Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO
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Transcript of Carl Henrik Gørbitz, Ute Krengel Department of Chemistry, UiO
Carl Henrik Gørbitz, Ute KrengelDepartment of Chemistry, UiO
Crystal engineering
Protein crystallography
Biomarker identification
Molecules
H2
ethanesucrose
Molecules
Molecules
Crystal
Crystal
”Crystal engineering”, we make a supermolecule!
Building blocks
nodelinker
Problem 1
Molecules are very small, hard to
put together one by one
Xe-atoms on Ni-surface (Don Eigler, 1989)
Problem 2
As they are so small, we must put together very many before we reach a macroscopic size
A 1 x 1 x 1 mm sugar crystal contains 1,4 · 1018 molecules
Building blocks
Building blocks
Glue
Intermolecular forces
Must be: strong Directional
Two main types used in CE: Hydrogen bonds Metal coordination
MOFs
“Metal-Organic Frameworks (MOFs) are crystalline compounds consisting of metal ions or clusters coordinated to often rigid organic molecules to form one-, two-, or three-dimensional structures that can be porous” (from Wikipedia)
MOF-5
Can we do this without metal ions?
Guanidinium derivatives
Fumaric acid complex
CN
N
NH
H
H
H
H
H
+
Guanidinium derivatives
DNA-based systems
DNA-based systems
DNA-based systems
Applications
Construction of polar materials for non-linear optics molecular magnets porous materials for storage molecular sieves sensors molecule traps biological model systems catalysts and much more
Biomarker identification
Biomarker: A biological molecule found in blood, other body fluids, or tissues that is a sign of a normal or abnormal process, or of a condition or disease. A biomarker may be used to see how well the body responds to a treatment for a disease or condition. Also called molecular marker and signature molecule
Present in small amounts = hard to identify
Biomarker identification
Nano-HPLC-system
Group
Carl Henrik
Fassil
Lianglin
Malgorzata
Lise-Lotte
StevenOla
Protein crystallography
Structure/Function Studies of Structure/Function Studies of Medically Relevant SystemsMedically Relevant Systems
Department of Chemistry
Ute Krengel
University of Oslo
X-ray crystallography
Complementary techniques (molecular biology, protein chemistry, ligand binding studies, cell biology, molecular docking, organic chemistry)
MethodsMethods
Glycobiological Targets◦Mucins◦Bacterial Toxins◦Bacterial Adhesins◦Anti-tumor Antibodies◦Mushroom Lectins
Enzymes◦Chorismate mutases
GlycoNor
TargetsTargetsProtStru
ct
Glycobiological Targets:◦Bacterial Toxins – Structure and Function
Investigation of blood group dependence Delivery mechanisms? Drug design
◦Anti-tumor Antibodies for Immunotherapy Recombinant production, crystallization and X-ray
structure determination
Masters ProjectsMasters Projects
GroupGroup
Ute
Øyvind
Hedda
DanielJulie
Dipankar
Dani