Marine Turtles in Bahrain Khawla Al Muhannadi President of Environment Friends Society.
Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz...
Transcript of Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz...
![Page 1: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/1.jpg)
BioBio--FunctionalizedFunctionalizedSurfacesSurfaces
![Page 2: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/2.jpg)
Hard / Soft Interfaces
Electronic structure, absorption and reactivity properties are tunable
• Change due to interface correlations• Ionic multilayers screen fields• Interactions with Applied Electric Fields • Multiple Dielectric Interfaces
Change the behavior of polymers in the vicinity of the hard, wet surface
![Page 3: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/3.jpg)
Interfaces: Nano Heterogeneity Surfaces, Beads, Shells…
• Changes in Species• Large Changes in Electric Fields • Changes in Density
Natural Place for Chemical Work
![Page 4: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/4.jpg)
![Page 5: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/5.jpg)
Salt Water
Prepared Hard Surface
---
- ---
- - --- - --
-- -
--
--
--- - -
-- -
GCTA
ATCGGC
AT
Probes
Targets- ---
-------
T
TT A A
AC
C CG G G
G
5nm
![Page 6: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/6.jpg)
![Page 7: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/7.jpg)
DNA & Protein MicroarraysDNA & Protein Microarraysare useful for a variety of tasks
• Genetic analysis• Disease detection• Synthetic Biology• Computing
![Page 8: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/8.jpg)
Problems in MicroarraysProblems in MicroarraysCross platform comparisons
• Controls• Validation• Data bases for comparisons
Nearly impossible due changes in physics and chemistry at the surface
![Page 9: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/9.jpg)
Central Theoretical Central Theoretical Issue:Issue:
Binding (recognition) is different in the presence of a surface than in
homogeneous solution.
The surface determines: Polarization fields
Ionic screening layers
Ultimately: Device response
![Page 10: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/10.jpg)
Simulations, TheorySimulations, Theoryand Data Processingand Data Processing
• Simulations at the Atomic Level –Detailed, Accurate–Expensive Time consuming
• Theory–Approximate Rules of Thumb
• Processing the Image Data–Must be Fast and Accurate
![Page 11: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/11.jpg)
Forces: Surface and SolutionForces: Surface and Solution
±±±±±
εWater+[salt]
εSubstrate
–– – ––– –
––
–
–
––––
–
– ––
–
–
––
–
–––––
– –
–
––
––
±±±±±±±
±±±
![Page 12: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/12.jpg)
Simulations or Theories of Bio Chips
Set up must include• Substrate (Au, Si, SiO2 …)• Electrostatic fields• Surface modifications• Spacers (organic)• Probe and Target Bio (DNA or protein) strands• Salt and lots of Water
![Page 13: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/13.jpg)
The ChemistryThe Chemistry
Na Cl .1 to .8 M
ProbeTarget
![Page 14: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/14.jpg)
Simulate a simple classical force fieldSimulate a simple classical force field
Model the interactions between atoms• Bonds - 2 body term
– harmonic, Hooke's law spring
• Angles - 3 body term
• Dihedrals - 4 body term
∑ −bonds
eb rrK 2)(
∑ −angles
eK 2)( θθθ
( )∑ ++torsions
nK )cos(1 δφφ Source: http://www.ch.embnet.org/MD_tutorial/
![Page 15: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/15.jpg)
Nonbonded Terms• 2 body terms
• van der Waals (short range) & Coulomb (long range)
• Coulomb interaction consumes > 90% computing timeEwald Sum electrostatics to mimic condensed phase
screening• Periodic Boundary Conditions
rqq
rrji
atomsofpairsNonbonded
ijijij +
−
∑612
4σσ
ε
Electrostatic Forces Dominate Behavior
![Page 16: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/16.jpg)
F = ma or
With a classical Molecular Mechanics potential, V(r)
These potentials have only numerical solutions.
