CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States
description
Transcript of CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States
![Page 1: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/1.jpg)
CDC perspective on non-O157
Shiga toxin-producing E. coli (STEC) in the United States
Patricia M. Griffin, M.D.Chief, Enteric Diseases Epidemiology BranchOctober 17, 2007
![Page 2: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/2.jpg)
E. coli that cause human gastrointestinal illness Shiga toxin-producing (STEC), also called
Enterohemorrhagic (EHEC) Enteropathogenic (EPEC) Enterotoxigenic (ETEC) Enteroinvasive (EIEC) Other types, less well characterized
![Page 3: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/3.jpg)
E. coli that cause human gastrointestinal illness Shiga toxin-producing (STEC), also called
Enterohemorrhagic (EHEC) E. coli O157 serogroup Non-O157 serogroups
Enteropathogenic (EPEC) Enterotoxigenic (ETEC) Enteroinvasive (EIEC) Other types, less well characterized
![Page 4: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/4.jpg)
Animals are the reservoirs for STEC Cattle Other ruminants Other animals
especially those who have contact with cattle
![Page 5: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/5.jpg)
Major modes of transmission of STEC to humans – how the fecal matter gets to the mouth Food
cattle products, e.g., beef, raw milk food contaminated with cattle or human feces e.g.,
lettuce Water
Drinking water Recreational water
Animal contact contact with farm animals, e.g. petting zoos contact with farm animals’ environment
Person contact With the feces of infected persons
![Page 6: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/6.jpg)
Sequence of events in E. coli O157:H7 infection
E. coli O157 ingested
3 - 4 days
non-bloody diarrhea, abdominal cramps
5 - 6 daysresolution
92% 8%
HUS
bloody diarrhea1 - 2 days80%
Mead. Lancet 1998
![Page 7: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/7.jpg)
Non-O157 STEC ingested
3 - 4 days
5 - 6 daysresolution
98%?bloody diarrhea
1 - 2 days40%
Sequence of events in non-O157 STEC infection
non-bloody diarrhea, abdominal cramps
rare
HUS
![Page 8: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/8.jpg)
Compared to persons with E. coli O157 infection,
persons with non-O157 STEC have less severe illness
But non-O157 STEC include many serogroups, with varying virulence some typically cause only mild diarrhea others can cause HUS and death
![Page 9: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/9.jpg)
Clinical lab testing for STEC E. coli O157
Unusual feature: does not ferment sorbitol streak stool specimen onto plate containing
Sorbitol-MacConkey (SMAC) medium• select clear colonies (others are pink)
– O157 strains agglutinate when O157 antisera is added
Non-O157 STECLack unusual features, look like good
E. coli
![Page 10: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/10.jpg)
Timeline of public health recommendations for STEC
1994 E. coli O157 infection made reportable
1995 Commercial Shiga toxin enzyme immunoassay (EIA) introduced
2000 Non-O157 STEC infections made nationally reportable
![Page 11: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/11.jpg)
Testing for non-O157 STEC using the Shiga toxin EIA
Clinical lab cultures stool specimen in broth tests broth for Shiga toxin using EIA
positive test could be O157 or non-O157 STEC Clinical lab can send Shiga toxin-positive broth
to State Health lab State Health lab isolates STEC
State Health Lab sends STEC to CDC• CDC determines serotype
![Page 12: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/12.jpg)
After adopting the EIA, some clinical labs stopped testing for E. coli O157 using selective media E. coli O157 outbreaks could be missed
Some clinical labs discard Shiga toxin-positive specimens without obtaining an isolate, so simply report “Shiga toxin positive” to doctor serogroup not determined
E. coli O157 strains not identified and sub-typed for outbreak detection
Non-O157 outbreaks less likely identified
Some challenges arising from use of the Shiga toxin EIA
![Page 13: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/13.jpg)
How do we learn about non-O157 STEC?
FoodNet conducts active surveillance Some clinical labs isolate non-O157 STEC
strains are serotyped at CDC Some health departments are doing studies, e.g.,
Minnesota Connecticut
Outbreak investigations Studies of HUS
![Page 14: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/14.jpg)
How do we learn about non-O157 STEC?
