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Cell Culture Media and Reagents Product Selection Guide

cellgro ®

ContentsClassical Media 5

CMRL 1066 7Dulbecco’s Modification of Eagle’s Medium (DMEM) 7DMEM/Ham’s F-12 50/50 Mix (Mod.) 11F-12K Nutrient Mixture (Kaighn’s Mod.) 12Glasgow’s MEM 12Ham’s F-10 Medium 13Ham’s F-12 Medium 13Improved MEM (Richter’s Mod.) 14Iscove’s Modification of DMEM 14Leibovitz’s L-15 (Mod.) 15MCDB 131 15McCoy’s 5A (Iwakata and Grace Modification) 15Medium 199 (Mod.) 16Minimum Essential Medium (MEM) Alpha Medium 17Minimum Essential Medium (MEM) 18RPMI 1640 20

Buffered Salt Solutions 23Dulbecco’s Phosphate Buffered Saline (DPBS) 25Phosphate Buffered Saline (PBS) 26Hanks’ Balanced Salt Solution (HBSS) 26Phosphate Buffered Saline (PBS) 26Hanks’ Balanced Salt Solution (HBSS) 26

Serum-Free and Speciality Media 31cellgro COMPLETE™ 32insectagro DS2™ 33insectagro™ 33insectagro Sf9™ 33transfectagro™ 33 Lymphocyte Separation Medium 34Polysucrose 400 34Tryptic Soy Broth (TSB) 35cellgro® rhAlbumin 35cellgro® rhLactoferrin 36stemgro® AB2™ Neural Base Media 36stemgro® ANS™ Neural Supplement 36

Human and Animal Sera 39Fetal Bovine Serum 43Donor Horse Serum 45Human AB Serum 45Porcine Serum 45Charcoal-Stripped FBS 46Dialyzed FBS 46ES Cell Tested FBS 46Gamma-Irradiated FBS 47Low IgG FBS 47Tetracycline Negative FBS 47

Cell Culture Reagents 49L-glutamine 51glutagro™ 51MEM Amino Acids 52MEM Vitamins 52Antibiotics and Antimycotics 52Buffers 56Trypsin 57Non-Enzymatic Cell Dissociation Agents 58Growth Supplements 58Hybridoma Reagents 58Miscellaneous Reagents 59

Insect Media and Reagents 63Grace’s Insect Basal Medium 64Hink’s TNM-FH Medium 64insectagro™ Reagents 64insectagro™ Serum-Free Media 65

Microbiology and Molecular Biology 67Miller’s LB Broth 68S.O.C Medium 68Terrific Broth 68Ampicillin 68Carbenicillin 68Kanamycin Sulfate 68Neomycin Sulfate 69Streptomycin Sulfate 69Tetracycline Hydrochloride 69X-Gal 70Isopropylthiogalactoside (IPTG) 70Phosphate Buffered Saline (PBS), 10x 71SSC, 20x, Liquid 71SSPE, 20x, Liquid 71TAE Buffer, 10x 72TBE Buffer, 10x, Liquid 72TE Buffer, 1x, Liquid 72Tris Buffered Saline, 10x, Liquid 73Tris-Hydrochloride Buffers, 1M 73EDTA, 0.5M, pH 8.0 74Molecular Biology Grade Water 74Reagent Grade Water, 4L Cube 74MOPS Buffer 74SDS (Sodium Dodecyl Sulfate), 10% 75Sodium Acetate, 3M 75Sodium Chloride, 5M 75

High Quality Water 77Distilled, Deionized Water 79USP/EP Certified Sterile WFI Quality Water 79Sterile WFI Quality, Cell Culture Grade Water 79

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C L A S S I C A L M E D I AClassical M

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Classical Cell Culture Media

CMRL 1066 7DMEM 7DMEM/Ham’s F-12 50/50 Mix (Mod.) 11F-12K Nutrient Mixture (Kaighn’s Mod.) 12Glasgow’s MEM 12Ham’s F-10 Medium 13Ham’s F-12 Medium 13Improved MEM (Richter’s Mod.) 14Iscove’s Modification of DMEM 14Leibovitz’s L-15 (Mod.) 15MCDB 131 15McCoy’s 5A (Iwakata and Grace Modification) 15Medium 199 (Mod.) 16Minimum Essential Medium (MEM) 17RPMI 1640 20

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C L A S S I C A L M E D I ACl

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Visit www.vwr.com for additional product and technical information.

Classical MediaIntroductionGrowth Promotion and CytotoxicityCorning cellgro products undergo a variety of tests in which functionality and toxicity are determined using growth-promotion analysis. Product performance is measured by harvest to plant ratios or fold increase according to predetermined specifications.

Physiochemical PropertiesLiquid and powder products undergo testing to determine pH and osmolality. Powders are also tested for residual moisture. The pH and osmolality tests are performed according to specific protocols in which all equipment is calibrated using standards which are traceable to the National Institute of Standards and Technology.

Biological TestsTesting of cellgro products guarantee low levels of endotoxin, mycoplasma and an absense of contamination.

Endotoxin: Media are tested for bacterial endotoxin using the Limulus Amoebocyte Lysate (LAL) Chromogenic Assay. For product release, each lot of standard liquid media must have an endotoxin level of < 0.25 EU/mL. Powder products must have an endotoxin level of < 0.25 EU/mL when reconstituted to formula weight with low-endotoxin water.

Sterility: All cellgro products are sterility tested.

Mycoplasma: Media products are tested for mycoplasma offsite using a large-volumemethod described by Kern and Barile in “Isolation of Mycoplasmas from Cell Culture by Agar and Broth Techniques,” M.F. Barile and G.J. McGarrity (1983) in Methods of Mycoplasmology, Vol. 11,5. Razin and J.G. Tulley, eds., New York: Academic Press, 159-165.

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CMRL 1066CMRL 1066 is a modified version of Medium 199 and was designed initially for used with L-strain cells in unsupplemented culture. It has been used to maintain monolayer growth of permanent cell lines for extended periods of time without protein supplementation. Although developed for use in serum-free cell culture, CMRL 1066 can be supplemented with serum and used to support the growth of many types of cells.

� Without L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.

Corning Cat. No. Size Qty/Pk Shipping Storage Expires

45001-114 15-110-CV 500 mL 6 15-30ºC 2-8ºC 36 Months

Dulbecco’s Modification of Eagle’s Medium (DMEM)Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of normal and transformed cells, such as primary cultures of mouse and chicken cells.

Contains

45000-30645000-30445000-31045000-308

45000-33445000-332

45000-31445000-312

45000-31845000-31645000-32245000-320

45000-32645000-324

L-glutamine ¢ ¢ ¢

Sodium pyruvate ¢ ¢ ¢

Phenol red ¢ ¢ ¢ ¢ ¢

L-Cystine and L-Methionine ¢ ¢ ¢ ¢ ¢

Phosphate ¢ ¢ ¢ ¢ ¢

HEPES

High glucose ¢ ¢ ¢ ¢

Contains45000-33045000-328 45000-340 45000-336 45000-342 97060-876

L-glutamine

Sodium pyruvate ¢ ¢ ¢ ¢

Phenol red ¢ ¢ ¢ ¢

L-Cystine and L-Methionine ¢ ¢ ¢ ¢

Phosphate ¢ ¢ ¢ ¢

HEPES ¢

High glucose ¢ ¢ ¢ ¢

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Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x � With 4.5 g/L glucose, L-glutamine, and sodium pyruvate � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-304 10-013-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

45000-306 10-013-CM 1L 6 15-30ºC 2-8ºC 18 Months

45000-308 10-013-LX 10L 1 15-30ºC 2-8ºC 18 Months

45000-310 10-013-LB 20L 1 15-30ºC 2-8ºC 18 Months

Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x � With 1.0 g/L glucose, sodium pyruvate, L-glutamine and phenol red � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-332 10-014-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

45000-334 10-014-CM 1L 6 15-30ºC 2-8ºC 18 Months

Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x � With 4.5 g/L glucose and L-glutamine, without sodium pyruvate � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-312 10-017-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

45000-314 10-017-CM 1L 6 15-30ºC 2-8ºC 18 Months

Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x � With 4.5 g/L glucose and sodium pyruvate without L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-316 15-013-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

45000-318 15-013-CM 1L 6 15-30ºC 2-8ºC 18 Months

45000-320 15-013-LX 10L 1 15-30ºC 2-8ºC 18 Months

45000-322 15-013-LB 20L 1 15-30ºC 2-8ºC 18 Months

Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x � With 4.5 g/L glucose, without L-glutamine and sodium pyruvate � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-324 15-017-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

45000-326 15-017-CM 1L 6 15-30ºC 2-8ºC 18 Months

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Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x � With 4.5 g/L glucose, sodium pyruvate, and 25 mM HEPES, without L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-328 15-018-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

45000-330 15-018-CM 1L 6 15-30ºC 2-8ºC 18 Months

Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x � With 4.5 g/L glucose and sodium pyruvate, without L-glutamine, L-methionine and L-cystine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-340 17-204-CI 100 mL 6 15-30ºC 2-8ºC 18 Months

Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x � With 4.5 g/L glucose, and sodium pyruvate, without L-glutamine and phenol red � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-336 17-205-CV 100 mL 6 15-30ºC 2-8ºC 18 Months

Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x � With 4.5 g/L glucose and sodium pyruvate, without L-glutamine and phosphate � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-342 17-206-CI 100 mL 6 15-30ºC 2-8ºC 18 Months

Dulbecco’s Modification of Eagle’s Medium (DMEM), 1x � Without glucose, L-glutamine, and sodium pyruvate � Intended Use: In Vitro Diagnostic

VWR Cat. No.


97060-876 17-207-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

glutagro™ DMEM � Dulbecco’s Modification of Eagle’s Medium, 1x with glutagro supplement, 4.5 g/L glucose, sodium pyruvate and phenol red

� Intended Use: In Vitro Diagnostic

VWR Cat. No.


71002-872 10-101-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

glutagro™ DMEM � Dulbecco’s Modification of Eagle’s Medium, 1x with glutagro supplement, 4.5 g/L glucose and phenol red, without sodium pyruvate


VWR Cat. No.


71002-874 10-102-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

New

New

New

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Dulbecco’s Modification of Eagle’s Medium (DMEM), Powder

Contains45000-51845000-520

45000-52245000-524 45000-526 45000-534

L-glutamine ¢ ¢

Sodium pyruvate ¢

Phenol red ¢ ¢

L-Cystine and L-Methionine ¢ ¢ ¢ ¢

Phosphate ¢ ¢ ¢ ¢

HEPES

High glucose ¢ ¢ ¢

Pyridoxine HCl ¢ ¢ ¢ ¢

Sodium bicarbonate

Dulbecco’s Modification of Eagle’s Medium (DMEM), Powder � With 4.5 g/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-518 50-003-PB 10L 1 15-30ºC 2-8ºC 36 Months

45000-520 50-003-PC 50L 1 15-30ºC 2-8ºC 36 Months

Dulbecco’s Modification of Eagle’s Medium (DMEM), Powder � With 4.5 g/L glucose and L-glutamine, without sodium bicarbonate and sodium pyruvate � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-522 50-013-PB 10L 1 15-30ºC 2-8ºC 36 Months

45000-524 50-013-PC 50L 1 15-30ºC 2-8ºC 36 Months

Dulbecco’s Modification of Eagle’s Medium (DMEM), Powder � With 4.5 g/L glucose, without sodium bicarbonate, L-glutamine, sodium pyruvate and phenol red


VWR Cat. No.


45000-526 90-013-PB 10L 1 15-30ºC 2-8ºC 36 Months

Dulbecco’s Modification of Eagle’s Medium (DMEM), Powder � Without sodium bicarbonate, glucose, L-glutamine, sodium pyruvate, and phenol red � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-534 90-113-PB 10L 1 15-30ºC 2-8ºC 36 Months

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DMEM/Ham’s F-12 50/50 Mix (Mod.)This 1:1 mixture of Dulbecco’s Modified Eagle Medium and Ham’s F-12 media is ideal for culture of a variety of cell types under low serum conditions. Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. Ham’s F-12 media is based on Ham’s F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids.

Contains71002-67445000-344

45000-35245000-350

45000-34845000-346 45001-052

45000-54045000-542

45000-53645000-538


Sodium pyruvate ¢ ¢ ¢ ¢ ¢ ¢


HEPES ¢

Sodium bicarbonate ¢ ¢ ¢ ¢

Dulbecco’s Modification of Eagle’s Medium/Ham’s F-12 50/50 Mix, 1x (Mod.) � With L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-344 10-090-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

71002-674 10-090-CM 1L 6 15-30ºC 2-8ºC 12 Months

Dulbecco’s Modification of Eagle’s Medium/Ham’s F-12 50/50 Mix, 1x (Mod.) � With L-glutamine, without phenol red � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45001-052 16-405-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

Dulbecco’s Modification of Eagle’s Medium/Ham’s F-12 50/50 Mix, 1x (Mod.) � Without L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-346 15-090-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

45000-348 15-090-CM 1L 6 15-30ºC 2-8ºC 18 Months

Dulbecco’s Modification of Eagle’s Medium/Ham’s F-12 50/50 Mix, 1x (Mod.) � With L-glutamine and 15 mM HEPES � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-350 10-092-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

45000-352 10-092-CM 1L 6 15-30ºC 2-8ºC 12 Months

12


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DMEM/Ham’s F-12 50/50 Mix (Mod.)

