BIO 175 LAB TEST I – STUDY GUIDE

What type of microscope is used in lab?____________________________________How do you properly carry a microscope. _____________________________________What is used to properly clean microscope lenses in OUR lab? _____________________What objective is used most by a microbiologist? _______________________________Identify parts of the microscopeWhich adjustment knob is used to sharpen the image on high – dry examination? Define parfocal Why is immersion oil used on the 100X objective lens. If you are getting 400 X magnification with a 40 X high dry objective, what is the power of the ocular? Laboratory microscope oculars have the magnification ofThe total magnification of an image with the low power objective and the 10 X oculars is Describe step-by-step the preparation of a smear from a liquid media.Name reasons for heat-fixing the slide after the smear is air-dried.What is the disinfectant used in our lab ?_________________________________ In making an aseptic transfer what is the FIRST STEP in the transfer? How do you prepare a slide before transferring the microbe to the slide? Why is it best to hold a slide between your fingers when passing the slide through a Bunsen burner flame to heat-fix the slideIn the handling culture lab what microbe was used._______________________________How did the bacterium appear when growing in broth or on slant? At what temperature must S. marcescens be grown for pigmentation to occur.The Gram stain separates bacteria into how many bacterial groups?Why do Gram positive bacteria retain the crystal violet dye?Which dye stains the gram positive bacteria.____________________________________Which dye stains the gram negative bacterium. _________________________________What is a mordant.Give an example of a mordant.________________________________What is the decolorizing agent used in lab?___________________________________What effect does aging have on a bacterial culture and Gram staining?Name microbes that was gram positive in lab . _________________________________Name microbes in lab that was gram negative. ________________________________Name a genus of bacteria that are endospore formers. __________________________Name something that an endospore is resistant to______________________________What is done first and last to your lab table each time?What is a pure culture?How do most microorganisms exist in the environment?Name microbes used in the Mixed Broth. What is the name of the machine used to stir broth media? Why is it necessary to isolate individual colonies from a mixed culture? Describe an aseptic transfer step–by-step. Describe the streak dilution technique used in lab step-by-step.