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M135

Perfusion ControllersEight Channel Perfusion Valve Control Systems

VC-8 Perfusion Valve ControlWarner perfusion valve control systems areuncomplicated and easy to operate.

The VC-8 Valve Controller is configured tocontrol up to 8 valves. Each valve is individ-ually accessed by either a manual switch, anexternal analog signal, or an external TTLsignal. A spill sensor probe is included fordetecting an overflow condition in your per-fusion chambers and will shut off all valvesthereby protecting your valuable microscopeoptics. An event-marker pulse, generatedeach time a valve is switched on, is providedfor output to tape or chart recorders.

Systems include all components necessary to get set up and running in very little time. Pinchvalve and Teflon™ valve systems include the VC-8 Controller, 8 valves, a valve bracket with 8 ft.cable and MP-8 Series Manifold. Pinch valve systems are supplied with C-Flex™ valve tubing.Included with the Pinch and Teflon™ Valve Systems are eight 60 cc reservoirs, a reservoir hold-er and support stand, stopcocks and tubing connectors. Pinch valve systems are also suppliedwith 50 ft. of Tygon™ tubing. Systems with a Teflon™ valve include 25 ft. of Teflon™ tubing.

The VC-8M Mini-Valve Perfusion Control System includes the VC-8 Controller, 8 valves, a valvemanifold with 8 ft. cable and an ML-8 Series Manifold. Also included are eight 10 cc reservoirs,a reservoir holder, ring stand, stopcocks, tubing connectors and 100 ft. of PE-50 tubing.

VC-8P Pinch Valve SystemsPinch valves are the simplest to maintain as the solution never comes in contact with the valveand tubings are easily changed. Valves are dual acting (3-way) with both normally open andclosed sides. Y connectors at the valve inputs permit solution flow to waste with the valves off.

VC-8T Teflon™ Valve SystemsTeflon™ valves are available for applications where resistance to chemicals is a concern. Thevalves are 2-way (either on or off).

VC-8M Mini-Valve SystemsThe VC-8M is designed for slow flow perfusion systems where smaller diameter tubing isused. Eight valves mount directly to a compact Delrin manifold. The 3-way valves allow forsolutions to flow to waste if desired. The VC-8M system is ideally suited for use with the SF-77B Perfusion Stepper Systems.

Catalog No. $ Model ProductBS4 64-0185 2,495.00 VC-8P Valve Control System/8 pinch valves

BS4 64-0186 2,495.00 VC-8M Valve Control System/8 mini valves

BS4 64-0187 2,795.00 VC-8T Valve Control System/8 Teflon valves

• Spill sensor - an auto shut off probefor use with inverted microscopes

• Complete systems with choice of 3valve types:

- Pinch valves

- Teflon™ valves

- Miniature Lee saline valves

• Manual or computer control

• Low noise

• Low self-heating design

VC-8P Pinch Valve Systems

VC-8T Teflon® Valve Systems

VC-8M Mini-Valve Systems

Mini Valves

11,000 Specialty Products to Enhance Your BioresearchU.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com

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M13611,000 Specialty Products to Enhance Your Bioresearch

U.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com

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Perfusion Controllers

VC-6 Valve Controller

SpecificationsValves:

VC-6 Standard 12 V 3-way pinch valvesVC-6 Teflon® 12 V 2-way Teflon™

VC-6M Mini 12 V 3-way solenoid valves

Typical Max. Flow Rates (with 60 cm head):VC-6 Systems 10 ml/minVC-6M Systems 1 ml/min

VC-6 Valve Controller:Switching Manual or TTL logicEvent Marker Logic level pulsePower 110-130 or 200-250 VAC, 50/60 Hz, 50 VASize 8.9 x 20 x 25 cm (H x W x D)

Warranty All systems carry 2-year warranty

Perfusion Valve Control Systems VC-6 and VC-6M

Perfusion Valve ControlWarner perfusion valve controlsystems are uncomplicated andeasy to operate. The VC-6 ValveController is configured to con-trol up to 6 valves. Each valve isindividually controlled by a man-ual switch or an external digital(TTL) signal. An event markerpulse, generated each time avalve is switched on, is providedfor tape or chart recordings.

VC-6 Standard Pinch Valve SystemsStandard systems are supplied with pinch valves. They are the sim-plest to maintain as the solution never comes in contact with thevalve and tubings are easily changed. Valves are dual acting (3-way)with both normally open and closed sides. A Y connector at the valveinput permits solution flow to waste with the valve off.

VC-6 Teflon™ Valve SystemsTeflon™ valves are also available and are for applications whereresistance to chemicals is a concern. The valves are 2-way (eitheron or off).

VC-6M Mini-Valve SystemsThe VC-6M is designed for slow flow perfusion systems where small-er diameter tubing is used. Up to six valves mount directly to a com-pact Teflon™ manifold. The 3-way valves allow for solutions to flowto waste if desired. The VC-6M system is ideally suited for use withthe SF-77B perfusion stepper systems.

• Manual or computercontrol

• Modular: 2, 4 or 6channels

• Expandable to 6 channels

• Basic or complete systems

• Pinch valves, standard

• Teflon valves, optional

Valve Control Systems VC-6 (left) and VC-6M

System Choices, Basic or CompleteThe VC-6 Valve Perfusion Control System is available in two configu-rations with a choice of either a standard pinch valve or Teflon™

Valve. The Basic Systems include the VC-6 Controller, Valves, ValveBracket with 8 ft long Cable and MP Series Manifold. The systemswith the standard pinch valve are also supplied with C-Flex™ ValveTubing.

The Complete VC-6 Systems includes all of the components in theBasic Systems plus six 60cc Reservoirs, Reservoir Holder, RingStand, Stopcocks and Tubing Connectors. In addition to that, the sys-tems with a standard pinch valve are supplied with Tygon™ Tubing(1/32 in x 50 ft, ID x L). The systems with a Teflon™ valve includeTeflon™ tubing (1/16 in x 25 ft, ID x L).

