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Transcript of Blood
I. Introduction
In blood transfusion (moves to decant), the human whose get blood
is called receivers (recipient) and blood giver is called donor. Blood
transfusion event to recipient very related to the blood group kind. Blood
group kind follows system ABO determined by kind agglutinogen in
erythrocyte. There two kinds agglutinogen a, and agglutinogen b.
Somebody blood can contain one of kind agglutinogen, or the two, and or
doesn’t contain agglutinogen. Blood clotting reaction in blood group test
happens because the reaction between agglutinin from plasma with
agglutinogen from erythrocyte. In blood found containing sum of
hemoglobin blood. The hemoglobin contain can it to knowable with
measures with Talquit paper and also Sahli Hbmeter.
Besides the Hb in blood it can was found a thrombocyte when will
broken will take outside thromboplastine that will spur on formation
thrombin from prothrombin. And thrombin will be blood coagulation
mobile enzyme because will can to change fibrinogen be threads fibrin
that will close wound. Blood coagulation speed is influenced by several
factors among others factor has countered hemophilia, vitamin K and
pregnancy ion Ca2+.
Blood can go around to entire bodies pass blood vessel be caused
pressure existence in ventricle space. Ventricle pressure distinguishable
pressure systole and pressure diastole. This ventricle pressure measurable
by means of that called sphygmomanometer.
Based on blood dependability with various this life importance
necessary presumably very valuable when we can detect principles and
the measurement manners so that can useful for life furthermore
II. Problems
1. How does manner measures blood pressure and blood pressure
variation in each student?
2. How does blood group testing manner and blood group variation in
each student?
3. How does degree of measurement manner hemoglobin and degree of
variation hemoglobin in each student?
4. How does to measurement of blood coagulation time and blood
coagulation time variation in each student?
III. Objectives
1. To detect blood pressure measurement manner and blood pressure
variation in each student.
2. To detect blood group testing manner and blood group variation in
each student.
3. To detect degree measurement manner hemoglobin and degree
variation hemoglobin in each student.
4. To detect manner measures time coagula blood and blood
coagula/coagulation time variation in each student.
IV. Literature
The main transportation in human is blood. In the body, blood
circulate with circulatory organ which are heart and blood vessels. Beside
blood circulation, there is also limfe circulation that circulate with limfe
vessels. Blood consist of leukocytes, erythrocyte, and platelets.
Blood has three main functions: transport, protection and regulation.
Blood is classified as a connective tissue and consists of two main
components:
1. Plasma, which is a clear extracellular fluid
2. Formed elements, which are made up of the blood cells and
platelets
The formed elements are so named because they are enclosed in a
plasma membrane and have a definite structure and shape. All formed
elements are cells except for the platelets, which tiny fragments of bone
marrow cells(www.virtualmedicalcentre.com)
Formed elements are:
Erythrocytes, also known as red blood cells (RBCs)
Leukocytes, also known as white blood cells (WBCs)
Platelets
Blood grouping as known as blood ABO grouping. This blood
grouping is invented by Karl Landsteiner. Blood grouping is based on
the content of aglutinin / antibody in the blood. In our blood, there are
aglutinogen(antigen) in erythrocytes and aglutinin(antibody) in
plasma. Grouping the blood is started from blood clotting when the
erythrocytes mixed up with other blood serum(R.Gunawan, 2007:125).
Blood grouping based on the antigen and antibody:
Blood group AB individuals have both A and B antigens on
the surface of their RBCs, and their blood plasma does not contain any
antibodies against either A or B antigen. Therefore, an individual with
type AB blood can receive blood from any group (with AB being
preferable), but can donate blood only to another type AB individual.
Blood group A individuals have the A antigen on the surface
of their RBCs, and blood serum containing IgM antibodies against the
B antigen. Therefore, a group A individual can receive blood only
from individuals of groups A or O (with A being preferable), and can
donate blood to individuals with type A or AB.
Blood group B individuals have the B antigen on the surface
of their RBCs, and blood serum containing IgM antibodies against the
A antigen. Therefore, a group B individual can receive blood only
from individuals of groups B or O (with B being preferable), and can
donate blood to individuals with type B or AB.
Blood group O (or blood group zero in some countries)
individuals do not have either A or B antigens on the surface of their
RBCs, but their blood serum contains IgM anti-A and anti-B
antibodies against the A and B blood group antigens. Therefore, a
group O individual can receive blood only from a group O individual,
but can donate blood to individuals of any ABO blood group (i.e., A,
B, O or AB). If anyone needs a blood transfusion in an emergency, and
if the time taken to process the recipient's blood would cause a
detrimental delay, O Negative blood can be
issued(http://en.wikipedia.org/wiki/Blood_type)
Blood pressure (BP) is the pressure exerted by circulating
blood upon the walls of blood vessels, and is one of the principal vital
signs. When used without further specification, "blood pressure"
usually refers to the arterial pressure of the systemic circulation.