Dulbecco’s Modification of Eagle’s Medium/Ham’s F-12 50/50 Mix, 1x (Mod.) � Without sodium bicarbonate and L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.

Corning Cat. No.

Size Qty/Pk Shipping Storage Expires

45000-536 90-091-PB 10L 1 15-30ºC 2-8ºC 36 Months

Dulbecco’s Modification of Eagle’s Medium/Ham’s F-12 50/50 Mix, 1x (Mod.) � Without sodium bicarbonate, L-glutamine, and phenol red � Intended Use: In Vitro Diagnostic

VWR Cat. No.

Corning Cat. No.


45000-540 90-090-PB 10L 1 15-30ºC 2-8ºC 36 Months

glutagro™ DMEM/F-12 � Dulbecco’s Modification of Eagle’s Medium/Ham’s F-12 50/50 Mix with glutagro � Intended Use: In Vitro Diagnostic

VWR Cat. No.

Corning Cat. No.


71002-876 10-103-CV 500 mL 6 15-30ºC 2-8ºC 36 Months

F-12K Nutrient Mixture (Kaighn’s Mod.)A modification of Ham’s F-12, F-12K contains higher concentrations of amino acids, sodium pyruvate and other components than the original. It is formulated to support the growth of cells including differentiated rat and chicken cell types and primary human hepatocytes.

F-12K Nutrient Mixture (Kaighn’s Mod.) 1x � With L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.

Corning Cat. No.


45000-354 10-025-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

Glasgow’s MEMGlasgow’s MEM is a modification of MEM formulated to support the growth of BHK-21 cells. The medium was originally based on BME with the addition of 10% tryptose phosphate broth and two times the concentration of amino acids and vitamins.

Glasgow’s MEM, Powder � With L-glutamine, without sodium bicarbonate and tryptose phosphate broth � Intended Use: In Vitro Diagnostic

VWR Cat. No.

Corning Cat. No.


45000-544 50-004-PB 10L 1 15-30ºC 2-8ºC 36 Months

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Ham’s F-10 MediumHam’s F-10 is a classical media designed to support the growth of a variety of cell types, including HeLa, Chinese Hamster Overy (CHO), primary cells from tissue explants and various types of human diploid cells. Serum supplementation may or may not be necessary depending on the type of cell being cultured.

Dulbecco’s Modification of Eagle’s Medium/Ham’s F-12 50/50 Mix, 1x (Mod.) � With L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.

Corning Cat. No.


45000-356 10-070-CV 500 m L 6 15-30ºC 2-8ºC 12 Months

Ham’s F-12 MediumHam’s F-12 media was originally formulated for the serum-free culture of CHO cells, and is based on Ham’s F-10 medium with increased concentrations of choline, inositol, putres-cine, and several amino acids. This medium is also suitable for carcinoma cells, rat skeletal myoblasts, Chinese hamster lung cells, and rat, rabbit, and chicken embryos.

Ham’s F-12 Medium, 1x � With L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.

Corning Cat. No.


45000-358 10-080-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

45000-360 10-080-CM 1L 6 15-30ºC 2-8ºC 12 Months

Ham’s F-12 Medium, Powder � With L-glutamine, without sodium bicarbonate � Intended Use: In Vitro Diagnostic

VWR Cat. No.

Corning Cat. No.


45000-550 50-040-PB 10L 1 15-30ºC 2-8ºC 36 Months

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Improved MEM (Richter’s Mod.)A modified forumulation of Minimal Essential Medium, Improved Minimum Essential Medium (IMEM) contains a higher concentration of many components used in MEM along with additional amino acids, vitamins, and metabolites.

Improved MEM (Richter’s Mod.), 1x � With L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-362 10-024-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

Improved MEM (Richter’s Mod.), 1x � With L-glutamine, without phenol red � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-364 10-026-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

Iscove’s Modification of DMEMIscove’s Modification of DMEM (IMDM) is a highly enriched synthetic medium optimized for rapidly proliferation cultures of high cell density. A modified form of Dulbecco’s Modi-fied Eagle Medium (DMEM), Iscove’s contains additional amino acids, vitamins, selenium and HEPES buffer, and is formulated with potassium nitrate in place of ferric nitrate. The addition of albumin and transferrin may be required for certain cell types.

Contains45000-36845000-366 45000-370

L-glutamine ¢

Phenol red ¢ ¢

Sodium Pyruvate ¢ ¢

Pyridoxine HCl ¢ ¢

Sodium Bicarbonate ¢ ¢

Iscove’s Modification of DMEM, 1x, liquid � With L-glutamine and 25 mM HEPES, without ß-thioglycerol and ß-mercaptoethanol � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-366 10-016-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

45000-368 10-016-CM 1L 6 15-30ºC 2-8ºC 12 Months

Iscove’s Modification of DMEM, 1x, liquid � With 25 mM HEPES, without ß-thioglycerol, ß-mercaptoethanol, and L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-370 15-016-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

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Leibovitz’s L-15 (Mod.)Leibovitz’s L15 medium was originally developed without a sodium bicaronate buffer system for use in non-CO2 equilibrated environments. It is buffered by phosphates and the amino acid L-arginine, along with L-histidine and L-cysteine. The formation of acid metabolite byproducts is prevented by replacing glucose with galactose and sodium pyruvate. Examples of usage include the propagation of HEP-2, monkey kidney cells and primary explants of embryonic and adult human tissue. Leibovitz’s L-15 supports established cell lines as well as primary explants of embryonic and adult human tissue.

Leibovitz’s L-15 (Mod.) 1x � With L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-372 10-045-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

Leibovitz’s L-15 (Mod.) 1x, powder � With L-glutamine, without phenol red � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-562 50-045-PB 10L 1 15-30ºC 2-8ºC 12 Months

MCDB 131MCDB 131 was originally developed by the researchers Knedler and Ham for the low-protein and serum free growth of human microvascular endothelial cells (HMVEC). Since its development, this medium has been used for this cell type and others such as liver, smooth muscle, cardiac myocytes. It is one of a group of MCDB media which were designed to provide a defined and optimally balanced nutritional environment for various mammalian cell types.

MCDB 131, 1x � Without L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45001-112 15-100-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

McCoy’s 5A (Iwakata and Grace Modification)McCoy’s 5A medium was originally formulated to meet the culture requirements of Novikoff Hepatoma cells, and has been found to support the indefinite proliferation of Walker 256 carcinoma cells along with a wide range of human and rat normal and transformed cells. Originally modified by Iwakata and Grace, this version of McCoy’s 5A includes increased levels of inositol and glucose and contains L-glutamine.

(con’t)

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McCoy’s 5A (Iwakata and Grace Modification)

Contains 45000-374 45000-376

L-glutamine ¢ ¢

Phenol red ¢ ¢

HEPES ¢


McCoy’s 5A (Iwakata and Grace Modification), 1x � With L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-374 10-050-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

McCoy’s 5A (Iwakata and Grace Modification), 1x � With L-glutamine and 25 mM HEPES � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-376 10-051-CI 100 mL 6 15-30ºC 2-8ºC 18 Months

Medium 199 (Mod.)Medium 199 is often used in vaccine production, virology, and for the culture of various non-transformed cells. It was originally developed as the result of efforts to create a fully defined, animal origin-free medium. Long-term use of this medium requires serum supplementation. All components are animal origin-free.

Contains 45000-378 45000-570 45000-578 45000-574


Phenol red ¢ ¢ ¢

Earle’s Salts ¢ ¢ ¢

Hanks’ Salts ¢


Medium 199 (Mod.), 1x � With Earle’s salts and L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-378 10-060-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

Medium 199 (Mod.), Powder � With Earle’s salts, L-glutamine, sodium bicarbonate � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-570 50-050-PB 10L 1 15-30ºC 2-8ºC 18 Months

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Medium 199 (Mod.), Powder � With Earle’s salts, without sodium bicarbonate, L-glutamine, and phenol red � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-574 90-050-PB 10L 1 15-30ºC 2-8ºC 36 Months

Medium 199 (Mod.), Powder � With Hanks’ salts, L-glutamine, sodium bicarbonate � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-578 50-051-PB 10L 1 15-30ºC 2-8ºC 36 Months

Minimum Essential Medium (MEM) Alpha MediumThis is an enriched variation of Minimum Essential Medium, containing all 21 normal amino acids, and 5 additional vitamins. Minimum Essential Medium (MEM) is a common cell culture medium developed from early work with Basal Medium Eagle (BME). It is modified with higher concentrations of amino acids to more closely approximate the protein composi-tion of mammalian cells. When supplemented with serum, MEM has been used for cultiva-tion of a wide variety of cells grown in monolayers, such as fibroblasts.

Contains 45000-300 45000-302 45000-504

L-glutamine ¢ ¢

Phenol red ¢ ¢ ¢

Nucleosides ¢

Sodium Pyruvate ¢ ¢ ¢


Minimum Essential Medium (MEM) Alpha Medium, 1x � With Earle’s salts, ribonucleosides, deoxyribonucleosides, and L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-300 10-022-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

Minimum Essential Medium (MEM) Alpha Medium, 1x � With Earle’s salts, without ribonucleosides, deoxyribonucleosides, and L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-302 15-012-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

Minimum Essential Medium (MEM) Alpha Medium, Powder � With Earle’s salts and L-glutamine, without ribonucleosides, deoxyribonucleosides, and sodium bicarbonate


VWR Cat. No.


45000-504 50-012-PC 50L 1 15-30ºC 2-8ºC 36 Months

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Minimum Essential Medium (MEM)Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells. This research indicated that additions made to BME aided cell propagation. MEM is modified with higher concentrations of amino acids to more closely approximate the protein composition of mammalian cells. When supplemented with serum, MEM has been used for cultivation of a wide variety of cells grown in monolayers, such as fibroblasts.

Contains45000-38245000-380

45000-38645000-384 45000-394 45000-388

L-glutamine ¢

Phenol red ¢ ¢ ¢

L-Methionine ¢ ¢ ¢ ¢

Calcium and magnesium ¢ ¢ ¢

Sodium Bicarbonate ¢ ¢ ¢ ¢

Minimum Essential Medium (MEM), 1x � With Earle’s salts and L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-380 10-010-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

45000-382 10-010-CM 1L 1 15-30ºC 2-8ºC 18 Months

Minimum Essential Medium (MEM), 1x � With Earle’s salts without L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-384 15-010-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

45000-386 15-010-CM 1L 1 15-30ºC 2-8ºC 18 Months

Minimum Essential Medium (MEM), 1x � With Earle’s salts, without L-glutamine and phenol red � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-388 17-305-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

Minimum Essential Medium (MEM), 1x � Without L-glutamine, calcium, and magnesium � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-394 15-015-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

19


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Minimum Essential Medium (MEM), Powder

Contains45000-58245000-584

45000-59445000-596 45000-592 45000-586

L-glutamine ¢

Phenol red ¢ ¢ ¢

Earle’s Salts ¢ ¢ ¢ ¢

Minimum Essential Medium (MEM), Powder � With Earle’s salts and L-glutamine, without sodium bicarbonate � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-582 50-010-PB 10L 1 15-30ºC 2-8ºC 36 Months

45000-584 50-010-PC 50L 1 15-30ºC 2-8ºC 36 Months

Minimum Essential Medium (MEM), Powder � With Earle’s salts, without L-glutamine, phenol red, and sodium bicarbonate � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-586 90-009-PB 10L 1 15-30ºC 2-8ºC 36 Months

Minimum Essential Medium (MEM), Powder � With Earle’s salts, without L-glutamine and sodium bicarbonate � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-592 51-010-PC 50L 1 15-30ºC 2-8ºC 36 Months

Minimum Essential Medium (MEM), Powder � With Earle’s salts, L-glutamine, and nonessential amino acids, without sodium bicarbonate � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-594 50-011-PB 10L 1 15-30ºC 2-8ºC 36 Months

45000-596 50-011-PC 50L 1 15-30ºC 2-8ºC 36 Months

20


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RPMI 1640

Named for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy’s 5A containing an increased level of inositol. Examples of cell types for which this medium is appropriate include mammalian myeloma and hybridoma cells such as mouse hybridomas, human leukocytes, and B and T lymphocytes. RPMI 1640 is also used for suspension cultures or monolayer human leukemic cells.

