Biological Frameworks for Engineersfaculty.washington.edu/nsniadec/ME411/A14/Slides9.pdf ·...
Transcript of Biological Frameworks for Engineersfaculty.washington.edu/nsniadec/ME411/A14/Slides9.pdf ·...
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ME 411 / ME 511
Biological Frameworks
for Engineers
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Class Organization
• Lab 1 worksheet due Wed
• Lab 2 – Lab-on-a-Chip
– Fri, More 320
– Sign up for 2:00-3:15, 3:15-4:30 slots
– Max 20 people per slot
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ME 411 / ME 511
Decoding Proteins and
Protein Functions
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A cell is a crowded place…
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Protein Purification
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Gel Electrophoresis
• Separation by charge-
to-mass ratio
• Small proteins migrate
faster than large ones
• SDS-PAGE
– Sodium dodecylsulfate
– Polyacrylamide Gel
Electrophoresis
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2-D Gel Electrophoresis
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Chromatography
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Western Blot
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Mass Spectrometry
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All together now…
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X-Ray Crystalography
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Cryo-EM
E. coli Ribosome
Tungsten filament (cathode)
Anode
Condenser lens
Electron beam
Specimen
EM Condenser lens
Projector lens
Detector
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NMR Spectroscopy
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Protein Function
• Pharmacological inhibitors
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Protein Function
• Recombinant DNA
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Protein Function
• Knock-out mice
Blastocyte
neo’ = neomycin
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Protein Function
• RNA Interference
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Protein Function
• Green Fluorescent Protein (GFP)
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Questions ?