During each heartbeat, BP varies between a maximum (systolic) and a
minimum (diastolic) pressure. The mean BP, due to pumping by the
heart and resistance to flow in blood vessels, decreases as the
circulating blood moves away from the heart through arteries. Blood
pressure drops most rapidly along the small arteries and arterioles, and
continues to decrease as the blood moves through the capillaries and
back to the heart through veins. Gravity, valves in veins, and pumping
from contraction of skeletal muscles, are some other influences on BP
at various places in the body
( http://en.wikipedia.org/wiki/Blood_pressure)
To check the Hb(Hemoglobin), we can use Talquist method.
It’s indicated by matching the colour of the blood in filters paper with
the indicator in Talquist book. Beside that, the another way to check
the hemoglobin with Sahli method. Sahli method is one way of
visually determining hemoglobin. Blood was diluted with HCl solution
so that the hemoglobin turns into acid hematin. To be able to
determine the levels of hemoglobin solution made by diluting the
mixture with distilled water until the color the same color as the
standard glass rod.
V. Hypothesis
1. Measure blood pressure is using sphygmanometer. How to
measure is :
Examining installing rubber wrapped in a cloth bag (cuff)
on the upper arm
Stethoscope placed on the inside of the elbow crease.
Rubber bag was then developed by pumping air into it.
Rubber bag that dilate blood vessels will press the arm
(brachial artery) so that blood flow is suspended
The air then removed slowly by turning the air cap.
When the air pressure inside the rubber bag is lowered,
there are two things to watch out for inspectors. First, the
needle pressure, the second sound pulse of the blood
vessels of the arm that delivered through a stethoscope.
When you hear the beats for the first time, the value of the
needle pressure is systolic pressure value.
Along with the continued drop in air pressure, pulse sound
is heard through the stethoscope will dissapear. The value
indicated by the needle when the sound pressure is
dissapeared is called diastolic pressure pulse.
There are two variation of blood pressure there are systolic
pressure and diastolic pressure.
2. ABO blood group is checked by taking 2 drops of capillary blood
placed on a seperate slide. The first drop adding by 1 drop of
serum containing anti – A, and second drop add by 1 drop of
serum anti – B. Adding of 0,9 % NaCl solution, then do the
dilution. What to see is a reaction of agglutination (clumping
shaped spots of dark red or blackish red), whch occurs between
aglutinin and aglutinogen accordingly. It’s same with Rh test.
Serum is added containing anti – D (anti – Rh), so if it looks the
results of agglutination is Rh positive, and the opposite.
The variation of blood type are A, B, AB, and O
3. There are seveal ways to measure the hemoglobin that contain in
blood, mostly carried out by automatic machines to make some
test of the blood. The other way is with talquist method. This
method is matching the blood colour with the colour in talquist
book.
The variation of hemoglobin in every student is 12 – 16 gr/dl.
4. To find the coagulation time, we can use this ways :
Dropped the blood sample (1 drop) in object glass and after
finish dropping, count the time with stopwatch
Every 30 seconds, pin the blood with needle.
When the last pin there are fibrin thread that sticking in the
tip of needle, it means that occurs coagulation, then note
the time with stopwatch.
For normal coagulation time, it’s 15 seconds – 2 minutes.
Coagulation time is caused bby many factors.
VI. Tools and Materials
Tools : Materials :
1. Sphymanometer 1. Alcohol
2. Stethoscope 2. Filters paper
3. Blood lancet 3. Aquades
4. Needle 4. Anti A
5. Colorimeter Hb and Hb Sahli 5. Anti B
6. Object Glass 6. Cotton
7. Stop watch
VII. Working Procedures
A. Blood Pressure Determinations
1. Student left arm at bandages sphygmanometer
2. Feel on the bottom arm bandages and look for the artery blood
vessel (arteria brachialis). After found to put stethoscoe head
above artery, this manner is called auskultatoir. Manner other with
feel artery and fell to beat the pulse is called palpatoir.
3. Air contents in bandages with pump slowly so that needle
manometer show scale 170 mmHg.
4. Release air in bandage slowly with twist pump casing valve, while
pay attention scale needle depreciation in manometer and ear
concentrates stethoscope.
5. When does pulse heard first time in stethoscope register number
that indicated needle manometer, because this show pressure
systole. Heartbeat continual will happen periodical and more
weaker and final lost. At the time of lost to return needle position
manometer, because this show pressure diastole.
B. Blood Group Determination
1. Clean fingertip middlestudent with cotton that wwetted with
alcohol 70%.
2. Jab blood lancet to fingertip miiddle student. Massage fingertip so
thet easy blood out, then drip secretor blood in object glass a and
drip also in object glass b.
3. When has blood been dribbled, clean fingertip with alcohol 70%
again, in object glass b.
4. Give serum driblet counters in a blood at glass a and serum
counters b in glass.
5. Stir blood drop with needle.
6. Perceive what happen blood clotting or not? Determine blood
based on explanation next :
When:
a. Blood at A is thickening at B not so belonging blood group A.
b. Blood at A is not thickening at B is thickening is belonging
blood group B.
c. Blood at A and at B thicken, so belong blood group AB.
d. Blood at A and at B not thicken belong blood group O.
