Breeding potato with BreeDB

10
Breeding Potato with BreeDB 31 January 2013, Richard Finkers (@rfinkers) Heleen de Weerd, Benoit Carreres, et al.

description

BreeDB is a breeding database and contains an extensive breeding toolbox. BreeDB is developed by Wageningen UR Plant Breeding (https://www.wageningenur.nl/en/show/BreeDB.htm)

Transcript of Breeding potato with BreeDB

Page 1: Breeding potato with BreeDB

Breeding Potato with BreeDB

31 January 2013, Richard Finkers (@rfinkers)

Heleen de Weerd, Benoit Carreres, et al.

Page 2: Breeding potato with BreeDB

Outline

Project flow

Developed tools

Where are we now

Page 3: Breeding potato with BreeDB

Genotyping•Genotyping-by-sequencing

•SNP arrays

Dosage information•fitTetra

Optional: Population Structure correction

LD analysis Marker selection

Future: in sillico

cultivar design

BreeDB Flowchart

Field trial•Observations•~omics analysis

Estimate means

Page 4: Breeding potato with BreeDB

Allele dosage estimation

Example: SNP genotyping array (20.000 loci)

Calculate Allele dosage (R: fitTetra)

Prefer: haplotype reconstruction

doi:10.1186/1471-2105-12-172

Page 5: Breeding potato with BreeDB

Population structure

Software: structure

Segments: old varieties/ American varieties / Fresh consumption / Starch

Page 6: Breeding potato with BreeDB

Pedigree

Potato pedigree

Phenotypic observations

Page 7: Breeding potato with BreeDB

Association analysis

Page 8: Breeding potato with BreeDB

Predictive models

Models based on 5-10 markers

Page 9: Breeding potato with BreeDB

Genotyping•Genotyping-by-sequencing

•SNP arrays

Dosage information•fitTetra

Optional: Population Structure correction

LD analysis Marker selection

Future: in sillico

cultivar design

BreeDB Flowchart

Field trial•Observations•~omics analysis

Estimate means

Page 10: Breeding potato with BreeDB

Questions?