The VC-6M Mini-Valve Perfusion Control System is also available intwo configurations. The Basic Systems include the VC-6 Controller,Valves, Valve Manifold, 8 ft Cable and ML Series Manifold. TheComplete Systems include all of the components in the Basic Systemsplus eight 10cc Reservoirs, Reservoir Holder, Ring Stand, Stopcocks,Tubing Connectors and two packages of PE-50 Tubing (50 ft long).

All Systems configurations are offered with a choice of 2, 4 or 6valves. Systems ordered with less than 6 valves may be expanded at alater date.




M137 11,000 Specialty Products to Enhance Your BioresearchU.S. Toll Free: (800) 272-2775 • Fax: (508) 429-5732 • Online: www.harvardapparatus.com

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Perfusion ControllersPerfusion Valve Control Systems VC-6 and VC-6MCatalog No. $ Model ProductBasic Mini-Valve Perfusion Systems

BS4 64-0169 959.00 VC-62MBB Mini-Valve System, 2-Channel

BS4 64-0170 1,134.00 VC-64MBB Mini-Valve System, 4-Channel

BS4 64-0171 1,329.00 VC-66MBB Mini-Valve System, 6-Channel

Complete Mini-Valve Perfusion Systems

BS4 64-0172 1,147.00 VC-62MCS Mini-Valve System, 2-Channel



Additional Valves for VC-6M Mini Systems

BS4 64-0175 88.00 SV-1 Mini Solenoid Valve

Accessories for VC-6M Mini Systems

BS4 64-0143 154.00 SH-8/10 Syringe Holder with eight 10 ccSyringes

BS4 64-0165 36.00 SL-6 Stopcock with Luer Connector, pkg. of 6

Tubing for VC-6M Mini Systems

BS4 64-0752 13.00 PE-50/10 PE-50 Polyethylene Tubing, 10 ft L

BS4 64-0753 88.00 PE-50/100 PE-50 Polyethylene Tubing, 100 ft L

Basic Pinch Valve Systems

BS4 64-0127 959.00 VC-62BB Pinch Valve System with 2 Valves

BS4 64-0128 1,134.00 VC-64BB Pinch Valve System with 4 Valves

BS4 64-0129 1,329.00 VC-66BB Pinch Valve System with 6 Valves

Basic Teflon Valve Systems

BS4 64-0130 1,038.00 VC-62BBT Teflon™ Valve System with 2 Valves



Complete Pinch Valve Systems

BS4 64-0133 1,147.00 VC-62CS Pinch Valve System with 2 Valves



Complete Teflon Valve Systems

BS4 64-0136 1,226.00 VC-62CST Teflon™ Valve System with 2 Valves



Additional Valves for VC-6 Systems

BS4 64-0139 88.00 PV-1 Pinch Valve

BS4 64-0140 122.00 TV-2 Teflon™ Valve

Accessories for VC-6 Systems

BS4 64-0142 34.00 DC-6 Drip Chambers, pkg. of 6. Used to visu-ally observe the speed of perfusate.

BS4 64-0162 31.00 RS-1 Ring Stand

BS4 64-0163 154.00 SH-6/60 Syringe Holder with six 60 cc Syringes

BS4 64-0143 154.00 SH-8/10 Syringe Holder with eight 10 ccSyringes

BS4 64-0144 154.00 SH-6/140 Syringe Holder with six 140 cc Syringes

BS4 64-0164 18.00 TC-3 Tubing Connectors (1/16 in ID), pkg. of12 straight, 6 Y and 6 Luer Connectors

BS4 64-0165 36.00 SL-6 Stopcock with Luer Connector, pkg. of 6

Tubing for VC-6 Systems

BS4 64-0166 14.00 CFL-6 C-Flex (1/32 in x 6 ft, ID x L)

BS4 64-0167 25.00 TY-50 Tygon (1/16 in x 50 ft, ID x L)

BS4 64-0168 47.00 TT-25 Teflon™ (1/16 in x 25 ft, ID x L)

VC-6 Fast-Step Perfusion SystemThis perfusion system is thecombination of the VC-6Perfusion Valve Control Systemand the Fast Step PerfusionSystem. It allows computer

control of multiple perfusion setups, saving time and effort. Whenpurchased as a complete system, it also provides a discount. The system includes:

Catalog No. Model ProductBS4 64-0174 VC-66MCS Complete Mini-Valve System,

6-Channels

BS4 64-0114 SF-77B Perfusion Fast-Step System

BS4 64-0056 MM-33R Micromanipulator, Right Handed

BS4 69-0225 MB-B Magnetic Base

For Complete Details of the VC-6 Complete Mini-Valve PerfusionSystem, see facing page. For complete details on the Perfusion Fast-Step System, see pages M138 and M139.

Catalog No. $ Model ProductBS4 64-0177 4,476.00 VC-77SP Complete VC-6 Fast-Step

Perfusion System

BS4 64-0177 VC-6M Systemwith SF-77BStepper System

• Allows computer controlof multiple perfusionsetups

For SF-77B Perfusion Fast-Step System,see pages M138 and M139.

For Perfusion Chambers, Adapters and Platforms,see pages M112 to M123.

For Constant Flow Plastic Syringes,see pages M142.






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Perfusion ControllersPerfusion Fast-Step SF-77B

• Solution stimulus delivery for patch clamp and other electrophysiology studies

• Solution changes in milliseconds

• Minimal flow turbulence

• No switching through interveningsolutions

• Manual or automatic step control (digital or analog)

• Modest cost and easy maintenance

• Use with most chambers or culture dishes

The SF-77B is a simple but highly effectivestimulus solution delivery device for use in avariety of patch clamp and electrophysiologystudies. Control and test solutions flow contin-uously through adjacent stimulus deliverytubes and a stepper mechanism selects whichtube is directed at the preparation. The rapidresponse and nominal hysteresis of the steppermechanism allows for very fast switchingtimes. Complete solution changes are typicallyachieved within 20 msec for a large 700 mmstep and times are significantly shortened asthe step size is decreased.

