Biochemistry Lecture 1. Blooms Taxonomy Richard C. Overbaugh, Lynn Schultz Old Dominion University.
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Transcript of Biochemistry Lecture 1. Blooms Taxonomy Richard C. Overbaugh, Lynn Schultz Old Dominion University.
Biochemistry
Lecture 1
Bloom’s Taxonomy
Richard C. Overbaugh, Lynn SchultzOld Dominion University
Student Objectives for this course
• Calculate bioenergetic parameters and evaluate carbon molecules reactions
• Reproduce and explain key metabolic processes: glycolysis, TCA cycle, electron transport and oxidative phosphorylation, fatty acid oxidation
• Analyze protein structure and function and evaluate different methods used to assess and test structure and function
• Trace key metabolites through key pathways, design experiments to test carbon flux
• Compare and contrast modes of metabolism regulation and judge the effects of different modes
Biochemistry is the chemistry of
Living Systems• The Chemistry of Carbon and Water• Themes for this course:
– The transformation of energy– Levels of complexity
Cells
Cells
Levels of Complexity
Lipids
The Inner Life of the Cell
• http://multimedia.mcb.harvard.edu/anim_innerlife.html
Why CarbonBond Strength
(kJ/mol)
C – C 347 – 356
C = C 611
C = C 837
C – O 336
C – H 356 – 460
Si – Si 230
Si – O 368
O – O 146
O = O 498
N – N 163
N = N 418
N = N 946
What can we learn from this?
• C – C bond is stronger than C – O– Stable in oxygen rich
environment!
• Two C – C bonds are stronger than one C = C– Chains are stable!
• C – H bond is strong– Hydrocarbons stable at
room temperature!
Important Functional Groups
• Alcohol• Thiol• Amine• Ether• Thioether• Peroxide• Disulfide• Aldehyde• Ketone
• Carboxylic acid• Ester• Anhydride• Amide• Thioester• Phosphate• Phosphoester• Phosphoanhydride
Carbon and Functional Groups
Other biomolecules
NADP+
PEP
Phosphatidylcholine
Bioenergetics
CellReactants Products
Steady State = constant flux
STHG
QRTGG ln'0
Structural differences between reactants and products
Concentration differences between reactants and products
Water
Hydrogen Bonds
Water
Colligative Properties
pH
pH = -log[H+]
214- M101]OH][H[ wK
14]OHlog[]Hlog[ -
14pOHpH
Acids
Buffers
HA][
]A][H[ -
aKHA H+ + A-
HA][
]A[logppH
-
aK
Hendeson Hasselbalch Equation
Summary
• Biochemistry is the chemistry of living things– Which is the chemistry of carbon and
water
• Carbons unique bonding properties• Water: hydrogen bonds and
ionization• Buffers and pH
