Best practices in laboratories from the dual perspectives of research and sustainability.
-
Upload
nora-flowers -
Category
Documents
-
view
213 -
download
0
Transcript of Best practices in laboratories from the dual perspectives of research and sustainability.
![Page 1: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/1.jpg)
best practices in laboratoriesfrom the dual perspectives of
research and sustainability
![Page 2: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/2.jpg)
best practices: laboratory equipment
fume hoods
ultra-low freezers
lasers
microscopes
large centrifuges
heating blocks
tissue culture hoods
autoclaves
deionized, distilled, and ultrapure water
![Page 3: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/3.jpg)
plastics
gloves
animal bedding
best practices: laboratory consumables
![Page 4: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/4.jpg)
a brief conceptualization of energy consumption
30 kWh/day
![Page 5: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/5.jpg)
fume hoods
wikipedia.com
![Page 6: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/6.jpg)
fume hoods
greenfumehood.com
![Page 7: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/7.jpg)
fume hoods
classification
velocity of airflow through the hood
uses of the hood
ducted v ductless
![Page 8: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/8.jpg)
fume hoods: how it works
ehs.ufl.edu
![Page 9: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/9.jpg)
fume hoods: how it works
web.princeton.edu
![Page 10: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/10.jpg)
fume hoods: constant air volume (CAV)
exhaust flowrate or quantity of air pulled through the hood is constant
oregonstate.edu
![Page 11: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/11.jpg)
fume hoods: variable air volume (VAV)
exhaust flowrate or quantity of air pulled through the hood varies
southwestsolutions.com
![Page 12: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/12.jpg)
fume hoods
energy consumption
3x typical household-Lawrence Berkeley Labs
![Page 13: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/13.jpg)
‘be good in the hood’
![Page 14: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/14.jpg)
![Page 15: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/15.jpg)
‘be good in the hood’
ALWAYS close the sash
thermoscientific.com
safest position.
![Page 16: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/16.jpg)
‘be good in the hood’
do not store unnecessary items in the hood
![Page 17: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/17.jpg)
‘be good in the hood’
being good in the fume hood not only ensures your safetybut it also saves energy
![Page 18: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/18.jpg)
tissue culture hoods
med.cam.ac.uk newvoiceforresearch.blogspot.com
![Page 19: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/19.jpg)
tissue culture hoods: how they work
ars.usda.gov
![Page 20: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/20.jpg)
tissue culture hoods
energy consumption
1 – 12.6 kWh/day depending on usage-manufacturer data
0.3 – 0.6 kWh/day per UV lamp-manufacturer data
![Page 21: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/21.jpg)
‘be good in the hood’
![Page 22: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/22.jpg)
‘be good in the hood’
turn hood COMPLETELY off
thelabworldgroup.com
prevents UV damage toreagents and staff
![Page 23: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/23.jpg)
‘be good in the hood’
being good in the tc hood not only ensures your safetyand prevents unnecessary UV damage to hood contents
but it also saves energy
![Page 24: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/24.jpg)
ultra-low freezers
![Page 25: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/25.jpg)
ultra-low freezers: -80
energy consumption
15 – 20 kWh/day-manufacturer data
-validated by monitoring
![Page 26: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/26.jpg)
‘-70 is the new -80’
![Page 27: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/27.jpg)
‘-70 is the new -80’
chill up freezers from -80 to -70
save 2-4kW per day
![Page 28: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/28.jpg)
DOE
‘-70 is the new -80’
![Page 29: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/29.jpg)
clean freezer coils, regularly defrost freezer
save 12-25% energy
other best practices for ultra-low freezers
prolong lifetime of the freezer
![Page 30: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/30.jpg)
perform scheduled maintenance on freezers
other best practices for ultra-low freezers
prolong lifetime of the freezer
![Page 31: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/31.jpg)
remove old, unneeded samples
other best practices for ultra-low freezers
fewer samples stored, fewer freezers are needed
![Page 32: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/32.jpg)
transfer samples to other storage paradigms
-20 freezers
ambient temperature storage containers
other best practices for ultra-low freezers
fewer samples stored, fewer freezers are needed
![Page 33: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/33.jpg)
lasers
laserfest.org
![Page 34: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/34.jpg)
lasers: types
gas lasers
solid state
![Page 35: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/35.jpg)
lasers: gas
energy consumption
15 – 120 kWh/day-manufacturer data
![Page 36: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/36.jpg)
exchange gas lasers for solid state lasers
could save a minimum of 50% energy-manufacturer data
best practices for lasers
solid state lasers last longer, lower maintenance costs
![Page 37: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/37.jpg)
prolongs lifetime of the laser
saves energy
best practices for lasers
turn off (gas) lasers when not in use, especially over the weekend
![Page 38: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/38.jpg)
microscopes
![Page 39: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/39.jpg)
mercury-free microscopy
![Page 40: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/40.jpg)
mercury/metal halide bulbs
mercury-free microscopy
![Page 41: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/41.jpg)
mercury/metal halide bulbs
5000 hours/mg Hg 2.5 hours/mg Hg
