Bioengineering the Future with Shell Oil
description
Transcript of Bioengineering the Future with Shell Oil
![Page 1: Bioengineering the Future with Shell Oil](https://reader036.fdocuments.us/reader036/viewer/2022062322/5681488e550346895db5a8a2/html5/thumbnails/1.jpg)
Bioengineering the Futurewith Shell Oil
![Page 2: Bioengineering the Future with Shell Oil](https://reader036.fdocuments.us/reader036/viewer/2022062322/5681488e550346895db5a8a2/html5/thumbnails/2.jpg)
Transport Fuels
75% of liquid fuels are used for transport. 70% of that in cars.
Retail fuel is 95% fossil alkanes and 5% from a biological source.
By 2020, global demand for bio-derived fuels will be 500B litres.
Wood Image
![Page 3: Bioengineering the Future with Shell Oil](https://reader036.fdocuments.us/reader036/viewer/2022062322/5681488e550346895db5a8a2/html5/thumbnails/3.jpg)
The Exeter-Shell Projects
Genes for Alkane Production inHigher Plants
Inducible Wax SynthesisIn Salt Cress
Development of High-throughputMutant Analysis and Wax Imaging
Molecular Control of HCProduction & Secretion in Botryococcus braunii
B. braunii-BacterialInteractions
B. Braunii GenomeEvolution
Botryococcus braunii Culture SystemsProductivity & HC Extraction
Synthetic Ecologyfor Optimum Algal Growth
HC Synthesis Screen in Oleaginous Yeasts
Rhodosporidium toruloidesGenome Analysis
High-throughputScreen Development
Alkane Production & Secretionin Vibrio furnissii
Alkane Synthesis incyanobacteria
Alkane Synthesis inDesulfovibrio
FA Catabolism incyanobacteria
Alkane Synthesis inMycobacterium
Forced Evolution& Mutagenesis for
Increased FA
Bacterial Population Samplingfor HC Production
Molecular & HC AnalysisOf Isolated Mutants
Molecular Control of Hydrocarbon Synthesis & Secretion in Bacteria, Yeast and Algae
for Advanced Biofuel Production
Metabolism &Enzymes
Gene & PathwayDiscovery
MolecularBioinformatics
Synthetic Biology
HC Analysis
Culture Systems& DSP
C-Flux EngineeringIn YeastVariable Chain Length & Branched HC
in Fermentative Microbes
![Page 4: Bioengineering the Future with Shell Oil](https://reader036.fdocuments.us/reader036/viewer/2022062322/5681488e550346895db5a8a2/html5/thumbnails/4.jpg)
Synthetic Biology
Engineer the production of
gasoline, jet-fuel, diesel and
lubricant oil in E. coli that are
identical to fossil fuels.
Biological Alkanes require
no alteration of current
engine technologies.
acoL
bkdAA &
bkdAB
bkdB
ilvE
fabHB
accA to accD
ILV Keto acid
MDHLA
Keto acyl CoA
Malonyl-ACP
Acetyl CoA
Fatty acyl-ACP
Branched fatty acyl-
ACP
fabH
FFA
F-al
Alkanes &
alkenes
NpAD
luxC & luxE
tesA
FATB1
luxD
FATB1
luxD
![Page 5: Bioengineering the Future with Shell Oil](https://reader036.fdocuments.us/reader036/viewer/2022062322/5681488e550346895db5a8a2/html5/thumbnails/5.jpg)
“Third Generation” Biofuels from Algae
Renewable.
Sustainable.
Productive.
CO2 Mitigation.
Experimental.
![Page 6: Bioengineering the Future with Shell Oil](https://reader036.fdocuments.us/reader036/viewer/2022062322/5681488e550346895db5a8a2/html5/thumbnails/6.jpg)
Botryococcus braunii Oils.
Synthesises and secretes very long chain hydrocarbons.
Major fossil component of oil wells.
![Page 7: Bioengineering the Future with Shell Oil](https://reader036.fdocuments.us/reader036/viewer/2022062322/5681488e550346895db5a8a2/html5/thumbnails/7.jpg)
Botryococcus braunii Research.
Genome sequencing and
bioinformatics to elucidate oil
biosynthetic pathways &
interactions with symbiotic
bacteria.
Quantify B. braunii physiology
for Life Cycle Analysis.
Model and test oil production in
industrial culture conditions.