Biochain Cancer miRNA Signature Validation Service
1
Differentiation of cellular subtypes (adenocarcinoma and squamous carcinoma) of NSCLC from normal control tissues Expression heat map of 13 miRNAs in 21 paired Non-Small Cell Lung Cancer (NSCLC) and adjacent normal frozen tissues Cancer miRNA Signature Validation Service Step 1: Step 2: Step 3: Step 4: Step 5: Extract miRNA miRNA probe Design One or mulplex miRNA Assay Reacons Perform Real-Time PCR Amplificaon Analyze Data •BioChain miRNA Isolaon Kit •miRNA Reverse Transcripon Kit •Pro QPCR Master Mix •Real-Time PCR System •RT-PCR /Stascal Analysis •miRNA Assays 30 min 60 min 25 min 90 min A: adjacent normal control; B: NSCLC Sample Preparation for Diagnostics & Life Science FR1307-01 www.biochain.com ~ 1-888-762-2568 (Main) ~ [email protected] (Order)
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Biochain Cancer miRNA Signature Validation Service
Transcript of Biochain Cancer miRNA Signature Validation Service
Differentiation of cellular subtypes (adenocarcinoma and squamous carcinoma) of NSCLC from normal control tissues
Expression heat map of 13 miRNAs in 21 paired Non-Small Cell Lung Cancer (NSCLC) and adjacent normal frozen tissues
Cancer miRNA Signature Validation Service
Step 1: Step 2: Step 3: Step 4: Step 5:Extract miRNA miRNA probe
DesignOne or multiplex miRNA Assay Reactions
Perform Real-TimePCR Amplification
Analyze Data
•BioChain miRNA Isolation Kit
•miRNA Reverse Transcription Kit
•Pro QPCR Master Mix •Real-Time PCR System •RT-PCR /Statistical Analysis
•miRNA Assays
30 min 60 min 25 min 90 min
A: adjacent normal control; B: NSCLC
Sample Preparation for Diagnostics & Life Science
FR1307-01
www.biochain.com ~ 1-888-762-2568 (Main) ~ [email protected] (Order)
