitries are identical. The decant coil and the latch

solenoid require DC voltages which are furnished by rectifires CR1

and CR4. Varistors CR2 and CR3 protect the loads of these circuits

from possible damage caused by applying and removing power.

The purpose of C9 between decant + and decant - is to boost the level

of average DC voltage reaching the decant coil. This function is

particularly critical at low line voltages. R29 limits current to

C9.

The other purpose of the interface board is to serve as a transfer medium

for the flow, switch 1 latched, and switch 2 latched signals, which

are inputs to the microcomputer board.

f. Software

The software which controls all instrument operations resides on the

microcomputer chip. For a detailed description, see paragraph 5-4. The

microcomputer scans each switch column of the touch switch panel. Any

switch depression is detected, although illegitimate or inappropriate

commands are ignored. The operator/s choices are acknowledged by a

beep, the display of appropriate values and/or the execution of the

selected function. In some cases the command will be acknowledged, but

not carried out due to a lack of the necessary prerequisites. Example:

if the lid is not latched, the instrument will not start.

•3000 rpm for 50 Hz

"1500 rpm for 50 Hz

5-14 Rev. 11/90


Table 5-2. Triac-Interface PC Board



(key to figure 5-5)

Component


Manufacturer's

Order No.

J2

J4, J20

Rl, R2 / R3 /

R4, R5, R6/

R7

R8, R9,

RIO, Rll,

R12/ R13,

R14

R15/ R17,

R19, R21,

R23, R25,

R27

R16, R18,

R20/ R22,

R24/ R26/

R28

Socket/ Integrated Circuit,

Dip Solder Tab, 16-pin

Connector, PC, Vertical,

Friction Lock, Wafer,

16-position, Male

Resistor, Carbon Comp./

1/4 W, ±5%, 120 OHM


1/4 W, ±5%, 180 OHM

Resistor, Fixed-Carbon Comp.,

1/2 W, ±5%, 39 OHM

Resistor, Carbon Comp.,

1/4 W, ±5%, 1k OHM

Circuit Assembly

Corp.

#CA-16S-10SD

Molex

#09-65-1169

R29

CR1, CR4


2 W, ±10%, 47 OHM

Bridge Rectifier, 1.5A Motorola

#MDA942-5

CR2, CR3 Varistor, 130 VRMS General Electric

#V130LA10A

Zl, Z2, Z3,

Z4, Z5, Z6,

Z7

Cl, C2, C3

C4, C5, C6

C7

Integrated Circuit, Triac

Driver, Zero Crossing/

Opto Coupler

Capacitor, Ceramic Disc/

.01 MFD, 500 WVDC

Motorola

#MOC3031

Sprague

#19C241A2


5-15


Table 5-2. Triac-Interface PC Board




Component


Manufacturer's

Order No.

C8

C9

TP1, TP2


Aluminum Radial/ 22 MFD,

25 WVDC


Aluminum Radial, 22 MFD,

250 WVDC

Terminal, Swage, 2 Turrett,


Illinois

Capacitor

#226RLR025M

Illinois

Capacitor

#226RMR250M

H.H. Smith

#2018C

Figure 5-5. Triac-Interface PC Board

(PN 04834, Revision 0)


5-16


~~~~~

~~12 ~——~~

~~~~~~~ ~~——

~~~ ~~~ ~~—

600 ~~~ 6)——

~~~~~~~~ 3 ~

~~~~~ ~~ ~—~

~~ ~~~~~~~ ~~~—~~

~~ L~TCH~D ~~~——

~~~~ +~~~ ~~2~——

~~~~

~~~~ ~~~~~———

~~~~~

——————— ~~~ ~~~~~~

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~~~~~~~~ ~ ——~————~A~~—————

~~~~ ~~a? ~

~~~~ ~

~

~~5~~~~ ~

2

~~6 ~~~~ ~

~

~~7 ~o ~

~1 Hoc 33s ~

~~ ~~~ 30~1

a~ HOC

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~~4 ~OC ~~3~

~5 ~~~ 202~~~

~~

Hoc~ 3~51

~7 ~OC 30~I

~

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4

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———~~~~~——~~~

~~~ ~~

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4

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~

~~~~~~~~~6 ~

4

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~~~~

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a~~~20 ~O ~~~ J~~ ~~~~~~~~~~~~

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1 ~

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~~~~

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~ ~ ~~ ~~

~~~~

~~ ~ ~~

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I——~~~——————1————~ ~~~

5V~ I

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~

—~~~~~~——1———— ~~~~

~~

~~ ~~?~~~27 ~~7 ~~~~~~~

~~~ 1 ~—~~~~——I~——~——————

~~~

1 ~

-

~~~

~~~ ~~~~~~~ ~~~~~

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~~ ~

~~

~ ~~~~ ~~ ~ 1 ~~ ~ ~ S ~ ~1 ~~ ~ \ \~ ~~~~ ~~

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~ ~ ~ 1~ ~

~~~

~~~ ~| ~~ ~~ ~

~~ ~ ~~ ~~~ ~ S

~ ~~~ ~~ ~ ~~ ~~~ ~

~ ~~~~~~ 1 ~ ~~~~ ~ ~\~ ~

~?6 r~~~~~~~

~~~—~~~——~~~ ~ ~ ~\ \~ ~ ~ ~~~~~~

~~~ ~~s5~~

~ ~~~ ~ ~ ~ ~~ ~ ~1 ~~ ~~ ~\ \~ ~~ ~~ ~~~~ ~

~ ~ ~ ~ ~~~~~~

+s~ ~ rp~

~~——1

~rP2 ~~

~,~5~

~~———

————~~~~~~

~ ~~~~E~

~——~ ~~~

~?~~T~~~~~~

~~

——~~~~

~ ~~~~~~~

~ —~1—

~s~

~~~~~~~~~~~~~

1 ~~

11~~~ 1

~

~

~~

~~2

3

~~

5

4

~~~~

~~~~~~~

~~~~DA9~~~ ~

Y

~~~~~~~~~—~——————

~~~~~~

~ ~~~~~ ~1~~

~~~~

5~ ~~~~H~~~

52 LATCHED

~~(~BI~Z~~

~~~~~~

~~~~~~~~~~~~

~~~~-5)

~~

~~~~~~~~ ~7 ~~~

+1~~9 ~~~~ 1

~~5~

~4

~~~ ~~~~~ +

~~9 ~~~~7~r~~-~~~~

6 ~~~~~~~ -

~~~~ ~~~~~~ ~~~

~~~ ~~~~~~ —

~~.~~~~i~~~ ~~~~~~~~~~~~ ~~~~~~~~~~ ~~~~ ~~~~~ ~~~~~~~~~~ ~~~~~ ~ OH~S, ~~~~~~~s°~~

~~ ~~~ ~~~~~~~~~~~~~ ~~~~~~ ~~~ ~~~~~~~~~~~

~~~~~ ~

Figure 5-6.

Schematic, ~~~~~~~~~~~~~~~ PC

Board ~~~ 04537~ Revision 2)


5-17/5-18


Table 5-3. Triac PC Board Component Identification

(PN 04839 Revision 4)

Component


Manufacturer's

Order No.

Cl, C4, C5

C2/ C3/ C6

J21

Capacitor, 0.1 UF/ 500 V

20%

Capacitor, 0.01 UF/ 500 V

Connector, 16-pin/ STR

Ql, Q2, Q3/ Triac/ 8 Amp, Isolated

Q4, Q5, Q6,

Q7

Rl, R2, R3 Resistor, Carbon Comp.,

10 Ohms 1/2 W, 5%

Sprague

#41C92A10

Molex

#26-60-1160

Mitsubishi

#BCRBPM-8L


flSSY

04839 S

i ii i

i 06 i Q2

C6( ) r"——L

>-« 0>

)

1 11 1 11

' ,lh

1 Q5 * Q3 l 07

f^^C ) C

) ( ^ -T

1 Q4

x

h

—1—

i

)

R2 c1

J21

Rl -

Ql

Figure 5-7. Triac PC Board


Rev. 11/90 5-19/5-20

Son~all® Instruments ~~~~~~~~~~ 2 Service

~~~~~ ~~~ ~~~~~\~~ss'~.~~~~~~~9

~~~ ~~

~~ \ A ~~20 ~ ~~~~ ~~~ ~~~~~~

————— ~ ~~~~ ~~ ~ ~ ~ ~ ~ \ ~~ ~ ~~ ~~

3600 ~~~ ~~——~~

~~00 ~~~~ 5~~——

600 ~~~~~ 6~——\

~~~~~ ~ ~——~

~~~~~~ 3

~——~~0~£~~

~~ ~——1

~~ LATCH~~ ~ ~———————— - ~~1

~~

~ +~ \I~~~——

r~~———~

~~———~

~~——————————————————

~~

~

~~~~ ~~0

~~~~

~~~~~~ ~

~~? ~~~ ~

~4~ ~~~

~~~

~~~~~~~~ ~

~

~~5 ~~ ~

~

~7 ~~~ ~

~~1 ~~~ 333~

~~ HOC ~S3~

Z3 HOC 3021

~~4 HOC ~~~l~

~~ ~~~~ ~~~~~

~~~

~oc

3~Si

~7 m~ 303~

~~~ 9 ~——r ~——————~ ———————————~ ~~~~~ ~~~ ~~ ~ ~ ~~~ ~ ~

~?S ~ ~~~~~ ~~ ~~~~~ ~~~~~ ,~~0 ~59 ~Of ~~ ~~~~~—~~ ~ ~~———~~———1————~~~/S ~~~~~

~

~~~~ \~~~s ~~~————————~~~~~——~~~~

~ ~ ~~~~~ ~ ~ ~ ~~~~~ ~3~~ •~

~~~—~~~~—~~———~~——~————~—~~~ 3600~~~ ~~~~

~

~~~~~ ~~~~?/~~ ~ ~~~~~ ~ ~~O ~ ~~~ ~~~ ~~ ~~~

~—~~~~~~~~———~~——~————1—~~~ ~~~~~~~~4

~~~~ ~~ ~~~~~~ ~~~—————————1 ~ \ ~~2~ ~~~

~~~~ ~~o 39 •~~ ~~

°——~~—~~~~————~~———~~~~~~~~4~~~ 600~~~~

~~~~4

~~~ ~~~~ ~?~~~ ~ ~~~~ ~ ~~~~

~ ~~~ 39 •~~~ ~~ ~—~~—i—~~———~~———i————~~~~~~~ ~~~~~

~~~

4

~~~~ ~ ~ ~ \ ~~~ ~~ 5~

6 ~SO 39 •~~ ~

~~~ ~~

~—\ ——~ ~~

~?/4 ~?27 ~~~ ~/~

~ ~~~~ ~ ~~~ ~~~~~ ~ ~~

~~~~

~~

~~~~~~~~~~~ \~,~~

~~~~~~——~ ~~

~~~~~\ ~~ ~~ ~

~~ ~~ ~~ ~\ ~ ~ ~ ~~ ~~ ~ ~ ~~

~ ~~ ~ | ~ ~~ ~~ ~

~ ~~~~ ~~~ I ~~ ~~~ ~~ ~~~| ~~~~~ ~

~~6 7~~6~~E~

~~———~ ~~~————~~ ~ ~~ ~ ~ ~~ ~ ~~~~~

~7

7~~SS~~~~~~~————~~~~ ~~~

~ ~~ ~\ \~ ~~ ~~ ~~ ~~ Y ~~ ~~~| ~~~~

+5~ ~ ~~~

~———\

~~F2 ~~~~~~

1

~~~ 1~

~~~ ~

~01 ~7~ 500V

~ ~e~~~

~ ~~

1 r ~

~ ~

~~~

~1,500V ~~~~~~

~~ ~

~~~ ~ ~~~0~~ ~~~~~~

~ ~h

—~~ ~~~~~

~~,~~~~~ ~~~~~~ ~~ A

~~~~

1~~ ~~~~~1 ~~~

~~~~

I ~ ~~ ~

~1,~~~~ 10,~~~ ~ A ~~~~~

7~S~~~~

—~~ ~~~

~~~ ——~~~—~

~

<~~~~~~

~

~~

~~~

2

3

~~

5

4

F~~

~~~

~~~2~

~~ ~~~~~~~~D~~~

Y

~~?~

~~~~~~——~——————— ~ A~~

~~~~ ~~~ ~~~~~~~~~

~~~

5~ ~~~~~~~~~~~~~~ (7~51-~)

5~~~ (~BI-~)

~J

~~~~9 ~2~5 ~7

~1~~~

~~~~9 ~~~~

~~-5

~~~~~~~~~~~~~ ~~~~~~~~~~~~~ ~~~~~~~~~~~ ~~~ ~~~~ ~~~~5~s~~~~~ ~~~~~ ~~~ ~~~-/S ~ ~~~~~~~±~~~~

(~) ~~~~ ~~~~~~.~~~~~5 ~~~~~~ ~~~~~ ~/~~~~~~~~~~~S

~4

~~~ ~~~~~

~~~ ~~~~r~r6~-~~~~

6 ~~~~~~ -

~~f4 ~~~~ ~

->/~ ~~~ -

Figure 5-8.

