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Bials Orcinol TestGroup 5Algarme Dablo Garde Provida Solano Yu

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Background

Pentose is amonosaccharide withve carbon atoms

Hexose is amonosaccharide with

si! carbon atoms"#lose $#!ose

Arabinose %ibose

Galactose Glucose &

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Pur'ose

To test the 'resence o* 'entose in thesolution

To distinguish 'entoses *rom he!oses

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-om'osition

Orcinol %eagent contains( Orcinol . reacts with deh#drated 'entose to

*orm *ur*ural /#drochloric acid deh#drates 'entose to *orm

*ur*ural with orcinol &erric chloride . gives a bluish.violet coloration

with the a0ueous solution o* orcinol

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Procedure

2 m$625 dro's o* Glucose, &ructose and"#lose is6are added in each o* the 7 testtubes with 2 m$625 dro's o* Orcinolsreagent then warmed in water bath 843until a change in color is observed+

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%esults

S'eci c ob9ective(To determine the

presence of pentoses

-olor o* reagentYellow

Samples Observations

Glucose &rom light #to intense #solution

&ructose :ot tested

"#lose &rom light #to intense #solution

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Positive %esult

Bluish solution &ur*ural *urther reacts with orcinol and the iron ion

'resent in the test reagent to 'roduce a bluish'roduct+

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:egative %esult

Green, %ed, Brown solution /e!oses react to *orm Green, %ed, Brown 'roduct

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-om'arison

Seliwano;)s test is a chemical test whichdistinguishes between aldose and ketose sugars