by

Ivo Frébort

Protein electrophoresis

Polyacrylamide chemistryPolyacrylamide chemistry

acrylamide

3-15 %T

N,N’-methylene-bis-acrylamide

5 %C

polyacrylamide

S2O82- 2 SO4

-.

TEMED ( N, N,N´,N´-tetramethylethylendiamin)

Gel preparationGel preparation

Protein visualizationProtein visualization

ABS

600500400

Wavelength - nm

Coomasie bluewith proteinsbound

700

Coomasie blue

ABS

600500400

Wavelength - nm

Coomasie bluewith proteinsbound

700

Coomasie blue

Sensitivity 500 ng/band

Sensitivity 10-50 ng/band

Comassie blue stainig Silver stainig

CH2

N CH2

N CH2

R

CH2

N

n

n

R2

n = 2 nebo 3 R = H nebo -(CH2)n-COOH