BIOTECHNOLOGY BIOENGINEERING -...

Volume 102, Number 4, March 1, 2009

FEATURES

vii Spotlight

Vintage Paper

977 Damage Mechanisms of Suspended Animal Cells in Agitated Bioreactors with and Without Bubble EntrainmentK.T. Kunas and E.T. Papoutsakis

Introduction by E.T. Papoutsakis

ARTICLES

Biocatalysis, Protein Engineering, and Nanobiotechnology

988 Expanding Substrate Specifi city of GT-B Fold Glycosyltransferase Via Domain Swapping and High-Throughput ScreeningS.-H. Park, H.-Y. Park, J.K. Sohng, H.C. Lee, K. Liou, Y.J. Yoon, and B.-G. Kim

Published online 29 September 2008

995 Biocatalytic Dechlorination of Trichloroethylene With Bio-Palladium in a Pilot-Scale Membrane ReactorT. Hennebel, H. Simoen, W. De Windt, M. Verloo, N. Boon, and W. Verstraete


1003 A Novel Aryl Acylamidase From Nocardia farcinica Hydrolyses PolyamideS. Heumann, A. Eberl, G. Fischer-Colbrie, H. Pobeheim, F. Kaufmann, D. Ribitsch, A. Cavaco-Paulo, and G.M. Guebitz


1012 Development of a Denaturation/Renaturation-Based Production Process for Ferritin NanoparticlesA. Jääskeläinen, T. Soukka, U. Lamminmäki, T. Korpimäki, and M. Virta


1025 Hydrogen/Deuterium Exchange Study of Subtilisin Carlsberg During Prolonged Exposure to Organic SolventsE. Fasoli, A. Ferrer, and G.L. Barletta


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Biofuels and Environmental Biotechnology

1033 An Optimized Microplate Assay System for Quantitative Evaluation of Plant Cell Wall-Degrading Enzyme Activity of Fungal Culture ExtractsB.C. King, M.K. Donnelly, G.C. Bergstrom, L.P. Walker, and D.M. Gibson


1045 High-Level Bacterial Cellulase Accumulation in Chloroplast-Transformed Tobacco Mediated by Downstream Box FusionsB.N. Gray, B.A. Ahner, and M.R. Hanson

Published online 3 October 2008

1055 Hydrogen Production by Photoautotrophic Sulfur-Deprived Chlamydomonas reinhardtii Pre-Grown and Incubated Under High LightI.V. Tolstygina, T.K. Antal, S.N. Kosourov, T.E. Krendeleva, A.B. Rubin, and A.A. Tsygankov


Bioprocess Engineering and Supporting Technologies

1062 Maintenance and Growth Requirements in the Metabolism of Debaryomyces hansenii Performing Xylose-to-Xylitol Bioconversion in Corncob Hemicellulose HydrolyzateB. Rivas, P. Torre, J.M. Domínguez, and A. Converti


1074 Production of Mouse Interleukin-12 Is Greater in Tobacco Hairy Roots Grown in a Mist Reactor Than in an Airlift ReactorC. Liu, M.J. Towler, G. Medrano, C.L. Cramer, and P.J. Weathers


1087 Engineering Cell Lines for Production of Replication Defective HSV-1 Gene Therapy VectorsK.G. Grant, D.M. Krisky, M.M. Ataai, and J.C. Glorioso III


1098 In Situ 2D Fluorometry and Chemometric Monitoring of Mammalian Cell CulturesA.P. Teixeira, C.A.M. Portugal, N. Carinhas, J.M.L. Dias, J.P. Crespo, P.M. Alves, M.J.T. Carrondo, and R. Oliveira


1107 A Clone Screening Method Using mRNA Levels to Determine Specifi c Productivity and Product Quality for Monoclonal AntibodiesC.J. Lee, G. Seth, J. Tsukuda, and R.W. Hamilton


1119 Evaluation of the Effect of Chronic Hydrodynamical Stresses on Cultures of Suspensed CHO-6E6 CellsR. Godoy-Silva, M. Mollet, and J.J. Chalmers


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Bioseparations and Downstream Processing

