Carl Zeiss MicroImaging AIS

Solutions for DigitalPathology

Andrew LesniakDirector, Product Management

UpdateZeiss, Trestle, Clarient (ACIS)

The Device technology group was acquired from Clarient Inc., by Carl Zeiss in March 2007. Combined installation base of 600+ Units worldwide.

Full acquisition – including both the Trestle and the ACIS family of products

New entity formed under Carl Zeiss – Carl Zeiss MicroImaging AIS

Commitment to the technology portfolio, including:

- Integration into the Carl Zeiss Support structure (worldwide).

- Committed R&D investments for technology enhancement.

- Interoperability with the existing Zeiss product portfolio; including Image Acquisition, Mirax™ Platforms, and advanced technologies such as the PALM MicroBeam platform for Genomic and Phenotype applications.

Light Microscopy

macro

Advanced Imaging Microscopy Digital Pathology Solutions

Classical Technologies Imaging Laser Digital Detection

cellular

molecular

Technology

Applications For Digital Pathology Information Management:

•Leading Computer Aided Diagnostics and Analysis

•AP Workflow and Educational Data Management

Leveraging a Leading Product Portfolio for a

Complete Pathology Imaging Solution

Scalable Product Constellation

• Small footprint

• 20x Plan-Apo objective with 0.8 NA

• Image capture from barcode area w/ barcode identification

• Optional 40x Plan-Apo objective with 0.95 NA

• Choice of different camera adaptors (1x or 0.63x)

• Optovar option (1.25x and 1.6x)

• Fluorescence option

Resolution range from 0.11 µm/pixel ... 0.52 µm/pixel

MIRAX Lab Automation Digital Pathology Solution

• Zeiss Microscope Platform and Optics Configuration

• Full Robotic Telepathology functionality, including focal control, condenser settings

• Scalable to Digital Whole Slide Acquisition with user selectable Objective (4, 10, 20, 40, 60)

• Multi-focal plane imaging and robust focus technology ensures for robust imaging capability.

• Automated Slide Handling of 1, 4, 50 slides

• Fluorescence Multi-Channel Capable

MIRAX Micro Robotic Digital Pathology Solution

Overlay Extended FocusReal-Time Acquisition of

image series from different focus positions

Unique Scanning Advancements: MicroField Acquisition

Multi channel FluorescenceImage acquisition in several individual fluorescence channels

DAPI

Overlay

TexasRed

FITC

Scanning Advancements: Multi-channel

Fluorescence Acquisition

Digital Pathology Analysis:

Innovations with Imaging

Tissue Scoring and Quantitative Analysis Membrane StainsNuclear stainsIntegrated Optical Density

Rare Event Detection Sentinel Lymph nodesBone marrow

Object Detection and CountingNuclear Cell Counting,Micro-Vessel Density, Ploidy

Tissue Micro Array Scoring

Red blood cells

ICC-negative lymphoid cells

ICC-positive lymphoid cells

ICC-negative myeloid cells

ICC-positive myeloid cells

Size Blue Content Brown Contentsmall none/low medium/high

small high none/low

small high medium/high

large high none/low

large high medium/high

Digital Pathology Analysis:

Cellular Classification in Cytospin®

Workflow, Educational and Reference Materials

Virtual Lab creation- organization ofDigital Images by context

Linking a slide to relevant accession,histological and pathological contextinformation

Linking areas of interest on a slideto relevant teaching or lesion specificinformation

Preservation of compound relatedeffects for future reference

Digital Pathology Workflow:

Enabling Scale

We make it visible