Schematic, ~~~~~~~~~~~~~~~ PC Board

~~~ 04537, Revision 4)

5-21/5-22

Rev. 11/90


Table 5-4. Interface PC Board


(Serial Number 8503864 and below)

(key to figure 5-9)

Component


Manufacturer/s

Order No.

J2 Socket, Integrated Circuit/

Dip Solder Tab, 16-pin

Circuit Assembly

Corp.

#CA-185-10SD

J4

Rl, R2, R3,

R4, R5, R6,

R7

R8, R9,

RIO, Rll,

R12, R13,

R14

R16, R18,

R20, R22,

R24, R26,

R28

R15, R17,

R19, R21,

R23, R25,

R27

R29

Ql, Q2, Q3

Q4, Q5, Q6

Q7

CR1, CR4

Connector, PC, Vertical,

Friction Lock, Wafer,

16-position, Male


1/4 W, ±5%, 120 OHM


1/4 W, ±5%, 180 OHM


1/4 W, ±5%, 1k OHM


1/2 W, ±5%, 39 OHM


2 W, ±10%, 47 OHM

Triac, 6A, 400V

Bridge Rectifier, 1.5A

Molex

#09-65-1169

General Electric

#SC141D

Motorola

#MDA942A-5

CR2, CR3 Varistor, 130 VRMS General Electric

#V130LA10A

Zl, Z2, Z3, Integrated Circuit, Triac

Z4, Z5, Z6, Driver, Zero Crossing,

Z7 Opto Coupler

Motorola

#MOC3031

•Indicates standard component available through most suppliers.

5-23


Table 5-4. Interface PC Board




Component


Manufacturer's

Order No.

Cl, C2, C3

C4, C5/ C6

C7

Capacitor, Ceramic Disc/

.01 MFD, 500 WVDC

Sprague

#19C241A2

C8 Capacitor, Electrolytic,


25 WVDC

Illinois

Capacitor

#226RLR025M

C9

TP1, TP2



250 WVDC

Terminal, Swage, 2 Turrett,


Illinois

Capacitor

#226RMR250M

H.H. Smith

#2018C

Figure 5-9. Interface PC Board



5-24

~~~~~~~~ Instruments

~5~~

~~5~~

~~~~~~~~~~ 2 Service

~~~~~ ~~ ~—

~~~~0 ~~~~ ~ ~——

~~~~ ~~~~ 5 ~——

~~~~~~ 2 ~

~~~~~ 3 ~—

~~~~ ~~~ 5—

~~ ~~~~~~~ ~~——————————————————————————————————————————————

~~~~~

~~> +~~ ~/2~——1—————————————

~~~————————————————1

~~~~—————~

~

1

1

~ ~~~~

~ ~

~~~o ~

1~~~

~~~~

~~ I~O ~

\~

~

~s~

~4 ~~~~~O ~~

\~~2

~~~~

~~ ~ ~~~ ~

~~~~~

2

~~~~~~

J

~~~ ~~~~

~ +~

J

~~~ ~~1~

2

~1

HOC~~30~1

~~

~~~~3031

~3 HOC

3031

~?~~~HOC

~~~~

~5

~~~~~303~~

~~

~~~~~3~~~

~7

~~~ 30~1

~~~~~4 ~~1~1

~9 ~~O

~

~~~~~ ~~~

010 ~~~

~~~~

4 ~~~~

~~~~~So

Q~~4 SC/~~

Rl2 ~ ~~~

~~~~~~~ ~

~~~~ ~~~4

R13 ~~~

~~~ ~~~~~

R14~ ~ ~~~~ ~

~~1

O~~4 SC~41

~~~~ ~~ R~b ~~~ ~~

~~~ ~7 ~ ~~ ~

~~~~ .0~ ~—————1————~~~——————~~——~—————

~~~~ ~ ~~~~~~~~~ ~~

———————*————~~~~————————

~15~

~e~9 39 ~~~

~~~ .01

~

~~~ ~~~~

~~~~~~ ~

~~~ ~ 3~ - ~ •°~

~~

~ ~~

—————*———~~~——————

~i5~~ ~~3

f ~9 ~~~~~~ ~~~

~~

~~ ~~~

~~~~5~~1 ~~~~~T 39 ~~~

~~~~ ~~~~

~ ~~~ ~~~~~~~~ ~~

~~~ ~

1 3~ C7 ~~~ .01

~ ~~

-

~~~~

~—~~———~———— ~~ ~~~ ~~~ ~' ~

~47~~~~~~ ~~~~~~~~ ~9~ 2~~

~~~

~~ ~—~~~~~~—————

~~

~~ ~~~~~~~~ ~~~~~~~~

~~~~~ ~ ~~~

~~~

~ TP2

1

+lc~~~22

-~

~~~~~1

+1 ~~8 T~ ~~,25V

——~~~ ~~~~~~

—>~2 ~~~~

—~7 D~~~A~~7~ ~~

~——?~9 ~~~~ (T~~

—~ & D~CA~T —

1—~'3 ~~~~~ —

——~~ ~~~~

~~/6 I~~~ V(~B 1-2~~

—~~ ~5~~ ~~~~~~~~~~

~~.~~~~ss ~~~~~~~~~~ ~~~~~~~~~~~ ~~ ~~ ~~~~~~~~~ ~~~£ ~ ~~~~~ ~~~~~~~~~

~~ ~~~ ~~~~~~~~~~~~ ~~~ ~~~~ ~~~~~~~~~~~~

~~~~~~

~~4 ~~~~ ~~~~~~~

Figure 5-10.

Schematic, Interface PC Board

~~~ 04537 Revision 1)


5-25/5-26


g. Automatic Wash Cycle

When power is applied, the software lights the AUTO, LID and STOP

indicators and displays zeroes on the displays. The drive motor is

placed in the brake mode (a power outage safety precaution) for five

seconds, and all the panel switches except lid are locked out for this

time period. The automatic wash sequence is described in table 5-5 and

illustrated in figure 5-11. Pressing a particular switch in the cycle

column results in particular values showing up in the digital display

as follows:

Cycle Cycle Display Time Display

080

160

240

320

h. Stepping

Each depression of the step switch results in the software avoiding

a particular sequence of the auto cycle as shown below:

Number of Step Automatic Wash Time

Depressions Segments (Seconds)

321 ^Fill 09

I 9 sec._______^^

^ Spin 55

9+55 sec._______^^

^Decant 10

9+55+10 sec._______^

Agitate 06

Since the full step takes 9 seconds, when this step is skipped the

software deducts 9 seconds from the total time displayed. Pressing the

step switch twice results in 9 plus 55 seconds (total of 64) being

deducted, since the spin portion of the cycle takes 55 seconds. An

additional ten seconds are deducted for the decant portion of the cycle

when the step switch is pressed a third time. The software ignores any

further depressions after the third, since the user might as well begin

a new wash cycle by selecting a new cycle number in the cycle column,

pressing STOP in the command column or selecting AUTO in the mode

column.

i. Checking

The checking feature may only be used during the automatic wash cycle.

Once CHECK is pressed, the software stops at the next convenient point:

after fill, after high speed spin, or after the decant segment. Timing

stops and resumes when the run is started again.

5-27


Lack of saline flow automatically results in a check stop after the

fill segment, an intermittent beeping signal and illumination of the

word saline on the panel. At this point the software allows the user

several options as follows:

1. Do nothing about the lack of saline and continue the run; the

audio warning stops but SALINE remains lit.

2. Prime the system before continuing the run; both the audio

warning and the saline light are turned off.

3 . Disable the audio warning while deciding which option to pursue.

This is done by pressing PRIME* with the lid closed (priming

will not take place unless the lid is open).

j. Alarm

The alarm feature allows the user to request an audible signal when

the instrument times out from an automatic wash cycle. The request may

be cancelled at any time by pressing the ALARM OFF switch. Once the

alarm sounds (a continuous beeping) it can be stopped by pressing ALARM

OFF or STOP.

k. High or Low Spin

Selection of the high or low switch constitutes a manual run. The user

inputs desired run time by using the white portion of the keyboard.

The time is displayed instantaneously, with the latest number selected

appearing on the right of the display after moving previous digits to

the left (the same as a calculator display) . To select a run exceeding

999 seconds, the user presses HOLD. This allows for continuous run which

must be stopped by the operator at the desired time. The time display

will read 0 when HOLD is selected.

NOTE

A five second agitation segment always proceeds a run in

either the high or low mode of operation.

1. Agitate

A five second agitation segment comes at the end of every automatic

wash cycle. Each depression of the agitate switch yields an additional

five seconds of agitation.

•With the exception of STOP, CHECK commands are acknowledged only when the instrument is at rest as indicated by illumination of the

lid light.

5-28


m. Prime

The system will prime for six seconds each time PRIME is pressed if

the following conditions are met: the instrument is at rest/ the system

is in the AUTO mode/ cycle 2/ and the lid is open. In a saline alarm

situation/ the only condition necessary before selecting PRIME is an

open lid.

n. Stopping

Stop is treated as an emergency situation. The software stops all

operation immediately and prepares the system for a complete restart.

Originally programmed run time is retrieved from memory and displayed.

o. Starting

The software acknowledges the start command under only four situations

as follows:

1. Starting in AUTO mode with the instrument at rest.

2. Starting in AUTO mode with the instrument at CHECK.

3. Starting in manual mode (high or low) with the instrument at

rest.

4. Starting the instrument after stopping as the result of a no

latch situation (latch must now be in place.

In all four of these situations the instrument will not start unless

the following prerequisites are met: the lid is latched/ HOLD or a time

other than 0 has been selected/ the instrument is at rest (lid light

illuminated/ and neither priming nor agitation is in progress.

5-29

Table 5-5. Automatic Wash Sequence

(key to figure 5-11)

Actions Time (Seconds)

Start 1

1200* rpm (low) ON 1

Pump ON 6

1200* rpm and pump OFF 1

3600** rpm ON 47

3600** rpm OFF 1

Brake ON 5

Brake OFF 2

Decant coil ON 1

600 rpm ON 4

600 rpm OFF 1

Brake ON 2

Brake OFF 1

Decant coil OFF 1

Agitate 5

Delay 1

TOTAL TIME/CYCLE ......... 80

4000 i

3500-

3000-

2500-

SPEED (RPM)

2000.

1500-

1000-

500-

(

•

3 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80

—

60Hz

50Hz

FILL r----r '..-.'t

1

i

\ DECANT AGITATE

r\

1 I \ AAAA

HIGH SPEED SPIN

...———.\

\ BRAKING

-<- 50Hz 1

— enu7 1

START TIME (SECONDS)

STOP

Figure 5-11. Cellwasher 2 Automatic Wash Sequence

*1500 rpm for 50 Hz

"3000 rpm for 50 Hz

5-30


5-2. Touch Switch Panel

This PC board consists of seven LED indicators and twenty-five normally

open switches. Twenty-four of these switches are arranged in a 6 by

4 matrix. A +5 VDC supply is required for the indicators. Refer to figure

5-13, Touch Switch Panel Schematic.

a. Indicators:

By applying +5 VDC at pin 20 of P5/ any of the indicators (AUTO, HIGH/

LOW, CHECK, START, STOP or LID) could be lit by providing a current

path via a 390 ohm resistor to ground (see figure 5-12).

Figure 5-12. Indicator Current Path

b. Switches:

Grounding any of the columns (MODE, CYCLE, TIME I/ TIME 2, TIME or

COMMAND) simulates the scanning of that switch column. Pressing any

switch from that column results in a low voltage level (ground)

appearing on the corresponding data line. A, B, C or D (see table 5-

6). Pull-ups to +5 VDC are required on all four data lines. Pressing

the lid switch only transfers the logic level from lid switch 1 to lid

switch 2.

5-3. Interface PC Board

This board interfaces the low voltage level of the microcomputer with

the high voltage components in the instrument. The board requires +5

VDC and 115 VAC to operate. Figure 5-14 shows the test set-up for the

following circuit functions on the interface PC board: pump, 3600* rpm,

1200** rpm, 600 rpm, and brake. The test set-up for the remaining two

circuit functions, decant and open, is shown in figure 5-15. The

expected response of each of the seven segments of the interface PC

board are described beginning on page 5-35.

*3000 rpm for 50 Hz

*1500 rpm for 50 Hz

5-31/5-32


~~ ~0

~~~~ 9

~~~ ~~ ~

~ ~~ ~

CH~CK ~3

~~~OD~~~ 5 ~~

5C~ ~~~~~

~~~27~ ~~ ~~

LI~ 51 ~9

~~LID ~~~ ~~3 ~

~~~ ~~~~/~~~~ ~

~~SC4~~7~~~2)

~~~~~~~~~~~~~~~ 6

~~~~~~~~~~~~ ~

~~~~~~ &

~~~

~ ~5~

~ ~

1

~ ~

1

1

1 ~~~1

~ ~

~ 1

1

~ S2

~~~ I

~ 1

~

~~~~

~

\~

~~

~

~~~~————————————

~~ ~

~~

~ ~

~

~

~~~~~

~~~

~ ~~~1

\ ~~~

\

~

~OD~

~;

———~~~~~

~~~~~ ~

~~~~l ~—~ ~

~~~~~———

~~

~~

~~

~—0 0 1

~~~ 1

~~ ~

• ~

| CHECK

~~~~~~

~

~

5~

1

~

56 | ~

~ .

~~57

~~

1

1

58 1 ~

~~YC~E

1

~~~

2

~~~ ~

3

~~0 O~

4

~

~~ ~~~

~~

~

LID

[~0 ~~~~S~

~————————————————————————————~~——————~———

~~~~

\

\

1

1

~

~

~—0 0 5Y~ 5/~

4

~~~ O 5~~ 5/5 ~

7

~~0 0 5~2 ~~/6 ~

~~~~~

~L~RM

~~~~~~~

0 5~3 3/7 ~

————~~——————————

~

[—0 0 5/~

5

~~0 0 5/9 ~

0

~~0 0 S20 ~

~

~~~ 0 ~21 ~

~~~1——————————

1 ~~

~ 1

~~ ~

-

~ ~

~~~ 0 1

5~~~————I—————————

9

~~~~0 0

1 524

C~R~ 1

~~~~~~~ ~

~~~ 9 ~

~~~~~- -1~~

CO~MAND

~~ ~6~~~~~

1

1

1

~

~~ ~

~7~~~?~ I

~~~~ ~

5Z2

~~~~~~~~~

® |

STO~ I

~ 1

PRIM£

~~0 0 |

~~~~7E

~~~

P~~~~~~~

~ ~

l~

~

1

1

1

———————————~~1

1

~~

~

14~ A

~ ~~ B

~~~~

~ 17 ~

Figure 5-13.

Schematic, Touch Switch Panel

5-33/5-34

a. Pump (refer to figure 5-14)

Connect load and meter (AC) across pin 9 and pin 14 of J4 . Apply

power to the board. Closing Sl at pin 9 of J2 results in a 115 VAC

± 5% reading across the load. Opening Sl results in a .1 ±.5 VAC

reading across the load.

b. 3600 rpm (3000 rpm for 50 Hz/ refer to figure 5-14)

Connect load and meter (AC) across pin 8 and pin 9 of J4. Apply

power to the board. Closing Sl at pin 1'or J2 results in a 115 VAC

± 5% reading across the load. Opening Sl results in .1 ±.5 VAC


c. 1200 rpm (1500 rpm for 50 Hz/ refer to figure 5-14)



± 5% reading across the load. Opening Sl results in .1 ±.5 VAC


d. 600 rpm (refer to figure 5-14)





POWER SUPPLY

Q Z 0

<1

u 0 > in +

•>/<1

1-

LU z

1)/ <•

u <

in ^

•>/<' !>/

INTERFACE PC BOARD

J4

< <•

-^HI———L<9<-

DUMMY LOAD

200 n, 200W

AC VOLTMETER

Figure 5-14. Interface PC Board, Test Set-up #1

5-35

e. Brake (refer to figure 5-14)





f. Decant (refer to figure 5-15)

Connect load and meter (DC) across pin 6 and pin 7 of J4. Apply

power to the board. Closing Sl at pin 3 of J2 results in a 115 VDC

± 5% reading across the load. Opening Sl results in a .1 ±.5 VDC


g. Open (refer to figure 5-15)

Connect load and meter (DC) across pin 13 and pin 14 of J4. Apply

power to the board. Closing Sl at pin 16 of J2 results in a 105 VDC

± 5% reading across the load.

