BIOSOL SL36 DEMONSTRATION PROJECT: ZAKHELE VIP …

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for consideration to

JOE GQABI DISTRICT

MUNICIPALITY

BIOSOL SL36 DEMONSTRATION PROJECT: ZAKHELE

VIP TOILETS AND BURGERSDORP WASTEWATER

TREATMENT WORKS

MARCH 2018

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<< Page left intentionally blank >>

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BIOSOL SL36 DEMONSTRATION PROJECT:

ZAKHELE VIP TOILETS AND BURGERSDORP

WASTEWATER TREATMENT WORKS

MARCH 2018

Produced by BioPower Corporation and Lugaju

Innovation

BioPower Corporation

141 Cresswell Road,

Weavind Park, Pretoria

South Africa

P.O. Box 973

Silverton

Pretoria, 0127

+27 (0) 12 804 9907

[email protected]

www.biopowercorp.co.za

Reg. Number: 2013/061017/07 VAT Number: 4420264162

Directors: T.Z. Phakathi, P.J.D. Germishuys, J.P. Ferreira

Lugaju Innovations

21 St Georges Street

Southernwood, East London

South Africa

21 St Georges Street

Southernwood, East London

South Africa

+27 (0) 43 722 2130

[email protected]

www.lugaju.co.za

Reg. Number: 2014/118383/07

mailto:[email protected]

http://www.biopowercorp.co.za/


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TABLE OF CONTENTS

1. INTRODUCTION ............................................................................................................ 7

2. PROBLEM STATEMENT .................................................................................................. 7

3. DEMONSTRATION PROJECT DETAILS .......................................................................... 8

4.1 VIP Demonstration Project at Zakhele (Rhodes) ................................................... 8

4.1.1. 28 February 2018 ......................................................................................................... 8

4.1.2. 08 March 2018 ............................................................................................................. 9

4.1.3. 15 March 2018 ........................................................................................................... 11

4.1.4. 22 March 2018 ........................................................................................................... 13

4.1.5. Sludge Measurements at Zakhele/Rhodes .............................................................. 14

4.1.6. Identified Benefits of BioSol SL36 vs Vacuum Truck Emptying ................................. 14

4.1.7. Work Creation through Pit Latrine Treatment .......................................................... 16

4.2 Wastewater Treatment Works Demonstration at Burgersdorp ............................ 16

4. SUMMARY OF ANNEXURES ....................................................................................... 19

5. CONCLUSION ............................................................................................................ 19

6. SIGN-OFF ...................................................................... Error! Bookmark not defined.

ANNEXURE 1: PIT LATRINE TREATMENT SCHEDULES ........................................................ 20

1. 28 February 2018.................................................................................................... 20 2. 08 March 2018........................................................................................................ 21 3. 15 March 2018........................................................................................................ 22 4. 22 March 2018........................................................................................................ 23

ANNEXURE 2: ATTENDANCE REGISTERS .......................................................................... 24

1. 28 February 2018.................................................................................................... 24 2. 08 March 2018........................................................................................................ 25 3. 15 March 2018........................................................................................................ 26 4. 22 March 2018........................................................................................................ 27

ANNEXURE 3: BURGERSDORP EFFLUENT ……………………………………………………28

1. Values ………………………………………………………………………………………28

2. Ratios …………………………………………………….………………………………….30

3. Interpretation ………………………………………………………………………………32

ANNEXURE 4: MEETING MINUTES (28 FEBRUARY 2018) .................................................. 52

ANNEXURE 5: DEMONSTRATION AND DOCUMENT SIGN-OFF ...................................... 55

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1. INTRODUCTION

After the initial BioPower Corporation-Lugaju engagement with Joe Gqabi District

Municipality on 24 January 2018, the company initialised a detailed proposal on the

maintenance of pit latrines both at personal dwellings and at schools in the municipality,

as well as the use of BioSol SL36 in Wastewater Treatment Works. After follow-up

engagements with JoGEDA, BioPower was tasked to do a VIP treatment demonstration at

Rhodes (Zakhele) for three (3) weeks and a Wastewater Treatment Works demonstration

at Burgersdorp for six (6) weeks.

2. PROBLEM STATEMENT

During the desktop assessments conducted on:

Wastewater Treatment Works (WWTWs)

Private Pit Latrine maintenance

Basic Sanitation at Rural Schools

Water use in Urban Schools

Informal settlements in Joe Gqabi District Municipality

the following challenges were observed:

There are 48 769 pit latrines in the district in dire need of maintenance. These

private Pit latrines are full, and no sustainable solution has been found to maintain

them for the health benefit of the community. Desludging through Vacuum Trucks

is an option, but this is not sustainable in the long run, and leaves the possibility for

spillage.

There are still 125 schools within the Joe Gqabi District Municipality borders that

have pit latrines. After legislation was passed in 2016 making this a cause of

concern, the aim is now to supply proper sanitation systems for these schools.

Although WWTWs in Joe Gqabi are generally performing better, most plants are

still underperforming. There is definitely room for improvement, especially on

effluent quality, technical skills compliance and flow data general management.

During the 2013 cycle of the Green Drop Report, no (0) plants achieved compliance

greater than 85% on the parameters monitored, thus contributing to a higher risk

rating of each plant.

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3. DEMONSTRATION PROJECT DETAILS

4.1 VIP Demonstration Project at Zakhele (Rhodes)

On the 28th of February 2018, BioPower met with representatives from Joe Gqabi

District Municipality at Rhodes to start the first treatment of VIP toilets which would

be carried out weekly for three (3) weeks. (Minutes attached as Annexure 1)

Ten (10) VIP toilets were identified on site after the conclusion of the meeting, and

treatment commenced. Vuyani is the local person identified to assist with the

demonstration.