FoodNet conducts active surveillance Some clinical labs isolate non-O157 STEC
strains are serotyped at CDC Some health departments are doing studies, e.g.,
Minnesota Connecticut
Outbreak investigations Studies of HUS
![Page 15: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/15.jpg)
FoodNet Catchment Area, 2007
Catchment population 45 million persons(15% of U.S. population)
California
Oregon
New Mexico
Colorado
MinnesotaNew York
Connecticut
Tennessee
Georgia
Maryland
![Page 16: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/16.jpg)
Pyramid of Surveillance
Exposed to STEC
Person becomes ill
Person seeks care
Specimen obtained
Clinical lab tests for STEC
STEC isolated
Reported to health department & CDC
![Page 17: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/17.jpg)
Pyramid of Surveillance
Exposed to STEC
Person becomes ill
Person seeks care
Specimen obtained
Clinical lab tests for STEC?
STEC isolated
Reported to health department & CDC
![Page 18: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/18.jpg)
Pyramid of Surveillance
Exposed to STEC
Person becomes ill
Person seeks care
Specimen obtained
Lab tests for pathogen
Pathogen isolated
Reported to health department
Clinical lab survey
Active surveillance
![Page 19: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/19.jpg)
Percent of clinical labs screening all stools for E. coli O157
0
20
40
60
80
100
1985
1987
1989
1991
1993
1995
1997
1999
2001
2003
2005
2007
Year
FoodNet sitesNational sample
Boyce, J Clin Micro 1995; Voetsch CID 2004; and unpublished preliminary data
Western states outbreak
% of labs
66%
![Page 20: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/20.jpg)
Percent of clinical labs that ever conduct on-site testing for STEC using EIA, FoodNet
0
5
10
15
20
2003 2007
Year
% of labs
3%
9%
Preliminary data
![Page 21: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/21.jpg)
Human isolates of non-O157 STEC, by serogroup, FoodNet sites, 2000-2006
0
5
10
15
20
25
26 111 103 45 145 121 other
O Group
% o
f Iso
late
s
42 serogroups<1.5% each
N=575 isolates*
*preliminary data; an additional 54 isolates had missing O group information
83%
![Page 22: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/22.jpg)
Number of non-O157 STEC identified in FoodNet sites, 2000-2006
0
50
100
150
200
250
2000 2001 2002 2003 2004 2005 2006Year
Num
ber o
f Iso
late
s
non-O157 STEC O antigen undetermined STEC
![Page 23: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/23.jpg)
How do we learn about non-O157 STEC?
FoodNet conducts active surveillance Some clinical labs isolate non-O157 STEC
strains are serotyped at CDC Some health departments are doing studies, e.g.,
Minnesota Connecticut
Outbreak investigations Studies of HUS
![Page 24: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/24.jpg)
Human isolates of non-O157 STEC serotyped by CDC, by serogroup, 1983-2002
0
5
10
15
20
25
26 111 103 121 45 145 other und
O Group
Brooks, JID 2005;192:1422
N = 940 isolates% of isolates55 O groups, each <1%
![Page 25: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/25.jpg)
Human isolates of non-O157 STEC serotyped by CDC, by serogroup, 1983-2002
0
5
10
15
20
25
26 111 103 121 45 145 other und
O Group
Brooks, JID 2005;192:1422
N = 940 isolates% of isolates55 O groups, each <1%
70%
![Page 26: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/26.jpg)
MA=43RI=3CT=39NJ=1MD=13
87
78
55
2
87
26
1
2
1410
9 1
24
16
13
19
12
1
1
2885
19
3
26
83
7
7
471
Human non-O157 STEC isolates submitted to CDC by states, 1983-2001
(N = 653 isolates)
1
![Page 27: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/27.jpg)
Seasonality of human non-O157 STEC isolates submitted to CDC, 1983-2002
0
20
40
60
80
100
120
140
160
180
Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec
Month
Num
ber o
f iso
late
s
(N=940 isolates)
Brooks, JID 2005
![Page 28: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/28.jpg)
Persons with HUS rarely had non-O157 STEC strains that produced only Shiga toxin 1
STEC toxin profile HUS (n= 21)
No HUS(n=271)
Only Shiga toxin 1 5% 68%
Shiga toxin 2 (+/- Shiga toxin 1)
95% 32%
Total 100% 100%
Overall, 61% of human non-O157 STEC produced only Shiga toxin 1Brooks, JID 2005
Isolates with clinical information submitted to CDC, 1983-2002
![Page 29: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/29.jpg)
How do we learn about non-O157 STEC?