Flow, Sl latched and S2 latched are low level signals from the

instrument's flow detector and latching mechanism, respectively.

The destination of these signals is the microcomputer board.

POWER SUPPLY

0

0

c

u Q

in +

•>/<1

h. 3 UJ z

!>/ <f

u

> in

!>/<! !>/

INTERFACE PC BOARD

J4

-^ <r

< <-

DUMMY LOAD

200 ft, 200W

DC VOLTMETER

Figure 5-15. Interface PC Board, Test Set-up #2

5-36


5-4. Microcomputer Board

This Board contains a single chip microcomputer (Z4) with resident 2048

bytes of software and a couple of interface circuitries. The

microcomputer chip has four 8 bit input/output ports: 0, I/ 4, 5. The

microcomputer board responds to certain inputs by controlling a set

of outputs.

a. Hardware

Taken as a black box, the input/output configuration of the

microcomputer PC board is as shown in figure 5-16. Input of data from

the touch switch panel is multiplexed. Output II scans all the columns

of switches. A, B, C/ and D inputs could have come from any of the six

columns. The microcomputer scans the touch switch panel at 10

millisecond intervals (see figure 5-17) by placing a low at each of

the scan lines: MODE, CYCLE/ TIME 1, TIME 2, TIME 3 and COMMAND. Pressing

a switch in a column that is being scanned results in the transfer of

a low to the corresponding data line.

Table 5-6 is a mapping representation of the scanning process. For AUTO

to be registered in the microcomputer, the mode column must currently

be under scan (i.e./ the mode line low) and the auto switch must be

pressed. Data on the A, B, C and D data line should be 0 1 1 1. All

the scan lines can be checked in conjunction with the A, B/ C and D

data lines using the touch switch panel (or a simulation) and the

information in Table 5-6. A new input/output format can be constructed

for the microcomputer as shown in figure 5-18.

Input I signals are logic levels from the instrument into the

microcomputer/ while Input II signals are from the user via the touch

switch panel (refer to figure 5-18).

Output I signals are from the microcomputer PC board to the touch switch

panel status indicators. Output II signals are output to control the

instrument components via the interface PC board. In Output III,

"enable" is used to enable or disable the A, B, C, D data tristate buffer

(Z8) . "Beep" activates the beeper. Output IV carries data to the seven

segment LED displays, while Output V selects which of these displays

gets that data.

The refresh rate is once every ten milliseconds (see figure 5-18) for

each of the four displays.

1. Input I:

The first input from the instrument system is the saline flow

signal. During the filling period of the automatic wash, a low

on the flow line is taken by the microcomputer as a no-saline

situation. This signal is the output of the saline detector

switch via the interface board.

5-37


INPUT/OUTPUT

INPUTS OUTPUTS

FLOW v

Sl LATCHED v

S2 LATCHED \ ————————————7

^—————^

B Y

r

)

-B—I

0 rpm for 50 Hz

0 rpm for 50 Hz

MICROCOMPUTER

BOARD

4-1 g — a L-

( SEGMENT

( DIGIT SE

START .

STOP Y

AUTO .

^———>

^———>

CHECK .

LID .

ALARM ^

SALINE .

MODE .

CYCLE .

TIME 1 Y

TIME 2 .

TIME 3 .

COMMAND .

DECANT Y

BRAKE .

600 RPM .

1200 RPM* .

3600 RPM** .

PUMP .

OPEN .

ENABLE .

BEEP s

II

III

IV

Figure 5-16. Microcomputer Board Input/Output

Configuration

5-38


-10

bx2)®®®®®®®®® te © ® @ © @ ®

® ® 0 ® ® © ® (D ,® (3) (4) ®

123456 7 0 9 10 11 12 13 14 15 16 17

TIME IN MILLISECONDS

KEY

NUMBER / ACTIVITY PERFORMED

1

2

3

4

5

HOUSEKEEPING

MONITOR

DISPLAY

SCAN

CONTROL

RTN

RTN

RTN

RTN

Figure 5-17. Microcomputer Scan Routine

5-39


Table 5-5. Microcomputer Scan Map

SWITCH

PRESSED COLUMN SCANNED DATA

MODE CYCLE TIME 1 TIME 2 TIME 3 COMM A B C D

5-40


INPUTS

INPUT/OUTPUT

FLOW

Sl LATCHED

S2 LATCHED

AUTO

HI

LO

CHECK

CYCLE 1

CYCLE 2

CYCLE 3

CYCLE 4

1

2

3

4

5

6

7

8

9

HOLD/ALARM ON

0/STEP

CLEAR/ALARM OFF

START

STOP

PRIME

AGITATE

MICROCOMPUTER

BOARD

START INDICATOR

STOP INDICATOR

AUTO INDICATOR

HI INDICATOR

LO INDICATOR

CHECK INDICATOR

LID INDICATOR

ALARM INDICATOR

SALINE INDICATOR

DECANT

BRAKE

600 RPM

1200 RPM (1500 rpm-50 Hz)

3600 RPM (3000 rpm-50 Hz)

PUMP

OPEN

ENABLE

BEEP

SEGMENT a )

SELECTOR 9 )

DIGIT Dl )

SELECTOR D4 )

III

IV

Figure 5-18. Creating New Input/Output Formats

5-41


2. Sl Latched and S2 Latched:

To ensure proper latching to the instrument lid, these two lines

ought to be low. These signals are outputs from the latching

mechanism via the interface PC board.

3. Input II:

All the inputs from the user enter via the touch switch panel

PC board. Table 5-6 contains the expected responses of the

microcomputer PC board to these inputs in conjunction with the

other circuitries on the board.

4. Output I (User Indicators):

To illuminate the corresponding touch panel indicator(s)/ the

start, stop, auto, high, low, check or lid line(s) would go low.

The alarm and saline lamps resident on the microcomputer PC

board illuminates when a line goes low.

5. Output II (Instrument Control):

A low on the decant, brake, 600 rpm, 1200 rpm, pump or open line

activates the triac driver circuitries in the interface PC

board. This triac drives then switches on the corresponding

instrument components.

6. Output III:

During the scanning period, enable allows the hex non-inverting

three-state buffer (Z8) to function when data from the touch

switch panel (A, B, C, D) is desired (see figure 5-17). The

second portion of Output III is the beep line. A high to low

level transition on the beep line controls the audible signal

from the instrument.

7. Output IV:

Output IV carries A through G segments data (see figure 5-19)

to the four seven-segment displays. A high on any of these lines

takes power to that particular segment of the digital time

display.

A

", "

•/_/•

;/./1

Figure 5-19. Digital Time Display Segments

5-42


Table 5-7. Electronics Control System

INPUT

(SWITCH PRESSED)

OUTPUT

(RESPONSE)

CONDITIONS

REQUIRED

AUTO

HIGH

LOW

CHECK

CYCLE 1

CYCLE 2

CYCLE 3

CYCLE 4

1 'AUTO' INDICATOR

LINE LOW (INDICATOR

LIT)

2 RELOAD PROGRAMMED

# OF WASH AND TIME

1 'HIGH' INDICATOR

LINE LOW (INDICATOR

LIT)

2 RELOAD PROGRAMMED

HIGH SPIN TIME

1 LOW INDICATOR LINE

LOW (INDICATOR LIT)

2 RELOAD PROGRAMMED

LOW SPIN TIME FOR

DISPLAY

CHECK INDICATOR LINE

LOW (INDICATOR LIT)

DISPLAY 1 080

DISPLAY 2 160

DISPLAY 3 240

DISPLAY 4 320

IN STOP NOT

IN CHECK

IN STOP NOT

IN CHECK

IN STOP NOT

IN CHECK

AUTO MODE

IN START

1 AUTO MODE

2 IN STOP

3 NOT IN CHECK

1 AUTO MODE

2 IN STOP

3 NOT IN CHECK

1 AUTO MODE

2 IN STOP

3 NOT IN CHECK

1 AUTO MODE

2 IN STOP

3 NOT IN CHECK

1 NUMBER DISPLAY

(ON THE RIGHT MOST

DIGIT)

2 PREVIOUS DATA PUSHED

TO THE LEFT.

1 HIGH OR LOW

MODE

2 IN STOP

3 NOT IN CHECK

1 NUMBER DISPLAYED (ON

THE RIGHT MOST DIGIT)


TO THE LEFT

1 HIGH OR LOW

MODE

2 IN STOP

3 NOT IN CHECK

5-43


Table 5-7. Electronics Control System (continued)

INPUT

(SWITCH PRESSED)

OUTPUT

(RESPONSE)

CONDITIONS

REQUIRED

HOLD




TO THE LEFT




TO THE LEFT




TO THE LEFT




TO THE LEFT




TO THE LEFT




TO THE LEFT




TO THE LEFT




TO THE LEFT

1 0 DISPLAYED ON

THE RIGHT MOST DIGIT

2 ALL OTHER DIGITS OFF

1 HIGH OR LOW

MODE

2 IN STOP

3 NOT IN CHECK

1 HIGH OR LOW

MODE

2 IN STOP

3 NOT IN CHECK

1 HIGH OR LOW

MODE

2 IN STOP

3 NOT IN CHECK

1 HIGH OR LOW

MODE

2 IN STOP

3 NOT IN CHECK

1 HIGH OR LOW

MODE

2 IN STOP

3 NOT IN CHECK

1 HIGH OR LOW

MODE

2 IN STOP

3 NOT IN CHECK

1 HIGH OR LOW

MODE

2 IN STOP

3 NOT IN CHECK

1 HIGH OR LOW

MODE

2 IN STOP

3 NOT IN CHECK

1 HIGH OR LOW

MODE

2 IN STOP

3 NOT IN CHECK

5-44



INPUT

(SWITCH PRESSED)

OUTPUT

(RESPONSE)

CONDITIONS

REQUIRED

CLEAR

ALARM ON

STEP

ALARM OFF

START

STOP

PRIME

1 CLEAR DISPLAY DATA

TO ZEROS

1 ALARM LINE LOW

(LAMP ON)

1 SUBTRACTS FROM

DISPLAY

1 ALARM LINE HIGH

(LAMP OFF)

1 START LINE LOW

(INDICATOR ON)

2 START PROGRAMMED RUN

3 LID LINE HIGH

(INDICATOR OFF)

4 CHECK LINE HIGH

(INDICATOR OFF)

1 STOP LINE LOW

(INDICATOR ON)

3 RELOAD ORIGINALLY PRO¬

GRAMMED DISPLAY DATA

2 DISABLE ALL CONTROL

LINES (STOP RUN)

1 PUMP LINE LOW FOR

6 SECONDS

1 HIGH OR LOW

MODE

2 IN STOP

3 NOT IN CHECK

AUTO MODE

AUTO MODE

IN STOP

NOT AGITATING

NOT IN CHECK

NOT PRIMING

AUTO MODE

DOOR LATCHED

PROGRAMMED

TIME NOT ZERO

LID INDICATOR

ON

AUTO MODE

DOOR OPEN

CYCLE 2 SELECTED

IN STOP NOT IN

CHECK OR

IN STOP IN CHECK

AT "NO SALINE"

POINT

NOT AGITATING

LID INDICATOR ON

5-45



INPUT

(SWITCH PRESSED)

OUTPUT

(RESPONSE)

CONDITIONS

REQUIRED

AGITATE

*LID

1 PULSE OF 4 HZ ON

600 RPM LINE

2 PULSE OF 4 HZ ON BRAKE

LINE (PHASE SHIFT OF

170 MS BETWEEN 1 & 2)

1 OPEN LINE LOW

1 IN STOP

2 NOT IN CHECK

3 NOT PRIMING

4 LID INDICATOR ON

LID INDICATOR ON

"Lid switch is not an input to the microcomputer. It is in parallel to the lid indicator which is an output from the microcomputer.

8. Output V:

Dl, D2/ D3 and D4 control display digit selection. A low on any

of these lines selects that particular display.

b. Software

Figures 5-20, 5-21, and 5-22 display all the software activities for

starting in the manual (high or low) mode, check mode, or auto mode

which were not previously discussed under the Hardware section.

5-46


*1500 rpmfor50 Hz

"3000 rpm for 50 Hz

Figure 5-20. Starting in Manual (High or Low) Mode,

Logic Flow Chart

5-47


C START ^

Turn CHECK indicator off.

Continue as in

Auto (no check)

Figure 5-21. Starting from Check Mode,

Logic Flow Chart

5-48

SO

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strum

ents

Cellw

asher

2 S

ervice

N

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3: X

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££

11

8;

Fig

ure

5-22. S

tarting

fro

m

Au

to

Mo

de/

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gic

Flo

w

Ch

art

5-49


5-5. Electronics Module

The instruments' s electronics control is composed of the microcomputer

PC board and the touch switch panel. The electronics control turns on

or off the various components of the instrument. By simulating the

instrument components and instrument status - latched and saline flow

- the electronics control can be thoroughly tested independently of

the instrument. This test is performed using control signals and status

information on the microcomputer board's connector, J3.

a. Requirements:

1. +5 VDC from an external supply or from the instrument's supply

to test points 1 and 2 of the interface board.

2 . Touch switch panel must be plugged into the microcomputer board.

b. Performance:

By using pull-up resistors at the microcomputer control signals

(decant, brake, 600 rpm, 1200* rpm, 3600* rpm, pump or open at J3),

a low on any of these lines reveals which instrument component should

be turned on. Instrument status can be simulated as desired by grounding

or otherwise altering lines: flow, Sl latched and S2 latched.

c. Example:

By pressing PRIME under the correct conditions (Sl and S2 latched high

(door open), AUTO mode/ cycle 2) the pump signal line at pin9 of J3

should be low for five (5) seconds.

* 1500 rpm for 50 Hz

"3000 rpm for 50 Hz

5-50


Section 6. APPLICATIONS

This section describes the use of the Cellwasher 2 in blood testing

procedures. It contains a brief overview of Cellwasher 2 applications,

troubleshooting charts (Table 6-1 through 6-3) for common procedural

errors that may occur during blood testing/ and a glossary of blood

banking terms.