4.1.1. 28 February 2018

It was decided that his VIP would be the first to be treated, with a further nine

(9) identified and treated.

As summarised below, the following challenges were experienced:

A lot of foreign objects were discovered inside the VIP pits treated. These

included plastics, tins, paper, bottles etc.

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The VIP at Stand 332 had also experienced structural issues, and has

started to sink, leaning backwards.

The last VIP treated (Stand 337) had pipe leakage draining into the pit of

the toilet.

Odours emanating from these toilets were very bad as can be surmised

from the odours scale in the table attached as Annexure 1.

4.1.2. 08 March 2018

During the second treatment cycle more than 20mm of rain was experienced

at Rhodes/Zakhele, impeding the treatment scheduled. According to people

from the community that has rain gauges, more than 76mm of rain has fallen

during the month to date. Only six (6) toilets were measured and treated due

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to excessive rains impeding the treatment, with the four (4) outstanding VIPs

treated the next day on the 9th of March.

Even the toilets treated had evidence of spillage into the pits from the

adverse weather conditions. The odours were in the medium range at all the

VIPs, and with the addition of BioSol SL36 it reduced more immediately.

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4.1.3. 15 March 2018

As per arrangement, BioPower Corporation again met with JoGEDA and Joe

Gqabi District Municipality on the 15th of March for the follow-up treatment

and measurement as scheduled.

As before, Rhodes was extremely wet, with 40mm measured in a single hour

on the 14th of March and some scattered showers since the previous week’s

treatment.

The treatment had gone extremely well up to this point, and huge gains can

be seen through the table below. The only outliers were the VIPs that

received an excessive amount of rain water which interfered with the

measurements.

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A lot of these VIPs are sub-par, with structures being dilapidated, further

exasperating the situation. The pits are also unsealed, leaving the wastewater

to soak away into the soil thereby contaminating the water table. Through

the gains made with the BioSol SL36, the effect of the soakaway would be

one of remediation at the sub-surface water level.

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4.1.4. 22 March 2018

On 22 March 2018, BioPower Corporation conducted the final treatment and

measurement cycle on the ten (10) toilets at Zakhele. More rain fell in the

Rhodes area during the past week, with the same consequences as before.

The seepage from the rain into the pits leave measurements to be over-

inflated as compared to the actual level, but as a company BioPower

Corporation is extremely satisfied with the results achieved.

According to the locals, around 59mm of rain was measured since the

previous treatment date – this takes the total rain experienced since the start

of treatment to around 180mm.

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4.1.5. Sludge Measurements at Zakhele/Rhodes

Stand

Number

Sludge Level from Top (in millimetres)

2018/02/28 2018/03/08 2018/03/15 2018/03/22 Cumulative Reduction

372 1 480 mm 1 530 mm 1 640 mm 1 710 mm 230 mm

373 1 180 mm 1 240 mm 1 280 mm 1 340 mm 160 mm

355 1 850 mm 1 670 mm* 1 670 mm* 1 650 mm* -200 mm*

376 1 390 mm 1 570 mm 1 680 mm 1 680 mm 290 mm

354 1 230 mm 1 290 mm 1 320 mm 1 340 mm 110 mm

353 870 mm 790 mm* 800 mm* 890 mm* 20 mm*

335* 1 090 mm 1 290 mm 1 340 mm 250 mm

332* 1 090 mm 1 250 mm 1 290 mm 200 mm

336* 1 390 mm 1 500 mm 1 630 mm 240 mm

337* 1 030 mm 1 220 mm 1 320 mm 290 mm

TOTAL AVERAGE DROP (EXCL. STANDS 355 AND 353) 221 mm

*As noted above, due to the gradient in Zakhele the last four (4) toilets were flooded

with rainwater and treatment not possible. Stands in the 330’s and 350’s were

affected and can be evidenced in the measurements of Stands 353 and 355 as well.

4.1.6. Identified Benefits of BioSol SL36 vs Vacuum Truck Emptying

4.1.6.1. Accessibility of BioSol SL36 Treatment

Some of the deep rural areas are inaccessible- or at the very least difficult

to access with vacuum trucks, making the emptying of these facilities

very difficult.

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With BioSol SL36 treatment, accessibility is much less of a concern thanks

to the product being supplied in 20ℓ containers, which are much easier

to get to any area in need, even where no access roads are available as

well as the use of localised labour.

4.1.6.2. Potential Health- and Environmental Risks

Millions of litres of sludge have been emptied from rural pit latrine/septic

tanks in the Eastern Cape during 2017. Generally, the vacuum trucks are

then emptied into municipal Wastewater Treatment Works, where the

addition of chemicals to the toilets then enter these works, contributing

to the dysfunction of many of these plants.

Even more alarming is the allegations that many of these tankers empty

their loads into streams and rivers.

Both of the above scenarios hold an inherent health risk for the

communities that live downstream from the discharge, as well as an

environmental risk due to contamination.

One of the main benefits of BioSol SL36 is that it digests the sludge and

treats it inside the pits, therefore eliminating the need to transport the

faecal sludge and risking contamination and spillage.

4.1.6.3. Other Environmental Risks and Efficiency of Vacuum Trucks

What has been observed by BioPower Corporation over the past few

years is the inefficiency of the method employed to empty pit latrines

nationwide.

The tanker companies (or the community on instruction) add between

20- and 125 litres of fresh water to their pit latrines/VIPs. This firstly

contaminates potable water that could have been used for drinking,

showering or cooking. Thereafter, the vacuum trucks empty the pits –

this is generally only the water that was specifically added, leaving

behind the settled sludge, and thereby have very little impact on the

actual challenge of sludged up pit latrines.

