By: Haben M. & Cordelia E.

BACKGROUND These 2 brothers were born in Munich,

Germany Their father was a professor of forensic

medicine Hans was the oldest of both He was a physician who researched

blood proteins that can destroy bacteria Eduard: chemist; researched yeast cells

along side Adolf von Baeyer

What was already known? That food spoiled after a few days when

exposed to air The fermenting agent comes from the air The cause: bacteria The theory of fermentation was first

developed by Louis Pasteur He believed that fermentation was

physiological in yeast cells In contrast Justin von Liebig, a chemist,

believed that the cause was a chemical agent within the cell

The Experiment:

Hans Buchner worked along side his younger brother by contributing the study of anaerobic bacteria

He discovered the blood serum could kill bacteria,

But this ability was useless if heated Anaerobic bacteria: bacteria that can

exist with limited or complete absence of oxygen

Summarize: Eduard Buchner used a pestle & mortar to

ground up yeast Then extracted juices The "dead" liquid fermented a sugar

solution, which formed carbon dioxide & alcohol much like living yeasts.

The “non-living“ yeast behaves just like the cells

It was then acknowledged that fermentation is caused by an enzyme

Cell-free Fermentation:

Buchner continued Pasteur’s discoveries

Through a series of experiment he discovered the enzyme: ZYMASE

This enzyme catalyzes the reaction of fermentation to produce C2H5OH and CO2

Proved that yeast cells were not needed

Relation to cellular respiration:

Ferments glucose Produces ethanol and carbon dioxide in

the pyruvate stage Fermentation is an added cycle that

replaces the Krebs cycle There is no need for oxygen

What was learned?

Zymase catalyses the breakdown of glucose in the process of glycolysis

Eventually slows down the process Yeast can breakdown sugars with or

without oxygen Enzymes can be extracted from yeast Final products: alcohol & carbon dioxide

References: Eduard Buchner. (n.d.). Retrieved November 15,2010 from

http://www.nndb.com/people/227/000099927/ "Eduard Buchner - Biography". Nobelprize.org. 16 Nov 2010

http://nobelprize.org/nobel_prizes/chemistry/laureates/1907/buchner-bio.html

Cell- free fermentation. (n.d.). Retrieved November 15,2010 from http://nobelprize.org/nobel_prizes/chemistry/laureates/1907/buchner-lecture.pdf

The background to Eduard Buchner's discovery. (n.d.) Retrieved November 15, 2010 from http://www.jstor.org/pss/4330549

Zymase. (n.d.). Retrieved November 15, 2010 from http://www.answers.com/topic/zymase

Eduard Buchner. (n.d.). Retrieved November 15,2010 from http://www.britannica.com/EBchecked/topic/82903/Eduard-Buchner

Cellular Respiration & Fermentation. (n.d.). Retrieved November 16, 2010 from http://biology.clc.uc.edu/Courses/bio104/cellresp.htm

THE END