6-1. Cellwasher 2 Application

The Cellwasher 2 is used in routine blood work to speed up and simplify

the procedures for determining human blood groups and types. Often/

the results of blood tests made using the Cellwasher 2 determine donor/

patient compatibility for blood transfusions/ and the results of these

tests must be accurate to prevent serious transfusion reactions.

Although the Cellwasher 2 performs accurate washing, decanting and

centrifugation of samples/ it does not eliminate all potential errors.

If testing procedures provided by the reagent manufacturers are not

followed exactly or if test materials are outdated, accurate test

results cannot be guaranteed. Proper blood bank procedures require

adequate controls of all tests performed with this system.

6-2. Compatibility Testing

In compatibility testing/ blood samples taken from a donor and patient

are crossmatched to ensure that the patient will be able to accept the

donor's blood. An accurate compatibility test is mandatory, since a

transfusion reaction caused by incompatible blood may be fatal. The

crossmatch is a test tube procedure which will show the technician what

will happen if a donor's blood is transfused to a particular patient.

A transfusion reaction occurs when the patient's immune system treats

the transfused blood like a bacterial infection; that is, the

antibodies in the patient's blood plasma agglutinate (bind together)

the donor's red blood cells in an effort to eliminate them from the

bloodstream. Massive agglutination results in restricted blood flow,

which may in turn cause permanent organ damage or death. In addition/

destruction of donor cells releases agents into the bloodstream in

intolerable amounts.

To determine donor/patient compatibility/ the antibodies in the

patient's blood are detected using a laboratory procedure that

simulates conditions within the patient's blood stream during

transfusion. Three tests for compatibility are performed using the

Cellwasher 2.

6-1


1. Test for direct-reacting antibodies:

Direct-reacting antibodies agglutinate red blood cells readily.

For this test, a suspension of red blood cells in saline is combined

with human blood plasma in test tubes and spun in the Cellwasher

2. When centrifuged, the antibodies and red cells are brought close

enough together to allow agglutination to occur. The test tubes

are removed from the Cellwasher 2, and results are read over a light

source. Agglutination indicates the presence of antibodies in the

plasma against antigens on the red cell surface.

2. Test for warm-reacting antibodies:

Warm-reacting antibodies will agglutinate red cells only after

incubation at 37 C. In this test, the red cells are suspended in

albumin, then combined with human plasma. The test tubes are then

spun again and read to determine test results.

3. Test for Coomb's reacting antibodies:

Coomb's reacting antibodies will agglutinate red cells only from

which all unattached protein have been removed. In the Coomb's

test, anti-human serum (one which contains antibodies derived from

animal) is added to red cells that have been washed to remove

unattached human protein. This combination is then centrifuged to

determine test results.

6-3. Cellwasher 2 Washing Cycle

For the Coomb's reaction to occur, the suspension of red blood cells

must first go through the complete washing cycle. The washing cycle

removes unattached protein from the red cell suspension through a

series of dilutions with saline.

During the washing cycle, plasma and red blood cells are separated by

centrifugation. The saline is pumped into the test tubes automatically

and the tubes are centrifuged again. The saline is then decanted

automatically by centrifugation, with the test tubes at a -5 angle.

The washing cycle can be repeated automatically up to four times by

setting the number of washes on the wash cycle selector.

6-2


Table 6-1. APPLICATIONS TROUBLESHOOTING

CHART: FALSE NEGATIVE RESULTS

PROBLEM

Modify the cell suspension concentration.

Repeat the test, noting antiglobulin step.

Repeat the test with fresh reagent.

Repeat the test with fresh reagent.

1. Check the incubation time.

2. Check the volume of reagent added.

Increase spin time.

6-3



CHART: FALSE POSITIVE RESULTS

PROBLEM

1. Change the saline supply.

2. Clean and decontaminate the tubing and

the distributor.

Change the tubing if necessary.

Repeat the test with fresh reagent cells.

Crossmatch versus fresh reagent.

Test the patient serum for bacterial

contamination.

1. Test versus fresh reagent.

2. Observe patient red blood cells

microscopically..

Decrease spin time.

6-4



CHART: INCONSISTENT REACTION STRENGTH

PROBLEM

1. Change the saline supply.

2. Clean and decontaminate the tubing and

the distributor.

Change the tubing if necessary.

Repeat the test with fresh reagent cells.

Crossmateh versus fresh reagent.

6-5


6-4. Glossary of Blood Banking Terms

The following glossary contains common terms used in blood banking

procedures. For more in-depth applications information, refer to the

Technical Manual of the American Association of Blood Banks.

Agglutination:

Agglutin:

Agglutinogen:

Antibody:

Antigen:

Antigenic Determinant:

Antigenicity:

Antiglobulin:

Autoagglutinin:

Autoantibody:

Avidity of an

Antiserum:

Blocking Antibody:

6-6

The clumping of red blood cells by ag-

glutins.

An antibody that causes clumping of red

cells in a saline medium by reacting with a

specific antigenic determinant.

An antigen that stimulates the production of

agglutinins.

A substance that appears in plasma or body

fluids as a result of antigenic stimulation

and reacts specifically with that antigen in

some observable way.

A substance that, when introduced into a

person lacking this substance, stimulates

production of an antibody.

The portion of an antigen that reacts in a

specific, demonstrable way with an anti¬

body.

The ability of an antigen to stimulate

antibody production.

An antibody produced in an animal in

response to the injection of human globulin;

also called anti-human globulin.

An agglutinin that reacts with the red blood

cells of the person in whose serum it is

found; it usually agglutinates the red blood

cells of most other persons also.

An antibody that reacts with the red blood

cells of the person in whose serum it is

found; it usually reacts with the red blood

cells of most other persons also; see

autoagglutinin.

The strength of reaction between an anti

serum and the corresponding red blood cells .

An incomplete antibody that coats the

receptor sites of red blood cells and

prevents subsequent reaction with a saline

agglutinin directed against the antigenic

determinant.


Blood Group:

Chromosome:

Cold Agglutinin:

Complement:

"Complete" Antibody:

Coombs Test:

Crossmatch:

Enzyme-treated Cells:

Globulin:

Hemolysins:

Hemolytic Disease

of the Newborn:

Hemolytic Transfusion

Reaction:

Hemolyzed:

Immunization:

Incomplete Antibody:

Classification of blood according to anti¬

gens possessed by the red blood cells.

One of a number of rod-shaped dark staining

bodies situated in the nucleus of a cell/

only distinguishable at the time of cell

division. Genes are presumed to exist in

linear arrangement on the chromosomes.

An agglutinin reacting best in a cold

environment (for example, 4 C) with progres¬

sively decreasing activity at higher tem¬

peratures; usually nonreactive at 37 C.

A complex of substances in fresh normal

serum, causing lysis of red blood cells

sensitized by hemolysins.

An antibody that agglutinates red blood

cells in saline solution.

A test of red blood cells by antiglobulin

technic.

An in vitro test of serologic compatibility

between a donor' s blood and that of a

prospective recipient.

Red blood cells treated with a proteolytic

enzyme to be used for antibody identifica¬

tion or demonstration.

A variety of protein found in serum.

Antibodies are globulins.

Antibodies that activate the complement

system, leading to lysis of red blood cells

with the corresponding antigens.

(Erythroblastosis fetalis) A disease caused

by a maternal blood group antibody attacking

fetal red blood cells.

A reaction associated with destruction of

red blood cells in vivo following transfu¬

sion, usually due to serologic incompati¬

bility between donor and recipient.

Disrupted cells.

The process by which an antibody is produced

in response to an antigenic stimulus.

An antibody that adheres to the surface of

red blood cells suspended in saline but

fails to agglutinate them.

6-7


Iln Vitro:

In Vivo:

Irregular Antibody:

Isoagglutinin:

Isotonic Sodium

Chloride (Saline)

Laked/Lysis:

Panel of Cells:

Reagent Red Blood

Antibody Cells

(Human):

Sensitization of

Red Blood Cells:

Specificity:

Titer:

Titrate:

Warm Antibody:

Wash:

6-8

Pertaining to a process or reaction occur¬

ring outside of a living organism, usually

under laboratory conditions (for example,

in a test tube).

Pertaining to a process or reaction occur¬

ring in a living organism.

An antibody not regularly present in the

plasma, e.g., anti-RH (d) in Rh-negative

person.

An agglutinin directed against an antigenic

determinant found in an individual of the

same species.

0 . 85%-0 . 9% aqueous solution of sodium chlor¬

ide (8.5 gram Nad/liter = 0.85% normal

saline).

The disruption of cells.

A set of reagent red blood cells of known

antigenic content, specially selected and

used for the identification of blood group

antibodies.

Red blood cells used in laboratory testing

ing, e.g., in antibody detection and ident¬

ification of blood group antibodies.

Red blood cells are said to be sensitized

when they have antibody specifically at¬

tached to their receptors but are not

agglutinated or lysed.

The capacity of an antibody to react only

with certain chemical groupings possessed

by the antigen that stimulated its formation

and sometimes possessed by other substances

as well.

The end point of a titration.

To determine the strength of an antigenic

determinant or an antibody by making a

series of dilutions and testing their

strength against a corresponding antibody

or antigenic determinant of known strength.

An antibody reacting at 37 C more readily

than at lower temperatures.

Successive centrifugation of cells in large

volumes of saline to free them from the

liquid in which they were originally sus¬

pended (usually serum or plasma).


Section 7. TROUBLESHOOTING

This section contains troubleshooting charts to locate the probable

cause of mechanical and electrical problems that may occur in the

Cellwasher 2. Refer to Section 5 for schematics and wiring diagrams

as needed. If repair or replacement is required, refer to section 8.

WARNING

Because tampering with high voltage electrical circuits

can cause severe electrical shock/ untrained personnel


in this instrument.

If the instrument has been used with radioactive or

biohazardous samples, it may be contaminated. The

instrument and rotor must be certified free of biological

or radioactive contamination: a Decontamination Informa¬

tion Certificate must be filled out by the user.

Appropriate precautions and decontamination procedures

must be followed when servicing the instrument. DO NOT

service or repair any equipment for which the proper

decontamination procedures have not been performed.

Rev. 11/90 7-1

Cellwasher 2 Service SORVALI® Instruments

Table 7-1. Troubleshooting Chart: Drive Motor

PROBLEM

7-2 Rev. 11/90


Table 7-2. Troubleshooting Chart: Power

PROBLEM

Cellwasher inoperative and displays not illuminated. )

Rev. 11/90 7-3

Cellwasher 2 Service SORVALI® Instruments

Table 7-3. Troubleshooting Chart: Saline Fill

PROBLEM

^ Improper saline fill of tubes.

7-4

V e S w( Replace gasket.

Rev. 11/90


Table 7-4. Troubleshooting Chart: Decant

PROBLEM

'After automatic decant, the volume of saline solution left in a tube test may appear to be more than the amount left after manual decanting. This is NOT a decant malfunction. The automatically decanted tube appears to retain more fluid because the saline solution remaining in the tube has been centrifuged to the bottom, while a manually decanted tube retains fluid on the entire inside surface of the test tube. To obtain an accurate comparison, centrifuge the hand-decanted tube for about fifteen seconds. This will force all retained liquid to the bottom of the tube for a direct visual comparison of the two decanting methods.

Rev. 11/90 7-5

Emil Heerman

Yes

Emil Heerman

No

Emil Heerman

Emil Heerman

Ccllwasher 2 Service SORVALI® Instruments

Table 7-5. Troubleshooting Chart: Lid Latch

PROBLEM

Check magnet in lid. Replace

latch assembly.

Replace micro computer board.

7-6 Rev. 11/90


\ DECANT COIL

Figure 7-1. Location of Decant Coil and Drive Ring

Figure 7-2. Location of Lift Plate Ring

Rev. 4/96 7-7/7-8


Section 8. REPAIR and REPLACEMENT PROCEDURES

This section contains repair and replacement procedures for the

Cellwasher 2. Should service beyond the scope of this manual be

necessary, contact your DuPont Service Representative. Be sure the

user has performed appropriate decontamination procedures/ as

outlined in paragraph 1-3, before attempting any repair or replacement

procedures on the instrument.

WARNING

Because tampering with high voltage electrical cir¬

cuits can cause severe electrical shock/ untrained personnel


in this instrument. Unplug the Cell-washer 2 before

proceeding.

8-1. Base Plate Removal

a. To remove the base plate:

1. Unplug the instrument power cord.

2. Remove the distributor, rotor and stainless steel rotating

bowl assembly from inside the cellwasher chamber.

3. Turn the instrument on its side.

4. Remove the four (4) mounting screws and rubber feet.

5. Remove the base plate.

b. To replace the base plate, reverse the above procedure.

8-2. Cabinet Removal

a. To remove the cabinet:

1. Switch the power ON and press the LID button to release the

lid.

2. Switch the power OFF and unplug the instrument power cord.

3. Disconnect the tubing from the saline flow detector (the

tubing that connects the detector to the dispenser in the lid) .

4. Remove the wet guard.

5. Remove the four (4) 1/4-20 x 1-3/4 inch long socket head screws

that secure the cabinet to the chassis using a 3/16 inch Alien

wrench.

8-1


6. Lift the cabinet up approximately four (4) inches to

disconnect the lid latch cable connector (squeeze the two

locking tabs on the connector) located to the left rear corner

of the instrument.

7. Lift the cabinet off of the chassis.

b. To replace the cabinet, reverse the procedure given above and

on the previous page.

8-3. Front Panel Replacement

a. To remove the front panel:

1. Remove the cabinet (paragraph 8-2).

2 . From behind the front panel, remove the four (4) mounting nuts

and washers.

3. Remove the front panel/front wet guard from the chassis.

4. Disconnect the harness connector located at the back of the

front wet guard.

5. Remove the electrical tape at the top edge of the front panel/

front wet guard. Separate the front panel from the front wet

guard, then disconnect connector located inside the front wet

guard.

NOTE

• When reassembling the front panel and front wet

guard, be sure to fold the antistatic electronic

guard (tab), located on the lower right corner of

the front panel, over the edge of the front wet guard

and stud of the front panel.

• To ensure electrical continuity, place a star washer

on the stud which has the antistatic electronic

guard (tab).

b. To replace the front panel, reverse the above procedure.

8-2

SORVALL® Instruments Ccllwashcr 2 Service

8-4. Power Switch Replacement

a . To remove the power switch:

1. Remove the base plate (paragraph 8-1).

2. Remove the two (2) mounting screws from the power switch.

3. Disconnect the connector and remove the switch.

b. To replace the power switch, reverse the above procedure.