1 bulb2000 bulbs
mercury-free microscopy
![Page 42: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/42.jpg)
mercury-free microscopy
recycle used mercury and metal halide bulbs
![Page 43: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/43.jpg)
energy consumption
illumination: 0.12 – 6 kWh/day-monitored by My Green Lab
other best practices for microscopy
![Page 44: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/44.jpg)
exchange mercury/metal halide for solid state light sources
savings 25 – 90%-monitored by My Green Lab
other best practices for microscopy
less toxic, cost less to own, in many cases are better for samples
![Page 45: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/45.jpg)
cost of ownership of microscope illumination sourcesMERCURY ARC METAL HALIDE SOLID STATE
Replacement bulbs 150 15 n/a
Total Bulb Costs $22,500 $10,500 $0
Total Liquid Light Guide Costs
n/a $5,250 $0
![Page 46: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/46.jpg)
LED comparison with metal halide: stability
![Page 47: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/47.jpg)
LED comparison with metal halide: ‘brightness’
mh_dapi ss_dapi mh_gfp ss_gfp mh_y5 ss_y50
50
100
150
200
250
metal halide (300hrs) v. sold state
![Page 48: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/48.jpg)
LED comparison with metal halide: energy consumption
![Page 49: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/49.jpg)
other best practices for microscopy
turn off microscope equipment when not in use
prolongs lifetime of the equipment
saves energy
![Page 50: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/50.jpg)
large centrifuges
djblabcare.co.uk
![Page 51: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/51.jpg)
large centrifuges
energy consumption
0.7 – 6 kWh/day-manufacturer data
![Page 52: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/52.jpg)
store rotors at 4C
energy savings minimum 10%
best practices for large centrifuges
saves time
![Page 53: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/53.jpg)
turn centrifuge off after use
savings minimum 10%
best practices for large centrifuges
prolongs lifetime
![Page 54: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/54.jpg)
heating blocks
phenixresearch.com
![Page 55: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/55.jpg)
heating blocks
energy consumption
0.5 – 12 kWh/day-S-labs
![Page 56: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/56.jpg)
best practices for heating blocks
turn them off when not in use
energy savings minimum 10%
prolongs lifetime
improves safety
![Page 57: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/57.jpg)
autoclaves
oh.water.usgs.gov
lightlabsusa.com
![Page 58: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/58.jpg)
autoclaves: types
stand-alone
bench-top
![Page 59: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/59.jpg)
autoclaves
water consumption
90 gallons hot water per cycle
22 gallons cold water for effluent cooling-UCSB
![Page 60: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/60.jpg)
autoclaves
University of Edinburgh
autoclaves accounted for41% of the total energy
usage on campus
energy consumption
![Page 61: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/61.jpg)
combine loads
use a smaller, research –grade autoclave
best practices for autoclaves
keep autoclave in ‘standby’ rather than ‘on’
![Page 62: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/62.jpg)
water
tap water
distilled water
deionized water
ultrapure water
![Page 63: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/63.jpg)
water
tap water
distilled water
deionized water
ultrapure water
purified
![Page 64: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/64.jpg)
distilled water (ddH2O)
www.stuart-equipment.com
energy and water intense
![Page 65: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/65.jpg)
deionized water (DI)
pre-treatment
reverse osmosis
polishing (deionization)
UV sterilization, final filtration
usually stored
50% efficient
![Page 66: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/66.jpg)
ultrapure water:Millipore (MilliQ)
goes through a filter
35-50% efficient, larger systems more efficient-UCSB
![Page 67: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/67.jpg)
only use what is necessary – keep in mind that what you use is just a fraction of the total water being used
recycle filter cartridges through Millipore
best practices for water consumption
![Page 68: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/68.jpg)
consumables
![Page 69: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/69.jpg)
plastics
![Page 70: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/70.jpg)
plastics
caissonlabs.com labnetinternational.com
![Page 71: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/71.jpg)
plasticswaste stream
est 12% is recycled
every year est 1100 square miles of plastic leave labs-Mt. Baker Bio
![Page 72: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/72.jpg)
recycle plastics that can be recycled
best practices for plastics
utilize vendor recycling programs
use biodegradable plastics
use only what is needed
![Page 73: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/73.jpg)
VWR: TerraCycle for all pipet tip boxes
vendor recycling programs
Genessee Scientific: pipet tip boxes only from GS
Mt. Baker Bio: pipet tip boxes, reagent bottles
![Page 74: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/74.jpg)
gloves
wikipedia.org
![Page 75: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/75.jpg)
gloveswaste stream
1000s of pounds – not well documented
![Page 76: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/76.jpg)
reuse gloves when possible
best practices for gloves
glove recycling program through Kimberly-Clark
![Page 77: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/77.jpg)
animal bedding
drsforstersmith.competcity.com.au
![Page 78: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/78.jpg)
animal beddingwaste stream
usually sent to a landfill
Emory University produces 22 tons/month
![Page 79: Best practices in laboratories from the dual perspectives of research and sustainability.](https://reader035.fdocuments.us/reader035/viewer/2022062713/56649ced5503460f949ba5d1/html5/thumbnails/79.jpg)
compost
best practices for animal bedding
“bedder” animal bedding