Food and Health @ iBET

Catarina Duarte

Nutraceuticals and controlled Delivery Lab

IBET/ ITQB-UNL

Oeiras, Portugal

To create value for our partners leveraging scientific & technological knowledge in Biology and Chemistry

To push for faster utilization of the new biotechnological tools in business activities;

To provide joint research advanced training – OPEN INSTITUTION - Research conducted through a close partnership with ITQB

To provide a support infrastructure for academia & companies in biotechnological processes and manufacturing in Portugal;

To support start up/spin off companies at the incubation stage of their activities

Mission :

www.ibet.pt

Oeiras, Lisbon

BIAL │ IBERFAR │ JOSÉ de MELLO SAÚDE

GRUPO AZEVEDOS │ MEDINFAR │ TECNIMEDE

PHARMACEUTICALS AND HEALHCARE

AGROFOOD AND FORESTRY INDUSTRIES

AMORIM CORK INVESTIGAÇÃO E SERVIÇOS │ NUTRINVESTE

RAR│ SUMOL+COMPAL

ACADEMIC INSTITUTIONS

ESBUC – ESCOLA SUPERIOR DE BIOTECNOLOGIA (UCP)FCT-UNL – FACULDADE DE CIÊNCIAS E TECNOLOGIA (UNL)

ITQB – INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA (UNL)FACULDADE DE CIÊNCIAS MÉDICAS (UNL)

REITORIA DA UNIVERSIDADE NOVA DE LISBOA

FCT – FUNDAÇÃO PARA A CIÊNCIA E TECNOLOGIAFLAD – FUNDAÇÃO LUSO-AMERICANA PARA O DESENVOLVIMENTOIAPMEI – INSTITUTO DE APOIO ÀS PEQUENAS E MÉDIAS EMPRESAS

IICT – INSTITUTO DE INVESTIGAÇÃO CIENTÍFICA TROPICALINIAV – INST. NACIONAL DE INVESTIG. AGRÁRIA E VETERINÁRIA

FOUNDATIONS AND INSTITUTES

In total, iBET is 60% privately owned

Non-for-profit

Member of the LAO (ITQB, IGC, iBET, CEDOC)

16 fully equipped

laboratories

Pilot Plant

cGMP Analytical Services Unit

cGMP CMO (GeniBET)

Human resources: 18 PhDs

4 Engineers 67 Researchers

14 Lab Technicians

FLEXIBILITY & CONFIDENTIALITY

SPECIFIC NEEDS

• Contract Research Projects

• “Umbrella Research Contracts”

(plus per project based contracts)

• Satellite Laboratories

(Merck Serono and Bayer Healthcare)

• Joint Research for Advanced Training

(MSc, PhD, publications submitted after both parties approval)

iBET is a certified Service Provider

Agro-forestry

Biopharmaceuticals and novel therapies

cGMP Analytical services and Bioassays

Food in Health

Food, Water and Energy Nexus

Protein stability, structure and drug design

Bioactivity Chemical Characterization

and Metabolism

Process Tecnology

Analytical characterization:

Phytochemicals

Carbohydrates

Proteins

Sensory analysis

Metabolism:

• in vitro and in vivo digestion

and bioavailability

• metabolomic

Extraction and Fractionation (green and sustainable processes)

Fermentation

Formulation (development of bioactive and

bioavailable forms)

Scale-up (extraction, fermentation, drying)

Toxicity assessment

Health benefits (cell models, yeast models, …)

Preservative effects

Molecurar Structure - Bioactivy

Food and Health

Chemical Characterization and Metabolism

Analytical characterization:

Phytochemicals

Carbohydrates

Proteins

Sensory analysis

Metabolism:

• in vitro and in vivo digestion

and bioavailability

• metabolomic

Molecurar Structure - Bioactivy

Food and Health

Spectrophotometric assays

Chromatographic techniques

• Total polyphenols content

• Total flavonoids content

• Total phenolic acids content

• Total procyanidin content

• Total anthocyanin content

• Total betalain content

• Analysis of carotenoids

• TLC

• UPLC

•HPLC-DAD-FD-ED

• GC-MS

• FTIR

• NMR

Food and Health

Process Tecnology

• Conventional Extraction (with biocompatible solvents) • Pressurized Liquid Extraction • Supercritical Fluid Technology • Adsorption processes (molecular imprinted polymers)

Bioactive rich fractions (e.g. polyphenols, carotenoids, phytosterols, terpenes,...)

Natural colours

Natural flavours

Natural preservatives

Application in food, cosmetic or pharma industries

Extraction /Fraccionation of Natural Compounds

Food and Health

Process Tecnology

Materials Processing – particle formation, encapsulation and impregnation

Fundamental studies

Design & optimization of Delivery Systems

DDS Characterization

(morphology, particle size, pharmacokinetics,…)

Nanotoxicity

Supercritical fluid Technology

Solid material processing with

ScCO2

Particle formation technologies

Impregnation by adsorption

Molecular Imprinting

Spray drying

Spray chilling

Fluidized-bed spray

“Conventional” Techniques

Food and Health

Bioactivity

Characterization and pre-clinical Bioactivity evaluation of bioproducts

Enzymatic assays

Cell-based assays

Chemical assays

In vitro chemical and cell based assays for pre-clinical studies (antioxidant, anti-inflammatory, anti-proliferative,, neuroprotection, cardioprotective, anti-diabetes…)

Transport and permeability studies (in vitro)

Bioavailability evaluation after digestion (in vitro)

Molecurar Structure - Bioactivy

Catarina Duarte

Contacts:

cduarte@ibet.pt

http://www.ibet.pt/services/food-health