8-5. Pump Replacement

a. To remove the pump:

1. Remove the base plate (paragraph 8-1).

2. Remove the four (4) thumb screws from the front of the pump

assembly, and remove the front half of the pump head.

3. Remove the four (4) screws that attach the rear half of the

pump to the frame, and remove the rear half of the pump.

b. To replace the pump, reverse the above procedure.

8-6. Pump Motor Replacement

a. To remove the pump motor:

1. Remove the pump (paragraph 8-5).

2. Remove the rear wet guard.

3. Disconnect the pump motor connector from the harness.

4. Remove the four motor mounting screws.

5. Disconnect the motor ground.

b. To replace the pump motor, reverse the procedure given above and

on the previous page.

8-3


8-7. Flow Detector Replacement

a. To remove the flow detector:


lid.


3. Disconnect the saline tubing from the flow detector.

4. Remove the two screws that secure the flow detector to the

frame.

b. To replace the flow detector, reverse the above procedure.

8-8. Motor Replacement

a. To remove the motor:


2. Remove baseplate (paragraph 8-1).

3. Disconnect the motor connector, the decant coil connector,

and the ground wire.

4. Remove the three (3) 1/4-20 mounting nuts that secure the

motor to the chassis from above. Lift out the motor.

b. To replace the motor, reverse the above procedure.

8-9. Interface Printed Circuit Board Replacement

a. To remove the interface printed circuit (PC) board:


2. Remove the front panel (paragraph 8-3).

3. Carefully disconnect the flex (ribbon) cable between the

interface PC board and microcomputer PC board.

NOTE

Handle the flex (ribbon) cable carefully since it is

easily damaged.

4. Disconnect the connectors on the interface PC board, remove

the four (4) mounting screws and washers that secure the

interface PC board in place. Remove the interface PC board.

b. To replace the interface PC board, reverse the above procedure.

8-4


8-10. Microcomputer Printed Circuit Board Replacement

a. To remove the microcomputer printed circuit (PC) board:


2. Remove the front panel (paragraph 8-3).

3. Carefully disconnect the flex (ribbon) cable between the

interface PC board and the microcomputer PC board.

NOTE

Handle the flex (ribbon) cable carefully since it is -

easily damaged.

4. Disconnect the connectors on the microcomputer board/ remove

the four (4) mounting screws/ then remove the microcomputer

PC board.

NOTE

If the microchip is being replaced see figure 5-3 for correct

orientation.

b. To replace the microcomputer PC board/ reverse the above

procedure.

8-11. Fuse Replacement

a. To remove the fuse in the power supply adapter:

1. Unplug the instrument power cord.

2. Remove the power cord from the power supply adapter.

3. Slide the clear plastic guard to the left of the power cord

connection.

4. Pull out the "FUSE PULL" lever. The end of the fuse will pop

out.

5 . Remove the fuse.

b. To replace the fuse, insert the appropriate slow-blow fuse as

indicated on the rating plate at the back of the instrument.

Move the guard to the right and replace the power cord.

8-5/8-6


Section 9. KIT INSTRUCTIONS

This section includes installation instructions for all available

Cellwasher 2 Cell Washing System service kits.

Rev. n/90 9-1


9-1. Tubing Replacement Kit, PN 12977

A. Parts Supplied

This kit is supplied with three lengths of tubing: one (1) piece of

pump tubing, measuring 10 inches long (PN 04590), one (1) length of

tubing measuring 78 inches long (PN 04876 is 5/16 inch x 7/16 inch)

and one (1) length of tubing measuring 114 inches long (PN 04875 is

1/4 inch x 3/8 inch). The serial numbers given in the table below

indicate which tubing is to be used with your Cellwasher 2.

NOTE The table specifies the proper tubing lengths that are to be cut from tubing, PN 04875

and PN 04876, respectively. Figure 9-1 on the next page shows the parts location.

Item

1

2

3

4

5

6

7

8

9

10

—

—

Description

Overflow Tubing 15 cm (6 inches) long

Vent Tubing 10 cm (4 inches) long

Pump Tubing 25 cm (10 inches) long

Flow Tubing 30 cm (12 inches) long

Discharge Tubing 22 cm (8.5 inches) long

Supply Tubing 60 cm (24 inches) long

Drain Tubing 121 cm (48 inches) long

Pump Connector

Y-Connector

Adjustable Tubing Clamp

Nylon Cable Tie

Installation Instructions

Part Number

SN 9302849

and above

04876(1)

04876(1)

04590(1)

04875(1)

04876(1)

04875(1)

04876(1)

65762(1)

62842 (2)

68671 (2)

65925 (2)

04640(1)

(Quantity)

SN 9302848

and below

04875(1)

04875(1)

04590(1)

04875(1)

04875(1)

04875(1)

04875(1)

65762(1)

62842 (2)

68671 (2)

65925 (2)

04640(1)

B. Tubing Replacement

NOTE If sterilization of the tubing (and connectors) is required, autoclave for 20 minutes at

121°C, then replace tubing as described on the following pages. The tubing (and

connectors) will be very hot once autoclaved and should not be handled until cooled

completely. Also, the tubing can become deformed if handled immediately after it has

been autoclaved.

1. Set the power switch ON and press the LID button to release the

lid.

AWA R N I N G

Because the Cellwasher 2 has high voltage electrical circuits that can cause

severe electrical shock, this procedure must be done by a technician who is familiar with

electrical circuits. To remove the potential of electrical shock, unplug the power cord

before proceeding.

9-2 Rev. 4/96


Figure 9-1. Tubing Diagram: Parts Location

2. Set the power switch OFF and unplug the power cord.

NOTE Retain all hardware for reassembly unless otherwise advised.

3. If the overflow tubing is damaged and must be replaced follow the

procedure given below; otherwise proceed to step 4.

a. Lift the lid and remove the distributor, rotor and rotating

bowl assembly from the inside of the cellwasher chamber.

b.

c.

Lift out the wet guard.

Remove the four (4) 1/4-20 x 1-3/4 inch long socket head screws


wrench.

d. Disconnect the discharge tubing from the outlet port on the

collecting ring and the flow tubing connected to the outlet

port of the flow detector.

NOTE When lifting the cabinet up to disconnect the lid latch cable connector, be careful not to

stress the cable connector.

Rev. 4/96 9-3


e. Lift the cabinet up about four (4) inches. Then, while holding

the cabinet in one hand, reach in through the front panel

opening, to the left rear corner of the instrument and

disconnect the lid latch cable connector by squeezing the two

locking tabs on the connector.

f. Lift the cabinet off the instrument chassis and place it aside

for reassembly.

g. From the back of the motor drain tray remove the overflow

tubing (the tubing may be secured with a nylon cable tie).

h. Cut a length of tubing 15 cm (6 inches) long to be used as

overflow tubing. Then, install the tubing to the motor drain

tray connector. Secure in place using a nylon cable tie

(PN 65925) supplied.

Motor Protection: The overflow tubing allows saline to flow

onto the counter top alerting the operator of a problem with

incorrect saline levels or tube failure.

i. Bring the overflow tubing through the hole in the back of the

cabinet. The tubing will hang free.

j . Place the cabinet over the chassis and reconnect the lid latch

cable connector.

NOTE Make sure the cable connector is properly and securely connected and that the wires

on the chassis are not "pinched" when the cabinet is put in place.

k. Secure the cabinet to the chassis using the four (4) 1/4-20

x 1-3/4 long socket head screws removed in step 3c above.

1. Replace wet guard.

4. Replace the flow tubing connected to the nozzle in the lid as

follows:

a. Open the lid.

b. Remove the four black screws that secure the collecting ring

assembly to the lid.

c. Remove the collecting ring assembly.

d. Disconnect the flow tubing from the nozzle in the lid and from

the flow detector, if not already done. Discard tubing.

e. Cut a length of tubing 30 cm (12 inches) long to be used as

flow tubing. Attach the flow tubing to the outlet port of the

flow detector.

f. Feed the free end of the flow tubing from the flow detector

through the hole in the back of the cabinet, and connect it

to the nozzle in the top of the lid.

9-4 Rev. 4/96


h. Secure the collecting ring assembly in place using the four

black screws.

5. Turn the instrument so that the back of instrument faces you.

6. Remove and discard the supply tubing from the saline supply

reservoir to the flow control valve and from the flow control valve

to the pump connector located on the left side of the peristaltic

pump. Retain the flow control valve for reassembly.

7. Disconnect the pump tubing from the inlet port on the flow

detector. Then, remove the pump tubing from the peristaltic pump

as follows:

a. Remove the four knurled thumbscrews from the peristaltic pump

and remove the front half of the pump housing. Set the pump

housing and hardware aside for reassembly.

b. Remove the pump tubing and connector being careful not to

remove the pump roller assembly. Discard the pump tubing and

the connector.

8. Remove the T-Connector or Y-Connector (with vent tubing/

discharge tubing and drain tubing attached) by pulling up on the

tab corners of the two adjustable tubing clamps.

9. Disconnect the discharge tubing from the outlet port on the

collecting ring, if not already done.

NOTE Before discarding the drain tubing (attached to the bottom leg of a Y-Connector or

T-Connector) remove the connector, if used. Be sure to retain the connector for

installation of new drain tubing.

10. Discard the discharge tubing/ the vent tubing/ the drain tubing

and the T-Connector. Retain the Y-Connector if used.

11. Check the two adjustable tubing clamps for damage and that they

have not become loosened. If the adjustable tubing clamps are

damaged or loose/ replace as follows:

a. Remove and discard damaged or loosened adjustable tubing

clamp.

b. Clean the surface with alcohol or solvent to remove any

residue.

c. Peel the protective sheet from the adhesive back of the

adjustable tubing clamp and approximately position clamp(s)

as shown in figure 9-2.

Rev. 11/93 9-5


19mm

(0.75 in)

Figure 9-2. Adjustable Tubing Clamp Location

12. Install the pump tubing (PN 04590) as follows:

a. Insert the pump connector (PN 65762) into one end of the 25

cm (10 inches) long pump tubing (PN 04590).

b. Turn the roller assembly so that one of the rollers is in the

11 o'clock position.

c. Without turning the rollers, place the pump tubing around the

rollers (the end with the connector should be on the left

side).

d. Pull up on the two ends of pump tubing and fit it into the pump

housing around the rollers.

NOTE Do not try to fit the pump tubing in place with a sharp object, as this could puncture the

tubing.

e. With the pump tubing in place, replace the front half of the

pump housing. Stretch the ends of the pump tubing while fitting

the pump housing in place to eliminate slack in the pump

tubing.

f. Make sure that the halves of the housing are evenly mated. A

gap indicates that the pump tubing is pinched; if so, remove

the front half of the housing and repeat steps c through f.

g. Install the four thumbscrews and hand tighten.

h. Check the pump tubing installation by turning the roller

assembly shaft counterclockwise using pliers (should rotate

smoothly without pinching tubing).

9-6 Rev. 4/96


13. Cut a length of tubing 60 cm (24 inches) long to be used as supply

tubing. Attach the supply tubing to the pump connector on the left

side of the pump housing.

14. If the flow control valve is an in-line type, cut the supply tubing

at a convenient point and insert the flow control valve into the

line. Insert the end of the supply tubing into a saline supply

reservoir (see figure 9-1 to identify which control valve you

have).

If the flow control valve is the pinch valve type/ place the valve

over the supply line at a convenient point and insert the end of

the supply tubing into a saline supply reservoir. The saline flow

rate will need to be readjusted.

15. Connect the pump tubing from the right side of the pump housing

to the inlet port of the flow detector.

16. Cut a length of tubing 22 cm (8.5 inches) long to be used as

discharge tubing. Then, from inside the lid, connect the discharge

tubing to the outlet port on the collecting ring assembly.

17. Bring the discharge tubing through the hole in the back of the

cabinet and connect it to the top right leg of the Y-Connector

(PN 62842) supplied.

18. Cut a length of tubing 10 cm (4 inches) long to be used as vent

tubing. To the top left leg of the Y-Connector install the vent

tubing

19. Cut a length of tubing 121 cm (48 inches) long to be used as drain

tubing. Then, to the bottom leg of the Y-Connector install tubing.

20. Position and secure the Y-Connector (with tubing attached) into

the two adjustable tubing clamps (PN 68671) as described below

(refer to figure 9-3).

a. Open the adjustable tubing clamps by lifting up on the corners

of the outer strap as shown in figure 9-3.

b. Into the adjustable tubing clamp located to the left of the

peristaltic pump, position the bottom leg of the Y-Connector

(with discharge tubing attached).

c. Holding the Y-Connector in place with one hand/ fold the inner

strap over the Y-Connector/discharge tubing. Then, fold the

outer strap over the inner strap and push on the outer strap

to lock in place. You will hear a "click" when the clamp is

secured.

d. Into the adjustable tubing clamp located to the left of the

flow detector, position the vent tubing. (Vent tubing is

installed in the top left leg of the Y-Connector.)

e. Holding the vent tubing in place with one hand, fold the inner

strap over the tubing. Then, fold the outer strap over the

inner strap and push on the outer strap to lock in place. You

will hear a "click" when the clamp is secured.

Rev. 4/96 9-7


PULL UP

ON TAB

CORNERS

TO OPEN

FIRST FOLD

INNER STRAP

OVER TUBING

THEN FOLD

OUTER STRAP

OVER INNER

PRESS DOWN

ON KNURLED

AREA UNTIL

STRAPS "CLICK

TOGETHER

TO CLOSE

Figure 9-3. Adjustable Tubing Clamp

21. Close the lid and check to see that the tubing does not kink

(foldover). If this foldover occurs reposition or rearrange the

tubing on the right leg of the Y-Connector. (Refer to Figure 9-

1/ Item 5).

22. Make sure the drain tubing, connected to the bottom leg of the

Y-Connector, flows downward to an open sink, drain, or waste

receptacle since drainage in the Cellwasher 2 is accomplished by

gravity. Use the shortest length of drain tubing possible and make

sure the tubing is positioned so there is no upward travel to

result in a "trap" for collected waste fluid.

Ideally, the drain tubing should be positioned so that it can not

become immersed in collected waste fluid. However, if there is

a possibility that the drain tubing will become immersed in waste

fluid, cut the drain tubing at a convenient point near the waste

receptacle and insert a Y-Connector (a Y-Connector is supplied

with the Cellwasher 2) . Always insert a Y-Connector in the drain

line when using a waste container for discharged saline.