So not only does the method prove ineffective, but it also pollutes

millions of litres of drinkable water in the process.

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4.1.7. Work Creation through Pit Latrine Treatment

Due to the difficulty in managing human waste, pit latrines very often have

bad odours and can propagate diseases like cholera and diarrhoea. BioPower

Corporation Group uses local job creation in a model to treat these pit

latrines with BioSol SL36.

Employment of up to 206 people per 20 000 pit latrines treated per month is

catered for through this Pit Latrine Treatment Work Creation model, which

ties up with either co-ops, SMME’s or government programmes like EPWP or

CWP.

With more than 48 000 pit latrines in the District Municipality, close to 500

sustainable job opportunities can be created.

4.2 Wastewater Treatment Works Demonstration at Burgersdorp

On the 27th of February a meeting was held between BioPower Corporation,

JoGEDA and Adam Potgieter from Joe Gqabi District Municipality. Mr Potgieter is

the official in charge of the Burgersdorp Wastewater Treatment Works. BioPower

Corporation and BioSol SL36 were introduced to him, as well as the current status

of the demonstration project on Burgersdorp WWTW between BioPower/JGDM

and JoGEDA. Mr Potgieter indicated that he will do the ten (10) litres daily dosing

himself to ensure that procedures are followed. Twenty (20) litres of BioSol SL36

has been applied already by BioPower Corporation on that day. The total BioSol

SL36 made provision for is 470 litres over 6 weeks, with Mr Potgieter starting

treatment on the 28th of February 2018.

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After the follow-up engagement between BioPower Corporation and JoGEDA on 15

February 2018, the company was given permission to perform a detailed

assessment on Burgersdorp WWTW. This document was compiled from the reports

below and submitted:

Green Drop Report 2013

Green Drop Progress Report 2014

Burgersdorp WWTW: Preliminary Assessment

Joe Gqabi DM: Project Proposal and Business Case 2018

Physical site assessment

Due to lots of mechanical failure, it was noted that Burgersdorp WWTW might not

be the ideal demonstration site for BioSol SL36 treatment – the challenge was

however accepted. Test results have shown the efficiency of the BioSol SL36

already, and odours are gone.

It should be noted that after treatment with BioSol SL36, the effluent will be of

superior quality and have no adverse effects on human- or environmental health,

and be safe to use at the golf course – while still remaining grey water and not fit

for consumption.

On the 15th and 22nd of March 2018, Burgersdorp was again inspected by BioPower

Corporation and it was noted that there was no adverse odour at the plant, and –

as above photos – the colour of the water was brown, indicating the settled solids

are digesting at a rapid rate.

As testimonial received verbally from Doortjies, the plant supervisor, they have

never been able to see the chlorine pucks so clearly at the outflow, and the odour

reduction makes it much more beneficial for work.

Before treatment with BioSol SL36 (no

aeration due to lack of inflow)

After less than two (2) weeks of

treatment with BioSol SL36 (aeration)

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Effluent analysis has been received from Joe Gqabi District Municipality as

requested, and laid out in Annexure 4.

Before treatment with BioSol SL36 (notice

the suspended solids)

After three (3) weeks of treatment with

BioSol SL36 (no solids/clearer water)

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4. SUMMARY OF ANNEXURES

Annexure 1: Pit Latrine Treatment Schedules

Annexure 2: Attendance Registers

Annexure 3: Burgersdorp Wastewater Analysis

Annexure 4: Meeting Minutes (28 February 2018)

Annexure 5: Demonstration Project Sign-off

5. CONCLUSION

It should be noted that the treatment of pit latrines is not a once-off process, but rather

a continuous programme that needs to be managed in a professional manner.

It is therefore essential for the local community to be committed to the task at hand,

and to ensure a high level of management and performance across the different areas.

We are confident that, not just Joe Gqabi District Municipality, but the country in whole

will benefit from our unique offering which improves the quality and lives of people,

whilst at same time protecting water resources and the environment. Local community

members will also have a chance to improve their lives and those of their families.

To evolve the Private Pit Latrine Treatment to its full capacity, it is planned to

implement it in every region throughout the country.

With support and assistance from other stakeholders, such as National and Local

Government, we will have a positive impact on the entire country and the continent.

Produced by BioPower Corporation (Pty) Ltd: March 2018

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ANNEXURE 1: PIT LATRINE TREATMENT SCHEDULES

1. 28 February 2018

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2. 08 March 2018

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3. 15 March 2018

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4. 22 March 2018

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ANNEXURE 2: ATTENDANCE REGISTERS

1. 28 February 2018

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2. 08 March 2018

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3. 15 March 2018

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4. 22 March 2018

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ANNEXURE 3: BURGERSDORP EFFLUENT

1. Values

These are the actual effluent values of the tests done, with the maximum General

Releasing Standards indicated as a red dashed line, and the BioSol SL36 start date

indicated in a green dashed line. Here, the lower the value the better.

BioSol SL36 start date

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2. Ratios

Below graphs depict the RATIO of inflow vs outflow, in other words, how much lower

the counts in the effluent is when compared to the inflow – or the rate of improvement,

therefor the higher the better. Red dashes indicate the BioSol SL36 start date.


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3. Interpretation

Chemical Oxygen Demand

Biodegradable organics are principally composed of proteins, carbohydrates and fats, and

are commonly measured in terms of Chemical Oxygen Demand (COD). If discharged

untreated to the environment, their biological stabilisation can lead to depleted oxygen

levels and the development of septic conditions. If the COD exceeds the required limits,

intervention is required to rectify the situation (e.g. optimise operation at the WWTW).