1131 Modeling Electrostatic Exclusion Effects During Ion Exchange Chromatography of Monoclonal Antibodies A.L. Zydney, C. Harinarayan, and R. van Reis


1141 Selective Antibody Precipitation Using Polyelectrolytes: A Novel Approach to the Purifi cation of Monoclonal AntibodiesP. McDonald, C. Victa, J.N. Carter-Franklin, and R. Fahrner


Cellular and Metabolic Engineering

1152 Mutated Polyadenylation Signals for Controlling Expression Levels of Multiple Genes in Mammalian CellsY. Yang, Mariati, S.C.L. Ho, and M.G.S. Yap


1161 Metabolic Flux Analysis of Shewanella spp. Reveals Evolutionary Robustness in Central Carbon MetabolismY.J. Tang, H.G. Martin, P.S. Dehal, A. Deutschbauer, X. Llora, A. Meadows, A. Arkin, and J.D. Keasling


1170 Molecular Engineering of Exocytic Vesicle Traffi c Enhances the Productivity of Chinese Hamster Ovary CellsR.-W. Peng and M. Fussenegger


1182 A Study of Monoclonal Antibody-Producing CHO Cell Lines: What Makes a Stable High Producer?J. Chusainow, Y.S. Yang, J.H.M. Yeo, P.C. Toh, P. Asvadi, N.S.C. Wong, andM.G.S. Yap


Engineering Science of Biological Systems

1197 Tricalcium Phosphate Nanoparticles Enable Rapid Purifi cation, Increase Transduction Kinetics, and Modify the Tropism of Mammalian VirusesI.A.J. Dreesen, N.A. Lüchinger, W.J. Stark, and M. Fussenegger


Synthetic Biology

1209 Shifting of the Distribution of Aromatic Monomer-Units in Polyhydroxyalkanoic Acid to Longer Units by Salicylic Acid in Pseudomonas fl uorescens BM07 Grown With Mixtures of Fructose and 11-Phenoxyundecanoic AcidM.H. Choi, J. Xu, J.K. Rho, J.H. Shim, and S.C. Yoon


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Tissue Engineering and Delivery Systems

1222 Design of Well and Groove Microchannel Bioreactors for Cell CultureN. Korin, A. Bransky, M. Khoury, U. Dinnar, and S. Levenberg


1231 Controlling Cell Position in Complex Heterotypic 3D Microtissues by Tissue FusionA.P. Rago, D.M. Dean, and J.R. Morgan


1242 Cross-Linking Density Alters Early Metabolic Activities in Chondrocytes Encapsulated in Poly(Ethylene Glycol) Hydrogels and Cultured in the Rotating Wall VesselI. Villanueva, B.J. Klement, D. von Deutsch, and S.J. Bryant


1251 Engineering and Expression of a Chimeric Transferrin Receptor Monoclonal Antibody for Blood–Brain Barrier Delivery in the MouseR.J. Boado, Y. Zhang, Y. Wang, and W.M. Pardridge


1259 An Injectable Cellulose-Based Hydrogel for the Transfer of Autologous Nasal Chondrocytes in Articular Cartilage DefectsC. Vinatier, O. Gauthier, A. Fatimi, C. Merceron, M. Masson, A. Moreau, F. Moreau, B. Fellah, P. Weiss, and J. Guicheux


COMMUNICATIONS TO THE EDITOR

Biocatalysis, Protein Engineering, and Nanobiotechnology

1268 The Activity of Human CYP2D6 in Low Water Organic SolventsJ. Zhao and K. Auclair


1273 Effect of Sodium Dodecylbenzene Sulfonate on Subtilisin Carlsberg Proteolysis of an Immobilized Ovalbumin FilmL.L. Foose, H.W. Blanch, and C.J. Radke


Cellular and Metabolic Engineering

1278 Selective Separation and Co-Culture of Cells by Photo-Induced Enhancement of Cell Adhesion (PIECA)J.-i. Edahiro, K. Sumaru, Y. Ooshima, and T. Kanamori


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Cover Legend

Fusion of cellular tissues, created by a micromold, was investigated by SEM and fl uorescent microscopy. The investigation has implications in developmental biology and tissue engineering. (Picture courtesy of Adam Rago, Dylan Dean, and Jeffrey Morgan.)

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