Biotechnology Biology 122 Genes and Development. Red arrows are the cut sites; Green dots indicate...
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Transcript of Biotechnology Biology 122 Genes and Development. Red arrows are the cut sites; Green dots indicate...
Red arrows are the cut sites; Green dots indicate the center of the sequence
←Produces blunt ends
Restriction enzymes
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
b: © 2007, Illumina Inc. All rights reserved
Adapter
Adapter
Adapters
a. b.
Flow cell1 cm
DNA
DNA fragment
Dense primer lawnin flow cell
Sequencing by synthesis
Animation of Illumina sequencing
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
c. d.
Bridgeamplificationwith unlabeleddNTPs
Free endbinds toprimer
Fragmentsbecomedouble-stranded
Freeterminus
Attached
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
Attached
c. d. e.
Bridgeamplificationwith unlabeleddNTPs
Free endbinds toprimer
Fragmentsbecomedouble-stranded
Freeterminus
Denaturedouble-strandedmolecules
Attached
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
Attached Clusters
c. d. e. f.
35 cyclesof bridgeamplification
Bridgeamplificationwith unlabeleddNTPs
Free endbinds toprimer
Fragmentsbecomedouble-stranded
Freeterminus
Denaturedouble-strandedmolecules
Attached
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
A
A
T
TG
GC
C
g.
First round ofsynthesis withlabeled dNTPs
Transgenic mouse
Ventral view of a 14-day-old transgenic mouse, carrying a reporter gene that is active in the developing limb.
Stereomicroscopy Photo by Dr. Mohammad K. HajihosseiniUniversity of East Anglia, Norwich, U.K.Bioscapes 2009 photo contest