∆t must be small, 10-15s
rmrV &&=∇− )(
)(0)()()()( 32!2
1 tttrttrtrttr ∆+∆+∆+=∆+ &&&
![Page 17: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/17.jpg)
Ewald Fast Multipole Ewald Fast Multipole • Insist on deterministic trajectories• Relative precision ∆Fij<10-6 wrt Ewald• Very fine grain communications
overlapping and inverse message pulling• 40x over optimized Ewald for 100K atoms
![Page 18: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/18.jpg)
Periodic Boundaries for Surfaces: Change symmetry
Skew BCs
![Page 19: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/19.jpg)
![Page 20: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/20.jpg)
ImplicationsImplications• Colloidal behavior affects
– synthesis / fabrication – and binding
• Tilt restricts possible geometries of pairing• Low fraying consistent with high affinity and
good specificity at low target concentration∆G & ∆∆G
![Page 21: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/21.jpg)
A Simple Model• Ion permeable, 20 Å sphere over a plane/surface
– 8 bp in aqueous saline solution over a surface• Linear Poisson-Boltzmann has an
analytic solution
h
Poly - Ohshima and Kondo, ‘93DNA - Vainrub and Pettitt, CPL ’00Ellipse - Garrido and Pettitt, CPC, 07
![Page 22: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/22.jpg)
![Page 23: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/23.jpg)
Longer Sequences are possible
True mesoscale models
![Page 24: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/24.jpg)
The shift of the dissociation free energy or temperature for an immobilized 8 base pair
oligonucleotide duplex at 0.01M NaCl as a function of the distance from a charged dielectric surface
q=0 or ±0.36e-/nm2
SHTm ∆
∆=
linker
![Page 25: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/25.jpg)
Surface at a constant potential for a metal coated substrate @ .01 M NaCl
![Page 26: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/26.jpg)
Response to EResponse to E--fieldsfieldsSalt and Substrate Material Effects on 8-bp DNA
0.1 1 10-100
-50
0
0.1 1 10 0.1 1 10
0.1 1 10
0
50
100
150
200
0.1 1 10 0.1 1 10
DIELECTRIC ϕ = 0
Shi
ft of
mel
ting
tem
pera
ture
∆T
(o C)
DIELECTRIC ϕ = -25 mV
DIELECTRIC ϕ = +25 mV
METAL ϕ = 0
Distance from surface h (nm)
METAL ϕ = -25 mV
0.001M 0.01M 0.1M 1M
METAL ϕ = +25 mV
![Page 27: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/27.jpg)
Finite Concentration and CoverageFinite Concentration and Coverage∇2φ = κ2φ outside the sphere and plane,∇2φ = κ2φ − (ρ/εε0) inside the sphere,
φ|r =a+ = φ|r =a- , r φ|r =a+ = r φ|r =a- on the sphere,φ|z =0+ = φ|r =0- , z φ|z =0+ - r φ|z =0- = -σ/εε0 on the plane.