CHEMICAL OXYGEN DEMAND (COD): Results and Release Limits

Date Influent Results

(mg/l) Effluent Results

(mg/l) General Release

Limit (mg/l)[1] Special Release Limit (mg/l)[1]

January 16, 2018 577 mg/l 129 mg/l 75 mg/l 30 mg/l

February 6, 2018 51.4 mg/l 188 mg/l 75 mg/l 30 mg/l

February 19, 2018 380 mg/l 146 mg/l 75 mg/l 30 mg/l

March 6, 2018[2] 518 mg/l 96.4 mg/l 75 mg/l 30 mg/l

March 19, 2018[2] 410 mg/l 44.5 mg/l 75 mg/l 30 mg/l [1] After removal of algae

[2] Red fonts indicate tests done after the addition of BioSol SL36

As can be seen in the above table and accompanying graphs, COD results reached up to

250% of the General Release Limit before the addition of BioSol SL36, where after it

recovered to nearly 60% of the General Release Limit after less than three (3) weeks.

This is well within compliance levels, and indicates the immense change BioSol SL36 has

made to this important factor in wastewater treatment in a very short space of time.

Total Suspended Solids (0.45µm)

Suspended solids can lead to the development of sludge deposits and anaerobic

conditions when untreated wastewater is discharged to the aquatic environment. If the

suspended solids concentration exceeds the required limits, intervention is required to

rectify the situation (e.g. ensure source protection, optimise operation at the WWTW).

TOTAL SUSPENDED SOLIDS (TSS): Results and Release Limits




Limit (mg/l) Special Release

Limit (mg/l)

January 16, 2018 218 mg/l 35 mg/l 25 mg/l 10 mg/l


February 19, 2018 No information 25 mg/l 10 mg/l

March 6, 2018[1] 154 mg/l 17 mg/l 25 mg/l 10 mg/l

March 19, 2018[1] 148 mg/l 13 mg/l 25 mg/l 10 mg/l [1]

Red fonts indicate tests done after the addition of BioSol SL36

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From the above table and supporting graphs, the decrease of TSS after just three (3)

weeks have brought the counts very close to the Special Release Limits.

Electrical Conductivity

Electrical Conductivity (EC) is the measure of the ease with which water conducts

electricity and gives an indication of the total dissolved salt (TDS) content of the water.

Health effects related to EC occur only at levels above about 370 mS/m. Adverse effects

may include disturbance of salt and water balance in infants, heart patients, individuals

with high blood pressure, and renal disease. The EC measurement (in mS/m) can also be

used to estimate the TDS (in mg/L). If the EC exceeds the required limits, intervention is

required to rectify the situation (e.g. ensure source protection, ensure treatment plant

can effectively reduce EC/TDS, optimise operation at the treatment plant).

ELECTRICAL CONDUCTIVITY (EC): Results and Release Limits


(mS/m) Effluent Results

(mS/m) General Release

Limit (mS/m) Special Release

Limit (mS/m)

January 16, 2018 116 mS/m 105 mS/m 70 mS/m[2] 50 mS/m

February 6, 2018 56.4 mS/m 73.1 mS/m 70 mS/m[2] 50 mS/m

February 19, 2018 71.9 mS/m 73.6 mS/m 70 mS/m[2] 50 mS/m

March 6, 2018[1] 92.1 mS/m 57.3 mS/m 70 mS/m[2] 50 mS/m

March 19, 2018[1] 85.2 mS/m 80.6 mS/m 70 mS/m[2] 50 mS/m [1]


EC range (mS/m)

Usefulness of water

0 - 80 Good drinking water for humans (provided there is no organic pollution and not too much suspended clay material)

Generally good for irrigation, though above 30 mS/m, some care must be taken, particularly with overhead sprinklers which may cause leaf scorch on some salt sensitive plants.

Suitable for all livestock

80 - 250 Can be consumed by humans although most would prefer water in the lower half of this range if available.

When used for irrigation, requires special management including suitable soils, good drainage and consideration of salt tolerance of plants.

250 – 1,000

Not recommended for human consumption, although water up to 300 mS/m could be drunk if nothing else was available.

Not normally suitable for irrigation, though water up to 600 mS/m can be used on very salt tolerant crops with special management techniques. Over 600 mS/m, occasional emergency irrigation may be possible with care, or if sufficient low salinity water is available, this could be mixed with the high salinity water to obtain an acceptable supply.

[2]

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Electrical Conductivity declined sharply on the 6th of March, nearly reaching Special

Release Limits, and then climbed again on the following analysis. This is due to the

working of the BioSol SL36, firstly reducing due to the solids in the water being

digested, and secondly rising again as settled sludge becomes loose.

Ammonia

Nitrogen (along with phosphorous and carbon) are essential nutrients for growth. When

discharged to the aquatic environment, these nutrients can lead to the growth of

undesirable aquatic life. In a wastewater treatment plant, ammonia is normally oxidised

to nitrites and then to nitrates. A low ammonia concentration in final treated

wastewater effluent therefore normally indicates that effective nitrification has

occurred within the wastewater treatment process. Considering the above, ammonia

measured in drinking-water may indicate the presence of untreated sewage. If the

ammonia concentration exceeds the required limits, intervention is required to rectify

the situation (e.g. optimise operation at the WWTW).

