Biological Molecules 2 2013.ppt
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Transcript of Biological Molecules 2 2013.ppt
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Biomolecules 2 Proteins Pork Chop Willie 1
Biological Molecules 2 Proteins
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Learning outcomes
Describe the basic structure of an amino acid and the formation of polypeptides and proteins (as amino acid monomers linked by peptide bonds in condensation reactions)
Explain the significance of a protein’s primary structure in determining its three-dimensional structure and properties (globular and fibrous proteins and types of bonds involved in three dimensional structure).
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Proteins - Functions
Wide range of Antibodies Enzymes Many hormones
Various proteins make up muscles, ligaments, tendons, Hair Important components of cell membranes
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Building blocks: Amino acids
All proteins composed of Amino acids 20 different amino acids Plants can make all 20 amino acids Animals can only make some, the rest
obtained in their diet (essential amino acids)
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Amino Acids R groups can be any of 20
different forms giving 20 naturally occurring amino acids
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Primary structure
Two amino acids join together in a condensation reaction to form a dipeptide
This process is repeated to form a polypeptide chain
A protein is made up of one or more polypeptide chain
The sequence of amino acids is known as the primary structure
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Secondary structureα-helix
A chain of amino acids may twist to form an α-helix
Within the helix, hydrogen bonds form between C=O of the carboxylic acid and –NH2 of the amine group
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Secondary Structureβ-pleated sheet
Several chains may link together, with hydrogen bonds holding the parallel chains in an arrangement known as β-pleated sheet
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Secondary structure
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Tertiary structure
Polypeptide chains often bend and fold to produce three-dimensional shape.
Chemical bonds and hydrophobic interactions between R group maintain this final tertiary structure of the proteins
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Tertiary structure
An R group is polar when the sharing of electrons within it is not quite even
Polar R groups attract other polar molecules and are therefore hydrophilic (water attracting)
The non-polar groups are hydrophobic (water repelling)
Non-polar hydrophobic R groups are arranged to face inside the proteins excluding water from the centre of the molecule
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Tertiary structure
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Quaternary structure
A protein may be made of several polypeptide chains held together
e.g. haemoglobin, insulin Only proteins with several polypeptide
chains have quaternary structure
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Haemoglobin
Haemoglobin consists of four polypeptide subunits two are called α-chains, the other two are called β- chains
Each unit is held in place by a number of bonds and interactions.
These interactions give the molecule a specific shape.
This shape is important for the molecule to carry out its function.
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Haemoglobin
A specialised part of each polypeptide called a haem group contains an iron(Fe2+) ion.
Haem group gives haemoglobin its colour Each haem group binds with one oxygen
molecule Therefore one haemoglobin molecule
contains four oxygen molecules
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Globular proteins
Folded into a compact spherical shape These proteins are soluble due to the
hydrophilic side chains that project from the outside of the molecule.
This make them important in metabolic reactions.
Enzymes are globular proteins
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Globular Proteins
Enzymes three dimensional shape is crucial to their ability to form enzyme substrate complexes and catalyse reactions in cells.
Three dimensional shape is critical in their role of binding to substances. e.g proteins in membranes
Antibodies are also globular proteins and rely on shape to bind to microorganisms
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Fibrous proteins
Do not fold in to ball shape but remain as long chains
Several chains can be cross-linked for additional strength.
These insoluble proteins are important structural molecules.
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Fibrous Proteins
Keratin in Hair and Skin. Collagen in skin. Tendons Bones Cartilage Blood vessel walls
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Fibrous Protein:Collagen
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Collagen
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Denaturation
If proteins are heated or the pH is changed they can loose their shape and unfold.
Such proteins are said to be denatured. In a cell denatured proteins are broken up
and the amino acids reused unless they can be refolded.
Protein aggregates are toxic and accumulations can causes diseases e.g. Altzheimer’s disease, Scrapie
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Enzymes
Many proteins catalyse chemical reactions.
These proteins are known as enzymes.
All enzymes have a definite shape and have a part known as the active site where the substrate(s) bind.
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