PENGANTAR BIOTEKNOLOGI

BIOREMIDIATION

OUTLINE

DEFINITION

PHYTOREMEDIATION

BIOREMEDIATIONTECHNIQUE

CASE STUDY

What is BIOREMEDIATION

The technology used to speed up the natural processesof waste degradation and recycling

Use of naturally occurring microorganism such asbacteria, fungi, and yeast to degrade pollutants orhazardous substances in soil, water and air into non-toxic or less toxic substances in order to return theenvironment to its original natural condition

PLANTS(Phytoremediation)

ENZYME

INORGANICORGANIC

TRIPLE CORNERS PROSESS

POLLUTANS

ORGANISMENVIRONMENT

MICROORGANISMSOILWATERAIR Confining

Unit

Water table

SalineWater

Lateralintrusion ofsaline water

Ocean

Municipalwater well

Abandonedoil well

DeepAquifer

pond

Infiltration ofpesticides andfertilizers from

farmlands

Brine leakage fromruptured well casing

septic tankleakage

Freshwater

Accidentalfuel spill

Municipallandfill

Leakage fromhazardouswaste site

Contaminatedshallow

well

Leakingpetroleum

tank

ConfiningUnit

MICROORGANISM

1- Isolation of the microorganism1- Isolation of the microorganism

5- Determination of the biodegradationefficiency

5- Determination of the biodegradationefficiency

4- Optimization of the biodegradationconditions

4- Optimization of the biodegradationconditions

3- Identification of the microbial isolate3- Identification of the microbial isolate

2- Purification of the obtained isolates2- Purification of the obtained isolates

6- Identification of the biodegradation products.6- Identification of the biodegradation products.

7- Cell or enzyme immobilization.7- Cell or enzyme immobilization.

8- Enzyme identification.8- Enzyme identification.

GMO APPLICATION FORBIOREMIDIATION

Deinococcus radiodurans

organisme paling radioresistantdimodifikasi untuk dapat mencernatoluene dan ionic mercury dari limbahdg kandunga radioactive nuclear yangtinggi

OUTLINE

DEFINITION

PHYTOREMEDIATION

BIOREMEDIATIONTECHNIQUE

CASE STUDY

PHYTOREMEDIATION Phytoremediation is use of plants (HYPERACCUMULATOR

PLANT) for accumulation, removal or conversion ofpollutants.

TUMBUHAN MENARIK ZATKONTAMINAN, BERAKUMULASI DI DAUNTUMBUHAN MENARIK ZATKONTAMINAN, BERAKUMULASI DI DAUN

PENEMPELAN ZAT KONTAMINANPADA AKAR

PENEMPELAN ZAT KONTAMINANPADA AKAR

(Rhyzodegradation)PENGURAIAN ZATKONTAMINAN OLEH AKTIVITAS MO

(Rhyzodegradation)PENGURAIAN ZATKONTAMINAN OLEH AKTIVITAS MO

TRANSPIRASI ZAT KONTAMINANDLM BENTUK MENJADI LARUTAN

TERURAI TDK BERBAHAYA

TRANSPIRASI ZAT KONTAMINANDLM BENTUK MENJADI LARUTAN

TERURAI TDK BERBAHAYA

PENGURAIAN ZATKONTAMINAN

PENGURAIAN ZATKONTAMINAN

HYPERACCUMULATOR PLANT

Can adsorb more than 10.000 ppm for Mn, Zn, Ni

> 1.000 ppm for Cu and Se

> 100 ppm for Cd, Cr, Pb and Co

METAL CONTAMNANTS IN THE SOIL

are ABSORBED by the roots (UPTAKE)

move in to shoot (TRANSLOCATION)

are STORED in the shoot (ACCUMULATION)

Alyssum murale

Nicotina tabbacumNicotina tabbacum

Thiaspi caerulescens

Berkheya spBerkheya sp

FITOREMIDIATION BENEFIT

LOW COST OPERATIONAL

DESCRESE CONTAMINANT POLLUTANSNATURALLY

CONTAMINATED PLANT CAN USED AS FUEL

MYCOREMEDIATION

USE FUNGGI AS DECONTAMINATION AREAL

USED FUNGAL MYCELIA

EX: DIESEL OIL CONTAMINATED AREAINOCULATE WITH OYSTER FUNGI. CANCHANGE PAH (polycyclic aromatichydrocarbon) CO2 + H2O

OUTLINE

DEFINITION

PHYTOREMEDIATION

BIOREMEDIATIONTECHNIQUE

CASE STUDY

IN SITU BIOREMEDIATION

SOIL BIOREMIDIATION(IN SITU PROCESSING)

BIOVENTING

Pemompaan udara danNutrisi

AIR SPARGING

Pememompaan udarauntuk meningkatkanaktifitas degradasi olehmikroba

SOIL BIOREMIDIATION(EX-SITU PROCESSING)

Tanah terkontaminasi diangkat ke dan diperlakukan dipermukaan

SOIL BIOREMIDIATION(EX-SITU PROCESSING)

SLURRY PHASE

menggunakan bioreaktor

SOIL BIOREMIDIATION(COMBINATION IN SITU & EX-SITUPROCESSING)

WATER AND GASBIOREMIDIATION

Biofiltration is a process, in which,microorganisms supported on inertmaterials are used to degrade organicpollutants for air, gas and waterbioremediation.

Types of biofilters:

1- Bioscrubbers.

2- Biotrickling filters.

3- Slow sand or carbon filters.

Bioscrubber filtersBioscrubber filters

OUTLINE

DEFINITION

PHYTOREMEDIATION

BIOREMEDIATIONTECHNIQUE

CASE STUDY

Case Study

Sebuah perusahaan POM BENSIN terbesar di Las Vegas, USAsedang menhadapi masalah serius. Tangki yang ditanam dalamtanah sedalam 10 meter mengalami kebocoran.

Jika Anda sebagai Konsultan, dimintauntuk menyelesaikan masalahperusahaan POM BENSIN, Las Vegas,USA tersebut. Apa yang akan andalakukan ?

1. TRAGEDI MINAMATA2. KASUS LOVE CANAL (AMERIKA

SERIKAT)3. KASUS KABUT DIOXIN DI

SEVESO (ITALIA)4. PT. NEWMONT MINAHASA

RAYA PENCEMARTELUK BUYAT

• JELASKAN KASUS PENCEMARAN TERSEBUT SERTA DAMPAK YANGDITIMBULKAN

• JIKA ANDA SEBAGAI KONSULTAN BIDANG LINGKUNGAN SOLUSIAPAKAH YANG AKAN BERIKAN UNTUK MENGATASI MASALAHTERSEBUT (IDENTIFIKASI POLLUTAN, ENVIRONMENT DANORGANISM)