Blood processing serum and plasma

Blood sample processing 1.Blood serum preparation- Blood serum is blood plasma without the clotting factors. As serum has fewer proteins it is clear than plasma. Procedure- The collected blood is transferred the collected blood in to the eppendorf (centrifuge) tube. The transferred blood should be approximately 2.5 times the volume needed for use. (For eg 5ml blood is required for 2ml serum). The resultant tube is to be kept for incubation in an upright position for a period of 30-45min to allow clotting. Then it is centrifuged using a cold centrifuge for a period of 15min at 1000-2000RPM. Using a clean pipette aspirate the supernatant serum and pour into another eppendorf tube. Inspect for the turbidity. If turbidity is seen, then it is centrifuged again. The serum samples should be stored in cold freezer (-80 o C). 2.Blood plasma preparation- Blood plasma is the liquid component of blood, in which the blood cells are suspended. Plasma is the supernatant fluid obtained when anti-coagulated blood has been centrifuged. Procedure- Transfer the collected blood into eppendorf tube containing an anticoagulant (1.8mgK 2 EDTA/ml of blood). The tube is inverted 10times to mix the blood and anticoagulant. The resultant mixed blood is centrifuged for 10min at 1000-2000RPM at room temperature. The supernatant clear plasma is aspirated into another eppendorf tube. Inspect for the turbidity. If turbidity is seen, then it is centrifuged again. The plasma samples should be stored in cold freezer (-80 o C).

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Transcript of Blood processing serum and plasma

Page 1: Blood processing serum and plasma

Blood sample processing

1.Blood serum preparation- Blood serum is blood plasma without the clotting factors.As serum has fewer proteins it is clear than plasma.Procedure- The collected blood is transferred the collected blood in to the eppendorf (centrifuge)tube. The transferred blood should be approximately 2.5 times the volume needed for use. (Foreg 5ml blood is required for 2ml serum). The resultant tube is to be kept for incubation in anupright position for a period of 30-45min to allow clotting. Then it is centrifuged using a coldcentrifuge for a period of 15min at 1000-2000RPM. Using a clean pipette aspirate the supernatantserum and pour into another eppendorf tube. Inspect for the turbidity. If turbidity is seen, then itis centrifuged again. The serum samples should be stored in cold freezer (-80oC).

2.Blood plasma preparation- Blood plasma is the liquid component of blood, in which theblood cells are suspended. Plasma is the supernatant fluid obtained when anti-coagulated bloodhas been centrifuged.Procedure- Transfer the collected blood into eppendorf tube containing an anticoagulant(1.8mgK2EDTA/ml of blood). The tube is inverted 10times to mix the blood and anticoagulant.The resultant mixed blood is centrifuged for 10min at 1000-2000RPM at room temperature. Thesupernatant clear plasma is aspirated into another eppendorf tube. Inspect for the turbidity. Ifturbidity is seen, then it is centrifuged again. The plasma samples should be stored in cold freezer(-80oC).