NOTE The Y-Connector prevents pressure from building up in the line due to submerged

tubing or air blockage. Failure to vent the drain tubing by installing the Y-Connector can

result in improperly decanted samples.

23. Turn instrument so that the front of the instrument faces you and

install rotating bowl assembly, rotor, and distributor into

chamber.

24. Plug in power cord.

9-8 Rev. 4/96


9-2. CoUector Seal Replacement Kit, PN 04353

a. CW-1 Collector Seal Removal/Replacement

The collecting ring assembly consists of a seal pressed between two

rings. The collecting ring, which includes the tab and drain hole/

will be referred to as the cover and the deflector ring as the base

(see figure 9-4).

To remove the collector seal:

1. Remove the collecting ring assembly from the lid by pulling

the tab on the front edge.

2. Separate the cover from the base and remove the old seal.

NOTE

Early instruments may not have a collector seal. On these

instruments, the cover and the base may be glued to¬

gether. Simply pry the two sections apart and remove any

glue from the base and the cover.

Before replacing the collector seal, clean the collecting ring

assembly with warm water and a mild detergent as specified in the

Cellwasher 1 Operating Instruction Manual.

To replace the collector seal:

1. Soak the collector seal in hot tap water for one to two minutes

to soften it.

2. Invert the base so that it rests on the smaller opening and

place it on a table or workbench.

3. Lay the collector seal on the inverted base so that it rests

symmetrically (concentrically) on the upper edge.

4. Invert the cover, then gently place it on the seal and inverted

base so that it also rests symmetrically.

5. Place the thumb of each hand on top of the entire assembly,

slip you fingers beneath the lip of the base, and begin pressing

the two rings together evenly all around the lip.

6. Lift the entire assembly and continue to squeeze together

until both edges are evenly together.

7. Examine the assembly; at no point along the edge should more

than 6 mm (0.25 inch) of the collector seal protrude. If it

does, open the entire assembly and repeat steps 1 through 6.

Once the collector seal is centered correctly, use a sharp

knife or blade to trim the excess flare of material.

Rev. 11/90 9.9


8. Replace the collecting assembly in the lid of the Cellwasher

I/ by placing the drain hole portion of the assembly into the

lid first. Make sure that the tabs on the bottom edge are

snapped in place. Then, with both thumbs, snap in the tabs

on the top of the assembly.

WARNING

If the collecting assembly is not properly installed,

the instrument could be damaged which could result in

personal injury should material exit the chamber. To

check that it is properly installed, carefully examine

the cover to make sure all four tabs are snapped into

place. Then pull firmly along the inside edge of the

collector base; the base should not dislodge.

COLLECTING RING ASSEMBLY (

LID

DEFLECTOR RING (BASE)

COLLECTOR SEAL

COLLECTING RING (COVER)

Figure 9-4. CW-1 Collecting Ring Assembly, Exploded View

b.' Cellwasher 2 Collector Seal Removal/Replacement

The collecting ring assembly of the Cellwasher 2 consists of a seal

pressed between two rings: a collector ring (inslcudes the nozzle for

the discharge tubing) and the retainer ring (see figure 9-5).

9-10 Rev. 11/90


To remove the collector seal:

1. Remove the four mounting screws and washers securing the

assembly to the lid.

2. Pull the assembly away from the lid, and lift the discharge

tubing through the wet guard (which is around the bowl).

3 . Disconnect the saline flow tubing from the spray nozzle in the

lid, pulling it through the hole in the retainer ring.

4 . Separate the assembly by prying off the collector ring and

remove the old seal.

Before replacing the collector seal, clean the collecting ring

assembly with warm water and a mild detergent as specified in the

Cellwasher 2 Operating Instruction Manual.

Figure 9-5. Cellwasher 2 Collecting Ring Assembly;

Exploded View

Rev. 11/90 9-11


To replace the collector seal:

1. Soak the collector seal in hot tap water for one to two minutes

to soften it.

2. Invert the retaining ring so that it rests on the smaller

opening and place it on a table or workbench.

3. Lay the collector seal on the inverted reatian so that it rests

symmetrically (concentrically) on the upper edge.

4. Invert the collector ring, then gently place it on the seal

and inverted retainer so that it also rests symmetrically.

NOTE

Make sure the nozzle for the discharge tubing on the

collector ring will align with the groove in the wet

guard when the assembly is reinstalled into the

instrument.

5. Place the thumb of each hand on top of the entire assembly,

slip your fingers beneath the lip of the retaining ring, and

begin pressing the two rings together evenly all around the

lip.

6. Lift the entire assembly and continue to squeeze together

until both edges are evenly together.

7. Examine the assembly; at no point along the edge should more

than 6 mm (0.25 inch) of the collector seal protrude. If it

does, open the entire assembly and repeat steps 1 through 6.

Once the collector seal is centered correctly, use a sharp

knife or blade to trim the excess flare of material.

8. Reinstall the collecting assembly in the lid of the

Cellwasher 2, by reversing steps 1 through 3 of the removal

procedure.

NOTE

When replacing the four mounting secrews, make sure the

flat side of each washer is facing the knurled

thumbscrew.

9-3. Retainer Ring Replacement Kit, PN 12850

This kit is to be used to replace the old style retainer ring/vinyl

seal configuration in the Cellwasher 2 and Cellwasher 2L Cellwashing

Systems serial number 8702301 and lower.

9-12 Rev. 11/90


a. Parts Supplied

Part Number Description

04538

69722

04867

Retainer Ring

0-ring Seal

Instructions

b. Retainer Ring/Vinyl Seal Removal


lid.

WARNING

Because of the high voltages in the instrument/ unplug

the instrument power cord before beginning this

procedure.


3. If, on the back of your instrument, there are tubing clamps

and a T-Connector: Open the adjustable tubing clamp that

secures the T-Connector in place and remove the discharge

tubing from the T-Connector.

4. Remove the four mounting screws and step washers securing the

collecting ring assembly to the lid.

5. Pull the collecting ring assembly away from the lid, and pull

the discharge tubing up through the wet guard (the molded black

liner) .

6. Disconnect the flow tubing from the spray nozzle in the lid

and pull it through the hole in the retainer ring.

7 . Separate the collecting ring assembly by pulling the collector

ring off. Discard the retainer ring and vinyl collector seal.

c. Retainer Ring/0-ring Seal Installation (Refer to Section 3

figure 3-1)

NOTE

Before installing the retainer ring, 0-ring seal, and

collector ring, clean the collector ring and tubing with

warm water and a mild detergent as explained in Section

3 of this manual.

Rev. 11/90 9-13


1. Place retainer ring (PN 04538 supplied)/ on a table or bench

so that it rests on the smaller opening/ and work the 0-ring

seal (PN 69722) around the outside of the large opening until

it is fully seated in the groove of the retainer ring. Then,

place the collector ring so that it rests concentrically over

the 0-ring seal and press firmly into place.

NOTE

Make sure the nozzle on the collector ring will align with the

hole in the wet guard when the collecting ring assembly is

reinstalled.

2. Insert the flow tubing through the hole in the retainer ring

and connect it to the spray nozzle in the lid.

3. Insert the discharge tubing through the hole in the wet guard,

and position the collecting ring assembly in the lid.

4. Secure the collecting ring assembly in place using the four

mounting screws and step washers removed in step 4, Section

b.

NOTE

When replacing the four mounting screws, make sure the

flat side of each step washer faces the screw head.

5. If the discharge tubing was connected to a T-Connector,

reconnect the tubing to the connector and secure in place with

tubing clamp opened in step 3, Section b.

9-4. Lid Latch Assembly Replacement Kit, PN 12792

a. Parts Supplied

Part Number Description Quantity

04861 Lid Latch Assembly 1

04864 Striker Plate 1

67967 Screw/ Machine/ Hex Socket Flat

Head, UNF, #4-48 x 3/16 inch long 2

• 68756 Screw, Machine, Phillips Pan Head,

Black Oxide Finish, UNF, #4-48 x

3/16 inch long 2

67653 Magnet 1

9.14 Rev. 11/90


a. Parts Supplied (continued)


Cover Plate 1

Warning Label, High Voltage 1

Adhesive, Sealant, Loctite #222 1

Adhesive, Sealant, RTV-103, Silicone

Rubber 1

Screw, Machine, Slotted Flat Head,

SSt, UNC, #4-40 x 3/16 inch long 1

04852

21990

90702

67966

67854

b. Striker Plate Replacement


lid.

WARNING

Because of the high voltages in the instrument, this

procedure must be done by a technician who is familiar

with electrical circuits. To remove the potential of

electrical shock, unplug the instrument power cord

before proceeding.

2. Switch the power OFF and unplug the power cord.

3. Lift the lid and remove the distributor, rotor, and rotating

bowl from inside the cellwasher chamber.

4. Lift out the wet guard.

NOTE

Retain all hardware for installation of the new lid latch

assembly and reassembly of instrument.

5. Remove the four 1/4-20 x 1-3/4 inch long socket head screws


wrench.

6. Disconnect the drain tubing and saline-detector tubing.

Rev. 11/90 9-15


NOTE

When lifting the cabinet up to disconnect the lid latch

cable connector, be careful not to stress the cable

connector.

7 . Lift the cabinet up about four (4) inches. Then, while holding

the cabinet in one hand, reach in through the front control

panel opening, to the left rear corner of the instrument, and

disconnect the lid latch cable connector by squeezing the two

locking tabs on the connector.

8. Lift the cabinet off the instrument chassis and place it

upright on a sturdy table or bench.

NOTE

Check your instrument to see which type of hardware is

used to secure the striker plate to the lid (new

instruments use two flathead screws and older instru¬

ments use two rivets). Refer to figure 9-6 for parts

location and identification.

9. If your instrument has two flat head screws securing the

striker plate to the lid:

a) Remove the two flat head screws using a 1/16 inch Alien

wrench. Remove and discard the screws, striker plate and

magnet.

b) Place the striker plate (PN 04864) supplied, on the inside

of the lid. Apply adhesive (PN 90702) to the two hex socket

flat head screws (PN 67967) supplied, then tighten the

screws to secure the striker plate in place.

10. If your instrument has two rivets securing the striker plate

to the lid:

a) From inside the lid (so that it will not be damaged) drill

into the dimple of each rivet until the head of the rivet

is removed. Remove and discard the remaining portion of

rivet, the striker plate and magnet.

b) From the outside of the lid, use an 1/8 inch drill to

^ enlarge the rivet holes.

c) Place the striker plate (PN 04864) supplied, on the inside

of the lid. Apply adhesive (PN 90702) to the two Phillips

pan head screws (PN 68756) supplied, then tighten the

screws to secure the striker plate in place.

9.15 Rev. 11/90


USING SLOT AS TEMPLATE, DRILL THROUGH LID USING 1/4 INCH DRILL BIT

CABINET

Figure 9-6. Cabinet Machining Drawing

11. Place the cabinet on its side onto a sturdy table. Using the

oval slot in the striker plate as a template, use an electric

hand drill with a 1/4 inch diameter drill to drill through the

cover (be sure to make the slot in the cover the same size as

the slot in the striker plate) . Use a fine file to clean any

sharp edges.

12. To the blank side of the magnet, PN 67653, (the other side of

the magnet has a white dot) apply adhesive (PN 90702) . Insert

the magnet into the round hole in the striker plate so that

the white dot faces out.

c. Lid Latch Assembly Removal and Replacement

1. Place the cabinet upright. Remove and discard the cover plate

located in the front left corner of the cabinet, noting the

position of the two flat head screws for reassembly. Retain

the hardware.

2. Remove and retain the two flat head screws that secure the lid

latch assembly to the cabinet. From the outside of the

Rev. 11/90 9-17


cabinet, press the latching shaft in and lift the lid latch

assembly up and out of the cabinet. Discard the lid latch

assembly.

3. Using a 9/32 inch drill, enlarge the hole in the cabinet for

the latching shaft of the new lid latch assembly (refer to

figure 9-6 for proper location).

4. Clean the cover plate area before installing the new lid latch

assembly.

CAUTION

Be careful not to damage the sensor located on the

Latching Control P.C. Board when installing the new lid

latch.

5. Install the lid latch assembly (PN 04861), being careful not

to damage the sensor on the Latching Control P.C. Board. Apply

adhesive to the threads of the two flat head screws removed

in step 2, above. Tighten the screws to secure the lid latch

assembly in place.

NOTE

Check that the drain tubing and the saline-detector

tubing are properly positioned before securing the

cabinet to the chassis.

6. Replace the cabinet over the chassis and reconnect the lid

latch cable connector.

NOTE

Make sure the cable connector is properly and securely

connected.

Check that the wires on the chassis are not "pinched"

when the cabinet is put in place.

7. Secure cabinet to the chassis using the four 1/4-20 x 1-3/4

inch long socket head screws removed in step 5, Section b.

8. Connect the drain tubing and saline-detector tubing.

9. Place RTV-103 adhesive sealant (PN 67966) around the opening

for the cover plate.

10. Place the cover plate (PN 04852) supplied in position.

11. Apply adhesive (PN 90702) to the threads of the two flat head

screws removed in step 1. Place the screws into the screw holes

of the cover plate and loosely tighten.

9-18 Rev. 11/90


12. Align the remaining screw hole in the cover plate over the hole

inthe lid latch assembly. Use flat head screw (PN 67854) to

secure the cover plate in place. Securely tighten all three

screws.

13 . Attach the High Voltage Warning Label (PN 21990) to the cover

plate.

14. Replace the wet guard.

15. Replace the rotating bowl, rotor, and distributor.

9-5. Saline Shield Kit, PN 12811

a. Parts Supplied


Saline Shield 1

Seal Washer, SSt .260 ID x 1/2 OD 4

Cap Screw, Buttonhead, Socket,

1/4-20 x 2 Ig 4

Adhesive/ Sealant, Clear 1

04941

68772

68773

67971

b. Installation Instructions


top cover.

——————————————————————— WARNING ———————————————————————

Turn the power OFF and unplug the instrument power cord

before proceeding.

2. Remove the distributor, rotor and rotating bowl.

3. Lift out the wet guard.

4. Remove the four 1/4-20 socket head screws that secure the

cabinet to the chassis using a 3/16 inch Alien wrench. Discard

the screws.

5. Turn the instrument so the rear is facing you.

6. Place a bead of adhesive (PN 67971) along the top edge of the

saline shield (see figure 9-7 for proper placement).

