BEEP

Baltic ESBL Epidemiology Project

Paul NaaberOct 2012, Visby

Diagnostics and education - impact to diagnostic quality* Improvement of screening and confirmation of ESBLs in

Baltic region* Educational activities

Epidemiology, surveillance - impact to public health* Evaluation of ESBLs epidemiology in E. coli and K.

pneumoniae (prevalence, types)

Research* Publications, Master/PhD degrees* Creation of culture collection of resistant E. coli and K.

pneumoniae strains for future studies* Scientific cooperation

Participants (data and strain collection) = 21 LabsCollaborating institution

• Feb 2011 - 1st BARN WS: presentation of project proposal

• March to Sept 2011: Estonian Pilot study (4 labs)

• Nov 2011: 2nd BARN WS: participants meeting, final protocol

• Jan - May 2012: study period

• Introduction of ESBL screening/confirmation methods

• Collection of strains and data in local labs

• Meetings in St. Petersburg and Vilnius

• June-Sept 2012:

• Collection of strains and data to study center

• ID- MALDI-TOF

• Extraction of DNA

• Master students training in Stockholm

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*Estonian pilot study completed (217 strains)*Poster presentation (World Congress of Microbes-2012

Guangzhou, China)

*All 21 BEEP institutions have sent data and strains*Totally 947 ESBL strains: 395 E. coli, 552 K. pneumoniae

*Data checking is ongoing

*DNA extraction and ID confirmation (MALDI-TOF) almost completed

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Esherichia coli Klebsiella pneumoniae

ESBL Out ESBL In ESBL Out ESBL In Carba In

• Lab work• Molecular charactersation of ESBLs

• Seminars, meetings• Estonian meeting Nov 2012• Baltic meeting May? 2013

• Presentations, publications• Abstract to ECCMID

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Thanks to BARN, SMI and all participants

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