Contains 45000-39845000-41445000-412 45001-110

45000-40645000-404 45000-408 45000-418 45000-416 45000-410

45000-60245000-604

L-glutamine ¢ ¢ ¢ ¢

Phenol red ¢ ¢ ¢ ¢ ¢ ¢ ¢

HEPES ¢ ¢

L-Cystine and L-Methionine ¢ ¢ ¢ ¢ ¢ ¢ ¢

Sodium Bicarbonate ¢ ¢ ¢ ¢ ¢ ¢ ¢

RPMI 1640, 1x � With L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-396 10-040-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

45000-398 10-040-CM 1L 6 15-30ºC 2-8ºC 18 Months

45000-400 10-040-LX 10L 1 15-30ºC 2-8ºC 18 Months

45000-402 10-040-LB 20L 1 15-30ºC 2-8ºC 18 Months

RPMI 1640, 1x � With glutagro supplement and phenol red � Intended Use: In Vitro Diagnostic

VWR Cat. No.


71002-878 10-104-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

RPMI 1640, 1x � Without L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-404 15-040-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

45000-406 15-040-CM 1L 6 15-30ºC 2-8ºC 18 Months

45000-408 15-040-LB 20L 1 15-30ºC 2-8ºC 18 Months

New

21


edia

RPMI 1640, 1x � Without L-glutamine and phenol red � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-410 17-205-CV 500 mL 6 15-30ºC 2-8ºC 18 Months RPMI 1640, 1x

� With L-glutamine and 25 mM HEPES � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-412 10-041-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

45000-414 10-041-CM 1L 6 15-30ºC 2-8ºC 18 Months

RPMI 1640, 1x � With L-glutamine, without glucose � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45001-110 10-043-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

RPMI 1640, 1x � With 25 mM HEPES, without L-glutamine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-418 15-041-CV 500 mL 6 15-30ºC 2-8ºC 18 Months

RPMI 1640, 1x � Without L-glutamine, L-methionine, and L-cystine � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-416 17-104-CI 100 mL 6 15-30ºC 2-8ºC 18 Months

RPMI 1640, powder � With L-glutamine, without sodium bicarbonate � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-602 50-020-PB 10L 1 15-30ºC 2-8ºC 36 Months

45000-604 50-020-PC 50L 1 15-30ºC 2-8ºC 36 Months

B U F F E R E D S A L T S O L U T I O N SBuffered Salt Solutions

23

Buffered Salt Solutions

Dulbecco’s Phosphate Buffered Saline (DPBS) 25Phosphate Buffered Saline (PBS) 26Hanks’ Balanced Salt Solution (HBSS) 26

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24 Visit www.vwr.com for additional product and technical information.

Buffered Salt SolutionsIntroductionPhysiochemical PropertiesBasal salt solutions undergo testing to determine pH and osmolality according to specific protocols which are in line with the National Institute of Standards and Technology.

Biological TestsCorning cellgro prides itself on the ability to guarantee low levels of endotoxin, the absence of mycoplasma, and an absence of contamination.

Endotoxin: Buffered salt solutions are tested for bacterial endotoxin using the Limulus Amoe-bocyte Lysate (LAL) Assay, following guidelines outlined by the manufacturer and the FDA (1987). For release, each lot of buffered salt solutions must have an endotoxin level of ≤ 0.25 EU/mL. The majority of Corning cellgro’s liquid media are at or below our lowest detect-able level today (≤ 0.001 EU/mL). Powder products must have an endotoxin level of ≤ 0.25 EU/mL when reconstituted to formula weight with low-endotoxin water.

Sterility: Corning cellgro selects a number of samples from each production lot to test using the membrane filtration technique for 14 days, in accordance with USP XXIV Chapters <71> and <1211>.

Mycoplasma: Buffered salt solution products are tested for mycoplasma off-site using large-volume method described by Kern and Barile in “Isolation of Mycoplasmas from Cell Culture by Agar and Broth Techniques,” M.F. Barile and G.J. McGarrity (1983) in Methods of Mycoplasmology, Vol. 11,5. Razin and J.G. Tulley, eds., New York: Academic Press, 159-165.

Dulbecco’s Phosphate Buffered Saline (DPBS)Dulbecco’s Phosphate Buffered Saline (DPBS) is a buffer solution which can be used to maintain cell culture media in the physiological pH range of 7.0 to 7.6. It can serve as an irrigating, transporting or diluting fluid while maintaining cell tonicity and viability for a limited period of time.

Contains 45000-426 45000-42845000-43245000-430

45000-43645000-43445000-44045000-438

45000-44845000-446 45001-064 45000-442

Calcium ¢ ¢

Magnesium ¢ ¢

Potassium Chloride ¢ ¢ ¢ ¢ ¢ ¢


25

Dulbecco’s Phosphate Buffered Saline (DPBS)

Dulbecco’s Phosphate Buffered Saline, 10x � With calcium and magnesium � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-426 20-030-CV 500 mL 6 15-30ºC 2-30ºC 18 Months

Dulbecco’s Phosphate Buffered Saline, 10x � Without calcium and magnesium � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-428 20-031-CV 500 mL 6 15-30ºC 2-30ºC 18 Months

Dulbecco’s Phosphate Buffered Saline, 1x � With calcium and magnesium � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-430 21-030-CV 500 mL 6 15-30ºC 2-30ºC 36 Months

45000-432 21-030-CM 1L 6 15-30ºC 2-30ºC 36 Months

Dulbecco’s Phosphate Buffered Saline, 1x � Without calcium and magnesium � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-434 21-031-CV 500 mL 6 15-30ºC 2-30ºC 36 Months

45000-436 21-031-CM 1L 6 15-30ºC 2-30ºC 36 Months

45000-438 21-031-LX 10L 1 15-30ºC 2-30ºC 36 Months

45000-440 21-031-LB 20L 1 15-30ºC 2-30ºC 36 Months

Dulbecco’s Phosphate Buffered Saline, Powder � Without calcium and magnesium � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-442 55-031-PB 10L 1 15-30ºC 2-8ºC 36 Months

45000-444 55-031-PC 50L 1 15-30ºC 2-8ºC 36 Months


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Phosphate Buffered Saline (PBS)Phosphate buffered saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate, and in some formulations potassium chloride and potassium phosphate. PBS helps to maintain a constant pH and is isotonic and non-toxic to cells.

Phosphate Buffered Saline, 1x � Without calcium and magnesium pH 7.4 ± 0.1 � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-446 21-040-CV 500 mL 6 15-30ºC 2-30ºC 24 Months

45000-448 21-040-CM 1L 1 15-30ºC 2-30ºC 24 Months

Phosphate Buffered Saline, 10x � Without calcium and magnesium pH 7.4 ± 0.1 � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45001-130 46-013-CM 1L 6 15-30ºC 15-30ºC 24 Months

Hanks’ Balanced Salt Solution (HBSS)Hanks’ Balanced Salt Solution (HBSS) is a buffer used to maintain a physiological pH for cells maintained in non-CO2 atmospheric conditions.

Contains 45000-450 45001-15245000-45445000-452

45000-46045000-458

45000-46445000-462

45001-10145000-456 45000-470 45000-466

Calcium ¢ ¢ ¢

Magnesium ¢ ¢ ¢


Sodium Bicarbonate

¢ ¢ ¢ ¢

Hanks’ Balanced Salt Solution, 10x � Without sodium bicarbonate, calcium, and magnesium � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-450 20-021-CV 500 mL 6 15-30ºC 2-30ºC 36 Months

Hanks’ Balanced Salt Solution, 10x � With calcium and magnesium, without phenol red and sodium bicarbonate � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45001-152 20-023-CV 500 mL 6 15-30ºC 2-30ºC 36 Months


27

Hanks’ Balanced Salt Solution, 1x � With calcium and magnesium � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-452 21-020-CV 500 mL 6 15-30ºC 2-30ºC 36 Months

45000-454 21-020-CM 1L 6 15-30ºC 2-30ºC 36 Months

Hanks’ Balanced Salt Solution, 1x � With calcium and magnesium � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-456 21-023-CV 500 mL 6 15-30ºC 2-30ºC 36 Months

45001-101 21-023-CV 1L 6 15-30ºC 2-30ºC 36 Months

Hanks’ Balanced Salt Solution, 1x � Without calcium and magnesium � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-458 21-021-CV 500 mL 6 15-30ºC 2-30ºC 36 Months

45000-460 21-021-CM 1L 6 15-30ºC 2-30ºC 36 Months

Hanks’ Balanced Salt Solution, 1x � Without calcium, magnesium, and phenol red � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-462 21-022-CV 500 mL 6 15-30ºC 2-30ºC 36 Months

45000-464 21-022-CM 1L 6 15-30ºC 2-30ºC 36 Months

Hanks’ Balanced Salt Solution, Powder � Without sodium bicarbonate, calcium and magnesium � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-466 55-022-PB 10L 1 15-30ºC 2-8ºC 36 Months

Hanks’ Balanced Salt Solution, Powder � Without sodium bicarbonate, calcium, magnesium, and phenol red � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-470 55-021-PB 10L 1 15-30ºC 2-8ºC 36 Months


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DPBS, PBS, and TBS Formulation

VWR Cat. No. 45000-426 45000-428 45000-430

45000-43645000-43445000-44045000-438

45000-44245000-444

45000-44845000-446 45001-130 45001-132

Description10x,

Liquid DPBS

10x, Liquid DPBS

1x, Liquid DPBS

1x, Liquid DPBS

PowderDPBS

1x, Liquid PBS

10x, Liquid PBS

10x, Liquid TBS

Volume g/L g/L g/L g/L g/L g/L g/L g/L

Inorganic Salts

CaCl2 (anhydrous) 1.00 - 0.10 - - - - -

KCl 2.00 2.00 0.20 0.20 0.20 - 2.0 -

KH2PO4 2.00 2.00 0.20 0.20 0.20 0.144 2.40 -

MgCl2 • 6H2O 1.00 - 0.10 - - - - -

NaCl 80.00 80.00 8.00 8.00 8.00 9.00 80.00 80.00

Na2HPO4 (anhydrous) 11.50 11.50 - 1.15 1.15 0.795 14.40 -

Na2HPO4 • 7H2O - - 2.1716 - - - - -

Tris Ultrapure - - - - - - - 24.2

Specifications

pH (before adjustment) - - - - 7.4 ± 0.1 - - -

Final pH 4.4 ± 0.3 6.5 ± 0.5 7.0 ± 0.1 7.4 ± 0.1 - 7.4 ± 0.1 7.4 ± 0.1 @ 1:10

24 - 26ºC @ 1:10

Osmolality (mOsm) 276 ± 30 @ 1:10

276 ± 30 @ 1:10 285 ± 15 276 ± 10 282 ± 15 300 ± 20 - -

Grams of powder requiredto prepare 1L (1x solution) - - - - 9.55 - - -


29

Hank’s Balanced Salt Solution (HBSS) Formulation

VWR Cat. No.

45000-46045000-458 45001-152

45000-45445000-452

45000-46045000-458

45000-46445000-462

45001-10145000-456 45000-470 45000-466

Description10x, Liquid

HBSS

10x, Liquid HBSS

1x, Liquid HBSS

1x, Liquid HBSS

1x, Liquid HBSS

1x, Liquid HBSS

Powder HBSS

Powder HBSS

Volume g/L g/L g/L g/L g/L g/L g/L g/L

Inorganic Salts

CaCl2 (anhydrous) - 1.4 0.14 - - 0.14 - -

KCl 4.00 4.00 0.40 0.40 0.40 0.40 0.40 0.40

KH2PO4 0.60 0.60 0.06 0.06 0.06 0.06 0.06 0.06

MgSO4 (anhydrous) - 0.977 0.0977 - - 0.0977 - -

NaCl 80.00 80.00 8.00 8.00 8.00 8.00 8.00 8.00

Na2HPO4 (anhydrous) 0.477 0.477 0.0477 0.0477 0.0477 0.0477 0.0477 0.0477

NaHCO3 - - 0.35 0.35 0.35 0.35 - -

Other

D-Glucose 10.00 10.00 1.00 1.00 1.00 1.00 1.00 1.00

Phenol red, Na 0.10 - 0.01 0.01 - - 0.01 -

Add

NaHCO3

Powder (g/L) 0.35 - - - - - 0.35 0.35

7.5% solution (mL/L) 4.67 - - - - - 4.67 4.67

Specifications

pH (before adjustment) - - - - - - - 6.6 ± 0.5

Final pH 6.0 ± 1.0 4.75 ± 0.25 7.25 ± 0.15 7.2 ± 0.2 7.25 ± 0.15 7.25 ± 0.15 7.25 ± 0.15 7.2 ± 0.2

Osmolality (mOsm/kg)275 ± 30

(1:10)275 ± 30

(1:10)275 ± 15 275 ± 15 271 ± 15 287 ± 15 282 ± 15 281 ± 15

Grams of powder required to prepare 1L (1x solution)

- - - - - - 9.52 9.51

S E R U M - F R E E A N D S P E C I A L T Y M E D I A

Serum-Free and

Specialty Media

31

Serum-Free and Speciality Media

cellgro COMPLETE™ 32insectagro DS2™ 33insectagro™ 33insectagro Sf9™ 33transfectagro™ 33Lymphocyte Separation Medium 34Polysucrose 400 34Tryptic Soy Broth (TSB) 35cellgro® rhAlbumin 35cellgro® rhLactoferrin 36stemgro® AB2™ Neural Base Media 36stemgro® ABS™ Neural Supplement 36