Rev. 11/90 9-19


Lift the cabinet straight up until the bottom edge of the

cabinet is clear of the power supply adapter. Holding the

cabinet in one hand, position the saline shield into the cut¬

out area in the cabinet. Gently push up and pull forward on

the saline shield to securely bond it to the cabinet.

Lower the cabinet and secure it to the chassis using the cap

screws (PN 68773) and seal washers (PN 68772) supplied. Place

a seal washer on each cap screw (the rubber side of the washer

should be facing down) and tighten in place using a 5/32 inch

Alien wrench.

ADHESIVE BEAD

Figure 9-7. Adhesive Location

9-6. Triac-Interface Printed Circuit Board Replacement Kit, PN 12816

a. Parts Supplied


04834

04845

04851

04362

Triac-Interface P. C. Board Assembly

Triac Harness Assembly

Wet Guard Assembly, Front, Aluminum

Installation Instructions

b. Parts Removal


top cover.

WARNING

Because of the high voltages in the instrument, this

procedure must be done by a technician who is familiar

with electrical circuits. To remove the potential of

electrical shock, unplug the instrument power cord

before proceeding.

9-20 Rev. 11/90



3. Remove the distributor, rotor and rotating bowl from the

inside of the cellwasher chamber.

4. Lift out the chamber wet guard.

NOTE

Retain all hardware for reassembly of the instrument.

5. Disconnect the drain tubing and the saline-detector tubing.

6. Remove the four 1/4-20 x 1-3/4 inch long socket head screws


wrench.

NOTE

When lifting the cabinet up to disconnect the lid latch

cable connector, be careful not to stress the cable

connector.

7. Lift the cabinet up approximately four (4) inches. Then, while

holding the cabinet in one hand, reach in through the front

control panel opening, to the left rear corner of the

instrument and disconnect the lid latch cable connector by

squeezing the two locking tabs on the connector.

8. Lift the cabinet off the instrument chassis and place it

upright on a sturdy table or bench.

9 . From behind the front panel, remove the four (4 ) mounting nuts

(and washers) that secure the front panel to the chassis.

Remove the front panel/front wet guard assembly from the

chassis.

10. Disconnect the harness connector located at the back of the

front wet guard.

11. Remove the electrical tape at the top edge of the front panel

and front wet guard. Separate the front panel from the front

wet guard then disconnect connector located inside the front

wet guard. Discard front wet guard.

12. From the front panel, carefully disconnect the flex (ribbon)

cable between the interface PC board and the microcomputer PC

board. Remove the four screws and washers that secure the

interface PC board (PN 04536) in place (retain hardware for

installation of new triac-interface PC board assembly).

Discard interface PC board.

Rev. 11/90 9-21


c. Installation Procedure

1. Install the new triac-interface PC board assembly (PN 04834)

using hardware retained from step 12 above. Reconnect the flex

(ribbon) cable from the microcomputer PC board to the triac-

interface PC board.

CAUTION

Check that the triac harness assembly is properly

connected before reassembling the Cellwasher 2. If the

triac harness assembly is not properly connected the

triac-inter face PC board assembly will be damaged when

the Cellwasher is turned on.

2. Connect the keyed end (P20) of the triac harness assembly

(PN 04845) , to connector J20 on the triac-interface PC board.

Then connect the other end of the harness assembly (P21) to

J21 located in the aluminum front wet guard.

3. Connect the harness (P4) , in the aluminum front wet guard, to

pin connector J4 on the triac-inter face PC board.

4. Assemble the front panel and aluminum front wet guard by

inserting the studs on the front panel into the aluminum front

wet guard. Be sure to fold the antistatic electronic guard

(tab), located on the lower right corner of the front panel,

over the edge of the aluminum front wet guard and stud of the

front panel.

5. Replace electrical tape over the top edge of the front panel

and aluminum front wet guard.

6. Reconnect the harness connector to the back of the aluminum

front wet guard.

NOTE

To ensure electrical continuity, place a star washer on

the stud which has the antistatic electronic guard

(tab).

7. Place the aluminum front wet guard/front panel assembly into

the chassis. Secure the aluminum front wet guard/front panel

assembly in place using the four (4) mounting nuts (and

washers) removed in step 9, Section b.

NOTE

Check that the drain tubing and the saline-detector

tubing are properly positioned before securing the

cabinet to the chassis.

9.22 Rev. 11/90


8. Replace the cabinet over the chassis and reconnect the lid

latch cable connector.

NOTE

Make sure the cable connector is properly and securely

connected.

Check that the wires on the chassis are not "pinched"

when the cabinet is put in place.

9. Secure cabinet to the chassis using the four 1/4-20 x 1-3/4

inch long socket head screws removed in step 5, Section b.

10. Connect the drain tubing and saline-detector tubing.

11. Replace the chamber wet guard.

12. Replace the rotating bowl, rotor and distributor.

9-7. Adapter Clip Installation Kit, PN 04330

The adapter clips are designed to properly position the 10 mm x 75

mm tubes in the Sorvall DA-12 Rotor. Place an adapter clip into each

tube holder of the DA-12 Rotor as described on the next page.

NOTE

Adapter clips are not required when using 12 mm x 75 mm

tubes.

1. From the back of the tube holder, insert the narrow end of the

adapter clip into the top hole as shown in step 1 of figure

9-8.

2. Slide the adapter clip through the top hole until the wide end

of the clip is flush against the back of the tube holder, then

bring the narrow end of the adapter clip to align with the

bottom hole of the tube holder (see step 2 of figure 9-8).

3. Press the adapter clip so that the narrow end will snap into

the bottom hole of the tube holder (see step 3 of figure 9-

8).

4. Place a 10 mm x 75 mm tube into each tube holder. Make sure

that the tube is positioned so that the bottom of the tube rests

against the bottom of the tube holder.

5. Perform the run as explained in the cellwasher instruction

manual.

Rev. 11/90 9-23


Step 1

TOP HOLE

IN HOLDER

TUBE

HOLDER

WIDE END

ADAPTER

CLIP

Step 2

WIDE END

ADAPTER

CLIP

NARROW

END OF

CLIP

BOTTOM

HOLE IN

HOLDER

Step 3

SNAP

NARROW

END INTO

BOTTOM

HOLE

WIDE END

ADAPTER

CLIP

Figure 9-8. Installing an Adapter Clip

6. If using 12 mm x 75 mm tubes in the DA-12 Rotor or if the tube

holders of the rotor require cleaning/ remove the adapter

clips using a screwdriver (or similar object) and press

against the narrow end of the adapter clip. Pivot the adapter

clip and remove the clip from the top hole of tube holder.

9-24 Rev. 11/90

Section 10. Parts lists

This section contains the parts lists for the Cellwasher 2 asillustrated in figures 10-1 through 10-3.

NOTE

Tables 10-1 through 10-4 contain part numbers valid at the timeof printing. All part numbers are subject to change without notice.Part numbers in BOLD type are currently stocked at our partsdepot and are routinely available. Other parts listed will beprovided on a best effort basis.

Rev. 2/2002 10-1

CELLWASHER 2 SERVICE SORVALL® INSTRUMENTS

Table 10-1. SUGGESTED SPARE PARTS LIST


12975 Motor Assembly, 60 Hz (including new capacitor assy)

12976 Motor Assembly, 50 Hz (including new capacitor assy)

04527 Microcomputer PC Board Assembly

12816 Kit, Trial Interface for S/N 8503864 or Below

04834 Interface PC Board Assembly Only

04839 Triac PC Board Assembly

04543 Latch Control PC Board Assembly

12792 Lid Latch Assembly Replacement Kit

12977 Tubing Replacement Kit

43260 Distributor

04285 DA-12 Rotor

12796 Stainless Steel Rotating Bowl Assembly

12925 Flow Control Valve

69722 O-ring Seal for Collector Ring Assembly (S/N 8702302 and higher)

04353 Vinyl Seals for Collector Ring, 12/pkg. (S/N 8702301 and lower)

04552 Nozzle Assembly

Rev. 2/2002 10-2a


Table 10-1. SUGGESTED SPARE PARTS LIST (continued)


12981 Collector Ring

04538 Retainer, Collector Ring

12850 Retainer Ring (with o-ring)

04520 Pump Tubing (5/pkg)

04698 Hinge/Torsion Bar Assembly

67653 Magnet (Lid)

04570 Power Switch

67878 Power Transformer

43302 Pump Screw

04585 Nylon Washer

980031 Fuse/Amp (50 Hz) Motor Internal

980034 Fuse, 1.5 Amp (50 Hz) Transformer Internal

980039 Fuse, 3.0 Amp (50 Hz)

980044 Fuse, 6.25 Amp (60 Hz)

Rev. 2/2002 10-2b


Table 10-2. Cellwasher 2 Assembly (key to figure 10-1)

P/N (Qty) DESCRIPTION ITEM

1 12951 Cabinet Assembly Kit

2 04660 Cover Assembly

3 --- Chassis & Component Assembly, 50 Hz

3 --- Chassis & Component Assembly, 60 Hz

4 04552 Nozzle Assembly

5 12850 Retainer, Ring with O-Ring

--- 04538 Retainer, Collector Ring

6 04585 (4) Washer, Shoulder, Nylon

7 04833 (4) Thumbscrew, SSt, 1/4-20 x 3/8 Long; ASM #18024 SS=12

8 04698 Hinge/Torsion Bar Assembly

9 12981 Collector Ring

10 69722 O-ring Seal for Collector Ring

10 04353 (12/pkg)

Vinyl Seal for Collector Ring - old style

Rev. 2/2002 10-3


Table 10-2. Cellwasher 2 Assembly (continued) (key to figure 10-1)


11 12792 Lid Latch Assembly (only available in Service Kit)

12 04565 Guard, Lower Liner Wet (Black)

13 04852 Cover Plate

14 62422 Screw, Machine, Flat Head, Slotted SSt, UNC, #4-40 x 3/8" long

15 21990 Label, Warning High Voltage

16 --- Label, Serial Number

17 04550 Nameplate, Rating, 2.0A, 220V, 50 Hz

17 04551 Nameplate, Rating, 4.0A, 115V, 60 Hz

18 04941 Saline Shield

--- 12811 Saline Shield Kit (for older inst. Without shield)

19 68773 (4) Cap Screw, Button Head Socket, SSt, UNC, 1/4-20 x 2" long

20 68772 (4) Washer, Seal, SSt, .260 I.D. x 1/2 OD, Aztec Washer Co #n0500265

21 68826 (8) Screw, Machine, Flat Head, Self0Locking, SSt, Slotted, UNC, #6-32 x 7/16" Long

22 04599 Logo, Front Panel

23 67865 (2) Screw, Machine, Flat Head, Phillips, UNC, #6-32 x 1/4" long

24 04864 Latch Cover Plate

Rev. 2/2002 10-4a

CELLWASHER 2 SERVICE SORVALL ® INSTRUMENTS

Table 10-2. Cellwasher 2 Assembly (continued) (key to figure 10-1)

DESCRIPTION ITEM P/N

25 67653 Magnet, .250 Diameter x .100 thick, Permag Corp. #90C90A

26 67967 Screw, Machine, Hex Socket Flat Head, SSt, UNF, 4 48 x 3/16" long

28 --- Label, Warning, Fuse Replacement

29 04653 Rubber Boot

30 04543 Latch Control P.C. Board

31 93006 Fan Assembly

--- 67080 Power Cord, 3 Conductor 10A, 60 Hz

--- 91605 Power Cord, 3 Conductor, 10A, 50 Hz

Rev. 4/2005 10-4b

Administrator

Note

Accepted set by Administrator

Administrator

Note

None set by Administrator

Administrator

Line

Administrator

Line

Administrator

Line

Administrator

Line

Administrator

Line


Table 10-3. Chassis and Component Assembly (P/N 04540-60 Hz and P/N 04539-50 Hz)



1 04560 Insulator, Capacitor

2 60012** Nut, Hex, SSt, #10-32

3 61712 (5) Washer, Lock Internal, SSt, #10, .204 ID x .381 OD x .025 Thick

4 --- Insulation, Soundfoam, 3.0 x 3.0

5 --- Chassis Assembly

6 61539 (4) Screw, Machine, Pan Head, Slotted, SSt, UNC, #4-40 x 1/4" Long

7 63004 (4) Washer, Split Lock, SSt, #4, .020 Thick

8 04606 Plate, Connector, MTG

9 63013 (2) Washer, Split Lock, SST, #8, .031 Thick\

10 62358 Screw, Machine, Pan Head, Slotted SSt, UNC, #8-32 x 3/8" Long

11 67878 Transformer, Single Primary, 50/60 Hz 115V, with Thermo Protect; Signal #241-6-17-20

12 64771 (5) Screw, Machine, Pan Head, Slotted SSt, UNC, #6-32 x 3/4" Long

13 63011 (13) Washer, Split Lock, SSt, #6, .031 Thick ** Part Quantity required: 6 for 60Hz, 10 for 50Hz.

