CATALOG OF ANTIBODIES FOR MARKERS - Novus Biologicalsimages.novusbio.com/design/markers.pdf ·...

For research purposes only.Not for use in humans.Prices subject to change. FAX: 303.730.1966 • WEB: www.novusbio.com 2

MARKERSCATALOG OF ANTIBODIES FOR

Stem Cell • Neural • Fibroblast • Organelle • Cancer

Daily product updates!www.novusbio.comTOLL FREE: 888.506.6887 • PHONE: 303.730.1950

Table of ContentsStem Cell Markers................ 2-5

Neural Markers ..................6-12

Organelle Cell Markers ....12-16

Cancer Markers ...............17-22

Application KeyELISA - Elisa

FACS - Fluorescent Activated Cell Sorting

ICC - Immunocytochemistry

IF - Immunofluorescence

IHC - Immunohistochemistry

IHC-Fr - Immunohistochemistry Frozen

IHC-P - Immunohistochemistry Paraffin

IP - Immunoprecipitation

RIA - Radioimmunoassay

WB - Western Blot

Reactivity KeyAm - Amphibian

Av - Avian

Bb - Baboon

Bv - Bovine

Ca - Canine

Ch - Chicken

Dr - Drosophila

Eq - Equine

Fe - Feline

Gp - Guinea Pig

Gt - Goat

Marker Antibodies

1

Ha - Hamster

Hu - Human

Ma - Mammal

Mk - Monkey

Mu - Mouse

Po - Porcine

Rb - Rabbit

Rt - Rat

Ye - Yeast

Xp - Xenopus

Hematopoietic Stem Cell .............................. 2-5

Mesenchymal Stem Cell ....................................2

Embryonic Stem Cell .................................... 2-5

Neuronal Stem Cell ..........................................3

Keratinocyte Stem Cell ......................................3

Hemangioblast Stem Cell ..................................5

Astrocyte/Astroglia Markers ...............................6

General Glial Cell Markers................................7

Microglia Markers .............................................7

Neuronal Markers ......................................8-10

Oligodendrocyte Markers ..........................10-11

Schwann Cell Markers.................................... 11

Stem Cell Marker Antibodies

Fibroblast Marker Antibodies .....................6

Neural Cell Marker Antibodies

Autophagic Vesicles Markers ........................... 12

Centrosome Markers ...................................... 12

Endosome Marker.......................................... 13Endoplasmic Reticulum Markers ..............................13Golgi Complex .............................................. 14

Lysosome Markers ......................................... 15

Mitochondrial Markers ............................15-16

Nuclear Markers ............................................ 16

Organelle Cell Marker Antibodies

Breast Cancer ..........................................17-19

Colorectal Cancer ....................................19-20

Lung Cancer ...........................................20-21

Prostate Cancer .............................................. 22

Cancer Marker Antibodies

Musashi 1

GFAP

Beta Tubulin 3

UCHL1


Stem Cell Markers

CD105 Antibody Mesenchymal Stem Cell MarkerNB600-1011

Species: MuApplications: FACS, IHC-Fr, IP, WB

CD133 AntibodyHematopoietic Stem Cell MarkerNB120-16518


Species: Hu, MkApplications: IHC, IP, WB

Staining of humantonsil using NB100-1934.

ABCG2 Antibody Hematopoietic Stem Cell MarkerNB100-2177

Species: HuApplications: ICC, IHC-Fr, IHC-P, WB

ABCG2 (ATP-binding cassette superfamily G member 2) is a hematopoietic stem cell marker that is found in a wide variety of stem cells.


Species: Hu, MkApplications: FACS

Connexin 43 AntibodyNB300-309

Species: Hu, Mu, Mk, RtApplications: WB

Detection of Connexin 43 in rat cerebellar lysate using NB300-309.

CD34 AntibodyHematopoietic Stem Cell Marker NB600-1071

Species: MuApplications: FACS, IHC, IHC-P, IP, WB

Staining of murine hair follicles using NB600-1071.

CRIPTO AntibodyEmbryonic Stem Cell MarkerNB100-1597

Species: HuApplications: WB

Detection of Cripto in MDA-MB21 lysate using NB100-1597

CRIPTO AntibodyEmbryonic Stem Cell MarkerNB100-1598

Detection of Cripto in MDA-MB21 lysate using NB100-1598.

Connexin 43 Antibody NB100-722

Species: Hu, Mu, Mk, RtApplications: WB

Detection of Connexin 43 in rat hippocampal lysate (showing specifity, lane 1) and lambda-phosphatase incubated rat hippocampal lysate (negative control, lane 2) using NB100-722.

Species: Hu, MuApplications: ELISA, ICC, IF, IHC, IHC-P, WB

CD133 is a cell-surface protein that identifies neural stem cells, which give rise to neurons and glial cells. This antibody is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelia.

CD117 is expressed on the surface of hematopoietic stem cells as well as other cell types. This receptor binds to stem cell factors.

This 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.


Functionally, stem cells are able to propagate and generate additional stem cells, and can differentiate into various progenitor cells which further maturate along specific lineages. Stem cell markers allow for the identification and isolation of stem cells in different types of tissues and cell populations. Such markers allow identification of embryonic, hematopoietic, mesenchymal/stromal, and neural stem cells. Embryonic stem (ES) cells, derived from the inner cell mass of preimplantation embryos, are recognized as

the most pluripotent population, having the greatest developmental potential. However, for many applications adult stem cells may be preferred due to ethical issues involved in the derivation of ES cells. Stem cell research is poised to allow the development of new therapies through repair, replacement, and regeneration of damaged or diseased cells and tissues making it one of the most exciting frontiers in modern medicine.

The cripto gene is expressed both in embryonic stem cells and during the early phases of embryo development, while, in the adult, it is reactivated in a wide range of epithelial cancers.

The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.

Connexin 43 is a negative cell surface marker for the stem cell-containing population of human limbal epithelial cells. Connexin 43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy.


NB100-56450Detection of Nanogin (A) HeLa and (B) NIH/3T3 cells usingNB100-56450.

Species: Hu, Mu, Mk, GtApplications: WB

NB100-588

Species: MuApplications: IP, WB

Detection of Nanog in mouseembryonic stem cellsusing NB100-588.

NB100-58842

Species: MuApplications: IHC

Staining of mouse teratoma usingNB100-58846.

Nestin AntibodyNeuronal Stem Cell MarkerNB100-1604

Species: MuApplications: ICC, IHC

Staining ofneural stem cells of the ventricular zone of the CNS usingNB100-1604.

NB100-59737

Species: HuApplications: ELISA, WB

Detection of Nanog in HeLa nuclear lysates using NB100-59737.


Detection of nestin in human fetal temporal lobe tissue lysates using NB300-265. Lane 1: 0.5 ug/mlLane 2: 2 ug/ml

Nanog is a molecular marker that recognizes the undifferentiated state of stem cells in mouse and human. It is a divergent homeodomain protein that directs propagation of undifferentiated ES cells. Nanog-deficient embryonic stem cells lose pluripotency and differentiate into extraembryonic endo-derm lineage.


p63 AntibodyKeratinocyte Stem Cell MarkerNB110-57175

Staining of paraffin-embedded human breast carcinoma using NB110-57175.

Species: Hu, Mu, RtApplications: FACS, IHC, WB

Podocalyxin AntibodyHematopoietic Stem Cell MarkerNB110-41503


Detection of podocalyxin in human lung lysate using NB110-41503.

p63 Antibody Keratinocyte Stem Cell MarkerNB110-57309

Species: HuApplications: ICC, IP, WB

Immunofluorescent staining of A431 cells using NB110-57309.

PSCA AntibodyProstate Stem Cell MarkerNB120-15168

Species: HuApplications: IHC-P

Podocalyxin AntibodyHematopoietic Stem Cell MarkerNB110-41510


Detection of podocalyxin in human lung lysate using NB110-41510.

NB110-40414


Detection of Nanog in human hippocampus tissue lysate usingNB110-40414.

Musashi-1 AntibodyNeuronal Stem Cell MarkerNB100-1759

Species: Hu, Mu, RtApplications: ICC, IHC, IHC-P, WB

Staining of neural rosettes using NB100-1759.