Multiple Solution StudiesIn the standard configuration, up to six differ-ent solutions are connected to a single inputmanifold which in turn is connected to one ofthree square glass stimulus ports. The threestimulus port design is superior to a two-portdesign in that complex solution exchangeprotocols can be brought to bear on the sam-ple under study. Since the complete system isdesigned to accommodate three manifolds(one for each stimulus port) and each mani-fold can accommodate up to 6 feed lines, it ispossible to immediately select between 18different input solutions.

• Solution changes between stimulusports occur within milliseconds

• Changes between solutions connectedto individual port occur within 5 secs.

• Entirely new solutions can be addedinto any port with a waiting time ofno more than 30 seconds.

• Cell is never required to passthrough intervening solutions to getfrom control to test solution

Manual or External ControlThe stepper mechanism can be manually con-trolled via the front panel or externally direct-ed from your data acquisition program.Manually, the system can be stepped to 8 posi-tions in 7 equally spaced steps. These same 8positions can also be directly selected byapplying an analog signal to the external ana-log input BNC or by passing a 3 byte word tothe TTL input on the instrument rear panel.

Square Glass Stimulus PortsThe square glass tubes used for solutiondelivery significantly reduces mixing turbu-lence between solution flows allowing theSF-77B to be used for studies with bothmembrane patches and whole cells, evenwhen the cells are not fixed to a substrate.

System VersatilityThe design of the SF-77B permits the use ofvarious size glass tubing for perfusion delivery.

SF-77B: Standard System (0.7 mm ID tubes) The standard system is shipped with 3SG700-5 single-walled 3-barrel glass tubing whicheliminates the need to glue individual barrelstogether. Spacing between barrels is 0.7 mmand step speed between adjacent barrels istypically 20 msec. Single barrel SG800-5 tubes(up to 5) can be used with the same holder.

SF-77BLT:Large Tube System (1.0 mm ID tubes)Larger stimulus ports are required when usingthe SF-77B with larger cell structures such asthe Xenopus oocyte. Solutions are deliveredthrough 1.0 mm ID square tubes (SG1000-5)with barrel-to-barrel spacing of 1.4 mm.

SF-77BST:1 msec Stepping With Theta Tubing The SF-77B has been successfully testedusing theta style capillary tubing with a stepspeed of 1 msec between adjacent barrels.Standard 2 mm diameter theta tubing ispulled to a tip diameter of 300 mm with aspacing of 100 mm between barrels. In thisdesign, placement of the pipette tip is criti-cal due to the smaller perfusion stream.Since mechanical artifacts can be evidentwhen using these small step sizes the motordrive voltage is adjustable at the rear panel.

Easy Set-UpThe stepper mechanism is compact, light-weight, and free of either mechanical orelectrical noise. The mechanism connects tothe control box with a 2 meter shieldedcable and is provided with a mounting rodfor attachment to a manipulator. Manifoldscan support 2,4 or 6 inputs depending onthe experiment. Solutions flow from reser-voirs to the manifold through PE-50 tubingand PE-10 tubing is used to connect themanifold outputs to the glass tubes.





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Perfusion ControllersPerfusion Fast-Step SF-77B

Catalog No. $ Model ProductBS4 64-0114 2,403.00 SF-77B Standard System with MM Series

Manifolds, 1 pkg. 3SG700-5 Glass,GH-1 Glass Holder, and 1 pkg. eachPE-10 and PE-50 Tubing

BS4 64-0115 2,403.00 SF-77BST Theta Glass System with MM SeriesManifolds, 1 pkg. TGC-200-10 Glass,GH-2T Glass Holder, and 1 pkg.each PE-10 and PE-50 Tubing

BS4 64-0116 2,403.00 SF-77BLT Large Tube System with ML SeriesManifolds, 1 pkg. SG1000-5 Glass,GH-10 Glass Holder, and 2 pkg. ofPE-50 Tubing

Accessories

BS4 64-0119 47.00 3SG700-5 3-Barrel Square Glass Tubes, 0.6 mm x 5 cm (ID x L), pkg. of 10

BS4 64-0120 96.00 3SG700-10 3-Barrel Square Glass Tubes, 0.6 mm x 10 cm (ID x L), pkg. of 10

BS4 64-0121 29.00 SG-800-5 Single Barrel Square Glass Tubes, 0.6 mm x 5 cm (ID x L), pkg. of 25

BS4 64-0122 29.00 SG-1000-5 Single Barrel Square Glass Tubes, 1 mm x 5 cm (ID x L), pkg. of 25

BS4 64-0811 88.00 TG200-4 Theta Glass Tubes, 2.0 mm x 10 cm(OD x L), pkg. of 100

BS4 64-0124 77.00 GH-1 Glass Holder for 3SG700-5,3SG700-10 and SG800-5 Glass

BS4 64-0125 65.00 GH-2T Glass Holder for Theta Glass

BS4 64-0126 77.00 GH-10 Glass Holder for SG1000-5 Glass

Note: Model SF-77B/5M: SF-77B may also be ordered as a 5 manifold system. Consult ourtechnical support department for details.