Rev. 2/2002 10-7


Table 10-3. Chassis and Component Assembly (P/N 04540-60 Hz and P/N 04539-50 Hz ) (continued)



14 12208 Rectifier (Sold in a kit)

15 60930 Screw, Machine, Pan Head, Slotted, SSt, UNC #6-32 x 1/4" Long

16 04620 Switch Assembly, Flow

17 60135 (4) Screw, Machine, Flat Head, Slotted, SSt, UNC, #8-32 x 3/8" Long

18 67632 Pump, Masterflex; Barnant #7017-21

19 04519 Guard, Line Cord Connector

20 04574 Connector Assembly

21 980044 Fuse, 6.25A, SLO-BLO, Type 3 AG, 250V Rating, 60 Hz

21 980039 Fuse, 3A, SLO-BLO, Type 3AG, 250V Rating, 50 Hz

22 04630 Transformer Assembly, 50 Hz ONLY

23 12977 ! Tubing Replacement Kit Includes: 1. Overflow/Vent/Discharge/Drain Tubing 2. Pump Tubing 3. Supply/Flow Tubing 4. Pump Connector 5. Y-Connector 6. Adjustable Tubing Clamp 7. Nylon Cable Tie 8. Installation Instructions

Rev. 2/2002 10-8


Table 10-3. Chassis and Component Assembly (P/N 04540-60 Hz and P/N 04539-50 Hz) (continued)



24 43302 (4) Screw, Pump Thumbscrew

25 43301 (4) Screw, Pump

26 04529 Bracket Assembly, Terminal Strip

27 61132 (4) Screw, Machine, Pan Head, Slotted, SSt, UNC, #6-32 x 3/8" Long

28 67628 Capacitor, High Capacity, 5800 MF; Mepco #86F528L

29 04570 Switch Assembly, Connector & Power

30 04834 Interface Printed Circuit Board (for s/n 8503865 and higher)

--- 12816 Kit,Triac Interface (for s/n 8503864 and lower)

31 04527 Microcomputer Printed Circuit Board Assembly Only

32 60074 (6) Nut, Hex, SSt, #6-32

33 60522 (2) Washer, Flat, SSt, 5/32 ID x 3/8 OD x .031 Thick

34 67663 Insulator, Heat sink, TO-3; Berquist #7403-09FR-03

35 67664 (2) Screw, Sheet Metal, Pan Head, Slotted, SSt, #6-20 x 1/2" Long, Type B

36 61711 (1) Washer, Lock Internal, #6.15 ID x .295 OD x .021 Thick

37 67078 Regulator, Voltage, Pos 5V; National Semiconductor #LM340K-5.0

Rev. 2/2002 10-9


Table 10-3. Chassis and Component Assembly (P/N 04540-60Hz and P/N 04539-50Hz) (continued)



38 12975 Motor Assembly, Drive, 115V, 60 Hz

38 12976 Motor Assembly, Drive, 115V, 50 Hz

39 04872 Seal, Drain Tray

40 04568 Capacitor Assembly, 60Hz (for G.E. motor only s/n 9803067)

40 04972 Capacitor Assembly, 60 Hz (for E.A.D. motor only s/n 9803068 and higher)

40 04510 Capacitor Assembly, 50 Hz (for G.E motor only s/n 9904729 and lower)

40 04974 Capacitor Assembly, 50Hz (for E.A.D. motor only s/n 9904730 and higher)

41 04371 Panel Assembly, Touch Pad

--- 04591 Panel Assembly complete, Touch Pad (Including microcomputer board and interface board)

42 68568 Bumper, Rubber, Recessed with Metal

43 63016 (2) Washer, Split Lock, #10, SSt, .040 Thick

44 66544 (4) Screw, Machine, Pan Head, Phillips with Washer, SSt, UNF, #10-32 x 7/8" long

45 04850 Aluminum Wet Guard Assembly (note: wet guard assy. Is included with 12816 Kit, Triac Interface)

46 04522 Protective Screen (cut-out for Fan)

Rev. 4/2005 10-10a


Table 10-3. Chassis and Component Assembly (P/N 04540-60Hz and P/N 04539-50Hz) (continued)



47 04542 Main Harness Assembly

48 04578 Motor Assembly, Pump, 50Hz / 60Hz

49 04517 Guard, Rear Wet, Machined

50 04845 Harness Assembly, Triac- Interface P.C. Board

51 61141 (3) Nut, Hex, SSt, #1/4-20

52 63019 (3) Washer, Split Lock, #1/4, SSt, .047 Thick

53 04865 Bracket Assembly (for G.E. motor only)

53 04881 Bracket Assembly (for E.A.D. motor only)

54 04839 Triac PC Board Assembly

55 980031 Fuse, 1.0 Amp (50 Hz only)

56 980034 Fuse, 1.5 Amp (50 Hz only)

57 92113 Fuse holder (50 Hz only)

58 04374 Adapter Harness (50 Hz only)

Rev. 2/2002 10-10b

CELLWASHER 2 SERVICE SORVALL® INSTRUMENTS Table 10-4. Drive Motor Assembly

(P/N 04575-60Hz and P/N 04576-50Hz) (key to figure 10-3)


--- 12975 Motor Assembly, Drive, 115V, 60 Hz

--- 12976 Motor Assembly, Drive, 115V, 50 Hz

1 04252 Clutch, Housing, Motor

2 04253 Clutch, Bushing, Motor

3 04874 Motor Drain Tray

4 04872 Motor Seal (used with old style drain tray p/n 04226 which has been replaced by 04874 that doesn’t use a motor seal. Change occurred in 1993)

5 04209 Loading Spring, Modified

6 04214 Decant Coil

7 04227 Motor Support Plate

8 04167 Motor Ground Wire

9 64746 Clutch, Roller for .500 Diameter Shaft; Torrington #RC 082308 Makes it Turn. Letters on Roller must fall out .

10 64795 Pin, Roll, Self-locking, 3/32 Diameter x 1" long, SSt; SPS #31-S-094-1.000

12 60495 Screw, Self-tapping, Round Head, Slotted, Steel Cad Plate, #6-32 x 3/8" long, Type F

13 63004 Washer, Split Lock, #4, SSt, .020 Thick

14 62914 Screw, Machine, Pan Head, Slotted, SSt, UNC, #4-40 x 7/8" Long

Rev. 2/2002 10-13


Table 10-4. Drive Motor Assembly (P/N 04575-60Hz and P/N 04576-50Hz)



15 60448 Washer, Split Lock, #1/4 X .045 Thick, Steel Cad Plate

16 61258 Screw, Cap, Hex Head, Steel Cad Plate, UNC, #1/4-20 x 5/8" Long

17 90983 Shock Mount, 1" Diameter x .75 Long 1/4-20; 60 Hz Only

17 67813 Shock Mount, 1” Diameter x .75 Long 1/4-20; 50Hz only

18 61141 Nut, Hex, SSt, #1/4-20

19 04287 Motor Drive Ring Subassembly Kit (Includes 5/64 hex key cut short for removal and installation)

20 61948 Washer, Flat, Nylon, .203 ID x .437 OC x .010 Thick

21 61257 Screw, Machine, Round Head, Slotted SSt, UNC, #6 32 x 3/16" Long l

22 61864 (2) Contact, Pin, Electrical Connector, Male 14-20 AWG; Molex #1190

23 61862 Connector Body, Plug, Electrical, 9-Position, Nylon, No Meeting Ears; Molex #03-09-2092

24 67669 Connector Body, Electrical, 3-Position, Nylon; Molex #03-09-2032

25 04260 Vane, Motor Cooling

Rev. 4/2005 10-14a


Table 10-4. Drive Motor Assembly (P/N 04575-60Hz and P/N 04576-50Hz)



26 60930 Screw, Machine, Pan Head, Slotted, SSt, UNC, #6-32 X ¼ Long

27 62021 Washer, Split Lock, Medium, #6, .031 Thick, 047 Section Width

28 04278 Washer, Shim, .005 Thick

29 04279 Washer, Shim, .010 Thick

30 04286 Spacer, 3/8” ID x 5/8 OD x .024 Thick

Rev. 2/2002 10-14b

DECONTAMINATION CERTIFICATE

Instructions on the reverse of this card must be completed before

field service or the return of the instrument or part to the Sorvall

service facility.

NAME__________________________ DEPARTMENT___________

INSTITUTION______________________ ADDRESS_____

CITY___________________________ STATE__________ ZIP

INSTRUMENT______________________ SERIAL NUMBER________

ROTOR________________________ SERIAL NUMBER________

PART___________________________ PART NUMBER

_________

CONTAMINATE USED__________________________

DECONTAMINATION CERTIFIED BY ___________________

DATE DECONTAMINATED ________________________




service facility.

NAME__________________________ DEPARTMENT___________

INSTITUTION______________________ ADDRESS_____________

CITY___________________________ STATE__________ ZIP

INSTRUMENT_______________________ SERIAL NUMBER________

ROTOR__________________________ SERIAL NUMBER________

PART___________________________ PART NUMBER

_________

CONTAMINATE USED__________________________

DECONTAMINATION CERTIFIED BY___________________





service facility.

NAME__________________________ DEPARTMENT___________

INSTITUTION______________________ ADDRESS_____________

CITY___________________________ STATE__________ ZIP

INSTRUMENT_______________________ SERIAL NUMBER ________

ROTOR________________________ SERIAL NUMBER________

PART__________________________ PART NUMBER

_________

CONTAMINATE USED_________________________

DECONTAMINATION CERTIFIED BY__________________


INSTRUCTIONS

l/hen an instrument that has been used with radioactive or pathogenic

laterial requires servicing by Sorvall personnel either at the customer's

iboratory or at Sorvall facilities, the following procedure must be corn-

lied with to insure safety of our personnel:

1. The instrument or part to be serviced shall be cleaned of all blood

and other encrusted material and decontaminated prior to servic¬

ing by our representative. No radioactivity shall be detectable by

survey equipment.

2. A Decontamination Information Certificate shall be completed and

attached to the instrument or part.

: an instrument or part to be serviced does not have a Decontamination

iformation Certificate attached to it, and, in our opinion, the instrument

'r part presents a potential radioactive or biological hazard, our repre-

entative will not service the equipment until proper decontamination

,nd certification has been completed. If an instrument is received at our

Service facilities and, in our opinion, is a radioactive or biological hazard,

the sender will be contacted for instructions as to disposition of the

equipment. Disposition costs will be borne by the sender.

Decontamination Information Certificates are included with these Opera¬

tion Instructions. Additional certificates are available from your local

technical or customer service representative. In the event these certifi¬

cates are not available, a written statement certifying that the instrument

or part has been properly decontaminated and outlining the procedures

used will be acceptable.

NOTE

Service representatives will indicate on a Customer

Service Repair Report if decontamination was required,

and if so, what the contaminate was and what procedure

was used. If no decontamination was required, it should

be so stated. 4/97

INSTRUCTIONS

Vhen an instrument that has been used with radioactive or pathogenic

riaterial requires servicing by Sorvall personnel either at the customer's

iboratory or at Sorvall facilities, the following procedure must be com-

ilied with to insure safety of our personnel:




survey equipment.



f an instrument or part to be serviced does not have a Decontamination

^formation Certificate attached to it, and, in our opinion, the instrument

ir part presents a potential radioactive or biological hazard, our repre-

.entative will not service the equipment until proper decontamination

ind certification has been completed. If an instrument is received at our










NOTE





be so stated. ,1/07

INSTRUCTIONS

Vhen an instrument that has been used with radioactive or pathogenic

naterial requires servicing by Sorvall personnel either at the customer's

aboratory or at Sorvall facilities, the following procedure must be com-

>lied with to insure safety of our personnel:




survey equipment.



f an instrument or part to be serviced does not have a Decontamination

nformation Certificate attached to it, and, in our opinion, the instrument

>r part presents a potential radioactive or biological hazard, our repre-

.entative will not service the equipment until proper decontamination

ind certification has been completed. If an instrument is received at our










NOTE





be so stated. ^

AUSTRIA

Kendro Laboratory Products GmbH

WiefelestraBe 4

A-1230Wien

Tel.: 43(1)80140-0

Fax: 43(1)80140-40

e-mail: [email protected]

FRANCE

Kendro Laboratory Products

B.P. 244, Pare Hightec 6

Batiment Ie Meridien

9 Avenue du Canada

Paris

F-91944 COURTABOEUF Cedex

Tel.: 33(1)69187777

Fax: 33(1)60920034


GERMANY


Heraeusstrasse 12-14

D-63450 Hanau

Tel.: 49(6181)35-300

Fax: 49(6181)35-6944


ITALY

AHSI S.p.A.

Via dei Chiosi, 11

1-20040 Cavenago Brianza (Milan)

Tel.: 39(2)950811

Fax: 39(2)95081277


POLAND

Heraeus Sp. zo.o.

ul. Duchnicka 3

PL-01-796 Warszawa

Tel.: 48(22)6634323

Fax: 48(22)6634325

e-mail; [email protected]

SWEDEN

Kendro Laboratory Products AB

Hagangsvagen 32

S-19445 Upplands Vasby

Tel.: 46 (8) 59 07 21 90

Fax: 46(8)59031600


SWITZERLAND

Kendro Laboratory Products AG

Raffelstrasse 32-Postfach

CH-8045 Zurich

Tel.: 41(1)4541212

Fax: 41(1)4541299


UNITED KINGDOM

Kendo Laboratory Products Limited

Stortford Hall Park

Bishop's Stortford

Hertfordshire CM23 5GZ

Tel.: 44(1279)827700

Fax: 44(1279)827750


Other EUROPE, MIDDLE EAST,

or AFRICA:


Heraeusstrasse 12-14

D-63450 Hanau

GERMANY

Tel.: 49(6181)35-300

Fax: 49(6181)35-5944


AUSTRALIA

Kendro Laboratory Products Pty. Ltd.

Building 4,2-6 Orion Road

Lane Cove, Sydney, NSW 2066

Tel.: 61(2)99361540

Fax: 61 (2) 9427 9765


CHINA, BEIJING:

INSTEC-Kendro Beijing Service Station

Room N1203, Jing Bao Garden

183 An Ding Men Wai St.

Beijing 100011, P. R.China

Tel: 86(10)6426-2609

Fax: 86(10)6426-2765


CHINA, HONG KONG:

Kendro Laboratory Products (H.K.) Limited

Room 305, Wing On Plaza

62 Mody Road

Tsimshatsui East, Kowloon

Hong Kong, S.A.R. China

TeL: (852)2142-3910

Fax: (852)2711-3858

CHINA, SHANGHAI:

INSTEC-Kendro Shanghai Service Station

Room 22G, Hui Jia Building

No. 41 Cao Xi Bei Lu

Shanghai 200030, P.R. China

Tel.: 86(21)5490-0216

Fax: 86(21)5490-0230


INDIA

Kendro Laboratory Products (India) Pvt. Ltd.

B-5/75 (LGF) Safdarjung Enclave

New Delhi, IND-110029

Tel.: 91(11)6184840

Fax: 91(11)6185397


Other ASIA PACIFIC:

Kendro Laboratory Products (H.K.) Limited

Room 305, Wing On Plaza

62 Mody Road

Tsimshatsui East, Kowloon

Hong Kong, S.A.R. China

Tel.: (1352)2142-3910

Fax: (852)2711-3858

CANADA


31 Pecks Lane

Newtown, CT 06470-2337

U.S.A.

Tel.: (203) 270-2080

Fax: (203)270-2166


UNITED STATES Of AMERICA


31 Pecks Lane


Tel.: (800) 522-7746 toll-free

Fax: (203)270-2166

(203)270-2115

(203)270-2110


Other countries, including

LATIN AMERICA:


31 Pecks Lane


U.S.A.

Tel.: (203) 270-2080

Fax: (203)270-2210

(203)270-2166


Or contact a local representative for SORVALL* brand products. Visit our web site at http://www.lwinlro.com or http://www.kendro.de

SORVALL:

RBV.7/W

•(Kendro

Laboratory Products

Cellwasher 2 Cell Washing System

Documents

Transcript of Cellwasher 2 Cell Washing System