C9 - Aparat Golgi UTM 2015

8/19/2019 C9 - Aparat Golgi UTM 2015

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APARATUL GOLGI

CAMILLO GOLGI 1843-1926


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APARATUL GOLGI

• Organit delimitat de endomembrane,organizate de stie de !isterne re!"rbate,!" rol in mat"rarea, sortarea si e#$ediereama!romole!"lelor !atre destinatia %nal&

• Asem&n&tor membranei !el"lare, !on'inere!e$tori (i $roteine de trans$ort

• )arti!"laritatea l"i !onst& *n !on'in"t"l "ni!

de enzime din l"men"l organit"l"i


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+LA.+C+A A)A+L+I GOLGI


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3-D model of the Golgi region.

Marsh B J et al. PNAS 2001;!"23-2#0$©2001 by National Academy of Sciences


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Jennifer Lippincott-Schwartz Lab


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-im"no/"ores!en'&

000!ellsignal!om

http://www.google.ro/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&docid=Dllyevn8uhQ-yM&tbnid=WvtE-8yr47EwGM:&ved=0CAQQjB0&url=http://www.cellsignal.com/catalog/organelle.html&ei=hnGDUvYth8uyBt32gIgJ&bvm=bv.56343320,d.Yms&psig=AFQjCNGUcA7e8M-XjDwHN7luyzAmRfHCoA&ust=1384431907593134http://www.google.ro/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&docid=Dllyevn8uhQ-yM&tbnid=WvtE-8yr47EwGM:&ved=0CAQQjB0&url=http://www.cellsignal.com/catalog/organelle.html&ei=hnGDUvYth8uyBt32gIgJ&bvm=bv.56343320,d.Yms&psig=AFQjCNGUcA7e8M-XjDwHN7luyzAmRfHCoA&ust=1384431907593134


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OGAIAA A)AA+L+IGOLGI

• Li$ide membranare 5 $arti!i$& laorganizarea de mi!rodomenii

• )roteine membranare – matri!eale

– re!e$tori s$e!i%!i M6)

– trans$ortori

– nzime s$e!i%!e gli!ozil-transferaze,n"!leozid-difosfataze


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POLARITATEA APARATULUI GOLGI

http://var/www/apps/conversion/tmp/scratch_2/Golgi%20Apparatus_cut1.mp4http://var/www/apps/conversion/tmp/scratch_2/Golgi%20Apparatus_cut1.mp4


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POLARITATE

• )ro$rietatea "nor str"!t"ri de a aeadoi $oli di7eri'imor7ologi! (i sa" bio!imi!


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Funcţiile complexului Golgi

1 Glicozilarea proteinelor !glicozilare

terminal" a structurilor N #glicozi$ice% formarea structurilor O#glicozi$ice&'

( )aturarea proteinelor !glicozilare%

sulfatare% fosforilare% proteoliz"&'* Fosforilarea manozei +n enzimelelizozomale% ,iogeneza lizozomilor'

- .ortarea /i transportul,iomoleculelor la $estinaţia 0nal"'

Pro$ucerea glicozaminoglicanilor'2 Prelucrarea s0ngolipi$elor

!s0ngomielina% glicolipi$ele

1:


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GLI3O4ILAREA

O#GLI3O4ILAREA 5#GLI3O4ILAREA

! *n $a(i s"!!esii, *n an"mite !om$artimente ale a$arat"l"


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GLI3O4ILAREAPROTEI5ELOR

• O-GLICOILAA • -GLICOILAA

A.

Man

Man Man

A!Gl"

A!Gl"

Man

Man

Man

Man

Gl"

Gl"

Man

Man

Gl"

. ;O-

L


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GLI3O4ILAREA

)olecular 3ell 7iolog8 -t9 e$ition Lo$is9 6% 7er: A% 4ipurs:8 .L% et al

1

2


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• )ar!"rs"l $roteinei *n !om$artiment"l *n !are treb"ie=


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C"m a>"nge $roteina *n di7eritele!om$artimente ale A$arat"l"i Golgi=

• )o$elul matur"rii cisternelor

http://var/www/apps/conversion/tmp/scratch_2/model%20maturare%20cisterne.mp4http://var/www/apps/conversion/tmp/scratch_2/model%20maturare%20cisterne.mp4


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Model"l trans$ort"l"i ezi!"lar

După: Storrie B !epper"o" # Nilsson $ – Trends in Cell Biology 10 %&'(%)1 *2000+

Models for intra Golgi traffi%&ing


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Models for intra-Golgi traffi%&ing.

Ja%&son ' ( J 'ell S%i 200;122"##3-#)2©200) by $,e -ompany of Biolo.ists /td


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integrati;- 2 !&i anterogra$e de trans$ort lent, $emodel"l mat"rarii!isternelor (i ra$id, $rinzone de 7"zi"ne *ntre!isternele *ne!inate,!are s& $ermit& tre!ereadintr-"n !om$artiment

*n !el&lalt

( 3 ti$"ri de transportretrogra$<

( .e!torial 5 ndozomal 5 G (i cis-

( )rin di7"zi"neretrograd& a$roteinelor rezidente

Golgi( La distan & G-

r"!!o et al, at Cell ?iol, 2::4


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.!o$"l gli!ozil&rii

• @ormarea gli!o!ali#"l"i


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2:

Antigenele de gr"$ sang"in

/odis, et al olecular cell biolo.y 3 4reeman 5 -o6 1)))

