Benchtop Flow Cytometry guava® easyCyte · PDF fileTypical Cells Per Protocol: 105 - 106...

download Benchtop Flow Cytometry guava® easyCyte · PDF fileTypical Cells Per Protocol: 105 - 106 cells/test Traditional Microcapillary aste Sample Fluid Laser Tip Waste Laser ... 4000-0040

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Transcript of Benchtop Flow Cytometry guava® easyCyte · PDF fileTypical Cells Per Protocol: 105 - 106...

  • guava easyCyte Systems

    Cell Health Cell Signaling Stem Cells Immunology Cell Counting & Viability Cell Cycle DNA Damage Mitochondrial Analysis Apoptosis

    MARK Pathway EGFR Pathway Pl3/Akt/mTOR Pathway Jak/ATAT Pathway Chemokine

    Embryotic Stem Cell (Human/Mouse) Neural Stem Cell (Rodent)

    Regulation T-Cell Phenotype Markers

    guava easyCyte System microcapillary .

    Benchtop Flow Cytometry

  • Sheath Fluid: NoneWaste:

  • System easyCyte 5 easyCyte 6 easyCyte 6-2L easyCyte 8

    Catalogue 0500-5005 0500-5006 0500-5007 0500-5008

    Type Single tube Single tube Single tube Single tube

    Laser Blue (488nm) Blue (488nm)Blue (488nm) and

    Red (640nm) Blue (488nm) and

    Red (640nm)

    FSC

    SSC

    Green 525/30nm 525/30nm 525/30nm 525/30nm

    Yellow 583/26nm 583/26nm 583/26nm 583/26nm

    Red1 680/30nm 680/30nm 690/50nm 690/50nm

    NIR1 N/A 785/70nm N/A 785/70nm

    Red2 N/A N/A 661/19nm 661/19nm

    NIR2 N/A N/A N/A 785/70nm

    Microcapillary Fluidics

    Direct, Absolute cell counts

    Mixing

    InCyte Software

    guavaSuite Software modules

    Digital Signal Processing

    Microcapillary flow cellWash vial

    Waste vial

    Robotic sample tray

    Up to six-color detection

    Small footprint

    (sheath fluid) Flow cell

    8 80ml waste

    Single tube

    (blue) (blue, red) 6

    : 45.1cm: 44.5cm: 22.2cm

    Specifications for single tube format

    guava easyCyteSingle Sample

  • guava easyCyteHigh Throughput Sampling

    System easyCyte 5HT easyCyte 6HT easyCyte 6HT-2HL easyCyte 8HT

    Catalogue 0500-4005 0500-4006 0500-4007 0500-4008

    Type 96 well 96 well 96 well 96 well

    Laser Blue (488nm) Blue (488nm)Blue (488nm) and

    Red (640nm) Blue (488nm) and

    Red (640nm)

    FSC

    SSC

    Green 525/30nm 525/30nm 525/30nm 525/30nm

    Yellow 583/26nm 583/26nm 583/26nm 583/26nm

    Red1 680/30nm 680/30nm 690/50nm 690/50nm

    NIR1 N/A 785/70nm N/A 785/70nm

    Red2 N/A N/A 661/19nm 661/19nm

    NIR2 N/A N/A N/A 785/70nm

    Microcapillary Fluidics

    Direct, Absolute cell counts

    Automation-96 well and 10 tubes

    Mixing

    InCyte Software

    guavaSuite Software modules

    Digital Signal Processing

    Microcapillary flow cellWash vial

    Waste vial

    Robotic sample tray

    Up to six-color detection

    Small footprint

    (sheath fluid) Flow cell

    8 80ml waste

    96 well plate 10 sample tube

    (blue) (blue, red) 6

    : 51.5cm: 59.0cm: 25.4cm

    Specifications for 96 well format

  • Heat map view

    CASP8

    Staurosporine

    Cam

    ptothecin

    CASP9

    Ann V MitoP

    Software

    96 well plate

    4

    intensity

    Single tube heat map

    Candidate Compound

    guava flexible assay InCyte specific assay guavaSuite ,

    , . QC easycheck guava

    clean .

    InCyte SoftwareInCyte heat-map 11

    . InCyte siRNA screens, apoptosis/cell cycle compound screening, high-throughput cell-based assays

    .

    Heat map (cut-off threshold )

    Heat map single 96-well format 6

    gating drag & drop() plot

    IC

    plot 11 plot

    template

  • www.merck-millipore.comwww.millipore.com/flowcytometry

    ,

    !

