For research use only. Not for use in diagnostic procedures.

Quantification of 360+ Lipids, 40+ Small Molecules with Q ExactiveTM Mass Spectrometers

AbsoluteIDQ® p400 HR KitBroad Lipid and Metabolic Profi ling

AbsoluteIDQ® p400 HR Kit® p400 HR Kit®

Ready-to-Use

Superior Data Quality

Simple & Guided Workfl ow

High Throughput & Minimal Sample

Volume

KIT

Performance confi rmed by ring tria

l

Thompson et al., Anal Chem 2019

Instrument Platforms* (UHPLC Required)

Metabolite Coverage

Kit Components

KIT

* not suited for nano or micro fl ow systems

The AbsoluteIDQ® p400 HR Kit is a complete solution for the quantifi cation of 365 lipids and 43 small molecules with high resolution, accurate mass (HRAM) OrbitrapTM mass spectrometers. The broad analyte coverage of the kit allows researchers to obtain comprehensive information about the biochemical phenotype of an organism, and about a multitude of pathophysiological processes. The kit generates high throughput results with confi dence. The AbsoluteIDQ® p400 HR Kit is based on Biocrates’ proprietary technology that has contributed to more than 800 scientifi c publications.

Key Benefi tsBroad Lipid and Metabolic Profi ling – Simple, Fast, Quantitative, Reproducible

Key FeaturesUp to 408 Endogenous Metabolites from 11 Metabolite Classes

Ready-to-Use• Implemented within one day• All in one box: no method or reagent

development required• For a broadly established standard

instrument base

High Throughput & Minimal Sample Volume• Up to 240 samples per week and instrument• 10 µL needed for most biofl uids

(validated for human EDTA plasma)

Simple, Guided, Automated Workfl ow• Workfl ow manager guided procedure

for enhanced process safety• Automated signal identifi cation, quantifi cation,

and technical validation

Superior Data Quality• Quantitative results• Proven analytical performance:

excellent accuracy and precision• Enhanced Inter-laboratory, inter-instrument, and

longitudinal reproducibility

Small Molecules• Amino acids (21)• Biogenic amines (21)• Hexoses (1)

Polar Lipids• Phosphatidylcholines (172)• Lysophosphatidylcholines (24)• Sphingomyelins (31)• Ceramides (9)

Neutral Lipids• Acylcarnitines (55)• Cholesteryl esters (14)• Diglycerides (18)• Triglycerides (42)

Reagents & Consumables• Patented 96-well fi lter plates• Internal & calibration standards• Quality controls• Testmix

Methods & Protocols• Sample preparation protocol• Instrument-specifi c acquisition &

quantifi cation methods• System suitability test

Workfl ow Manager MetIDQ™• Process guidance• Automated technical validation• Basic statistics (optional)

• Q Exactive™ Focus MS • Q Exactive™ MS • Q Exactive™ Plus MS • Q Exactive™ HF MS

0

20

40

60

80

100

120

140

160

Accu

racy

(%),

n=3

NIST SRM 1950

Plate LayoutEasy to use 96-well format, for up to 80 samples

Blank; no sample, no ISTD

Zero sample; no sample, only ISTD

Quality controls (QC) in low, medium, high concentration

Samples

7 point calibrator standardsfor LC-MS part

A1 2 3 4 5 6 7 8 9 10 11 12

BCDEFGH

Internal Standard (ISTD)

Blank; no sample, no ISTD

AbsoluteIDQ® p400 HR Kit plate

Zero sample; no sample, only ISTD

Samples

Internal standard (ISTD)

7 point calibrator standards for LC-MS part

Quality controls (QC) in low, medium, high concentration

Accuracy Range 90-114%

The AbsoluteIDQ® p400 HR Kit provides excellent accuracy (against reference material, see fi gure right). High inter-laboratory and inter-instrument accuracy and precision have been proven in a multicenter study including 3 laboratories and 6 instruments (Koal et al, Metabolomics 2017; Poster 56).

The kit is suitable for use in translational and longitudinal studies, as well as large-scale and multi-center research endeavours.

Superior Data QualityAccuracy, Precision & Reproducibility

Accuracy vs. NIST Standard

Acc

urac

y (%

), n=

3

Simple, Guided, Automated Workfl owFully Validated Workfl ows for High Throughput and Confi dence in Results

15 min1 h

Sample Registration• Register samples• Defi ne plate layout• Generate worklist

Sample Preparation• Perform system suitability test• Prepare plates (loading of samples,

derivatization & extraction)

Sample Measurement• Run samples with LC- and FIA-MS• Instrument-specifi c acquisition

methods are provided

Technical Validation• Perform automated sensitivity and

accuracy check• Inter-plate monitoring of QC results

and precision

Statistics• Perform tests with MetIDQ™ StatPack Module• Use statistical programs of your choice• On demand data analysis by Biocrates

Quantifi cation & Data Processing• Automated signal identifi cation

and quantifi cation (FIA data)• Merge LC and FIA data

Data Evaluation & Export• Normalize data (optional)• Export results in various fi le formats

Biological Interpretation• HMDB• Consult Biocrates

for support

Workflow Manager MetIDQ™Total: 36 h for up to 80 samples

optional

4 h

30 min 15 min30 h

Phenotype

Hexoses• Aberrant glycolysis

• Insulin resistance

Cholesteryl Esters• Insulin resistance

• Aberrant cholesterol transport

Amino Acids• Energy homeostasis

• Insulin resistance

• Oxidative stress

Diglycerides• Oxidative stress

• Infl ammation

• Aberrant signal transduction

Triglycerides• Oxidative stress

• Insulin resistance

Lysophosphatidylcholines• Infl ammation

• Oxidative stress

Sphingomyelins• Neurodegeneration

• Oxidative stress

Biogenic Amines• Neurotransmitter deregulation

• Aberrant cell cycle control

Acylcarnitines• Mitochondrial dysfunction

• Aberrant fatty acid oxidation

Phosphatidylcholines• Deregulated growth/proliferation

• Infl ammation

Ceramides• Endoplasmic reticulum stress

• Aberrant cellular signaling

How to Get StartedMultiple Options for Obtaining Results

Your Laboratory Collaborating Laboratory Metabolic Phenotyping Services

You have the right instrumentation to run the kit?

Please contact us for a detailed list of requirements and a quote.

You want to run the kit in a collaborating laboratory?

Contact us, and we will help you to fi nd the right partner.

You want us to measure your samples?

Biocrates can perform the full kit workfl ow, as well as additional services, with short turnaround times. Contact us for further information.

Broad Metabolite CoverageRelevance in Key Pathophysiological Processes

The AbsoluteIDQ® p400 HR Kit provides broad information on the status of biological systems, from structural information and cell signaling, to bioenergetics. Selected pathophysiological processes represented by the metabolite classes included in the AbsoluteIDQ® p400 HR Kit are shown below.

Product IP protected kit: PCT/EP2006/006328, PCT/EP2006/006327, EP20080015225

For research use only. Not for use in diagnostic procedures.

Document Nr. 35 026, V02-2019Photos: Biocrates, Fotolia, Depositphoto

Related ProductsAbsoluteIDQ® p400 HR Kit

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AbsoluteIDQ® p400 HR Kit Setup Box

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