SpecificationsNumber of Steps 1 to 7 (2 to 8 positions)

Step Size Adjustable from 100 µ m to 1.5 mm in 100 µ m increments,with STEPS/POSITION switch

Step Speed Typically 20 msec for 700 µ m step

Step Control:Manual 8 positions with POSITION selectorAnalog Signal 5 positions with voltage levels 0-4 V, 1V/stepDigital Signal 8 positions with 3 byte TTL signal

Max. Stepped Range 12.5 mm

Mounting Handle 6.3 mm X 10 cm (D x L)

Stepper Weight 110.5 g (including handle)

Solution Manifolds: Three manifolds supplied with each system; MM seriesfor SF-77B and SF-77BST and ML series with SF-77 BLT;Manifolds have 2, 4 or 6 inputs, specify your choice whenordering

MM Series MM-2, MM-4 and MM-6 manifolds use PE-50 tubing at input and PE-10 tubing at output

ML Series ML-2, ML-4 and ML-6 manifolds use PE-50 tubing at both input and output

Solution Flow Rates: Rates measured with solution reservoir height of approx.60 cm (24 in)

With MM Series 100 µ l/minWith ML Series 1 ml/min

Control Box:Size, H x W x D 6.3 x 14 x 23 cmPower Requirements 100 to 130 or 220 to 250 VAC, 50/60 Hz, 10 VA

System Shipping Weight 2.7 kg

Warranty 2 years, parts and labor

We designed the VC-6M to accompany the SF-77B stepper. It conve-niently fits inside a Faraday enclosure and is remotely controlled withthe VC-6 controller. When purchased with the SF-77B, the completepackage is discounted, see pages M136 and M137 for details.

These Fast-Step Systems are supplies with one 2 to 1 and two 6 to 1manifolds. Other manifolds are available separately, see page M140.

VC-6M Systemwith SF-77BStepper System,see page M137 for details

Glass Tubes (End Views)






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MPP SeriesThese manifolds are suitable forsystems in which solutions arepumped or at pressures greaterthan those generated in gravityfed systems. Input and outputports are 18 gauge stainless steelhypodermic tubing. PE-160 tub-ing slides over these ports tomake a snug fit.

MP SeriesMP series manifolds are recom-mended for gravity fed perfusionsystems. Input and output tubingare inserted with a friction fit.Manifolds should be ordered withinputs to match the number ofsolutions to be connected. Pinplugs to block unused inputs arealso supplied. MP series mani-folds are used with PE-160 tubing.

MM and ML SeriesDesigned to be part of the SF-77Perfusion Fast-Step systems,these miniature manifolds areuseful for applications involvingsmall volumes or slow flow rates.Small diameter tubing is usedwith these models; PE-50 tubingfor the input ports, and PE-10 orPE-50 tubing for the MM or MLseries output ports, respectively.

Perfusion Manifolds

Warner perfusion manifolds can be used in any application wherefrom 2 to 8 perfusion lines are required to be connected to a chamber or other device. Manifold inputs converge to the commonoutput as shown above with a minimum of dead space. They aredesigned for use with polyethylene (PE) tubing but can be usedwith any other tubing with similar dimensions, see PE tubing specifications below. When connected to a chamber via a shortlength of tubing, very rapid solution changes are possible.

SpecificationsMP Series MPP Series MM Series ML Series

Material Teflon® Delrin™ Delrin™ Delrin™

Large Diameter 9.4 mm 9.4 mm 8.0 mm 8.0 mm

Small Diameter 6.3 mm 6.3 mm 4.7 mm 4.7 mm

Body Length 22 mm 21 mm 18 mm 18 mm

Input Tubing PE-160 PE-160 PE-50 PE-50

Output Tubing PE-160 PE-160 PE-10 PE-50Note: Teflon and Delrin are trademarks of E.I. du Pont de Nemours & Co.

Output

Inputs

MP Series

MPP Series

MM Series

ML Series

Cat. No. $ Model InputsBS4 64-0206 67.00 MP-2 2

BS4 64-0207 67.00 MP-3 3

BS4 64-0208 67.00 MP-4 4

BS4 64-0209 78.00 MP-5 5

BS4 64-0210 78.00 MP-6 6

BS4 64-0211 89.00 MP-8 8Note: See below for specifications.

Cat. No. $ Model InputsBS4 64-0212 78.00 MPP-2 2

BS4 64-0213 83.00 MPP-3 3

BS4 64-0214 89.00 MPP-4 4

BS4 64-0215 95.00 MPP-5 5

BS4 64-0216 100.00 MPP-6 6

BS4 64-0217 111.00 MPP-8 8

BS4 64-0339 315.00 MPP-24 24Note: See below for specifications.

Cat. No. $ Model InputsBS4 64-0203 73.00 MM-2 2

BS4 64-0204 78.00 MM-4 4

BS4 64-0205 83.00 MM-6 6

BS4 64-0200 73.00 ML-2 2

BS4 64-0201 78.00 ML-4 4

BS4 64-0202 83.00 ML-6 6Note: See below for specifications.

MPP-24

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Exploded View of Base Mount and Several Add-on Sizes

Modular Syringe Holder

Catalog No. $ Model ProductBS4 64-0145 40.00 MSH/5M Syringe Base Mount,

5 cc

BS4 64-0146 40.00 MSH/10M Syringe Base Mount,10 cc





BS4 64-0151 37.00 MSH/5 Syringe Add-On, 5 cc






Accessories

BS4 64-0165 36.00 SL-6 Stopcock with LuerConnector, pkg. of 6

BS4 64-0162 31.00 RS-1 Ring Stand

BS4 64-0220 194.00 FR-50 Flow Valve

DN Series Constant FlowSyringes, see page M142

The modular syringeholder system offersusers the ability toassemble a family ofdifferently sized

syringes into a single apparatus. Warner Instrumentsoffers a full range of Add-On Holders to fit every application. Made from high quality Delrin and 316 stainless steel, this system assures excellent protectionfrom most harmful chemicals.

The modular syringe holder system consists of a singleSyringe Base Mount that is coupled with other Add-OnSyringe Holders. The Base Mount has a primary hole thatholds the first syringe. In addition to the primary hole, italso has a secondary hole that permits the unit to mounton an upright rod or ring holder ranging in size from 3/8to 5/8 inch. Both holes have a thumbscrew that tightensso that the rod or syringe is held securely in place.

Additional Add-On Syringe Holders can then mount oneither side of the Base Mount by simply snapping the Add-On onto the side of the Base Mount. Multiple Add-OnHolders can used and various sizes may be intermixed.

Fully Assembled ModularSyringe Holder

60 cc Base Mount

Upright MountingHole

• Designed to Fit 5, 10,20, 30, 60, and 140 ccSyringes

For Standard Plastic Syringes,see Pumps Section A.