Keratin 19 AntibodyPancreatic Stem Cell MarkerNB110-56914

Species: HuApplications: IHC, ICC, FACS, IP, WB

Staining of HeLa cells using NB110-56914.

Staining of human breast cancer usingNB120-15168.

Nestin is a Class VI intermediate filament expressed in the developing CNS in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells.

The p63 gene is highly expressed in the basal or progenitor layers of many epithelial tissues. p63 plays a fundamental role associated with development and differentiation.

Podocalyxin is a CD34 family member expressed by podocytes, vascular endothelia, mesothelia, and a subset of hematopoietic progenitors. Podocalyxin appears to complement CD34 as a useful cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

CK19 is synthesized mainly in embryonic and adult simple epithelia, but has also been found in stratified epithelia as well.

Musashi-1 is important for cell fate determination, with a role in maintenance of the stem cell state, differentiation and tumorigenesis.

NANOG AntibodiesEmbryonic Stem Cell Markers


OCT4 AntibodyEmbryonic Stem Cell MarkerNB100-41088


Detection of OCT4 in human liver lysateusing NB100-41088.

OCT4 AntibodyEmbryonic Stem Cell MarkerNB100-2379


Detection of OCT4 in HeLa whole cell extract using NB 100-2379.

Nucleostemin AntibodyEmbryonic Stem Cell MarkerNB100-1569

Species: Hu, MuApplications: IP, WB

Detection of human and mouse GNL3 in HeLa, 293T and 3T3 cells using NB100-1569.



Detection of human and mouse GNL3 in HeLa, 293T and 3T3 cells using NB100-1568.


Species: Hu, MuApplications: IHC

Staining of human skin basal cells carcinomausing NB100-2896.

Sox2 AntibodyEmbryonic Stem Cell MarkerNB110-37235

Species: MuApplications: IHC, WB

Detection of SOX2 in mouse brain lysate using NB110-37235.

Sca-2 AntibodyHematopoietic Stem Cell MarkerNB100-58797

Species: HuApplications: IHC-P, IP, WB

Staining of cerebellar Purkinje neurons using NB100-58797.

SSEA AntibodiesEmbryonic Stem Cell Markers

Species: Hu, MuApplications: FACS, ICC, IF

SSEA-1 is expressed on the surface of preimplantation-stage murine embryos and has been found on the surface of teratocarcinoma stem cells, but not on their differentiated derivatives. SSEA-3 is synthesized during oogenesis and is present in the membranes of oocytes, zygotes and early cleavage-stage embryos. Biological roles of these carbohydrate-associated molecules have been suggested in controlling cell surface interactions during development.

Undifferentiated primate ES cells, human EC and ES cells express SSEA-3, but not SSEA-1. Undifferentiated mouse ES cells express SSEA-1, but not SSEA-3. SSEA-1 staining has been seen in the the oviduct epithelium, endometrium, epididymis, and in some areas of the brain and kidney tubules in adult mice.

NB100-297

Species: HuApplications: IF, WB

Detection of hTERT in MJ90 whole cell lysate using NB100-297.

SSEA3 AntibodyNB100-1832Species: HuApplications: FACS, IF, IHC

NB100-317

Species: HuApplications: FACS, IF, WB

Staining of normalpancreas using NB100-317.

NB120-32020

Species: HuApplications: FACS, ICC, IHC, WB

Staining of paraffin-embedded human lung adenocarcinoma using NB120-32020.

Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow, and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells.

The transcription factor OCT4, expressed in ES cells and germ cells, is strongly implicated in the process of maintaining as well as regaining stem-cell pluripotency and functions as a key regulator of mammalian germline development. It is expressed in totipotent embryonic stem and germ cells. A critical level of Oct-4 expression is required to sustain stem cell self-renewal and pluripotency.

The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord.

The SOX (SRY-box containing gene) gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate.

Telomerase AntibodiesEmbryonic Stem Cell Markers

SSEA1 AntibodyNB100-1831

Telomeres are nucleoprotein structures at the ends of eukaryotic chromosomes.Telomerase is an enzyme uniquelyassociated with immortal cell lines andis useful for identifying undifferentiated PSCs.


Thrombopoietin AntibodyHematopoietic Stem Cell MarkerNB110-68129

Detection of TPO Lane 1: 100ng mouse TPO (non-reduced) Lane 2: 100ng mouse TPO (reduced).

Species: MuApplications: ELISA, WB

TRA 1-60 AntibodyEmbryonic Stem Cell MarkerNB100-730

Species: HuApplications: FACS, IF, IHC, IP, WB

NB100-686

Species: Hu, Mu, RtApplications: IF, IHC-Fr, IHC-P, WB

Staining ofFFPE human angiosarcomausing NB100-686.

TRA 1-81 Antibody Embryonic Stem Cell MarkerNB100-1833

Species: HuApplications: FACS, IF, IHC, IP, WB

Immunofluorescence staining of methanol-fixed NTERA-2 cl.D1 cells showing membrane localization usingNB100-1833.

NB600-1433

Species: Hu, Mu, RtApplications: IHC-P, WB

Staining of formalin fixed paraffin embedded human angiosarcoma using NB600-1433.

NB100-527

Detection of VEGFR2 in ChimericCSF-1R/VEGFR2 using NB100-527.

NB200-208

Species: Hu, RtApplications: ELISA, FACS, IHC

Staining of myocardium section using NB200-208. The arrowheadsindicate VEGFR-2 positive endothelial cells from myocardial capillaries.


NB100-530


Detection of VEGFR2 in VEGFR2 induced HUVEC lysate using NB100-530.

NB110-55547

Species: HuApplications: IHC

Staining ofbreast carcinomausing NB110-55547.

NB100-529


Detection of VEGFR-2 in CSF-1 receptor/VEGFR2 chimera transfected lysate using NB100-529.

NB100-627


Detection of VEGFR2 in CSF-1/VEGFR2 transfected lysates using NB100-627. Lane 1 - 1:2,000Lane 2 - 1:10,000

TRA-1-81 and TRA-1-60 are cell-surface proteins involved in cell differentiation. Cell surface antigens are powerful tools for character-izing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis.

NB110-55548


Staining of breast carcinoma usingNB110-55548.

Thrombopoietin (TPO) has been shown to function both as a proliferative and differentiative factor for megakaryocytes and as a survival and weakly proliferative factor for hematopoietic stem cells.

Receptors for Vascular Endothelial Growth Factor (VEGF), VEGFB and PGF have a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.

VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis.

VEGFR2 AntibodiesHemangioblast/Stem Cell Markers

NB100-2382Detection of VEGFR2 doublet in CSF-IR/VEGFR2 chimera transfected lysate using NB100-2382.


NB110-57149Detection of VEGFR2in ECV304 cell lysate using NB110-57149.


NB110-57644Detection of VEGFR2in ECV304 cell lysateusing NB110-57644.


Detection of TRA1-60 in NTERA-2 cell lysate using NB100-730.


Neural Cell Markers

GFAP AntibodyNB300-141

alpha Smooth Muscle Actin Antibody NB120-18460

Species: Bv, Ch, Hu, Ma, Mu, Rt, BbApplications: IF, IHC-Fr, IHC-P, WB

Fibroblasts (Pan Reticular) AntibodyBM4018

Species: Hu, MuApplications: IHC-Fr

Staining ofreticular fibroblasts and reticular fibres with BM4018 antibody.

Fibroblast (Surface Protein) AntibodySM1214P

GLAST AntibodyNB100-1869

Species: RtApplications: FACS, ICC, IHC, WB

Species: Bv, Hu, Mu, Po, Rt, FeApplications: ICC, IF, IHC, IHC-Fr, IHC-P, WB

Species: HuApplications: FACS, IHC, IP

Staining of human leiomyosarcoma us-ing NB120-18460.

Staining of typical astrocytic cells using NB 300-141 in neurons and glia of rat forebrain.

GLAST expression begins in the newborn and persists throughout life. This consistency and the fact that GLAST expression is linked to a func-tional role of astrocytes in glutamate homeostasis makes it a good marker for astrocyte identification.

Astrocyte/Astroglia Markers

Fibroblast Markers

Astrocytes are star-shaped glial cells that perform multiple functions in the CNS. They play a significant role in maintaining normal brain physiology during development and in adulthood. Astrocytes also provide

physical support to neurons, clean up debris within the brain, provide chemicals to help control fluid composition surrounding neurons, and provide nutritional support to neurons.

GFAP AntibodyNB300-142

Species: Hu, MaApplications: IF, WB

Rat cortical neurons and glia in mixed tissue culture stained with Chicken polyclonal to MAP2 - NB300-213 (green) at 1/30000 and Mouse monoclonal to GFAP - NB300-142 (red) at 1/100.

Smooth muscle vessels alpha actin has been found in distinct populations of astrocytes in the adult mouse brain, in particular in the corpus callosum and the fornix. However, in the corpus callosum, it is seen only in parasagittal planes. Thus, alpha smooth muscle actin represents a new marker for subsets of astrocytes.

ABCA1 AntibodyNB400-105

Species: Ha, Hu, Mu, RtApplications: FACS, ICC, IF, IHC-P, IP, WB

Staining of prostate epithelium using NB400-105.

In the brain, ABCA1 is expressed in neurons, astrocytes, and microglia and is induced by liver X receptor (LXR) and retinoid X receptor (RXR) agonists.

Survivin AntibodyNB500-201

Species: Hu, Mu, Ca, Rt, FeApplications: IF, WB, IHC-P, IP, ICC.

Telophase with accumulation of survivin in the mid-bodies of two daughter cells.

Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury.

Staining of KG1 cells with mouse anti human fibroblast antibody (SM1214P).

Prolyl 4-Hydroxylase AntibodyAF5110

Species: RtApplications: ELISA, IHC, WB

Staining of frozen rat skin sections using AF5110.

Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumors, presumably derived from astrocytic cells, heavily express GFAP.


Microglia are the smallest glial cells that function similarly to macrophages in the blood system. They are the first immune defense in the CNS that seek and destroy damaged neurons, plaques, and infectious agents. When infections cross the blood-brain barrier, microglia cells act to increase inflammation in order to destroy the infectious agents before they cause damage to the brain and/or spinal cord. Due to their plasticity, microglia cells are present in various forms depending on their location in the body, such as ameboid, ramified, phagocytic, juxtavascular, and gitter cells.

Sox2 Antibody NB110-37235


Vimentin AntibodyNB300-223

Species: Hu, Mu, RtApplications: IF

Vimentin (phospho Ser38) AntibodyNB110-57646

Species: Hu, Mu, RtApplications: WB

Detection of Vimentin in 1) untreated and 2) Calyculin A treated HeLa cell lysate using NB110-57646.


Species: Hu, Mu, RtApplications: IHC, IP, WB

Staining of paraffin-embedded human melanoma usingNB110-57645.


Species: AllApplications: IHC-P, IP, WB

Staining of formalin-fixed, paraffin-embedded human osteosarcoma using NB200-622.

Staining of humanuterus using NB110-37235.

Rat glial cells in mixed cerebral cortex cultures stained with anti-vimentin (NB 300-223)[green] and counter-stained with anti-GFAP (NB300-142) [red]. Hoechst dye reveals nuclear DNA [blue].

General Glial Cell Markers

Vimentin (Phospho Ser72) AntibodyNB110-57647

Detection of Vimentin in 1) untreated and 2) Calyculin A treated HeLa cell lysate using NB110-57647.

Species: Hu, Mu, RtApplications: IP, WB

Vimentin (Phospho Ser82) Antibody NB110-57648


Detection of Vimentin in 1) AP treated and 2) untreated HeLa + Calyculin A cell lysate using NB110-57648.

Microglia MarkersCD11 b/c AntibodyNB110-40766

Species: Hu, MuApplications: IHC, IHC-P, WB

Staining of bone marrow, myeloid precursors usingNB110-40766.

Ferritin Antibody NB600-920

Species: HuApplications: ELISA, IHC, IP, WB

Detection ofFerritin in HT1080 cells using NB600-920using IP.

Glial cells are non-neuronal cells that include microglia and macroglia cells. The latter includes astrocytes, oligodendrocytes, ependymal cells, radial glia, Schwann cells and satellite cells. Glial cells, which are

often referred to as the glue of the CNS, function to support and protect neurons, maintain homeostasis, form myelin, and partake in signaling in the central nervous system (CNS).

Detection of SOX2 in mouse brain lysate using NB110-37235 .

Sox2 Antibody NB110-37235


The SOX (SRY-box containing gene) gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate, and are expressed in the Bergmann glia, a population of radial glia present in the Purkinje cell layer.

Vimentin is the 10nm or intermediate filament protein subunit found in many kinds of mesenchymal and epithelia cells, in many kinds of cells in tissue culture, and in developing neuronal and astrocytic precursor cells in the central nervous system. Vimentin frequently forms co-polymers with other intermediate filament proteins, such as GFAP (in astrocytes), with desmin (in muscle cells) and neurofilamentproteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and are generally used to reveal the filamentous cytoskeleton.

CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.

Ferritin is a a marker for microglia, especially for reactive microglia and macrophages.

10For research purposes only.Not for use in humans.Prices subject to change. FAX: 303.730.1966 • WEB: www.novusbio.com

Calretinin AntibodyNB300-707

Detection of Calretinin rat brain homogenates usingNB300-707.

Species: Hu, Mu, RtApplications: IP, WB

c-Fos Antibody Neuronal ActivationNB600-904

Species: Hu, Mu, RtApplications: IHC-P, IP, WB

Staining of rat spinal cord uisng NB600-904.


Species: Hu, Mu, RtApplications: IHC

Staining of paraffin-embedded Human mesothelioma usingNB110-55651.


Species: Hu, Mu, Rt, AmApplications: IHC-P

Staining of human mesothelioma using NB200-618. Note cytoplasmic and nuclear staining of tumor cells.

Beta Tubulin 3 AntibodyDendrite MarkerNB100-1612


Dissociated cell culture of a neonatal mouse brain showing beta Tubulin 3 (green staining) of neurons.

Neuronal Markers

Acetylcholinesterase AntibodyEarly Neuronal DevelopmentNB100-1519

Species: Hu, Mu Applications: ELISA, WB

Detection of ACHE in A431 lysate usingNB100-1519.

Bcl-2 AntibodyNeuronal Apoptosis InhibitorNB110-55551

Species: Hu, MuApplications: FACS, ICC, IHC, IP, WB


Bag 1 AntibodyNeuronal DifferentiationNB110-55489

Species: Hu, RtApplications: ICC, IHC

Staining of paraffin-embedded colon carcinoma using NB110-55489.


Staining of human tonsil using NB200-572. Note absence of staining of reactive cells in the germinal center.

Species: Hu, MkApplications: IHC-P, WB


Species: Hu, Mu, RtApplications: ELISA, WB

Detection of Bcl-2 in human lymph node lysate using NB300-900.

Bcl-2 AntibodyNeuronal Apoptosis Inhibitor NB100-2087


Detection of Bcl-2 in human lung cell extract using NB100-2087.

Neurons, the functional unit of the nervous system, are electrically excitable cells that transmit signals through-out the body. Generally composed of a soma, dendritic

tree, and axon, their size, shape, and function varies greatly.

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells. The broad range of protective effects of BCL-2 protein suggests that BCL-2 may interact with a central step in neural cell death.

Acetylcholinesterase, or AChE, hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission

Bag1 is a multifunctional protein that blocks apoptosis and interacts with several types of proteins, including the Bcl 2 family proteins.

Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development.

The Fos proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. Fos was the first transcription factor identified with a critical function in regulating the development of skeletal cells.

Mesothelial Cell Marker: Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue and is highly expressed in auditory neurons. It has been implicated in a calcium buffer, calcium sensor, and as a regulator of apoptosis. Calretinin is considered a specific marker of mesothelial cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas. It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma.

c-Fos AntibodyNB120-14285

Species: Bv, Hu, Mu, RtApplications: WB

Detection of c-Fos inwestern blot.


ChAT Antibody Cholinergic Neuron MarkerNB100-40794

Species: Rt, MuApplications: ELISA, WB

ELF AntibodyNeuronal Precursor Cell MarkerNB100-55272


Staining of humanprostate adenocarcinomausing NB100-55272.


Detection of Human ELF1 in HeLa whole cell lysates using NB100-68253.


Species: Hu, MuApplications: WB, IP

Detection of SPTBN1 in HeLa and 293T cells using NB100-40828.


Neurofilament Light AntibodyNB300-133

Species: Bv, Hu, Ma, Mu, Po, Rt, Fe, Av Applications: ICC, IF, IHC-Fr, IHC-P, WB

Detection of NF-L in whole rat cerebellum homogenate usingNB300-133.

Neurofilament Heavy AntibodyNB300-135

Species: Hu, Ma, Mu, MkApplications: IHC-Fr, IHC-P, WB

Section of rat spinal cord stained with NB300-135 (green) and NB110-58869 (red).

NQO1 AntibodyNeuronal Injury MarkerNB200-209

Species: Ca, Hu, Mk, RtApplications: IF, IHC, IP, WB

Detection of NQO1 in A549 lysates using NB200-209. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml.

MAP2 AntibodyNeuronal Dendrite MarkerNB300-213

Species: Bv, Hu, Mu, RtApplications: IF, WB

Rat neurons stained with anti-MAP2 (NB300-213) [green] and anti-GFAP (NB300-142) [red].

HSV-1 AntibodyNeuronal Latency MarkerNB110-57251

Species: AllApplications: ELISA, IF, WB

10-well HSV-1 slides, 10-well HSV-2 slides, anti-Mouse-FITC F(ab)2 with counterstain.

Detection of ChAT in mouse brain lysate using NB100-40794.


Species: HuApplications: ICC, IHC, IF, WB

Visualization of nestin in PC-3 cells using NB300-266. Courtesy of Dolnick Lab, Roswell Park Cancer Institute.

Neurofilament Medium AntibodyNB300-134

Culture of adult neural cells. Mature neurons can be identified by morphology and staining.

Species: Hu, Ma, Mu, AvApplications: IHC, WB

ChAT Antibody Cholinergic Neuron MarkerR-043-100

Species: Hu, Rt, Rb, GpApplications: ELISA, IHC, WB

Staining of rat facial nucleus using Rabbit antibody to ChAT (R-043-100) at 1:2000 dilution.

ChAT serves as a specific marker for cholinergic neurons in both peripheral and central nervous systems. It is also present at parasympathetic postganglionic fibres.

Spectrins play a pivotal role in axonal transport, neurite extension, the organization of synaptic vesicles, as well as for protein sorting in the Golgi apparatus and cell membrane.

Following primary infection in humans, herpes simplex virus type 1 (HSV-1) establishes latent infections in sensory neurons.

Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. Antibodies to MAP2 are excellent markers on neuronal cells, their perikarya and neuronal dendrites.

Neuronal damage is associated with an increased activity of NQO1.

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells.

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L runs on SDS-PAGE gels at about 68kDa; NF-M at 145-170 kDa; NF-H at 180-220 kDa. Antibodies to the neurofilaments are useful for identifying neuronal cells and their processes in tissue sections, and can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig’s disease or Alzheimer’s disease.


CNPase AntibodyNB100-1935


Adult mouse brain showing CNPase (brown staining)in white matter tracts and the granule cell layer of the cerebellum.

Amphoterin AntibodyNB100-2322

Species: Hu, MuApplications: WB

Detection of HMGB1 in whole cell HeLa lysate using NB 100-2322.

CNPase AntibodyNB110-79874


Oligodendrocyte Markers

PGP9.5 AntibodyNB110-58874

Species: Hu, RtApplications: IF, WB

Shows rat mixed neuron/glial cultures stained with Rabbit UCHL1 (green). Blue is a DNA stain.

Synapsin 1 AntibodyNB300-104

Species: Hu, Mu, RtApplications: IF, IHC, IP, WB

Detection of synapsin 1 in rat hippocampal lysate using NB300-104.

Synapsin 1 AntibodyNB300-312

Detection of synapsin 1 in rat hippocampal lysate using NB300-312.



Species: Mu, HuApplications: IHC, WB

Staining of human brain using NB300-675.


Staining of rat abdominal tissue using NB600-1160.

Species: Hu, RtApplications: ELISA, IHC-P, WB

UCLH1 Antibody NB110-58872


Shows rat mixed neuron/glial cultures stained with chicken UCHL1 (green) and rabbit antibody to glial fibrillary acidic protein (GFAP-red), NB300-141. Blue is a DNA stain.

Oligodendrocytes provide support to axons and produce insulating myelin sheaths. The CNS depends on this insulation to reduce ion leakage in order to increase overall nerve conduction speed. When myelin

sheaths are degenerated oligodendrocytes attempt to replace them, however, in multiple sclerosis patients oligodendrocytes themselves are destroyed thus compromising their ability to perform repairs.

PGP9.5/UCHL1 AntibodiesPeripheral Nerve Fiber Markers

Ubiquitin C-terminal hydrolase 1 (UCHL1) was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5. The protein is extremely abundant representing 1-2% of total brain protein. It was later found that a ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein, resulting in the renaming of PGP9.5 to ubiquitin C-terminal hydrolase 1.

Since UCHL1 is heavily expressed in neurons, antibodies to UCHL1 can be used to identify neurons in histological sections and in tissue culture. The great abundance of this protein in neurons means that it is released from neurons in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker. UCHL1 was also discovered as a gene mutated in some rare famial forms of Parkinson’s disease. Interestingly, a common allelic variant of UCHL1, the S18Y polymorphism is actually protective against Parkinson’s disease.

Synapsin I plays a key role in synaptic plasticity in the brain. This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation. Thus Synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation.

Detection of CNPase in normal brain tissue lysate using NB110-79874.

The onset of amphoterin expression, prior to the expression of myelin-specific anti-gens, makes amphoterin a useful marker for studying oligodendrocyte development.

2’,3’-cyclic nucleotide 3’-phosphodiesterase (CNPase) is associated with brain tubulin and acts as a microtubule-associated protein in promoting microtubule assembly and distribution. CNPase is used as a marker of cell differentiation in the CNS as it is ex-pressed primarily in committed oligodendroglial progenitors and terminally-differentiated oligodendrocytes.


MAP-4 AntibodyNB100-74615

Species: Hu Applications: IP, WB

Detection of human MAP4 in HeLa cell lysate using NB100-74615.

MOG AntibodyNB300-948

Detection of MOG in human brain lysate using NB300-948.

Species: Hu, Mu, RtApplications: ELISA, WB

Olig2 AntibodyNB110-41440

Species: Hu, MuApplications: WB

Detection of Olig 2 in Jurkat whole cell lysates using NB110-41440.

Nestin AntibodyNB100-1604


AN2 AntibodyNB300-750

Detection of PAX6 in rat whole eye extract using NB 300-750.

Species: Mu, Hu, Ch, Xp, Rt, ShApplications: WB

Laminin AntibodyNB300-144

Species: Hu, Mu, RtApplications: IHC-Fr, IHC-P, WB

Staining of rat spinal cord and dorsal root tissue using NB 300-144. Pepsin antigen retrieval was performed.

AN2 AntibodyNB100-61654


Detection of AN2 in mouse eye lysate usingNB100-61654.

Nestin AntibodyNB600-876

Species: MuApplications: WB

Detection of Nestinin MEF whole celllysates usingNB600-876.

Schwann Cell Markers

Ran-1 AntibodyNB100-1384

Species: Hu, Mu Applications: ELISA, WB

Detection of Ran-1in 3T3 cell lysate us-ing NB100-1384.

SCIP AntibodyNB100-1359

Detection of SCIPin human brainlysate usingNB100-1359.


Schwann cells are a type of glial cell that function as support cells for the PNS, similar to oligodendrocytes of the CNS. They mainly provide myelin insulation to

peripheral axons in order to increase signal conduction. Schwann cells also aid in clearing debris and guide axon regrowth in the PNS.

A tissue section of mouse brain showing Nestin (green staining) in neural stem cells of the ventricular zone of the CNS.

Microtubule-associated protein 4 is a major non-neuronal microtubule-associated protein and is a marker for astroglia and oligodendroglia cells.

Myelin-Oligodendrocyte Glycoprotein (MOG) is a surface marker of oligodendrocyte maturation.

The oligodendroglial lineage marker, Olig2, is expressed in neural progenitors and oligodendroglia and is essential for oligodendrocyte development.

Nestin is a marker of oligodendrocyte lineage cells. Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors.

AN2 is expressed by immature Schwann cells in vitro and in vivo and downregulated as the cells upregulate myelin genes.

Ran-1 is a marker for normal Schwann cells and is associated with adult non-myelin-forming Schwann cells.

SCIP, also known as Tst-1 or Oct-6, is expressed in proliferating Schwann cells in the PNS and O2A progenitor cells in the developing CNS.


Detection of laminin in EHS (1) and heart (2) lysatesusing NB300-144.


Laminin is a marker for human Schwann cells in vitro. Laminin antibodies are widely used to label blood vessels and basement membranes.


Organelle Markers

APG8b AntibodyR-155-100

APG8c AntibodyR-140-100

Species: HuApplications: IF, IHC, WB

Confocal microscopy on immunofluorescently detected MAP1LC3 C in cytospin-isolated human white blood cells.

APG8a Antibody R-146-100

Species: Hu, RtApplications: IF, IHC, WB

LC3 AntibodyNB100-2331

Species: Hu, MuApplications: IHC, IHC-P, IP, WB

LC3B AntibodyNB600-1384

Species: Hu, MuApplications: ICC, IF, IHC, WB

Staining of treated U373-MG (human glioblastoma) cells using NB600-1384.

LC3 Antibody NB100-2220

Species: Hu, Mu, RtApplications: IHC, IHC-P, IP, WB

Staining of brain, cerebral cortex, neuronsusing NB100-2220.

Pericentrin/Kendrin AntibodyNB100-61071

Species: HuApplications: IP

Detection of Human Pericentrin/Kendrin in HeLa cell extracts using NB100-61071.


Detection of Human Pericentrin/Kendrin in HeLa cell extracts using NB100-61072.

gamma Tubulin AntibodyNB100-1628

Species: Bv, Ca, Ch, Ha, Hu, Mu, Rt, XpApplications: ELISA, IHC, WB

Species: Hu, RtApplications: IF, IHC, WB

Species: HuApplications: IP, WB

Confocal microscopy on immunofluorescently detected MAP1LC3 A in cytospin-isolated human white blood cells.

Detection of APG8bin Olfactory cell line(Odora) lysate usingR-155-100.

Autophagic Vesicle Markers

Staining of brain, cerebral cortex, cell processes in gray matter usingNB100-2331

Centrosome Markers

Gamma tubulin is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome.


Species: HuApplications: ICC

Staining of HeLacells using NB100-68277.

The centrosome is the main microtubule organizing center (MTOC) of the cell and acts as a regulator of cell-cycle progression. Centrosomes are present in higher eukaryotic cells, but not plant cells. The centrosome is composed of two centrioles surrounded by an amorphous mass of pericentriolar material

(PCM). The PCM contains proteins responsible for microtubule nucleation and anchoring. Each centriole is composed of nine triplet microtubule blades in a pinwheel structure. Cancer cells often have an abnormal number of centrosomes, which may be of biological significance.

Autophagy is an evolutionarily conserved process responsible for the bulk degradation of intracellular material. In autophagy, cytoplasmic components are engulfed by double-membrane-bound structures

(autophagosomes) and delivered to lysosomes/vacuoles for degradation. Recent reports indicate that autophagy plays a crucial role in many different pathologies.

LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.

Pericentrin binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled-coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with gamma-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell-cycle progression.

Detection of gammaTubulin in chickenfibroblasts usingNB100-1628.


Endosomes are membrane-bound vesicles that are formed by endocytosis. Endocytosis involves invagination of the plasma membrane to encircle a substance and budding to release the vesicle. Endosomes function to either transport their contents to a lysosome for degradation or to transport substances between different portions of the external cell membrane, the latter of which is essential for epithelial cells whose polarity differs on each side of the membrane.

Endosome Marker EEA1 AntibodyNB300-502

Species: Ca, Ha, Hu, Mu, Rt, XpApplications: IF, IP, WB


Endoplasmic Reticulum Markers

Calreticulin AntibodyNB600-101

WFS1 Antibody NB100-1918

Species: Hu, Mu, RtApplications: IF, IHC-Fr, IP, WB

ERp29 AntibodyNB300-523

Species: Bv, Ca, Gp, Ha, Hu, Mu, Mk, Rt Applications: IF, IP, WB

ERp57 AntibodyNB100-1143


Detection of ERp57 in HepG2 lysate usingNB100-1143.

Calreticulin Antibody NB600-103


Detection of calreticulin in human kidney lysate using NB 600-103.

Species: Bv, Ha, Hu, Mu, RtApplications: ICC, IF, IHC, IP, WB

Immunofluorescence staining of Calreticulin in HCT15 colon cancer cells using NB 600-101.

Staining ofrat thyrocytes using NB 300-523.

GRP94 AntibodyNB100-41095

Heme oxygenase-1 AntibodyNB110-57028

Species: Hu, MuApplications: ICC, IHC, IP, WB

Immunofluorescent staining of HeLa cells using NB110-57028.

GRP78/BiP Antibody NB300-520

Species: Ha, Rt, MuApplications: IF, IP, WB


Detection of GRP78in liver, J77, CHQ and Akt-29 cellsusing NB300-520.

The endoplasmic reticulum is an organelle of interconnected tubules, vesicles, and cisternae. Its specific functions involve protein translation and folding,

transport of proteins to the cell membrane, secretion of cellular proteins, sequestration of calcium, and production and storage of macromolecules.

Detection of GRP94 in 3T3 lysate usingNB100-41095.

Immunoprecipitation of WFS1 using NB 100-1918. Precipitated lysateis INS-1.

PDI Antibody NB100-1921

Species: RtApplications: ICC, IP, WB

Protein disulfide isomerase (PDI) is an acidic 55 kDa thyroid hormone-binding protein that appears to be identical to the glial-p55 protein. It has been suggested to be involved in disulfide-bond formation and isomerization, as well as the reduction of disulfide bonds in proteins.

Glucose-regulated protein 94, also known as Grp94 or gp96, is an abundant resident endoplasmic reticulum (ER) lumenal stress protein.

Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum.

ERp29 resides in the lumen of the ER and is thought to play a role in the processing of secretory proteins within the ER.

ERp57 functions together with calnexin and calreticulin as a molecular chaperone of glycoprotein biosynthesis within the ER.

Within the ER, misfolded proteins are detected by Bip(GPR78).

HO-1 catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, iron and biliverdin that is immediately reduced to bilirubin.

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles.

WFS1 plays an important role in maintaining homeostasis of the endoplasmic reticulum (ER) in the pancreas.


Golgi Complex Markers

GlcNAc transferase AntibodyNB100-57845

Glucose-6-Phosphate AntibodyNB100-236


Detection of G6PD in HUVEC cellsusing NB100-236..

Species: Rt, MuApplications: ELISA, WB

Detection of GlcNAc in rat pancreas lysate using NB100-57845.

Syntaxin-6 AntibodyNB100-2920 TGN38 Antibody

NB110-40768

Species: Mu, RtApplications: IF, WB

Immuno-fluorescence staining of TGN38 in 3% paraformalde-hyde-fixed NRK cells.

P58 Antibody NB110-57306



Detection of Syntaxin-6 in human lymph node lysate using NB100-2920.

Detection of p58in HeLa cell lysate using NB110-57306.

VPS45 AntibodyNB100-2431

VPS45 AntibodyNB100-41372


Detection of VPS45 in human peripheral blood mononucleocyte lysate usingNB100-41372.

TGN38 Antibody NB110-60519

Species: Mu, RtApplications: IF, IHC-P, WB


Detection of VPS45 in human placenta lysate using NB100-2431.

Immunofluorescence staining of TGN38 in 3% paraformaldehyde-fixed NRK cells.

The Golgi apparatus is a membrane-bound stack of cisternae that processes proteins and lipids synthesized in the ER and packages them for transport elsewhere in the cell. The Golgi is responsible for glycosylating

proteins synthesized in the ER and also for cleaving some proteins into smaller fragments. The Golgi packages these products in vesicles for transport, either to destinations inside of the cell or outside of it.

Golgi Marker 58K AntibodyNB600-412

Species: Ca, Ha, Hu, RtApplications: ICC, IF, IHC, WB

Staining of HeLacells using NB600-412.

58K protein antibodies are excellent for use as markers for the Golgi complex.

GlcNAc transferase is present as an integral Golgi membrane protein and at least the catalytic center of the transferase is on the lumenal side of the vesicles.

Glucose-6-Phosphate is an indigenous component of Golgi membranes.

TGN46 AntibodyNB110-62093

Species: Hu, MkApplications: IF, WB

Immunofluorescence staining of human TGN46 in 3% paraformaldehyde-fixed HeLa cells.


Species: Hu, MkApplications: IF, WB

Detection of TGN46 in HeLa cell lysate using NB110-40769.


Species: Hu, MkApplications: IF, IHC-P, WB

Immunofluorescence staining of human TGN46 in 3% paraformaldehyde-fixed HeLa cells.

TGN46 is localized primarily in the Trans-Golgi network. TGN46 has been reported as being the best available marker for human trans-Golgi network.

P58 is a marker of pre-Golgi intermediate compartment (IC) and a putative marker for peripheral punctate pre-Golgi interme-diates and the cis-Golgi network.

Syntaxin-6 is primarily on the trans-Golgi network (TGN), where it partially colocalizes with the TGN adapter protein AP-1 on clathrin-coated membranes.

TGN38 is a a 38 kDa protein which has been shown to be predominantly localized to the trans Golgi network.

VPS45 is a member of the sec1 family implicated in Golgi to prevacuolar compartment trafficking in yeast. Mammalian VPS45 is found in multiple tissues, is partially membrane associated, and is enriched in the Golgi region.


Lysosome Markers

LAMP2 AntibodyNB110-11470

LIMPII/lgp85 AntibodyNB400-129

Species: Ca, Hu, Mu, Rt Applications: ICC, IHC, IP, WB

Staining of LIMP II in rat infarctzone tissue using NB 400-129 with a DAB detection system.

LAMP1 Antibody NB120-19294

Species: HuApplications: IF

Species: Ha, Hu, Mu, Mk, Rt, RbApplications: ICC, IF, IHC, IHC-P, IP, WB

Staining of mouse astrocytes using NB110-11470.

Rap1 AntibodyNB110-57453

Rap1 AntibodyNB110-57454

Species: Hu, Mu, RtApplications: FACS, ICC, IHC, IP, WB

Staining of paraffin-embedded human breast cancer tissue using NB110-57454.

Rap1 Antibody NB100-292

Species: HuApplications: IF, IP, WB


Staining of paraffin-embedded human stomach carcinoma using NB110-57453.

Localization of RAP1 to Telomeres with TRF2 by Immunofluorescence, using NB 100-292. Sample: HeLa cells.

Immunofluorescence staining of Lamp1 (red) in HeLa cells using N120-19294.

Mitochondrial Markers

Cytochrome c Antibody NB300-1068

GFP AntibodyNB100-1770

Species: AllApplications: ELISA, WB

GFP recombinant protein detected with NB 100-1770 polyclonal anti-GFP antibody.

Cytochrome c Antibody NB100-56503

Species: Dr, Ha, Eq, Hu, Mu, RtApplications: ICC, IF, WB

Species: HuApplications: ELISA, IHC-P, WB

Staining of hearttissue usingNB300-1068.

GFP AntibodyNB600-597

Species: AllApplications: ELISA, IHC, WB

Western blot of GFP recombinant protein detected with NB600-597.

GFP Antibody NB600-303

Species: AllApplications: ICC, IHC-P, IP, WB

Recombinant GFP detected using NB600-303.

Detection of Cytochrome C in HeLa cell lysate using NB100-56503.

Lysosomes are membrane-bound vacuoles which play a critical role in cellular metabolism. These organelles participate in endocytosis, the synthesis and assembly of

acid hydrolases, act as sites for digestion of foreign materials and for specialized autolytic cellular processes.

The mitochondrion is a compartmentalized, dual-membraned organelle that is responsible for most energy production in the cell. Mitochondria contain

their own DNA and ribosomes. The primary function of mitochondria is to convert energy from glucose into ATP via oxidative phosphorylation via the Krebs Cycle.

Lamp1, also known as lysosome associated membrane protein 1, is a heavily glycosylated membrane protein of the lysosome.

Lysosome-associated membrane protein 2 (LAMP 2), also known as lgp2 or lgp110, is a type 1 integral membrane protein that is transport-ed from the trans-Golgi network to endosomes and then lysosomes.

LIMP II is ubiquitously expressed in most higher organisms and is a major lysosomal and endosomal membrane marker.

Rap1A and Rap1B proteins were localized to late endocytic compartments (late endosomes/lysosomes) in fibroblasts.

GFP, green fluorescent protein, is a marker for subcellular localization and as a label for plant mitochondria in vivo.

Cytochrome c is released from mitochondria after tissue injury. It is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP.


Glutamate dehydrogenase AntibodyNB600-853

GRK2 AntibodyNB300-156

Species: Hu, Bv, RtApplications: IHC, WB

NB300-156 (green) versus NF-M (red, NB 300-134), in rat cortex, showing mostly large pyramidal neurons.

Species: BvApplications: ELISA, WB

Detection of Glutamate dehydrogenasein bovine liver preparations using NB600-853.

HSP60 AntibodyNB110-57063

HSP70 Mitochondrial AntibodyNB300-527

Species: Hu, MuApplications: ICC, IP, WB

Staining of human peripheral blood mononucleocyte lysate using NB300-527.

GRK2 Antibody NB100-2831


Species: Hu, Mu, RtApplications: IHC, WB

Staining of paraffin-embedded human human breast carcinoma using NB110-57063.

Detection of GRK2 in Daudi cell lysateusing NB100-2831.

Nuclear Markers

Histone H1 AntibodyNB300-373

Fibrillarin AntibodyNB300-269


Human SH-SY5Y cells stained with NB300-269, showing prominent specular nucleolar stainingNB.

NUP214 Antibody NB110-40634

Species: HuApplications: ICC, IHC, IHC-P

Species: Bv, Ch, Hu, Mu, Rb, RtApplications: IHC, WB

Detection of HistoneH1 in calf thumus us-ing NB300-373.

Staining of human ovarian carcinoma usingNB110-40634.

The nucleus is the central organelle of the cell, containing a cell’s DNA and coordinating the actions of the cell and the other organelles within the cell. Surrounded by the nuclear envelope, the nucleus is responsible for the control of cell division and the

direction of protein synthesis within the cell. The nucleolous, a membraneless organelle lying within the nucleus, manufactures ribosomes for protein synthesis in the cell.

Glutamate dehydrogenase has long been used as a marker for mitochondria in brain and other tissues.

HSP60 is a transiently expressed mitochondrial protein.

HSP70 is known to have been acquired from the proto-mitochondrial endosymbiont.

GRK2 is found in many cell types and may also have a role in the regulation of the microtubular cytoskeleton. The levels of GRK2 enzymatic activity, protein and mRNA are all elevated in a variety of human heart disease states, and the effect of this may contribute to the reduced effectiveness of the beta-adrenergic system in these disease states. GRK2 is also overexpressed in Alzheimer disease and is an early marker of brain mitochon-drial lesions.

Mitofilin AntibodyNB100-1919

Species: Ca, Hu, Mu, Rt, FeApplications: IF, IP, WB

Staining of HeLa cells using NB100-1919..

Mitochondrial Marker AntibodyNB120-17857

VDAC1 AntibodyNB100-695

Species: Hu, RtApplications: ELISA, WB

Detection of VDAC1in rat heart lysateusing NB100-695.


Staining of human liver using NB120-17857.

Analysis of the human heart mitochondrial proteome shows that mitofilin is one of the most abundant mitochondrial proteins.

NB120-17857 recognizes a 60kDa non-glycosylated protein component of mitochondria found in human cells. It can be used to stain the mitochondria in human samples as a mitochondrial-marker in subcellular fractions.

VDAC1 is an outer membrane mitochondrial protein. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest.

Nucleoporin 214 (NUP214) is required for CRM1-mediated nuclear protein export as well as cell cycle progression.

Somatic H1 may act as a marker of nuclear reprogramming.

Fibrillarin is an snRNP protein involved inpre-ribosomal RNA processing.


Cancer MarkersBreast CancerBreast cancer is the most common cancer in women and is second to lung cancer as the most common non-skin cancer overall. Approximately 200,000 cases of breast cancer are diagnosed in women every year in

the United States alone, with an additional 2,000 cases diagnosed in men. There will be an estimated 40,000 women and 450 men who die in 2008 from breast cancer in the United States.

Androgen Receptor Antibody NB120-9474

Species: HuApplications: IHC, WB

Staining of formalin fixed paraffin embed-ded human prostate using NB120-9474.

Androgen Receptor AntibodyNB300-615

Bcl-2 AntibodyNB200-572


Staining of formalin-fixed, paraffin-embedded human tonsil using NB200-572.

Species: Hu, Mu, Mk, RtApplications: ICC, IHC, IP, WB

Staining ofprimate brain using NB300-615.

Beta-Catenin AntibodyNB110-56924



CAIX AntibodyNB100-417

CaR AntibodyNB300-601

Species: Hu, RtApplications: ICC, IHC, IP, WB

Staining of Rat-1 cells using NB300-601.

Species: Ca, HuApplications: IF, IHC-P, WB

Staining of human RCC tumor cryosections using NB100-417.

CD24 AntibodyNB110-500


Staining of formalin-fixed, paraffin-embedded human liver using NB110-500.

Cyclin A AntibodyNB110-56899



Staining of formalin fixed paraffin embedded human tonsil using NB110-14736.

Species: HuApplications: ICC, IHC, IP, WB

Staining of paraffin-embedded skin carcinoma using NB110-56899.




Cyclin E AntibodyNB110-56904

Cyclin E AntibodyNB110-56905



Species: HuApplications: FACS, ICC, IP, WB


DAP-kinase AntibodyNB110-56925



DAP-kinase AntibodyNB110-56926

ErbB2 AntibodyNB100-1739


Staining of human breast adenocarcinoma usingNB100-1739.

Species: Hu, Mu, RtApplications: FACS, IHC, WB

Immunohistochemical staining of paraffin-embedded human liver carcinoma using NB110-56926.


ErbB-2 Antibody NB110-57022

Species: HuApplications: FACS, IHC, IP, WB

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using NB110-57022.

ErbB-2 AntibodyNB110-55529



Immunohistochemical staining of paraffin-embedded Human breast carcinoma using NB110-57021.




Species: HuApplications: FACS, ICC, IHC, IP

Immunohistochemical staining of paraffin-embedded human breast ductal carcinoma in situ using NB110-57023.

EZH2 AntibodyNB110-56975

GATA3 AntibodyNB600-1184


IHC staining of paraffin embedded human tonsil using NB600-1184.

Species: Hu, Mu, RtApplications: FACS, ICC, IF, IP, WB


GCDFP-15 AntibodyNB110-66658


Immunohistochemical staining of paraffin-embedded human breast carcinoma using NB110-66658.

Glut-1 AntibodyNB120-15310

HIF-1 alpha AntibodyNB100-479

Species: Hu, Mu, Mk, RtApplications: IHC, WB

Staining of placenta, villi using NB100-479.

Species: Hu, RtApplications: IHC-P

Staining of human esophagous usingNB120-15310.

HSP70 AntibodyNB110-57064

Species: HuApplications: FACS, IHC, IP, WB

Immunohistochemical staining of paraffin-embedded human prostate carcinoma using NB110-57064.

Ki67 AntibodyNB500-170

KPNA2 AntibodyNB100-1371

Species: HuApplications: ELISA, ICC, IHC, WB

Staining of greenformaldahyde-fixed HeLa with endogenously expressed Histone H2B-GFP fusion as DNA marker.

Species: Hu, Mu, Po, RtApplications: ICC, IF, IHC-Fr, IHC-P

Staining of FFPE human tonsil using NB500-170.



Staining of rat spinal cord and dorsal root paraformaldehyde/paraffin-embedded tissue usingNB300-144.

MDM2 AntibodyNB100-62269

MMP-1 AntibodyNB110-57211

Species: HuApplications: FACS, ICC, IHC, IP, WB

Immunohistochemical staining of paraffin-embedded human cervical carcinoma using NB100-57211.

Species: HuApplications: IHC-Fr, IHC-P, IP, RIA, WB

Staining of MDM2- human breast carcinoma using NB100-62269.



Staining of formalin fixed and Paraffin embedded human placenta tissue using NB110-60992.


p53 AntibodyNB100-1746


Staining of human skin squamous cell carcinoma using NB100-1746.

Species: Ch, Hu, Mu, RtApplications: ELISA, IHC-Fr, IHC-P, WB

Detection of MMP2 in a paraffin-embedded human neurofibroma using NB200-193.


p63 Antibody NB100-691

Species: Hu, Mu, RtApplications: IF, IHC-P, IP, WB

Staining of formalin fixed paraffin embedded human skin using NB100-691.

PKC alpha AntibodyNB110-57353

PSA AntibodyNB600-835

Species: HuApplications: IHC-P, WB

Staining of formalin-fixed, paraffin-embedded human prostate carcinoma using NB600-835.


Staining of human thyroid gland carcinoma using NB110-57353.

SNCG AntibodyNB110-60497

Species: HuApplications: ELISA, IHC-Fr, IHC-P, WB

Immunohistochemical staining of paraffin-embedded human ovary carcinoma usingNB110-60497.


Species: Hu, Mu, RtApplications: ICC, IF, IHC, IHC-P, WB

Staining of ovarycarcinoma usingNB500-238.

Colorectal CancerAlthough only a quarter of diagnosed colorectal tumors are malignant, this portion still accounts for 150,000 cases of colorectal cancer in the United states each year, resulting in nearly 50,000 deaths. Nearly all

malignant colorectal tumors are adenocarcinomas, although lymphomas, leiomyosarcomas, and carcinoids can also occur in the colorectal region.

Bcl-2 AntibodyNB200-572


Formalin-fixed, paraffin-embedded human tonsil stained using NB 200-572.

Caspase 7 AntibodyNB110-55664

CDX-2 AntibodyNB110-40734


Staining of FFPE human ovarian carcinoma using NB110-40734.



CDX-2 AntibodyNB100-699


Immunohistochemical staining using NB100-699.

Cyclooxygenase-2 AntibodyNB100-689

Galectin-3 AntibodyNB300-538

Species: Hu, Mu, RtApplications: ELISA, FACS, IF, IHC, IP, WB

Staining of rat olfactory bulb using NB300-538.

Species: Hu, Mu, RtApplications: IHC-Fr, IHC-P, IP, WB

Staining of formalin fixed paraffin embedded human colon carcinoma using NB100-689.


Species: Hu, Mu, Po, RtApplications: ICC, IF, IHC-Fr, IHC-P

Staining of FFPE human tonsilusing NB500-170.




Immunohistochemical staining of paraffin-embedded human lymphoma using NB110-57222.

Species: HuApplications: IHC, IHC-Fr, WB

Staining of human lymph node usingNB600-1209.


Species: Hu, Mu, Ca, Rt, FeApplications: IF, WB, IHC-P, IP, ICC.

Telophase with accumulation of survivin in the mid-bodies of two daughter cells.

For research purposes only.Not for use in humans.Prices subject to change. FAX: 303.730.1966 • WEB: www.novusbio.com

MMP-9 Antibody NB110-57223

Species: HuApplications: FACS, IHC, WB



MUC1 AntibodyNB120-15481

Species: Hu, MuApplications: IHC-P, WB



Staining of skingranulation tissue usingNB100-59029.

Osteopontin AntibodyNB600-1043

Species: Ca, Hu, PoApplications: IHC, WB

Staining of humanbreast tumor cellsusing NB600-1043.



Species: Hu, RtApplications: ELISA, IHC-P, WB

Staining of rat abdominal tissue using NB600-1160.



SMAD4 AntibodyNB110-57582


Immunohistochemical staining of paraffin-embedded human lung carcinoma using NB110-57582.

STAT3 AntibodyNB110-57596

Telomerase AntibodyNB600-476

Species: HuApplications: IF, IHC, IP, WB

Immunofluorescence microscopy of Saos-2 cells transduced with a retroviral vector expressing hTERT and GFP rom an internal ribosomal entry site.


Immunohistochemical staining of paraffin-embedded lung squamous carcinoma using NB110-57596.

TEM-8 AntibodyNB110-17405

Species: Hu, Mu, RtApplications: IHC, WB

Immunohistochemi-cal staining of human brain tissue using NB110-17405.

VEGF-D AntibodyNB110-60973


Formalin fixed and Paraffin embedded human placenta tissue using NB110-60973.

MUC16 AntibodyNB200-562

Species: HuApplications: ELISA, IHC-P, WB

Staining ofhuman lung tissue (alveolocytes type II) usingNB200-562.

Calcitonin AntibodyNB110-55646

CEA AntibodyNB120-15987

Species: HuApplications: ELISA, IHC-P

Staining of formalin fixed paraffin embedded human colon carcinoma using NB120-15987.


Immunohistochemical staining of paraffin-embedded human thyroid usingNB110-55646.

Lung CancerLung cancer is the most common non-skin cancer, with an estimated 215,000 new cases diagnosed in 2008 in the United States. Additionally, more than 160,000 people will die in the United States from lung cancer in 2008, making lung cancer the leading cause of cancer

deaths. While most cases of lung cancer are related to smoking, there are other risk factors including genetics, family history of lung cancer and exposure to chemi-cals and pollution.


Species: HuApplications: FACS, IF, WB

Staining of normalpancreas using NB100-317.


Species: HuApplications: IF, IHC, IP, WB

COX-2 AntibodyNB100-689

Species: Hu, Mu, RtApplications: IHC-Fr, IHC-P, IP, WB

Staining of formalin fixed paraffin embed-ded human colon carcinoma usingNB100-689.

CEA AntibodyNB120-15287


Staining of formalin fixed paraffin embedded human breast using NB120-15287.

hTERT AntibodyNB100-317

Species: HuApplications: FACS, IHC-P, IF, WB

Staining of pancreatic carcinoma usingNB100-317.



Species: Bv, Hu, Mu, RtApplications: IF, WB

Rat neurons stained with anti-MAP2 (NB 300-213) [green] and anti-GFAP (NB 300-142) [red].


Immunohistochemical staining of paraffin-embedded Human tonsil usingNB110-57147.

NSE AntibodyNB110-58870


Staining of HEL293 cells using NB110-58870.

NSE AntibodyNB100-1606


Species: HuApplications: ELISA, IHC, IHC-P, WB

Staining of paraffin embedded human tonsil usingNB100-1883.


Dissociated cell cultures of neonatal mouse brains, showing NSE-2 (green staining) in neurons.



Staining of A431 cells using NB110-57309.


Smac AntibodyNB110-56936

Species: Hu, RtApplications: FACS, ICC, IHC, IP, WB

Immunohistochemical staining of paraffin-embedded human skin cancer tissue using NB110-56936.


Staining of humanbrain using NB300-675.


Species: HuApplications: IF, IHC, WB

Staining of lungcarcinoma usingNB500-237.


Immunofluorescence staining of Saos-2 cells transduced with a retroviral vector expressing hTERT using NB600-476.


UCHL1 AntibodyNB300-676

Staining of humanbrain using NB300-676.


Species: Hu, Mu, RtApplications: IF, IHC-P, IP, WB

Staining of formalin fixed paraffin embedded human skin using NB100-691.


Species: Ca, Hu, PoApplications: IHC, WB

Staining of human breast tumorusing NB600-1043.


Species: MuApplications: ICC, IHC, IHC-P, WB

MAP-2-positive cultured neurons (green) from an e13.5 mouse brain. Nuclei are blue.


Species: Hu, RtApplications:IHC-Fr, IHC-P

Staining of formalin-fixed, paraffin-embedded human tonsil using NB600-1252.


Prostate CancerFor men in the United States, prostate cancer is the most common non-skin cancer and is the second most deadly cancer. With over $8 billion spent annually in the United States on prostate cancer research, prostate

cancer diagnoses and deaths have declined in recent years after rising in the 1980s because of new detection techniques. Nearly 1 in 6 men will be diagnosed with prostate cancer at some point in their lives.

Bcl-2 Antibody NB110-55551

Species: Hu, MuApplications: FACS, ICC, IHC, IP, WB


Caveolin-1 AntibodyNB100-615



Staining of respiratory epithelium and alveole using NB110-39015.

Species: Hu, Mu, RtApplications: IF, IP, WB

Staining of EaHy926 endothelial cell line using NB100-615.



Staining of respiratory epithelium, bronchial smooth muscle, and alveoli using NB110-39016.

DPP4 AntibodyNB100-58756

DPP4 AntibodyNB100-59021


Staining of placenta using NB100-59021.


Staining of placenta using NB100-58756.

E-cadherin AntibodyNB110-56937


Immunohistochemical staining of paraffin-embedded human hepatocellular carci-noma using NB110-56937.


HGF AntibodyNB110-57200

Species: HuApplications: ICC, IHC, WB




Species: HuApplications: IHC, IHC-Fr, WB

Staining of human lymph node usingNB600-1209.

LDH AntibodyNB110-57161

MCM7 AntibodyNB110-57188


Immunohistochemical staining of paraffin-embedded human lung tissue using NB110-57188.

Species: HuApplications: FACS, IHC, WB

Immunohistochemical staining of paraffin-embedded human testis carcinoma using NB110-57161.

PCNA AntibodyNB500-106

Species: Ch, Dr, Hu, Mu, Rt, YeApplications: IHC, IP, WB

Immunohistochemical staining of paraffin-embedded human colon carcinoma, using NB500-106.


S100A6 AntibodyNB100-1765

Species: HuApplications: ELISA, IHC, WB

Immunohistochemical staining of paraffin-em-bedded brain glioma tissue using NB100-1765.


Staining of formalin-fixed, paraffin-embedded human prostate carcinoma using NB600-835.

Immmunohistochemical staining of paraffin-embedded human prostate carcinoma using NB110-59960.


OR

DE

RIN

GU.S. ORDERING

Phone: 303.730.1950

888.506.6887

Fax: 303.730.1966

Email: [email protected]

Web: www.novusbio.com

EUROPEAN ORDERINGNovus Europe Phone: +44 (0) 1223 426001Fax: +44 (0) 871 971 1635Email: [email protected]: www.novusbio.com Novus Germany Phone: +49-6922-22340-60Fax: +49-0800-58926-79Email: [email protected]: www.novusbio.com

CANADIAN ORDERINGPhone: 905.827.6400Phone: 855.668.8722Fax: 905.827.6402Email: [email protected]: www.novusbio.com

TECHNICAL SUPPORTPhone: 303.730.1950 888.506.6887Email: [email protected]

Chat with a Scientist featured on our website: www.novusbio.com

Novus Italy Phone: +39 02 4032 6786 Fax: +39 02 4032 6340Email: [email protected]: www.novusbio.com

CATALOG OF ANTIBODIES FOR MARKERS - Novus Biologicalsimages.novusbio.com/design/markers.pdf ·...

Documents

Transcript of CATALOG OF ANTIBODIES FOR MARKERS - Novus Biologicalsimages.novusbio.com/design/markers.pdf ·...