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Serum-Free and Speciality Media

cellgro COMPLETE™ Serum-Free/Low-Protein Medium

cellgro COMPLETE formulation is based on a proprietary combination of 1:1 DMEM/F12, RPMI and McCoy’s 5A medium. This medium contains a mixture of selected trace elements and high molecular weight carbohydrates, extra vitamins, animal origin-free protein, and a small amount of high quality bovine serum albumin (1.0 g/L). cellgro COMPLETE sup-ports superior growth and viability over long-term passages in hybridomas, along as suspen-sion and adherent cell cultures. This formulation contains L-glutamine.

cellgro COMPLETE Serum-Free/Low-Protein Medium, 1x � With L-glutamine and 1 g/L BSA � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-420 40-101-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

cellgro COMPLETE Serum-Free/Low-Protein Medium, 1x � With L-glutamine and 1 g/L BSA, without phenol red � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-422 40-102-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

cellgro FREE™ Serum-Free/Low-Protein MediumOptimized for the cultivation of Chinese Hamster Ovary (CHO) cells, along with many derivatives of this parent line, cellgro FREE is a proprietary serum-free and protein-free growth medium that does not contain any hormones or growth factors. This medium sup-ports superior growth and viability over long term passages in both adherent and suspension culture, and is formulated without L-glutamine.

cellgro FREE Serum-Free/Protein-Free Medium, 1x � Without L-glutamine � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-424 40-200-CV 500 mL 6 15-30ºC 2-8ºC 12 Months


Serum-Free and

Specialty Media

33

insectagro DS2™ Serum-Free/Protein-Free Medium

insectagro DS2 Serum-Free/Protein-Free Medium was developed for the growth and main-tenance of Drosophila Schneider 2 (S2) cells to be used in heterologous protein expression. At the optimal temperature range 22-24ºC, DS2 cells grow as a loose monolayer and are readily adaptable to growth in suspension. Under these conditions, the cells require minimal adaptation to serum-free culture.

insectagro DS2 Serum-Free/Protein-Free Medium � Without L-glutamine � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-474 13-402-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

insectagro Sf9™ Serum-Free/Protein-Free Mediuminsectagro Sf9 is formulated to support the propagation of Sf9 insect cells in culture, and can also be used for SF21 cells. Sf9 cells are cultured in non-humidified, non-CO2 incubators at 27ºC (room temperature) and display both monolayer and suspension culture qualities. With their fast doubling times of 10-22 hours, Sf9 cells are easily scaled up to large cultures using bioreactors. This formulation contains L-glutamine.

insectagro Sf9 Serum-Free/Protein-Free Medium � With L-glutamine � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-054 13-410-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

transfectagro™transfectagro reduced serum medium is designed to maximize the growth of a variety of cell types under reduced serum conditions, generally allowing for at least a 50% reduction in serum use with minimal adaptation. transfectagro also improves transient transfection efficiency in a variety of cell types. This is a chemically-defined, reduced serum formulation.

transfectagro Reduced Serum Medium � With lower levels of calcium and phenol red, and with HEPES � Intended Use: In Vitro Diagnostic

VWR Cat. No.


71002-816 40-300-CV 500 mL 6 15-30ºC 2-8ºC 12 MonthsNew


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Lymphocyte Separation MediumLymphocyte Separation Medium (LSM) was originally designed for the In Vitro isolation of lymphocytes from diluted whole blood. It is a sterile filtered, iso-osmotic polysucrose and diatrizoate solution with low viscosity and a density of 1.077-1.080 g/mL at 20ºC.

Reagents: Lymphocyte Separation Medium is a sterile filtered solution containing 96.219 g/L of diatrizoic acid and 61.363 g/L polysucrose 400 at a density of 1.077 - 1.080 g/mL @ 20ºC. The solution has an osmolarity of 290 ± 20 mOsm and a pH of 7.5 ± 1.5. Sodium hydroxide is added to adjust pH.

Lymphocyte Separation Medium � Density - 1.077 - 1.080 g/mL at 20ºC � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-726 25-072-CI 100 mL 1 15-30ºC 15-30ºC 18 Months

45000-728 25-072-CV 500 mL 1 15-30ºC 15-30ºC 18 Months

Polysucrose 400Polysucrose 400 is a synthetic polymer of sucrose and epichlorohydrin used for the density gradient separation of cells, cell membranes and virus cells. It can also be used to isolate different organelles. This nonionic compound is provided in powder form.

Polysucrose 400, Powder � Used for cell separation � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-730 61-196-RM 100 g 1 15-30ºC 15-30ºC ≥ 12 Months

45000-732 61-196-RO 500 g 1 15-30ºC 15-30ºC ≥ 12 Months


Serum-Free and

Specialty Media

35

Tryptic Soy Broth (TSB)Tryptic Soy Broth (TSB) is offered for use in sterility testing and validation in the manufac-ture of a liquid product. The use of this medium in place of the product intended for produc-tion allows an accurate assessment of sterility throughout the manufacture process. It is a sterile filtered, ready-to-use solution for the imulation of aseptic filling of liquids and sterility testing. Due to the rich nutrient base, TSB may be used in the cultivation of fastidious mi-croorganisms. This growth medium complies with current USP requirements and is free of animal-sourced components, making it ideal for testing the sterility of animal-free biophar-maceutical processes.

Tryptic Soy Broth, Animal-Free, Liquid � pH 7.1 - 7.5 @ 20-25ºC � Tested negative for mycoplasma � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-156 46-060-CI 100 mL 1 15-30ºC 15-30ºC 12 Months

Tryptic Soy Broth, Animal-Free, Powder � Used in the cultivation of fastidious microorganisms. � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-226 61-411-RO 500 g 1 15-30ºC 15-30ºC ≥ 12 Months

cellgro® rhAlbumincellgro rhAlbumin is a chemically-defined recombinant human serum albumin (rhHSA). Having an essential and non-essential fatty acid profile, this is a powerful media supplement that outperforms plasma-derived human serum albumin (pHSA), bovine serum albumin (BSA) and FBS. Some prominent applications for cellgro rhAlbumin are as a supplement for CHO cell culture and for the culture of various other cell lines. This product is plant-derived and completely animal-origin free.

cellgro rhAlbumin � Recombinant human serum albumin � Intended Use: Research Use Only

VWR Cat. No.


89133-078 62-450-RF 1 g 1 15-30ºC -20-8ºC 12 Months

Long Term-20ºC


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cellgro® rhLactoferrincellgro rhLactoferrin is a recombinant human hololactoferrin specifically intended for use as a cell culture supplement. It is a multi-functional protein that is a potent cell growth factor with anti-apoptotic and anti-microbial properties. This product is plant-derived and com-pletely animal-origin free.

Corning cellgro rhLactoferrin, Powder � Recombinant human hololactoferrin � Intended Use: Research Use Only

VWR Cat. No.


89133-080 61-451-RF 1 g 1 15-30ºC -20- 8ºC N/A

stemgro® AB2™ Neural Base Mediastemgro AB2 Neural Base Media is designed for use with stemgro® ANS™ Neural Supple-ment. The main applications are the expansion and proliferation Neural Progenitor Cells, and the maintenance of Differentiated Neuronal Cells. This basal medium is free of animal and protein components.

stemgro AB2 Neural Base Media

VWR Cat. No.


97060-260 40-400-CV 500 mL 1 Ambient 2-8ºC 18 Months

stemgro® ANS™ Neural Supplementstemgro ANS Neural Supplement is used with stemgro AB2 Neural Base Media as part of a complete formulation for the expansion and proliferation of Neural Progenitor Cells, and for the maintenance of Differentiated Neuronal Cells.

stemgro ABS Neural Base Media

VWR Cat. No.


97060-262 25-040-CR 2 mL 5 -25-5ºC -25-5ºC 18 Months

H U M A N A N D A N I M A L S E R AH

uman and Anim

al Sera

39

Human and Animal Sera

Fetal Bovine Serum 43Donor Horse Serum 45Human AB Serum 45Porcine Serum 45Charcoal-Stripped FBS 46Dialyzed FBS 46ES Cell Tested FBS 46Gamma-Irradiated FBS 47Low IgG FBS 47Tetracycline Negative FBS 47


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Human and Animal Sera

Introduction

Our high-quality serum products complement our wide range of sterile filtered, low endo-toxin classical cell culture media perfectly:

Fetal Bovine Serum (FBS) is a sterile, light brown colored liquid separated from the blood of the bovine fetus. All material meets the approval of the U.S. Department of Agriculture and is obtained from sources free of BSE, Foot and Mouth Disease, and other reportable diseases pertaining to the species.

U.S. Sourced: Our U.S. sourced Premium Fetal Bovine serum, with the lowest endotoxin and hemoglobin specifications, is collected from cattle herds only within the United States. Also available heat-inactivated.

USDA: The USDA-certified Fetal Bovine Serum, also low in endotoxin and hemoglobin, is derived from USDA-certified facilities outside the United States, primarily Mexico. Mexico is recognized by the United States Department of Agriculture (USDA) to be free of bovine spongiform encephalopathy (BSE) and Foot and Mouth Disease (FMD). This is also available heat-inactivated.

Donor Calf Serum is a sterile, light brown-colored liquid collected from herds of bovine calves located entirely in the United States. Animals are regularly inspected by licensed veterinarians and certified to be free of diseases pertaining to the species.

Donor Horse Serum is a sterile, light brown-colored liquid collected from controlled standing herds of animals located entirely in the United States. Animals are inspected periodically by a licensed veterinarian. Diet and worming are strictly controlled and documented to ensure all animals are healthy. Each lot of Donor Horse Serum is extensively tested, and must be negative for Equine Infectious Anemia (EIA) and Equine Viral Arteritis (EVA). Chemical constituents are measured further to ensure lot-to-lot consistency.

Porcine Serum is a sterile, light-brown liquid, collected from healthy pigs of U.S.-Origin, and free of Foot and Mouth Disease, Rinderpest, and Bovine Spongiform Encephalopathy. Each lot undergoes a biochemical analysis to ensure consistency.

Specialty Serum products are also offered by Corning cellgro for a variety of product applications.

All sera is separated from the collected blood by refrigerated centrifugation, true-pooled, filtered, and immediately frozen. The filtration process consists of a series of three 0.1 micron sterilizing filters, followed by a final “polishing” filter prior to final packaging. Only raw serum that meets our strict specifications for endotoxin, residual hemoglobin, pH and microbial quality is approved for production.


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Serum Product SpecificationsFetal Bovine Serum, Premium

VWR Cat. No.

Size Qty/Pk

45001-106 500 mL 1

45001-108 500 mL 1

Endotoxin ≤ 20 EU/mL

Hemoglobin ≤ 30 mg/dL

Total Protein 40 ± 10 g/L

pH 7.8 ± 0.8

Osmolality 315 ± 35 mOsm/kg

Sterility Testing (current USP) Sterile

Virus Testing [9CFR 113.53]

Mycoplasma Negative

Fetal Bovine Serum, Regular

VWR Cat. No.

Size Qty/Pk

45000-734 500 mL 1

45000-736 500 mL 1

Endotoxin ≤ 20 EU/mL


Total Protein 40 ± 10 g/L

pH 7.6 ± 0.8




Mycoplasma Negative

Donor Calf Serum

VWR Cat. No.

Size Qty/Pk

45001-060 500 mL 1

Endotoxin ≤ 5.0 EU/mL


Total Protein 6.0 ± 2.0 g/dL

pH 7.8 ± 0.8




Mycoplasma Negative


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Serum Product SpecificationsDonor Horse Serum

VWR Cat. No.

Size Qty/Pk

45001-058 500 mL 1

Endotoxin (LAL) ≤ 5.0 EU/mL



pH 7.8 ± 0.8




Mycoplasma Negative

Human AB Serum

VWR Cat. No.

Size Qty/Pk

45001-062 100 mL 1

Endotoxin (LAL) ≤ 5.0 EU/mL



pH 7.8 ± 0.8




Mycoplasma Negative


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Fetal Bovine SerumFetal Bovine Serum (FBS) is the most common supplement used in cell culture. Its use at specific concentrations provides many compounds that have been shown to satisfy specific metabolic requirements for the culture of cells, including various growth promoting factors, along with a variety of undefined components.

Fetal Bovine Serum, Premium � Hemoglobin < 30 mg/dL Endotoxin ≤ 20 EU/mL � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.

Corning Cat. No. Size Qty/Pk Shipping Storage

45001-106 35-015-CV 500 mL 1 -15-25ºC -15-25ºC

Fetal Bovine Serum, Premium (Heat Inactivated) � Hemoglobin < 30 mg/dL Endotoxin ≤ 20 EU/mL � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-108 35-016-CV 500 mL 1 -15-25ºC -15-25ºC

Gamma-Irradiated Fetal Bovine Serum, Premium � Used in virus and vaccine production, projects needing a minimized risk of virus and mycoplasma contamination along with very low endotoxin.

� Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


97065-300 35-070-CV 500 mL 1 -15-25ºC -15-25ºC

Dialyzed Fetal Bovine Serum, Premium � Used in any application needing a more defined serum or a need for low endotoxin concentration.


VWR Cat. No.


97065-302 35-071-CV 500 mL 1 -15-25ºC -15-25ºC

Charcoal-Stripped Fetal Bovine Serum, Premium � Used in immunoassays, hormone-receptor assays, low virus/endotoxin applications. � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


97065-304 35-072-CV 500 mL 1 -15-25ºC -15-25ºC


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Fetal Bovine SerumFetal Bovine Serum, Regular

� Hemoglobin < 30 mg/dL Endotoxin ≤ 20 EU/mL � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.

Corning Cat. No.

Size Qty/Pk Shipping Storage

45000-734 35-010-CV 500 mL 1 -15-25ºC -15-25ºC

Fetal Bovine Serum, Regular (Heat Inactivated) � Hemoglobin < 30 mg/dL Endotoxin ≤ 20 EU/mL � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-736 35-011-CV 500 mL 1 -15-25ºC -15-25ºC

IgG Stripped Fetal Bovine Serum, Premium � Used in commercial antibody production, specialized organism propagation. � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


97065-306 35-073-CV 500 mL 1 -15-25ºC -15-25ºC

Embryonic Stem Cell Tested FBS, Premium � Used in embryonic stem cell research (validated for mouse but suitable for human) � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


97065-308 35-074-CV 500 mL 1 -15-25ºC -15-25ºC

Tetracycline Negative Fetal Bovine Serum, Premium � Used in cell culture applications using tet-inducible gene expression systems, any other application in which the presence of tetracycline would prove disruptive.


VWR Cat. No.


97065-310 35-075-CV 500 mL 1 -15-25ºC -15-25ºC


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Donor Calf Serum, U.S. SourcedDonor Calf Serum is collected from bovine calves less than eight months of age. The serum is separated from the blood by a mechanical defibrination process. Donor calf serum can be used for cells which have shown especially rapid growth with fetal bovine serum but is not the best choice for suspension cultures because of the turbidity associated with its use, which could complicate cell counting and viability measurements.

Donor Calf Serum, U.S. Sourced � Hemoglobin ≤ 20 mg/dL Endotoxin ≤ 5.0 EU/mL � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-060 35-022-CV 500 mL 1 -15-25ºC -15-25ºC up to 5 years

Donor Horse Serum, U.S. SourcedDonor Horse Serum is serum collected aseptically from healthy equines. The serum is separated aseptically from the blood of the animal and allowed to spontaneously clot. Horse serum can be combined with fetal bovine serum for the culture of rat adrenal pheochromocytoma (PC12) cells, and is often used in testing media for mycoplasma contamination.

Donor Horse Serum, U.S. Sourced � Hemoglobin ≤ 20 mg/dL Endotoxin ≤ 5.0 EU/mL � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-058 35-030-CV 500 mL 1 -15-25ºC -15-25ºC

Human AB SerumHuman AB Serum is processed from donor plasma collected at U.S. centers. Each donor unit of plasma is negative for Hepatitis B (HBsAg), Hepatitis C (anti-HCV), Human Immu-nodeficiency Virus-1 (HIV-1), Human Immunodeficiency Virus-2 (HIV-2), Human Immuno-deficiency Virus antibody (HIV-1Ag), and ALT by FDA approved methods.

Note: Proper safety precautions for potentially infectious agents must be taken despite these assurances.

Human AB Serum � Hemoglobin ≤ 20 mg/dL Endotoxin ≤ 5.0 EU/mL � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-062 35-060-CI 100 mL 1 -15-25ºC -15-25ºC 24 Months


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Specialized Serum

Charcoal-Stripped FBSCharcoal-Stripped FBS has been subjected to charcoal filtration, which removes liphilic compounds (hormones, certain growth factors and cytokines) and virus particles. Charcoal-Stripped FBS is often used in immunoassays and hormone studies.

� Used in immunoassays and hormone studies. Charcoal or charcoal-dextran removes lipophilic compounds (hormones, certain growth factors and cytokines) and virus particles.


VWR Cat. No.


97065-304 35-072-CV 500 mL 1 -15-25ºC -15-25ºC

Dialyzed FBSFetal bovine serum contains a wide variety of biological molecules and compounds which can influence cell development. This serum is dialyzed against physiological saline to remove small (less than 10kDa) potential contaminants while preserving the biological integrity of the final product. An extra filtration step of 0.1 micron further ensures purity. Dialyzed FBS is ideal for any application needing a more defined serum or a need for low endotoxin concentration.

� Used in any application needing a more defined serum or a need for low endotoxin concentration.


VWR Cat. No.


97065-302 35-071-CV 500 mL 1 -15-25ºC -15-25ºC 36 Months

ES Cell Tested FBS, U.S. OriginES Cell Tested FBS meets the specialized needs of Embryonic Stem Cells (ESC’s). These cells require a strictly controlled environment to minimize growth factor and cytokine-incuded differentiation. Embryonic stem cell qualified FBS is stringently validated for its ability to support mouse ESC colony formulation and proper cell morphology while allowing for minimal differentiation.

� Used in embryonic stem cell research (validated for mouse but suitable for human). � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


97065-300 35-070-CV 500 mL 1 -15-25ºC -15-25ºC


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Gamma-Irradiated FBS

Gamma irradiation provides a method for complete viral and mycoplasma deactivation while maintaining the biological activity of the final product. Gamma-irradiated serum is useful for projects requiring very low endotoxin and a minimized risk of virus and mycoplasma contamination.

� Used in virus and vaccine production, projects needing a minimized risk of virus and mycoplasma contamination along with very low endotoxin.


VWR Cat. No.


97065-300 35-070-CV 500 mL 1 -15-25ºC -15-25ºC

Low IgG FBSLow IgG FBS has been tested for the presence of IgG antibodies to meet strict specifications. It is useful in IgG antibody production, in the propagation of organisms that are normally hindered by the presence of IgGs, and any project in which downstream results would be hindered by exogenous IgGs.

� Used in commercial antibody production, specialized organism propagation. � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


97065-306 35-073-CV 500 mL 1 -15-25ºC -15-25ºC

Tetracycline Negative FBS, U.S. OriginTetracycline Negative FBS is tested for the presence of tetracycline, an antibiotic that is frequently found in sera. Researchers using very sensitive tet-inducible gene expression systems encounter problems with unintended expression as a result of the presence of tetracycline in serum. In these cases, serum containing minimal levels of tetracycline can help ensure accurate gene expression. The tetracycline test is preformed either with the ELISA method or bioassay methodology techniques. The limit of detection is approximately 0.1 µg/ml.

� Used in cell culture applications using tet-inducible gene expression systems, any other application in which the presence of tetracycline would prove disruptive.


VWR Cat. No.


97065-310 35-075-CV 500 mL 1 -15-25ºC -15-25ºC

C E L L C U L T U R E R E A G E N T SCell Culture Reagents

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Cell Culture Reagents

L-glutamine 51glutagro™ 51MEM Amino Acids 52MEM Vitamins 52Antibiotics and Antimycotics 52Buffers 56Trypsin 57Non-Enzymatic Cell Dissociation Agents 58Growth Supplements 58Hybridoma Reagents 58Miscellaneous Reagents 59

C E L L C U L T U R E R E A G E N T SCe

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Cell Culture Reagents

IntroductionGrowth media is used either for cell culture or microbiological culture. These require nutrients, supplements, buffers and other reagents specific to the product being grown to maximize results and consistency.

There are two major types of growth media: those used for cell culture, which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast.

The most common growth media for microorganisms are nutrient broths and agar plates; specialized media are sometimes required for microorganism and cell culture growth. Some organisms, termed fastidious organisms, require specialized environments due to complex nutritional requirements. Viruses, for example, are obligate intracellularparasites and require a growth medium composed of living cells.


51

Amino Acids and Vitamins

L-glutamineL-glutamine is an essential amino acid and a key component of culture media, serving as a major energy source for propagating cells. It is very stable as a dry powder and as a frozen solution but degrades rapidly in liquid media or stock solutions, producing compounds toxic to cells. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use. This formulation is prepared in cell culture grade water.

L-glutamine, Liquid � 200 mM solution (29.20 mg/mL with 8.5 g/L NaCl) � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-676 25-005-CI 100 mL 6 -15-25ºC -15-25ºC 24 Months

45000-678 25-005-CV 500 mL 6 -15-25ºC -15-25ºC 24 Months

L-glutamine, Powder � Molecular weight 146.15 � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-680 61-030-RM 100 g 1 15-30ºC 2-8ºC 12 Months

45000-682 61-030-RO 500 g 1 15-30ºC 2-8C 12 Months

45000-684 61-030-RR 1 kg 1 15-30ºC 2-8C 12 Months

glutagro™L-glutamine is an essential amino acid and a key component of culture media, serving as a major energy source for propagating cells. It is very stable as a dry powder and as frozen solution but degrades rapidly in liquid media or stock solutions, producing toxin compounds. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use. glutagro is a stabilized dipeptide form of L-glutamine which does not degrade in liquid media during storage or incubation, providing superior results in several application.

glutagro (Dipeptide L-glutamine), Liquid � 200 mM solution with 8.5 g/L NaCl � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45001-086 25-015-CI 100 mL 1 15-30ºC 2-30ºC 24 Months

AMEMBMEDMEMDMEM/F12EMEMIsocove’s DMEMM199RPMI 1640

292 mg/L292 mg/L584 mg/L365.10 mg/L292 mg/L584 mg/L100 mg/L292 mg/L

Supplementation table for L-Glutamine and L-Alanyl-L-Glutamine to L-Glutamine-Deficient Culture Media

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MEM Amino Acids

MEM Amino Acids � 50x solution without L-glutamine � Allows for efficient addition to media with no risk of dilution of media components � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-698 25-030-CI 100 mL 6 15-30ºC 2-8ºC 36 Months

MEM Nonessential Amino Acids � 100x solution � The addition of NEAA to medium can improve growth and viability of propagating cells in culture while reducing the biosynthetic burden on these cells


VWR Cat. No.


45000-700 25-025-CI 100 mL 6 15-30ºC 2-8ºC 18 Months

MEM Vitamins

MEM Vitamins � 100x solution � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-702 25-020-CI 100 mL 6 -15-25ºC -15-25ºC 24 Months

Antibiotics and Antimycotics

Amphotericin B, Liquid � 250 µg/mL solubilized � Intended Use: Research Use Only

VWR Cat. No.


45000-808 30-003-CF 50 mL 6 -15-25ºC -15-25ºC 18 Months

Ampicillin, Sodium Salt � Inhibits bacterial cell-wall synthesis � Intended Use: Research Use Only

VWR Cat. No.


45000-612 61-238-RH 10 g 1 15-30ºC 2-8ºC ≥ 12 Months

45000-614 61-238-RM 100 g 1 15-30ºC 2-8ºC ≥ 12 Months


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Antibiotic-Antimycotic Solution � 10,000 I.U. Penicillin (per mL), 10,000 µg/mL Streptomycin, 25µg/mL Amphotericin with 8.5 g/L NaCl


VWR Cat. No.