FoodNet conducts active surveillance Some clinical labs isolate non-O157 STEC
strains are serotyped at CDC Some health departments are doing studies, e.g.,
Minnesota Connecticut
Outbreak investigations Studies of HUS
![Page 30: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/30.jpg)
Surveillance for STEC in all diarrheal stools
Lab A: urban
Lab B: serves a semi-rural area with agriculture and dairy farms
Minnesota
Lab B
Lab AMedus, Besser, Hedberg, Bartkus, Juni, Smith, EID Conference 2003
![Page 31: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/31.jpg)
Proportion of STEC that were O157 or non-O157, human diarrheal stools, Minnesota, 2000-2002
0.010.020.030.040.050.060.070.080.090.0
100.0
O157 Non-O157 O157 Non-O157
Urban Semi-Rural
Juni, Besser, Hunt, Smith, Hedberg, Medus,Sullivan, Bartkus, unpublished
% of STEC
![Page 32: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/32.jpg)
How do we learn about non-O157 STEC?
FoodNet conducts active surveillance Some clinical labs isolate non-O157 STEC
strains are serotyped at CDC Some health departments are doing studies, e.g.,
Minnesota Connecticut
Outbreak investigations Studies of HUS
![Page 33: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/33.jpg)
Outbreaks of non-O157 STEC infections, U.S., 1990-2007
01234
1990 1992 1994 1996 1998 2000 2002 2004 2006
N = 23 outbreaks
Data from 2007 are preliminary
No. outbreaksShiga toxin EIA available
Non-O157 STEC reportable
![Page 34: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/34.jpg)
Serogroup No. outbreaks
O111 (one outbreak also had O157) 10
O121 3
O26 3
O45 2
O27, O103, O104, O153 1 each
O26 and O121 together 1
Data from 2007 is preliminary
Serogroups of non-O157 STEC outbreaks, U.S., 1990-2007
N = 23 outbreaks
![Page 35: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/35.jpg)
Serogroup No. outbreaks
O111 (one outbreak also had O157) 10
O121 3
O26 3
O45 2
O27, O103, O104, O153 1 each
O26 and O121 1
Data from 2007 is preliminary
Serogroups of 23 non-O157 STEC outbreaks, U.S., 1990-2007
Green shows most common serogroups of sporadic cases
![Page 36: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/36.jpg)
Mode No. outbreaks
Food 11
Person-to-person 6
Lake water 3
Animal contact 2
Undetermined 1
Modes of transmission in non-O157 STEC outbreaks, U.S.,1990-2007
(N = 23)
![Page 37: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/37.jpg)
Food vehicles in non-O157 STEC outbreaks, U.S., 1990-2007
Food Vehicle No. outbreaks
Salad bar 1
Salad and ice 1
Berries 1
Milk 1
Cider 1
Punch 1
Unknown 5
N = 11
![Page 38: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/38.jpg)
CT=1
32 2
11
2
1
1
1
11
1
Human non-O157 STEC outbreaks reported to CDC, 1990-2007
(N = 23 outbreaks)
Data from 2007 is preliminary
1
1
1
2
![Page 39: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/39.jpg)
Outbreak of STEC O111 infections, cheerleading camp, Texas, 1999
55 persons with diarrhea most were teenage
girls 18 had bloody stools 2 develped hemolytic
uremic syndrome (HUS)
Transmitted by salad bar and ice
![Page 40: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/40.jpg)
How do we learn about non-O157 STEC?