    : 02-2140-5408

    Apoptosis Kits

    4500-0450 Guava Nexin Reagent 100 Tests

    4500-0455 Guava Nexin Reagent 500 Tests

    4000-0061 Guava Express 7-AAD Reagent 200 Tests

    4500-0121 Guava TUNEL Kit 100 Tests

    4500-0121-1 Guava TUNEL Kit Bag 1 of 2, (-20C) 100 Tests

    4500-0121-2 Guava TUNEL Kit Bag 2 of 2, (2-8C) 100 Tests

    4500-0500 Guava MultiCaspase SR Kit 100 tests

    4500-0510 Guava Caspase 3/7 SR Kit 100 tests

    4500-0520 Guava Caspase 9 SR Kit 100 tests

    4500-0530 Guava MultiCaspase FAM Kit 100 tests

    4500-0540 Guava Caspase 3/7 FAM Kit 100 tests

    4500-0550 Guava Caspase 8 FAM Kit 100 tests

    4500-0560 Guava Caspase 9 FAM Kit 100 tests

    4500-0570 Guava MultiCaspase SR and Caspase 3/7 FAM Kit 100 tests

    4500-0580 Guava MultiCaspase SR and Caspase 8 FAM Kit 100 tests

    4500-0590 Guava MultiCaspase SR and Caspase 9 FAM Kit 100 tests

    4500-0600 Guava Caspase 3/7 SR and Caspase 8 FAM Kit 100 tests

    4500-0610 Guava Caspase 3/7 SR and Caspase 9 FAM Kit 100 tests

    4500-0620 Guava Caspase 3/7 SR and MultiCaspase FAM Kit 100 tests

    4500-0630 Guava Caspase 9 SR and Caspase 3/7 FAM Kit 100 tests

    4500-0640 Guava Caspase 9 SR and Caspase 8 FAM Kit 100 tests

    4500-0650 Guava Caspase 9 SR and MultiCaspase FAM Kit 100 tests

    FCCH100105 FlowCellect MitoPotential Red Kit 100 tests

    FCCH100106 FlowCellect MitoDamage Kit 100 tests

    FCCH100107 FlowCellect MitoLive Kit 100 tests

    FCCH100108 FlowCellect Annexin Red Kit 100 tests

    FCCH100109 FlowCellect MitoStress Kit 100 tests

    FCCH100109-1 FlowCellect MitoStress Kit, Pkg 1 100 tests

    FCCH100109-2 FlowCellect MitoStress Kit, Pkg 2 100 tests

    FCCH100110 FlowCellect Cytochrome c Kit 100 tests

    FCCH100110-1 FlowCellect Cytochrome c Kit, Pkg 1 100 tests

    FCCH100110-2 FlowCellect Cytochrome c Kit, Pkg 2 100 tests

    FCCH100110-3 FlowCellect Cytochrome c Kit, Pkg 3 100 tests

    Human Immuno Cell Kit

    FCCH100137 FlowCellect Human B-cell FAS Kit

    FCCH100138 FlowCellect Human T-cell Apoptosis Kit

    FCCH100139 FlowCellect Human T-cell MitoDamage Kit

    FCCH100140 FlowCellect Human CD8 T-cell FAS Kit

    FCCH100141 FlowCellect Human T-cell Activation Kit

    FCCH100154 FlowCellect Human CD4 T-cell FAS Kit

    FCCH100155 FlowCellect Human T Cell Caspase 8 Kit

    FCCH100156 FlowCellect Human T Cell Caspase 9 Kit

    FCCH100157 FlowCellect Human T Cell Caspase 3/7 Kit

    CD Kit

    4500-0150 Guava Express CD3/CD4 Kit 100 Tests

    4500-0190 Guava EasyCD8 Reagent Kit 100 Tests

    4500-0195RU0 Guava EasyCD8 Reagent Kit 500 Tests

    4500-0196RU0 Guava EasyCD8 Reagent Kit 1000 Tests

    4500-0480 Guava Auto CD4/CD4% Reagent Kit 100 Tests

    4500-0480RU0 Guava Auto CD4/CD4% Reagent Kit 100 Tests

    4500-0485 Guava Auto CD4/CD4% Reagent Kit 500 Tests

    4500-0485RU0 Guava Auto CD4/CD4% Reagent Kit 500 Tests

    4500-0490 Guava Auto CD4/CD4% Reagent Kit 1000Tests

    4500-0490RU0 Guava Auto CD4/CD4% Reagent Kit 1000 Tests

    FlowCellect Chemokine Kits

    FCXR100420 FlowCellect Chemokine Receptor CXCR1 Surface Expression ID Kit

    100 tests

    FCXR200421 FlowCellect Chemokine Receptor CXCR2 Surface Expression ID Kit

    100 tests

    FCXR300422 FlowCellect Chemokine Receptor CXCR3 Surface Expression ID Kit

    100 tests

    FCXR400423 FlowCellect Chemokine Receptor CXCR4 Surface Expression ID Kit

    100 tests

    FCXR600424 FlowCellect Chemokine Receptor CXCR6 Surface Expression ID Kit

    100 tests

    Cell Toxicity Kit

    4500-0230 Guava Cell Toxicity Kit (EasyCyte)

    4500-0230-1 Guava Cell Toxicity Kit (EasyCyte) Bag 1 of 2 (-20C)

    4500-0230-2 Guava Cell Toxicity Kit (EasyCyte) Bag 2 of 2 (2-8C)

    Cell Cycle Kit

    4000-0040 Guava ViaCount Reagent 100 Tests

    4000-0041 Guava ViaCount Reagent 600 Tests

    4500-0110Guava ViaCount Flex Reagent for Challenging Sample

    100 Tests

    4500-0220 Guava Cell Cycle Reagent 100 Tests

    FCCH025102 FlowCellect Bivariate Cell Cycle Kit for DNA Replication

    FCCH025103 FlowCellect Bivariate Cell Cycle Kit for G2/M Analysis

    4500-0270 Guava Cell Growth Kit 200 Tests

    4500-0270-1 Guava Cell Growth Kit Bag 1 of 2 (-20C) 200 Tests

    4500-0270-2 Guava Cell Growth Kit Bag 2 of 2 (2-8C) 200 Tests

    Human IgG Kit

    4500-0340 Guava RapidQuant Human IgG Kit

    4500-0340-1 Guava RapidQuant Human IgG Kit Part 1

    4500-0340-2 Guava RapidQuant Human IgG Kit Part 2

    4500-0350 Guava Rapid Quant Mouse IgG Kit

    4500-0350-1 Guava RapidQuant Mouse IgG Kit Part 1

    4500-0350-2 Guava RapidQuant Mouse IgG Kit Part 2

    Analysis Kits

    }D


IMV Technologies EasyCyte

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