45000-616 30-004-CI 100 mL 6 -15-25ºC -15-25ºC 18 Months

Blasticidin S HCl, Powder � Inhibits translation in prokaryotes and eukaryotes � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


71002-676 30-100-RB 100 mL 6 -15-25ºC -15-25ºC 18 Months

Chloramphenicol, Powder � Prototypical broad spectrum antibiotic � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-618 61-239-RI 25 g 1 15-30ºC 15-30ºC ≥ 12 Months

Ciprofloxacin Hydrochloride, Powder � A fluoroquinolone antibiotic � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-620 61-277-RG 1 g 1 15-30ºC 15-30ºC ≥ 12 Months45000-622 61-277-RG 5 g 1 15-30ºC 15-30ºC ≥ 12 Months

G418 Sulfate, Liquid � 50 mg/mL solution � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-630 30-234-CR 20 mL 1 15-30ºC 2-8ºC ≥ 12 Months45001-076 30-234-CI 100 mL 1 15-30ºC 2-8ºC ≥ 12 Months


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G418 Sulfate, Powder � Potency > 700 µg/mg � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-624 61-234-RF 1 g 1 15-30ºC 15-30ºC ≥ 12 Months45000-626 61-234-RG 5 g 1 15-30ºC 15-30ºC ≥ 12 Months45000-628 61-234-RK 50 g 1 15-30ºC 15-30ºC ≥ 12 Months

Gentamicin Sulfate, Liquid � Broad spectrum antibiotic recommended at 50 µg/mL � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-636 30-005-CR 10 mL 10 15-30ºC 15-30ºC 12 Months

Gentamicin Sulfate, Powder � Broad spectrum antibiotic recommended at 50 µg/mL � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-632 61-098-RA 100 mg 1 15-30ºC 2-8ºC ≥ 12 Months45000-634 61-098-RF 1 g 1 15-30ºC 2-8ºC ≥ 12 Months

Hygromycin B Solution � Blocks polypeptide synthesis and inhibits elongation � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-806 30-240-CR 20 mL 1 15-30ºC 2-8ºC 18 Months

Kanamycin Sulfate, Liquid � Inhibits protein synthesis � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-810 30-006-CF 50 mL 6 -15-25ºC -15-25ºC 18 Months

Kanamycin Sulfate, Powder � Inhibits protein synthesis � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-640 61-176-RG 5 g 1 15-30ºC 15-30ºC 18 Months


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Neomycin Sulfate, Powder � Inhibits initiation and elongation during protein synthesis � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-644 61-241-RG 5 g 1 15-30ºC 15-30ºC 18 Months

Penicillin-Streptomycin Solution, 50x � 5,000 I.U. penicillin (per mL), 5,000 µg/mL streptomycin � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-650 30-001-CI 100 mL 6 -15-25ºC -15-25ºC 18 Months

Penicillin-Streptomycin Solution, 100x � 10,000 I.U. penicillin (per mL), 10,000 µg/mL streptomycin � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-652 30-002-CI 100 mL 6 -15-25ºC -15-25ºC 12 Months

Penicillin-Streptomycin-L-Glutamine, 100x � 10,000 I.U. penicillin (per mL), 10,000 µg/mL streptomycin, 29.2 mg/mL L-glutamine � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-103 30-009-CI 100 mL 6 -15-25ºC -15-25ºC 12 Months

Puromycin Dihydrochloride, Powder � A broad-spectrum aminonucleoside antibiotic � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


62111-170 61-385-RA 100 mL 1 -15-25ºC -15-25ºC 12 Months

Streptomycin Sulfate, Powder � An aminoglycoside antibiotic � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-654 61-088-RM 5 g 1 2-8ºC 2-8ºC 12 Months

Tetracycline Hydrochloride, Powder � Broad spectrum antibiotic � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.

Corning Cat. No.


45000-656 61-242-RG 5 g 1 2-8ºC 2-8ºC 12 Months


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Buffers

HEPES, Liquid � 1M solution (238.3 mg/mL) � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-690 25-060-CI 100 mL 6 15-30ºC 15-30ºC 24 Months

HEPES, Powder � Molecular Weight 238.3 � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-692 61-034-RM 100 g 1 15-30ºC 15-30ºC ≥ 12 Months45000-694 61-034-RO 500 g 1 15-30ºC 15-30ºC ≥ 12 Months45000-696 61-034-RR 1 kg 1 15-30ºC 15-30ºC ≥ 12 Months

Sodium Bicarbonate, Liquid � Commonly used to maintain physiological pH of 7.2-7.4 � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-704 25-035-CI 100 mL 6 15-30ºC 15-30ºC 36 Months

Sodium Bicarbonate, Powder � Molecular Weight 84.01 � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-706 61-065-RO 500 g 1 15-30ºC 15-30ºC ≥ 12 Months45000-708 61-065-RR 1 kg 1 15-30ºC 15-30ºC ≥ 12 Months


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Trypsin

Trypsin, 1x � 0.25% trypsin in HBSS, without calcium and magnesium � Porcine parvovirus tested � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-658 25-050-CI 100 mL 6 -15-25ºC -15-25ºC 12 Months

Trypsin EDTA, 1x � 0.05% trypsin/0.53 mM EDTA in HBSS, with sodium bicarbonate, calcium, and magnesium � Porcine Parvovirus Tested � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45001-082 25-051-CI 100 mL 6 -15-25ºC -15-25ºC 12 Months

Trypsin EDTA, 1x � 0.05% trypsin/0.53 mM EDTA in HBSS, without sodium bicarbonate, calcium and magnesium � Porcine parvovirus tested � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45001-660 25-052-CI 100 mL 6 -15-25ºC -15-25ºC 12 Months

45001-662 25-052-CV 500 mL 6 -15-25ºC -15-25ºC 12 Months

Trypsin EDTA, 1x � 0.25% trypsin/2.21 mM EDTA in HBSS, without sodium bicarbonate, calcium, and magnesium � Porcine Parvovirus tested � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-664 25-053-CI 100 mL 6 -15-25ºC -15-25ºC 12 Months

Trypsin, 10x � 2.5% trypsin in HBSS without calcium, magnesium, and phenol red � Porcine parvovirus tested � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-666 25-054-CI 100 mL 6 -15-25ºC -15-25ºC 12 Months


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Non-Enzymatic Cell Dissociation Agents

Cellstripper™Cellstripper is a non-enzymatic cell dissociation solution formulated with a proprietary mixture of chelators that gently dislodge adherent cells in tissue culture. It is an alternative to trypsin when used with either serum-free or serum-supplemented media. Because Cellstripper is a gentler reagent than trypsin, cells can be exposed to Cellstripper for longer periods of time. Cellstripper achieves superior results in comparison to enzymatic dissociation products.

Cellstripper, Liquid � Non-enzymatic cell dissociation solution � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-668 25-056-CI 100 mL 6 15-30ºC 15-30ºC 12 Months

Growth Supplements

Insulin-Transferrin-Selenium (ITS), 100x � For use in adherent (anchorage-dependent) cultures � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45001-090 25-800-CR 10 mL 1 2-8ºC 2-8ºC 18 Months

Hybridoma Reagents

HAT (Hypoxanthine, Aminopterin, Thymidine), 50x

Hypoxanthine, aminopterin, and thymidine (HAT) media is used in mammalian cell culture for post-fusion selection to eliminate certain types of myeloma cells


VWR Cat. No.


45000-686 25-046-CI 100 mL 1 -15-25ºC -15-25ºC 28 Months

HT (Hypoxanthine, Thymidine). 50x

Hypoxanthine and thymidine (HT) media is a “rescue media” that provides preformed purines and pyridmidines to overcome the effects of residual intracellular aminopterin


VWR Cat. No.


45000-688 25-047-CI 100 mL 1 15-30ºC 2-8ºC 20 Months


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Miscellaneous Reagents

Dimethyl Sulfoxide (DMSO)

Dimethyl sulfoxide (DMSO) is commonly used in cell cryopreservation, along with glycerol, to prevent damage to the cell membrane during freezing.


VWR Cat. No.


45001-118 25-950-CQC 250 mL 1 15-30ºC 15-30ºC 12 Months

45% Glucose Solution

Glucose is a main source of energy for living organisms and is used as a nutrient source in cell culture media. This 45% (w/v) solution has been ensured no cytotoxicity.


VWR Cat. No.


45001-116 25-037-CI 100 mL 1 15-30ºC 15-30ºC 24 Months

Sodium PyruvateSodium pyruvate is used as a component in culture broth and media as an energy source for propagating cells. It is also used in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid. It is provided as a 100 mM solution with 8.5 g/L NaCl.

� 100 mM solution with 8.5 g/L NaCl � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-710 25-000-CI 100 mL 6 15-30ºC 2-8ºC 18 Months

Trace Elements

Trace Elements A (1,000x)

Trace Elements A provides copper, zinc, iron and selenium. Selenium aids in the detoxifica-tion of free radicals as a cofactor for GSH synthetase, while iron, copper, and zinc may be bound by serum protein. The combination of Trace Elements A contains the trace elements of MCDB 104 and of MCDB 301


VWR Cat. No.


45000-712 25-021-CI 100 mL 1 15-30ºC 15-30ºC N/A


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Trace Elements

Trace Elements B (1,000x)

Trace Elements B solution has the same composition as Cleveland’s Trace Element I. The combination of Trace Elements B contains the trace elements of MCDB 104 and of MCDB 301


VWR Cat. No.


45000-714 25-022-CI 100 mL 1 15-30ºC 2-8ºC N/A

Trace Elements C (1,000x)

Trace Elements C solution has the same composition of Cleveland’s Trace Elements II, and the combination of Trace Elements C contains the trace elements of MCDB 301


VWR Cat. No.


45000-716 25-023-CI 100 mL 1 15-30ºC 2-8ºC N/A

Tris Base Buffer, powderTris is used in buffer solutions in biochemical and molecular biological research. This pri-mary amine has an effective pH range of 7.0-9.2 and inhibits a number of different enzymes, limiting its usefulness in protein studies to some extent. This tris base buffer is provided in powder form.


VWR Cat. No.


62111-160 61-233-RM 100 g 1 15-30ºC 15-30ºC ≥ 12 Months62111-158 61-233-RR 1 kg 1 15-30ºC 15-30ºC ≥ 12 Months62111-162 61-233-RT 5 kg 1 15-30ºC 15-30ºC ≥ 12 Months

Trypan BlueTrypan blue is the most common stain used in cell count and viability assays. Viable cells exclude the dye, while nonviable cells absorb the dye and appear blue. Cells should be in suspension as single cells in buffered saline before counting. Trypan blue can be used in assays providing total cell count, viable cell count or nonviable cell count alone. This solution is provided at the common concentration of 0.4% (w/v).

Trypan Blue Solution � 0.4% (w/v) in normal saline (8.1 g/L NaCl with 0.6 g/L K2HPO4) Final pH = 7.5 ± 0.5, � Osmolality (mOsm) = 305 ± 35 � Intended Use: In Vitro Diagnostic

VWR Cat. No.


45000-717 25-900-CI 100 mL 1 15-30ºC 15-30ºC 24 Months


61

I N S E C T M E D I A A N D R E A G E N T SInsect M

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63

Insect Media and Reagents

Grace’s Insect Basal Medium 64Hink’s TNM-FH Medium 64insectagro™ Reagents 64insectagro™ Serum-Free Media 65

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Grace’s Insect Basal Medium (Vaughn Mod.)The insect cell culture medium was formulated to closely resemble insect hemolymph, a compound crucial to the function of the insect circulatory system. The basal medium, when properly supplemented, has been used to culture cells derived from a variety of insects includ-ing several species of lepidopterans as well as some dipterans. The medium is primarily used as a basal medium for the growth and maintenance of cell lines derived from lepidopterans.

Grace’s Insect Basal Medium (Vaughn Mod.), 1x � With L-glutamine, without insect hemolymph � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-476 13-200-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

Hink’s TNM-FH Medium (Mod.) (Supplemented Grace’s Medium)Hink’s TNM-FH medium is a modified Grace’s formulation containing lactalbumin hydrolysate (LAH) and ultrafiltered yeastolate, and was originally developed for the optimal propagation of cabbage looper, Tricoplusi in cell.

Hink’s TNM-FH Medium (Mod.), 1x � With L-glutamine, lactalbumin hydrolysate, and UF Yeast Extract, without insect hemolymph


VWR Cat. No.


45000-478 13-100-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

insectagro™ ReagentsPoloxamer 188

� Includes a non-ionic detergent that protects cells from hydrodynamic damage � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-488 13-901-CI 100 mL 6 15-30ºC 2-8ºC 12 Months

Poloxamer 188, Powder � Includes a non-ionic detergent that protects cells from hydrodynamic damage � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-486 61-161-RM 100 g 1 15-30ºC 15-30ºC ≥ 12 Months

I N S E C T M E D I A A N D R E A G E N T SInsect M

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65

insectagro™ Serum-Free MediaInsect cell culture has become an increasingly important tool in biotechnology for the pro-duction of biologicals. The inherent growth characteristics of the insect cells make them ideal candidates for Baculovirus Expression Vector Systems (BEVS) for the production of recombinant genes becasue they provide advantages over both bacterial and mammalian cultures, due to their fast doubling times and ability to grow in suspension. Insect cells have significantly impacted protein production through the use of large-scale bioreactors to produce recombinant proteins and products of medical and agricultural importance with high expression levels via BEVS.

Conventional insect culture media rely heavily on animal sera and other components such as yeast hydrolysate, lactalbumin hydrolysate, tryptose phosphate broth, and hemolymph as the nutritional, chemical, and physiological support systems. However, the production of purified recombinant protein has created a demand for media capable of supporting growth without relying on these additives.

Serum-free growth media poses numerous advantages over the use of serum, including:

� Reduced foaming in most bioreactors � Elimination of exogenous agents � Easier downstream processing � Support of high cell yields

insectagro DS2™ Serum-Free/Protein-Free Medium, 1x

insectagro DS2 Serum-Free/Protein-Free Medium was developed for the growth and main-tenance of Drosophila Schneider 2 (S2) cells to be used in heterologous protein expression. At the optimal temperature range 22-24°C, DS2 cells grow as a loose monolayer and are readily adaptable to growth in suspension. Under these conditions, the cells require minimal adaptation to serum-free culture.

� Without L-glutamine � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45000-474 13-402-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

insectagro Sf9™ Serum-Free/Protein-Free Medium

insectagro Sf9™ is formulated to support the propagation of Sf9 insect cells in culture, and can also be used for SF21 cells. Sf9 cells are cultured in non-humidified, non-CO2 incuba-tors at 27ºC (room temperature) and display both monolayer and suspension culture qualities. With their fast doubling times of 18-22 hours, Sf9 cells are easily scaled up to large cultures using bioreactors.