C. Hemoglobin Degree Determination
1. Talquist Method
a. Drip blood in filter paper that provided.
b. Let during 30s.
c. After 30 s check off blood color in filter paper with calorimeter
Hb available in Talquist book and register the result in percent.
d. Result in percent can be change in unit mg% by as follows
%result x 22 =...........mg%
100
D. Coagulation Time
1. Clean fingertip student with cotton that wetted with alcohol 70%.
2. Jab blood lancet to fingertip middle student. Massage fingertip so
thet easy blood out, then drip object glass and moment begin to
dripnto animate stop watch.
3. Every 30 sec blood drop is lifted or at pull with needle end.
4. When in latest pricking found thread fibrin that cling at the end of
needle mean happen coagula, kill watch and register the result.
IX. Discussion
Hb Degrees
50%(11 mg%)60%(13,2mg%)70%(15,4mg%)80%(16,7mg%)
Based on the observation of XI IPA 2 students, we can find that most
of the students have 60 % degrees of Hb. It’s indicated by matching the
colour of the blood in filters paper with the indicator in Talquist book.
Based on the observation, we can find that most of students has O
blood type. The blood type are based on the antigen and antibody. Individuals that
have both A and B antigens, and their blood plasma does not contain any antibodies
against either A or B antigen can be classified as blood group AB. Individuals have
the A antigen, and blood serum containing antibodies against the B antigen can be
classified as blood group A. Individuals have the B antigen, produced antibodies
against the A antigen can be classified as blood group B. Individuals that do not have
either A or B antigens, but produced antibodies against the A and B blood group
antigens.
Blood type
ABABO
Based on the observation, we can find that most of students in XI IPA 2 has
coagulation time under 3 minutes. We can indicated by prick the needle into the
blood. If it found fibrin thread, it means coagulation has been working.
50 - 59 60 - 79 80 - 99 100 - 119 120 - 139 140 - 159
0
5
10
15
20
25
SystoleDiastole
Based on the observation, we can find that the variation of blood pressure of
the students is shown in the figure above. We can checked the blood pressure with
sphygmanometer and sthetoscope. The normal blood pressure is 120/80 mmHg.
Based on the graph, we can conclude that most of the student have normal blood
pressure.
The next thing to observe is sugar content. In normal condition, sugar content
in our blood is 38 – 132 mg/dl. Base on the observation table, we can conclude that
sugar content in student’s blood is normal. In normal condition,the content of
colesterol in our blood is 160 – 240 mg/dl. So, we can conclude that most of student
has normal colesterol content. In normal condition, the content of uric acid in our
blood is 3.0 – 6.0 mg/dl. So, we can conclude that most of the student has normal
content of uric acid. These 3 things, we can checked with a tool that can checked
Blood Pressure
Coagulation Time
3''< 3"> 3"
sugar, colesterol, and uric acid content simultaneously. If we found the unnormal
content, so we can found it’s –HI- or –LO-. It means that the tools cannot read the
measurement.
X. Conclusion
1. Blood pressure can be measured with sphgymanometer. We can
measure it by take the stethoscope right in the vein. Then, bandage the
arm with sphygmanometer and pump it until 170. After that, open the
valve until we hear the first pulse it called systole, and the last pulse is
called diastole. From the observation result, the most student has 120 –
139 systole, and 60 – 79 diastole.
2. Blood type variation can be measured by taking a few drops of blood
and mix them with serum anti A and anti B. By doing the observation,
we can conclude that 3 students of XI IPA 2 has blood type A, 7
students has blood type B, 3 students has blood type AB, and 19
students has blood type O.
3. Hb degrees can be measured by taking a drop of blood, then put it on
the filter paper. After that, match the colour with Talquist book. From
the observation result, we can find that 2 students has 50% (11 mg%)
of Hb, 19 students has 60% (13,2 mg%) of Hb, 8 students has 70%
(15,4 mg%) of Hb, and 1 student has 80% (16,7 mg%) of Hb.
4. Blood coagulation can be measued by put a few drops of blood in the
object glass, and after 30 seconds stir with needle. When there is a
blood lump in the tip of the needle, so it means that coagulation has
been working. Most of the students has coagulation time under 3
minutes.
XI. References
http://gurungeblog.wordpress.com/2008/10/31/sistem-transportasiperedaran-darah-pada-manusia/ (Saturday, Sept 3rd ; 14:50 WIB)
http://www.virtualmedicalcentre.com/anatomy.asp? sid=30&title=Blood-Function-and-Composition (Saturday, Sept 3rd; 14:50 WIB)
http://en.wikipedia.org/wiki/Blood_type ( Monday, August 29th ; 13:30 WIB)
Susilowarno Gunawan,dkk.2007.Biologi SMA/MA Kelas XI.Jakarta:Grasindo.
BLOOD
By :
Ni Wayan Diana C.
XI IPA 2/18
STELLA DUCE 1 SENIOR HIGH SCHOOL 2011/2012
Biology Laboratory
Stella Duce 1 Senior High School