Syringe MountingHole

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Perfusion ControllersDN Series Constant Flow Syringes

When using a perfusion system, a common difficulty fac-ing researchers is maintaining a constant flow as thereservoir empties. In general, since head pressure is alinear function of column height, the change in volumeof a 60 cc syringe as the solution reservoir drains canresult in a 20% decrease in flow rate.

Warner Instruments is pleased to introduce a simple andunique solution to this common problem. Our new DNSeries Constant Flow Syringes virtually eliminate changesin flow rate, normally to less than 1%. These reservoirsare designed to maintain a constant pressure on thesyringe contents without resorting to a complex or bulkyapparatus (such as a pressure system or regulator).

When used in conjunction with Warner Instruments’ FR-50 Solution Flow Valve, precise, sub-maximal flow con-trol is easily achieved. Syringe reservoirs may be refilledwithout interrupting solution flow. They are available in 5to 60 cc volumes and can be used with Warner’s MSHSeries Modular Syringe Holder System, see page M141.

Catalog No. $ Model ProductBS4 64-0157 38.00 DN/5 Constant Flow Syringe,

5 cc, pkg. of 6

BS4 64-0158 42.00 DN/10 Constant Flow Syringe,10 cc, pkg. of 6

BS4 64-0159 55.00 DN/20M Constant Flow Syringe,20 cc, pkg. of 6



Accessories

BS4 64-0220 194.00 FR-50 Flow Valve

BS4 64-0165 36.00 SL-6 Stopcock with LuerConnector, pkg. of 6

BS4 64-0162 31.00 RS-1 Ring Stand

BS4 64-0209 78.00 MP-5 Perfusion Manifold 5 to 1

DN SeriesConstant Flow Syringes

DN Series Constant FlowSyringes shown with ModularSyringe Holder, FR-50 FlowValve, Chamber, MP-5 Manifold and RS-1 Ring Stand

If you want to use the DN series of Constant FlowSyringe with the MSH Modular Syringe Holderseries, you must order the next size up in theseries. See the reference chart below for properordering information.

DN Series Constant Flow Syringes

Syringe Mount/ Base Add-On Size Holder Size Mount Holder

5 cc 10 cc BS4 64-0146 BS4 64-0152

10 cc 20 cc BS4 64-0147 BS4 64-0153

20 cc 60 cc BS4 64-0149 BS4 64-0155

30 cc 60 cc BS4 64-0149 BS4 64-0155

60 cc 140 cc BS4 64-0150 BS4 64-0156

See previous page for pricing and complete detailsFor standard plastic syringes, see Pumps Section A.

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Perfusion ControllersElectrode/Manifold Holders MHH-25 & MHH-38

The MHH-25 and MHH-38 Holders permit convenient mounting ofmanifolds and electrode holders or other devices with 6.3 or 9.5 mmdiameters. The holder head can be pivoted ±90° from the axial posi-tion and rotated 360° about the axis. Friction holds the head firmly inthe set position. Holder head and coupler are made from delrin®. Theanodized aluminum handle is 6.3 mm diameter x 6.3 cm long and willfit most positioners. The electrode/manifold holders are compatible withE and Q Series Electrode Holders (MHH-25) and PE Series and ThetaElectrode Holders MP and MPP Series Manifolds (MHH-38).

Catalog No. $ Model ProductBS4 64-0218 65.00 MHH-25 Holder for 6.3 mm (1/4 in) Devices

BS4 64-0219 65.00 MHH-38 Holder for 9.5 mm (3/8 in) Devices

FR-50 and FR-55S flow valves are used for fine control of solutions in agravity fed perfusion system or vacuum used to evacuate a chamber.When used for chamber evacuation, the FR-50 provides the fine vacu-um control critical to a properly operating system. Solution flow rate isadjustable from zero to a maximum of 10 ml/min. (measured with asolution head of 30 cm). Model FR-55S includes a convenient on-offswitch for interrupting the flow without disturbing the flow rate.

SpecificationsMaterials Teflon® and Delrin® Plastics

Input/Output Stainless Steel Tubing compatible with PE-160 Tubing

Catalog No. $ Model ProductBS4 64-0220 194.00 FR-50 Flow Valve

BS4 64-0221 260.00 FR-55S Flow Valve with On-Off Switch

Electrode/Manifold Holders Vacuum/Solution Flow Valve

Vacuum/Solution Flow Valve FR-50 & FR-55S

For Perfusion Manifolds,see page M140.

For E and Q Series of Electrode Holders,see pages M32 to M39.






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Perfusion ControllersHarvard Apparatus CO2 Gas/pH Controller

This compact stand-alone pH controller was designed to pre-cisely regulate the pH level of liquid biological solutions. It canbe used in a number of biological experiments requiringregulated pH, such as: isolatedorgans (heart, lung, kidney, etc.)or isolated tissues, see section Kin our full-line BioscienceCatalog.

Maintaining the pH is accom-plished by either ‘bubbling’ CO2

gas through the solution or flowing the CO2 gas over the surface of the solution or medium. To maintain the desired pH level, the ‘set-point’ of the controller isadjusted to the desired level. When the pH level exceeds the pro-grammed set point, the flow of acidifying CO2 gas is started. Adjustingthe controller’s flow rate regulator will determine at what rate the pHwill decrease, the higher the flow rate the faster the drop in pH. Thecontroller’s flow rate regulator will allow the end user to adjust the CO2

gas between 0 to 2.0 liters per minute. Once the pH reaches the setpoint, the gas flow automatically stops. Should the pH increase theprocess is repeated.

A three-position (manual override) switch on the front panel provides several ways to control the CO2 gas delivery.

By-Pass Mode: (up) the flow of CO2 gas is continuous and the flow rate will be controlled by adjusting the regulator.

Momentary Mode: (down) the flow of CO2 gas is allowed as long as the switch is depressed. The switch is spring loaded and will return to the Auto Mode when released.