� With L-glutamine � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-054 13-410-CV 500 mL 6 15-30ºC 2-8ºC 12 Months

M I C R O B I O L O G Y & M O L E C U L A R B I O L O G Y

67

Microbiology and

Molecular Biology

Microbiology and Molecular Biology

Miller’s LB Broth 68S.O.C Medium 68Terrific Broth 68Ampicillin 68Carbenicillin 69Kanamycin Sulfate 69Neomycin Sulfate 69Streptomycin Sulfate 69Tetracycline Hydrochloride 70X-Gal 70Isopropylthiogalactoside (IPTG) 70Phosphate Buffered Saline (PBS), 10x 71SSC, 20x, Liquid 71SSPE, 20x, Liquid 71TAE Buffer, 10x 72TBE Buffer, 10x, Liquid 72TE Buffer, 1x, Liquid 72Tris Buffered Saline, 10x, Liquid 73Tris-Hydrochloride Buffers, 1M 73EDTA, 0.5M, pH 8.0 74Molecular Biology Grade Water 74Reagent Grade Water, 4L Cube 74MOPS Buffer 75SDS (Sodium Dodecyl Sulfate), 10% 75Sodium Acetate, 3M 75Sodium Chloride, 5M 75



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Microbiological Media and Components

Miller’s LB BrothMiller’s LB broth is based on the original Lysogeny Broth (LB) media formulation devel-oped by Luria and Bertani, which was designed for the rapid propagation of recombinant E. coli strains. Miller’s LB contains a 20-fold concentration sodium chloride compared to the original formulation, and is widely used for the growth and maintenance of cells in work with plasmid DNA and recombinant protein production.

� 10.00 g/L casein peptone, 10.00 g/L NaCl, 5.00 g/L UF yeast powder � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-048 46-050-CM 1L 6 15-30ºC 15-30ºC N/A

S.O.C MediumS.O.C medium is used as a recovery medium during the final stage of transformation of E. coli. The use of SOC medium improves transformation efficiency.

� 20.0 g/L casein peptone, 5.0 g/L yeast extract, 0.5845 g/L NaCl, 0.1864 g/L KCl, 0.9521 g/L MgCl2, 1.2036 g/L MgSO4, 3.6032 g/L glucose


VWR Cat. No.


45001-099 46-003-CM 10 mL 10 15-30ºC 15-30ºC 12 Months

Terrific BrothTerrific Broth is a medium for the propagation and maintenance of recombinant E. coli. Because this medium supports higher cell densities than LB medium, it is preferred for maxi-mizing plasmid yield in preparation for nucleic acid purification.

� 12.00 g/L casein peptone, 4.00 (mL) glycerol, 2.31 g/L KH2PO4 , 12.54 g/L K2HPO4 , 24.00 g/L UF yeast powder


VWR Cat. No.


45001-050 46-055-CM 1L 6 15-30ºC 15-30ºC 12 Months

Ampicillin, Sodium Salt, PowderAmpicillin is a penicillin derivative that inhibits bacterial cell-wall synthesis by inactivating transpeptidases on the inner surface of the bacterial cell membrane. A β-lactam antibiotic with an amino group side chain attached to the penicillin structure, its mode of resistance is through the cleavage of β-lactam ring of ampicillin by β-lactamase. Ampicillin is effective against Gram-positive and some Gram-negative bacteria.


VWR Cat. No.


45000-612 61-238-RH 10 g 1 15-30ºC 2-8ºC 24-30 Months

45000-614 61-238-RM 100 g 1 15-30ºC 2-8ºC 24-30 Months


69

Microbiology and

Molecular Biology

Carbenicillin, Disodium SaltCarbenicillin is a form of penicillin and is often used in place of ampicillin because it main-tains stability after being autoclaved. Like ampicillin, carbencillin interferes with cell-wall synthesis of actively growing cells.

� Molecular Weight = 422.4 � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-094 46-100-RG 5 g 1 15-30ºC 2-8ºC 24-30 Months

Kanamycin SulfateKanamycin is a member of the aminoglycosides a group of molecules and interacts with the 30S subunit of prokaryotic ribosomes. It induces errors in translation and thus hinders protein synthesis. The KanR-Tn5 gene product (aminoglycoside phosphotransferase) confers resistance to kanamycin. Kanamycin Sulfate is effective against Gram-negative and Gram–positive bacteria.

Kanamycin Sulfate, Liquid

VWR Cat. No.


45000-810 30-006-CF 50 mL 6 -15-20ºC -15-20ºC 12 Months

Kanamycin Sulfate, Powder

VWR Cat. No.


45000-640 61-176-RG 5 g 1 15-30ºC 15-30ºC ≥ 12 Months

Neomycin SulfateNeomycin, a nucleoside antibiotic and aminoglycoside, is a protein synthesis inhibitor that causes premature chain termination by acting as an analog of the 3’-terminal end of amino-acyl-tRNA. It prevents growth of bacteria, protozoa, algae, and mammalian cells. Organisms carrying the resistance gene puromycin acetyltransferase are effectively protected from this compound. Neomycin is effective against Gram-negative and Gram-positive bacteria.


VWR Cat. No.


45000-644 61-241-RG 5 g 1 15-30ºC 15-30ºC 12 Months

Streptomycin SulfateStreptomycin is an antibiotic which acts as a protein synthesis inhibitor. This aminoglycoside acts by binding to the 30S ribosomal subunit, preventing protein production and leading to bacterial cell death. Streptomyicin is more effective against Gram-positive bacteria than Gram-negative. It is offered in a powder form.


VWR Cat. No.

Corning Cat. No.


45000-654 61-088-RM 100 g 1 15-30ºC 2-8ºC 12 Months



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Tetracycline Hydrochloride, PowderTetracycline Hydrochloride (HCl) is an antibiotic that inhibits protein synthesis by binding to the 30S ribosomal subunit, blocking interactions at the acceptor site on the RBA-ribosome complex. It is a member of the tetracycline group of antibiotics, a broad-spectrum class that inhibits growth of both aerobic and anaerobic organisms through this common mechanism. The congener tetracycline is semi-synthetically produced from chlortetracycline, a product of Staphylococcus aureofaciens. Resistance is conferred by the TetR-Tn10 gene product that blocks cell wall permeability. Tetracycline HCl is effective against Gram-positive and Gram-negative bacteria and is offered in powder form.


VWR Cat. No.


45000-656 61-242-RG 5 g 1 2-8ºC 2-8ºC 12 Months

X-Gal, PowderX-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside) is a substrate of b-galactosidase, a gene product of the lac operon in E. Coli. X-Gal included in bacterial medium will be hydrolyzed by ß-galactosidase to produce indoxyl, a colorimetric indicator of the activity of the lac operon.


VWR Cat. No.


45001-092 46-101-RF 1 g 1 -15-25ºC -15-25ºC 24 Months

Isopropylthiogalactoside (IPTG), PowderIPTG (isopropylthiogalactoside) is an inducer of the lac operon in bacteria, which is frequently used in cloning as a component of a recombinant plasmid. When used with X-Gal to detect lac gene activity, IPTG can be used for screening of clones containing a gene of interest in the lac operon.


VWR Cat. No.


45001-084 46-102-RF 1 g 1 -15-25ºC -15-25ºC 36 Months


71

Microbiology and

Molecular Biology

Buffers

Phosphate Buffered Saline (PBS), 10x, pH 7.4Phosphate buffered saline is used to maintain a physiological pH and osmolarity, two key factors in tissue culture and molecular biology research. This concentrated form should be diluted to a working concentration using reagent grade water.

� Without calcium and magnesium pH: 7.4 ± 0.1, DNase, RNase, protease tested � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-130 46-013-CM 1L 6 15-30ºC 15-30ºC 24 Months

SSC, Liquid, 20x20x Saline Sodium Citrate (SSC), 20x, buffer is used in both nucleic acid hybridization and blot transfer procedures. 20x is the maximum concentration used; 0.2x may be used depend-ing on the application.

� pH 7.0 ± 0.1, DNase, RNase, protease tested � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-046 46-020-CM 1L 6 15-30ºC 15-30ºC 24 Months

SSPE, 20x, LiquidSaline Sodium Phosphate EDTA (SSPE), 20x, buffer has applications in nucleic acid hybridization and other molecular biology work.

� pH 7.4 ± 0.1 (20-25ºC) @ 20x, DNase, RNase, Protease tested � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-128 46-021-CM 1L 6 15-30ºC 15-30ºC 18 Months



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TAE Buffer, 10xTris Acetate EDTA (TAE) buffer is used in DNA agarose gel electrophoresis, both in the aga-rose gel itself and the running buffer. Linear, double-stranded DNA separates faster in a TAE buffer-based system compared to Tris Borate EDTA (TBE) buffer, but the latter has a higher buffering capacity and is thus more resistant to changes in pH. Use of TAE vs TBE buffer depends on downstream applications. TAE is recommended for preparative electrophoresis in which the nucleic acid bands being separated are to be used in cloning, and other work requiring enzymatic applications.

� pH 8.3 ± 0.1 (24-26ºC), DNase, RNase, protease tested � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-074 46-010-CM 1L 6 15-30ºC 15-30ºC 24 Months

TBE Buffer, 10x, LiquidTBE (Tris-Borate-EDTA) has applications in DNA and RNA agarose gel electrophoresis and polyacrylamide gel electrophoresis. TBE has a higher buffering capacity than Tris-Acetate-EDTA (TAE) buffer, but the latter is more suitable for steps involving the action of enzymes on nucleic acids because borate is an enzymatic inhibitor. Electrophoresis using TBE-con-taining agarose gels and TBE running buffer is much more suitable for size analysis of the nucleic acids being separated, as TBE buffer allows these nucleic acids to better maintain their structural integrity.

� pH: 8.4 ± 0.1 (24-26ºC), DNase, RNase, protease tested � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-072 46-011-CM 1L 6 15-30ºC 15-30ºC 24 Months

TE Buffer, 1x, LiquidTris-EDTA (TE) buffer is commonly used in work with nucleic acids. It can be included as a solubilization buffer in DNA and RNA purification, as a running buffer in electrophoresis, and as a general storage buffer for nucleic acids.

� pH: 7.9-8.1 (20-25ºC), DNase, RNase, protease tested � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-154 46-009-CM 1L 6 15-30ºC 15-30ºC 12 Months


73

Microbiology and

Molecular Biology

Tris Buffered Saline, 10x, LiquidTris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical tech-niques to maintain pH within a relatively narrow range. TBS can shift pH relatively dramati-cally as temperature changes, with the pKa declining approximately 0.03 units per degree Celsius rise in temperature.

� pH: 7.4 ± 0.1 (24-26ºC) @ 1x, DNase, RNase, protease tested � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-132 46-012-CM 1L 6 15-30ºC 15-30ºC 12 Months

Tris-Hydrochloride Buffers, 1mTris-HCI buffer has many applications in molecular biology work, including use in nucleic acid purification. This product can be diluted to obtain the desired concentration.

1M Tris-HCl, pH 7.5, Liquid � pH: 7.5 ± 0.1 (24-26ºC), DNase, RNase, protease tested � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-066 46-030-CM 1L 6 15-30ºC 15-30ºC 24 Months

1M Tris-HCl, pH 8.0, Liquid � pH: 8.0 ± 1.0 (24-26ºC), DNase, RNase, protease tested � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-064 46-031-CM 1L 6 15-30ºC 15-30ºC 24 Months



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Reagents

EDTA, 0.5m, pH 8.0EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to “sequester” metal ions such as Ca2+ and Fe3+. These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution, but exhibit diminished reactivity. The use of EDTA allows for greater control over many experiments.

� 0.5 EDTA, pH 8.0 � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-122 46-034-CI 100 mL 6 15-30ºC 15-30ºC 24 Months

Molecular Biology Grade WaterMolecular biology grade water is ideal for the preparation of reagents, rinsing glassware and plasticware, and other molecular biology applications where Rnase, Dnase, and Protease-free water is required. No toxic agents, including DEPC, are used in the preparation of our molecular biology grade water, eliminating possible interferences with enzymatic reactions.

� pH 6.0 ± 1.0, DNase, RNase, protease tested � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


95000-094 46-000-CI 100 mL 6 15-30ºC 15-30ºC 24 Months

91002-726 46-000-CV 500 mL 6 15-30ºC 15-30ºC 24 Months

45001-044 46-000-CM 1L 6 15-30ºC 15-30ºC 24 Months

Reagent Grade Water, 4L CubeReagent grade water is ideal for the preparation of reagents, rinsing glassware and plastic ware, cleaning electrodes and other common laboratory tasks requiring highly pure water. No toxic agents, including DEPC, are used in its preparation. The packaging design allows for portability, ease of storage and convenient dispensing. Upon packaging, the water meets USP conductivity requirements for water for injection (WFI) and is RNAse, DNAse and protease free.

VWR Cat. No.


