"Can you hear that cell ?" - Patch clamp
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Transcript of "Can you hear that cell ?" - Patch clamp
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Georg Ohm (1789-1854)
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Georg Ohm (1789-1854)
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Example with potassium
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Bert Sackmann (left) and Erwin Neher (right) Nobel prize for patch-clamp (1991)
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In bath : current amplitude is directly dependant of pipette size On cell: current amplitude is directly dependent of seal quality In whole-cell mode: Current amplitude is directly dependent of cell RESISTANCE Current decay (and charge) properties are directly dependent on cell morphology/size
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In bath : current amplitude is directly dependant of pipette size On cell: current amplitude is directly dependent of seal quality In whole-cell mode: Current amplitude is directly dependent of cell RESISTANCE Current decay (and charge) properties are directly dependent on cell morphology/size
![Page 15: "Can you hear that cell ?" - Patch clamp](https://reader030.fdocuments.us/reader030/viewer/2022020218/55b9c98abb61eb2b388b4763/html5/thumbnails/15.jpg)
In bath : current amplitude is directly dependant of pipette size On cell: current amplitude is directly dependent of seal quality In whole-cell mode: Current amplitude is directly dependent of cell RESISTANCE Current decay (and charge) properties are directly dependent on cell morphology/size
![Page 16: "Can you hear that cell ?" - Patch clamp](https://reader030.fdocuments.us/reader030/viewer/2022020218/55b9c98abb61eb2b388b4763/html5/thumbnails/16.jpg)
In bath : current amplitude is directly dependant of pipette size On cell: current amplitude is directly dependent of seal quality In whole-cell mode: Current amplitude is directly dependent of cell RESISTANCE Current decay (and charge) properties are directly dependent on cell morphology/size
![Page 17: "Can you hear that cell ?" - Patch clamp](https://reader030.fdocuments.us/reader030/viewer/2022020218/55b9c98abb61eb2b388b4763/html5/thumbnails/17.jpg)
In bath : current amplitude is directly dependant of pipette size On cell: current amplitude is directly dependent of seal quality In whole-cell mode: Current amplitude is directly dependent of cell RESISTANCE Current decay (and charge) properties are directly dependent on cell morphology/size
![Page 18: "Can you hear that cell ?" - Patch clamp](https://reader030.fdocuments.us/reader030/viewer/2022020218/55b9c98abb61eb2b388b4763/html5/thumbnails/18.jpg)
In bath : current amplitude is directly dependant of pipette size On cell: current amplitude is directly dependent of seal quality In whole-cell mode: Current amplitude is directly dependent of cell RESISTANCE Current decay (and charge) properties are directly dependent on cell morphology/size
![Page 19: "Can you hear that cell ?" - Patch clamp](https://reader030.fdocuments.us/reader030/viewer/2022020218/55b9c98abb61eb2b388b4763/html5/thumbnails/19.jpg)
In bath : current amplitude is directly dependant of pipette size On cell: current amplitude is directly dependent of seal quality In whole-cell mode: Current amplitude is directly dependent of cell RESISTANCE Current decay (and charge) properties are directly dependent on cell morphology/size
![Page 20: "Can you hear that cell ?" - Patch clamp](https://reader030.fdocuments.us/reader030/viewer/2022020218/55b9c98abb61eb2b388b4763/html5/thumbnails/20.jpg)
In bath : current amplitude is directly dependant of pipette size On cell: current amplitude is directly dependent of seal quality In whole-cell mode: Current amplitude is directly dependent of cell RESISTANCE Current decay (and charge) properties are directly dependent on cell morphology/size
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