Cell Biology I. Cellular structure and...
Transcript of Cell Biology I. Cellular structure and...
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1. Review basic concepts and new development
2. Current methodologies and techniques
3. Reading scientific literature
4. Research processCollect background informationIdentify scientific questionsFind experimental approachesProcess and interpret dataDraw conclusions
5. Writing research proposals
Cell Biology I. Cellular structure and function
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Cell Biology I. Topics
Cell membrane and organelles
Introduction of cell signaling
Membrane trafficking, secretory and endocytic pathways
Cytoskeleton
Cell cycle
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Cell membrane
Wenxia:
1. The concept of cells
2. Prokaryotic cells, eukaryotic cells, mammalian cells
3. Cell membrane: plasma membrane and internal membrane
Wenxia:
1. The concept of cells
2. Prokaryotic cells, eukaryotic cells, mammalian cells
3. Cell membrane: plasma membrane and internal membrane
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The bilayer structure of biomembranes
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The faces of cellular membraneWenxia:
1. Exoplasmic and cytosolic face and its importance
2. Compartment with single and double bilayers
3. Different protein and lipid composition of different membranes
Wenxia:
1. Exoplasmic and cytosolic face and its importance
2. Compartment with single and double bilayers
3. Different protein and lipid composition of different membranes
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Three major classes of membrane lipidsWenxia:
The relationship of the head group, fatty acid and lipid composition with the thickness, curve and fluidity of membrane
Wenxia:
The relationship of the head group, fatty acid and lipid composition with the thickness, curve and fluidity of membrane
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Gel and fluid forms of the lipid bilayer
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The fluid mosaic model of cell membrane
Initially proposed by Singer and Nicolson 1972
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Proteins associated with the lipid bilayer
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Glycophorin A E. coli Porin
Transmembrane proteins
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adhesion moleculesglycosylphosphtidylinositol
Proteins with covalently linked lipid anchors
(a) Prenylation (b) Acylation
Ras Src
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Proteins binds to lipids
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Lipid rafts
By Susan Pierce, Nature Reviews Immunology 2, 96-105 (2002)
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Lipid rafts
Atomic force microscopy
Fluorescence microscopy
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Organelles of the eukaryotic cell
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Fluorescence-activated cell sorter (FACS)
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Dot plot of FACS
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Purification of cell population by magnetic beads
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Differential centrifugation is a common first step in fractionating a cell homogenate
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Separation of subcellular organelle by equilibrium density gradient centrifugation
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Fluorescence microscopy
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Localization of the B cell antigen receptor in lipid rafts
Cholera toxin B subunitsAnti-BCR antibody
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Deconvolution fluorescence microscopy
DNA/microtubules/actin filaments
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Transmission electron microscopy
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Scanning electron microscopy (SEM)
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Journal discussion
Singer S. J. and Nicolson G. L. The fluid mosaic model of the structure of cell membrane. 1972. Science 175: 720.
Harder T., Scheiffele P., Verkade P. and Simons K. Lipid domain structure of the plasma membrane revealed by patching of membrane components. 1998. Journal of Cell Biology 141:929.