AMMONIA (DISSOLVED): Results and Release Limits




Limit (mg/l) Special Release

Limit (mg/l)

January 16, 2018 53.6 mg/l 17.7 mg/l 6 mg/l 2 mg/l


February 19, 2018 22 mg/l 6.27 mg/l 6 mg/l 2 mg/l

March 6, 2018[1] 22 mg/l 5.05 mg/l 6 mg/l 2 mg/l

March 19, 2018[1] 46.3 mg/l 0.075 mg/l 6 mg/l 2 mg/l [1] Red fonts indicate tests done after the addition of BioSol SL36

Ammonia counts have dropped exceptionally much since the start of the BioSol SL36

programme, what makes this even more significant is the fact that the last results taken

now falls well within the Special Release Limits.

When used for drinking water by poultry and pigs, the salinity should be limited to about 600 mS/m. Most other stock can use water up to 1,000 mS/m.

High magnesium levels can cause stock health problems in this range. Analysis recommended.

Over 1,000 Not suitable for human consumption or irrigation

Not suitable for pigs, poultry or any lactating animals. Beef cattle can use water up to 1,700 mS/m and adult dry sheep can tolerate 2,300 mS/m. However it is possible that waters below these EC levels could contain unacceptable concentrations of particular ions. Detailed chemical analysis should therefore be considered before using high salinity water for stock.

Water up to 5,000 mS/m (the salinity of the sea), can be used to flush toilets provided corrosion in the cistern can be controlled.

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E.coli

Although E.coli on its own is generally not a threat to human- or environmental health –

the presence of this organism is an indicator that other more harmful bacteria might be

present in the water.

The testing of E.coli is inexpensive when compared to the other thousands of microbes

that would otherwise have to be tested for.

E.COLI: Results and Release Limits

Date Effluent Results

(cfu/100ml) General Release Limit

(cfu/100ml) Special Release Limit

(cfu/100ml)

January 16, 2018 1 203 cfu/100ml 1 000 cfu/100ml 0 cfu/100ml

February 6, 2018 >2 420 cfu/100ml 1 000 cfu/100ml 0 cfu/100ml

February 19, 2018 > 2 420 cfu/100ml 1 000 cfu/100ml 0 cfu/100ml

March 6, 2018[1] >2 420 cfu/100ml 1 000 cfu/100ml 0 cfu/100ml

March 14, 2018[1] 6 cfu/100ml 1 000 cfu/100ml 0 cfu/100ml

March 19, 2018[1] 0 cfu/100ml 1 000 cfu/100ml 0 cfu/100ml [1]


The tests between the 6th of February and the 6th of March is very undefined, with a

count of 2 420 being the minimum E.coli count but no maximum is set due to testing

constraints.

After two (2) weeks of treatment the E.coli counts have dropped to six (6) and less than

a week later to undetectable – which even complied with Special Release Limits. This

amazing result is what sets the BioSol SL36 apart from any other product on the market.

Disclaimer: All tests were performed by Joe Gqabi District Municipality and their

laboratory of choice. BioPower had no input with regards to effluent

analysis, keeping the results completely unbiased. Analyses follow:

Date Completed:

Phone:

SAMPLE IDENTIFICATION

Bongani Makehle

Sample Condition:

Report NO:

Customer:

Address:

20-Nov-2017

Barkly East 9786

Coner Cole and Graham Street

South Africa

Sample Identification:

07-Nov-2017Date Received:

Waste WaterSample Description:

Cold

Contact:

Joe Gqabi District Municipality

Order No: Bongani

T0626

0459793150

No. of Samples 02

Fax: 0459710432

Burgersdorp

Water

Certificate Of AnalysisEE-2017-01374

Name:

Tel:

Email:

Mandla Sodladla

087 3730951/2/3

[email protected]

ELIDZ Environmental Chemistry Laboratory

PO Box 5458, Greenfields

East London, 5208

Lower Chester Road

SunnyRidge, 5201

East London, South Africa

Test Date: 07-Nov-2017

EE-17-04038 EE-17-04039

Burgersdorp

WWTW Inffluent

1356 331/LG/17

Burgersdorp

WWTW Effluent

1356 332/LG/17

Chemical Oxygen Demand mg/L as O₂ MM-CHE003 440 107

Electrical conductivity mS/m MM-CHE002 117 166

Nitrite (dissolved) * mg/L as N MM-CHE011 0.030 0.030

Nitrate as N (dissolved) * mg/L as N MM-CHE010 0.150 13.9

Ammonia (dissolved) mg/L as N MM-CHE004 22.5 <0.070

Ortho phosphate(dissolved) * mg/L as P MM-CHE012 3.61 3.55

pH pH units MM-CHE005 7.69 7.68

Temperature * °C 25.0 25.0

Total suspended solids

(0.45µm) *

mg/L MM-CHE016 130 73.0

Analysis Unit Method

Lab No

Sample ID

Date Printed

20-Nov-2017

Page 1 of 1Mandla Sodladla - Laboratory Supervisor

This report relates only to the samples actually supplied to ELIDZ. The operation unit does not accept responsibility for any matters arising from the further use of these

results. This certificate shall not be reproduced, except in full, without the written approval of the Laboratory Manager. No reference may be made to the ELIDZ or any of its

operation units or officers in advertisements or for sale or publicity purposes without the ELIDZ's prior approval. All work is undertaken according to the ELIDZ general

conditions of contract. Samples may be discarded two weeks from the issue date of this certificate so please notify us within this time if you have any comments or queries

about this certificate or results.

Remarks: * This is not SANAS accredited and is not included in the SANAS Schedule of accreditation for this laboratory.

A copy of the original of this certificate is available from the ELIDZ on request. This certificate is issued without any alteration or erasure.