Auto Mode: (center) the controller will use the set-point to activate the on/off flow of CO2 gas automatically.

When used with the Harvard Apparatus series of heating/coolingmicro-incubators, this controller provides a complete pH/tempera-ture controlled environment, ideal for long term cell or tissue stud-ies. The pH controller has a 4-digit LCD display capable of displayingthe full pH range from 0 to 14 pH. The microminiature combinationglass pH electrode that is supplied with the system is ideal for appli-cation involving placement of the pH electrode in a small area such asin our line of temperature controlled micro incubators, see pagesM96 to M98. The system is also compatible with any standard type of combination pH electrode.

A BNC connector on the rear of the unit provides a pH output (0 to 5 V) signal. This connection can be used to continuously log the pHvalues to a chart recorder, data acquisition system or other recordingdevice. The unit is powered by 12 VDC universal power supply forbenchtop use. The pH controller is shipped with: controller, powersupply, AC/DC adaptor, combination pH electrode and quick disconnect fittings matching front panel connectors for input and output hoses.

SpecificationsRange 0 to 14 pH

Resolution 0.01 pH

Accuracy ±0.02 pH

Input Resistance 1012 Ω

Calibration:Offset ±2 pH through offset trimmerSlope 80 to 110% through slope trimmer

CO2 Flowmeter 2 L per min.

Gas Input/Output Connectors Quick disconnect fittings

pH Analog Output BNC connector 4-20 mA, isolated

Electrode Response Time ~0 sec

Electrode Slope 55 mV/pH in pH range of 1 to 14

Electrode Impedance 200 MΩ

Electrode Stability 0.05 pH/day

Electrode Dimensions, L x D 1.5 x 0.03 in (38.1 x 0.75 mm)

System Power 12 VDC (Universal 90/240 VAC, 50/60 Hz adapter)

Dimensions, H x W x D 12.2 x 15.2 x 12.7 cm (4.8 x 6 x 5 in)

Weight 0.8 kg (1.75 lb)

Catalog No. $ ProductBS4 70-2116 1,119.00 Harvard Apparatus CO2 Gas/pH Controller

BS4 69-0494 424.00 Replacement pH Electrode, see page M23

BS4 65-0105 12.00 Input/Output Connector Fitting (5121-065), pkg. of 5

BS4 72-1015 11.00 Tygon Tubing (R-3603), 1/8 x 1/16 in (OD x ID)

For other compatible pH Electrodes,see pages M23 to M26.

• Precise pH level controlof liquid biological solutions using CO2

gas flow

• CO2 flow regulation from0 to 2 liters per minute

• Microminiature combination pH glasselectrode included withsystem, see page M23

• Easy setup

• Analog recorder output

• 12 V DC powered





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SpecificationsO2/CO2 /N2 Inlet Gas Supply Connectors:

1/4 in hose barb

Outlet Gas Stream Connectors:

Plastic quick disconnects for

1/16 in and 1/8 in ID tubing

Independent Channel Flow Control:Model SL2CO 0.5 to 10 mL/minModel SH2CO 0.1 to 5 L/min

O2 Range 0.1% to 99.9%

CO2 Range 0.1% to 20.0%

Accuracy ± 5% of reading; 2% full scale

O2 Sensor Fuel Cell

CO2 Sensor Infrared

Serial Comm. RS-232/RS485

O2 Analog Output 0 to 100 mV DC linear

CO2 Analog Output 0 to 1 V DC linear

Dimensions, H x W x D 36.1 x 23.6 x 33.0 cm(14.2 x 9.3 x 13.0 in)

Power 12 V DC

Weight 4.54 kg (10 lbs)

Catalog No. $ ProductBS4 64-0382 8,995.00 Oxystreamer - Dual Stream Oxygen and Carbon

Dioxide Controller 0 to 5 L/min

BS4 64-0383 8,995.00 Oxystreamer - Dual Stream Oxygen and CarbonDioxide Controller 0 to 10 mL/min

• Provides two identical streams of any mix of O2

and CO2 gas

• Full range real-time control of both gases

• Simultaneous control of both gas and liquid phases, useful for live cell microscopy

• Easily switch from one gas concentration to another

• Eliminates the inflexibility and high cost of premixed gas, and inaccuracies due to gas stratification in premixed gas tanks

• Vertical footprint saves bench space

The Oxystreamer® has two output streams can be used to simultane-ously condition the atmosphere in a microscope stage mount cellculture incubators/chambers and the dissolved oxygen and carbondioxide levels in media or perfusate. The following is a list of themajor benefits derived from replacing pre-mixed gas cylinders:

1. Precise, variable, selection of oxygen and carbon dioxide gasconcentrations (ratios)

2. Substantial cost savings by using standard, low cost cylinders ofoxygen, carbon dioxide and nitrogen gas

3. Eliminates the stratification induced concentration errors that areinherence in premixed gas cylinders

4. By independently monitoring and controlling oxygen and carbondioxide concentrations of the streams, the OxyStreamer® can monitor and proportionally mix (blend) oxygen, carbon dioxideand nitrogen gases into any concentration combination in real time.

The OxyStreamer® models are easy to use. After calibrating the oxygen and carbon dioxide sensors, the desired concentrations aresimply entered into their respective controllers using the digital display and up/down control buttons. The closed-loop dynamic gascontrol system will then automatically monitor and adjust the outputstream of gas to the pre-programmed gas ratios in the controllers.This technique will maintain the output mixed gas concentration towithin 0.2% of the selected set-point levels.

The flow rate for each stream is independently controlled for gas efficiency and to provide a method for setting a reproducible rate atwhich the gas is delivered to the cell culture chamber and/or diffused into the cell culture media or perfusate. The inlet ports forthe gases on the back of the unit are 1/4 inch hose barb fittings andwill take standard 1/4 inch ID tubing. Output stream ports are quick-disconnect fittings that use 1/16 inch or 1/8 inch ID tubing.

Options include a Windows® based software package that providestrend plotting, data logging, and remote operation via RS-232 connection to your PC. Multiple Oxystreamer® models can be daisychained via optional RS-485 interface.

