8/14/2019 Cardiolipin Antibodies Screen

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RESULTS

WELL SAMPLE

OPTICAL

DENSITY ASSESSMENT

A CALIBRATOR 0.199 -

BNEGATIVE

CONTROL0.003 -

C SAMPLE 2 0.147 -

D SAMPLE 2 0.001 -

E SAMPLE 3 0.000 -

F SAMPLE 4 0.001 -

G SAMPLE 5 0.054 -

CALCULATION OF RESULTS

Results are interpreted qualitatively by calculating a cut-off value (A)

or semi quantitatively by calculating the binding index (BI) for each

sample (B) on the basis of the cut-off determined:

o ODcalibrator x FACTOR = OD cut-off

The factor for calculation is stated in the control certificate provided

in the kit. The factor value may vary from lot to lot.

o (A) Example for the calculation of the cut-off value:

ODcalibrator = 1.214

factor = 0.15

ODcut-off= 1.214 x 0.15 = 0.182

o (B) For the calculation of the binding index (ratio) the following

formula should be applied:

BI = ODsample/ODcut-off

Example:

ODcut-off= 0.182 ODsample = 0.453

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BI = 0.453 / 0.182 = 2.5

DISCUSSION:Anti-Cardiolipin is an indirect solid phase enzyme immunoassay

(ELISA) for the simultaneous quantitative measurement of IgG, IgM and

IgA class autoantibodies against cardiolipin in human serum or plasma.

This assay is intended used for in vitro diagnostic in diagnosis of an

increased risk of thrombosis in patients with Systemic Lupus

Erythematosus (SLE) or lupus-like disorders.

Antiphospholipid syndrome (APS) is a systemic autoimmune disease

characterized by a thrombophilic state and by obstetrical complications.

The Scientific and Standardization Committee of the International Society

on Thrombosis and Hemostasis has issued consensus criteria that may be

used to help laboratory diagnosis. Accordingly, thrombophilic patients

should be screened both by phospholipid-dependent tests to detect lupus

anti-coagulant (LA) and by assaying for phospholipid antibodies with solid

phase ELISA tests to detect cardiolipin antibodies (aCl).

The presence of anti-cardiolipin antibodies in systemic lupus

erythematosus (SLE) can be related to the development of thrombosis and

thrombocytopenia, in gynaecology they are supposed to cause

intrauterine death or recurrent abortion. Furthermore, anti-cardiolipin

antibodies have been found in some non-thrombotic neurological disorders

like cerebrovascular insufficiency, cerebral ischemia or chorea and in

myocardial infarction.

Anti-Cardiolipin autoantibodies are found in the immunoglobulin

classes IgG, IgM and/or IgA. The determination of IgM antibodies is a

valuable indicator in the diagnosis of beginning autoimmune diseases,

whereas IgG antibodies will be found in progressive stages of manifested

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autoimmune disorders. The determination of IgA antibodies seems to have

a greater importance in the African-Caribbean population.

Quantitative measurements of anti-Cardiolipin antibodies, especially

IgG, are an important parameter with high specificity in therapy-

monitoring of SLE-secondary forms.

Indication for determination of anti-Cardiolipin antibodies:

- SLE - Thrombosis

- Thrombocytopenia - Cerebral Ischemia

- Chorea - Epilepsy

- Recurrent Abortion - Intrauterine Death

CONCLUSION:

On this experiment, there is no positive were found of all samples

detected.

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