Date Completed:

Phone:


Bongani Makehle

Sample Condition:

Report NO:

Customer:

Address:

10-Nov-2017

Barkly East 9786


South Africa




Cold

Contact:


Order No: Bongani

T0626

0459793150

No. of Samples 01

Fax: 0459710432

Burgersdorp

Water

Certificate Of AnalysisEM-2017-02007

Name:

Tel:

Email:

Mandla Sodladla

087 3730951/2/3

[email protected]

ELIDZ Environmental Microbiology


East London, 5208

Lower Chester Road

SunnyRidge, 5201



EM-17-06416

Burgersdorp

WWTW Ecoli Test

1356 333/LG/17

E. coli MPN/100mL MM-MIC002 <1


Lab No

Sample ID

Date Printed

10-Nov-2017









Date Completed:

Phone:


Bongani Makehle

Sample Condition:

Report NO:

Customer:

Address:

29-Nov-2017

Barkly East 9786


South Africa




Cold

Contact:


Order No: Bongani

T0626

0459793150

No. of Samples 02

Fax: 0459710432

Burgersdorp

Water


Name:

Tel:

Email:

Mandla Sodladla

087 3730951/2/3

[email protected]



East London, 5208

Lower Chester Road

SunnyRidge, 5201



EE-17-04219 EE-17-04220

Burgersdorp

WWTW Inffluent

1356 344/LG/17

Burgersdorp

WWTW Effluent

1356 345/LG/17



Nitrite (dissolved) * mg/L as N MM-CHE011 <0.012 <0.012


Ammonia (dissolved) mg/L as N MM-CHE004 22.3 <0.070





(0.45µm) *

mg/L MM-CHE016 174 41.0


Lab No

Sample ID

Date Printed

29-Nov-2017









Date Completed:

Phone:


Bongani Makehle

Sample Condition:

Report NO:

Customer:

Address:

27-Nov-2017

Barkly East 9786


South Africa



Portable WaterSample Description:

Cold

Contact:


Order No: Bongani

T0626

0459793150

No. of Samples 01

Fax: 0459710432

Burgersdorp

Water


Name:

Tel:

Email:

Mandla Sodladla

087 3730951/2/3

[email protected]



East London, 5208

Lower Chester Road

SunnyRidge, 5201



EM-17-06678

Burgersdorp

WWTW Ecoli Test

1356 346/LG/17

E. coli MPN/100mL MM-MIC002 <1


Lab No

Sample ID

Date Printed

27-Nov-2017









Date Completed:

Phone:


Bongani Makehle

Sample Condition:

Report NO:

Customer:

Address:

22-Jan-2018

Barkly East 9786


South Africa


16-Jan-2018Date Received:


Cold

Contact:


Order No: Bongani

T0626

0459793150

No. of Samples 02

Fax: 0459710432

Burgersdorp

Water


Name:

Tel:

Email:

Mandla Sodladla

087 3730951/2/3

[email protected]



East London, 5208

Lower Chester Road

SunnyRidge, 5201


Test Date: 16-Jan-2018

EE-18-04765 EE-18-04766

Burgersdorp

WWTW Inffluent

1356 11/AM/17

Burgersdorp

WWTW Effluent

1356 12/AM/17



Nitrite (dissolved) * mg/L as N MM-CHE011 <0.012 3.01

Nitrate as N (dissolved) * mg/L as N MM-CHE010 <0.030 7.90

Ammonia (dissolved) mg/L as N MM-CHE004 53.6 17.7





(0.45µm) *

mg/L MM-CHE016 218 35.0


Lab No

Sample ID

Date Printed

22-Jan-2018









Date Completed:

Phone:


Bongani Makehle

Sample Condition:

Report NO:

Customer:

Address:

22-Jan-2018

Barkly East 9786


South Africa


16-Jan-2018Date Received:


Cold

Contact:


Order No: Bongani

T0626

0459793150

No. of Samples 01

Fax: 0459710432

Burgersdorp

Water


Name:

Tel:

Email:

Mandla Sodladla

087 3730951/2/3

[email protected]



East London, 5208

Lower Chester Road

SunnyRidge, 5201


Test Date: 16-Jan-2018

EM-18-07341

Burgersdorp

WWTW Ecoli Test

1356 13/AM/17

E. coli MPN/100mL MM-MIC002 1203


Lab No

Sample ID

Date Printed

22-Jan-2018









Date Completed:

Phone:


Bongani Makehle

Sample Condition:

Report NO:

Customer:

Address:

12-Feb-2018

Barkly East 9786


South Africa


06-Feb-2018Date Received:


Cold

Contact:


Order No: Bongani

T0626

0459793150

No. of Samples 01

Fax: 0459710432

Burgersdorp

Water


Name:

Tel:

Email:

Mandla Sodladla

087 3730951/2/3

[email protected]



East London, 5208

Lower Chester Road

SunnyRidge, 5201


Test Date: 06-Feb-2018

EM-18-07612

Burgersdorp

WWTW Ecoli Test

1356 24/LG/18

E. coli MPN/100mL MM-MIC002 >2420


Lab No

Sample ID

Date Printed

12-Feb-2018









Date Completed:

Phone:


Mandla Sodladla

Sample Condition:

Report NO:

Customer:

Address:

Barkly East 9786


South Africa




Cold

Contact:


Order No: Bongani

T0626

0459793150

No. of Samples 02

Fax: 0459710432

Burgersdorp

Water

Prelim Certificate Of AnalysisEE-2018-01677

Name:

Tel:

Email:

Mandla Sodladla

087 3730951/2/3

[email protected]



East London, 5208

Lower Chester Road

SunnyRidge, 5201



EE-18-05173 EE-18-05174

Burgersdorp

WWTW- Inffluent

1356 45/LG/18

Burgersdorp

WWTW- Effluent

1356 46/LG/18


Electrical conductivity mS/m MM-CHE002 71.9 73.6


Nitrate as N (dissolved) * mg/L as N MM-CHE010 <0.030 4.12





Lab No

Sample ID

Date Printed

27-Feb-2018

Page 1 of 1








Date Completed:

Phone:


Bongani Makehle

Sample Condition:

Report NO:

Customer:

Address:

Barkly East 9786


South Africa




Cold

Contact:


Order No: Bongani

T0626

0459793150

No. of Samples 01

Fax: 0459710432

Burgersdorp

Water

Prelim Certificate Of AnalysisEM-2018-02406

Name:

Tel:

Email:

Mandla Sodladla

087 3730951/2/3

[email protected]



East London, 5208

Lower Chester Road

SunnyRidge, 5201



EM-18-07950

Burgersdorp

WWTW Ecoli Test

1356 47/LG/18E. coli MPN/100mL MM-MIC002 >2420


Lab No

Sample ID

Date Printed

28-Feb-2018

Page 1 of 1








Date Completed:

Phone:


Bongani Makehle

Sample Condition:

Report NO:

Customer:

Address:

Barkly East 9786


South Africa


06-Mar-2018Date Received:

Waste WasteSample Description:

Cold

Contact:


Order No: Bongani

T0626

0459793150

No. of Samples 02

Fax: 0459710432

Burgersdorp

Water


Name:

Tel:

Email:

Mandla Sodladla

087 3730951/2/3

[email protected]



East London, 5208

Lower Chester Road

SunnyRidge, 5201


Test Date: 06-Mar-2018

EE-18-05246 EE-18-05247

Burgersdorp

WWTW Inffluent

1356 45/AM/18

Burgersdorp

WWTW Effluent

1356 46/AM/18Chemical Oxygen Demand mg/L as O₂ MM-CHE003 518 96.4









(0.45µm) *

mg/L MM-CHE016 154 17.0


Lab No

Sample ID

Date Printed

13-Mar-2018

Page 1 of 1








Date Completed:

Phone:


Bongani Makehle

Sample Condition:

Report NO:

Customer:

Address:

Barkly East 9786


South Africa




Cold

Contact:


Order No: Bongani

T0626

0459793150

No. of Samples 01

Fax: 0459710432

Burgersdorp

Water

Prelim Certificate Of AnalysisEM-2018-02439

Name:

Tel:

Email:

Mandla Sodladla

087 3730951/2/3

[email protected]



East London, 5208

Lower Chester Road

SunnyRidge, 5201



EM-18-08074

Burgersdorp

WWTW Ecoli Test

1356 47/AM/18E. coli MPN/100mL MM-MIC002 >2420


Lab No

Sample ID

Date Printed

13-Mar-2018

Page 1 of 1








Date Completed:

Phone:


Bongani Makehle

Sample Condition:

Report NO:

Customer:

Address:

26-Mar-2018

Barkly East 9786


South Africa




Cold

Contact:


Order No: Bongani

0459793150

No. of Samples 01

Fax: 0459710432

Burgersdorp

Water


Name:

Tel:

Email:

Kholiwe Mgobo

043 1010 164

[email protected]



East London, 5208

Lower Chester Road

SunnyRidge, 5201



EM-18-08149

Burgersdorp

WWTW Ecoli Test

1356 58/AM/18E. coli MPN/100mL MM-MIC002 6


Lab No

Sample ID

Date Printed

27-Mar-2018

Page 1 of 1Kholiwe Mgobo(Cert. Sci.Nat.) - Technical Signatory






Date Completed:

Phone:


Mandla Sodladla

Sample Condition:

Report NO:

Customer:

Address:

Barkly East 9786


South Africa



Waste waterSample Description:

Cold

Contact:


Order No: Bongani

0459793150

No. of Samples 02

Fax: 0459710432

Burgersdorp

Water


Name:

Tel:

Email:

Zolisile Manga

087 3730951/2

043 1010 165



East London, 5208

Lower Chester Road

SunnyRidge, 5201



EE-18-05338 EE-18-05339

BUrgersdorp

WWTW -Inffluent

1356 60/Kem/18

Burgersdorp

WWTW -Effluent

1356 61/Kem/18Chemical Oxygen Demand mg/L as O₂ MM-CHE003 410 44.5


Nitrite (dissolved) mg/L as N MM-CHE011 <0.012 <0.012

Nitrate as N (dissolved) mg/L as N MM-CHE010 0.120 17.7


Ortho phosphate(dissolved) mg/L as P MM-CHE012 4.59 3.18


Temperature °C 25.0 25.0


(0.45µm)

mg/L MM-CHE016 148 13.0


Lab No

Sample ID

Date Printed

28-Mar-2018

Page 1 of 1






Date Completed:

Phone:


Bongani Makehle

Sample Condition:

Report NO:

Customer:

Address:

23-Mar-2018

Barkly East 9786


South Africa




Cold

Contact:


Order No: Bongani

0459793150

No. of Samples 01

Fax: 0459710432

Burgersdorp

Water


Name:

Tel:

Email:

Kholiwe Mgobo

043 1010 164

[email protected]



East London, 5208

Lower Chester Road

SunnyRidge, 5201



EM-18-08250

Burgersdorp

WWTW Ecoli Test

1356 59/KEM/15E. coli MPN/100mL MM-MIC002 <1


Lab No

Sample ID

Date Printed

23-Mar-2018

Page 1 of 1Kholiwe Mgobo(Cert. Sci.Nat.) - Technical Signatory






Meeting name & number

JoGEDA/JGDM/BioPower Start of

Pit Latrine Demo at Zakhele

File reference

BCG-OM-20180228

Location:

Kinmel Lodge, Rhodes

Time: 10:30

BIOPOWER CORPORATION GROUP

and

JOGEDA and JGDM

Date

28 February 2018

Page 55 of 55

ANNEXURE 4: MEETING MINUTES (28 FEBRUARY 2018)

ATTENDEES

NAME INS FIRM EMAIL

Bongani Makehle

Pieter Germishuys

Visa Barnes

Karel McCarthy

Johan Lindeque

BM

PG

VB

KM

JL

JGDM

BCG

JoGEDA

JGDM

BCG

[email protected]

[email protected]

[email protected]

[email protected]

[email protected]

Apologies: None

Circulation: Meeting attendees

All persons present at the meeting will be deemed to have taken notes of such items as concerns them, and failure to receive these minutes cannot be taken as a reason for not taking the necessary action.