Oxystreamer®NEW






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BSC-PC(Prechamber)The BSC-PC chamberallows several (10 to12) brain slices to bestored in vitro forseveral hours whilemaintaining excellentviability. A mixture of95% O2 /5% CO2 isbubbled through

physiological saline. This initiates a circular flow and oxy-gen enriched saline which continuously permeates theslices. Slices remain viable for hours while awaiting trans-fer to an examining chamber. Gas flow pressure should notexceed 5 p.s.i.

BSC-BU (Base Unit)The function of theBSC-BU Base Unit(catalog number BS465-0073) is to warmthe superfusing solu-tion. The BSC-BUconsists of a doublewalled cylinder

which is usually filled with distilled water and encloses anichrome heating element. The cavity also contains twoseparate sets of PVC tubing which can transport one ortwo superfusing solutions to the slice chamber. The baseunit also holds an aerator used to humidify vapor whenthe Haas Top is employed.

The BSC-BU is mounted on legs which suspend the heat-ing cylinder. This prevents the thermal expansion of theheating cylinder from moving the top unit and slice duringtemperature changes. The BSC-BU is specifically designedto accept both the BSC-HT and the BSC-ZT top units; thismakes for economy and versatility.

BSC-PC Prechamber

BS4 65-0608 Insert

Brain/Tissue Slice Chamber SystemBSC-BU Base Unit with BSC-HTHaas Top (interface chamber) andTC-202A Temperature Controller

BSC-BU Base Unit

This unique and versatileBrain/Tissue Slice Chamber Systemoffers the investigator two in vitromethods of studying a thin slice of tissue, which is bathed by perfusates ofinterest. The BSC-HT (Haas Top) is aninterface-type chamber in which onecut surface of the slice is wetted by aperfusing solution while the other surface is kept moist by a humidifiedgas mixture. The BSC-ZT (Zbicz Top) isa chamber in which solutions flowtransversely to fully submerged slices.

Each top unit allows for rapid changeover of fluids which are being investigated and both provide for excellent access to the slices for electrophysiological recording or other observations.

No Electrical InterferenceA low noise temperature controller is available (TC-202A) that utilizes wellfiltered DC heating current. This allows even the most sensitive electrophysiologic recordings such as single channel patch clamping to bemade from the slices without electrical interference.

ConvenientTemperature regulated perfusion lines are built in for rapid changeover ofsuperfusing fluids without the need of an external temperature regulatedperfusate supply tank.

Designed by ExpertsOver twenty years ago, Drs. Haas and Zbicz, two leading electrophysiologists, designed these chambers for use in their own research.Design improvements by Medical Systems over the next few decades havebeen enjoyed by electrophysiology labs worldwide.

ComponentsAll of the components must be purchased separately. See ordering information on page M148.

SpecificationsDimensions, L x W x H 114.6 x 139.45 x 111.13 mm

(4.511 x 5.490 x 4.375 in)

Weight 618 g (1 lb 5.8 oz)

Catalog No. $ ProductBS4 65-0073 730.00 BSC-BU (Base Unit)

SpecificationsDimensions, L x W x H 112.65 x 48.97 x 95.43 mm

(4.435 x 1.928 x 3.757 in)

Weight 362.87 g (12.80 oz)

Catalog No. $ ProductBS4 65-0076 395.00 BSC-PC Prechamber (for holding slices)

BS4 65-0608 93.00 Insert for BSC-PC Prechamber

• Maintains brain slicesfor 10 hours or moreon the bench-top

• Modular designaccommodates sub-mersible or interfacechambers

• 20 years of use inleading labs

• Sensitive recordingswithout electrical interference

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BSC-ZT (Zbicz Top)The BS4 65-0074 BSC-ZT ZbiczTop is a submersion type chamber in which tissue slices aresupported on a stiff nylon mesh.The perfusate flows transverselyacross both cut surfaces. Thesteady flow of warmed mediaacross the slightly submerged cutsurfaces of the slice is sufficient tokeep many tissues alive and

responsive for 10 or more hours. The fluid level is set to 1 mm above themesh by a dam at the end of the chamber. The chamber is 6 mm wide andthe trough is 24 mm long; this provides adequate room for slices andinstrumentation, yet minimizes the well volume to optimize drug changeover times. Fluid overflowing the dam is drained via a vacuum line towaste.Advantages

1. Slices remain viable for 10 hours or more.

2. The slices are mechanically stable. They are placed on a specialribbed nylon mesh and may be held in place by 3 to 4 stimulatingelectrodes or a plastic insert. This stability is attributed to the stiffnylon mesh which does not rise or sag with changes in fluid flow,therefore, moderate changes in the transverse laminar flow canbe well tolerated during intracellular and extracellular recordings.These features allow for switching of solutions with minimal riskof tissue movement and loss of neurons while recording.

3. The low volume of the chamber cavity and the relatively fast flowrate allows rapid exchange of solution (1 to 2 minutes) whichfacilitates drug application studies.

4. Slices remain moist and viable, because they are wetted on bothsides and do not dry out due to surrounding air currents.

5. The mesh surface holding the slice is semipermanent and can becleaned easily. The BSC-ZT comes with extra mesh which can beinstalled easily when necessary.

6. Because the fluid is shallow (400 to 600 microns) the electrodecapacitance remains low; an important consideration in singlemicroelectrode voltage and patch clamping.

7. Bubble formation is minimal. Bubbles, which form in the heatingunit, escape by the path formed at the entrance of the trough.Movement of the slice will not result should bubbles inadvertently form under the mesh. The flow of solution over theslice’s top surface will maintain tissue viability.

8. The solution temperature at the slice can be regulated preciselydue to the close proximity of a (optional) thermistor to the slice.

