Biological characteristics · of 230 mice from 16 different populations (2011 & 2012) Allelic...
Transcript of Biological characteristics · of 230 mice from 16 different populations (2011 & 2012) Allelic...
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Biological
characteristics
of a rodent
species in
expansion
Adrien André (Université de liège, McGill University)
Virginie Millien (Mcgill University)
Johan Michaux (Université de Liège)
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Peromyscus leucopus
Rodent from the Cricetidae
family
Widely distributed across
North America
Principal reservoir for Lyme
disease in North America
(Borreliosis associated with
ticks as vector.)
Currently increasing its
Northern range toward
southern Quebec.(Roy-
Dufresne et al 2013)
Recent Lyme disease cases
reported in the region
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Species distributions
Abiotic factors :
Climate
Soil composition
…
Biotic factors :
resource availability
Pathogens & predators
Inter-specific competition
…
Dynamic!!!
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Diversity Disease Hypothesis
fitness pathogen load genetic diversity
Center Edge
Central-Marginal Hypothesis
genetic diversity
Core EdgeLocation in the distribution
(Eckert et al. 2008)
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Diversity Disease Hypothesis
fitness pathogen load genetic diversity
Center Edge
Central-Marginal Hypothesis
pathogen load genetic diversity
Core EdgeLocation in the distribution
(Eckert et al. 2008)
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Diversity Disease Hypothesis
fitness pathogen load genetic diversity
Center Edge
Central-Marginal Hypothesis
fitness pathogen load genetic diversity
Core EdgeLocation in the distribution
(Eckert et al. 2008)
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Enemy Release Hypothesis
pathogen load
Core EdgeLocation in the distribution
(Keane et al. 2002)
Diversity Disease Hypothesis
fitness pathogen load genetic diversity
Center Edge
Central-Marginal Hypothesis
fitness pathogen load genetic diversity
Core EdgeLocation in the distribution
(Eckert et al. 2008)
Low colonizing potential
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Central-Marginal Hypothesis
fitness pathogen load genetic diversity
Enemy Release Hypothesis
fitness pathogen load
Core EdgeLocation in the distribution
(Keane et al, 2002)
Low colonizing potential
Core EdgeLocation in the distribution
(Eckert et al. 2008)
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Enemy Release Hypothesis
fitness pathogen load
Core EdgeLocation in the distribution
(Keane et al. 2002)
High colonizing potential
Central-Marginal Hypothesis
fitness pathogen load genetic diversity
Low colonizing potential
Core EdgeLocation in the distribution
(Eckert et al. 2008)
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Objectives:
Compare different populations according to their
position within the species range
Genetic diversity related to the immune sytem
Stress level
Global health level
Endo- and exo-parasite diversity
Bacterial diversity
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2013 Sampling
15 sampling sites
140 Shermann traps/site
3 or 4 nights/site
140 Peromyscus
leucopus
+ 200 leucopus tissues
from 2011 & 2012
sampling
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MHC II genetic
Major histocompatibility
complex II
Key role in the immune
system for pathogen
recognition.
38 different alleles out
of 230 mice from 16
different populations
(2011 & 2012)
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Allelic richness9 populations with minimum 11 individuals
Rarefaction method (k=10)
6,5 6,6 6,6 6,4 6,7
5,76,4
7,26,6
ALLELIC RICHNESS
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Stress level evaluation1)Fluctuating Asymmetry
Core Edge
Location in the distribution
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Stress level evaluation1)Fluctuating Asymmetry
0.003
0.006
0.009
0.012
0.015
populations
Flu
ctu
ati
ng
as
ym
me
try
Kruskal-wallis test :
P-value>0,05
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Stress level evaluation2) Hair corticosterone
0.00
0.02
0.04
populations
Hair
co
rtic
oste
ron
e
Kruskal-wallis test :
P-value>0,05
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Body condition index(residuals of the regression from the body
mass on the body size)
0.2
0.3
populations
Bo
dy C
on
dit
ion
In
de
x
Kruskal-wallis test :
P-value>0,05
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Central-Marginal Hypothesis
fitness pathogen load genetic diversity
Enemy Release Hypothesis
fitness pathogen load
Core EdgeLocation in the distribution
(Keane et al, 2002)
Core EdgeLocation in the distribution
(Eckert et al. 2008)
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Spleen mass
10
20
30
populations
Co
rre
cte
d s
ple
en
ma
ss
Kruskal test p-value = 0,0018
( P-values from wilcoxon rank sum tests between « parc des chutes »
and other populations < O.O5 )
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TAKE HOME MESSAGE
Central V.S. External populations :
No differences in :
Genetic richness
Stress levels
Body condition index
Smaller spleens in northern
populations
No biological reason yet for the mice to stop their northward expansion!
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TAKE HOME MESSAGE
Central V.S. External populations :
No differences in :
Genetic richness
Stress levels
Body condition index
Smaller spleens in northern
populations
No biological reason yet for the mice to stop their northward expansion.
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Acknowledgement
Funding :This work is supported by a Belgian research fellowship from
the FRIA (Fonds pour la Formation et la Recherche dans
l’Industrie et dans l’Agriculture), financial grants from the
Belgian FNRS (crédits bref séjour et crédits aux chercheurs to
J.R. Michaux), from the University of Liège (Patrimoine),and
from the NSERC fellowship to V. Millien.
Special thanks to
-Alice Mouton
-Maxime Galan
-all the field and lab collaborators
-all my friends and family
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Additional results
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Groups based on occurrence of the 38 alleles in
the 16 populations
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Leucopus presence : (Roy-Dufresne et al 2013)
• Blue dots: 1975-1984
• Red dots :1985-1994
• Yellow dots :1994-2004
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parasite screening
Exoparasites :
Ticks, fleas, botflies
No effect on stress level or body condition index.
Botfly presence directly related to spleen mass
Endoparasites :
25 screened samples out of 125
6 parasited mice
5 with Syphacia
1 with trematode.
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MHC Phylogeny
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Dendogram based on occurrence of the 38 alleles in the
16 populations.
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Groups based on occurrence of the 38 alleles in the 16
populations
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Groups based on occurrence of the 38 alleles in the 16
populations
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Groups based on occurrence of the 38 alleles in the 16
populations
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Dendogram based on occurrence of the 38 alleles in the
16 populations.
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Phylogeny (Maximum likelihood tree ):
Evidence for trans-species polymorphism
(work in progress)
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Spleen mass:
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Spleen mass:
Adjusted R-squared: 0.1694p-value: 1.837e-05
Body condition index:
residues from the
regression of the body
mass on the body size.
= general health index
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Spleen mass:
Individuals with big spleens are whether :
• Bot-flies parasited miceneed to invest energy in the immune system to fight the
parasites or their negative impact.
• Generally healthy miceinvestment of the extra energy in the immune system for
potential futur infections.