FoodNet conducts active surveillance Some clinical labs isolate non-O157 STEC
strains are serotyped at CDC Some health departments are doing studies, e.g.,
Minnesota Connecticut
Outbreak investigations Studies of HUS
![Page 41: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/41.jpg)
National prospective diarrhea-associated (D+) HUS study, 1987-1991 Enrolled adults and children with D+HUS Requested
stool sample serum to measure antibodies to O157
lipopolysaccharide (LPS)
Banatvala, JID 2001
![Page 42: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/42.jpg)
Patients with both stool culture and serology results (N=55)
18% had no evidence of STEC infection 82% had evidence of STEC infection
98% of these had evidence of E. coli O157 infection
3 of 4 with non-O157 STEC isolated from stool also had antibodies to O157 LPS
• suggests that E. coli O157 may have caused their HUS
U.S. National HUS Study, 1987-1991
Banatvala, JID 2001
![Page 43: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/43.jpg)
The results of the national study suggestthat the proportion of HUS cases in the United States caused by non-O157 STEC was small
![Page 44: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/44.jpg)
Other studies of HUS with stool cultures Among HUS cases tested within 6
days of onset of diarrhea, proportion with E. coli O157:H7 isolated United States (25 cases) 96%
(Tarr, J Infect Dis 1990)Canada (30 cases) 87%
(Rowe, Epidemiol Infect 1993)
![Page 45: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/45.jpg)
Other studies of HUS with serology
Proportion of D+HUS cases with O157 LPS antibodies England: 73% (Chart, Lancet 1991) Central Europe: 73% (Bitzan, Epidemiol Infect 1993) France: 67% (Decludt, Epidemiol Infect 2000)
![Page 46: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/46.jpg)
Other studies in the United States and other countrieshave also reported that E. coli O157 is the major cause of HUS
![Page 47: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/47.jpg)
CDC work to improve diagnosis of STEC infections
Began a clinical diagnostics working group includes CDC, clinical labs, others Meetings
May 2006 January 2007
Published MMWR with guidelines, September 2006
![Page 48: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/48.jpg)
![Page 49: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/49.jpg)
Clinical laboratories should strongly consider including STEC O157 in their routine bacterial enteric panel
The best way to identify all STEC infections is to screen all stool samples…..for Shiga toxins
Laboratories that use a Shiga toxin EIA….should culture all positive broths….
When a Shiga toxin-positive broth does not yield STEC O157, the broth...should be quickly forwarded to the state…laboratory for identification of non-O157 STEC.
All non-O157 STEC…should be sent…to CDC.
![Page 50: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/50.jpg)
Summary: non-O157 STEC in the United States Non-O157 STEC are a diverse group
but ~75% of human infections are due to 6 serogroups
Clinical illness due to non-O157 STEC includes diarrhea, bloody diarrhea,
HUS less likely severe than E. coli O157
![Page 51: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/51.jpg)
Summary (continued) Most non-O157 STEC infections are not diagnosed
few clinical labs test stools for Shiga toxin but use of the EIA has increased
more non-O157 STEC illnesses and outbreaks detected Challenges in testing for STEC by EIA
“Shiga toxin positive” is not sufficient Serogrouping is important
Rapid identification of E. coli O157 is important for outbreak detection
![Page 52: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/52.jpg)
Summary (continued) STEC Diarrhea
O157 and non-O157 STEC isolated with similar frequency STEC-associated HUS
estimate <10% caused by non-O157 STECstrains that produce only Shiga toxin 1 much less
likely to cause HUS than strains that produce Shiga toxin 2
• 61% of human non-O157 STEC strains produced only Shiga toxin 1
![Page 53: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/53.jpg)
Contributors
State and local health departments Enteric Diseases Epidemiology Laboratory Many other collaborators
![Page 54: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/54.jpg)
Enteric Diseases Epidemiology Branch
![Page 55: CDC perspective on non-O157 Shiga toxin-producing E. coli (STEC) in the United States](https://reader035.fdocuments.us/reader035/viewer/2022062502/56815cab550346895dcaacc2/html5/thumbnails/55.jpg)
Thank you
Conclusions and opinions expressed herein are those of the presenter and do not necessarily represent the views and policies of CDC and DHHS.