Biological clock An innate mechanism in living organisms that controls the periodicity or rhythm of...
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![Page 1: Biological clock An innate mechanism in living organisms that controls the periodicity or rhythm of various physiological functions or activities. –Circadian.](https://reader036.fdocuments.us/reader036/viewer/2022062423/5697c00b1a28abf838cc864e/html5/thumbnails/1.jpg)
Biological clockAn innate mechanism in living organisms that controls the periodicity or rhythm of various physiological functions or activities.
– Circadian rhythm• Circadian: circa(around)+dies(day)
– Ultradian rhythm– Infradian rhythm
Internal biological clock+ change of environment
Reset / entrainment mechanism
![Page 2: Biological clock An innate mechanism in living organisms that controls the periodicity or rhythm of various physiological functions or activities. –Circadian.](https://reader036.fdocuments.us/reader036/viewer/2022062423/5697c00b1a28abf838cc864e/html5/thumbnails/2.jpg)
Clock-associated genes
• CCA1• LHY• FKF1• ZTL
Environments
• Temperature
• Light– Period– wave
Relationship clock-associated genes and the photoreceptors
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ELF3 Encodes a Circadian Clock-Regulated Nuclear Protein That
Functions in an Arabidopsis PHYB Signal Transduction Pathway
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elf3
• Defective in light perception or light-mediated signal transduction
• Defective in circadian rhythm responses
wild(C)
elf3-1
elf3-1:cosmid B8
elf3-1:cosmid E11
SD
LDLD
SD
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Two types of photoreceptor
• Five red/far-red light-absorbing phytochromes– Phytochrome A[PHYA], PHYB, PHYC,
PHYD, and PHYE
• Two blue/UV-A light-absorbing cryptochromes– CRY1 and CRY2
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ELF3
• novel 695 amino acid protein• without any significant homology to
proteins of known function• glutamine/threonine-rich sequence
and nuclear localization signal in the C-terminal region
transcription factor
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Fig 1-A. Sequence comparison of ELF3 and putative ELF3 homologs
Cardamine
Arabidopsis
tomatoricemaize
ELF3 function may be conserved between dicots and monocots.
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Fig 1-B. ELF3 is a novel nuclear protein.
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Fig 2. Accumulation of the ELF3 protein is regulated by light and the circadian clock.
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Fig 3-1. ELF3 and PHYB proteins interaction in the Yeast Tow-Hybrid System
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Fig 3-2. ELF3 and PHYB proteins interaction in Vitro
PHYB-ELF3 interaction in vivo could regulate aspects of PHYB-mediated photomorphogenesis
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Fig 4-A. Hypocotyl length of seedlings grown under dark, white, red, and far-red light condition.
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Fig 4-B Flowering time for wild type and mutant plants under LD or SD growth conditions.
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Fig 5. Genetic interactions of the ELF3 overexpression(ELF3-OX) line with the phyB mutation