9. The slice can be transilluminated.

Brain/Tissue Slice Chamber SystemBSC-HT (Haas Top)The BS4 65-0075 BSC-HT HaasTop is an interface-type chamber2

utilizing semi-submersion princi-ples. It provides for rapidexchange of superfusing fluids,and excellent stability for intracel-lular recording. Slices rest onnylon mesh below which a thinsheet of the perfusate of interestflows. The bottom side of the slice

is wetted as capillary action saturates the mesh. Slices are oxygenated bya warmed and humidified mixture of 95% O2 /5% CO2 which flows acrossthe top surface of the slice. The BSC-HT has been designed such that, ifdesired, one can study the effects of two drugs simultaneously. One drugflows down the left portion of the chamber and the second down the right.

Laboratory results confirm that the semi-submersion top design has agood history of proven results:

• Maintains a stable intracellular membrane potential of morethan 60 mV.

• Facilitates action potentials with overshoots close to 100 mVin amplitude, duration of less than 2 msec at 32°C, whenused with hippocampal pyramidal and granular cells.

• Maintenance of the above potentials is possible for more than 1 hour undisturbed by changes in perfusion fluids.

• Preparation slices remain viable for up to 12 hours.

• Using flow rates of 1 ml/min, will completely exchange the chamber fluid in less than 1 minute.

BSC-HT (Haas Top) BSC-ZT (Zbicz Top)

SpecificationsDimensions, L x W x H 111.13 x 85.41 x 23.80 mm (4.375 x 3.365 x 0.937 in)

Weight 130.41 g (4.60 oz)

Catalog No. $ ProductBS4 65-0075 510.00 BSC-HT (Haas Top)

Harvard Apparatus nowowns Clark Electromedical. See pages M47 to M49 for the industry standardcapillary glass.

SpecificationsDimensions, L x W x H 98.55 x 101.60 x 28.19 mm (3.880 x 4.000 x 1.110 in)

Weight 124.74 g (4.40 oz)

Catalog No. $ ProductBS4 65-0074 510.00 BSC-ZT (Zbicz Top)

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Perfusion ControllersBrain/Tissue Slice Chamber System

Temperature ControllerThe matching temperature controller for the brain slice system is theTC-202A operating in its resistive heater (monopolar) mode (catalognumber BS4 65-0045), see page M125 for details. The BSC-T3(Thermistor), included with TC-202A, is designed to fit securely in theBSC-HT or the BSC-ZT. It is inserted downstream of the slice and isdesigned not to impede fluid flow across the slice. One design — onethermistor — is common to both tops.

Catalog No. $ ProductBS4 65-0045 2,305.00 TC-202A Biopolar Temperature Controller

References:

1. Zbicz, K.L. & Weight, F.F. “Transient Voltage and Calcium Dependent Current...”J. Neurophys 53,1038-1058 (1985).

2. Haas, H.L. et al “A Simple Perfusion Chamber for the Study of Nervous Tissue Slices inVitro” J. Neurosci Meth 1, 323-325 (1979).

Ordering InformationAll components are ordered separately. Most researchers order theBSC-BU Base unit with either the Haas or Zbicz top, depending onindividual research. The pre-chamber is a very effective accessoryfor slice preservation, but is not necessary for system operation.We recommend ordering the BSC-ACC-Kit (not included with anypreviously described components) because it contains valuablereplacement parts such as screws, fittings, and mesh.

BSC-BU Base Unit with BSC-HTHaas Top (Interface Chamber) and

TC-202A Temperature Controller

Catalog No. $ Model ProductSystem Components

BS4 65-0073 759.00 BSC-BU Base Unit Including Tubing, HeatingElement and Electrical Terminals

BS4 65-0074 510.00 BSC-ZT Zbicz Top Including Nylon Mesh

BS4 65-0075 510.00 BSC-HT Haas Top Including 2 Slice Holders

BS4 65-0076 395.00 BSC-PC Prechamber for Holding Slices

Brain/Tissue Slice Chamber Accessories

BS4 65-0098 162.00 BSC-T2 Replacement T2 Thermistor for TC-102

BS4 65-0057 327.00 BSC-T3 Thermistor (Bath) for Use with BrainSlice Chamber

BS4 65-0078 33.00 BSC-AIR Aeration Element Replacement, Set of 2

BS4 65-0079 43.00 BSC-ORING Base Unit (BSC-BU) ReplacementGasket Set

BS4 65-0080 26.00 BSC-HE Base Unit Heating Element Replacement

BS4 65-0081 25.00 BSC-TUBE Base Unit (BSC-BU) Tubing SetReplacement, Tygon .031 x .093 (ID x OD)

BS4 65-0082 41.00 BSC-G10 Haas Top (BSC-HT) Tissue InsertReplacement, Set of 2

BS4 65-0083 55.00 BSC-HTC Haas Top (BSC-HT) Plastic CoverReplacement

BS4 65-0084 45.00 BSC-HT-AG Haas Top (BSC-HT) Ground WireReplacement

BS4 65-0086 82.00 BSC-ZTC Zbicz Top (BSC-ZT) Plastic InsertReplacement

BS4 65-0087 22.00 BSC-PC-VAL Prechamber (BSC-PC) ValveReplacement

BS4 65-0088 71.00 BSC-ACC-KIT Base Unit, Haas & Zbicz Tops AccessoryKit (Replacement Screws, Fittings and Mesh)

BS4 65-0091 78.00 BSC-CABLE Base Unit (BSC-BU)/TemperatureController (TC-102) Replacement Power Cable

BS4 65-0090 37.00 BSC-MESH Haas Top (BSC-HT) Zbicz Top (BSC-ZT)Replacement Mesh 300 µm grid, 5 x 5 in, pkg. of 4

BS4 65-0104 68.00 – Output Cable, 6-Pin to Banana for BS4 65-0045 TC-202A or BS4 65-0073BSC-BU

BS4 65-0100 89.00 – Output Cable, 6-Pin to 1 mm for BS4 65-0045 TC-202A, BS4 65-0040LU-CB1 and BS4 65-0041 LU-CPC-CEH

For Monopolar Temperature Controller,see page M125.

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