Beer Oxidation - Amazon S3 · Fat Tire (fresh) Visual: Amber and clear with a moderate amount of...
Transcript of Beer Oxidation - Amazon S3 · Fat Tire (fresh) Visual: Amber and clear with a moderate amount of...
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Beer Oxidation:Chemistry, Sensory Evaluation, and Prevention
Bob Hall
Andy Mitchell
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Who are we?
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Statement of the problem:
Final round AHA judging. 8 judges on 8 flights
40% of beers had oxidation (n=48)
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Chemical definition of oxidation
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C
Carbon has 4 electrons to share when
forming chemical bonds
C can give them away:
Lose Electrons Oxidation
C can take them:
Gain Electrons Reduction
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Carbon forms covalent bonds, i.e, shared
electrons. But that does not imply equal
sharing!
C C
C H
C O
Equal sharing
Electrons nearer to carbon
Oxygen is highly
electronegative and always
takes shared electrons
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C
CC
C
H
H
H
H
H O
H
H
H
H
H
OO
Most reduced C molecule
Provides heat for (Bob’s) wort boil
+4
-4
+3 +1
oxidation state of C
In keg of Fat Tire and your
blood
Most oxidized form of C.
Flows out the airlocks
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CR O
H
H
H+1
CR
O
H-1
Alcohol
CR
O
H
-2 O H
Aldehyde Carboxylic acid
Alcohol oxidation
steps in beer
R is a variable C structure.
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Flavor impacts of oxidation
Too often, attention is paid to relatively few flavour
notes associated with ageing and, of these, cardboard or
wet paper is the most frequently cited. This is hopelessly
limiting, ... (Bamforth 2011)
Dalgliesh 1977
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Oxidation is more than papery!
There are a diverse range of flavors and aroma produced by oxidation
Different things are appropriate for different beers
In our experience papery shows up in 20% or less of oxidized beers
Consumer trials show that drinkers expect certain oxidized aromas in beer!
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Oxidized Aromas
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Beer Style Influences how Oxidation is expressed
Barleywine – Sherry / raisin / dark fruit
Wheat – Mashed potato
Malty Ales – Honey / Golden Grahams
Light Lagers – Papery
Hoppy Beers – Damascanone (red berry)
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• Malt derived compounds• Methional
• Hop derived compounds• β-Damascenone
• Fermentation metabolites• Acetaldehyde
Raw Materials and Oxidation?
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Methional (Malt)
Description: Mashed potato, autolyzed yeast, pet food,
potato chips
Type of Compound: Aldehyde / Thioether
Threshold: 4 parts per billion
Source: Strecker degradation of amino acids
(oxidation)
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Trans-2-nonenal (Malt)
Description: Papery, wet cardboard, lipstick
Type of compound: Aldehyde
Threshold: 50-250 parts per trillion
Source: Oxidation of lipids / fatty acid
Yuck.
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β-Damascenone (Hops)
Description: Red berries, tobacco, “green things turning brown”,
stewed apple
Type of Compound: Rose Ketone
Threshold: 25 parts per billion
Source: Breakdown of hop materials (proportional at amount of
hops used)
Commonly found in grapes
and bourbon
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Acetaldehyde (Ethanol)
Description: Bruised apple, latex paint, pumpkin guts
Type of compound: Aldehyde
Threshold: 5-15 parts per million
Source: Oxidation of ethanol
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Other aromas
• Almond
• Sherry
• Honey
• Candy malt / hops
• Golden Grahams
• Leathery
• Mercaptan / garbage
• Dullness
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Where’s my beer?• Lack of aroma / dullness – oxidation
• Threshold for aroma compounds?• “Good” compounds reduced to below threshold
• “Bad” compounds with low threshold
• Aroma fades from beer
One can detect oxidation
simply by watching
judges attempt to coax
aroma from an entry
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Crown cap study
Oxygen scavenging caps cause beer to become dull as it ages; absorbing all aromas.
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Other aged effects
• Skunky – Storage in fluorescent lights
• Color – Tan and Pink -> Brown
• Loss of foam
• Formation of haze / particulate / chunks. Facilitates gushing.
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What is going on with these beers?
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Control Opened
Sparged
with
pure O2
NBB Pilsener torture
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NBB Pilsener torture
1. Control: untreated Pilsenermedium grainy and bready malt, med-low herbal/spicy hop aroma,
no flaws
2. Opened, purged, and recappedSherry, almond, low paper, dull, candy malt
3. Opened, partially drained, sparged, filled,
recappedVery similar to #2, but more paper. Ugly.
