Berry Full of DNA:Exploring Properties of Strawberry DNA

Honors Coordinated Science IIWheatley-Heckman

Background

• Every living thing contains cells, and these cells contain DNA.

• Using the right procedures, we can extract DNA from the cells of almost any living thing.

• Examples: The Human Genome Project, Genetic Testing, Crime Scene Investigations…

• http://learn.genetics.utah.edu/content/labs/extraction/

Why Strawberries?

• While human cells contain 2 sets of DNA…strawberry cells contain 8!! That is A LOT of DNA, and it is easy to see when extracted.

• Strawberries are easy to mash, and ripe strawberries produce enzymes that contribute to the breakdown of cell walls.

Procedure Overview

• First, you will break the cells apart by physically crushing the strawberries in a bag.

Procedure Overview• You will use an

extraction buffer (detergent solution) to dissolve the membranes of the cell.

• Then, you will filter the dissolved cells using a funnel and cheesecloth to remove unwanted particles.

• Ethanol is used to observe the DNA.

Important Notes

• Before beginning the lab, answer all the pre-lab questions.

• Read the procedure carefully before starting the lab.

• Wear safety goggles!• Each table should work as 2 groups.• When finished with the lab, answer

all the analysis questions.

What did we see?