1. WELCOME AND APOLOGIES

VB welcomes attendees to the meeting at Kinmel Lodge, Rhodes

to discuss the Pit Latrine Demonstration at Zakhele. VB

2. PURPOSE OF THE MEETING

The purpose of meeting is with regards to the start of the

demonstration project on VIP toilets at Zakhele/Rhodes and how it

will be rolled out.

All









File reference

BCG-OM-20180228

Location:


Time: 10:30


and

JOGEDA and JGDM

Date

28 February 2018

Page 55 of 55

3. DISCUSSION

BM expressed hope that the demonstration will be successful

since the municipality has numerous VIPs, and BioSol SL36

would assist them greatly. There is a lot of groundwater as

well as pristine rivers and streams – this being the reason why

Rhodes/Zakhele was chosen.

PG and VB confirmed that they will be present at each

treatment – currently being set as every Thursday going

forward until the completion of the demonstration. After PG

books his plane tickets it will be formally set as such. KM

indicated that he would also join the treatment dates.

As it stands, the treatment dates will be:

o 8 March

o 15 March

o 22 March

Ten (10) VIP toilets to be identified on site after the conclusion

of the meeting, and treatment to commence. Vuyani is the

local person identified to assist with the demonstration. It was

decided that his VIP would be the first to be treated, with a

further nine (9) to be decided.

PG will bring BioSol stickers for the next treatment date to

clearly mark the toilets used for the demonstration.

FEEDBACK ON BURGERSDORP: PG related that a meeting

was held between himself, VB, JL and Adam Potgieter (AP)

from Joe Gqabi District Municipality. AP is the official in charge

of the Burgersdorp Wastewater Treatment Works. BioPower

Corporation and BioSol SL36 was introduced to him, as well

as the current status of the demonstration project on

Burgersdorp WWTW between BioPower/JGDM and JoGEDA.

AP indicated that he will do the ten (10) litre daily dosing

himself to ensure that procedures are followed. Twenty (20)

litres of BioSol SL36 has been applied already by PG, JL and

VB. The total BioSol SL36 made provision for is 470 litres over

6 weeks.




File reference

BCG-OM-20180228

Location:


Time: 10:30


and

JOGEDA and JGDM

Date

28 February 2018

Page 55 of 55

KM expressed concern that the dysfunction of Burgersdorp

WWTW and rapid flow through of effluent could give BioSol

SL36 too little contact time in the system. JL explained that it

is purely to establish biofilm and a microbiological ecosystem.

All

Smaller dosages each day will be considered with the intention

of moving more product into the system at various intervals.

PG requested if it would be possible for BM to send the

previous two (2) months’ worth of effluent analysis from

Burgersdorp WWTW to be used as a baseline. BM replied in

the affirmative.

All

4. RESOLUTIONS

PG, VB and KM will be at all treatments of the demonstration

project – currently scheduled for Thursdays (refer to above

dates) but to be confirmed after flights are booked.

BM to send PG and JL the previous two (2) months’ worth of

effluent results for Burgersdorp WWTW.

All

5. CLOSING AND NEXT MEETING

Next meeting date to be decided. VB thanked the participants and

the meeting was adjourned. All participants to drive to Zakhele for

the start of the treatment procedures.

VB

____________________________

SIGNATURE: PIETER GERMISHUYS

MANAGING DIRECTOR

of 55

ANNEXURE 5: DEMONSTRATION PROJECT SIGN-OFF

Contractor: BIOPOWER CORPORATION (PTY) LTD

Works: BIOSOL SL36 DEMONSTRATION PROJECTS FOR JOE GQABI DISTRICT MUNICIPALITY AND JoGEDA

Site: BURGERSDORP WWTW and ZAKHELE (RHODES) VIP TOILETS

The involved parties hereby certify that the following criteria were met:

Completion of Demonstration Project/s as agreed at

o Zakhele (Rhodes) VIP toilets, for the period 28 February 2018 – 22 March 2018; o Burgersdorp WWTW, for the period 28 February 2018 – 11 April 2018.

Demonstration Project Report received.

EXECUTED BY BIOPOWER CORPORATION:

Title and Name ………………………………………………………………………………………………..………………………

Signature: ……………………………………….. Date: ……………………………………………..

AND LUGAJU INNOVATIONS (ENVIRONMENTAL EXPERT):

Title and Name ………………………………………………………………………………………………..………………………

Signature: ……………………………………….. Date: ……………………………………………..

IMPLEMENTING AGENT: JoGEDA

Title and Name ………………………………………………………………………………………………..………………………

Signature: ……………………………………….. Date: ……………………………………………..

JOE GQABI DISTRICT MUNICIPALITY

Title and Name ………………………………………………………………………………………………..………………………

Signature: ……………………………………….. Date: ……………………………………………..

BIOSOL SL36 DEMONSTRATION PROJECT: ZAKHELE VIP …

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