A headspace of air
destroyed this beer!
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Your turn:
Describe the difference between
aged and fresh Fat Tire
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Fat Tire (fresh)
Visual: Amber and clear with a moderate amount of cream-colored foam and light lacing.
Aroma: Nutty, grainy, caramel, and toasty malt character upfront with slight ethyl hexanoate (anise / green apple / black licorice) and grassy, herbal, earthy hop notes in the background.
Taste: Moderate malty sweetness balanced with medium-light bitterness.
Mouthfeel / Body: Smooth dry finish. Medium body.
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Aged Fat Tire
What aromas do you perceive?
Packaged: 01/2017
Warm stored 30 days then cold
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Common Aged Aroma from NBB Expert Taste Panel• Honey
• Sherry
• Raisin
• Dried fruit
• Vegetal / steamed veggies
• Toffee
• Cola
• Concord grape
• Brown / green tea leaves
• Soy sauce
• Potpourri
• Mushroom / earthy / musty
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Transferring, Packaging, and Preventing Oxidation
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Monitoring DO at NBB
• Primary FV ~0 ppb O2 after fermentation
• Empty MV’s (secondary) are purged with CO2, down to under 1.0% O2
• DO meter, inline, after the Centrifuge. Target < 100 ppb.
• The centrifuge, during discharges creates an elevated DO environment. Also higher DO during low flow rates (beginning / end of the run)
• Dry hop addition system can also cause spikes in DO
• Check DO, with the handheld, in the MV. Target < 100 ppb
• DO probes pre-filter. Target < 100 ppb
• These also spike a little during the initial run in and the DAW to beer phase changes.
• On the exit of each of the postfilters/pasteurizers, another meter on the way to the BBTs. We have the ability to send OOS beer to drain, if there are DO (or other issues) ahead of the BBT.
• We would check the DO’s of the BBT beer, via the handheld after filling.
• All the PKG lines have DO meters inline.
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Monitoring DO at NBB
TL;DR: DO < 100 ppb
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How we package at NBB
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Bottle filling
• Pressurize with CO2, vacuum, repeat twice
• FOB / cap on foam
• TPO limits• Force carbed – Stop the line > 250 ppb
• Yeast added – Stop the line > 600 ppb
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Can filling
• Pressurize with CO2, release, repeat
• Seam on foam (as best as possible)
• Add yeast back for many beers
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Yeast can prevent and even reverse oxidation
aromas
Reduces aldehydes to alcohol
Could lower some good aromas (we don’t add
yeast to Citradelic Tangerine or Voodoo Ranger
IPA)
Yeast is the anti-oxidation weapon
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Saison et al.2010
1 year old beer1 year old beer
with new yeast
and sugar
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Transferring beer at home
• Blanket with CO2
• Transfer gently
• Transfer when young, i.e. when yeast are metabolically active
• Consider not transferring beer to a secondary but directly to kegs from primary
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Bottling at home
• Bottle condition. Yeast will reduce oxidized compounds and consume oxygen themselves
• Growler filler (filling off taps with hose)
• Beer gun or counter-pressure bottle fill
Purge bottles with CO2
Fill from bottom
Cap on foam
Do not leave air in headspace!!
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Can we bottle beer with low total packaged
O2? An experimental test
Fill from taps at NBB
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Beer Gun, with and without CO2 purge
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TPO test with fancy O2 sensor
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TPO of air headspace = 13,000 ppb
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Conditioning and Storage
• Condition at fermentation temperature, then:
• Keep beer cold
• Keep beer dark
• Yeast is OK for most beer (very long aging could give soy sauce or meaty aromas, especially in dark beers)
• Drink beer when fresh and send only fresh beer to competitions. Rebrew for AHA second round for some beers.
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Questions?
Bamforth, C. W. (2011). 125th anniversary review: The non‐biological instability of
beer. Journal of the Institute of Brewing, 117(4), 488-497.
Saison, D., De Schutter, D. P., Vanbeneden, N., Daenen, L., Delvaux, F., & Delvaux,
F. R. (2010). Decrease of aged beer aroma by the reducing activity of brewing yeast.
Journal of Agricultural and Food Chemistry, 58(5), 3107-3115.
Vanderhaegen, B., Delvaux, F., Daenen, L., Verachtert, H., & Delvaux, F. R. (2007).
Aging characteristics of different beer types. Food Chemistry, 103(2), 404-412.
Vanderhaegen, B., Neven, H., Verachtert, H., & Derdelinckx, G. (2006). The
chemistry of beer aging–a critical review. Food Chemistry, 95(3), 357-381.
Some further reading: