Cat. #RR039A Premix Ex Taq™ (Perfect Real Time) … · Table of contents I. Description ......

Table of contents

I. Description..................................................................................................................2

II. Principle........................................................................................................................2

III. Content.........................................................................................................................3

IV. Storage..........................................................................................................................4

V. Features........................................................................................................................4

VI. PrecautionsforUse..................................................................................................4

VII. Protocol........................................................................................................................4

A)ProtocolusingSmartCycler®IISystem...................................................4

B)ProtocolusingABIPRISM®7000/7700/7900HT＆

7300/7500/7500FastReal-TimePCRSystem.......................................7

C)ProtocolusingLightCycler®.........................................................................9

D)ProtocolusingMx3000P............................................................................ 12

VIII. ApplicationExample............................................................................................ 14

IX. Relatedproducts.................................................................................................... 14

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Premix Ex Taq™ (Perfect Real Time) Cat.#RR039Av1103Da

URL:http://www.takara-bio.com

I. DescriptionPremixExTaq™(PerfectRealTime)isa2×convenientpremixreagent,speciallydesignedforrealtimePCRbyusingTaqMan®＊ 1probe.ThisproductcombinesthehighperformanceofTaKaRaExTaq™HS,whichisanenzymeforhotstartPCRutilizingTaqantibody,withanewlydevelopedbufferwhichprovidessuperiorspecificity,increasedamplificationefficiencyandhighaptitudeforhigh-speedrealtimePCR.Accordingly,asuccessfulrealtimePCRisprom-isedwithhighsensitivity,widedynamicrangeandaccuratequantification.

ApplicablerealtimePCRinstruments；･ ABIPRISM®7000,7700,7900HT,＊2 AppliedBiosystems7300,7500Real-TimePCRSystem, 7500FastReal-TimePCRSystem(LifeTechnologies)･ LightCycler®(RocheDiagnostics)＊3･ SmartCycler®System/SmartCycler®IISystem(Cepheid)＊4･ Mx3000P(Stratagene)･ ThermalCyslerDice™RealTimeSystemII (TaKaRaBio)＊5

＊ 1:TaqMan®isatrademarkofRocheMolecularSystemsInc.＊ 2:ABIPRISM®isaregisteredtrademarkofAppleraCorporation.＊ 3:LightCycler®isaregisteredtrademarkofRocheDiegnostics.＊ 4:SmartCycler®isatrademarkofCepheid.＊ 5:ThisinstrumentisnotavailableintheU.S.andEurope.

II. PrincipleThisproductemploysTaKaRaExTaq™HSforPCRreaction.PCRproductsaredetectedwithTaqMan®probeinrealtimemonitoring.

1)PCRPCR(PolymeraseChainReaction)isasimpleandpowerfulmethodtoamplifyonlyatargetDNAofatinyamountbycyclingthreeincubationstepsatdifferenttemperatures:Double-strandedtargetDNAisheatdenatured(denaturationstep),thetwoprimerscomplementarytothetargetsegmentareannealedatlowtemperature(annealingstep),andtheannealedprimersarethenextendedatanintermediatetemperature(extensionstep)withaDNApolymerase.Asthetargetcopynumberdoublesuponeachcycle,PCRcantherebyamplifyDNAfragmentsupto106-foldinashortperiod.AsthisproductutilizesanenzymeforHotStartPCR,TaKaRaExTaq™HS,non-specificam-plificationduetomisprimingpriortocyclingorduetoprimerdimmercanbeminimized.Accordinglythehighlyspecificandsensitivedetectionisachieved.

2)Fluorescencedetection:TaqMan®Probemethod:TheTaqMan®methodisbasedonacombinationofTaqMan®TechnologyandarealtimePCRinstrument.Oligonucleotidemodifiedwithfluorescencesubstance(e.g.FAM)at5'-endandwithquencher(e.g.TAMRA)at3'-endisaddedinareactionsystem.Undertheannealingcondition,TaqMan®probespecificallyhybridizestotemplateDNA,buttheflu-orescenceissuppressedbyquencher.Inextensionstep,the5'→3'exonucleaseactivityofTaqDNApolymerasedegradestheTaqMan®probehybridizedtoatemplate.Accordingly,TaqMan®probeisreleasedfromthesuppressionwithaquencher,resultinginemissionoffluorescence.Bymeasuringthefluorescenceintensitytheamountofamplifiedproductcanbemonitored.

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III. Content (250 units)PremixExTaq™(PerfectRealTime)(2×conc.)＊1 1.0ml×5tubesROXReferenceDye(50×conc.)＊2 200μlROXReferenceDyeII(50×conc.)＊2 200μl

＊ 1:containsTaKaRaExTaq™HS,dNTPMixture,andMg2+.＊ 2:ROX™ReferenceDye/DyeIIisusedfornormalizationofintensitybybackgroundsubtrac-ROX™ReferenceDye/DyeIIisusedfornormalizationofintensitybybackgroundsubtrac-

tion.ForABIPRISM®7000/7700/7900HTandAppliedBiosystems7300Real-TimePCRSystem,theuseofROX™ReferenceDye(50×)isrecommended.ForAppliedBiosystems7500Real-TimePCRSystem,7500FastReal-TimePCRSystem,andStratageneMx3000P,theuseofROX™ReferenceDyeIIisrecommended.

ItisnotrequiredforusewithSmartCycler®,LightCycler®orThermalCyclerDice™RealTimeSystemII .

Reagents and Instruments Required but Not Supplied in this product

1.ThermalCyclerforrealtimePCR(authorizedinstruments)2.ReactiontubeorplateforrealtimePCR3.PCRprimers4.TaqMan®probefordetection(licensedprobes)5.dH2O6.MicropippetsandMicropippettips(autoclavedpriortouse)

QF

Q

Q

F

F

F Q

2)Primerannealing/Probehybridization

Polymerase

Primer

ProbeFluorescenceSubstance

Quencher

Hybridizes

1)Heatdenaturation

3)Extension

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IV. StorageStableat4℃for6months.Carefulattentionshouldbemadenottocausecontamination.

＊Thisproductisshippedat-20℃.Afterdelivered,storeat4℃.Maketheconcentra-Thisproductisshippedat-20℃.Afterdelivered,storeat4℃.Maketheconcentra--20℃.Afterdelivered,storeat4℃.Maketheconcentra-20℃.Afterdelivered,storeat4℃.Maketheconcentra-tionuniformbygentlyinvertingatubebeforeuse.

＊Thisproductcanbestoredat-20℃forlongtermstorage.Oncethawed,storeat4℃anduseupwithin6months.

V. Features1) Quick＆accuratedetectionandquantificationoftargetgenethroughrealtimePCR.2) Easy-to-use2×premixreagentdesignedforreducingpipettingsteps.3) Highamplificationefficiencyandhighlysensitivedetection:Thisproductutilizesanenzymeforhotstart,TaKaRaExTaq™HS.Asthisenzyme-buffersystemisoptimizedforrealtimePCR,thisproductoffershighlyefficientamplificationandhighsensitivedetection.

VI. Precautions for UsePleaseensuretoreadthroughthefollowingprecautionspriortostartingtheprotocol.

1) Priortouse,maketheconcentrationuniformbygentlyinvertingatubebeforeuse.2) Aprecipiatemayforminthisproductduringstorageat-20℃.Thisprecipitatecanbecompletelydissolvedbybrieflywarmingthetubewithyourhandsorplacingthetubeatroomtemperature,followedbyinversionofthetubeseveraltimestomixuntildissolved.USETHEREAGENTONLYAFTERCOMPLETELYREDISSOLVINGTHEPRECIPITATEtoensureauniformconcentrationofcomponents.Donotvortex.Evenintheabsenceofaprecipitate,gentlemixingoftheproduct(avoidingairbubbles)isrecommendedtoprovideauniformconcentrationofcomponentspriortouse.

3) TaqMan®probefordetectionshouldbepreparedseparately.Itisnotsuppliedinthisproduct.

4) Forpreparinganddispensingthereagents,anewdisposabletipshouldbeusedtominimizecontaminationamongsamples.

VII. ProtocolA) Protocol using Smart Cycler® II System1.PreparethefollowingPCRreactionmixture.<perreaction>[Reagents] [Volume] [Finalconcentration]PremixExTaq™(2×) 12.5μl 1×PCRForwardPrimer(10μM) 0.5μl 0.2μM＊1PCRReversePrimer(10μM) 0.5μl 0.2μM＊1TaqMan®probe 1μl ＊2

template 2μl ＊3

dH2O 8.5μltotal 25μl

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＊ 1:Thefinalconcentrationofprimerscanbe0.2μMinmostreactions.Whenitdoesnotwork,determinetheoptimalconcentrationswithintherange0.1-1.0μM.

＊ 2:ProbeconcentrationdiffersdependingonkindofrealtimePCRinstrument,andkindoffluorescencelabelingmaterial.Theaddingamountshouldbede-terminedbyreferringtotheoperationmanualofinstrumentandaproductinsertsuppliedwithprobe.GenerallywhenperformingdetectionwithSmartCycler®System,probeconcentrationshouldbedeterminedtohavethefinalconcentrationwithintherange0.1-0.5μM.

＊ 3:Finaltemplateconcentrationvariesdependingonthecopynumberoftargetpresentinthetemplatesolution.Optimalamountshouldbede-terminedbypreparingthedilutionseries.DNAtemplateshouldbeusedinlessthan100ng.WhentheRTreactant(cDNA)isusedasatemplate,itshouldbeaddedinless10%volumeofPCRreactionmixture.

2.Startthereaction.GentlycentrifugethereactiontubesusingthecentrifugeexclusiveuseforSmartCycler®.LoadthereactiontubesonSmartCycler®IISystemandstartthereaction.ShuttlePCRstandardprotocol(below)isrecommended.Trythisprotocolfirstly,andoptimizethereactionconditionifneeded.

ShuttlePCRStandardProtocolStage1:InitialDenaturation Stage2:PCRreaction

Note : ThisproductcombinesthehighperformanceofTaKaRaExTaq™HS,whichisanenzymeforhotstartPCRutilizingTaqantibody.InitialdenaturationsteppriortoPCRshouldbeat95℃for30sec.Noneedtoheatat95℃for(5-)15min.astheinitialdenaturation,requiredforchemicallymodifiedTaqpolymerase.Ifexcessiveheattreatmentisprovided,theenzymeactivitydescreasesandtheamplificationef-ficiencyandtheaccuracyinquantificationcanalsobeaffected.

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InitialDenaturationStep Temperature Time Detection Remark

InitialDenaturation 95℃ 30sec. OFF

Itisrecommendedtoperforminitialdenaturationat95℃for30seconds,whichisenoughevenwhenthetemplateisgenomicDNAorcircularplasmid.Dena-turationconditionthatexceeds2minutesunstabilizesthereactionthusnotrecommended.

ShuttlePCRStep Temperature Time Detection Remark

Denaturation 95℃ 3-5sec. OFF

GeneralamplifiedsizeforrealtimePCRislessthan300bp,sodena-turationat95℃for3-5secondswouldbesufficient.

Annealing/Extension 56-64℃ 20-30sec. ON

Pleasetryat60℃for20secondsatfirst.Thetemperatureshouldbeoptimizedwithintherangeof56-64℃ifoptimizationisrequired.Whenreactiondoesnotproceedefficiently,extendthetime.

Cyclenumber;30-45cycles

3.Afterthereactioniscompleted,performanalysis.Afterthereactioniscompleted,verifytheamplificationcurve.Establishthestandardcurvewhenquantificationisdone.

＊ RefertotheoperationmanualofSmartCycler®fortheanalysismethodwithSmartCycler®.

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B) Protocol using ABI PRISM 7000/7700/7900HT or Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System

1.PreparePCRreaction(below).

<perreaction>[Reagents] [Volume] [Volume] [Finalconc.]PremixExTaq™(2×) 10μl 25μl 1×PCRForwardPrimer(10μM) 0.4μl 1μl 0.2μM＊ 1PCRReversePrimer(10μM) 0.4μl 1μl 0.2μM＊ 1TaqMan®probe 0.8μl 2μl ＊ 2

ROXReferenceDyeorDyeII(50×) ＊ 3 0.4μl 1μl 1×template 2μl 4μl ＊ 4

dH2O 6μl 16μltotal 20μl ＊ 5 50μl ＊ 5


＊ 2:ProbeconcentrationdiffersdependingonkindofrealtimePCRinstrument,andkindoffluorescencelabelingmaterial.Theaddingamountshouldbedeter-minedbyreferringtotheoperationmanualofinstrumentandaproductinsertsuppliedwithprobe.

＊ 3:TheROX™ReferenceDye/DyeIIissuppliedforperformingnormalizationoffluo-3:TheROX™ReferenceDye/DyeIIissuppliedforperformingnormalizationoffluo-rescentsignalintensitiesamongwellswhenusedwithrealtimePCRinstrumentsthathaveoption.ForABIPRISM®7000/7700/7900HTandAppliedBiosystems7300Real-TimePCRSyatem,theuseofROX™ReferenceDye(50×)isrecom-mended.ForAppliedBiosystems7500Real-TimePCRSystemand7500FastReal-TimePCRSystem,theuseofROX™ReferenceDyeIIisrecommended.

＊ 4:Finaltemplateconcentrationvariesdependingonthecopynumberoftargetpresentinthetemplatesolution.Optimalamountshouldbedeterminedbypreparingthedilutionseries.DNAtemplateshouldbeusedinlessthan100ngfor20μlreaction.WhentheRTreactant(cDNA)isusedasatemplate,itshouldbeaddedinless10%volumeofPCRreactionmixture.

＊ 5:Thereactionof50μlisfor96-wellplate,singletubeand8-striptube.Thereactionof20μlisfor384-wellplateand96-wellFastThemalCyclingPlate.

2.Startthereaction.ShuttlePCRstandardprotocol(below)isrecommended.Trythisprotocolfirstly,andoptimizethereactionconditionifneeded.

Note : ThisproductcombinesthehighperformanceofTaKaRaExTaq™HS,whichisanenzymeforhotstartPCRutilizingTaqantibody.Initialdena-turationsteppriortoPCRshouldbeat95℃for30sec.Noneedtoheatat95℃for(5-)15min.astheinitialdenaturation,requiredforchemi--)15min.astheinitialdenaturation,requiredforchemi-)15min.astheinitialdenaturation,requiredforchemi-callymodifiedTaqpolymerase.Ifexcessiveheattreatmentisprovided,theenzymeactivitydescreasesandtheamplificationefficiencyandtheaccuracyinquantificationcanalsobeaffected.

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ShuttlePCRStandardProtocol

Stage1:InitialdenaturationReps:195℃30sec.

Stage2:PCRReps:4095℃5sec.60℃30sec.(31sec,34sec) ＊

＊: Fluorescencedetectionstepshouldbe30secondswithABIPRISM7700and7900HT,31secondswithABIPRISM7000and7300Real-TimePCRSystem,and34secondswith7500Real-TimePCRSystem.


HoldingStageReps：195℃,30min.

CyclingStageNumberofCycles:4095℃,3min.60℃,30min.

MeltCurveStage

[7500FastReal-TimePCRSystem]

[ABIPRISM7000/7700/7900HT,7300/7500Real-TimePCRSystem]

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InitialdenaturationstepStep Temperature Time Detection Remark

Initialdenaturation 95℃ 30sec. OFF




SincethetargetsizeamplifiedforrealtimePCRisgenerallyshorterthan300bp,thedenaturationat95℃for3-5secondsisasuf--5secondsisasuf-5secondsisasuf-ficientcondition.

Annealing/Extension 56-64℃

25-34sec.(25, 30, 31,34sec.) ＊

ON

Pleasetryat60℃for30,31,34or25secondsatfirst.Thetemperatureshouldbeoptimizedwithintherangeof56-64℃ifoptimizationisrequired.Whenreactiondoesnotproceedefficiently,extendthetime.

＊: Fluorescencedetectionstepshouldbe30secondswithABIPRISM7700and7900HT,31secondswith7000and7300,and34secondswith7500Real-TimePCRSystem.Whenusedwith7500Fast,set25sec.

Cyclenumber；30-45cycles

3. Afterthereactioniscompleted,verifytheamplificationcurve.Establishthestandardcurvewhenquantificationisdone.＊ RefertotheoperationmanualofanusedrealtimePCRinstrument.

C) Protocol using LightCycler®

1.PreparethefollowingPCRreactionmixture.<perreaction>[Reagents] [Volume] [Finalconcentration]PremixExTaq™(2×) 10μl 1×PCRForwardPrimer(10μM) 0.4μl 0.2μM＊1PCRReversePrimer(10μM) 0.4μl 0.2μM＊1TaqMan®probe 0.8μl ＊2

template 2μl ＊3


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＊ 2:ProbeconcentrationdiffersdependingonkindofrealtimePCRinstrument,andkindoffluorescencelabelingmaterial.Theaddingamountshouldbedeter-minedbyreferringtotheoperationmanualofinstrumentandaproductinsertsuppliedwithprobe.

＊ 3:Finaltemplateconcentrationvariesdependingonthecopynumberoftargetpresentinthetemplatesolution.Optimalamountshouldbedeterminedbypreparingthedilutionseries.DNAtemplateshouldbeusedinlessthan100ng.WhentheRTreactant(cDNA)isusedasatemplate,itshouldbeaddedinless10%volumeofPCRreactionmixture.

2.Startthereaction.AftergentlycentrifugationofLightCycler®capillaries,setcapillariesinaLightCyclerandstartthereaction.ShuttlePCRstandardprotocol(below)isrecommended.Trythisprotocolfirstly,andoptimizethereactionconditionifneeded.



Stage1:InitialdenaturationReps:195℃,30sec.20℃/sec.

Stage2:PCRReps:4095℃,5sec.20℃/sec.60℃,20sec.20℃/sec.

Stage2:PCRreaction

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Initialdenaturation

Step Temperature Time AcquisitionMode Remark

Initialdenaturation 95℃ 30sec. None


ShuttlePCR

Step Temperature Time AcquisitionMode Remark

Denaturation 95℃ 3-5sec. None


Annealing/Extension 56-64℃ 20-30sec. Single


Cyclenumber:30-45cycles

3. Afterthereactioniscompleted,verifytheamplificationcurve.Establishthestandardcurvewhenquantificationisdone.＊ RefertotheoperationmanualofanusedrealtimePCRinstrument.

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D) Protocol using Mx3000P

1.PreparethefollowingPCRreactionmixtureonice.

<perreaction>[Reagents] [Volume] [Finalconc.]PremixExTaq™(2×) 12.5μl 1×PCRForwardPrimer(10μM) 0.5μl 0.2μM＊1PCRReversePrimer(10μM) 0.5μl 0.2μM＊1TaqMan®probe 1μl ＊2

ROXReferenceDyeII(50×)＊3 0.5μl 1×template(<100ng) 2μl ＊4


＊ 1:Thefinalconcentrationofprimerscanbe0.2μMinmostreactions.Whenitdoesnotwork,determinetheoptimalconcentrationswithintherangeof0.1-1.0μM.

＊ 2:ProbeconcentrationdiffersdependingonkindofrealtimePCRinstrumentandtypeoffluorescencelabelingmaterial.Theaddingamountshouldbedeter-minedbyreferringtotheoperationmanualofinstrumentandaproductinsertsuppliedwithprobe.

＊ 3:ForMx3000P,theuseofROXReferenceDyeIIisrecomended.ROXReferenceDyeisnotsuitablefortheusewithMx3000P,sinceithashigherconcentrationthanROXReferenceDyeII.

＊ 4:Finaltemplateconcentrationvariesdependingonthecopynumberoftargetpresentinthetemplatesolution.Optimalamountshouldbedeterminedbypre-paringthedilutionseries.ItisrecomendedtoapplyDNAtemplateinlessthan100ng.WhentheRTreactant(cDNA)isusedasatemplate,itshouldbeaddedinlessthan10%volumeofPCRreactionmixture.

2.Startthereaction.ShuttlePCRstandardprotocol(below)isrecommended.Trythisprotocolfirstly,andoptimizethereactionconditionifneeded.

ShuttlePCRStandardProtocolStage1:Initialdenaturation Reps:1 95℃,30sec.

Stage2:PCR Reps:40 95℃,5sec. 60℃,20sec.


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InitialdenaturationStep Temperature Time Detection Remark

Initialdenaturation 95℃ 30sec. OFF





Annealing/Extension 56-64℃ 20-30sec. ON


Cyclenumber:30-45cycles

3.Afterthereactioniscompleted,performanalysis.Afterthereactioniscompleted,verifytheamplificationcurve.Establishthestandardcurvewhenquatitationisdone.＊ RefertotheoperationmanualofanusedrealtimePCRinstrument.

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95℃ 30sec.↓95℃ 3sec. 45cycle60℃ 25sec.

Amplificationcurve

R2=0.9998Slope=-3.417

Standardcurve

VIII. Application exampleDetection example with Applied Biosystems 7500 Fast Real-Time PCR System

Target:MouseGapdPrimer/Probe:TaqMan®GeneExpressionAssays(LifeTechnologies)Template:cDNAserialdilution(correspondingtototalRNA500fg-50ng)

IX. Related productsPrimeScript™RTreagentKit(PerfectRealTime)(Cat.#RR037A)OneStepPrimeScript™RT-PCRKit(PerfectRealTime)(Cat.#RR064A)SYBR®PremixExTaq ™II(TliRNaseHPlus)(Cat.#RR820A/B)SYBR®PremixExTaq ™(TliRNaseHPlus)(Cat.#RR420A/B)OneStepSYBR®PrimeScript™RT-PCRKitII(PerfectRealTime)(Cat.#RR086A)OneStepSYBR®PrimeScript™RT-PCRKit(PerfectRealTime)(Cat.#RR066A)

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NOTICETOPURCHASER:LIMITEDLICENSE

[P7] PCR NoticeAlicensetoperformthepatented5'NucleaseProcessforresearchisobtainedbythepurchaseof(i)bothAuthorized5'NucleaseCoreKitandLicensedProbe,(ii)aLicensed5'NucleaseKit,or(iii)licenserightsfromAppliedBiosystems.

ThisproductisanAuthorized5'NucleaseCoreKit.UseofthisproductiscoveredbyoneormoreofthefollowingUSpatentsandcorrespondingpatentclaimsoutsidetheUS:5,079,352,5,789,224,5,618,711,6,127,155,5,677,152,5,773,258,5,407,800,5,322,770,5,310,652,5,210,015,5,487,972,andclaimsoutsidetheUScorrespondingtoUSPatentNo.4,889,818.Thepurchaseofthisprod-uctincludesalimited,non-transferableimmunityfromsuitundertheforegoingpatentclaimsforusingonlythisamountofproductforthepurchaser'sowninternalresearch.SeparatepurchaseofaLicensedProbewouldconveyrightsundertheapplicableclaimsofUSPatentsNos.5,538,848,5,723,591,5,876,930,6,030,787,6,258,569,5,804,375(claims1-12only),and6,214,979,andcor-respondingclaimsoutsidetheUnitedStates.Norightunderanyotherpatentclaimandnorighttoperformcommercialservicesofanykind,includingwithoutlimitationreportingtheresultsofpurchaser'sactivitiesforafeeorothercommercialconsideration,isconveyedexpressly,byimplication,orbyestoppel.Thisproductisforresearchuseonly.DiagnosticusesunderRochepatentsrequireaseparatelicensefromRoche.FurtherinformationonpurchasinglicensesmaybeobtainedfromtheDirectorofLicensing,AppliedBiosystems,850LincolnCentreDrive,FosterCity,California94404,USA.

[L15] Hot Start PCRLicensedunderU.S.PatentNo.5.338,671and5,587,287,andcorrespondingpatentsinothercountries.

[L52] Rox Reference Dye (Research Field)Purchaseofthisproductincludesanimmunityfromsuitunderpatentsspecifiedintheproductinserttouseonlytheamountpurchasedforthepurchaser'sowninternalresearch.Nootherpatentrightsareconveyedexpressly,byimplication,orbyestoppel.FurtherinformationonpurchasinglicensesmaybeobtainedbycontactingtheDirectorofLicensing,AppliedBiosystems,850LincolnCentreDrive,FosterCity,California94404,USA.

[M57] LA TechnologyThisproductiscoveredbytheclaims6-16ofU.S.PatentNo.5,436,149anditsforeigncounterpartpatentclaims.

TaqMan®The5'nucleaseprocessiscoveredbypatentsownedbyRocheMolecularSystems,Inc.andF.Hoffmann-LaRocheLtd.Purchaseoftheproductdoesnotprovidealicensetousethispatentedtechnology.

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NOTE : Thisproductisforresearchuseonly.Itisnotintendedforuseintherapeuticordiagnosticproceduresforhumansoranimals.Also,donotusethisproductasfood,cosmetic,orhouseholditem,etc.Takaraproductsmaynotberesoldortransferred,modifiedforresaleortransfer,orusedtomanufacturecommercialproductswithoutwrittenapprovalfromTAKARABIOINC.Ifyourequirelicensesforotheruse,pleasecontactusbyphoneat+81775437247orfromourwebsiteatwww.takara-bio.com.

Cat. #RR039A Premix Ex Taq™ (Perfect Real Time) … · Table of contents I. Description ......

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