NAc7al transfera8a 7al transfera8a

/e.enda

.luco8a

.alacto8a

fuco8a

NAc7luco8amina


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• Gli!ozilarea $entr" $rote!'ie *m$otria degrad&rii enzimati!e

http://var/www/apps/conversion/tmp/scratch_2/The%20glycosylation%20of%20antibodies%20-%20YouTube_cut.mp4http://var/www/apps/conversion/tmp/scratch_2/The%20glycosylation%20of%20antibodies%20-%20YouTube_cut.mp4http://var/www/apps/conversion/tmp/scratch_2/The%20glycosylation%20of%20antibodies%20-%20YouTube_cut.mp4http://var/www/apps/conversion/tmp/scratch_2/The%20glycosylation%20of%20antibodies%20-%20YouTube_cut.mp4http://var/www/apps/conversion/tmp/scratch_2/The%20glycosylation%20of%20antibodies%20-%20YouTube_cut.mp4http://var/www/apps/conversion/tmp/scratch_2/The%20glycosylation%20of%20antibodies%20-%20YouTube_cut.mp4http://var/www/apps/conversion/tmp/scratch_2/The%20glycosylation%20of%20antibodies%20-%20YouTube_cut.mp4http://var/www/apps/conversion/tmp/scratch_2/The%20glycosylation%20of%20antibodies%20-%20YouTube_cut.mp4http://var/www/apps/conversion/tmp/scratch_2/The%20glycosylation%20of%20antibodies%20-%20YouTube_cut.mp4http://var/www/apps/conversion/tmp/scratch_2/The%20glycosylation%20of%20antibodies%20-%20YouTube_cut.mp4


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.OAA I IC;AA

• ?IOGA LIOOMILO

http://var/www/apps/conversion/tmp/scratch_2/Protein%20Modification%20(Golgi)%20-%20YouTube_cut.mp4http://var/www/apps/conversion/tmp/scratch_2/Protein%20Modification%20(Golgi)%20-%20YouTube_cut.mp4


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.ORTAREA =I TRA5.PORTULPROTEI5ELOR LA >E.TI5A?IA FI5AL@#

)E3A5I.)E

1 .emnal"l de sortare rezid& *n *ns&(i

str"!t"ra $roteinei2 .ortare !atre $ol"l a$i!al $rin an!ora

G)I

3 rans$ort nedi7erentiat si retinere lalo!"l de destinatie de $roteine lo!ale

4 e!r"tarea an"mitor $roteine ada$tor la

inelis"l ezi!"lar 24


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TRAFI3UL >E E4I3ULE

• 2 !om$artimente – Cel de biosintez& -Golgi intermediate

!om$artment BGIC 5 !is Golgi

– Com$artiment"l endo!itar G 5membrana !el"lar& - endo!Dti! re!D!ling!om$artment BC

- Anterograd( etrograd


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TRAFI3UL >E E4I3ULE

• )roteine transmembranare Golgi• Coatomeri• )roteine G mi!i

@ormareaezi!"lelor

• )roteine G mi!i• lemente de !itosEelet rans$ort

• )roteine transmembranare Golgi• )roteine G mi!i

An!orare la

membrana'int&

• )roteine .A• )roteine G mi!i@"zi"ne

http://var/www/apps/conversion/tmp/scratch_2/Golgi/Clathrin%20-%20YouTube.mp4http://var/www/apps/conversion/tmp/scratch_2/fuziune%20membrane.JPGhttp://var/www/apps/conversion/tmp/scratch_2/fuziune%20membrane.JPGhttp://var/www/apps/conversion/tmp/scratch_2/fuziune%20membrane.JPGhttp://var/www/apps/conversion/tmp/scratch_2/Golgi/Clathrin%20-%20YouTube.mp4http://var/www/apps/conversion/tmp/scratch_2/Golgi/Clathrin%20-%20YouTube.mp4


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TRAFI3UL >E E4I3ULE # FU4IU5E

Leab" M, JCMM, 2::6


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e!ir!"lare de$roteine$ro$riiorganit"l"i deorigine

- Fezi!"le CO)

I

rans$ort"l$roteineidestinate!i!l"l"ise!retor

- Fezi!"le

CO)II- Fezi!"le!latrin&

, r a n s $

o r t r e t r o g

r a d

,rans

$ortante

r

ograd

A)7ELE >EPI5> >E PROTEI5E G )O5O)ERI3E


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3I3LUL .E3RETOR>rumul parcurs $e o protein" $e la

sintez" pBn" la $estinaţia 0nal"• es!ris de G )ALA, 7olosind a"toradiogra%a

• A)

RER ERGI3 GOLGI # )P

Lippincott#.c9Cartz D, )air Ann" e ?io$Ds2:1:H399-J8


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3I3LUL .E3RETOR

Li$$in!ott-.!0artz Lab

http://var/www/apps/conversion/tmp/scratch_2/Secretory%20Pathway%20(ER%20to%20PM)%20-%20YouTube.mp4http://var/www/apps/conversion/tmp/scratch_2/Secretory%20Pathway%20(ER%20to%20PM)%20-%20YouTube.mp4


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3I3LUL .E3RETOR

• .e!re'ie !ontin"& B!onstit"ti&

• .e!re'ie stim"lat& 5 – e$ozitarea ezi!"lelor *n !ito$lasm&

– Cel mai 7re!ent stim"l este !re(tereaCa !itosoli!

C9 - Aparat Golgi UTM 2015

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