97060-878 46-002-LF 4L 2 15-30ºC 15-30ºC N/A


75

Microbiology and

Molecular Biology

MOPS BufferMOPS is a zwitterionic buffer used in denaturing agarose gel electrophoresis of RNA and in SDS-PAGE protein analysis. It has a pH range of 6.5 - 7.9.

� Molecular Weight = 209.27 � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-134 46-103-RM 100 g 1 15-30ºC 15-30ºC N/A

SDS (Sodium Dodecyl Sulfate), 10%Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2 µm filtered.


VWR Cat. No.


45001-120 46-040-CI 100 mL 1 15-30ºC 15-30ºC 24 Months

Sodium Acetate, 3mSodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution.

� pH 5.2 ± 0.1 (20-25ºC), � DNase, RNase, protease tested � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-124 46-033-CI 100 mL 1 15-30ºC 15-30ºC 24 Months

Sodium Chloride, 5m5M Sodium Chloride, (NaCl) is a common molecular biology reagent. It is tested to ensure that they are free of RNAse. This product is used to control osmolality and conductivity, among other factors.

� DNase, RNase, protease tested � Intended Use: Research Use Only, Not for IVD Use

VWR Cat. No.


45001-125 46-032-CV 500 mL 6 15-30ºC 15-30ºC 24 Months

H I G H Q U A L I T Y W A T E R

77

High Q

uality Water

High Quality Water

Distilled, Deionized Water 79USP/EP Certified Sterile WFI Quality Water 79



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High Q

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Sterile WFI-Quality, Cell Culture Grade WaterCorning cellgro’s state-of-the-art in-house water system provides purified water, free of any added components, ideal for many applications in the research and biopharmaceutical industry. This advanced process incorporates innovative techniques to reduce bioburden and maintain low endotoxin levels. Applications include: Raw material for manufacturing, cell culture, process validation, device processing, purification buffers, and chromatography buffers.

Distilled, Deionized Water � Meets WFI quality standards � Intended Use: In Vitro Diagnostic

VWR Cat. No.


95000-092 25-055-CI 100 mL 6 15-30ºC 15-30ºC 36 Months

45000-672 25-055-CV 500 mL 6 15-30ºC 15-30ºC 36 Months

45001-042 25-055-CM 1L 6 15-30ºC 15-30ºC 36 Months

45000-674 25-055-LB 20L 1 15-30ºC 15-30ºC 36 Months

45001-270 25-055-LG 100L 1 15-30ºC 15-30ºC 36 Months

45001-088 25-055-LH 200L 1 15-30ºC 15-30ºC 36 Months

USP/EP Certified Sterile WFI-Quality WaterUSP/EP Certified Water is validated to the most recent specifications found in the United States Pharmacopeia (USP) and the European Pharmacopeia (EP). The specific testing is in accordance to testing for “Sterilized Water for Injection”, (European Pharmacopeia), and “Sterile Water for Injection”, (United States Pharmacopeia).

This product is intended for Further Manufacturing Use Only in a multitude of applications, from used with Steam-in-Place (SIP) systems and equipment to Clean-in-Place (CIP) systems and synthesis of a final product. This water is also suitable for product reconstitution and for use as a cleaning agent for rinsing vessels and equipment.

USP/EP Certified Sterile WFI-Quality Water � USP/EP Certified � Intended Use: In Vitro Diagnostic

VWR Cat. No.


95029-816 25-065-LB 20L 1 15-30ºC 15-30ºC 36 Months

95029-818 25-065-LG 100L 1 15-30ºC 15-30ºC 36 Months

95029-820 25-065-LHÍ 200L 1 15-30ºC 15-30ºC 36 Months

Alphabetic IndexAAmino Acids and Vitamins 51Amphotericin B 52Ampicillin 52Ampicillin, Sodium Salt 68Antibiotic-Antimycotic Solution 53Antibiotics and Antimycotics 52

BBlasticidin S HCl 53Buffered Salt Solutions 24Buffers 56, 71

CCarbenicillin, Disodium Salt 69Cell Culture Reagents 50cellgro COMPLETE™ 32Cellstripper™ 58Charcoal-Stripped FBS 46Chloramphenicol 53Ciprofloxacin Hydrochloride 53Classical Media 6CMRL 1066 7

DDialyzed FBS 46Dimethyl Sulfoxide 60DMEM 7DMEM/Ham’s F-12 50/50 Mix 11Donor Calf Serum 44Donor Horse Serum 45Dulbecco’s Phosphate Buffered Saline 25

EEDTA, 0.5M, pH 8.0 74ES Cell Tested FBS 46

FF-12K Nutrient Mixture 12Fetal Bovine Serum 43

GG418 Sulfate 53Gamma-Irradiated FBS 47Gentamicin Sulfate 54Glasgow’s MEM 12Glucose Solution 60glutagro™ 51Grace’s Insect Basal Medium 64Growth Supplements 58

HHam’s F-10 Medium 13Ham’s F-12 Medium 13Hanks’ Balanced Salt Solution 26HAT 58HEPES 56Hink’s TNM-FH Medium 64Human AB Serum 45Hybridoma Reagents 58Hygromycin B Solution 54

IImproved MEM (Richter’s Mod.) 14insectagro DS2™ 33, 65insectagro Sf9™ 33, 66insectagro™ Reagents 64insectagro™ Serum-Free Media 65Insect Media and Reagents 63Insulin-Transferrin-Selenium 58Iscove’s Modification of DMEM 14Isopropylthiogalactoside 70

KKanamycin Sulfate 54, 71

LLeibovitz’s L-15 15L-Glutamine 51Low IgG FBS 47Lymphocyte Separation Medium 34

MMcCoy’s 5A 15MCDB 131 15Medium 199 16MEM Amino Acids 52MEM Vitamins 52Microbiology and Molecular Biology 69Miller’s LB Broth 70Minimum Essential Medium 17Miscellaneous Reagents 59Molecular Biology Grade Water 76MOPS Buffer 75

NNeomycin Sulfate 55, 71Non-Enzymatic Cell Dissociation Agents 60

PPenicillin Streptomycin Solution 55Phosphate Buffered Saline 26Phosphate Buffered Saline, pH 7.4 71Polysucrose 400 34Porcine Serum 45Puromycin Dihydrochloride 55

RReagent Grade Water 74rhAlbumin 35rhLactoferrin 36RPMI 1640 20

SSera 40Serum-Free and Speciality Media 32S.O.C Medium 68Sodium Acetate 3M 75Sodium Bicarbonate 56Sodium Chloride 5M 75Sodium Dodecyl Sulfate 10% 75Sodium Pyruvate 60Specialized Serum 46SSC, 20x 71SSPE, 20x 71stemgro® AB2™ Neural Base Media 36stemgro® ABS™ Neural Supplement 36Streptomycin Sulfate 55, 69

TTAE, 10x 72TBE Buffer, 10x 72TE Buffer, 1x 72Terrific Broth 68Tetracycline Hydrochloride 55, 70Tetracycline Negative FBS 47Trace Elements 60transfectagro™ 21Tris Base Buffer 61Tris Buffered Saline, 10x 73Tris-Hydrochloride Buffers, 1m 73Trypan Blue 61Trypsin 57Tryptic Soy Broth 35

WWater 79

XX-Gal 70

Numeric IndexCorning cellgro Category Number10-010 1810-013 810-014 710-016 1410-017 810-022 1710-024 1410-025 1210-026 1410-040 2010-041 2110-043 22 10-045 1510-050 1610-051 1610-060 1610-070 1310-080 1510-090 1310-092 1310-101 910-102 910-104 2013-100 6413-200 6413-402 33, 6513-410 33, 6613-415 33, 6613-901 6415-010 1815-012 1715-013 815-015 1815-016 1415-017 815-018 915-040 2015-041 2115-090 1115-100 1515-110 716-405 1117-104 2117-105 2017-204 917-205 917-206 917-305 1820-021 2620-023 26, 3220-030 2520-031 2521-020 2721-021 2721-022 2721-023 2721-030 2521-031 25

21-040 2625-000 6025-005 5125-015 51, 5225-021 5925-022 6025-023 6025-025 52, 5325-030 5225-035 5625-037 6025-040 3625-046 5825-047 5825-050 5725-051 5725-052 5725-053 5725-054 5725-055 5925-056 5825-060 5625-065 5925-072 3425-800 5825-900 6125-950 6030-001 5530-002 5530-003 5230-004 5330-005 5430-006 54, 6930-009 5530-234 5330-240 5435-010 4435-011 4435-015 4335-016 4335-022 4435-030 4535-040 4535-060 4535-070 43, 46, 4735-071 43, 4635-072 43, 4635-073 46, 4735-074 4435-075 44, 4740-101 3240-102 3240-200 32, 3440-300 2140-400 3646-000 7446-002 7446-003 68, 75

46-009 7446-010 7246-011 7246-012 7346-013 26, 7146-020 7146-021 7146-030 7346-031 7346-032 7546-034 7446-040 7546-050 6846-055 6846-060 3546-100 6946-101 7046-102 7046-103 7550-003 1050-004 1250-010 1950-011 1950-012 1750-013 1050-020 2150-040 1350-045 1550-050 1650-051 1751-010 1955-021 2755-022 2755-031 2561-030 5161-034 5661-065 5661-088 55, 6961-098 5461-161 6461-176 54, 6961-233 6161-234 5461-238 52, 6861-239 5361-241 55, 6961-242 55, 7061-277 5361-385 5561-411 3562-450 3562-451 3690-009 1990-013 1090-050 1790-090 1290-091 1290-113 10

Numeric IndexVWR Category Number45000-300 1745000-302 1745000-304 845000-306 845000-308 845000-310 845000-312 845000-314 845000-316 845000-318 845000-320 845000-322 845000-324 845000-326 845000-328 945000-330 945000-332 845000-334 845000-336 945000-340 945000-342 945000-344 1145000-346 1145000-348 1145000-350 1145000-352 1145000-354 1245000-356 1345000-358 1345000-360 1345000-362 1445000-364 1445000-366 1445000-368 1445000-370 1445000-372 1545000-374 1645000-376 1645000-378 1645000-380 1845000-382 1845000-384 1845000-386 1845000-388 1845000-394 1845000-396 2045000-398 2045000-400 2045000-402 2045000-404 2045000-406 2045000-408 2045000-410 2045000-412 2145000-414 2145000-416 2145000-418 2145000-420 3245000-422 3245000-424 3245000-426 25

45000-428 2545000-430 2545000-432 2545000-434 2545000-436 2545000-438 2545000-440 2545000-442 2545000-444 2545000-446 2645000-448 2645000-450 2645000-452 2745000-454 2745000-456 2745000-458 2745000-460 2745000-462 2745000-464 2745000-466 2745000-470 2745000-474 33, 6545000-476 6445000-478 6445000-486 6445000-488 6445000-504 1745000-518 1045000-520 1045000-522 1045000-524 1045000-526 1045000-534 1045000-536 1245000-540 1245000-544 1245000-550 1345000-562 1545000-570 1645000-574 1745000-578 1745000-582 1945000-584 1945000-586 1945000-592 1945000-594 1945000-596 1945000-602 2145000-604 2145000-612 52, 6845000-614 5245000-616 5345000-618 5345000-620 5345000-622 5345000-624 5445000-626 5445000-628 5445000-630 5345000-632 5445000-634 54

45000-636 5445000-640 54, 6945000-644 55, 6945000-650 5545000-652 5545000-654 55,6945000-656 55, 7045000-658 5745000-664 5745000-666 5745000-668 5845000-672 7945000-674 7945000-676 5145000-678 5145000-680 5145000-682 5145000-684 5145000-686 5845000-688 5845000-690 5645000-692 5645000-694 5645000-696 5645000-698 5245000-700 5245000-702 5245000-704 5645000-706 5645000-708 5645000-710 5945000-712 5945000-714 6045000-716 6045000-717 6045000-726 3445000-728 3445000-730 3445000-732 3445000-734 4445000-736 4445000-806 5445000-808 5245000-810 54, 6945001-042 7945001-044 7445001-046 7145001-048 6845001-050 6845001-052 1145001-054 33, 6545001-058 4545001-060 4445001-062 4545001-064 7345001-066 7345001-072 7245001-074 7245001-076 5345001-082 5745001-084 70

45001-086 5145001-088 7945001-090 5845001-092 7045001-094 6945001-099 6845001-101 2745001-103 5545001-106 4345001-108 4345001-110 2145001-112 1545001-114 745001-116 5945001-118 5945001-120 7545001-122 7445001-124 7545001-125 7545001-128 7145001-130 26, 7145001-132 7345001-134 7545001-152 2645001-154 7245001-156 3545001-226 3545001-270 7945001-660 5745001-662 5762111-158 6062111-160 6062111-162 6062111-170 5571002-674 1171002-676 5371002-816 3371002-872 971002-874 971002-876 1271002-878 2089133-078 3589133-080 3691002-726 7495000-092 7995000-094 7495029-816 7995029-818 7995029-820 7997060-260 3697060-262 3697060-876 997060-878 7497065-300 43, 46, 4797065-302 43, 4697065-304 43, 4697065-306 44, 4797065-308 4497065-310 44, 47

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