h
Different from O & K
Vainrub and Pettitt, Biopolymers ’02ibid, NATO Sci , ’05
![Page 28: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/28.jpg)
Coulomb Blockage Dominates Coulomb Blockage Dominates Optimum DNA spacingOptimum DNA spacing
High negative charge density repels target
surface binding
Langmuir
On Chip
Target ConcB
indi
ng
Probe surface density
)+
∆−∆
−=
RTwn
RT
STH
θ
θ TPPP000
ZZZC
θ(exp
1exp
hybridization efficiency θ (0≤ θ ≤1) target concentration C0
Vainrub&Pettitt PRE (2004)
![Page 29: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/29.jpg)
Fit with Experimental IsothermFit with Experimental Isotherm
Accord with experiments:• Low on-array hybridization efficiency (Guo et al 1994, Shchepinov et al 1995)• Broadening and down-temperature shift of melting curve (Forman et al 1998, Lu et al
2002)• Surface probe density effects (Peterson et al 2001, Steel et al 1998, Watterson 2000)
0 5x1012 1x1013 2x1013-15
-14
-13
-12
-11
ln[(1
-θ)C
/θ]
(1+θ)Np
![Page 30: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/30.jpg)
0.0
0.2
0.4
0.6
0.8
1.0
200 250 300 350
Parameter:[(Vd-Vp)/RT0]σ=1
*qp*Np
12 10 8 6 4 2 1 0
Temperature (K)
Hyb
ridiz
atio
n ef
ficie
ncy
Melting curve temperature and widthMelting curve temperature and widthAnalytic wrt surface probe density (coverage)
*1012 nPprobes/cm2
TmTm - ∆Tm
W + ∆W WIn solutionTm = ∆Η0/ (∆S0 – R ln C)
W = 4RTm2/∆Η0
On-array: Isotherms
m
p2
0
p2
m
T32 W
n3wZ H2
n3wZ T
∆=∆
+∆=∆ dA20 /dT20
duplex
Critical for SNP detection design Pettitt et al NATO Sci 206, 381 (2005)
![Page 31: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/31.jpg)
Strength and linearity of hybridization signalStrength and linearity of hybridization signal
109
1010
1011
1012
10-2 10-1 100 101 102 103 104
0.186421
Target concentration (*exp[∆G0/RT0] Moles)
Hyb
rids
dens
ity (1
/cm
2 )
0 5x1012 1x1013
0
2x1010
Probe density (1/cm2)
x1012
Vainrub and Pettitt JACS (2003); ibid Biopolymers, (2004)
![Page 32: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/32.jpg)
Peak of sensitivity also AnalyticPeak of sensitivity also Analytic
0.0 2.0x1012 4.0x10120.0
0.2
0.4
0.6
0.8
1.0
T = 360 K
T = 340 K
T = 334 K
T = 332 K
T = 330 K
Nor
mal
ized
hyb
ridiz
atio
n si
gnal
Probe surface density (cm-2)
2P
p wZRTn =
Vainrub and Pettitt,JACS (2003)
![Page 33: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/33.jpg)
Abs
olut
e de
nsity
dis
tribu
tion
Density Waves at a +Density Waves at a +veve charged Surfacecharged Surface
No simple double layer.Rich multi-layer structure.
Not Poisson-Boltzmann field!
![Page 34: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/34.jpg)
We Have Strong We Have Strong CorrelationsCorrelations
• Concentration is a poor variable• Activity is required• Many non mean field
correlations are important• Multiple length scales
competing
![Page 35: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/35.jpg)
To design for To design for Affinity and SpecificityAffinity and Specificity
• Use Electric fields –Effects of DNA with poly cations
• Use surface effects–Layered hard materials
• Use more quantitative theories –non m.f.
![Page 36: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/36.jpg)
ConclusionConclusionTo control the surfaces we must use cleaner
environments:
Micro and nano features for bio chips deserve the same standards as the computer chip
industry
Clean rooms with wet and dry facilitiesBio + Nano
![Page 37: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/37.jpg)
![Page 38: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/38.jpg)
Arnold VainrubClem WongMichael Feig
Wilfredo OrtizKhawla Qamhieh
Alex MicuKeck Center for Computational Biology
Mike Hogan, Lian Gao, Rosina Georgiadis,
Yuri FofanovThanks to NIH, NASA, DOE, Welch Foundation, ARP&SDSC, PNNL, PSC, NCI, MSI
![Page 39: Bio-Functionalized Surfaces...Bio + Nano. Arnold Vainrub Clem Wong Michael Feig Wilfredo Ortiz Khawla Qamhieh Alex Micu Keck Center for Computational Biology Mike Hogan, Lian Gao,](https://reader033.fdocuments.us/reader033/viewer/2022050206/5f59a178f178c95afc105cf5/html5/thumbnails/39.jpg)